SPRED3 Gene
protein-coding GIFtS: 43
GCID: GC19P038880
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|
sprouty-related, EVH1 domain containing 3
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Aliases
for SPRED3 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| Sprouty-Related, EVH1 Domain Containing 31 2 | | Eve-32 | | Spred-33 | | Sprouty-Related, EVH1 Domain-Containing Protein 32 | | EVE-33 | | Spred-33 |
Export aliases for SPRED3 gene to outside databasesPrevious GC identifers: GC19U900378 GC19P043574 GC19P035328 |
Summaries
for SPRED3 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
Entrez Gene summary for SPRED3:
This gene encodes a protein with a C-terminal Sprouty-like cysteine-rich domain (SRY) and an N-terminalEna/Vasodilator-stimulated phosphoprotein (VASP) homology-1 (EVH-1) domain. The encoded protein is a member of afamily of proteins that negatively regulates mitogen-activated protein (MAP) kinase signaling, particularly duringorganogenesis. Alternative splicing results in multiple transcript variants. (provided by RefSeq, Jul 2012)
UniProtKB/Swiss-Prot: SPRE3_HUMAN, Q2MJR0Function: Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase (By similarity)
Gene Wiki entry for SPRED3
|
Genomic Views
for SPRED3 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000019.9 NC_018930.1 NT_011109.16
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the SPRED3 gene promoter:
E2F-4 E2F-3a Elk-1 E2F-5 Nkx2-5 E2F-2 NRF-2 E2F-1 E2F Zic3
Other transcription factors
Search SABiosciences Chromatin IP Primers for SPRED3
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat SPRED3 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 19q13 Ensembl cytogenetic band: 19q13.2 HGNC cytogenetic band: 19q13SPRED3 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 19 GeneLoc Exon Structure GeneLoc location for GC19P038880: view genomic region
(about GC identifiers)
Start:
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38,879,061 bp from pter |
End:
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38,886,881 bp from pter |
Size:
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7,821 bases |
Orientation:
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plus strand |
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Proteins
for SPRED3 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: SPRE3_HUMAN, Q2MJR0 (See
protein sequence)Recommended Name: Sprouty-related, EVH1 domain-containing protein 3 Size: 410 amino acids; 42670 Da
Subunit: Interacts with Ras (By similarity)
Subcellular location: Membrane; Peripheral membrane protein (By similarity)
Secondary accessions: Q2MJR1Alternative splicing: 2 isoforms: Q2MJR0-1 Q2MJR0-2 Explore the universe of human proteins at neXtProt for SPRED3: NX_Q2MJR0
Post-translational modifications:
Phosphorylated on tyrosine (By similarity)1
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q2MJR0
SPRED3 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_001035987.1
ENSEMBL proteins: ENSP00000467540 ENSP00000465934 ENSP00000466568 ENSP00000467532 ENSP00000345405 ENSP00000380086 ENSP00000465713
Human Recombinant Protein Products:
Gene Ontology (GO): 1 cellular component term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0005886 | plasma membrane |
IEA | -- |
SPRED3 for ontologies About GeneDecksing
SPRED3 Antibody Products:
Assay Products for SPRED3: |
Protein
Domains /
Families
for SPRED3 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
SPRED3 for domains About GeneDecksing
4 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry Q2MJR0ProtoNet protein and cluster: Q2MJR0 1 Blocks protein family: IPB007875 Sprouty
UniProtKB/Swiss-Prot: SPRE3_HUMAN, Q2MJR0Similarity: Contains 1 KBD domainSimilarity: Contains 1 SPR (sprouty) domainSimilarity: Contains 1 WH1 domain |
Function
for SPRED3 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: SPRE3_HUMAN, Q2MJR0Function: Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase (By similarity)
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for SPRED3 (see all 3)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for SPRED3 (see all 2)
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Synthesis Service for SPRED3 | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat SPRED3 |
|
Pathways & Interactions
for SPRED3 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for SPRED3
STRING Interaction
Network Preview (showing 1 interactants - click image to see more details)
1 Interacting protein for SPRED3 (Q2MJR03 ENSP000003454054) via UniProtKB, MINT, STRING, and/or I2DAbout this table
Gene Ontology (GO): 2 biological process terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0000188 | inactivation of MAPK activity |
IEA | -- | | GO:0007275 | multicellular organismal development |
IEA | -- |
SPRED3 for ontologies About GeneDecksing
|
Drugs & Compounds
for SPRED3 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for SPRED3
Search CenterWatch for drugs/clinical trials and news about SPRED3 / SPRE3
|
Transcripts
for SPRED3 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for SPRED3 gene (2 alternative transcripts): NM_001042522.2 NM_001039616.1 Unigene Cluster for SPRED3: Sprouty-related, EVH1 domain containing 3 Hs.196011 [show with all ESTs]Unigene Representative Sequence: NM_0010425228 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000587013 ENST00000587564 ENST00000586301 ENST00000587947 ENST00000338502(uc002oim.3)
ENST00000396877(uc002oil.1) ENST00000586958 ENST00000590962
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for SPRED3 (see all 3)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for SPRED3 (see all 2)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector (see all 2): SPRED3 (NM_001042522) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for SPRED3 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat SPRED3 |
Additional cDNA sequence: BC151136.1 DQ323927.1 DQ323928.1 NR_073032.1 7 DOTS entries: DT.75100748 DT.97785874 DT.121459297 DT.95150352 DT.95203186 DT.95148822 DT.100661048
GeneLoc Exon Structure
|
Expression
for SPRED3 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| SPRED3 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: GTGCGGGTCC
About this image
See SPRED3 Protein Expression from SPIRE MOPED and PaxDB
SOURCE GeneReport for Unigene cluster: Hs.196011
UniProtKB/Swiss-Prot: SPRE3_HUMAN, Q2MJR0Tissue specificity: Expression is restricted to liver
SABiosciences Custom PCR Arrays for SPRED3
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for SPRED3
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat SPRED3 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat SPRED3 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat SPRED3 |
Orthologs
for SPRED3 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of animals.
Orthologs for SPRED3 gene from 3/16 species (see all 16) About this table
| Organism |
Taxonomic
classification |
Gene |
Description |
Human
Similarity |
Orthology
Type |
Details |
lizard
(Anolis carolinensis) |
Reptilia |
SPRED36 |
-- |
40(a) |
1 ↔ 1 |
GL343427.1(438718-446593) |
fruit fly
(Drosophila melanogaster) |
Insecta |
Spred6 |
Sprouty-related protein with EVH-1 domain |
27(a) |
1 → many |
2R(10647151-10656927) |
worm
(Caenorhabditis elegans) |
Secernentea |
unc-346 |
UNCoordinated family member (unc-34) |
10(a) |
1 → many |
V(982535-995405) |
ENSEMBL Gene Tree for SPRED3 (if available)
TreeFam Gene Tree for SPRED3 (if available) |
Paralogs
for SPRED3 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for SPRED3 gene
- SPRED12 SPRED22
3 SIMAP similar genes for SPRED3 using alignment to 5 protein entries: SPRE3_HUMAN (see all proteins):SPRED2 EVL SPRED1
SPRED3 for paralogs About GeneDecksing
|
Genomic Variants
for SPRED3 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 19 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for SPRED3 (38879061 - 38886881 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for SPRED3: -- | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing SPRED3 |
|
Disorders
/ Diseases
for SPRED3 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
SPRED3 for disorders About GeneDecksing
OMIM gene information: 609293
OMIM disorders: --
2  diseases for SPRED3: About MalaCardsglioblastoma neuronitis
Human Genome Epidemiology (HuGE) Navigator: SPRED3 (1 document)
Export disorders for SPRED3 gene to outside databases
|
Publications
for SPRED3 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for SPRED3 gene integrated from 9 sources:
(articles sorted by number of sources associating them with SPRED3) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- Eve-3: a liver enriched suppressor of Ras/MAPK signaling. (PubMed id 16478641)1, 2 King J.A.J.... Hovens C.M. (2006)
- Mutation analysis of CBL-C and SPRED3 on 19q in human glioblastoma. (PubMed id 14618415)1, 3 Mizoguchi M....Louis D.N. (2004)
- FGF signaling inhibitor, SPRY4, is evolutionarily conserved target of WNT signaling pathway in progenitor cells. (PubMed id 16465403)1, 9 Katoh Y. and Katoh M. (2006)
- Sprouty proteins: modified modulators, matchmakers or missing links? (PubMed id 19423641)1 Guy G.R....Chow S.Y. (2009)
- Spred-2 steady-state levels are regulated by phosphorylation and Cbl-mediated ubiquitination. (PubMed id 17094949)1 Lock P....Stylli S.S. (2006)
- The DNA sequence and biology of human chromosome 19. (PubMed id 15057824)2 Grimwood J.... Lucas S.M. (2004)
- Molecular cloning of mammalian Spred-3 which suppresses tyrosine kinase-mediated Erk activation. (PubMed id 12646235)1 Kato R....Yoshimura A. (2003)
- Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)
|
External Searches for SPRED3 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing SPRED3 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing SPRED3 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing SPRED3 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for SPRED3 | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for SPRED3 gene:
Search GeneIP for patents involving SPRED3
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for SPRED3 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
| | |  | OriGene Antibodies for SPRED3 | | OriGene shRNA RFP for SPRED3 | | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for SPRED3 | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for SPRED3 | | | OriGene Protein Over-expression Lysate for SPRED3 | | Browse OriGene Fluorogenic Cell Assay Kits | | | OriGene siRNA for SPRED3 | | Browse 3'-UTR reporter clones for miRNA target validation | | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for SPRED3 | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for SPRED3 | | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | | OriGene Purified Protein for SPRED3 | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling | | | OriGene Custom Antibody Services for SPRED3 | | OriGene Custom Protein Services for SPRED3 | | | OriGene Custom Immunoassay Development | | |
| |
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| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat SPRED3 | | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing SPRED3 | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat SPRED3 | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat SPRED3 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat SPRED3 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat SPRED3 |
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| | | | Search Tocris compounds for SPRED3 |
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 | | | SPRED3 Proteins, Antibodies, CLIAs, and ELISAs |
| | | |  |  |  | | | | ThermoFisher Antibodies for SPRED3 |
| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat SPRED3 |
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