SUN3 Gene
protein-coding GIFtS: 45
GCID: GC07M048026
|
|
Sad1 and UNC84 domain containing 3(Previous name: Sad1 and UNC84 domain containing 1 )
(Previous symbol: SUNC1)
| |
Aliases
for SUN3 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| Sad1 And UNC84 Domain Containing 31 2 | | MGC333291 | | SUNC11 2 3 | | Hypothetical Protein MGC333292 | | Sad1 And UNC84 Domain Containing 11 2 | | SUN Domain-Containing Protein 32 | | Sad1/Unc-84 Domain-Containing Protein 12 3 | | |
Export aliases for SUN3 gene to outside databasesPrevious GC identifer: GC07M047912 |
Summaries
for SUN3 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
-- |
Genomic Views
for SUN3 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000007.13 NC_018918.1 NT_007819.17 NT_079592.2
Regulatory elements:
 Search SABiosciences Regulatory transcription factor binding sites for SUN3
Other transcription factors
Search SABiosciences Chromatin IP Primers for SUN3
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat SUN3 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 7p12.3 Ensembl cytogenetic band: 7p12.3 HGNC cytogenetic band: 7p12.3SUN3 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 7 GeneLoc Exon Structure GeneLoc location for GC07M048026: view genomic region
(about GC identifiers)
Start:
|
48,026,745 bp from pter |
End:
|
48,068,716 bp from pter |
Size:
|
41,972 bases |
Orientation:
|
minus strand |
1 alternative location:
| Chr7-,CRA_TCAG 48,066,697-48,108,669 |
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Proteins
for SUN3 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: SUN3_HUMAN, Q8TAQ9 (See
protein sequence)Recommended Name: SUN domain-containing protein 3 Size: 357 amino acids; 40503 Da
Subcellular location: Membrane; Single-pass membrane protein (Potential)
Sequence caution: Sequence=AAP97300.1; Type=Frameshift; Positions=233, 242; Sequence=EAL23808.1; Type=Erroneous genemodel prediction;
Secondary accessions: A4D2F3 D3DVM3 Q4F965 Q7Z4U8Alternative splicing: 2 isoforms: Q8TAQ9-1 Q8TAQ9-2 Explore the universe of human proteins at neXtProt for SUN3: NX_Q8TAQ9
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q8TAQ9
SUN3 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins (2 alternative transcripts):
NP_001025190.1 NP_689995.3
ENSEMBL proteins: ENSP00000388627 ENSP00000297325 ENSP00000406887 ENSP00000378939 ENSP00000395392 ENSP00000409077 ENSP00000387525 ENSP00000410204
Human Recombinant Protein Products:
Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0016021 | integral to membrane |
IEA | -- | | GO:0034993 | SUN-KASH complex |
IEA | -- |
SUN3 for ontologies About GeneDecksing
SUN3 Antibody Products:
Assay Products for SUN3: |
Protein
Domains /
Families
for SUN3 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
SUN3 for domains About GeneDecksing
1 InterPro domain/family:Graphical View of Domain Structure for InterPro Entry Q8TAQ9ProtoNet protein and cluster: Q8TAQ9 1 Blocks protein family: IPB012919 Sad1/UNC-like
UniProtKB/Swiss-Prot: SUN3_HUMAN, Q8TAQ9Similarity: Contains 1 SUN domain |
Function
for SUN3 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for SUN3 (see all 3)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for SUN3 (see all 2)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector: SUN3 (NM_022072) | |  | Browse Sino Biological Human cDNA Clones | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for SUN3 | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat SUN3 |
In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for SUN3 |
Gene Ontology (GO): 1 molecular function term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0005515 | protein binding |
-- | -- |
SUN3 for ontologies About GeneDecksing
|
Pathways & Interactions
for SUN3 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for SUN3
STRING Interaction
Network Preview (showing 5 interactants - click image to see more details)
5 Interacting proteins for SUN3 (ENSP000002973254) via UniProtKB, MINT, STRING, and/or I2DAbout this table
|
Drugs & Compounds
for SUN3 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for SUN3
Search CenterWatch for drugs/clinical trials and news about SUN3
|
Transcripts
for SUN3 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for SUN3 gene (2 alternative transcripts): NM_001030019.1 NM_152782.3 Unigene Cluster for SUN3: Sad1 and UNC84 domain containing 3 Hs.406741 [show with all ESTs]Unigene Representative Sequence: AK30201110 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000461953 ENST00000473723 ENST00000453071 ENST00000297325(uc003tog.3)
ENST00000412371 ENST00000395572 ENST00000449896 ENST00000438771 ENST00000453192(uc011kcf.2)
ENST00000412142(uc010kyq.3 uc003tof.3)
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for SUN3 (see all 3)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for SUN3 (see all 2)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector: SUN3 (NM_022072) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for SUN3 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat SUN3 |
Additional cDNA sequence: AF429967.1 AK302011.1 BC026189.2 DQ099386.1 4 DOTS entries: DT.92417580 DT.309219 DT.100743422 DT.99970208 24/28 AceView cDNA sequences (see all 28): BU623138 BG184281 BI825617 BI829140 AA813611 BP369275 BX119032 BM554058 BI827929 NM_152782 BI829985 AI917508 AI688144 BG772576 AI017490 AA883877 AF429967 BC026189 AA973064 BP369413 AI208514 BG720666 BG703482 AW182987
GeneLoc Exon Structure
3 Alternative Splicing Database (ASD) splice patterns (SP) for SUN3 About this scheme
| ExUns: | 1a | · | 1b | ^ | 2 | ^ | 3 | ^ | 4 | ^ | 5 | ^ | 6 | ^ | 7 | ^ | 8 | ^ | 9 | ^ | 10 |
|---|
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| SP1: | | | | | | | - | | | | | | | | | | | | | | | | |
| SP2: | | | | | | | | | | | | | | | | | | | | | | | |
| SP3: | | | | | | | | | | | | | | | | | | | | | | |
ECgene alternative splicing isoforms for SUN3
|
Expression
for SUN3 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| SUN3 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: TCCAGAATAT
About this image
See SUN3 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for SUN3
SOURCE GeneReport for Unigene cluster: Hs.406741
SABiosciences Custom PCR Arrays for SUN3
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for SUN3
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat SUN3 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat SUN3 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat SUN3 | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for SUN3 |
Orthologs
for SUN3 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of eukaryotes.
Orthologs for SUN3 gene from 5/15 species (see all 15) About this table
| Organism |
Taxonomic
classification |
Gene |
Description |
Human
Similarity |
Orthology
Type |
Details |
chicken
(Gallus gallus) |
Aves |
LOC7704051 |
SUN domain-containing protein 3-like |
57.85(n)
43.92(a) |
|
770405 XM_001233743.2 XP_001233744.2 |
rainbow trout
(Oncorhynchus mykiss) |
Actinopterygii |
BX077792.12 |
-- |
72.44(n) |
|
BX077792.1 |
fruit fly
(Drosophila melanogaster) |
Insecta |
spag46 |
sperm-associated antigen 4 |
14(a) |
possible ortholog |
2L(11610510-11611772) |
thale cress
(Arabidopsis thaliana) |
eudicotyledons |
SUN26ATSUN16 |
SAD1/UNC-84 domain protein 1 |
16(a)15(a) |
many ↔ manymany ↔ many |
3(3358328-3360682) 5(1471481-1473964) |
rice
(Oryza sativa) |
Liliopsida |
---- |
Sad1 / UNC-like C-terminal domain containing prote...Sad1 / UNC-like C-terminal domain containing prote... |
17(a)16(a) |
many ↔ manymany ↔ many |
1(9178212-9180984) 5(10883292-10886698) |
ENSEMBL Gene Tree for SUN3 (if available)
TreeFam Gene Tree for SUN3 (if available) |
Paralogs
for SUN3 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for SUN3 gene
- SPAG42 SUN22 SUN12 SUN52
18/58 SIMAP similar genes for SUN3 using alignment to 5 protein entries: SUN3_HUMAN (see all proteins)
(see all similar genes):LGALS3 FAM3A BUB1B GNPTAB NSRP1 THADA
ZNF23 ARV1 ATL2 C11orf80 C12orf23 COX7A2
DAPK2 LAMA5 LRTOMT MKRN1 TMEM67 UTY
SUN3 for paralogs About GeneDecksing
|
Genomic Variants
for SUN3 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 7 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for SUN3 (48026745 - 48068716 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for SUN3: -- | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing SUN3 |
|
Disorders
/ Diseases
for SUN3 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
SUN3 for disorders About GeneDecksing
2  diseases for SUN3: About MalaCardsamyotrophic lateral sclerosis lateral sclerosis
Human Genome Epidemiology (HuGE) Navigator: SUN3 (0 documents)
Export disorders for SUN3 gene to outside databases
|
Publications
for SUN3 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for SUN3 gene integrated from 9 sources:
(articles sorted by number of sources associating them with SUN3) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- Human chromosome 7: DNA sequence and biology. (PubMed id 12690205)1, 2 Scherer S.W.... Tsui L.-C. (2003)
- Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PubMed id 20379614)1 Rose J.E....Uhl G.R. (2010)
- A two-stage genome-wide association study of sporadic amyotrophic lateral sclerosis. (PubMed id 19193627)1 ChiA^ A....Traynor B.J. (2009)
- Toward a confocal subcellular atlas of the human proteome. (PubMed id 18029348)1 Barbe L....Andersson-Svahn H. (2008)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)2 Gerhard D.S....Malek J. (2004)
- Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)
- Cynomolgus monkey testicular cDNAs for discovery of novel human genes in the human genome sequence. (PubMed id 12498619)1 Osada N....Hashimoto K. (2002)
- Hepatitis C virus core protein interacts with 14-3-3 protein and activates the kinase Raf-1. (PubMed id )2 Aoki H.... Hino O. (2000)
|
External Searches for SUN3 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing SUN3 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing SUN3 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing SUN3 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for SUN3 | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for SUN3 gene:
Search GeneIP for patents involving SUN3
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for SUN3 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
| | |  | Browse OriGene Antibodies | | OriGene shRNA RFP for SUN3 | | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for SUN3 | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for SUN3 | | | OriGene Protein Over-expression Lysate for SUN3 | | Browse OriGene Fluorogenic Cell Assay Kits | | | OriGene siRNA for SUN3 | | Browse 3'-UTR reporter clones for miRNA target validation | | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for SUN3 | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for SUN3 | | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | | OriGene Purified Protein for SUN3 | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling | | | OriGene Custom Antibody Services for SUN3 | | OriGene Custom Protein Services for SUN3 | | | OriGene Custom Immunoassay Development | | |
| |
 |
 |
| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat SUN3 | | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing SUN3 | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat SUN3 | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat SUN3 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat SUN3 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat SUN3 |
| |
 |  |  |  | |
| | | | Search Tocris compounds for SUN3 |
| |  |  |  |  | | | | |
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 | | | SUN3 Proteins, Antibodies, CLIAs, and ELISAs |
| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for SUN3 |
|  |  |  | | | | ThermoFisher Antibodies for SUN3 |
| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat SUN3 |
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