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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

SPP1 Gene

protein-coding   GIFtS: 71
GCID: GC04P088896

Secreted Phosphoprotein 1

(Previous names: osteopontin, bone sialoprotein I)
(Previous symbols: BNSP, OPN)
Alzheimer's & Parkinson's Diseases Congress
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(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

Aliases
Secreted Phosphoprotein 11 2 3     nephropontin2
OPN1 2 3 5     Osteopontin/Immunoglobulin Alpha 1 Heavy Chain Constant Region Fusion
Protein2
BNSP1 2 3     Secreted Phosphoprotein 1 (Osteopontin, Bone Sialoprotein I, Early
T-Lymphocyte Activation 1)2
osteopontin1 2     SPP1/CALPHA1 Fusion2
Early T-Lymphocyte Activation 11 2     uropontin2
Urinary Stone Protein2 3     Nephropontin3
Bone Sialoprotein I1     SPP-13
BSPI2     Uropontin3
ETA-12     Bone Sialoprotein 13

External Ids:    HGNC: 112551   Entrez Gene: 66962   Ensembl: ENSG000001187857   OMIM: 1664905   UniProtKB: P104513   

Export aliases for SPP1 gene to outside databases

Previous GC identifers: GC04P089054 GC04P089198 GC04P089355 GC04P089253 GC04P089115 GC04P084643


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for SPP1 Gene:
The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The
encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is
found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine
that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding
different isoforms have been found for this gene. (provided by RefSeq, Oct 2011)

GeneCards Summary for SPP1 Gene: 
SPP1 (secreted phosphoprotein 1) is a protein-coding gene. Diseases associated with SPP1 include osteofibrous dysplasia, and papillary cystadenocarcinoma, and among its related super-pathways are Syndecan interactions and Toll-like receptor signaling pathway. GO annotations related to this gene include extracellular matrix binding and cytokine activity.

UniProtKB/Swiss-Prot: OSTP_HUMAN, P10451
Function: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably
important to cell-matrix interaction
Function: Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing
production of interleukin-10 and is essential in the pathway that leads to type I immunity (By similarity)

Gene Wiki entry for SPP1 (Osteopontin) Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 73), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000004.11  NC_018915.2  NT_016354.19  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the SPP1 gene promoter:
         AML1a   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 3): SPP1 promoter sequence
   Search SABiosciences Chromatin IP Primers for SPP1

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat SPP1


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 4q22.1   Ensembl cytogenetic band:  4q22.1   HGNC cytogenetic band: 4q22.1

SPP1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
SPP1 gene location

GeneLoc information about chromosome 4         GeneLoc Exon Structure

GeneLoc location for GC04P088896:  view genomic region     (about GC identifiers)

Start:
88,896,802 bp from pter      End:
88,904,563 bp from pter
Size:
7,762 bases      Orientation:
plus strand

(According to UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MAXQB RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Ontologies according to Gene Ontology Consortium 01 Oct 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
About This Section

UniProtKB/Swiss-Prot: OSTP_HUMAN, P10451 (See protein sequence)
Recommended Name: Osteopontin precursor  
Size: 314 amino acids; 35423 Da
Subunit: Ligand for integrin alpha-V/beta-3
Subcellular location: Secreted
2 PDB 3D structures from and Proteopedia for SPP1:
3CXD (3D)        3DSF (3D)    
Secondary accessions: B2RDA1 Q15681 Q15682 Q15683 Q4W597 Q567T5 Q8NBK2 Q96IZ1
Alternative splicing: 5 isoforms:  P10451-1   P10451-2   P10451-3   P10451-4   P10451-5   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for SPP1: NX_P10451

Explore proteomics data for SPP1 at MOPED 

Post-translational modifications:

  • UniProtKB: Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif
  • UniProtKB: N- and O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62
  • View modification sites using PhosphoSitePlus
  • View neXtProt modification sites for NX_P10451

  • SPP1 Protein expression data from MOPED1, PaxDb2 and MAXQB3 :    About this image 

    SPP1 Protein Expression
    REFSEQ proteins (5 alternative transcripts): 
    NP_000573.1  NP_001035147.1  NP_001035149.1  NP_001238758.1  NP_001238759.1  

    ENSEMBL proteins: 
     ENSP00000237623   ENSP00000378517   ENSP00000422973   ENSP00000354042  
    Reactome Protein details: P10451
    Human Recombinant Protein Products for SPP1: 
    Browse Purified and Recombinant Proteins at EMD Millipore
    R&D Systems Recombinant & Natural Proteins for SPP1 (Osteopontin/OPN)
    Browse recombinant and purified proteins available from Enzo Life Sciences
    OriGene Purified Protein for SPP1
    OriGene Protein Over-expression Lysate for SPP1
    OriGene MassSpec for SPP1 
    OriGene Custom Protein Services for SPP1
    GenScript Custom Purified and Recombinant Proteins Services for SPP1
    Novus Biologicals SPP1 Proteins
    Novus Biologicals SPP1 Lysates
    Sino Biological Recombinant Protein for SPP1
    Sino Biological Cell Lysate for SPP1 
    ProSpec Recombinant Protein for SPP1
    Browse Proteins at Cloud-Clone Corp. 

    Gene Ontology (GO): 5/7 cellular component terms (GO ID links to tree view) (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005576extracellular region TAS--
    GO:0005615extracellular space IDA15516325
    GO:0005737cytoplasm ----
    GO:0031988membrane-bounded vesicle IEA--
    GO:0042995cell projection IEA--

    SPP1 for ontologies           About GeneDecksing



    SPP1 Antibody Products: 
    EMD Millipore Mono- and Polyclonal Antibodies for the study of SPP1
    R&D Systems Antibodies for SPP1 (Osteopontin/OPN)
    OriGene Antibodies for SPP1
    OriGene Custom Antibody Services for SPP1
    GenScript Custom Superior Antibodies Services for SPP1
    Novus Biologicals SPP1 Antibodies
    Abcam antibodies for SPP1
    Browse Antibodies at Cloud-Clone Corp. 
    ThermoFisher Antibody for SPP1
    LSBio Antibodies in human, mouse, rat for SPP1 

    Assay Products for SPP1: 
    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for SPP1
    R&D Systems ELISAs for SPP1 (Osteopontin/OPN)         (see all)
    GenScript Custom Assay Services for SPP1
    Enzo Life Sciences assays for SPP1
    Browse ELISAs at Cloud-Clone Corp. 
    Browse CLIAs at Cloud-Clone Corp.


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section
    2 InterPro protein domains:
     IPR002038 Osteopontin
     IPR019841 Osteopontin_CS

    Graphical View of Domain Structure for InterPro Entry P10451

    ProtoNet protein and cluster: P10451

    1 Blocks protein domain: IPB002038 Osteopontin

    UniProtKB/Swiss-Prot: OSTP_HUMAN, P10451
    Similarity: Belongs to the osteopontin family


    SPP1 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, Vector BioLabs, and Sirion Biotech, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene.)
    About This Section

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: OSTP_HUMAN, P10451
    Function: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably
    important to cell-matrix interaction
    Function: Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing
    production of interleukin-10 and is essential in the pathway that leads to type I immunity (By similarity)

         Genatlas biochemistry entry for SPP1:
    secreted phosphoprotein 1 (osteopontin),regulated by CBFA1 and ETS1 in the skeletal tissue

         Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005125cytokine activity IEA--
    GO:0050840extracellular matrix binding IEA--
         
    SPP1 for ontologies           About GeneDecksing


    Phenotypes:
         2 GenomeRNAi human phenotypes for SPP1:
     Decreased CXCL12 induced migra  Decreased viability with pacli 

         15/17 MGI mutant phenotypes (inferred from 4 alleles(MGI details for Spp1) (see all 17):
     behavior/neurological  cardiovascular system  cellular  growth/size  hematopoietic system 
     homeostasis/metabolism  immune system  integument  liver/biliary system  mortality/aging 
     muscle  nervous system  renal/urinary system  reproductive system  respiratory system 

    SPP1 for phenotypes           About GeneDecksing

    Animal Models:
         MGI mouse knock-outs for SPP1: Spp1tm1Blh Spp1tm1Rit

       inGenious Targeting Laboratory - Custom generated mouse model solutions for SPP1 
       inGenious Targeting Laboratory - Custom generated inducible mouse model solutions for SPP1

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for SPP1 
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for SPP1 

    miRNA
    Products:
        
    miRTarBase miRNAs that target SPP1:
    hsa-mir-299-5p (MIRT004565)

    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat SPP1
    8 QIAGEN miScript miRNA Assays for microRNAs that regulate SPP1:
    hsa-miR-181c hsa-miR-3065-5p hsa-miR-33b hsa-miR-181a hsa-miR-181d hsa-miR-33a hsa-miR-181b hsa-miR-4262
    SwitchGear 3'UTR luciferase reporter plasmidSPP1 3' UTR sequence
    Inhib. RNA
    Products:
        
    Browse for Gene Knock-down Tools from EMD Millipore
    OriGene RNAi products in human, mouse, rat for SPP1
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat SPP1

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for SPP1
    Sirion Biotech Customized adenovirus for overexpression of SPP1

    Clone
    Products:
         
    Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
    OriGene clones in human, mouse for SPP1 (see all 21)
    OriGene ORF clones in mouse, rat for SPP1
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 3): SPP1 (NM_001040058)
    Sino Biological Human cDNA Clone for SPP1
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for SPP1
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat SPP1
    Sirion Biotech Customized lentivirus for stable overexpression of SPP1 
                         Customized lentivirus expression plasmids for stable overexpression of SPP1 

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for SPP1
    LifeMap BioReagents: cell lines associated with SPP1: Osteogenic Package SK11,
    Osteogenic Package SM30, PureStem 7SMOO32, NCr-fac & Meso Progenitor,
    PureStem SK11, NCr-fac & Meso-prx Progenitor
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for SPP1


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene).
    About This Section

    SuperPaths for SPP1 About   (see all 23)                                                                                              See pathways by source

    SuperPathContained pathways About
    1Non-integrin membrane-ECM interactions
    Non-integrin membrane-ECM interactions0.44
    Integrin cell surface interactions0.32
    ECM-receptor interaction0.42
    2Toll-like receptor signaling pathway
    Toll-like receptor signaling pathway0.93
    Toll-like receptor signaling pathway0.93
    3Focal Adhesion
    Focal Adhesion0.64
    Focal adhesion0.64
    4Integrin Pathway
    Integrin Pathway0.67
    UPA-UPAR Pathway0.56
    FAK1 Signaling0.67
    Inhibition of Angiogenesis by TSP10.50
    GnRH Signaling0.56
    Transendothelial Migration of Leukocytes0.38
    5Rho Family GTPases
    Rho Family GTPases0.61
    Molecular Mechanisms of Cancer0.51
    ERK Signaling0.61
    ILK Signaling0.49
    MAPK Signaling0.58

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    2 EMD Millipore Pathways for SPP1
        Development Hedgehog and PTH signaling pathways in bone and cartilage development
    Transcription Role of VDR in regulation of genes involved in osteoporosis

    5/15 Downloadable PowerPoint Slides of QIAGEN Pathway Central Maps for SPP1 (see all 15)
        MAPK Signaling
    Molecular Mechanisms of Cancer
    PTEN Pathway
    PI3K Signaling in B-Lymphocyte
    Transendothelial Migration of Leukocytes

    2 GeneGo (Thomson Reuters) Pathways for SPP1
        Development Hedgehog and PTH signaling pathways in bone and cartilage development
    Transcription Role of VDR in regulation of genes involved in osteoporosis

    5/11 BioSystems Pathways for SPP1 (see all 11)
        Focal Adhesion
    Osteopontin Signaling
    TGF Beta Signaling Pathway
    BDNF signaling pathway
    Endochondral Ossification


    5        Reactome Pathways for SPP1
        Extracellular matrix organization
    Integrin cell surface interactions
    Signal Transduction
    Signaling by PDGF
    Non-integrin membrane-ECM interactions


    4         Kegg Pathways  (Kegg details for SPP1):
        PI3K-Akt signaling pathway
    Focal adhesion
    ECM-receptor interaction
    Toll-like receptor signaling pathway


    SPP1 for pathways           About GeneDecksing

    Interactions:

        SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for SPP1

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    5/47 Interacting proteins for SPP1 (P104512, 3 ENSP000003785174) via UniProtKB, MINT, STRING, and/or I2D (see all 47)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    ABCF3Q9NUQ82, 3MINT-65157 I2D: score=4 
    UBQLN4Q9NRR52, 3, ENSP000003572924MINT-2863322 MINT-2863341 I2D: score=4 STRING: ENSP00000357292
    SGTAO437652, 3, ENSP000002215664MINT-66220 I2D: score=5 STRING: ENSP00000221566
    CTNNBL1Q8WYA62, 3, ENSP000003550504MINT-67203 I2D: score=5 STRING: ENSP00000355050
    PDLIM7Q9NR122, 3, ENSP000003480994MINT-66700 I2D: score=5 STRING: ENSP00000348099
    About this table

    Gene Ontology (GO): 5/16 biological process terms (GO ID links to tree view) (see all 16):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001503ossification ----
    GO:0001649osteoblast differentiation IEA--
    GO:0006954inflammatory response IEA--
    GO:0007155cell adhesion IEA--
    GO:0007566embryo implantation TAS16720713

    SPP1 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section

    SPP1 for compounds           About GeneDecksing

    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for SPP1 (OSTP)

    10/110 Novoseek inferred chemical compound relationships for SPP1 gene (see all 110)    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    calcium oxalate 78.9 137 19581305 (5), 10461108 (5), 12706004 (4), 16104927 (4) (see all 44)
    grgds 76.3 42 7522656 (6), 8972713 (4), 1421573 (2), 11787071 (1) (see all 17)
    alizarin 75.8 6 15165454 (1), 15271969 (1), 20413153 (1), 15956138 (1) (see all 6)
    hydroxyapatite 74.7 143 16007483 (9), 8010953 (4), 7615968 (4), 16011845 (4) (see all 58)
    1,25 dihydroxy vitamin d3 60.5 12 10404392 (1), 11834737 (1), 7571189 (1), 8900396 (1) (see all 12)
    lm 609 60.4 2 10726209 (1), 7522656 (1)
    nimorazole 59.5 2 16198981 (2)
    calcium 52.6 169 16145474 (4), 10460892 (4), 7988430 (4), 20144595 (3) (see all 91)
    titanium 52 14 20155835 (2), 18983324 (2), 17437306 (2), 11173803 (1) (see all 6)
    vitamin d 50 43 19246678 (2), 7708050 (1), 9556070 (1), 1665202 (1) (see all 28)

    Search CenterWatch for drugs/clinical trials and news about SPP1 / OSTP

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Sirion Biotech, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for SPP1 gene (5 alternative transcripts): 
    NM_000582.2  NM_001040058.1  NM_001040060.1  NM_001251829.1  NM_001251830.1  

    Unigene Cluster for SPP1:

    Secreted phosphoprotein 1
    Hs.313  [show with all ESTs]
    Unigene Representative Sequence: NM_001251830
    10 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000237623(uc003hra.3 uc003hrb.3 uc003hrc.3 uc003hrd.3)
    ENST00000395080 ENST00000504310 ENST00000513981 ENST00000509334 ENST00000508233
    ENST00000360804 ENST00000509659(uc011cde.2) ENST00000508002 ENST00000505146


    miRNA
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    8 QIAGEN miScript miRNA Assays for microRNAs that regulate SPP1:
    hsa-miR-181c hsa-miR-3065-5p hsa-miR-33b hsa-miR-181a hsa-miR-181d hsa-miR-33a hsa-miR-181b hsa-miR-4262
    SwitchGear 3'UTR luciferase reporter plasmidSPP1 3' UTR sequence
    Inhib. RNA
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    Browse for Gene Knock-down Tools from EMD Millipore
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    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat SPP1
    Clone
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    OriGene clones in human, mouse for SPP1 (see all 21)
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    GenScript: all cDNA clones in your preferred vector (see all 3): SPP1 (NM_001040058)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for SPP1
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat SPP1
    Sirion Biotech Customized lentivirus for stable overexpression of SPP1 
                         Customized lentivirus expression plasmids for stable overexpression of SPP1 
    Primer
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    OriGene qPCR primer pairs and template standards for SPP1
    OriGene qSTAR qPCR primer pairs in human, mouse for SPP1
    SABiosciences RT2 qPCR Primer Assay in human, mouse, rat SPP1
      QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat SPP1
      Search QIAGEN QuantiFast Probe-based Assays in human, mouse, rat SPP1

    Additional mRNA sequence: 

    AB019562.1 AB209987.1 AB469789.1 AF052124.1 AK057738.1 AK075463.1 AK290090.1 AK290104.1 
    AK295491.1 AK296035.1 AK315461.1 AY956318.1 BC007016.1 BC017387.1 BC022844.1 BC093033.1 
    BX648003.1 D28759.1 D28760.1 D28761.1 DQ839491.1 DQ846870.1 DQ846871.1 HQ874460.1 
    HQ874461.1 J04765.1 M83248.1 X13694.1 

    24/50 DOTS entries (see all 50):

    DT.100889418  DT.99982600  DT.95324972  DT.92471676  DT.100889364  DT.454110  DT.91739764  DT.91690113 
    DT.92471713  DT.92410290  DT.121247185  DT.100889395  DT.100889393  DT.100889376  DT.95299295  DT.435134 
    DT.100889441  DT.95311246  DT.100889421  DT.92471710  DT.121247173  DT.121247212  DT.121247266  DT.100889380 

    24/1338 AceView cDNA sequences (see all 1338):

    CR602328 CR600714 BX439411 CA843275 BQ775873 BE349276 CR626471 BC007016 
    CR612712 CR612096 H88773 BQ638828 BU785520 BU619656 CR610632 H88774 
    AA345192 CR594979 AA336664 W92752 BQ576028 AL542081 CR610546 CR610252 

    GeneLoc Exon Structure

    5/6 Alternative Splicing Database (ASD) splice patterns (SP) for SPP1 (see all 6)    About this scheme

    ExUns: 1a · 1b ^ 2 ^ 3 ^ 4a · 4b ^ 5 ^ 6a · 6b ^ 7 ^ 8a · 8b · 8c ^ 9a · 9b
    SP1:                          -     -                 -                                       
    SP2:                          -     -                 -                                       
    SP3:                                -                 -                                       
    SP4:                                                  -                                       
    SP5:                          -     -                                                         


    ECgene alternative splicing isoforms for SPP1

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    SPP1 expression in normal human tissues (normalized intensities)      SPP1 embryonic expression: see
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: AATAGAAATT
    SPP1 Expression
    About this image


    SPP1 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database 
     5/28 selected tissues (see all 28) fully expand
     
     Bone (Muscoskeletal System)    fully expand to see all 78 entries
             Hypertrophic Chondrocytes Stylopod Growth Plate
             Zeugopod Growth Plate
             HyStem+BMP4-induced 4D20.8 cells
             Human Vertebral Mesenchymal Stem Cells (HVMSC)   
     
     Cartilage (Muscoskeletal System)    fully expand to see all 35 entries
             Terminal Chondrocytes Temporomandibular Joint
             Chondrocranium
             HyStem+BMP4-induced 4D20.8 cells
     
     Kidney (Urinary System)    fully expand to see all 20 entries
             Proximal Tubule Cells Proximal Tubule
             Metanephros
             Human Renal Mesangial Cells (HRMC)   
             visceral organ/metanephros/renal medulla   
     
     Brain (Nervous System)    fully expand to see all 10 entries
             Adult Dopaminergic Neurons Substantia Nigra pars Compacta
             Hypothalamus
             Human Hippocampal Astrocytes   
             Trigeminal Ganglion   
     
     Inner Cell Mass (Early Embryonic Tissues)    fully expand to see all 7 entries
             Inner Cell Mass Cells Inner Cell Mass
             Sox17-GFP genetically modified stem cells

    See SPP1 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for SPP1

    SOURCE GeneReport for Unigene cluster: Hs.313

    UniProtKB/Swiss-Prot: OSTP_HUMAN, P10451
    Tissue specificity: Bone. Found in plasma

        SABiosciences Expression via Pathway-Focused PCR Arrays including SPP1 (see all 18): 
              Lung Cancer in human mouse rat
              Extracellular Matrix & Adhesion Molecules in human mouse rat
              Antiviral Response in human mouse rat
              T-cell & B-cell Activation in human mouse rat
              Estrogen Receptor Signaling in human mouse rat

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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of chordates.

    Orthologs for SPP1 gene from 3/9 species (see all 9)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Spp11 , 5 secreted phosphoprotein 11, 5 73.93(n)1
    68.05(a)1
      5 (50.68 cM)5
    207501  NM_001204201.11  NP_001191130.11 
     1044351185 
    chicken
    (Gallus gallus)
    Aves SPP16
    osteopontin precursor
    30(a)
    1 ↔ 1
    4(45074192-45077215)
    lizard
    (Anolis carolinensis)
    Reptilia SPP16
    Uncharacterized protein
    29(a)
    1 ↔ 1
    5(45557752-45565946)


    ENSEMBL Gene Tree for SPP1 (if available)
    TreeFam Gene Tree for SPP1 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for SPP1 gene
    1 SIMAP similar gene for SPP1 using alignment to 2 protein entries:     OSTP_HUMAN (see all proteins):
    opn

    SPP1 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/295 SNPs in SPP1 are shown (see all 295)    About this table     
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 4 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs454739981,2
    F--84649078(+) TCCAAT/CGACCA 10 /N syn11Minor allele frequency- C:0.29NA 48
    rs1147085541,2
    C--88703459(+) GAAAAC/TTGGTA 5 -- us2k10--------
    rs1925043641,2
    --88703474(+) CAGATC/TGTGGT 5 -- us2k10--------
    rs1392812151,2
    --88703487(+) GAAATA/CTTTTT 5 -- us2k10--------
    rs1808278991,2
    --88703546(+) TCCTGC/TAAAGG 5 -- us2k10--------
    rs1488762171,2
    C--88703549(+) GTAAA-/GGGGTC 5 -- us2k10--------
    rs290015111,2
    C,F--88703666(+) TCCTGT/CGGAAA 5 -- us2k11Minor allele frequency- C:0.02NA 120
    rs27281271,2
    C,F,A--88703694(+) TTTTCA/GGACTT 5 -- us2k115Minor allele frequency- N:0.01EA NA WA CSA 589
    rs1144274321,2
    F--88703849(+) TTATTA/CAAATG 5 -- us2k11Minor allele frequency- C:0.01WA 118
    rs1861287651,2
    --88703941(+) ATGTTC/TATGAA 5 -- us2k10--------

    HapMap Linkage Disequilibrium report for SPP1 (88896802 - 88904563 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 2 variations for SPP1:    About this table     
    Variant IDTypeSubtypePubMed ID
    esv2665331CNV Deletion23128226
    nsv7364OTHER Inversion18451855


    Human Gene Mutation Database (HGMD): SPP1
    SABiosciences Cancer Mutation PCR Assays
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    DNA2.0 Custom Variant and Variant Library Synthesis for SPP1

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section
    OMIM gene information: 166490    OMIM disorders: --

    20/220 diseases for SPP1 (see all 220):    About MalaCards
    osteofibrous dysplasia    papillary cystadenocarcinoma    coronary restenosis    calcific tendinitis
    chorioangioma    conjunctival disease    tympanosclerosis    dentin dysplasia
    dentin dysplasia, type ii    devic disease    dentinogenesis imperfecta    calciphylaxis
    malignant pleural mesothelioma    autoimmune myocarditis    malignant mesothelioma    atypical teratoid rhabdoid tumor
    rhabdoid tumor    hyperphosphatemia    pigmented villonodular synovitis    autoimmune lymphoproliferative syndrome

    7 diseases from the University of Copenhagen DISEASES database for SPP1:
    Cancer     Kidney disease     Osteoporosis     Atherosclerosis
    Calcinosis     Heart disease     Benign mesothelioma

    SPP1 for disorders           About GeneDecksing


    Congresses - knowledge worth sharing:  
    Alzheimer's & Parkinson's Diseases Congress (ADPD) 18 - 22 March 2015

    10/98 Novoseek inferred disease relationships for SPP1 gene (see all 98)    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    calcification 77.6 339 10766759 (10), 15041047 (7), 19902145 (6), 15820201 (6) (see all 99)
    metastasis 64.3 485 16831281 (6), 11315992 (6), 18972404 (5), 9637769 (4) (see all 99)
    dentinogenesis imperfecta 63.3 2 9541230 (1), 7665163 (1)
    renal stone 62.9 44 8619372 (5), 8761944 (4), 7571189 (3), 7735136 (3) (see all 17)
    urinary stones 59.8 59 8325891 (4), 10929424 (4), 9365840 (3), 1575754 (2) (see all 25)
    dentin dysplasia 58.1 1 9541230 (1)
    osteosarcoma 56.5 28 12404059 (5), 17022822 (4), 14975209 (2), 14994076 (2) (see all 13)
    tumors 54.6 841 9761120 (9), 8080043 (8), 15355566 (7), 20377868 (7) (see all 99)
    lymphatic metastasis 49.3 13 16880782 (2), 17148500 (2), 16816376 (2), 17181739 (1) (see all 6)
    pleural malignant mesothelioma 48.9 14 17493236 (2), 19528482 (2), 17504993 (2), 16221779 (2) (see all 8)

    Genetic Association Database (GAD): SPP1
    Human Genome Epidemiology (HuGE) Navigator: SPP1 (56 documents)

    Export disorders for SPP1 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for SPP1 gene, integrated from 9 sources (see all 1986):
    (articles sorted by number of sources associating them with SPP1)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Molecular cloning and sequencing of cDNA encoding urinary stone protein, which is identical to osteopontin. (PubMed id 1575754)1, 2, 3, 9 Kohri K.... Kurita T. (1992)
    2. The implication of osteopontin (OPN) expression and g enetic polymorphisms of OPN promoter in oral carcinogenesis. (PubMed id 20219412)1, 4, 9 Chiu Y.W....Kao S.Y. (2010)
    3. Association between osteopontin and human abdominal aortic aneurysm. (PubMed id 17170369)1, 4, 9 Golledge J....Norman P.E. (2007)
    4. Overexpression of osteopontin in rheumatoid synovial mononuclear cells is associated with joint inflammation, not with genetic polymorphism. (PubMed id 15742430)1, 4, 9 Xu G....Zhang J. (2005)
    5. Expression of osteopontin in human glioma. Its correlation with the malignancy. (PubMed id 7837791)1, 2, 9 Saitoh Y.... Ushio Y. (1995)
    6. A polymorphism of the osteopontin gene is related to urinary calcium stones. (PubMed id 16145474)1, 4, 9 Gao B....Kohri K. (2005)
    7. Matrix Gla protein and osteopontin genetic associations with coronary artery calcification and bone density: the CARDIA study. (PubMed id 15744522)1, 4, 9 Taylor B.C....Sidney S. (2005)
    8. The impact of osteopontin promoter polymorphisms on t he risk of calcium urolithiasis. (PubMed id 20144595)1, 4, 9 Liu C.C....Wu M.T. (2010)
    9. Haplotype analysis revealed a genetic influence of osteopontin on large artery atherosclerosis. (PubMed id 18317949)1, 4, 9 Kim Y. and Lee C. (2008)
    10. Osteopontin polymorphisms and disease course in multiple sclerosis. (PubMed id 12761568)1, 4, 9 Caillier S....Oksenberg J.R. (2003)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 6696 HGNC: 11255 AceView: SPP1 Ensembl:ENSG00000118785 euGenes: HUgn6696
    ECgene: SPP1 Kegg: 6696 H-InvDB: SPP1

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for SPP1 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for SPP1 Genetics and Cytogenetics in Oncology and Haematology
    Wikipedia http://en.wikipedia.org/wiki/Osteopontin

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for SPP1 gene:
    Search GeneIP for patents involving SPP1

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0 and Sirion Biotech, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Sirion Biotech, Cell lines from GenScript, and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences, In Situ Hybridization Assays from
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    About This Section

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