Set Analyses:
Advanced Search

Advanced Search

 
Search By
Section (entire)
for


 



STK3 Gene

protein-coding   GIFtS: 69
GCID: GC08M099413

Serine/Threonine Kinase 3

(Previous names: serine/threonine kinase 3 (Ste20, yeast homolog))
Alzheimer's & Parkinson's Diseases Congress
  See related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

TryGeneCards Plus

Aliases
Serine/Threonine Kinase 31 2     MST22 3
Serine/Threonine Kinase 3 (Ste20, Yeast Homolog)1 2     STE20-Like Kinase MST22 3
Mammalian STE20-Like Protein Kinase 22 3     EC 2.7.11.13 8
Serine/Threonine-Protein Kinase Krs-12 3     Serine/Threonine Kinase 3 (STE20 Homolog, Yeast)2
KRS12 3     Serine/Threonine-Protein Kinase 32
MST-22 3     EC 2.7.118

External Ids:    HGNC: 114061   Entrez Gene: 67882   Ensembl: ENSG000001043757   OMIM: 6050305   UniProtKB: Q131883   

Export aliases for STK3 gene to outside databases

Previous GC identifers: GC08M098475 GC08M099540 GC08M099135 GC08M099423 GC08M099536 GC08M099466 GC08M094670


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

TryGeneCards Plus

Entrez Gene summary for STK3 Gene:
This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded
protein functions as a growth suppressor. Cleavage of the protein product by caspase removes the inhibitory
C-terminal portion. The N-terminal portion is transported to the nucleus where it homodimerizes to form the
active kinase which promotes the condensation of chromatin during apoptosis. Multiple transcript variants
encoding different isoforms have been found for this gene. (provided by RefSeq, Jan 2012)

GeneCards Summary for STK3 Gene:
STK3 (serine/threonine kinase 3) is a protein-coding gene. Diseases associated with STK3 include soft tissue sarcoma, and conjunctivitis. GO annotations related to this gene include protein serine/threonine kinase activity and protein kinase activity. An important paralog of this gene is STK24.

UniProtKB/Swiss-Prot: STK3_HUMAN, Q13188
Function: Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces
chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling
pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and
promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in
complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory
protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of
YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell
proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of
mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit
tumor formation. Phosphorylates NKX2-1 (By similarity). Phosphorylates NEK2 and plays a role in centrosome
disjunction by regulating the localization of NEK2 to centrosome, and its ability to phosphorylate CROCC and
CEP250. In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its
phosphorylation. Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1
on 'Ser-259'. Phosphorylates MOBKL1A and RASSF2. Phosphorylates MOBKL1B on 'Thr-74'. Acts cooperatively with
MOBKL1B to activate STK38

Gene Wiki entry for STK3 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
About This Section

TryGeneCards Plus
RefSeq DNA sequence:
NC_000008.11  NT_008046.17  NC_018919.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the STK3 gene promoter:
         AhR   STAT5B   C/EBPbeta   FOXF2   MyoD   CUTL1   FOXL1   FOXJ2 (long isoform)   FOXJ2   Hlf   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 3): STK3 promoter sequence
   Search Chromatin IP Primers for STK3

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat STK3


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 8q22.2   Ensembl cytogenetic band:  8q22.2   HGNC cytogenetic band: 8q22.2

STK3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
STK3 gene location

GeneLoc information about chromosome 8         GeneLoc Exon Structure

GeneLoc location for GC08M099413:  view genomic region     (about GC identifiers)

Start:
99,413,631 bp from pter      End:
99,955,055 bp from pter
Size:
541,425 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
About This Section

TryGeneCards Plus

UniProtKB/Swiss-Prot: STK3_HUMAN, Q13188 (See protein sequence)
Recommended Name: Serine/threonine-protein kinase 3  
Size: 491 amino acids; 56301 Da
Cofactor: Magnesium
Subunit: Homodimer; mediated via the coiled-coil region. Interacts with NORE1, which inhibits autoactivation (By
similarity). Interacts with and stabilizes SAV1. Interacts with RAF1, which prevents dimerization and
phosphorylation. Interacts with RASSF1. Interacts (via SARAH domain) with isoform 1 of NEK2. Interacts with ESR1
only in the presence of SAV1. Interacts with PKB/AKT1. Forms a tripartite complex with MOBKL1B and STK38.
Interacts with RASSF2 (via SARAH domain)
4 PDB 3D structures from and Proteopedia for STK3:
4HKD (3D)        4L0N (3D)        4LG4 (3D)        4LGD (3D)    
Secondary accessions: A8K722 Q15445 Q15801 Q96FM6

Explore the universe of human proteins at neXtProt for STK3: NX_Q13188

Explore proteomics data for STK3 at MOPED

Post-translational modifications: 

  • Phosphorylation at Thr-117 and Thr-384 by PKB/AKT1, leads to inhibition of its: cleavage, kinase activity,
    autophosphorylation at Thr-180, binding to RASSF1 and nuclear translocation, and increase in its binding to RAF11
  • Proteolytically cleaved by caspase-3 during apoptosis. Proteolytic cleavage results in kinase activation and
    nuclear translocation of the truncated form (MST1/N)1
  • Ubiquitination2 at Lys177
  • Modification sites at PhosphoSitePlus
  • Selected DME Specific Peptides for STK3 (Q13188) (see all 24)
     IEELRQR  HKESGQV  KAGNILL  DSHTMVK 


    See STK3 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (3 alternative transcripts): 
    NP_001243241.1  NP_001243242.1  NP_006272.2  

    ENSEMBL proteins: 
     ENSP00000390500   ENSP00000429744   ENSP00000428014   ENSP00000429369   ENSP00000428167  
     ENSP00000428295  
    Reactome Protein details: Q13188

    STK3 Human Recombinant Protein Products:

    EMD Millipore Purified and/or Recombinant STK3 Protein
    Browse R&D Systems for human recombinant proteins
    Enzo Life Sciences proteins for STK3
    OriGene Purified Protein for STK3
    OriGene Protein Over-expression Lysate for STK3
    OriGene MassSpec for STK3
    OriGene Custom Protein Services for STK3
    GenScript Custom Purified and Recombinant Proteins Services for STK3
    Novus Biologicals STK3 Proteins
    Novus Biologicals STK3 Lysates
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for STK3

    STK3 Antibody Products:

    EMD Millipore Mono- and Polyclonal Antibodies for the study of STK3
    R&D Systems Antibodies for STK3 (MST2/STK3)
    Cell Signaling Technology (CST) Antibodies for STK3  (MST2)
    OriGene Antibodies for STK3
    OriGene Custom Antibody Services for STK3
    Novus Biologicals STK3 Antibodies
    Abcam antibodies for STK3 (Q13043, Q13188)
    Cloud-Clone Corp. Antibodies for STK3
    ThermoFisher Antibody for STK3
    LSBio Antibodies in human, mouse, rat for STK3

    STK3 Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for STK3
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for STK3
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for STK3
    Cloud-Clone Corp. CLIAs for STK3


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    TryGeneCards Plus
    IUPHAR Guide to PHARMACOLOGY protein family classification: serine/threonine kinase 3
    MST subfamily

    Selected InterPro protein domains (see all 6):
     IPR017441 Protein_kinase_ATP_BS
     IPR011524 SARAH_dom
     IPR002290 Ser/Thr_dual-sp_kinase_dom
     IPR011009 Kinase-like_dom
     IPR000719 Prot_kinase_dom

    Graphical View of Domain Structure for InterPro Entry Q13188

    ProtoNet protein and cluster: Q13188

    UniProtKB/Swiss-Prot: STK3_HUMAN, Q13188
    Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily
    Similarity: Contains 1 protein kinase domain
    Similarity: Contains 1 SARAH domain


    STK3 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: STK3_HUMAN, Q13188
    Function: Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces
    chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling
    pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and
    promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in
    complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory
    protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of
    YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell
    proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of
    mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit
    tumor formation. Phosphorylates NKX2-1 (By similarity). Phosphorylates NEK2 and plays a role in centrosome
    disjunction by regulating the localization of NEK2 to centrosome, and its ability to phosphorylate CROCC and
    CEP250. In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its
    phosphorylation. Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1
    on 'Ser-259'. Phosphorylates MOBKL1A and RASSF2. Phosphorylates MOBKL1B on 'Thr-74'. Acts cooperatively with
    MOBKL1B to activate STK38
    Catalytic activity: ATP + a protein = ADP + a phosphoprotein
    Enzyme regulation: Inhibited by the C-terminal non-catalytic region. Activated by caspase-cleavage. Full
    activation also requires homodimerization and autophosphorylation of Thr-180, which are inhibited by the
    proto-oncogene product RAF1. Activated by RASSF1 which acts by preventing its dephosphorylation
    Induction: Activity increases during mitosis

         Genatlas biochemistry entry for STK3:
    serine threonine kinase 3

         Enzyme Numbers (IUBMB): EC 2.7.11.11 2 EC 2.7.112

         Gene Ontology (GO): Selected molecular function terms (see all 10):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000287magnesium ion binding IDA8566796
    GO:0004672protein kinase activity TAS10840030
    GO:0004674protein serine/threonine kinase activity IDA18328708
    GO:0004702receptor signaling protein serine/threonine kinase activity IBA--
    GO:0004713protein tyrosine kinase activity ----
         
    STK3 for ontologies           About GeneDecksing


    Phenotypes:
         3 GenomeRNAi human phenotypes for STK3:
     Decreased viability  Increased G1 DNA content  Paclitaxel antagonistic effect 

         Selected MGI mutant phenotypes (inferred from 6 alleles(MGI details for Stk3) (see all 18):
     behavior/neurological  cardiovascular system  cellular  digestive/alimentary  embryogenesis 
     growth/size/body  hematopoietic system  homeostasis/metabolism  immune system  integument 
     limbs/digits/tail  liver/biliary system  mortality/aging  nervous system  normal 

    STK3 for phenotypes           About GeneDecksing

    Animal Models:
         MGI mouse knock-outs for STK3: Stk3tm1.1Yy Stk3tm1Dlim Stk3tm1.1Jav

       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for STK3
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for STK3

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for STK3
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for STK3

    miRNA
    Products:
        
    miRTarBase miRNAs that target STK3:
    hsa-mir-192-5p (MIRT026447), hsa-mir-9-5p (MIRT021330), hsa-mir-215-5p (MIRT024880), hsa-mir-128-3p (MIRT021938), hsa-mir-141-3p (MIRT001813)

    Block miRNA regulation of human, mouse, rat STK3 using miScript Target Protectors
    8 qRT-PCR Assays for microRNAs that regulate STK3:
    hsa-miR-1290 hsa-miR-140-5p hsa-miR-205 hsa-miR-137 hsa-miR-511 hsa-miR-659 hsa-miR-9 hsa-miR-3121-3p
    SwitchGear 3'UTR luciferase reporter plasmidSTK3 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for STK3
    Predesigned siRNA for gene silencing in human, mouse, rat STK3

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for STK3

    Clone
    Products:
         
    OriGene clones in human, mouse for STK3 (see all 7)
    OriGene ORF clones in mouse, rat for STK3
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: STK3 (NM_006281)
    Sino Biological Human cDNA Clone for STK3
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for STK3
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat STK3

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for STK3
    Browse ESI BIO Cell Lines and PureStem Progenitors for STK3 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for STK3


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Subcellular locations from UniProtKB/Swiss-Prot
    STK3_HUMAN, Q13188: Cytoplasm (By similarity). Nucleus (By similarity). Note=The caspase-cleaved form cycles
    between nucleus and cytoplasm (By similarity). Phosphorylation at Thr-117 leads to inhibition of nuclear
    translocation
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    cytosol4
    nucleus2
    plasma membrane2
    cytoskeleton1

    Gene Ontology (GO): 3 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IEA--
    GO:0005737cytoplasm IDA19525978
    GO:0005829cytosol TAS--

    STK3 for ontologies           About GeneDecksing


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
    About This Section

    TryGeneCards Plus

    SuperPaths for STK3 About   (see all 7)                                                                                              See pathways by source

    SuperPathContained pathways About
    1MAPK signaling pathway
    MAPK signaling pathway0.50
    MAPK signaling pathway0.50
    2Signaling by GPCR
    Signal Transduction0.58
    3Sweet Taste Signaling
    Sperm Motility0.37
    4Signaling by Hippo
    Signaling by Hippo
    5Hippo signaling pathway
    Hippo signaling pathway

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    1 Downloadable PowerPoint Slide of GeneGlobe Pathway Central Maps for STK3
        Sperm Motility

    2 Cell Signaling Technology (CST) Pathways for STK3
        Apoptosis and Autophagy
    Cytoskeletal Signaling

    1 BioSystems Pathway for STK3
        MAPK signaling pathway


    1 Reactome Pathway for STK3
        Signaling by Hippo


    2 Kegg Pathways  (Kegg details for STK3):
        MAPK signaling pathway
    Hippo signaling pathway


    STK3 for pathways           About GeneDecksing

        Pathway & Disease-focused RT2 Profiler PCR Array including STK3: 
              Hedgehog Signaling Pathway in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for STK3

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for STK3 (Q131881, 2, 3 ENSP000003905004) via UniProtKB, MINT, STRING, and/or I2D (see all 260)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    HIST1H4AP628053I2D: score=4 
    HIST1H4BP628053I2D: score=4 
    HIST1H4CP628053I2D: score=4 
    HIST1H4DP628053I2D: score=4 
    HIST1H4EP628053I2D: score=4 
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 20):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001841neural tube formation ----
    GO:0003157endocardium development ----
    GO:0006468protein phosphorylation IDA--
    GO:0006915apoptotic process TAS11278283
    GO:0007165signal transduction TAS8816758

    STK3 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
    About This Section

    TryGeneCards Plus
    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for STK3

    3 HMDB Compounds for STK3    About this table
    CompoundSynonyms CAS #PubMed Ids
    ADPadenosindiphosphorsaeure (see all 8)58-64-0--
    Adenosine triphosphate5'-(tetrahydrogen triphosphate) Adenosine (see all 24)56-65-5--
    MagnesiumMagnesium (see all 2)7439-95-4--

    2 Novoseek inferred chemical compound relationships for STK3 gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    threonine 46.5 2 8566796 (1), 17379520 (1)
    serine 33.1 2 8566796 (1), 17379520 (1)



    STK3 for compounds           About GeneDecksing



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
    About This Section

    TryGeneCards Plus

    REFSEQ mRNAs for STK3 gene (3 alternative transcripts): 
    NM_001256312.1  NM_001256313.1  NM_006281.3  

    Unigene Cluster for STK3:

    Serine/threonine kinase 3
    Hs.492333  [show with all ESTs]
    Unigene Representative Sequence: NM_001256312
    17 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000517832 ENST00000419617(uc003yip.3 uc010mbm.1) ENST00000523601(uc003yio.3)
    ENST00000520440 ENST00000518582 ENST00000523159 ENST00000518165 ENST00000518627
    ENST00000522924 ENST00000521649 ENST00000520053 ENST00000521768 ENST00000520653
    ENST00000424861 ENST00000523567 ENST00000523960 ENST00000519420

    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat STK3 using miScript Target Protectors
    8 qRT-PCR Assays for microRNAs that regulate STK3:
    hsa-miR-1290 hsa-miR-140-5p hsa-miR-205 hsa-miR-137 hsa-miR-511 hsa-miR-659 hsa-miR-9 hsa-miR-3121-3p
    SwitchGear 3'UTR luciferase reporter plasmidSTK3 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for STK3
    Predesigned siRNA for gene silencing in human, mouse, rat STK3
    Clone
    Products:
         
    OriGene clones in human, mouse for STK3 (see all 7)
    OriGene ORF clones in mouse, rat for STK3
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: STK3 (NM_006281)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for STK3
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat STK3
    Primer
    Products:
        
    OriGene qPCR primer pairs and template standards for STK3
    OriGene qSTAR qPCR primer pairs in human, mouse for STK3
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat STK3
      QuantiTect SYBR Green Assays in human, mouse, rat STK3
      Search QuantiFast Probe-based Assays in human, mouse, rat STK3

    Additional mRNA sequence: 

    AK075229.1 AK131363.1 AK291837.1 AK307787.1 AK312660.1 BC010640.2 U26424.1 U60206.1 
    Z25422.1 

    10 DOTS entries:

    DT.215842  DT.100637314  DT.91721027  DT.121466825  DT.100754405  DT.102833033  DT.121466843  DT.86858028 
    DT.91875264  DT.92014795 

    Selected AceView cDNA sequences (see all 141):

    CD742983 AI284049 AA371498 BU186529 AU125963 BU689201 AW168289 AA370618 
    BM994152 BF035235 AK131363 BQ723525 AW300076 CB143850 AL546605 BU182450 
    BC010640 AA954682 BI964879 BP335254 AA370619 AL079539 R22038 CA391460 

    GeneLoc Exon Structure

    3 Alternative Splicing Database (ASD) splice patterns (SP) for STK3    About this scheme

    ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6a · 6b ^ 7a · 7b ^ 8a · 8b ^ 9 ^ 10 ^ 11
    SP1:                                -           -     -                                 
    SP2:                                            -                                       
    SP3:                                                                                    


    ECgene alternative splicing isoforms for STK3

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    TryGeneCards Plus

    STK3 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: TTCCTGATGA
    STK3 Expression
    About this image

    STK3 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    STK3 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.492333

    UniProtKB/Swiss-Prot: STK3_HUMAN, Q13188
    Tissue specificity: Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels
    in adult heart, lung and brain tissues

        Pathway & Disease-focused RT2 Profiler PCR Array including STK3: 
              Hedgehog Signaling Pathway in human mouse rat

    Primer
    Products:
    OriGene qPCR primer pairs and template standards for STK3
    OriGene qSTAR qPCR primer pairs in human, mouse for STK3
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat STK3
    QuantiTect SYBR Green Assays in human, mouse, rat STK3
    Search QuantiFast Probe-based Assays in human, mouse, rat STK3
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for STK3

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    TryGeneCards Plus

    This gene was present in the common ancestor of animals and fungi.

    Orthologs for STK3 gene from Selected species (see all 17)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Stk31 , 5 serine/threonine kinase 3 (Ste20, yeast homolog)5
    serine/threonine kinase 31
    90.06(n)1
    97.1(a)1
      15 (14.34 cM)5
    562741  NM_019635.21  NP_062609.21 
     348754965 
    chicken
    (Gallus gallus)
    Aves STK36
    Gallus gallus serine/threonine kinase 3 (STK3), mR...
    96(a)
    1 ↔ 1
    2(127436600-127569389)
    lizard
    (Anolis carolinensis)
    Reptilia STK36
    serine/threonine kinase 3
    95(a)
    1 ↔ 1
    4(20900284-21053699)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia stk31 serine/threonine kinase 3 81.44(n)
    94.41(a)
      100036637  NM_001097196.1  NP_001090665.1 
    zebrafish
    (Danio rerio)
    Actinopterygii wufc19e112 wufc19e11 77.8(n)   324125  BC048033.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta CG112283
    hpo1
    protein amino acid phosphorylation
    receptor signaling more3
    hippo1
    51(a)3
    63.43(n)1
    73.43(a)1
      56D133
    372471  NM_137583.51  NP_611427.11 
    worm
    (Caenorhabditis elegans)
    Secernentea C24A8.4a3
    cst-21
    G-protein coupled receptor3
    cst-21
    59(a)
    (best of 4)3
    66.9(n)1
    73.94(a)1
      X(4365362-4371318)3
    1807071  NM_001047754.31  NP_001041219.21 
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes SPS16
    Putative protein serine/threonine kinase expressed...
    28(a)
    1 → many
    IV(1485566-1487038) YDR523C


    ENSEMBL Gene Tree for STK3 (if available)
    TreeFam Gene Tree for STK3 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section

    TryGeneCards Plus
    Paralogs for STK3 gene
    STK242  STK42  STK252  MST42  
    Selected SIMAP similar genes for STK3 using alignment to 5 protein entries:     STK3_HUMAN (see all proteins) (see all similar genes):
    STK4/SLC36AL fusion    STK4    DKFZp686A2068    CDK3    CDKL2    STK25
    STK24    CDK1    DKFZp686J1430    MAP4K2    PAK4    TAOK3
    MYO3A    MST4    MYO3B    MAP4K4    MAP4K1    PAK3

    STK3 for paralogs           About GeneDecksing


    1 Pseudogenes.org Pseudogene for STK3
    PGOHUM00000233139


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
    About This Section

    TryGeneCards Plus

    Selected SNPs for STK3 (see all 7811)    About this table    
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 8 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    VAR_0411224
    An ovarian clear cell carcinoma sample4--see VAR_0411222 V L mis40--------
    rs1390739351,2
    C--95042839(+) CTCCCA/GGGCTC 2 -- us2k10--------
    rs1821899671,2
    --95042859(+) TCCCAC/TCTCTG 2 -- us2k10--------
    rs1859791761,2
    --95042894(+) TGCCCA/GGCTAC 2 -- us2k10--------
    rs343344991,2
    C--95056754(+) GTCTC-/A/AA  
            
    AAAAA
    1 -- int10--------
    rs1479479091,2
    C--99466405(+) ATACC-/TTCTGT 3 -- ds50010--------
    rs726666121,2
    C,F--99466428(+) TGAACT/GCATCA 3 -- ds50011Minor allele frequency- G:0.03NA 120
    rs1870553071,2
    --99466451(+) CTCTCC/TCCTCC 3 -- ds50010--------
    rs1506517431,2
    C--99466462(+) AGCTAC/TGTGGT 3 -- ds50010--------
    rs1148143481,2
    F--99466491(+) TCCCTC/TGAAGT 3 -- ds50011Minor allele frequency- T:0.01WA 118

    HapMap Linkage Disequilibrium report for STK3 (99413631 - 99663631 bp, first 250kb of STK3)

    Structural Variations
         Database of Genomic Variants (DGV) 7 variations for STK3:    About this table    
    Variant IDTypeSubtypePubMed ID
    esv2652228CNV Deletion19546169
    esv2737350CNV Deletion23290073
    nsv507477CNV Insertion20534489
    nsv831406CNV Loss17160897
    nsv891219CNV Loss21882294
    nsv891218CNV Loss21882294
    nsv891220CNV Gain21882294

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing STK3
    DNA2.0 Custom Variant and Variant Library Synthesis for STK3

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    TryGeneCards Plus
    OMIM gene information: 605030    OMIM disorders: --

    9 diseases for STK3:    About MalaCards
    soft tissue sarcoma    conjunctivitis    parkinson's disease    sarcoma
    squamous cell carcinoma    hepatocellular carcinoma    breast cancer    neuronitis
    malaria


    STK3 for disorders           About GeneDecksing


    Congresses - knowledge worth sharing:
    Alzheimer's & Parkinson's Diseases Congress (ADPD) 18 - 22 March 2015

    2 Novoseek inferred disease relationships for STK3 gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    warts 64.2 2 17538946 (1), 17379520 (1)
    tumors 33.4 7 17538946 (2), 20212043 (2), 17379520 (1), 15688006 (1)

    Genetic Association Database (GAD): STK3

    Export disorders for STK3 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    TryGeneCards Plus

    PubMed articles for STK3 gene, integrated from 10 sources (see all 74):
    (articles sorted by number of sources associating them with STK3)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Newly identified stress-responsive protein kinases, Krs-1 and Krs- 2. (PubMed id 8816758)1, 2, 3 Taylor L.K.... Erikson R.L. (Proc. Natl. Acad. Sci. U.S.A. 1996)
    2. Mammalian Ste20-like kinase (Mst2) indirectly supports Raf-1/ERK pathway activity via maintenance of protein phosphatase-2A catalytic subunit levels and consequent suppression of inhibitory Raf-1 phosphorylation. (PubMed id 20212043)1, 2, 9 Kilili G.K. and Kyriakis J.M. (J. Biol. Chem. 2010)
    3. Regulation of proapoptotic mammalian ste20-like kinase MST2 by the IGF1-Akt pathway. (PubMed id 20231902)1, 2, 9 Kim D.... Cheng J.Q. (PLoS ONE 2010)
    4. MOBKL1A/MOBKL1B phosphorylation by MST1 and MST2 inhibits cell proliferation. (PubMed id 18328708)1, 2, 9 Praskova M.... Avruch J. (Curr. Biol. 2008)
    5. Role of the kinase MST2 in suppression of apoptosis by the proto- oncogene product Raf-1. (PubMed id 15618521)1, 2, 9 O'Neill E.... Kolch W. (Science 2004)
    6. Cloning and characterization of a member of the MST subfamily of Ste20-like kinases. (PubMed id 8566796)1, 2, 9 Creasy C.L. and Chernoff J. (Gene 1995)
    7. The Ste20-like kinase Mst2 activates the human large tumor suppressor kinase Lats1. (PubMed id 15688006)1, 2, 9 Chan E.H.Y.... Sillje H.H.W. (Oncogene 2005)
    8. MST, a physiological caspase substrate, highly sensitizes apoptosis both upstream and downstream of caspase activation. (PubMed id 11278283)1, 2, 9 Lee K.-K.... Yonehara S. (J. Biol. Chem. 2001)
    9. Mammalian MST2 kinase and human Salvador activate and reduce estrogen receptor alpha in the absence of ligand. (PubMed id 21104395)1, 2 Park Y.... Lee Y. (J. Mol. Med. 2011)
    10. The tumor suppressor RASSF1A prevents dephosphorylation of the mammalian STE20-like kinases MST1 and MST2. (PubMed id 21199877)1, 2 Guo C.... Pfeifer G.P. (J. Biol. Chem. 2011)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

    TryGeneCards Plus
     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section

    TryGeneCards Plus
    Entrez Gene: 6788 HGNC: 11406 AceView: STK3 Ensembl:ENSG00000104375 euGenes: HUgn6788
    ECgene: STK3 Kegg: 6788 H-InvDB: STK3

    (According to HUGE)
    About This Section

    TryGeneCards Plus
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

    TryGeneCards Plus
    NameDescription
    PharmGKB entry for STK3 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for STK3 Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

    TryGeneCards Plus
    Patent Information for STK3 gene:
    Search GeneIP for patents involving STK3

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, and ESI BIO, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
    About This Section

    TryGeneCards Plus

     Browse Kits and Assays available from EMD Millipore
     EMD Millipore Purified and/or Recombinant STK3 Protein
     Browse Small Molecules at EMD Millipore
     EMD Millipore Mono- and Polyclonal Antibodies for the study of STK3
     
     EMD Millipore genomic analysis products

      
     Antibodies for STK3 (MST2/STK3)   Browse Cell Culture Products  
     Browse ELISAs   Browse Flow Cytometry Kits  
     Browse Primer Pairs   Browse Enzyme Activity Assays/Reagents  
     Browse ELISpot/FluoroSpot Kits/Development Modules   Browse TFB/Immunoprecipitation Assays  
     Browse Apoptosis Detection Kits/Reagents   Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents  
     Browse DNA Damage/Repair Kits/Reagents   Browse Luminex Assays  
     Browse Cell Selection/Detection Kits/Reagents   Browse Secondary Antibodies/Controls/Staining Reagents  
     Browse Recombinant/Natural Proteins   Browse Stem Cell Products  
     Browse cDNA Clones   Browse Proteome Profiler Antibody Arrays  
     OriGene Antibodies for STK3   OriGene RNAi products in human, mouse, rat for STK3  
     OriGene qPCR primer pairs and template standards for STK3   OriGene Protein Over-expression Lysate for STK3  
     OriGene MassSpec something-or-other for STK3   OriGene clones in human, mouse for STK3  
     OriGene qSTAR qPCR primer pairs in human, mouse for STK3   OriGene Purified Protein for STK3  
     OriGene ORF clones in mouse, rat for STK3   OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling  
     OriGene Custom Antibody Services for STK3   OriGene Custom Protein Services for STK3  

     
     
     Block miRNA regulation of human, mouse, rat STK3 using miScript Target Protectors SeqTarget long-range PCR primers for resequencing STK3
     DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat STK3 Predesigned siRNA for gene silencing in human, mouse, rat STK3
     Search QuantiFast Probe-based Assays in human, mouse, rat STK3 QuantiTect SYBR Green Assays in human, mouse, rat STK3
     PCR Arrays including human, mouse, rat STK3 Search Chromatin IP Primers for STK3
     Pre-validated RT2 qPCR Primer Assay in human, mouse, rat STK3  GeneGlobe Interaction Network for STK3
     Regulatory tfbs in STK3 promoter
     GenScript Custom Purified and Recombinant Proteins Services for STK3 GenScript cDNA clones with any tag delivered in your preferred vector for STK3
     GenScript Custom Assay Services for STK3 GenScript Custom overexpressing Cell Line Services for STK3
     CloneReady with Over 120,000 Genes  Gene Synthesis: Any Gene in Any Vector
     Vector-based siRNA and miRNA, Ready for Transfection Gene Mutant Library, Variants up to 10^11
     Plasmid Preparation Custom Peptide Services
     Antibodies & Assays for STK3  (MST2)

     Search Tocris compounds for STK3
     Browse Sino Biological Proteins
     Browse Sino Biological Cell Lysates
     cDNA Clones for STK3
     4000+ Proteins
     Search Sino Biological for antibodies, proteins & pathways
     Protein Production Services
     Transfection Reagents
     Protein A/G/L resins
     Isotyping reagents
     Proteins for STK3
     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

     Novus Tissue Slides
     STK3 antibodies
     STK3 proteins
     STK3 lysates
     Antibodies for STK3
     See all of Abcam's Antibodies, Kits and Proteins for STK3
     Custom Antibody / Protein Production Service
     Bulk Purchasing
     Advantages of Rabbit Monoclonal antibodies
     Abcam protocols and scientific support
     Browse ProSpec Recombinant Proteins
     Proteins for STK3
     Antibodies for STK3
     ELISAs for STK3
     CLIAs for STK3



     Browse ESI BIO Cell Lines and PureStem Progenitors for STK3
     Gene Synthesis
     Protein Engineering
     Variant Library Synthesis
     Codon Optimization
     Protein Production and Purification
     Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for STK3
     SwitchGear 3'UTR luciferase reporter plasmids for STK3
     SwitchGear Promoter luciferase reporter plasmids for STK3
     ThermoFisher Antibody for STK3
     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat STK3
     inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for STK3
     inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for STK3
     LSBio Antibodies in human, mouse, rat for STK3
    Customized transgenic rodents for:
     Humanization
     Biomarker expression
     Off-target effect monitoring
     Translational medicine
     Tissue-specific gene expresssion
     Time-controlled gene expresssion
     Browse compounds at ApexBio
           
    GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014

    View Random Gene

    Category
    (GIFtS: )
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 


    Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

    Hot genes      Disease genes      STK3 gene at Home site.
    Version: 3.12.136 22 July 2014
    hostname: 356980-web2.xennexinc.com index build: 126 solr: 1.4