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ST5 Gene

protein-coding   GIFtS: 57

GC11M008671
suppression of tumorigenicity 5
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
DENND2B 1, 2, 3
HTS1 1, 2, 3, 5
MGC33090 2
p126 1, 2
Descriptions
DENN domain-containing protein 2B 3
DENN/MADD domain containing 2B 2
HeLa tumor suppression 1 3
suppression of tumorigenicity 5 2
External Ids
HGNC: 113501
Entrez Gene: 67642
UniProtKB: P785243
Ensembl: ENSG000001664447
Search outside databases for aliases for ST5 gene

Previous GC identifers: GC11M009485 GC11M009161 GC11M008754 GC11M008679

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for ST5:
This gene was identified by its ability to suppress the tumorigenicity of Hela cells in nude mice.
The protein encoded by this gene contains a C-terminal region that shares similarity with the Rab
3 family of small GTP binding proteins. This protein preferentially binds to the SH3 domain of
c-Abl kinase, and acts as a regulator of MAPK1/ERK2 kinase, which may contribute to its ability to
reduce the tumorigenic phenotype in cells. Three alternatively spliced transcript variants of this
gene encoding distinct isoforms are identified. [provided by RefSeq]

UniProtKB/Swiss-Prot: ST5_HUMAN, P78524
Function: May be involved in cytoskeletal organization and tumorgenicity. Isoform 1 seems to be
involved in a signaling transduction pathway leading to activation of MAPK1/ERK2. Isoform 3 may
block ERK2 activation stimulated by ABL1. Isoform 3 may alter cell morphology and cell growth

Gene Wiki entry for ST5

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the ST5 gene  

Entrez Gene cytogenetic band: 11p15   Ensembl cytogenetic band:  11p15.4   HGNC cytogenetic band: 11p15

ST5 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 11         GeneLoc Exon Structure

GeneLoc location for GC11M008671:     (about GC identifiers)

Start:
8,671,475 bp from pter
End:
8,889,074 bp from pter
Size:
217,600 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000011.8  NT_009237.17  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: ST5_HUMAN, P78524 (See protein sequence)
Recommended Name: Suppression of tumorigenicity 5  
Size: 1137 amino acids; 126499 Da
Subunit: Isoform 1 interacts with the SH3 domain of ABL1
Secondary accessions: B3KXQ6 P78523 Q16492 Q7KYY2 Q7KZ12 Q8NE12 Q9BQQ6
Alternative promoter usage, Alternative splicing: 3 isoforms:  P78524-1   P78524-2   P78524-3   

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins (3 alternative transcripts): 
    NP_005409.3  NP_631896.1  NP_998783.1  


    ENSEMBL proteins: 
    ENSP00000299567 ENSP00000319678 ENSP00000350294 ENSP00000379900 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (ST5)
    Human Recombinant Proteins from Abnova (ST5)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 


    Antibodies for ST5: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for ST5
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (ST5), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (ST5)
    Novus Biologicals Antibodies for ST5

    Assays for ST5: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    3 InterPro domains/families:
     IPR005112 dDENN
     IPR005113 uDENN
     IPR001194 DENN


       GeneDecks  ST5 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry P78524

    ProtoNet protein and cluster: P78524

    UniProtKB/Swiss-Prot: ST5_HUMAN, P78524
    Similarity: Contains 1 dDENN domain
    Similarity: Contains 1 DENN domain
    Similarity: Contains 1 uDENN domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (ST5)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (ST5)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NM_139157

                  Applied Biosystems Silencer® siRNAs for ST5

                  Sigma-Aldrich siRNA for ST5  
                         Sigma-Aldrich shRNA Panels and shRNA for ST5  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 3): NM_005418
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 3): NM_005418
                                     untagged cDNA clones in CMV expression vector (see all 3): NM_005418 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_005418

    UniProtKB/Swiss-Prot: ST5_HUMAN, P78524
    Function: May be involved in cytoskeletal organization and tumorgenicity. Isoform 1 seems to be
    involved in a signaling transduction pathway leading to activation of MAPK1/ERK2. Isoform 3 may
    block ERK2 activation stimulated by ABL1. Isoform 3 may alter cell morphology and cell growth

    1 Gene Ontology (GO) molecular function term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515 protein binding IPI9632734
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


     Gene Network CentralTM Interacting Genes and Proteins Network for  ST5 


    5/15 Interacting proteins for ST5 (ENSP000003502943 P785241, 2) via UniProtKB, MINT, and/or STRING (see all 15 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    ABL1P005191EBI-962633, EBI-375543
    GRB2P629931EBI-962633, EBI-401755
    SFNP319472MINT-3975647
    SMAD2Q157962MINT-61618
    SDCBPENSP000002601303STRING (score=.952)
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for ST5

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (ST5)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (ST5)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NM_139157

                  Sigma-Aldrich siRNA for ST5  
                         Sigma-Aldrich shRNA Panels and shRNA for ST5  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_005418  NM_139157  NM_213618  

    REFSEQ mRNAs for ST5 gene (3 alternative transcripts): 

    NM_005418.3   NM_139157.2   NM_213618.1   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_005418  NM_139157  NM_213618  

                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 3): NM_005418
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 3): NM_005418
                                     untagged cDNA clones in CMV expression vector (see all 3): NM_005418 

    Additional cDNA sequence: 

    AK123380.1 AK126554.1 AK127763.1 AK293245.1 AK302824.1 AK307617.1 AK309929.1 AK312305.1 
    AK312758.1 BC036655.1 BX647431.1 BX647474.1 CR611680.1 S45936.1 U15131.1 U15779.1 
    U15780.1 

    24/38 DOTS entries (see all 38 ):

    DT.91832935  DT.100785453  DT.101981184  DT.455736  DT.92438514  DT.100785460  DT.75188964  DT.100785456 
    DT.120726121  DT.99977160  DT.95148226  DT.120726016  DT.120726172  DT.97795613  DT.95148234  DT.100785461 
    DT.92039293  DT.120726144  DT.120726150  DT.100739170  DT.86853926  DT.92438516  DT.100785458  DT.120725943 

    24/301 AceView cDNA sequences (see all 301 ):

    BU528932 CB529540 BU155307 AI269509 AA399294 BU070318 BM823967 BQ720478 
    CF454471 AI270279 AW082200 BU170512 BM716690 AL043546 BE048030 BF724958 
    BU783990 BE045970 BM972253 CR611680 BU193497 NM_213618 BX472075 CA441929 

    highest scoring ESTs for ST5:

    AK126554 U15131 U15780 BC036655 BG773425 AA029062 AA029166 AA044178 AA044363 AA131596 

    Unigene Cluster for ST5:

    Suppression of tumorigenicity 5
    Hs.117715  [show with all ESTs]
    Unigene Representative Sequence: NM_005418


    GeneLoc Exon Structure

    5/30 Alternative Splicing Database (ASD) splice patterns (SP) for ST5 (see all 30 )

    ExUns: 1 ^ 2a · 2b ^ 3 ^ 4 ^ 5 ^ 6a · 6b · 6c · 6d · 6e ^ 7 ^ 8 ^ 9 ^ 10a · 10b ^ 11 ^ 12 ^ 13 ^ 14a · 14b · 14c · 14d · 14e · 14f · 14g ·
    SP1:        -     -     -     -           -     -     -     -     -     -     -     -     -     -     -     -                                                   
    SP2:                                                                          -     -     -     -     -     -                                                   
    SP3:                                      -     -     -     -     -     -     -     -     -     -     -     -           -     -     -     -     -     -     -   
    SP4:                                                                                                                                                            
    SP5:                                                                                                                                                            

    ExUns: 14h · 14i · 14j ^ 15a · 15b · 15c ^ 16a · 16b ^ 17 ^ 18 ^ 19a · 19b · 19c ^ 20a · 20b ^ 21 ^ 22 ^ 23a · 23b · 23c ^ 24 ^ 25a · 25b ^ 26 ^ 27 ^ 28 ^
    SP1:                                                  -     -     -                                                                 -           -               
    SP2:                                                  -     -     -                                                                 -           -               
    SP3:  -     -     -     -     -     -                 -     -     -                                                                 -           -               
    SP4:                                                        -     -                                                                 -           -               
    SP5:                                                                                                                                                            

    ExUns: 29a · 29b ^ 30 ^ 31a · 31b ^ 32a · 32b ^ 33 ^ 34a · 34b ^ 35a · 35b · 35c ^ 36 ^ 37a · 37b
    SP1:              -                 -           -     -                                             
    SP2:              -                 -           -     -                                             
    SP3:              -                 -           -     -                                             
    SP4:              -                 -           -     -                                             
    SP5:                                -           -     -                                             

    About this scheme

    ECgene alternative splicing isoforms for ST5

    4 Ensembl transcripts including schematic representations:
    ENST00000353815  ENST00000313726  ENST00000357665  ENST00000396665  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    ST5 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for ST5

    1 / 2 / 3

    4 probe-sets matching ST5 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    37745_s_at2, 3 U95-A 1 1.00 1.00 0.94 0.78 U15780 0.80 1.00 0.91 1

    37746_r_at2, 3 U95-A 1 1.00 1.00 0.17 1.22 U15131 0.80 1.00 0.91 1

    202440_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_005418 0.60 1.00 0.82 1

    202440_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GGAAGCCCAC

    SOURCE GeneReport for Unigene cluster: Hs.117715

    Expression variation in blood from EXPOLDB for ST5

    UniProtKB/Swiss-Prot: ST5_HUMAN, P78524
    Tissue specificity: Widely expressed with the exception of peripheral blood lymphocytes. Isoform 1
    is expressed in several epithelial and fibroblast (including tumorigenic) but absent in lymphoid
    cell lines (at protein level). Isoform 3 is expressed in primary cell or weakly tumorigenic but
    not in tumorigenic cell lines (at protein level)

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for ST5 gene from 5/9 species (see all 9 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    ST51   -- suppression of tumorigenicity 5 92.84(n)
    94.96(a)
    485380  XM_542498.2  XP_542498.2 
    chimpanzee
    (Pan troglodytes)
    ST51   -- suppression of tumorigenicity 5 97.13(n)
    96.39(a)
    451727  XM_001168255.1  XP_001168255.1 
    cow
    (Bos taurus)
    ST51   -- suppression of tumorigenicity 5 92.05(n)
    93.7(a)
    537907  XM_618095.3  XP_618095.3 
    rat
    (Rattus norvegicus)
    St51   -- suppression of tumorigenicity 5 89.86(n)
    92.86(a)
    308944  XM_219273.4  XP_219273.3 
    mouse
    (Mus musculus)
    St51, 5 7 (51.55 cM)5
    suppression of tumorigenicity 51, 5 89.95(n)1
    93.3(a)1
    769541  NM_001001326.11  NP_001001326.11 
     AJ3076705  AK0081005  (see all 11)
    About this table        Species with no ortholog for ST5

    ENSEMBL Gene Tree for ST5
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for ST5 gene
    DENND2D2  DENND2A2  DENND2C2  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/1149 NCBI SNPs in ST5 are shown (see all 1149 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 360)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 11 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs39158681,2
    A,C,F,H8790376(-) GATTCT/CCCACC 3 -- int1 ng314Minor allele frequency- C:0.17EU EA WA 418
    rs37941531,2
    A,C,F,H8708465(-) AGGAAG/CACTGG 3 N/K mis1 int1 trp312Minor allele frequency- C:0.38EA NA EU WA 2408
    --
    rs64845471,2
    A,C,F,H8790976(+) CAGTTG/AATACC 2 -- ng31 int14Minor allele frequency- A:0.06EU EA WA 416
    rs116038411,2
    C,F,H8791256(+) TTTTTT/CCATTA 2 -- ng31 int17Minor allele frequency- C:0.41NA EU EA WA 506
    rs10506551,2
    A,C,H8671580(-) GCTCTC/TTGGGC 3 -- ut31 ese35Minor allele frequency- T:0.00MN EU EA WA 602
    rs38127621,2
    C,F,H8708216(+) TTCTTG/CTCAGC 3 E/D mis1 int1 ese317Minor allele frequency- C:0.28EA NA EU WA 2754
    rs38509361,2
    C,H8789602(-) CCCACG/TAAGCC 3 -- ng31 int13Minor allele frequency- T:0.00EU EA 300
    rs110420471,2
    C,H8692138(+) CATCAG/AAGTCG 3 F/S mis1 ese34Minor allele frequency- A:0.00EU EA WA 418
    --
    rs107430911,2
    A,C8789935(+) cactaC/Tatcat 3 -- ng31 int10--------
    rs110420811,2
    F,H8790586(+) GATTGG/ACATTA 3 -- ng31 int14Minor allele frequency- A:0.01EU EA WA 418
    About this table

    HapMap Linkage Disequilibrium images for ST5 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 140750

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/13 PubMed articles for ST5 gene (see all 13 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 6764 HGNC: 11350 AceView: ST5 Ensembl:ENSG00000166444 euGenes: HUgn6764
    ECgene: ST5 H-InvDB: ST5
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    ATLAS Chromosomes in Cancer entry for ST5 Genetics and Cytogenetics in Oncology and Haematology
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for ST5:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
      Invitrogen Clones  Invitrogen Q-PCR Products
      Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for ST5-related products

     Millipore Custom Antibody & Bulk Services
     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for ST5 Antibodies for ST5
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for ST5 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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