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SSBP4 Gene

protein-coding   GIFtS: 49
GCID: GC19P018644

Single Stranded DNA Binding Protein 4

  Search for SSBP4
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Single Stranded DNA Binding Protein 41 2
Single-Stranded DNA-Binding Protein 42

External Ids:    HGNC: 156761   Entrez Gene: 1704632   Ensembl: ENSG000001305117   OMIM: 6073915   UniProtKB: Q9BWG43   

Export aliases for SSBP4 gene to outside databases

Previous GC identifers: GC19P019023 GC19P018922 GC19P018375 GC19P018391 GC19P018531 GC19P018093


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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GeneCards Summary for SSBP4 Gene:
SSBP4 (single stranded DNA binding protein 4) is a protein-coding gene. GO annotations related to this gene include single-stranded DNA binding. An important paralog of this gene is SSBP2.


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000019.9  NT_011295.12  NC_018930.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the SSBP4 gene promoter:
         RFX1   Sp1   p300   Olf-1   AP-4   Egr-4   HOXA5   STAT3   FOXO1a   FOXO1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidSSBP4 promoter sequence
   Search Chromatin IP Primers for SSBP4

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat SSBP4


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 19p13.1   Ensembl cytogenetic band:  19p13.11   HGNC cytogenetic band: 19p13.1

SSBP4 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
SSBP4 gene location

GeneLoc information about chromosome 19         GeneLoc Exon Structure

GeneLoc location for GC19P018644:  view genomic region     (about GC identifiers)

Start:
18,529,674 bp from pter      End:
18,545,372 bp from pter
Size:
15,699 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: SSBP4_HUMAN, Q9BWG4 (See protein sequence)
Recommended Name: Single-stranded DNA-binding protein 4  
Size: 385 amino acids; 39388 Da

Explore the universe of human proteins at neXtProt for SSBP4: NX_Q9BWG4

Explore proteomics data for SSBP4 at MOPED

Post-translational modifications: 

  • Modification sites at neXtProt
  • Modification sites at PhosphoSitePlus

  • See SSBP4 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (2 alternative transcripts): 
    NP_001009998.1  NP_116016.1  

    ENSEMBL proteins: 
     ENSP00000475538   ENSP00000252807   ENSP00000270061   ENSP00000475668   ENSP00000476092  
     ENSP00000472975   ENSP00000476084   ENSP00000475352   ENSP00000476567  

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    LSBio Antibodies in human, mouse, rat for SSBP4

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    Search eBioscience for ELISAs for SSBP4 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    2 InterPro protein domains:
     IPR008116 SSDP_DNA-bd
     IPR006594 LisH_dimerisation

    Graphical View of Domain Structure for InterPro Entry Q9BWG4

    ProtoNet protein and cluster: Q9BWG4

    2 Blocks protein domains:
    IPB006594 Lissencephaly type-1-like homology motif
    IPB007591 Single-stranded DNA-binding protein


    UniProtKB/Swiss-Prot: SSBP4_HUMAN, Q9BWG4
    Similarity: Contains 1 LisH domain


    Find genes that share domains with SSBP4           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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         Gene Ontology (GO): 2 molecular function terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003697single-stranded DNA binding IEA--
    GO:0005515protein binding ----
         
    Find genes that share ontologies with SSBP4           About GenesLikeMe


    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for SSBP4
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       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for SSBP4
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for SSBP4

    miRNA
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    2 qRT-PCR Assays for microRNAs that regulate SSBP4:
    hsa-miR-146a* hsa-miR-3921
    SwitchGear 3'UTR luciferase reporter plasmidSSBP4 3' UTR sequence
    Inhib. RNA
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    OriGene RNAi products in human, mouse, rat for SSBP4
    Predesigned siRNA for gene silencing in human, mouse, rat SSBP4

    Gene Editing
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    GenScript: all cDNA clones in your preferred vector (see all 2): SSBP4 (NM_032627)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for SSBP4
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat SSBP4

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    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    SSBP4_HUMAN, Q9BWG4: Nucleus (By similarity)
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    cytosol2
    nucleus2

    Gene Ontology (GO): 1 cellular component term:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IEA--

    Find genes that share ontologies with SSBP4           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for SSBP4
    Interactions:

        Search GeneGlobe Interaction Network for SSBP4

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for SSBP4 (Q9BWG43 ENSP000002700614) via UniProtKB, MINT, STRING, and/or I2D (see all 27)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    IL36RNQ9UBH03, ENSP000002592124I2D: score=3 STRING: ENSP00000259212
    LDB1Q86U703, ENSP000003924664I2D: score=3 STRING: ENSP00000392466
    ISL1P613713, ENSP000002306584I2D: score=1 STRING: ENSP00000230658
    LDB2O436793, ENSP000003067724I2D: score=1 STRING: ENSP00000306772
    TRIM33Q9UPN93, ENSP000003512504I2D: score=2 STRING: ENSP00000351250
    About this table

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for SSBP4



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for SSBP4 gene (2 alternative transcripts): 
    NM_001009998.3  NM_032627.4  

    Unigene Cluster for SSBP4:

    Single stranded DNA binding protein 4
    Hs.515259  [show with all ESTs]
    Unigene Representative Sequence: NM_032627
    14 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000597724 ENST00000348495(uc002niz.3) ENST00000270061(uc010ebp.3 uc002niy.3)
    ENST00000601357 ENST00000598159 ENST00000607020 ENST00000601614 ENST00000602088
    ENST00000600628 ENST00000600244 ENST00000601919 ENST00000601444 ENST00000593641
    ENST00000599699
    miRNA
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    2 qRT-PCR Assays for microRNAs that regulate SSBP4:
    hsa-miR-146a* hsa-miR-3921
    SwitchGear 3'UTR luciferase reporter plasmidSSBP4 3' UTR sequence
    Inhib. RNA
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    Predesigned siRNA for gene silencing in human, mouse, rat SSBP4
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    GenScript: all cDNA clones in your preferred vector (see all 2): SSBP4 (NM_032627)
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      QuantiTect SYBR Green Assays in human, mouse, rat SSBP4
      QuantiFast Probe-based Assays in human, mouse, rat SSBP4

    Additional mRNA sequence: BC000274.1 

    22 DOTS entries:

    DT.97796310  DT.91758900  DT.437496  DT.97862520  DT.100038849  DT.121437695  DT.121437742  DT.97862521 
    DT.91758899  DT.100881971  DT.91891319  DT.100038854  DT.91758910  DT.91944448  DT.100759527  DT.121088786 
    DT.121437696  DT.121437741  DT.95256514  DT.97783028  DT.121437726  DT.40211222 

    Selected AceView cDNA sequences (see all 383):

    BM767098 BM743970 CA776447 BI712958 BM766693 BE676669 BM553378 AW009765 
    CA776678 BM760519 BM766688 BM767434 BM766698 AI923697 BM785461 BQ086210 
    AW005413 BM767136 BM794000 CA941973 AI972945 BM766714 BM559477 BM767117 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for SSBP4 (see all 14)    About this scheme

    ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9a · 9b ^ 10 ^ 11a · 11b ^ 12a · 12b ^ 13a · 13b ^ 14 ^ 15 ^ 16 ^ 17 ^ 18a · 18b ^ 19a · 19b · 19c ^
    SP1:                    -                       -           -                                                                                               -   
    SP2:                    -                       -           -                       -     -           -     -     -     -     -     -     -     -           -   
    SP3:                    -                       -           -     -                                                                                             
    SP4:                                                                                                                                                            
    SP5:        -     -     -                       -           -     -                                                                                             

    ExUns: 20a · 20b ^ 21 ^ 22a · 22b
    SP1:  -                           
    SP2:  -                           
    SP3:                              
    SP4:                              
    SP5:                              


    ECgene alternative splicing isoforms for SSBP4

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    SSBP4 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: AGCGTGTGAT
    SSBP4 Expression
    About this image

    SSBP4 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    SSBP4 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.515259
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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for SSBP4

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of animals.

    Orthologs for SSBP4 gene from Selected species (see all 12)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Ssbp41 , 5 single stranded DNA binding protein 41, 5 85.27(n)1
    89.23(a)1
      8 (34.15 cM)5
    769001  NM_133772.21  NP_598533.21 
     705974915 
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia LOC1004889611 single-stranded DNA-binding protein 3-like 72.33(n)
    81.62(a)
      100488961  XM_002940660.2  XP_002940706.2 
    zebrafish
    (Danio rerio)
    Actinopterygii ssbp41 single stranded DNA binding protein 4 73.09(n)
    81.28(a)
      321750  NM_001202512.1  NP_001189441.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta Ssdp6
    Sequence-specific single-stranded DNA-binding prot...
    43(a)
    1 → many
    3R(14018216-14025317)
    worm
    (Caenorhabditis elegans)
    Secernentea sam-106
    Protein SAM-10, isoform a
    22(a)
    1 → many
    II(13322013-13330825) WBGene00012990


    ENSEMBL Gene Tree for SSBP4 (if available)
    TreeFam Gene Tree for SSBP4 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for SSBP4 gene
    SSBP22  SSBP32  
    3 SIMAP similar genes for SSBP4 using alignment to 8 protein entries:     SSBP4_HUMAN (see all proteins):
    SSDP4    SSBP3    SSBP2

    Find genes that share paralogs with SSBP4           About GenesLikeMe


    5 Pseudogenes.org Pseudogenes for SSBP4
    PGOHUM00000247697 PGOHUM00000248030 PGOHUM00000259249 PGOHUM00000246369 PGOHUM00000240905


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for SSBP4 (see all 362)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 19 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs2008165451,2
    C--18528197(-) TCCCCA/CGGGCC 2 -- us2k10--------
    rs739231121,2
    C--18528201(+) CTGGGG/AAGCCA 2 -- us2k12Minor allele frequency- A:0.04WA 120
    rs755815751,2
    C--18528247(+) GGGGCA/CTCTGC 2 -- us2k13Minor allele frequency- C:0.14NA WA 240
    rs1396964951,2
    --18528276(+) TTGTCA/GCCAAG 2 -- us2k10--------
    rs795579201,2
    C--18528362(+) GAGCGG/ATCATT 2 -- us2k13Minor allele frequency- A:0.16NA WA 240
    rs1884327331,2
    --18528430(+) GGGCCC/GTCGCC 2 -- us2k10--------
    rs1926624311,2
    C--18528482(+) GCCGAC/GCCGGG 2 -- us2k10--------
    rs1829706611,2
    --18528518(+) CTGGGA/GGAAGG 2 -- us2k10--------
    rs1414132341,2
    C--18528858(+) CGGAG-/ACACACA 2 -- us2k10--------
    rs1497693821,2
    --18528917(+) CGGCCA/CGACTC 2 -- us2k10--------

    HapMap Linkage Disequilibrium report for SSBP4 (18529674 - 18545372 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 6 variations for SSBP4:    About this table    
    Variant IDTypeSubtypePubMed ID
    esv2677302CNV Deletion23128226
    nsv911276CNV Loss21882294
    nsv911275CNV Loss21882294
    dgv3783n71CNV Loss21882294
    dgv3782n71CNV Loss21882294
    nsv911274CNV Loss21882294

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing SSBP4
    DNA2.0 Custom Variant and Variant Library Synthesis for SSBP4

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 607391    OMIM disorders: --


    Find genes that share disorders with SSBP4           About GenesLikeMe

    Genetic Association Database (GAD): SSBP4
    Human Genome Epidemiology (HuGE) Navigator: SSBP4 (3 documents)

    Export disorders for SSBP4 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for SSBP4 gene, integrated from 10 sources (see all 21):
    (articles sorted by number of sources associating them with SSBP4)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. A novel, evolutionarily conserved gene family with putative sequence- specific single-stranded DNA-binding activity. (PubMed id 12079286)1, 2, 3 Castro P.D.... Nagarajan L. (Genomics 2002)
    2. Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study. (PubMed id 20628086)1, 4 Bailey S.D....Anand S. (Diabetes Care 2010)
    3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    4. Large-scale genotyping identifies 41 new loci associated with breast cancer risk. (PubMed id 23535729)1 Michailidou K....Easton D.F. (Nat. Genet. 2013)
    5. N-terminal acetylome analyses and functional insights of the N- terminal acetyltransferase NatB. (PubMed id 22814378)2 Van Damme P....Aldabe R. (Proc. Natl. Acad. Sci. U.S.A. 2012)
    6. Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. (PubMed id 20068231)2 Olsen J.V....Mann M. (Sci. Signal. 2010)
    7. TIF1gamma controls erythroid cell fate by regulating transcription elongation. (PubMed id 20603019)1 Bai X....Zon L.I. (Cell 2010)
    8. An atlas of combinatorial transcriptional regulation in mouse and man. (PubMed id 20211142)1 Ravasi T....Hayashizaki Y. (Cell 2010)
    9. Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. (PubMed id 19413330)2 Gauci S....Mohammed S. (Anal. Chem. 2009)
    10. Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. (PubMed id 19690332)2 Mayya V.... Han D.K. (Sci. Signal. 2009)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 170463 HGNC: 15676 AceView: SSBP4 Ensembl:ENSG00000130511 euGenes: HUgn170463
    ECgene: SSBP4 H-InvDB: SSBP4

    (According to HUGE)
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    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for SSBP4 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
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    IP news from LifeMap Sciences, Inc.)
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    Patent Information for SSBP4 gene:
    Search GeneIP for patents involving SSBP4

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
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