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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

SPIC Gene

protein-coding   GIFtS: 45
GCID: GC12P101870

Spi-C transcription factor (Spi-1/PU.1 related)

 Explore 2 diseases affiliated with
SPIC via our new
 Human Malady Compendium 
Biological research products
for SPIC
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Spi-C Transcription Factor (Spi-1/PU.1 Related)1 2
SPI-C1 2
MGC406111
Transcription Factor Spi-C2

External Ids:    HGNC: 295491   Entrez Gene: 1215992   Ensembl: ENSG000001662117   OMIM: 6125685   UniProtKB: Q8N5J43   

Export aliases for SPIC gene to outside databases

Previous GC identifers: GC12P101853 GC12P100371 GC12P100393 GC12P098930


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: SPIC_HUMAN, Q8N5J4
Function: Controls the development of red pulp macrophages required for red blood cells recycling and iron homeostasis.
Transcription factor that binds to the PU-box, a purine-rich DNA sequence (5'-GAGGA[AT]-3') that can act as a
lymphoid-specific enhancer. Regulates VCAM1 gene expression (By similarity)




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000012.11  NC_018923.1  NT_029419.12  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the SPIC gene promoter:
         STAT5B   Elk-1   MEF-2   CREB   MEF-2A   POU2F1   deltaCREB   POU2F1a   aMEF-2   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidSPIC promoter sequence
   Search SABiosciences Chromatin IP Primers for SPIC

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat SPIC


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 12q23.2   Ensembl cytogenetic band:  12q23.2   HGNC cytogenetic band: 12q23

SPIC Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
SPIC gene location

GeneLoc information about chromosome 12         GeneLoc Exon Structure

GeneLoc location for GC12P101870:  view genomic region     (about GC identifiers)

Start:
101,869,199 bp from pter      End:
101,880,775 bp from pter
Size:
11,577 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: SPIC_HUMAN, Q8N5J4 (See protein sequence)
Recommended Name: Transcription factor Spi-C  
Size: 248 amino acids; 29180 Da
Subunit: Binds DNA as a monomer
Subcellular location: Nucleus (Probable)

Explore the universe of human proteins at neXtProt for SPIC: NX_Q8N5J4

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q8N5J4

  • SPIC Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_689536.1  
    ENSEMBL proteins: 
     ENSP00000448580   ENSP00000299272  

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    Uscn Proteins for SPIC

    Gene Ontology (GO): 1 cellular component term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IEA--


    SPIC for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    SPIC for domains           About GeneDecksing

    2 InterPro domains/families:
     IPR011991 WHTH_trsnscrt_rep_DNA-bd_dom
     IPR000418 Ets_dom

    Graphical View of Domain Structure for InterPro Entry Q8N5J4

    ProtoNet protein and cluster: Q8N5J4

    UniProtKB/Swiss-Prot: SPIC_HUMAN, Q8N5J4
    Similarity: Belongs to the ETS family
    Similarity: Contains 1 ETS DNA-binding domain


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: SPIC_HUMAN, Q8N5J4
    Function: Controls the development of red pulp macrophages required for red blood cells recycling and iron homeostasis.
    Transcription factor that binds to the PU-box, a purine-rich DNA sequence (5'-GAGGA[AT]-3') that can act as a
    lymphoid-specific enhancer. Regulates VCAM1 gene expression (By similarity)

    miRNA
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    Inhib. RNA
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    Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003700sequence-specific DNA binding transcription factor activity IEA--
    GO:0043565sequence-specific DNA binding IEA--


    SPIC for ontologies           About GeneDecksing


    1 GenomeRNAi human phenotype for SPIC:
     Increased S DNA content, incre 

    Animal Models:
         Mouse knock-out Spictm1.1Kmm for SPIC
         4 MGI mutant phenotypes (inferred from 2 alleles(MGI details for Spic):
     hematopoietic system  homeostasis/metabolism  immune system  mortality/aging 

    SPIC for phenotypes           About GeneDecksing


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for SPIC

    Gene Ontology (GO): 3 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001824blastocyst development IEA--
    GO:0006351transcription, DNA-dependent IEA--
    GO:0045944positive regulation of transcription from RNA polymerase II promoter IEA--


    SPIC for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for SPIC
    Search CenterWatch for drugs/clinical trials and news about SPIC 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
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    About This Section

    REFSEQ mRNAs for SPIC gene: 
    NM_152323.1  

    Unigene Cluster for SPIC:

    Spi-C transcription factor (Spi-1/PU.1 related)
    Hs.577097  [show with all ESTs]
    Unigene Representative Sequence: AF518404
    2 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000551346(uc001tid.3 uc010svp.2 uc009zua.3 uc021rcq.1)
    ENST00000299272

    miRNA
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    Inhib. RNA
    Products:
         
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    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for SPIC (see all 7)
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    Clone
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    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat SPIC 
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      QIAGEN QuantiFast Probe-based Assays in human, mouse, rat SPIC

    Additional cDNA sequence: 

    AF518404.1 BC032317.1 

    1 DOTS entry:

    DT.100015091 

    7 AceView cDNA sequences:

    BC032317 NM_152323 BG567207 BI770622 BI838200 AF518404 R10669 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    SPIC expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: ATTGCTAAAT

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image

    SPIC expression in embryonic tissues and stem cells
    Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table
    3 LifeMap In Vivo Development Anatomical Compartments/Cells 
    Tissue Anatomical Compartment CellCategory (developmental path)
    HeartMyocardiumMature Cardiac FibroblastsEpicardium
    BoneMaxillary ProcessBone
    LiverLiver LobuleLiver
    Expression: Positive    Negative     Selective marker
    Experimental details: Curated     Microarrays     In-situ hybridization
    Stem Cell Differentiation: 1 LifeMap Cell 
    NameCategory
    Blimp1- mVenus and stella-ECFP genetically modified stem cells (Embryonic Stem Cell)Early Embryo, Inner Cell Mass

    See SPIC Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for SPIC

    SOURCE GeneReport for Unigene cluster: Hs.577097

    UniProtKB/Swiss-Prot: SPIC_HUMAN, Q8N5J4
    Tissue specificity: Preferentially detected in fetal and adult spleen, lymph nodes and at lower levels in bone marrow
    and fetal liver

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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for SPIC

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of chordates.

    Orthologs for SPIC gene from 3/11 species (see all 11)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    chicken
    (Gallus gallus)
    Aves SPIC1 Spi-C transcription factor (Spi-1/PU.1 related) 69.86(n)
    65.83(a)
      427915  XM_425488.2  XP_425488.2 
    lizard
    (Anolis carolinensis)
    Reptilia SPIC6
    --
    56(a)
    1 ↔ 1
    5(17190674-17196200)
    zebrafish
    (Danio rerio)
    Actinopterygii spic1 Spi-C transcription factor (Spi-1/PU.1 related) 52.43(n)
    50(a)
      447882  NM_001004621.1  NP_001004621.1 


    ENSEMBL Gene Tree for SPIC (if available)
    TreeFam Gene Tree for SPIC (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for SPIC gene
    SPIB2  SPI12  
    3 SIMAP similar genes for SPIC using alignment to 1 protein entry:     SPIC_HUMAN:
    SPI1    ELF5    SPIB

    SPIC for paralogs           About GeneDecksing


    5 Pseudogenes.org Pseudogenes for SPIC
    PGOHUM00000242206 PGOHUM00000236914 PGOHUM00000237363 PGOHUM00000245808 PGOHUM00000235494


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/247 NCBI SNPs in SPIC are shown (see all 247    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 12 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs793215671,2
    F,--101868727(+) AGACCG/AATTTG 1 -- us2k12Minor allele frequency- A:0.05WA EA 238
    rs1823422171,2
    --101868795(+) GAATTC/GGGAGA 1 -- us2k10--------
    rs73094471,2
    C,F,A,H,--101868843(+) GTTGCA/CTGGGT 1 -- us2k119Minor allele frequency- C:0.38NA NS EA WA CSA 906
    rs352640291,2
    C,F,--101868895(+) CCTAAG/ATNNNN 1 -- us2k19Minor allele frequency- A:0.34NA WA CSA 252
    rs1149226261,2
    F,--101868899(+) AGTTGG/AGCGGG 1 -- us2k11Minor allele frequency- A:0.09WA 118
    rs1866952461,2
    --101869075(+) TCCTTA/CAAAAC 1 -- us2k10--------
    rs73101631,2
    C,F,H,--101869220(+) CTACCG/ATTGAA 1 -- us2k1 ese34Minor allele frequency- A:0.01NS EA 420
    rs1157252261,2
    F,--101869279(+) CTTCCC/TGGTAA 1 -- us2k11Minor allele frequency- T:0.03WA 118
    rs1392757041,2
    --101869324(+) TCACTA/GTTCAT 1 -- us2k10--------
    rs1899302791,2
    --101869452(+) CTTGTA/CATAGC 1 -- us2k10--------

    HapMap Linkage Disequilibrium report for SPIC (101869199 - 101880775 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for SPIC: --

    SABiosciences Cancer Mutation PCR Assays
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    SPIC for disorders           About GeneDecksing

    OMIM gene information: 612568    OMIM disorders: --

    2 diseases for SPIC:    About MalaCards
    wolman disease    lipomatosis

    3 diseases from the University of Copenhagen DISEASES database for SPIC:
    Wolman disease     Dilated cardiomyopathy     Lipomatosis

    Export disorders for SPIC gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for SPIC gene integrated from 9 sources:
    (articles sorted by number of sources associating them with SPIC)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Genomic structure of mouse SPI-C and genomic structure and expression pattern of human SPI-C. (PubMed id 12459275)1, 2, 3 Carlsson R.... Leanderson T. (2002)
    2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    3. Interactions of pathological hallmark proteins: tubul in polymerization promoting protein/p25, beta-amyloid, and alpha-synuclein. (PubMed id 21832049)1 OlA!h J....OvA!di J. (2011)
    4. SPI-C and STAT6 can cooperate to stimulate IgE germline transcription. (PubMed id 16647686)1 Carlsson R....Leanderson T. (2006)
    5. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 121599 HGNC: 29549 AceView: SPIC Ensembl:ENSG00000166211 euGenes: HUgn121599
    ECgene: SPIC H-InvDB: SPIC

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for SPIC Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for SPIC gene:
    Search GeneIP for patents involving SPIC

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences),
    In Situ Hybridization Assays from
    Advanced Cell Diagnostics
    About This Section

     
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     QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat SPIC
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     GenScript Custom Purified and Recombinant Proteins Services for SPIC GenScript cDNA clones with any tag delivered in your preferred vector for SPIC
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     Regulatory tfbs in SPIC promoter
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     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies
     Novus Tissue Slides
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     SPIC Proteins, Antibodies, CLIAs, and ELISAs
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     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat SPIC
           
    GeneCards Homepage - Last full update: 19 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 26 Apr 2013

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    Category
    VWF
    (GIFTS: 73)
    von Willebrand factor
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 6 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 


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