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Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

SPRED2 Gene

protein-coding GIFtS: 60
GCID: GC02M065537

sprouty-related, EVH1 domain containing 2

Explore 9 diseases affiliated with
SPRED2 via

MalaCards

our new
Human Malady Compendium

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
Sprouty-Related, EVH1 Domain Containing 2¹ ²
Spred-2¹ ² ³
FLJ21897¹
FLJ31917¹
Sprouty Protein With EVH-1 Domain 2, Related Sequence²
Sprouty-Related, EVH1 Domain-Containing Protein 2²

External Ids:	HGNC: 17722¹	Entrez Gene: 200734²	Ensembl: ENSG00000198369⁷	OMIM: 609292⁵	UniProtKB: Q7Z698³

Export aliases for SPRED2 gene to outside databases

Previous GC identifers: GC02M065495 GC02M065512 GC02M065449 GC02M065272

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for SPRED2:

SPRED2 is a member of the Sprouty (see SPRY1; MIM 602465)/SPRED family of proteins that regulate growth factor-induced

activation of the MAP kinase cascade (see MAPK1; MIM 176948) (Nonami et al., 2004 (PubMed 15465815)).(supplied by

OMIM, Mar 2008)

UniProtKB/Swiss-Prot: SPRE2_HUMAN, Q7Z698

Function: Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase

Gene Wiki entry for SPRED2

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000002.11 NC_018913.1 NT_022184.15

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the SPRED2 gene promoter:
Bach1 FOXF2 Pax-5 NF-AT FOXL1 NF-AT4 SRY NF-AT2 NF-AT3 NF-AT1
Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: SPRED2 promoter sequence

Search SABiosciences Chromatin IP Primers for SPRED2

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat SPRED2

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 2p14 Ensembl cytogenetic band: 2p14 HGNC cytogenetic band: 2p14

SPRED2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
SPRED2 gene location

GeneLoc information about chromosome 2 GeneLoc Exon Structure

GeneLoc location for GC02M065537: view genomic region (about GC identifiers)

Start:	65,537,985 bp from pter	End:	65,659,771 bp from pter
Size:	121,787 bases	Orientation:	minus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: SPRE2_HUMAN, Q7Z698 (See protein sequence)

Recommended Name: Sprouty-related, EVH1 domain-containing protein 2

Size: 418 amino acids; 47558 Da

Subunit: Interacts with Ras (By similarity). Homodimer and heterodimer. Able to interact with SPRED1 to form

heterodimers

Subcellular location: Membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Cytoplasmic vesicle,

secretory vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential). Cytoplasm. Note=Especially

found in the cytoplasm of the stratum spinosum cells called membrane-coated granules

1 PDB 3D structure from and Proteopedia for SPRED2:

2JP2 (3D)

Secondary accessions: A1L3V4 B7Z5K7 D6W5F7 E9PEP0 Q2NKX6

Alternative splicing: 2 isoforms: Q7Z698-1 Q7Z698-2 (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for SPRED2: NX_Q7Z698

Post-translational modifications:

Phosphorylated on tyrosine. Phosphorylation of Tyr-228 and Tyr-231 are required for ubiquitination¹

Ubiquitinated; leading to degradation by the proteasome¹

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_Q7Z698

SPRED2 Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins (2 alternative transcripts):

NP_001121682.1 NP_861449.2

ENSEMBL proteins:

ENSP00000348753 ENSP00000393697 ENSP00000390595 ENSP00000407627 ENSP00000414551

ENSP00000406481 ENSP00000393148

Human Recombinant Protein Products for SPRED2:

	Browse Purified and Recombinant Proteins at EMD Millipore
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	GenScript Custom Purified and Recombinant Proteins Services for SPRED2
	Novus Biologicals SPRED2 Proteins Novus Biologicals SPRED2 Lysate
	Browse Sino Biological Recombinant Proteins
	Browse ProSpec Recombinant Proteins
	Uscn Proteins for SPRED2

Gene Ontology (GO): 4 cellular component terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005886	plasma membrane	IEA	--
GO:0016020	membrane	--	--
GO:0030658	transport vesicle membrane	IEA	--
GO:0031410	cytoplasmic vesicle	--	--

SPRED2 for ontologies About GeneDecksing

SPRED2 Antibody Products:

	EMD Millipore Mono- and Polyclonal Antibodies for the study of SPRED2
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	ThermoFisher Antibodies for SPRED2

Assay Products for SPRED2:

	Browse Kits and Assays available from EMD Millipore
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	Browse R&D Systems for biochemical assays
	GenScript Custom Assay Services for SPRED2
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for SPRED2

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

SPRED2 for domains About GeneDecksing

4 InterPro domains/families:

IPR007875 Sprouty

IPR011993 PH_like_dom

IPR000697 EVH1

IPR023337 KBD

Graphical View of Domain Structure for InterPro Entry Q7Z698

ProtoNet protein and cluster: Q7Z698

1 Blocks protein family: IPB007875 Sprouty

UniProtKB/Swiss-Prot: SPRE2_HUMAN, Q7Z698

Similarity: Contains 1 KBD domain

Similarity: Contains 1 SPR (sprouty) domain

Similarity: Contains 1 WH1 domain

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, Sirion Biotech, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, Sirion Biotech, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Molecular Function:

UniProtKB/Swiss-Prot Summary: SPRE2_HUMAN, Q7Z698

Function: Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase

Gene Ontology (GO): 4 molecular function terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005173	stem cell factor receptor binding	ISS	--
GO:0005515	protein binding	IPI	19822672
GO:0019901	protein kinase binding	IPI	--
GO:0030291	protein serine/threonine kinase inhibitor activity	ISS	--

SPRED2 for ontologies About GeneDecksing

Phenotypes:
2 GenomeRNAi human phenotypes for SPRED2:

Decreased focal adhesion (FA)

Decreased viability with pacli

5 MGI mutant phenotypes (inferred from 2 alleles) (MGI details for Spred2):

growth/size

hematopoietic system

limbs/digits/tail

reproductive system

skeleton

SPRED2 for phenotypes About GeneDecksing

Animal Models:
Mouse knock-out Spred2^tm1Taga for SPRED2

inGenious Targeting Laboratory - Customizable classic, inducible, and humanized mouse model solutions for SPRED2

miRNA Products:		OriGene 3'-UTR Clone (see all 2): SPRED2 Browse MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat SPRED2
		8/32 QIAGEN miScript miRNA Assays for microRNAs that regulate SPRED2 (see all 32):
		hsa-miR-485-5p hsa-miR-3146 hsa-miR-509-5p hsa-miR-487a hsa-miR-485-3p hsa-miR-938 hsa-miR-340 hsa-miR-218

		SwitchGear 3'UTR luciferase reporter plasmid: SPRED2 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for SPRED2 (see all 7) OriGene shRNA RFP: SPRED2 OriGene siRNA: SPRED2
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat SPRED2
		Sirion Biotech Custom design and validation of potent shRNA sequences against SPRED2

Gene Editing Products:			DNA2.0 Custom Protein Engineering Service for SPRED2
			Sirion Biotech Customized adenovirus for overexpression of SPRED2 Sirion Biotech Customized adenovirus for potent knockdown of SPRED2

Clone Products:		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for SPRED2 (see all 4) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for SPRED2 (see all 2) OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector (see all 2): SPRED2 (NM_181784)
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		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat SPRED2

Cell Line Products:		GenScript Custom overexpressing Cell Line Services for SPRED2
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		Sirion Biotech Customized stable knockdown cell line services for SPRED2 Sirion Biotech Customized inducible knockdown cell line services for SPRED2 Sirion Biotech Customized stable overexpressing cell line services for SPRED2 Sirion Biotech Customized inducible overexpressing cell line services for SPRED2

In Situ Assay
Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for SPRED2

(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
About This Section

Unified GeneCards pathways About this table

See pathways by source

Super-pathway

contained gene-specific pathways

Signaling events mediated by Stem cell factor receptor (c-Kit)

Signaling events mediated by Stem cell factor receptor (c-Kit)

1.00

Kit Receptor Signaling Pathway

0.37

Jak-STAT signaling pathway

Jak-STAT signaling pathway

1.00

Pathway sources
See GeneCards unified pathways
Show all pathways

2 BioSystems Pathways for SPRED2

	Kit Receptor Signaling Pathway
	Signaling events mediated by Stem cell factor receptor (c-Kit)

1 Kegg Pathway (Kegg details for SPRED2):

Jak-STAT signaling pathway

SPRED2 for pathways About GeneDecksing

Interactions:

Search SABiosciences Gene Network Central^TM Interacting Genes and Proteins Networks for SPRED2

STRING Interaction Network Preview (showing 5 interactants - click image to see 7)

5/9 Interacting proteins for SPRED2 (Q7Z698^{2, 3} ENSP00000348753⁴) via UniProtKB, MINT, STRING, and/or I2D (see all 9)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
CBL	P22681²^,³, ENSP00000264033⁴	MINT-8031653 MINT-8034108 I2D: score=1 STRING: ENSP00000264033
KIT	P10721³, ENSP00000288135⁴	I2D: score=2 STRING: ENSP00000288135
RHOA	P61586³, ENSP00000400175⁴	I2D: score=2 STRING: ENSP00000400175
TESK1	Q15569³, ENSP00000338127⁴	I2D: score=1 STRING: ENSP00000338127
UBC	P0CG48³, ENSP00000344818⁴	I2D: score=1 STRING: ENSP00000344818

About this table

Gene Ontology (GO): 5/6 biological process terms (GO ID links to tree view) (see all 6): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0000188	inactivation of MAPK activity	ISS	--
GO:0007275	multicellular organismal development	IEA	--
GO:0009966	regulation of signal transduction	--	--
GO:0010801	negative regulation of peptidyl-threonine phosphorylation	IMP	--
GO:0043517	positive regulation of DNA damage response, signal transduction by p53 class mediator	ISS	--

SPRED2 for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section

Browse Small Molecules at EMD Millipore

Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for SPRED2
Search CenterWatch for drugs/clinical trials and news about SPRED2 / SPRE2

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from EMD Millipore,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, Sirion Biotech, microRNA from QIAGEN,
Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
About This Section

REFSEQ mRNAs for SPRED2 gene (2 alternative transcripts):

NM_001128210.1 NM_181784.2

Unigene Cluster for SPRED2:

Sprouty-related, EVH1 domain containing 2

Hs.59332 [show with all ESTs]

Unigene Representative Sequence: NM_181784

8 Ensembl transcripts including schematic representations, and UCSC links where relevant:

ENST00000356388(uc010fcw.3 uc002sdr.4 uc010fcx.1) ENST00000443619

ENST00000452315 ENST00000421087 ENST00000474228 ENST00000426832 ENST00000440972

ENST00000427238

miRNA Products:		OriGene 3'-UTR Clone (see all 2): SPRED2 Browse OriGene MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat SPRED2
		8/32 QIAGEN miScript miRNA Assays for microRNAs that regulate SPRED2 (see all 32):
		hsa-miR-485-5p hsa-miR-3146 hsa-miR-509-5p hsa-miR-487a hsa-miR-485-3p hsa-miR-938 hsa-miR-340 hsa-miR-218

		SwitchGear 3'UTR luciferase reporter plasmid: SPRED2 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for SPRED2 (see all 7) OriGene shRNA RFP: SPRED2 OriGene siRNA: SPRED2
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat SPRED2
		Sirion Biotech Custom design and validation of potent shRNA sequences against SPRED2

Clone Products:		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for SPRED2 (see all 4) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for SPRED2 (see all 2) OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector (see all 2): SPRED2 (NM_181784)
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for SPRED2
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat SPRED2

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for SPRED2 Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat SPRED2
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat SPRED2
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat SPRED2

Additional cDNA sequence:

AF052178.1 AK025550.1 AK056479.1 AK299079.1 AK307714.1 AY299090.1 BC029300.1 BC111495.1

BC130292.1 BC136334.1

11 DOTS entries:

DT.451903 DT.40106961 DT.201521 DT.95098343 DT.101958206 DT.95098342 DT.120962114 DT.120962124

DT.92425600 DT.95098292 DT.95190414

24/246 AceView cDNA sequences (see all 246):

AA736862 BX505919 CA448426 BM757933 BQ447380 BM970804 AA635014 BC029300

BM470807 AI075225 AA478058 AA766026 BM547516 AA907128 BE856977 CF528598

AI015646 BQ268179 CK905996 AF052178 BQ268276 AA864731 AA743455 AI017816

GeneLoc Exon Structure

4 Alternative Splicing Database (ASD) splice patterns (SP) for SPRED2 About this scheme

ExUns:	1	^	2	^	3	^	4	^	5	^	6a	·	6b	·	6c
SP1:					-
SP2:
SP3:			-		-
SP4:					-				-

ECgene alternative splicing isoforms for SPRED2

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

SPRED2 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: GGGACATTTA

About this image

SPRED2 expression in embryonic tissues and stem cells
Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine About this table

Stem Cell Differentiation: 1 LifeMap Cell

Name

Category

Definitive endoderm-like cells (A scalable, suspensi...)

Expression:

Positive

Negative

Selective marker

Experimental details:

Curated

Microarrays

In-situ hybridization

See SPRED2 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for SPRED2

SOURCE GeneReport for Unigene cluster: Hs.59332

UniProtKB/Swiss-Prot: SPRE2_HUMAN, Q7Z698

Tissue specificity: Expressed in liver, skin, small intestine, salivary gland and prostate

SABiosciences Custom PCR Arrays for SPRED2

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		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat SPRED2

In Situ
Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for SPRED2

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the common ancestor of animals.

Orthologs for SPRED2 gene from 6/22 species (see all 22) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
chicken (Gallus gallus)	Aves	SPRED2¹	sprouty-related, EVH1 domain containing 2	76.71(n) 86.42(a)		421271 XM_419341.3 XP_419341.3
lizard (Anolis carolinensis)	Reptilia	SPRED2⁶	--	80(a)	1 ↔ 1	1(124575334-124603876)
African clawed frog (Xenopus laevis)	Amphibia	BM261751.1²	--	81.24(n)		BM261751.1
zebrafish (Danio rerio)	Actinopterygii	wufb93f08²	Transcribed sequence with weak similarity to protein more	77.06(n)		57044097
fruit fly (Drosophila melanogaster)	Insecta	Spred⁶	Sprouty-related protein with EVH-1 domain	31(a)	1 → many	2R(10647151-10656927)
worm (Caenorhabditis elegans)	Secernentea	unc-34⁶	UNCoordinated family member (unc-34)	11(a)	1 → many	V(982535-995405)

ENSEMBL Gene Tree for SPRED2 (if available)
TreeFam Gene Tree for SPRED2 (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
About This Section

Paralogs for SPRED2 gene

SPRED3² SPRED1²

3 SIMAP similar genes for SPRED2 using alignment to 6 protein entries: SPRE2_HUMAN (see all proteins):

SPRED1 EVL SPRED3

SPRED2 for paralogs About GeneDecksing

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section

SNP ID	Valid	Clinical significance	Chr 2 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs140095091^1,2		--	65537789(+)	`CATCCA/GAGCCC`	2	--	int¹		0	--	--	--	--
rs145416685^1,2		--	65537881(+)	`TCCTTC/TGGCAC`	2	--	int¹		0	--	--	--	--
rs184918658^1,2		--	65537882(+)	`CCTTCA/GGCACA`	2	--	int¹		0	--	--	--	--
rs147701515^1,2		--	65537934(+)	`GCCAAC/TGCTCT`	2	--	int¹		0	--	--	--	--
rs187840999^1,2		--	65537935(+)	`CCAACA/GCTCTA`	2	--	int¹		0	--	--	--	--
rs58799744^1,2	F	--	65537937(+)	`AACGCT/GCTAGC`	2	--	int¹		1		WA	118
rs140929168^1,2		--	65537981(+)	`AGCCAG/TCGATC`	2	--	int¹		0	--	--	--	--
rs149783184^1,2		--	65538070(+)	`AAAAGC/TTAAGA`	2	--	ut3¹		0	--	--	--	--
rs114625088^1,2	F	--	65538235(+)	`GGGGGG/ATCGGG`	2	--	ut3¹		1		NA	120
rs145090112^1,2		--	65538237(+)	`GGGGTC/TGGGGA`	2	--	ut3¹		0	--	--	--	--

HapMap Linkage Disequilibrium report for SPRED2 (65537985 - 65659771 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 1 variation for SPRED2
1 CNV: 5985

SABiosciences Cancer Mutation PCR Assays

QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing SPRED2

DNA2.0 Custom Variant and Variant Library Synthesis for SPRED2

(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

SPRED2 for disorders           About GeneDecksing

OMIM gene information: 609292    OMIM disorders: --

9 diseases for SPRED2:    About MalaCards

noonan syndrome dwarfism rheumatoid arthritis hematopoiesis

hepatocellular carcinoma arthritis carcinoma prostatitis

neuronitis

Human Genome Epidemiology (HuGE) Navigator: SPRED2 (7 documents)

Export disorders for SPRED2 gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
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PubMed articles for SPRED2 gene, integrated from 9 sources (see all 33):
(articles sorted by number of sources associating them with SPRED2)

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Spred-2 steady-state levels are regulated by phosphorylation and Cbl-mediated ubiquitination. (PubMed id 17094949)^{1, 2, 9} Lock P....Stylli S.S. (2006)
Distinct requirements for the Sprouty domain for functional activity of Spred proteins. (PubMed id 15683364)^{1, 2} King J.A.J.... Hovens C.M. (2005)
Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)^{1, 2} Ota T.... Sugano S. (2004)
1H, 13C and 15N resonance assignment of the human Spred2 EVH1 domain. (PubMed id 15213456)^{1, 2} Zimmermann J.... Ball L.J. (2004)
Expression and subcellular localization of Spred proteins in mouse and human tissues. (PubMed id 15580519)^{1, 2} Engelhardt C.M.... Schuh K. (2004)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{1, 2} Gerhard D.S....Malek J. (2004)
Methods for quantification of in vivo changes in prote in ubiquitination following proteasome and deubiquitinase inhibition. (PubMed id 22505724)¹ Udeshi N.D....Carr S.A. (2012)
Tyrosines 303/343/353 within the Sprouty-related domai n of Spred2 are essential for its interaction with p85 and inhibitory effect on Ras/ERK activation. (PubMed id 22305891)¹ Meng S....Wang C. (2012)
Placenta proteome analysis from Down syndrome pregnanc ies for biomarker discovery. (PubMed id 22777171)¹ Chen C.P....Chan H.L. (2012)
Sprouty2 and Spred1-2 proteins inhibit the activation of the ERK pathway elicited by cyclopentenone prostanoids. (PubMed id 21364986)¹ GarcA-a-DomA-nguez C.A....Rojas J.M. (2011)

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(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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Entrez Gene: 200734	HGNC: 17722	AceView: SPRED2	Ensembl:ENSG00000198369	euGenes: HUgn200734
ECgene: SPRED2	Kegg: 200734	H-InvDB: SPRED2

(According to HUGE)
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(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
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Name	Description
PharmGKB entry for SPRED2	Pharmacogenomics, SNPs, Pathways

Intellectual Property for SPRED2 gene
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Patent Information for SPRED2 gene:
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SPRED2 Gene

sprouty-related, EVH1 domain containing 2

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SPRED2 Gene

sprouty-related, EVH1 domain containing 2

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