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Aliases for STK3 Gene

Aliases for STK3 Gene

  • Serine/Threonine Kinase 3 2 3
  • Serine/Threonine Kinase 3 (Ste20, Yeast Homolog) 2 3
  • Mammalian STE20-Like Protein Kinase 2 3 4
  • Serine/Threonine-Protein Kinase Krs-1 3 4
  • STE20-Like Kinase MST2 3 4
  • EC 2.7.11.1 4 63
  • MST-2 3 4
  • KRS1 3 4
  • MST2 3 4
  • Serine/Threonine Kinase 3 (STE20 Homolog, Yeast) 3
  • Serine/Threonine-Protein Kinase 3 3
  • KB-1458E12.1 3
  • EC 2.7.11 63

External Ids for STK3 Gene

Previous GeneCards Identifiers for STK3 Gene

  • GC08M098475
  • GC08M099540
  • GC08M099135
  • GC08M099423
  • GC08M099536
  • GC08M099466
  • GC08M094670
  • GC08M099413

Summaries for STK3 Gene

Entrez Gene Summary for STK3 Gene

  • This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. The N-terminal portion is transported to the nucleus where it homodimerizes to form the active kinase which promotes the condensation of chromatin during apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

GeneCards Summary for STK3 Gene

STK3 (Serine/Threonine Kinase 3) is a Protein Coding gene. Among its related pathways are MAPK signaling pathway and Signaling by GPCR. GO annotations related to this gene include protein serine/threonine kinase activity and protein kinase activity. An important paralog of this gene is STK4.

UniProtKB/Swiss-Prot for STK3 Gene

  • Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1 (By similarity). Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosome, and its ability to phosphorylate CROCC and CEP250. In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on Ser-259. Phosphorylates MOBKL1A and RASSF2. Phosphorylates MOBKL1B on Thr-74. Acts cooperatively with MOBKL1B to activate STK38.

Gene Wiki entry for STK3 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for STK3 Gene

Genomics for STK3 Gene

Regulatory Elements for STK3 Gene

Genomic Location for STK3 Gene

Start:
98,401,403 bp from pter
End:
98,942,827 bp from pter
Size:
541,425 bases
Orientation:
Minus strand

Genomic View for STK3 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for STK3 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for STK3 Gene

Proteins for STK3 Gene

  • Protein details for STK3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q13188-STK3_HUMAN
    Recommended name:
    Serine/threonine-protein kinase 3
    Protein Accession:
    Q13188
    Secondary Accessions:
    • A8K722
    • B3KYA7
    • Q15445
    • Q15801
    • Q96FM6

    Protein attributes for STK3 Gene

    Size:
    491 amino acids
    Molecular mass:
    56301 Da
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    • Homodimer; mediated via the coiled-coil region. Interacts with NORE1, which inhibits autoactivation (By similarity). Interacts with and stabilizes SAV1. Interacts with RAF1, which prevents dimerization and phosphorylation. Interacts with RASSF1. Interacts (via SARAH domain) with isoform 1 of NEK2. Interacts with ESR1 only in the presence of SAV1. Interacts with PKB/AKT1. Forms a tripartite complex with MOBKL1B and STK38. Interacts with RASSF2 (via SARAH domain).

    Three dimensional structures from OCA and Proteopedia for STK3 Gene

    Alternative splice isoforms for STK3 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for STK3 Gene

Proteomics data for STK3 Gene at MOPED

Post-translational modifications for STK3 Gene

  • Phosphorylation at Thr-117 and Thr-384 by PKB/AKT1, leads to inhibition of its: cleavage, kinase activity, autophosphorylation at Thr-180, binding to RASSF1 and nuclear translocation, and increase in its binding to RAF1.
  • Proteolytically cleaved by caspase-3 during apoptosis. Proteolytic cleavage results in kinase activation and nuclear translocation of the truncated form (MST1/N).
  • Ubiquitination at Lys177
  • Modification sites at PhosphoSitePlus

Other Protein References for STK3 Gene

Domains for STK3 Gene

Gene Families for STK3 Gene

Graphical View of Domain Structure for InterPro Entry

Q13188

UniProtKB/Swiss-Prot:

STK3_HUMAN :
  • Q13188
Domain:
  • Contains 1 protein kinase domain.
  • Contains 1 SARAH domain.
Family:
  • Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
genes like me logo Genes that share domains with STK3: view

Function for STK3 Gene

Molecular function for STK3 Gene

GENATLAS Biochemistry: serine threonine kinase 3
UniProtKB/Swiss-Prot CatalyticActivity: ATP + a protein = ADP + a phosphoprotein
UniProtKB/Swiss-Prot EnzymeRegulation: Inhibited by the C-terminal non-catalytic region. Activated by caspase-cleavage. Full activation also requires homodimerization and autophosphorylation of Thr-180, which are inhibited by the proto-oncogene product RAF1. Activated by RASSF1 which acts by preventing its dephosphorylation.
UniProtKB/Swiss-Prot Function: Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1 (By similarity). Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosome, and its ability to phosphorylate CROCC and CEP250. In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on Ser-259. Phosphorylates MOBKL1A and RASSF2. Phosphorylates MOBKL1B on Thr-74. Acts cooperatively with MOBKL1B to activate STK38.
UniProtKB/Swiss-Prot Induction: Activity increases during mitosis.

Enzyme Numbers (IUBMB) for STK3 Gene

Gene Ontology (GO) - Molecular Function for STK3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000287 magnesium ion binding IDA 8566796
GO:0004672 protein kinase activity TAS 10840030
GO:0004674 protein serine/threonine kinase activity IDA 18328708
GO:0004702 receptor signaling protein serine/threonine kinase activity IBA --
GO:0004713 protein tyrosine kinase activity --
genes like me logo Genes that share ontologies with STK3: view
genes like me logo Genes that share phenotypes with STK3: view

Animal Models for STK3 Gene

MGI Knock Outs for STK3:

No data available for Transcription Factor Targeting and HOMER Transcription for STK3 Gene

Localization for STK3 Gene

Subcellular locations from UniProtKB/Swiss-Prot for STK3 Gene

Cytoplasm. Nucleus. Note=The caspase-cleaved form cycles between nucleus and cytoplasm (By similarity). Phosphorylation at Thr-117 leads to inhibition of nuclear translocation. {ECO:0000250, ECO:0000269 PubMed:19525978}.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for STK3 Gene COMPARTMENTS Subcellular localization image for STK3 gene
Compartment Confidence
cytosol 4
nucleus 2
plasma membrane 2
cytoskeleton 1

Gene Ontology (GO) - Cellular Components for STK3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IEA --
GO:0005737 cytoplasm IDA 19525978
GO:0005829 cytosol TAS --
genes like me logo Genes that share ontologies with STK3: view

Pathways for STK3 Gene

genes like me logo Genes that share pathways with STK3: view

Pathways by source for STK3 Gene

1 Qiagen pathway for STK3 Gene
2 Cell Signaling Technology pathways for STK3 Gene
1 BioSystems pathway for STK3 Gene
2 Reactome pathways for STK3 Gene
2 KEGG pathways for STK3 Gene

Gene Ontology (GO) - Biological Process for STK3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001841 neural tube formation IEA --
GO:0003157 endocardium development IEA --
GO:0006468 protein phosphorylation IDA 20086174
GO:0006915 apoptotic process TAS 11278283
GO:0007165 signal transduction TAS 8816758
genes like me logo Genes that share ontologies with STK3: view

Compounds for STK3 Gene

(3) HMDB Compounds for STK3 Gene

Compound Synonyms Cas Number PubMed IDs
Adenosine triphosphate
  • 5'-(tetrahydrogen triphosphate) Adenosine
56-65-5
ADP
  • adenosindiphosphorsaeure
58-64-0
Magnesium
  • Magnesium
7439-95-4

(2) Novoseek inferred chemical compound relationships for STK3 Gene

Compound -log(P) Hits PubMed IDs
threonine 46.5 2
serine 33.1 2
genes like me logo Genes that share compounds with STK3: view

Transcripts for STK3 Gene

Unigene Clusters for STK3 Gene

Serine/threonine kinase 3:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for STK3 Gene

ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6a · 6b ^ 7a · 7b ^ 8a · 8b ^ 9 ^ 10 ^ 11
SP1: - - -
SP2: -
SP3:

Relevant External Links for STK3 Gene

GeneLoc Exon Structure for
STK3
ECgene alternative splicing isoforms for
STK3

Expression for STK3 Gene

mRNA expression in normal human tissues for STK3 Gene

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for STK3 Gene

SOURCE GeneReport for Unigene cluster for STK3 Gene Hs.492333

mRNA Expression by UniProt/SwissProt for STK3 Gene

Q13188-STK3_HUMAN
Tissue specificity: Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues.
genes like me logo Genes that share expressions with STK3: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery and mRNA differential expression in normal tissues for STK3 Gene

Orthologs for STK3 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for STK3 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia STK3 35
  • 99.68 (n)
  • 99.61 (a)
STK3 36
  • 98 (a)
OneToOne
cow
(Bos Taurus)
Mammalia STK3 35
  • 93.79 (n)
  • 99.59 (a)
STK3 36
  • 98 (a)
OneToOne
dog
(Canis familiaris)
Mammalia STK3 35
  • 94.82 (n)
  • 99.38 (a)
STK3 36
  • 98 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Stk3 35
  • 90.06 (n)
  • 97.1 (a)
Stk3 16
Stk3 36
  • 95 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia STK3 36
  • 89 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia STK3 36
  • 96 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Stk3 35
  • 89.37 (n)
  • 96.69 (a)
chicken
(Gallus gallus)
Aves STK3 36
  • 96 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia STK3 36
  • 95 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia stk3 35
  • 81.44 (n)
  • 94.41 (a)
zebrafish
(Danio rerio)
Actinopterygii stk3 35
  • 77.87 (n)
  • 89.42 (a)
stk3 36
  • 88 (a)
OneToOne
wufc19e11 35
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP006044 35
  • 59.43 (n)
  • 68.17 (a)
fruit fly
(Drosophila melanogaster)
Insecta hpo 35
  • 63.43 (n)
  • 73.43 (a)
hpo 36
  • 44 (a)
OneToMany
CG11228 37
  • 51 (a)
worm
(Caenorhabditis elegans)
Secernentea cst-1 36
  • 53 (a)
ManyToMany
cst-2 35
  • 66.9 (n)
  • 73.94 (a)
cst-2 36
  • 55 (a)
ManyToMany
C24A8.4a 37
  • 59 (a)
F14H12.4a 37
  • 54 (a)
C24A8.4b 37
  • 59 (a)
F14H12.4b 37
  • 54 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes SPS1 36
  • 28 (a)
OneToMany
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.1634 35
sea squirt
(Ciona savignyi)
Ascidiacea CSA.8767 36
  • 69 (a)
OneToMany
Species with no ortholog for STK3:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for STK3 Gene

ENSEMBL:
Gene Tree for STK3 (if available)
TreeFam:
Gene Tree for STK3 (if available)

Paralogs for STK3 Gene

Paralogs for STK3 Gene

Pseudogenes.org Pseudogenes for STK3 Gene

genes like me logo Genes that share paralogs with STK3: view

Variants for STK3 Gene

Sequence variations from dbSNP and Humsavar for STK3 Gene

SNP ID Clin Chr 08 pos Sequence Context AA Info Type MAF
rs734034 -- 98,929,466(+) agcct(A/G)gggaa intron-variant
rs745721 -- 98,929,612(+) ACTAC(G/T)GTACT intron-variant
rs1057795 -- 98,579,703(-) CATGA(C/G)GAACA missense, reference
rs1080818 -- 98,854,080(-) tttct(C/T)gcctA intron-variant
rs1374601 -- 98,941,241(-) AAAAG(A/G)CTAAG intron-variant

Structural Variations from Database of Genomic Variants (DGV) for STK3 Gene

Variant ID Type Subtype PubMed ID
nsv831406 CNV Loss 17160897
nsv891218 CNV Loss 21882294
nsv891219 CNV Loss 21882294
esv2737350 CNV Deletion 23290073
nsv891220 CNV Gain 21882294
esv2652228 CNV Deletion 19546169
nsv507477 CNV Insertion 20534489

Relevant External Links for STK3 Gene

HapMap Linkage Disequilibrium report
STK3

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for STK3 Gene

Disorders for STK3 Gene

(2) Novoseek inferred disease relationships for STK3 Gene

Disease -log(P) Hits PubMed IDs
warts 64.2 2
tumors 33.4 6

Relevant External Links for STK3

Genetic Association Database (GAD)
STK3
genes like me logo Genes that share disorders with STK3: view

No data available for UniProtKB/Swiss-Prot for STK3 Gene

Publications for STK3 Gene

  1. Cloning and characterization of a member of the MST subfamily of Ste20-like kinases. (PMID: 8566796) Creasy C.L. … Chernoff J. (Gene 1995) 3 4 23
  2. MST, a physiological caspase substrate, highly sensitizes apoptosis both upstream and downstream of caspase activation. (PMID: 11278283) Lee K.-K. … Yonehara S. (J. Biol. Chem. 2001) 3 4 23
  3. Role of the kinase MST2 in suppression of apoptosis by the proto- oncogene product Raf-1. (PMID: 15618521) O'Neill E. … Kolch W. (Science 2004) 3 4 23
  4. The Ste20-like kinase Mst2 activates the human large tumor suppressor kinase Lats1. (PMID: 15688006) Chan E.H.Y. … Sillje H.H.W. (Oncogene 2005) 3 4 23
  5. MOBKL1A/MOBKL1B phosphorylation by MST1 and MST2 inhibits cell proliferation. (PMID: 18328708) Praskova M. … Avruch J. (Curr. Biol. 2008) 3 4 23

Products for STK3 Gene

  • Addgene plasmids for STK3

Sources for STK3 Gene

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