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SSX2 Gene

protein-coding   GIFtS: 54

GC0XM052742
synovial sarcoma, X breakpoint 2
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: SSX)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
CT5.2 3
CT5.2a 1, 2
HD21 1, 2
HOM-MEL-40 1, 2, 3
MGC119055 1, 2
MGC15364 1, 2
MGC3884 1, 2
OTTHUMP00000024290 2
OTTHUMP00000024291 2
SSX 2, 3
SSX2A 3
Descriptions
Cancer/testis antigen 5.2 3
cancer/testis antigen family 5, member 2a 2
sarcoma, synovial, X-chromosome-related 2 2
synovial sarcoma, X breakpoint 2 2, 3
synovial sarcoma, X breakpoint 2, isoform b 2
synovial sarcoma, X breakpoint 2B 2
External Ids
HGNC: 113361
Entrez Gene: 67572
UniProtKB: Q163853
Ensembl: ENSG000001877547
Search outside databases for aliases for SSX2 gene

Previous GC identifers: GC0XP043594 GC0XP050491 GC0XM051377 GC0XM051692 GC0XM052608

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for SSX2:
The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX)
breakpoint proteins. These proteins may function as transcriptional repressors. They are also
capable of eliciting spontaneously humoral and cellular immune responses in cancer patients, and
are potentially useful targets in cancer vaccine-based immunotherapy. SSX1, SSX2 and SSX4 genes
have been involved in the t(X;18) translocation characteristically found in all synovial sarcomas.
This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome
18 to one of the SSX genes on chromosome X. The encoded hybrid proteins are probably responsible
for transforming activity. Two transcript variants encoding distinct isoforms have been identified
for this gene. [provided by RefSeq]

UniProtKB/Swiss-Prot: SSX2_HUMAN, Q16385
Function: Could act as a modulator of transcription

Gene Wiki entry for SSX2

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the SSX2 gene  

Entrez Gene cytogenetic band: Xp11.22   Ensembl cytogenetic band:  Xp11.22   HGNC cytogenetic band: Xp11.22

SSX2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome X         GeneLoc Exon Structure

GeneLoc location for GC0XM052742:     (about GC identifiers)

Start:
52,742,671 bp from pter
End:
52,752,974 bp from pter
Size:
10,304 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000023.9  NT_011630.14  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: SSX2_HUMAN, Q16385 (See protein sequence)
Recommended Name: Protein SSX2  
Size: 188 amino acids; 21620 Da
Subunit: Interacts via its N-terminal region with RAB3IP and SSX2IP
Subcellular location: Nucleus
Secondary accessions: Q16404 Q5JS26 Q96IP7 Q9BU88
Alternative splicing: 2 isoforms:  Q16385-1   Q16385-2   

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins (2 alternative transcripts): 
    NP_003138.3  NP_783629.1  


    ENSEMBL proteins: 
    ENSP00000338796 ENSP00000338561 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (SSX2)
    Human Recombinant Proteins from Abnova (SSX2)
                   OriGene Purified Recombinant Human Proteins (see all 2): SSX2  


    Antibodies for SSX2: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for SSX2
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (SSX2), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (SSX2)
    Novus Biologicals Antibodies for SSX2

    Assays for SSX2: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    3 InterPro domains/families:
     IPR003655 Krueppel-associated_box-rel
     IPR019041 SSXRD_motif
     IPR001909 Krueppel-associated_box


       GeneDecks  SSX2 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q16385

    ProtoNet protein and cluster: Q16385

    1 Blocks protein family: IPB001909 KRAB box

    UniProtKB/Swiss-Prot: SSX2_HUMAN, Q16385
    Similarity: Belongs to the SSX family
    Similarity: Contains 1 KRAB-related domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (SSX2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (SSX2)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_003147

                  Applied Biosystems Silencer® siRNAs for SSX2

                  Sigma-Aldrich siRNA for SSX2  
                         Sigma-Aldrich shRNA for SSX2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for SSX2
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_003147
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_003147
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_003147 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_003147

    UniProtKB/Swiss-Prot: SSX2_HUMAN, Q16385
    Function: Could act as a modulator of transcription

    Genatlas biochemistry entry for SSX2:
    protein SSX2 with homology to Kruppel associated box,KRAB,transcriptional corepressor,complexing
    with polycomb group (PcG) proteins RING1 or BMI1 and associating with chromatin,fused with SYT
    (SSXT) in synovial sarcoma with translocation t(X;18)(p11.2;q11.2) (see BSSX)
    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


     Gene Network CentralTM Interacting Genes and Proteins Network for  SSX2 


    2 Interacting proteins for SSX2 (ENSP000003387963 Q163851) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    SSX2IPQ9Y2D81STRING (score=.938) EBI-2210673, EBI-2212028
    RAB3IPQ96QF0-21EBI-2210673, EBI-747865
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for SSX2
    2 Novoseek chemical compound relationships for SSX2 gene
    Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    oligonucleotide 0.00 1 15747098 (1)
    zinc 0.00 1 7539744 (1)
    About this table


    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (SSX2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (SSX2)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_003147

                  Sigma-Aldrich siRNA for SSX2  
                         Sigma-Aldrich shRNA for SSX2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_003147  NM_175698  

    REFSEQ mRNAs for SSX2 gene (2 alternative transcripts): 

    NM_003147.4   NM_175698.1   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_003147  NM_175698  

                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_003147
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_003147
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_003147 

    Additional cDNA sequence: 

    AF190791.1 BC002818.2 BC007343.2 BC016957.1 BC069313.1 BC071827.1 BC103863.2 S79332.1 
    Z49105.1 Z49106.1 

    8 DOTS entries:

    DT.203018  DT.121314213  DT.92450034  DT.95157176  DT.113859  DT.121278319  DT.121278317  DT.121314214 

    24/41 AceView cDNA sequences (see all 41 ):

    BE378730 CB158928 AF190791 NM_003147 X86175 CB156144 BC002818 CB158955 
    NM_175698 BC071827 BQ231752 BG396977 BU194624 BC007343 BE384545 BQ222907 
    Z49105 CB144604 BC016957 BC069313 BG104299 BE408978 BI520165 BU161779 

    highest scoring ESTs for SSX2:

    AL040237 BC002818 BC007343 BC016957 BC071827 BE391023 BE408978 BE891434 BG104299 BG396977 

    Unigene Clusters for SSX2:

    Synovial sarcoma, X breakpoint 2
    Hs.289105  [show with all ESTs], Hs.661107  [show with all ESTs]
    Unigene Representative Sequences: XM_001720499, NM_003147


    GeneLoc Exon Structure

    2 Ensembl transcripts including schematic representations:
    ENST00000336777  ENST00000337502  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    SSX2 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for SSX2

    1 / 2 / 3

    14 probe-sets matching SSX2 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    34069_s_at2, 3 U95-A 2 1.00 0.50 1.00 1.00 S79325 0.40 0.38 0.39 2

    36409_f_at2, 3 U95-A 12 1.00 0.30 -0.11 0.20 Z49105 0.80 0.88 0.84 1

    126_s_at2, 3 U95-A 12 1.00 0.24 -0.32 0.40 X79200 0.60 0.83 0.73 1

    33655_f_at2, 3 U95-A 12 0.94 0.17 -0.07 0.38 U90840 0.20 0.07 0.15 3

    216471_x_at2, 3 U133-A 16 1.00 0.24 -- -- X79200 0.60 0.83 0.73 1

    210497_x_at2, 3 U133-A 12 1.00 0.16 -- -- BC002818 0.80 1.00 0.91 1

    207493_x_at2, 3 U133-A 19 1.00 0.15 -- -- NM_003147 0.60 1.00 0.82 1

    207666_x_at2 U133-A 12 0.82 0.15 -- -- -- -- -- -- --

    215881_x_at2 U133-A 21 0.73 0.17 -- -- -- -- -- -- --

    216471_x_at2 U133Plus2 16 1.00 0.24 -- -- -- -- -- -- --

    210497_x_at2 U133Plus2 12 1.00 0.16 -- -- -- -- -- -- --

    207493_x_at2 U133Plus2 19 1.00 0.15 -- -- -- -- -- -- --

    207666_x_at2 U133Plus2 12 0.82 0.15 -- -- -- -- -- -- --

    215881_x_at2 U133Plus2 21 0.73 0.17 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TACTCCCCTC

    SOURCE GeneReport for Unigene clusters: Hs.289105 Hs.661107

    Expression variation in blood from EXPOLDB for SSX2

    UniProtKB/Swiss-Prot: SSX2_HUMAN, Q16385
    Tissue specificity: Expressed at high level in the testis. Expressed at low level in thyroid. Not
    detected in tonsil, colon, lung, spleen, prostate, kidney, striated and smooth muscles. Detected
    in rhabdomyosarcoma and fibrosarcoma cell lines. Not detected in mesenchymal and epithelial cell
    lines

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for SSX2 gene from 1 species
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    chimpanzee
    (Pan troglodytes)
    SSX21   -- synovial sarcoma, X breakpoint 2 98.93(n)
    98.72(a)
    741661  XM_001143910.1  XP_001143910.1 
    About this table        Species with no ortholog for SSX2

    ENSEMBL Gene Tree for SSX2
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for SSX2 gene
    SSX2B1 2  SSX31 2  SSX62  SSX42  SSX72  SSX12  SSX52  SSX102  
    SSX92  SSX4B2  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section

      --
    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 300192

    UniProtKB/Swiss-Prot: SSX2_HUMAN, Q16385

  • A chromosomal aberration involving SSX2 may be a cause of synovial sarcoma. Translocation
    t(X;18)(p11.2;q11.2). The translocation is specifically found in more than 80% of synovial
    sarcoma. The fusion products SSXT-SSX1 or SSXT-SSX2 are probably responsible for transforming
    activity. Heterogeneity in the position of the breakpoint can occur (low frequency)
  • 10/11 Novoseek disease relationships for SSX2 gene (see all 11 )

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    sarcoma synovial 95.86 40 10398111 (2), 7951320 (2), 8840996 (2), 15147271 (2) (see all 35)
    testicular cancer 77.66 12 10749136 (2), 14611804 (2), 16224274 (1), 11823502 (1) (see all 9)
    sarcoma 65.11 4 10398111 (1), 12601173 (1), 9788446 (1), 12469233 (1)
    melanoma 49.65 13 10749136 (4), 9378559 (2), 9639388 (2), 12007189 (1) (see all 5)
    tumors 46.92 27 9639388 (3), 18295890 (2), 14611804 (2), 11668506 (1) (see all 18)
    seminoma 44.83 3 11454979 (1), 9639388 (1)
    metastatic melanoma 27.14 1 10749136 (1)
    cancer 25.27 11 9378559 (3), 15603546 (3), 17186289 (1), 15596411 (1) (see all 6)
    metastasis 19.15 1 15781633 (1)
    ovarian cancer 14.98 2 9639388 (1)
    About this table

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/82 PubMed articles for SSX2 gene (see all 82 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 6757 HGNC: 11336 AceView: SSX2 Ensembl:ENSG00000187754 euGenes: HUgn6757
    ECgene: SSX2 H-InvDB: SSX2
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    ATLAS Chromosomes in Cancer entry for SSX2 Genetics and Cytogenetics in Oncology and Haematology
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for SSX2:
     TaqMan ® Gene Expression Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
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     Millipore Immunoassay Services
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     Predesigned and custom siRNAs for SSX2 Antibodies for SSX2
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for SSX2 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Recombinant Proteins
    (SSX2)
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     Tagged/untagged cDNA clones
     Validated SYBR primer pairs
     Purified proteins from HEK293T cells
     Western blot validated antibodies
     shRNA in GFP-retroviral vector
     Search Tocris compounds for SSX2




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for SSX2

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