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SPP1 Gene

protein-coding   GIFtS: 71
GCID: GC04P088896

Secreted Phosphoprotein 1

(Previous names: osteopontin, bone sialoprotein I)
(Previous symbols: BNSP, OPN)
  See related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Secreted Phosphoprotein 11 2 3     nephropontin2
OPN1 2 3 5     Osteopontin/Immunoglobulin Alpha 1 Heavy Chain Constant Region Fusion
Protein2
BNSP1 2 3     Secreted Phosphoprotein 1 (Osteopontin, Bone Sialoprotein I, Early
T-Lymphocyte Activation 1)2
osteopontin1 2     SPP1/CALPHA1 Fusion2
Early T-Lymphocyte Activation 11 2     uropontin2
Urinary Stone Protein2 3     Nephropontin3
Bone Sialoprotein I1     SPP-13
BSPI2     Uropontin3
ETA-12     Bone Sialoprotein 13

External Ids:    HGNC: 112551   Entrez Gene: 66962   Ensembl: ENSG000001187857   OMIM: 1664905   UniProtKB: P104513   

Export aliases for SPP1 gene to outside databases

Previous GC identifers: GC04P089054 GC04P089198 GC04P089355 GC04P089253 GC04P089115 GC04P084643


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for SPP1 Gene:
The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The
encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is
found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine
that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding
different isoforms have been found for this gene. (provided by RefSeq, Oct 2011)

GeneCards Summary for SPP1 Gene:
SPP1 (secreted phosphoprotein 1) is a protein-coding gene. Diseases associated with SPP1 include coronary restenosis, and tendinitis. GO annotations related to this gene include extracellular matrix binding and cytokine activity.

UniProtKB/Swiss-Prot: OSTP_HUMAN, P10451
Function: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably
important to cell-matrix interaction
Function: Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing
production of interleukin-10 and is essential in the pathway that leads to type I immunity (By similarity)

Gene Wiki entry for SPP1 (Osteopontin) Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence:
NC_000004.12  NC_018915.2  NT_016354.20  
Regulatory elements:
   Regulatory transcription factor binding sites in the SPP1 gene promoter:
         AML1a   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 3): SPP1 promoter sequence
   Search Chromatin IP Primers for SPP1

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat SPP1


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 4q22.1   Ensembl cytogenetic band:  4q22.1   HGNC cytogenetic band: 4q22.1

SPP1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
SPP1 gene location

GeneLoc information about chromosome 4         GeneLoc Exon Structure

GeneLoc location for GC04P088896:  view genomic region     (about GC identifiers)

Start:
88,896,802 bp from pter      End:
88,904,563 bp from pter
Size:
7,762 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: OSTP_HUMAN, P10451 (See protein sequence)
Recommended Name: Osteopontin precursor  
Size: 314 amino acids; 35423 Da
Subunit: Ligand for integrin alpha-V/beta-3
2 PDB 3D structures from and Proteopedia for SPP1:
3CXD (3D)        3DSF (3D)    
Secondary accessions: B2RDA1 Q15681 Q15682 Q15683 Q4W597 Q567T5 Q8NBK2 Q96IZ1
Alternative splicing: 5 isoforms:  P10451-1   P10451-2   P10451-3   P10451-4   P10451-5   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for SPP1: NX_P10451

Explore proteomics data for SPP1 at MOPED

Post-translational modifications: 

  • Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif1
  • N- and O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-621
  • Glycosylation2 at Asn79, Asn106, Thr134, Thr138, Thr143, Thr147, Thr152
  • Modification sites at PhosphoSitePlus

  • See SPP1 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (5 alternative transcripts): 
    NP_000573.1  NP_001035147.1  NP_001035149.1  NP_001238758.1  NP_001238759.1  

    ENSEMBL proteins: 
     ENSP00000237623   ENSP00000378517   ENSP00000422973   ENSP00000354042  
    Reactome Protein details: P10451

    SPP1 Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
    R&D Systems Recombinant & Natural Proteins for SPP1 (Osteopontin/OPN)
    Browse recombinant and purified proteins available from Enzo Life Sciences
    OriGene Purified Proteins for SPP1
    OriGene Protein Over-expression Lysate for SPP1
    OriGene MassSpec for SPP1
    OriGene Custom Protein Services for SPP1
    GenScript Custom Purified and Recombinant Proteins Services for SPP1
    Novus Biologicals SPP1 Proteins
    Novus Biologicals SPP1 Lysates
    Browse Sino Biological Recombinant Proteins
    Sino Biological Cell Lysate for SPP1
    ProSpec Recombinant Protein for SPP1
    Browse Proteins at Cloud-Clone Corp.

    SPP1 Antibody Products:

    EMD Millipore Mono- and Polyclonal Antibodies for the study of SPP1
    R&D Systems Antibodies for SPP1 (Osteopontin/OPN)
    OriGene Antibodies for SPP1
    OriGene Custom Antibody Services for SPP1
    Novus Biologicals SPP1 Antibodies
    Abcam antibodies for SPP1
    Browse Antibodies at Cloud-Clone Corp.
    ThermoFisher Antibody for SPP1
    LSBio Antibodies in human, mouse, rat for SPP1

    SPP1 Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for SPP1
    R&D Systems ELISAs for SPP1 (Osteopontin/OPN)         (see all)
    GenScript Custom Assay Services for SPP1
    Enzo Life Sciences assays for SPP1
    Browse ELISAs at Cloud-Clone Corp.
    Browse CLIAs at Cloud-Clone Corp.


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    HGNC Gene Families:
    ENDOLIG: Endogenous ligands

    2 InterPro protein domains:
     IPR002038 Osteopontin
     IPR019841 Osteopontin_CS

    Graphical View of Domain Structure for InterPro Entry P10451

    ProtoNet protein and cluster: P10451

    1 Blocks protein domain: IPB002038 Osteopontin

    UniProtKB/Swiss-Prot: OSTP_HUMAN, P10451
    Similarity: Belongs to the osteopontin family


    SPP1 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: OSTP_HUMAN, P10451
    Function: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably
    important to cell-matrix interaction
    Function: Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing
    production of interleukin-10 and is essential in the pathway that leads to type I immunity (By similarity)

         Genatlas biochemistry entry for SPP1:
    secreted phosphoprotein 1 (osteopontin),regulated by CBFA1 and ETS1 in the skeletal tissue

         Gene Ontology (GO): 2 molecular function terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005125cytokine activity IEA--
    GO:0050840extracellular matrix binding IEA--
         
    SPP1 for ontologies           About GeneDecksing


    Phenotypes:
         2 GenomeRNAi human phenotypes for SPP1:
     Decreased CXCL12 induced migra  Decreased viability with pacli 

         Selected MGI mutant phenotypes (inferred from 4 alleles(MGI details for Spp1) (see all 17):
     behavior/neurological  cardiovascular system  cellular  growth/size/body  hematopoietic system 
     homeostasis/metabolism  immune system  integument  liver/biliary system  mortality/aging 
     muscle  nervous system  renal/urinary system  reproductive system  respiratory system 

    SPP1 for phenotypes           About GeneDecksing

    Animal Models:
         MGI mouse knock-outs for SPP1: Spp1tm1Blh Spp1tm1Rit

       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for SPP1
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for SPP1

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for SPP1
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for SPP1

    miRNA
    Products:
        
    miRTarBase miRNAs that target SPP1:
    hsa-mir-146a-5p (MIRT021279), hsa-mir-335-5p (MIRT016866), hsa-mir-299-5p (MIRT004565)

    Block miRNA regulation of human, mouse, rat SPP1 using miScript Target Protectors
    8 qRT-PCR Assays for microRNAs that regulate SPP1:
    hsa-miR-181c hsa-miR-3065-5p hsa-miR-33b hsa-miR-181a hsa-miR-181d hsa-miR-33a hsa-miR-181b hsa-miR-4262
    SwitchGear 3'UTR luciferase reporter plasmidSPP1 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for SPP1
    Predesigned siRNA for gene silencing in human, mouse, rat SPP1

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for SPP1

    Clone
    Products:
         
    OriGene clones in human, mouse for SPP1 (see all 19)
    OriGene ORF clones in mouse, rat for SPP1
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 3): SPP1 (NM_001040058)
    Sino Biological Human cDNA Clone for SPP1
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for SPP1
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat SPP1

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for SPP1
    ESI BIO PureStem Progenitors for SPP1: 
    PureStem 7SMOO32, NCr-fac & Meso Progenitor, PureStem SK11, NCr-fac & Meso-prx Progenitor
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for SPP1


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    OSTP_HUMAN, P10451: Secreted
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    extracellular5
    cytoskeleton2
    nucleus2
    plasma membrane2
    cytosol1
    golgi apparatus1

    Gene Ontology (GO): Selected cellular component terms (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005576extracellular region TAS--
    GO:0005615extracellular space IDA15516325
    GO:0005737cytoplasm ----
    GO:0031988membrane-bounded vesicle IEA--
    GO:0042995cell projection IEA--

    SPP1 for ontologies           About GeneDecksing


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for SPP1 About   (see all 23)  
    See pathways by source

    SuperPathContained pathways About
    1Toll-like receptor signaling pathway
    Toll-like receptor signaling pathway0.94
    Regulation of toll-like receptor signaling pathway0.93
    Toll-like receptor signaling pathway0.94
    2Focal adhesion
    Focal adhesion0.65
    ECM-receptor interaction0.31
    Focal Adhesion0.65
    3Integrin Pathway
    FAK1 Signaling0.67
    UPA-UPAR Pathway0.56
    Integrin Pathway0.67
    Inhibition of Angiogenesis by TSP10.50
    GnRH Signaling0.56
    Transendothelial Migration of Leukocytes0.38
    4ERK Signaling
    Rho Family GTPases0.61
    Molecular Mechanisms of Cancer0.51
    ERK Signaling0.61
    ILK Signaling0.49
    MAPK Signaling0.58
    5Phospholipase-C Pathway
    Phospholipase-C Pathway0.56
    PTEN Pathway0.56

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    Selected Downloadable PowerPoint Slides of GeneGlobe Pathway Central Maps for SPP1 (see all 15)
        MAPK Signaling
    Molecular Mechanisms of Cancer
    PTEN Pathway
    PI3K Signaling in B-Lymphocyte
    Transendothelial Migration of Leukocytes

    2 GeneGo (Thomson Reuters) Pathways for SPP1
        Development Hedgehog and PTH signaling pathways in bone and cartilage development
    Transcription Role of VDR in regulation of genes involved in osteoporosis

    Selected BioSystems Pathways for SPP1 (see all 12)
        Osteopontin Signaling
    Focal Adhesion
    TGF Beta Signaling Pathway
    BDNF signaling pathway
    Endochondral Ossification


    3 Reactome Pathways for SPP1
        Integrin cell surface interactions
    Signaling by PDGF
    Degradation of the extracellular matrix


    4 Kegg Pathways  (Kegg details for SPP1):
        PI3K-Akt signaling pathway
    Focal adhesion
    ECM-receptor interaction
    Toll-like receptor signaling pathway


    SPP1 for pathways           About GeneDecksing

        Pathway & Disease-focused RT2 Profiler PCR Arrays including SPP1 (see all 18): 
              Lung Cancer in human mouse rat
              Extracellular Matrix & Adhesion Molecules in human mouse rat
              Antiviral Response in human mouse rat
              T-cell & B-cell Activation in human mouse rat
              Estrogen Receptor Signaling in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for SPP1

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for SPP1 (P104512, 3 ENSP000003785174) via UniProtKB, MINT, STRING, and/or I2D (see all 48)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    ABCF3Q9NUQ82, 3MINT-65157 I2D: score=4 
    UBQLN4Q9NRR52, 3, ENSP000003572924MINT-2863322 MINT-2863341 I2D: score=4 STRING: ENSP00000357292
    SGTAO437652, 3, ENSP000002215664MINT-66220 I2D: score=5 STRING: ENSP00000221566
    CTNNBL1Q8WYA62, 3, ENSP000003550504MINT-67203 I2D: score=5 STRING: ENSP00000355050
    PDLIM7Q9NR122, 3, ENSP000003480994MINT-66700 I2D: score=5 STRING: ENSP00000348099
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 17):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001503ossification ----
    GO:0001649osteoblast differentiation IEA--
    GO:0006954inflammatory response IEA--
    GO:0007155cell adhesion IEA--
    GO:0007566embryo implantation TAS16720713

    SPP1 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for SPP1 (OSTP)

    Selected Novoseek inferred chemical compound relationships for SPP1 gene (see all 110)    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    calcium oxalate 78.9 137 19581305 (5), 10461108 (5), 12706004 (4), 16104927 (4) (see all 44)
    grgds 76.3 42 7522656 (6), 8972713 (4), 1421573 (2), 11787071 (1) (see all 17)
    alizarin 75.8 6 15165454 (1), 15271969 (1), 20413153 (1), 15956138 (1) (see all 6)
    hydroxyapatite 74.7 143 16007483 (9), 8010953 (4), 7615968 (4), 16011845 (4) (see all 58)
    1,25 dihydroxy vitamin d3 60.5 12 10404392 (1), 11834737 (1), 7571189 (1), 8900396 (1) (see all 12)
    lm 609 60.4 2 10726209 (1), 7522656 (1)
    nimorazole 59.5 2 16198981 (2)
    calcium 52.6 169 16145474 (4), 10460892 (4), 7988430 (4), 20144595 (3) (see all 91)
    titanium 52 14 20155835 (2), 18983324 (2), 17437306 (2), 11173803 (1) (see all 6)
    vitamin d 50 43 19246678 (2), 7708050 (1), 9556070 (1), 1665202 (1) (see all 28)



    SPP1 for compounds           About GeneDecksing



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
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    REFSEQ mRNAs for SPP1 gene (5 alternative transcripts): 
    NM_000582.2  NM_001040058.1  NM_001040060.1  NM_001251829.1  NM_001251830.1  

    Unigene Cluster for SPP1:

    Secreted phosphoprotein 1
    Hs.313  [show with all ESTs]
    Unigene Representative Sequence: NM_001251830
    10 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000237623(uc003hra.3 uc003hrb.3 uc003hrc.3 uc003hrd.3)
    ENST00000395080 ENST00000504310 ENST00000513981 ENST00000509334 ENST00000508233
    ENST00000360804 ENST00000509659(uc011cde.2) ENST00000508002 ENST00000505146

    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat SPP1 using miScript Target Protectors
    8 qRT-PCR Assays for microRNAs that regulate SPP1:
    hsa-miR-181c hsa-miR-3065-5p hsa-miR-33b hsa-miR-181a hsa-miR-181d hsa-miR-33a hsa-miR-181b hsa-miR-4262
    SwitchGear 3'UTR luciferase reporter plasmidSPP1 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for SPP1
    Predesigned siRNA for gene silencing in human, mouse, rat SPP1
    Clone
    Products:
         
    OriGene clones in human, mouse for SPP1 (see all 19)
    OriGene ORF clones in mouse, rat for SPP1
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 3): SPP1 (NM_001040058)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for SPP1
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat SPP1
    Primer
    Products:
        
    OriGene qPCR primer pairs and template standards for SPP1
    OriGene qSTAR qPCR primer pairs in human, mouse for SPP1
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat SPP1
      QuantiTect SYBR Green Assays in human, mouse, rat SPP1
      Search QuantiFast Probe-based Assays in human, mouse, rat SPP1

    Additional mRNA sequence: 

    AB019562.1 AB209987.1 AB469789.1 AF052124.1 AK057738.1 AK075463.1 AK290090.1 AK290104.1 
    AK295491.1 AK296035.1 AK315461.1 AY956318.1 BC007016.1 BC017387.1 BC022844.1 BC093033.1 
    BX648003.1 D28759.1 D28760.1 D28761.1 DQ839491.1 DQ846870.1 DQ846871.1 HQ874460.1 
    HQ874461.1 J04765.1 M83248.1 X13694.1 

    Selected DOTS entries (see all 50):

    DT.100889418  DT.99982600  DT.95324972  DT.92471676  DT.100889364  DT.454110  DT.91739764  DT.91690113 
    DT.92471713  DT.92410290  DT.121247185  DT.100889395  DT.100889393  DT.100889376  DT.95299295  DT.435134 
    DT.100889441  DT.95311246  DT.100889421  DT.92471710  DT.121247173  DT.121247212  DT.121247266  DT.100889380 

    Selected AceView cDNA sequences (see all 1338):

    BQ576028 CR602900 BG219424 CR608820 CR600682 BI858661 CA428029 CR594254 
    CR599125 BX439411 AA369511 CR625747 BI861014 CR624945 AI623991 CR601143 
    X13694 CR612096 CR610677 AL541101 BG164584 BU736483 CR626718 BQ637236 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for SPP1 (see all 6)    About this scheme

    ExUns: 1a · 1b ^ 2 ^ 3 ^ 4a · 4b ^ 5 ^ 6a · 6b ^ 7 ^ 8a · 8b · 8c ^ 9a · 9b
    SP1:                          -     -                 -                                       
    SP2:                          -     -                 -                                       
    SP3:                                -                 -                                       
    SP4:                                                  -                                       
    SP5:                          -     -                                                         


    ECgene alternative splicing isoforms for SPP1

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

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    SPP1 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: AATAGAAATT
    SPP1 Expression
    About this image


    SPP1 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 27) fully expand
     
     Bone (Muscoskeletal System)    fully expand to see all 64 entries
             Endochondral Osteoblasts Rostral Endochondral Facial Bones
             Zeugopod Growth Plate
             HyStem+TGFbeta3+GDF5-induced SK11 cells
     
     Cartilage (Muscoskeletal System)    fully expand to see all 30 entries
             Terminal Chondrocytes Temporomandibular Joint
             Chondrocranium
             HyStem+TGFbeta3+GDF5-induced SK11 cells
     
     Kidney (Urinary System)    fully expand to see all 10 entries
             Proximal Tubule Cells Proximal Tubule
             Metanephros
     
     Pancreas (Endocrine System)    fully expand to see all 3 entries
             Bipotent Endocrine/Duct Progenitor Cells Dorsal Pancreatic Bud
             Islets of Langerhans
     
     Inner Cell Mass (Early Embryonic Tissues)    fully expand to see all 8 entries
             Inner Cell Mass Cells Inner Cell Mass
             Human embryonic stem cells (family)
    SPP1 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    SPP1 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.313

    UniProtKB/Swiss-Prot: OSTP_HUMAN, P10451
    Tissue specificity: Bone. Found in plasma

        Pathway & Disease-focused RT2 Profiler PCR Arrays including SPP1 (see all 18): 
              Lung Cancer in human mouse rat
              Extracellular Matrix & Adhesion Molecules in human mouse rat
              Antiviral Response in human mouse rat
              T-cell & B-cell Activation in human mouse rat
              Estrogen Receptor Signaling in human mouse rat

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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of human and mouse.

    Orthologs for SPP1 gene from Selected species (see all 7)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Spp11 , 5 secreted phosphoprotein 11, 5 72.77(n)1
    66.1(a)1
      5 (50.68 cM)5
    207501  NM_001204201.11  NP_001191130.11 
     1044351185 


    ENSEMBL Gene Tree for SPP1 (if available)
    TreeFam Gene Tree for SPP1 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for SPP1 gene
    1 SIMAP similar gene for SPP1 using alignment to 2 protein entries:     OSTP_HUMAN (see all proteins):
    opn

    SPP1 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for SPP1 (see all 295)    About this table    
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 4 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs454739981,2
    F--84649078(+) TCCAAT/CGACCA 10 /N syn11Minor allele frequency- C:0.29NA 48
    rs1147085541,2
    C--88703459(+) GAAAAC/TTGGTA 5 -- us2k10--------
    rs1925043641,2
    --88703474(+) CAGATC/TGTGGT 5 -- us2k10--------
    rs1392812151,2
    --88703487(+) GAAATA/CTTTTT 5 -- us2k10--------
    rs1808278991,2
    --88703546(+) TCCTGC/TAAAGG 5 -- us2k10--------
    rs1488762171,2
    C--88703549(+) GTAAA-/GGGGTC 5 -- us2k10--------
    rs290015111,2
    C,F--88703666(+) TCCTGT/CGGAAA 5 -- us2k11Minor allele frequency- C:0.02NA 120
    rs27281271,2
    C,F,A--88703694(+) TTTTCA/GGACTT 5 -- us2k115Minor allele frequency- N:0.01EA NA WA CSA 589
    rs1144274321,2
    F--88703849(+) TTATTA/CAAATG 5 -- us2k11Minor allele frequency- C:0.01WA 118
    rs1861287651,2
    --88703941(+) ATGTTC/TATGAA 5 -- us2k10--------

    HapMap Linkage Disequilibrium report for SPP1 (88896802 - 88904563 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 2 variations for SPP1:    About this table    
    Variant IDTypeSubtypePubMed ID
    esv2665331CNV Deletion23128226
    nsv7364OTHER Inversion18451855

    Human Gene Mutation Database (HGMD): SPP1
    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing SPP1
    DNA2.0 Custom Variant and Variant Library Synthesis for SPP1

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 166490    OMIM disorders: --

    Selected diseases for SPP1 (see all 236):    
    About MalaCards
    coronary restenosis    tendinitis    calcific tendinitis    papillary cystadenocarcinoma
    pediatric systemic lupus erythematosus    osteofibrous dysplasia    chorioangioma    conjunctival disease
    dentin dysplasia    calciphylaxis    tympanosclerosis    dentinogenesis imperfecta
    dentin dysplasia, type ii    devic disease    retroperitoneal fibrosis    autoimmune myocarditis
    calcinosis    hyperphosphatemia    atypical teratoid rhabdoid tumor    aortic valve disease

    7 diseases from the University of Copenhagen DISEASES database for SPP1:
    Cancer     Kidney disease     Osteoporosis     Atherosclerosis
    Calcinosis     Heart disease     Benign mesothelioma

    SPP1 for disorders           About GeneDecksing

    Selected Novoseek inferred disease relationships for SPP1 gene (see all 98)    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    calcification 77.6 339 10766759 (10), 15041047 (7), 19902145 (6), 15820201 (6) (see all 99)
    metastasis 64.3 485 16831281 (6), 11315992 (6), 18972404 (5), 9637769 (4) (see all 99)
    dentinogenesis imperfecta 63.3 2 9541230 (1), 7665163 (1)
    renal stone 62.9 44 8619372 (5), 8761944 (4), 7571189 (3), 7735136 (3) (see all 17)
    urinary stones 59.8 59 8325891 (4), 10929424 (4), 9365840 (3), 1575754 (2) (see all 25)
    dentin dysplasia 58.1 1 9541230 (1)
    osteosarcoma 56.5 28 12404059 (5), 17022822 (4), 14975209 (2), 14994076 (2) (see all 13)
    tumors 54.6 841 9761120 (9), 8080043 (8), 15355566 (7), 20377868 (7) (see all 99)
    lymphatic metastasis 49.3 13 16880782 (2), 17148500 (2), 16816376 (2), 17181739 (1) (see all 6)
    pleural malignant mesothelioma 48.9 14 17493236 (2), 19528482 (2), 17504993 (2), 16221779 (2) (see all 8)

    Genetic Association Database (GAD): SPP1
    Human Genome Epidemiology (HuGE) Navigator: SPP1 (56 documents)

    Export disorders for SPP1 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for SPP1 gene, integrated from 10 sources (see all 2021):
    (articles sorted by number of sources associating them with SPP1)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Molecular cloning and sequencing of cDNA encoding urinary stone protein, which is identical to osteopontin. (PubMed id 1575754)1, 2, 3, 9 Kohri K.... Kurita T. (Biochem. Biophys. Res. Commun. 1992)
    2. The implication of osteopontin (OPN) expression and genetic polymorphisms of OPN promoter in oral carcinogenesis. (PubMed id 20219412)1, 4, 9 Chiu Y.W....Kao S.Y. (Oral Oncol. 2010)
    3. Association between osteopontin and human abdominal aortic aneurysm. (PubMed id 17170369)1, 4, 9 Golledge J....Norman P.E. (Arterioscler. Thromb. Vasc. Biol. 2007)
    4. Overexpression of osteopontin in rheumatoid synovial mononuclear cells is associated with joint inflammation, not with genetic polymorphism. (PubMed id 15742430)1, 4, 9 Xu G....Zhang J. (J. Rheumatol. 2005)
    5. Expression of osteopontin in human glioma. Its correlation with the malignancy. (PubMed id 7837791)1, 2, 9 Saitoh Y.... Ushio Y. (Lab. Invest. 1995)
    6. A polymorphism of the osteopontin gene is related to urinary calcium stones. (PubMed id 16145474)1, 4, 9 Gao B....Kohri K. (J. Urol. 2005)
    7. Matrix Gla protein and osteopontin genetic associations with coronary artery calcification and bone density: the CARDIA study. (PubMed id 15744522)1, 4, 9 Taylor B.C....Sidney S. (Hum. Genet. 2005)
    8. The impact of osteopontin promoter polymorphisms on the risk of calcium urolithiasis. (PubMed id 20144595)1, 4, 9 Liu C.C....Wu M.T. ( international journal of clinical chemistry 2010)
    9. Haplotype analysis revealed a genetic influence of osteopontin on large artery atherosclerosis. (PubMed id 18317949)1, 4, 9 Kim Y. and Lee C. (J. Biomed. Sci. 2008)
    10. Osteopontin polymorphisms and disease course in multiple sclerosis. (PubMed id 12761568)1, 4, 9 Caillier S....Oksenberg J.R. (Genes Immun. 2003)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 6696 HGNC: 11255 AceView: SPP1 Ensembl:ENSG00000118785 euGenes: HUgn6696
    ECgene: SPP1 Kegg: 6696 H-InvDB: SPP1

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for SPP1 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for SPP1 Genetics and Cytogenetics in Oncology and Haematology
    Wikipedia http://en.wikipedia.org/wiki/Osteopontin

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for SPP1 gene:
    Search GeneIP for patents involving SPP1

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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