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SPOCK1 Gene

protein-coding   GIFtS: 56

GC05M136339
sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 1
(Previous names: sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbols: TIC1, SPOCK)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
FLJ37170 2
OTTHUMP00000159433 2
SPOCK 2, 3
TESTICAN 2
TIC1 2, 3
TICN1 3
testican-1 1, 2
Descriptions
Protein SPOCK 3
sparc/osteonectin, cwcv and kazal-like domains proteoglycan
(testican) 1
sparc/osteonectin, cwcv and kazal-like domains proteoglycan
(testican) 1 2
sparc/osteonectin, cwcv and kazal-like domains proteoglycan
1 2
External Ids
HGNC: 112511
Entrez Gene: 66952
UniProtKB: Q086293
Ensembl: ENSG000001523777
Search outside databases for aliases for SPOCK1 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for SPOCK1:
This gene encodes the protein core of a seminal plasma proteoglycan containing chondroitin- and
heparan-sulfate chains. The protein's function is unknown, although similarity to
thyropin-type cysteine protease-inhibitors suggests its function may be related to protease
inhibition. [provided by RefSeq]

UniProtKB/Swiss-Prot: TICN1_HUMAN, Q08629
Function: May play a role in cell-cell and cell-matrix interactions. May contribute to various
neuronal mechanisms in the central nervous system

Gene Wiki entry for SPOCK1

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the SPOCK1 gene  

Entrez Gene cytogenetic band: 5q31   Ensembl cytogenetic band:  5q31.2   HGNC cytogenetic band: 5q31.2

SPOCK1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 5         GeneLoc Exon Structure

GeneLoc location for GC05M136339:     (about GC identifiers)

Start:
136,338,886 bp from pter
End:
136,862,936 bp from pter
Size:
524,051 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000005.8  NT_034772.5  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: TICN1_HUMAN, Q08629 (See protein sequence)
Recommended Name: Testican-1 precursor  
Size: 439 amino acids; 49124 Da
Subcellular location: Secreted, extracellular space, extracellular matrix
Secondary accessions: B3KSW3 Q59EW0 Q8N630 Q9UCL8

Post-translational modifications:

  • Contains chondroitin sulfate and heparan sulfate O-linked oligosaccharides1
  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_004589.1  

    ENSEMBL proteins: 
    ENSP00000282223 ENSP00000378401 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    R&D Systems Recombinant & Natural Proteins for SPOCK1 (Testican 1/SPOCK1)
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (SPOCK1)
    Human Recombinant Proteins from Abnova (SPOCK1)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    2 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005576 extracellular region IEA--
    GO:0005578 proteinaceous extracellular matrix IEA--
    About this table

    Antibodies for SPOCK1: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    R&D Systems Antibodies for SPOCK1 (Testican 1/SPOCK1)
    Antibodies from Abcam (SPOCK1), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (SPOCK1)
    Novus Biologicals Antibodies for SPOCK1

    Assays for SPOCK1: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    5 InterPro domains/families:
     IPR002350 Prot_inh_Kazal
     IPR011992 EF-Hand_type
     IPR019577 SPARC/Testican_Ca-bd-dom
     IPR000716 Thyroglobulin_1
     IPR011497 Prot_Inh_Kazal_2


       GeneDecks  SPOCK1 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q08629

    ProtoNet protein and cluster: Q08629

    2 Blocks protein families:
    IPB000716 Thyroglobulin type-1
    IPB011497 Protease inhibitor


    UniProtKB/Swiss-Prot: TICN1_HUMAN, Q08629
    Similarity: Contains 1 Kazal-like domain
    Similarity: Contains 1 thyroglobulin type-1 domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (SPOCK1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (SPOCK1)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_004598

                  Applied Biosystems Silencer® siRNAs for SPOCK1

                  Sigma-Aldrich siRNA for SPOCK1  
                         Sigma-Aldrich shRNA Panels and shRNA for SPOCK1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for SPOCK1
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_004598
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_004598
                                     untagged cDNA clone in CMV expression vector: NM_004598 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_004598

    UniProtKB/Swiss-Prot: TICN1_HUMAN, Q08629
    Function: May play a role in cell-cell and cell-matrix interactions. May contribute to various
    neuronal mechanisms in the central nervous system

    1 MGI mutant phenotype (inferred from 1 allele(MGI details for Spock1):

    normal

    1 Gene Ontology (GO) molecular function term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005509 calcium ion binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section



    3 Interacting proteins for SPOCK1 (ENSP000002822233) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    MMP14ENSP000003082083STRING (score=.922)
    MMP2ENSP000002190703STRING (score=.858)
    MMP16ENSP000002866143STRING (score=.85)
    About this table

    5/6 Gene Ontology (GO) biological process terms (links to tree view) (see all 6 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006928 cell motion TAS8389704
    GO:0007155 cell adhesion TAS8389704
    GO:0007165 signal transduction IEA--
    GO:0007275 multicellular organismal development TAS8389704
    GO:0007399 nervous system development TAS9181134
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for SPOCK1

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (SPOCK1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (SPOCK1)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_004598

                  Sigma-Aldrich siRNA for SPOCK1  
                         Sigma-Aldrich shRNA Panels and shRNA for SPOCK1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_004598  

    REFSEQ mRNAs for SPOCK1 gene: 

    NM_004598.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_004598  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_004598
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_004598
                                     untagged cDNA clone in CMV expression vector: NM_004598 

    Additional cDNA sequence: 

    AB209701.1 AF231124.1 AK094489.1 BC030691.2 CR606032.1 

    12 DOTS entries:

    DT.97857476  DT.95124677  DT.101967721  DT.95331642  DT.120815763  DT.100037804  DT.120815755  DT.100711206 
    DT.120815632  DT.100727843  DT.120815883  DT.91760126 

    1 AceView cDNA sequence:

    BI550122 

    highest scoring ESTs for SPOCK1:

    AF231124 AI674088 BC030691 X73608 AA027807 AA027861 AA035654 AA040054 AA045197 AA045381 

    Unigene Cluster for SPOCK1:

    Sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 1
    Hs.643338  [show with all ESTs]
    Unigene Representative Sequence: NM_004598


    GeneLoc Exon Structure

    2 Ensembl transcripts including schematic representations:
    ENST00000282223  ENST00000394945  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    SPOCK1 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for SPOCK1

    1 / 2 / 3

    4 probe-sets matching SPOCK1 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    78788_at2, 3 U95-E 1 1.00 1.00 0.99 1.27 AI674088 0.80 1.00 0.91 1
    39100_at2, 3 U95-A 1 0.88 1.00 0.99 0.73 X73608 0.80 1.00 0.91 1

    202363_at2, 3 U133-A 1 1.00 1.00 -- -- AF231124 0.80 1.00 0.91 1

    202363_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  SPOCK1 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: AGAATACCTT

    SOURCE GeneReport for Unigene cluster: Hs.643338
    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for SPOCK1 gene from 5/7 species (see all 7 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    SPOCK11   -- sparc/osteonectin, cwcv and kazal-like domains proteoglycan more 91.8(n)
    95.86(a)
    474688  XM_531914.2  XP_531914.2 
    chimpanzee
    (Pan troglodytes)
    SPOCK11   -- sparc/osteonectin, cwcv and kazal-like domains proteoglycan more 99.39(n)
    98.86(a)
    462085  XM_517947.2  XP_517947.2 
    cow
    (Bos taurus)
    SPOCK11   -- sparc/osteonectin, cwcv and kazal-like domains proteoglycan more 91.34(n)
    94.31(a)
    511211  NM_001075500.1  NP_001068968.1 
    rat
    (Rattus norvegicus)
    Spock11   -- sparc/osteonectin, cwcv and kazal-like domains proteoglycan more 83.37(n)
    87.02(a)
    306759  XM_225160.4  XP_225160.4 
    mouse
    (Mus musculus)
    Spock11, 5 135
    sparc/osteonectin, cwcv and kazal-like domains proteoglycan more1, 5 89.75(n)1
    95.22(a)1
    207451  NM_009262.21  NP_033288.21 
     AI3233135  AK0483585  (see all 11)
    About this table        Species with no ortholog for SPOCK1

    ENSEMBL Gene Tree for SPOCK1
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for SPOCK1 gene
    SPOCK32  SPOCK22  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/2689 NCBI SNPs in SPOCK1 are shown (see all 2689 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 1092)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 5 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs24290851,2
    A,C,F,H136340686(-) TTCATC/TTATCA 1 -- ut317Minor allele frequency- T:0.41EU EA WA NA 554
    rs65963601,2
    A,C,F,H136339289(+) CATTTG/CCTGCT 1 -- ut317Minor allele frequency- C:0.01EU EA WA NA 546
    rs31922431,2
    A,C,F,H136340950(-) AACCCT/CGGGTT 1 -- ut318Minor allele frequency- C:0.38MN NA EU EA WA 742
    rs100640121,2
    A,C,F,H136864468(+) atgcaT/Cggcaa 1 -- ng314Minor allele frequency- C:0.44EU EA WA 406
    rs65963611,2
    A,C,F,H136339669(+) AGGACT/CATTTC 1 -- ut319Minor allele frequency- C:0.25NA MN EA EU WA 1810
    rs100432151,2
    A,C,F,H136339938(+) TGTCTA/GTTGAG 1 -- ut319Minor allele frequency- C:0.02NA MN EA EU WA 810
    rs10518541,2
    C,F,H,O136341912(+) AGGGTG/TGGGGC 1 -- ut315Minor allele frequency- T:0.32MN EU EA WA 602
    rs77355731,2
    C,F,H136339441(+) CTGGGA/TTTTAA 1 -- ut3111Minor allele frequency- T:0.04EU EA WA NA 982
    rs10518531,2
    C,F,H136341700(+) AGATAT/GAATGA 1 -- ut3115Minor allele frequency- G:0.18NA EU EA WA 1400
    rs177027451,2
    F,H136341278(+) CGTGTC/TGTGTG 1 -- ut317Minor allele frequency- T:0.02NA EU EA WA 554
    About this table

    HapMap Linkage Disequilibrium images for SPOCK1 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 602264

    Human Genome Epidemiology Navigator: SPOCK1 (1 document)

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/18 PubMed articles for SPOCK1 gene (see all 18 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 6695 HGNC: 11251 AceView: SPOCK.2 Ensembl:ENSG00000152377 euGenes: HUgn6695
    ECgene: SPOCK1 H-InvDB: SPOCK1
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    ATLAS Chromosomes in Cancer entry for SPOCK1 Genetics and Cytogenetics in Oncology and Haematology
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for SPOCK1:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
      Invitrogen Clones  Invitrogen Q-PCR Products
      Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for SPOCK1-related products

     Millipore Custom Antibody & Bulk Services
     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for SPOCK1 Browse antibodies at Sigma-Aldrich
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for SPOCK1 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
     Antibodies   Primer Pairs  
     Cell Culture Products   ELISAs  
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     Recombinant & Natural Proteins  

     Recombinant Proteins (SPOCK1)
     Antibodies (SPOCK1)
     Chimera RNAi (SPOCK1)
     Custom Service for Mouse Mab
     Custom Service for Rabbit Pab from Full-length Protein
      
     Search for Antibodies & Assays

     Recombinant Proteins
    (SPOCK1)
     Antibodies (SPOCK1)
     Search OriGene for SPOCK1
     Search Tocris compounds for SPOCK1




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for SPOCK1

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