Free for academic non-profit institutions. Other users need a Commercial license

Aliases for STK3 Gene

Aliases for STK3 Gene

  • Serine/Threonine Kinase 3 2 3 5
  • Serine/Threonine Kinase 3 (Ste20, Yeast Homolog) 2 3
  • Mammalian STE20-Like Protein Kinase 2 3 4
  • Serine/Threonine-Protein Kinase Krs-1 3 4
  • STE20-Like Kinase MST2 3 4
  • EC 2.7.11.1 4 61
  • MST-2 3 4
  • KRS1 3 4
  • MST2 3 4
  • Serine/Threonine Kinase 3 (STE20 Homolog, Yeast) 3
  • Serine/Threonine-Protein Kinase 3 3
  • KB-1458E12.1 3
  • EC 2.7.11 61

External Ids for STK3 Gene

Previous GeneCards Identifiers for STK3 Gene

  • GC08M098475
  • GC08M099540
  • GC08M099135
  • GC08M099423
  • GC08M099536
  • GC08M099466
  • GC08M094670
  • GC08M099413
  • GC08M098401

Summaries for STK3 Gene

Entrez Gene Summary for STK3 Gene

  • This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. The N-terminal portion is transported to the nucleus where it homodimerizes to form the active kinase which promotes the condensation of chromatin during apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

GeneCards Summary for STK3 Gene

STK3 (Serine/Threonine Kinase 3) is a Protein Coding gene. Among its related pathways are Apoptosis and Autophagy and Sweet Taste Signaling. GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is STK4.

UniProtKB/Swiss-Prot for STK3 Gene

  • Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1 (By similarity). Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosome, and its ability to phosphorylate CROCC and CEP250. In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on Ser-259. Phosphorylates MOBKL1A and RASSF2. Phosphorylates MOBKL1B on Thr-74. Acts cooperatively with MOBKL1B to activate STK38.

Gene Wiki entry for STK3 Gene

No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for STK3 Gene

Genomics for STK3 Gene

Regulatory Elements for STK3 Gene

Enhancers for STK3 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH08G098973 0.9 Ensembl ENCODE 12.3 -31.2 -31228 1.7 CTCF RNF2 SUZ12 CHD1 MAX CBX2 RBBP5 RAD21 FOXA1 ZBTB33 STK3 OSR2 VPS13B LOC105375666 GC08P098985 ENSG00000229625
GH08G098983 0.8 ENCODE 12.3 -40.7 -40661 0.2 PKNOX1 ZNF146 JUN BMI1 RAD21 ZBTB40 RFX5 CTBP1 GATA3 ARID3A STK3 OSR2 VPS13B LOC105375666 GC08P098985 ENSG00000229625
GH08G099002 0.7 Ensembl 11.1 -59.7 -59674 0.2 PKNOX1 USF1 SUZ12 NFIB MTA2 BHLHE40 USF2 BMI1 TBX21 SP1 STK3 OSR2 ENSG00000253180 GC08P098985
GH08G098997 0.6 ENCODE 10.9 -55.2 -55211 0.1 CTCF ZNF654 TRIM22 SMC3 REST RAD21 SPI1 OSR2 STK3 ENSG00000253562 ENSG00000253180 GC08P098985
GH08G098939 2 FANTOM5 Ensembl ENCODE dbSUPER 2.7 -2.7 -2660 12.9 PKNOX1 FOXA2 CREB3L1 ZFP64 ARID4B SIN3A DMAP1 ZBTB7B ZNF766 ZNF143 POP1 OSR2 STK3 ENSG00000271930 ENSG00000229625
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around STK3 on UCSC Golden Path with GeneCards custom track

Promoters for STK3 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00000227777 1271 601 FUS ARID4B SIN3A ZBTB7B RAD21 ELK1 EGR1 HSF1 ZNF143 ZNF207

Genomic Location for STK3 Gene

Chromosome:
8
Start:
98,371,355 bp from pter
End:
98,942,827 bp from pter
Size:
571,473 bases
Orientation:
Minus strand

Genomic View for STK3 Gene

Genes around STK3 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
STK3 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for STK3 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for STK3 Gene

Proteins for STK3 Gene

  • Protein details for STK3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q13188-STK3_HUMAN
    Recommended name:
    Serine/threonine-protein kinase 3
    Protein Accession:
    Q13188
    Secondary Accessions:
    • A8K722
    • B3KYA7
    • Q15445
    • Q15801
    • Q96FM6

    Protein attributes for STK3 Gene

    Size:
    491 amino acids
    Molecular mass:
    56301 Da
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    • Homodimer; mediated via the coiled-coil region. Interacts with NORE1, which inhibits autoactivation (By similarity). Interacts with and stabilizes SAV1. Interacts with RAF1, which prevents dimerization and phosphorylation. Interacts with RASSF1. Interacts (via SARAH domain) with isoform 1 of NEK2. Interacts with ESR1 only in the presence of SAV1. Interacts with PKB/AKT1. Forms a tripartite complex with MOBKL1B and STK38. Interacts with RASSF2 (via SARAH domain).

    Three dimensional structures from OCA and Proteopedia for STK3 Gene

    Alternative splice isoforms for STK3 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for STK3 Gene

Selected DME Specific Peptides for STK3 Gene

Q13188:
  • DIKAGNIL
  • HKESGQV
  • EMAEGKPPY
  • STMSEGAQTMIEHNSTMLESDLGTMVINS
  • RPSFMDYFDKQD
  • GTPFWMAP
  • YYGSYFK
  • LEKLGEG
  • IEELRQR
  • ATQLLQHPF
  • VAIKQVPVESD
  • ALDPMME
  • ADFGVAGQLTDT
  • KAGNILL
  • HRDIKAGN
  • KLGEGSYGSV
  • IMQQCDS
  • DFVKKCL
  • WMAPEVI
  • WKVPQDGD
  • GSVFKAI
  • DSHTMVK
  • IKAKRHEE
  • IHPMRAIFMIP

Post-translational modifications for STK3 Gene

  • Phosphorylation at Thr-117 and Thr-384 by PKB/AKT1, leads to inhibition of its: cleavage, kinase activity, autophosphorylation at Thr-180, binding to RASSF1 and nuclear translocation, and increase in its binding to RAF1.
  • Proteolytically cleaved by caspase-3 during apoptosis. Proteolytic cleavage results in kinase activation and nuclear translocation of the truncated form (MST1/N).
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for STK3 (MST2)
  • Santa Cruz Biotechnology (SCBT) Antibodies for STK3

Domains & Families for STK3 Gene

Gene Families for STK3 Gene

Graphical View of Domain Structure for InterPro Entry

Q13188

UniProtKB/Swiss-Prot:

STK3_HUMAN :
  • Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Family:
  • Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
genes like me logo Genes that share domains with STK3: view

Function for STK3 Gene

Molecular function for STK3 Gene

GENATLAS Biochemistry:
serine threonine kinase 3
UniProtKB/Swiss-Prot CatalyticActivity:
ATP + a protein = ADP + a phosphoprotein.
UniProtKB/Swiss-Prot EnzymeRegulation:
Inhibited by the C-terminal non-catalytic region. Activated by caspase-cleavage. Full activation also requires homodimerization and autophosphorylation of Thr-180, which are inhibited by the proto-oncogene product RAF1. Activated by RASSF1 which acts by preventing its dephosphorylation.
UniProtKB/Swiss-Prot Function:
Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1 (By similarity). Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosome, and its ability to phosphorylate CROCC and CEP250. In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on Ser-259. Phosphorylates MOBKL1A and RASSF2. Phosphorylates MOBKL1B on Thr-74. Acts cooperatively with MOBKL1B to activate STK38.
UniProtKB/Swiss-Prot Induction:
Activity increases during mitosis.

Enzyme Numbers (IUBMB) for STK3 Gene

Gene Ontology (GO) - Molecular Function for STK3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000287 magnesium ion binding IDA 8566796
GO:0004672 protein kinase activity IEA,IDA 28087714
GO:0004674 protein serine/threonine kinase activity IEA,IDA 8566796
GO:0004702 signal transducer, downstream of receptor, with serine/threonine kinase activity IBA --
GO:0005515 protein binding IPI 14729613
genes like me logo Genes that share ontologies with STK3: view
genes like me logo Genes that share phenotypes with STK3: view

Animal Models for STK3 Gene

MGI Knock Outs for STK3:

Animal Model Products

  • Taconic Biosciences Mouse Models for STK3

Inhibitory RNA Products

Flow Cytometry Products

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for STK3 Gene

Localization for STK3 Gene

Subcellular locations from UniProtKB/Swiss-Prot for STK3 Gene

Cytoplasm. Nucleus. Note=The caspase-cleaved form cycles between nucleus and cytoplasm (By similarity). Phosphorylation at Thr-117 leads to inhibition of nuclear translocation. {ECO:0000250, ECO:0000269 PubMed:19525978}.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for STK3 gene
Compartment Confidence
cytosol 5
nucleus 4
plasma membrane 2

Gene Ontology (GO) - Cellular Components for STK3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IEA --
GO:0005737 cytoplasm IEA,IDA 19525978
GO:0005829 cytosol TAS --
GO:0043234 protein complex IDA 19962960
genes like me logo Genes that share ontologies with STK3: view

Pathways & Interactions for STK3 Gene

genes like me logo Genes that share pathways with STK3: view

Pathways by source for STK3 Gene

2 Cell Signaling Technology pathways for STK3 Gene
1 BioSystems pathway for STK3 Gene
2 Reactome pathways for STK3 Gene

SIGNOR curated interactions for STK3 Gene

Activates:
Inactivates:
Is activated by:
Is inactivated by:

Gene Ontology (GO) - Biological Process for STK3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001841 neural tube formation IEA --
GO:0003157 endocardium development IEA --
GO:0006468 protein phosphorylation IDA,IEA 8566796
GO:0006915 apoptotic process TAS 11278283
GO:0007165 signal transduction IEA,TAS 8816758
genes like me logo Genes that share ontologies with STK3: view

Drugs & Compounds for STK3 Gene

(3) Drugs for STK3 Gene - From: HMDB and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Magnesium Nutra 0
genes like me logo Genes that share compounds with STK3: view

Transcripts for STK3 Gene

Unigene Clusters for STK3 Gene

Serine/threonine kinase 3:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for STK3 Gene

ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6a · 6b ^ 7a · 7b ^ 8a · 8b ^ 9 ^ 10 ^ 11
SP1: - - -
SP2: -
SP3:

Relevant External Links for STK3 Gene

GeneLoc Exon Structure for
STK3
ECgene alternative splicing isoforms for
STK3

Expression for STK3 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for STK3 Gene

Protein differential expression in normal tissues from HIPED for STK3 Gene

This gene is overexpressed in Lung (40.8).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for STK3 Gene



Protein tissue co-expression partners for STK3 Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of STK3 Gene:

STK3

SOURCE GeneReport for Unigene cluster for STK3 Gene:

Hs.492333

mRNA Expression by UniProt/SwissProt for STK3 Gene:

Q13188-STK3_HUMAN
Tissue specificity: Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues.

Evidence on tissue expression from TISSUES for STK3 Gene

  • Eye(4.2)
genes like me logo Genes that share expression patterns with STK3: view

Primer Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for STK3 Gene

Orthologs for STK3 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for STK3 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia STK3 34 35
  • 99.68 (n)
platypus
(Ornithorhynchus anatinus)
Mammalia STK3 35
  • 96 (a)
OneToOne
dog
(Canis familiaris)
Mammalia STK3 34 35
  • 94.82 (n)
cow
(Bos Taurus)
Mammalia STK3 34 35
  • 93.79 (n)
mouse
(Mus musculus)
Mammalia Stk3 34 16 35
  • 90.06 (n)
rat
(Rattus norvegicus)
Mammalia Stk3 34
  • 89.37 (n)
oppossum
(Monodelphis domestica)
Mammalia STK3 35
  • 89 (a)
OneToOne
chicken
(Gallus gallus)
Aves STK3 35
  • 96 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia STK3 35
  • 95 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia stk3 34
  • 81.44 (n)
zebrafish
(Danio rerio)
Actinopterygii stk3 34 35
  • 77.87 (n)
wufc19e11 34
fruit fly
(Drosophila melanogaster)
Insecta hpo 34 35
  • 63.43 (n)
CG11228 36
  • 51 (a)
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP006044 34
  • 59.43 (n)
worm
(Caenorhabditis elegans)
Secernentea cst-2 34 35
  • 66.9 (n)
C24A8.4a 36
  • 59 (a)
C24A8.4b 36
  • 59 (a)
F14H12.4a 36
  • 54 (a)
F14H12.4b 36
  • 54 (a)
cst-1 35
  • 53 (a)
ManyToMany
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes SPS1 35
  • 28 (a)
OneToMany
sea squirt
(Ciona savignyi)
Ascidiacea CSA.8767 35
  • 69 (a)
OneToMany
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.1634 34
Species where no ortholog for STK3 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for STK3 Gene

ENSEMBL:
Gene Tree for STK3 (if available)
TreeFam:
Gene Tree for STK3 (if available)

Paralogs for STK3 Gene

Variants for STK3 Gene

Sequence variations from dbSNP and Humsavar for STK3 Gene

SNP ID Clin Chr 08 pos Sequence Context AA Info Type
VAR_041122 An ovarian clear cell carcinoma sample
rs1000003045 -- 98,835,825(+) CAGCC(C/T)CAGGG intron-variant
rs1000006679 -- 98,831,879(+) TGAAC(A/C)TCTTG intron-variant
rs1000007005 -- 98,873,981(+) AGTAC(C/T)GGTTT intron-variant
rs1000011098 -- 98,546,297(+) GATGT(C/T)GGAAA intron-variant

Structural Variations from Database of Genomic Variants (DGV) for STK3 Gene

Variant ID Type Subtype PubMed ID
esv24438 CNV loss 19812545
esv2652228 CNV deletion 19546169
esv2737350 CNV deletion 23290073
esv2737352 CNV deletion 23290073
esv3572918 CNV loss 25503493
esv3618155 CNV loss 21293372
esv3618156 CNV loss 21293372
esv3618157 CNV loss 21293372
esv3891446 CNV gain 25118596
nsv1030498 CNV loss 25217958
nsv1150495 CNV duplication 26484159
nsv507477 OTHER sequence alteration 20534489
nsv515049 CNV gain 21397061
nsv611808 CNV loss 21841781
nsv611809 CNV loss 21841781
nsv6327 CNV deletion 18451855
nsv831406 CNV loss 17160897
nsv981968 CNV duplication 23825009

Variation tolerance for STK3 Gene

Residual Variation Intolerance Score: 26.1% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 1.26; 25.14% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for STK3 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
STK3

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for STK3 Gene

Disorders for STK3 Gene

Relevant External Links for STK3

Genetic Association Database (GAD)
STK3
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
STK3

No disorders were found for STK3 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for STK3 Gene

Publications for STK3 Gene

  1. Mammalian Ste20-like kinase (Mst2) indirectly supports Raf-1/ERK pathway activity via maintenance of protein phosphatase-2A catalytic subunit levels and consequent suppression of inhibitory Raf-1 phosphorylation. (PMID: 20212043) Kilili G.K. … Kyriakis J.M. (J. Biol. Chem. 2010) 3 4 22 64
  2. Regulation of proapoptotic mammalian ste20-like kinase MST2 by the IGF1-Akt pathway. (PMID: 20231902) Kim D. … Cheng J.Q. (PLoS ONE 2010) 3 4 22 64
  3. MOBKL1A/MOBKL1B phosphorylation by MST1 and MST2 inhibits cell proliferation. (PMID: 18328708) Praskova M. … Avruch J. (Curr. Biol. 2008) 3 4 22 64
  4. The Ste20-like kinase Mst2 activates the human large tumor suppressor kinase Lats1. (PMID: 15688006) Chan E.H.Y. … Sillje H.H.W. (Oncogene 2005) 3 4 22 64
  5. Role of the kinase MST2 in suppression of apoptosis by the proto- oncogene product Raf-1. (PMID: 15618521) O'Neill E. … Kolch W. (Science 2004) 3 4 22 64

Products for STK3 Gene

Sources for STK3 Gene

Content
Loading form....