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SPOCK3 Gene

protein-coding   GIFtS: 54

GC04M167891
sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 3
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
HSAJ1454 2
TES-3 2
TICN3 3
testican-3 1
Descriptions
SPARC/osteonectin, CWCV, and Kazal-like domains
proteoglycan 3 3
sparc/osteonectin, cwcv and kazal-like domains proteoglycan
(testican) 3 2
testican 3 2
External Ids
HGNC: 135651
Entrez Gene: 508592
UniProtKB: Q9BQ163
Ensembl: ENSG000001961047
Search outside databases for aliases for SPOCK3 gene

Previous GC identifers: GC04M168064 GC04M168238 GC04M168350 GC04M168029

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for SPOCK3:
Proteoglycans, which consist of a core protein and covalently linked glycosaminoglycans, are
components of the extracellular matrix. SPOCK3 encodes a member of a novel Ca(2+)-binding
proteoglycan family.[supplied by OMIM]

UniProtKB/Swiss-Prot: TICN3_HUMAN, Q9BQ16
Function: May participate in diverse steps of neurogenesis. Inhibits the processing of pro-matrix
metalloproteinase 2 (MMP-2) by MT1-MMP and MT3-MMP. May interfere with tumor invasion

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the SPOCK3 gene  

Entrez Gene cytogenetic band: 4q32.3   Ensembl cytogenetic band:  4q32.3   HGNC cytogenetic band: 4q32.3

SPOCK3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 4         GeneLoc Exon Structure

GeneLoc location for GC04M167891:     (about GC identifiers)

Start:
167,891,110 bp from pter
End:
168,392,316 bp from pter
Size:
501,207 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000004.10  NT_022792.17  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: TICN3_HUMAN, Q9BQ16 (See protein sequence)
Recommended Name: Testican-3 precursor  
Size: 436 amino acids; 49429 Da
Subcellular location: Secreted, extracellular space, extracellular matrix
Secondary accessions: B2R7M7 O75705 Q6UW53 Q96Q26
Alternative splicing: 3 isoforms:  Q9BQ16-3   Q9BQ16-1   Q9BQ16-2   

Post-translational modifications:

  • Contains chondroitin sulfate and heparan sulfate O-linked oligosaccharides (By similarity)1
  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins (2 alternative transcripts): 
    NP_001035249.1  NP_058646.2  


    ENSEMBL proteins: 
    ENSP00000349677 ENSP00000350153 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (SPOCK3)
    Human Recombinant Proteins from Abnova (SPOCK3)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    2 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005576 extracellular region IEA--
    GO:0005578 proteinaceous extracellular matrix IEA--
    About this table

    Antibodies for SPOCK3: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    R&D Systems Antibodies for SPOCK3 (Testican 3/SPOCK3)
    Antibodies from Abcam (SPOCK3), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (SPOCK3)
    Novus Biologicals Antibodies for SPOCK3

    Assays for SPOCK3: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    5 InterPro domains/families:
     IPR002350 Prot_inh_Kazal
     IPR011992 EF-Hand_type
     IPR019577 SPARC/Testican_Ca-bd-dom
     IPR000716 Thyroglobulin_1
     IPR011497 Prot_Inh_Kazal_2


       GeneDecks  SPOCK3 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9BQ16

    ProtoNet protein and cluster: Q9BQ16

    2 Blocks protein families:
    IPB000716 Thyroglobulin type-1
    IPB011497 Protease inhibitor


    UniProtKB/Swiss-Prot: TICN3_HUMAN, Q9BQ16
    Similarity: Contains 1 Kazal-like domain
    Similarity: Contains 1 thyroglobulin type-1 domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (SPOCK3)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (SPOCK3)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_016950

                  Applied Biosystems Silencer® siRNAs for SPOCK3

                  Sigma-Aldrich siRNA for SPOCK3  
                         Sigma-Aldrich shRNA Panels and shRNA for SPOCK3  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for SPOCK3
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001040159
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001040159
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_001040159 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_016950

    UniProtKB/Swiss-Prot: TICN3_HUMAN, Q9BQ16
    Function: May participate in diverse steps of neurogenesis. Inhibits the processing of pro-matrix
    metalloproteinase 2 (MMP-2) by MT1-MMP and MT3-MMP. May interfere with tumor invasion

    3 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004857 enzyme inhibitor activity IEA--
    GO:0005509 calcium ion binding IEA--
    GO:0008191 metalloendopeptidase inhibitor activity IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section



    2 Interacting proteins for SPOCK3 (ENSP000003496773 Q9BQ162) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    MMP14ENSP000003082083STRING (score=.825)
    --Q9WMX22MINT-7047918
    About this table

    1 Gene Ontology (GO) biological process term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007165 signal transduction IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for SPOCK3

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (SPOCK3)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (SPOCK3)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_016950

                  Sigma-Aldrich siRNA for SPOCK3  
                         Sigma-Aldrich shRNA Panels and shRNA for SPOCK3  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_001040159  NM_016950  

    REFSEQ mRNAs for SPOCK3 gene (2 alternative transcripts): 

    NM_001040159.1   NM_016950.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_001040159  NM_016950  

                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001040159
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001040159
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_001040159 

    Additional cDNA sequence: 

    AB056866.1 AJ001454.1 AK091078.1 AK294293.1 AK294346.1 AK294637.1 AK295015.1 AK295281.1 
    AK295407.1 AK295414.1 AK296291.1 AK313042.1 AY358973.1 BC000460.2 BC003017.1 BC013983.2 
    CR607485.1 

    10 DOTS entries:

    DT.40112755  DT.75113992  DT.100835778  DT.121268883  DT.121268866  DT.97858294  DT.121268802  DT.121268805 
    DT.95079316  DT.95079317 

    24/82 AceView cDNA sequences (see all 82 ):

    AA861657 AA084138 CR607485 N47945 BP361814 BX463580 AW250783 BC000460 
    AL120454 BF222625 BC003017 BI552050 AI038716 BI600087 BI548904 BG705231 
    BI602818 AW023630 BF309481 BI603782 BC013983 BM505167 BP350070 BI597096 

    highest scoring ESTs for SPOCK3:

    AJ001454 AI808090 AB056866 AK091078 AY358973 BC000460 BC003017 BC013983 BE267201 BE267395 

    Unigene Cluster for SPOCK3:

    Sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 3
    Hs.481133  [show with all ESTs]
    Unigene Representative Sequence: NM_016950


    GeneLoc Exon Structure

    2 Ensembl transcripts including schematic representations:
    ENST00000357154  ENST00000357545  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    SPOCK3 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for SPOCK3

    1 / 2 / 3

    9 probe-sets matching SPOCK3 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    33596_at2, 3 U95-A 1 1.00 1.00 0.98 0.75 AJ001454 1.00 0.90 0.95 1
    64676_at2, 3 U95-C 1 1.00 1.00 0.98 1.35 AI808090 0.80 1.00 0.91 1

    235342_at2, 3 U133-B 1 1.00 1.00 -- -- AI808090 0.80 1.00 0.91 1

    206434_at2, 3 U133-A 1 1.00 1.00 -- -- NM_016950 0.60 1.00 0.82 1

    206433_s_at2, 3 U133-A 1 1.00 1.00 -- -- BC000460 0.80 0.88 0.84 1

    235342_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    206434_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    206433_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    1554418_s_at2 U133Plus2 1 0.91 1.00 -- -- -- -- -- -- --
    GeneDecks  SPOCK3 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: --

    SOURCE GeneReport for Unigene cluster: Hs.481133

    Expression variation in blood from EXPOLDB for SPOCK3

    UniProtKB/Swiss-Prot: TICN3_HUMAN, Q9BQ16
    Tissue specificity: Expressed in brain

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for SPOCK3 gene from 5 species
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    chimpanzee
    (Pan troglodytes)
    SPOCK31   -- sparc/osteonectin, cwcv and kazal-like domains proteoglycan more 99.39(n)
    99.31(a)
    461594  XM_001151039.1  XP_001151039.1 
    rat
    (Rattus norvegicus)
    Spock31   -- sparc/osteonectin, cwcv and kazal-like domains proteoglycan more 89.12(n)
    89.2(a)
    306404  XM_224788.4  XP_224788.3 
    mouse
    (Mus musculus)
    Spock31, 5 85
    sparc/osteonectin, cwcv and kazal-like domains proteoglycan more1, 5 89.53(n)1
    90.14(a)1
    729021  NM_023689.21  NP_076178.11 
     AI4284715  AJ2789985  (see all 12)
    chicken
    (Gallus gallus)
    SPOCK31   -- sparc/osteonectin, cwcv and kazal-like domains proteoglycan more 83.56(n)
    87.5(a)
    422425  XM_420393.2  XP_420393.2 
    zebrafish
    (Danio rerio)
    Dr.129412   -- Transcribed sequence with weak similarity to protein more 75.16(n)  CK357112.1 
    About this table        Species with no ortholog for SPOCK3

    ENSEMBL Gene Tree for SPOCK3
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for SPOCK3 gene
    SPOCK12  SPOCK22  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/2826 NCBI SNPs in SPOCK3 are shown (see all 2826 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 845)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 4 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs76741601,2
    A,C,F,H167892177(+) ACTCAA/TCTAGG 2 -- ut3111Minor allele frequency- T:0.36NA EU EA WA 980
    rs37622441,2
    C,F,H167892057(-) TCTAAT/CAGGAC 2 -- ut31 ese320Minor allele frequency- C:0.33EA NA EU WA 3248
    rs170531211,2
    C,F,H168394169(+) TTAGCT/CACAAA 2 -- ng317Minor allele frequency- C:0.04NA EU EA WA 560
    rs68469301,2
    A,C,F167891535(+) AGAAAC/GAAACG 2 -- ut314Minor allele frequency- G:0.35EA NA 216
    rs20541471,2
    A,C,F167892299(-) ATTTTC/TTGAAA 2 -- ut31 ese33Minor allele frequency- T:0.36NA 140
    rs76952941,2
    C,F,H167892378(+) AAAAAC/TTTATT 2 -- ut319Minor allele frequency- T:0.36NA EU EA WA 738
    rs96856451,2
    C,F,H168158100(+) ACTAAT/CGCAGA 2 V/I mis1 ese311Minor allele frequency- C:0.05EU EA WA NA 976
    rs176005101,2
    C,F,H168393028(+) TGGCAG/ACTTTA 2 -- ng3112Minor allele frequency- A:0.23NA EU EA WA 998
    --
    rs100213431,2
    A,C167890972(+) AAGAGC/GGCTAA 2 -- ng510--------
    rs37622451,2
    C,F167891810(-) AAAGTC/TTGTCA 2 -- ut314Minor allele frequency- N:0.01EA NA 216
    About this table

    HapMap Linkage Disequilibrium images for SPOCK3 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 607989

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    7 PubMed articles for SPOCK3 gene:
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 50859 HGNC: 13565 AceView: SPOCK3 Ensembl:ENSG00000196104 euGenes: HUgn50859
    ECgene: SPOCK3 H-InvDB: SPOCK3
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    ATLAS Chromosomes in Cancer entry for SPOCK3 Genetics and Cytogenetics in Oncology and Haematology
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for SPOCK3:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
      Invitrogen Clones  Invitrogen Q-PCR Products
      Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for SPOCK3-related products

     Millipore Custom Antibody & Bulk Services
     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for SPOCK3 Browse antibodies at Sigma-Aldrich
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for SPOCK3 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Recombinant & Natural Proteins  

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     Custom Service for Rabbit Pab from Full-length Protein
      
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     Search OriGene for SPOCK3
     Search Tocris compounds for SPOCK3




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for SPOCK3

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