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Aliases for SPI1 Gene

Aliases for SPI1 Gene

  • Spi-1 Proto-Oncogene 2 3
  • Hematopoietic Transcription Factor PU.1 2 3
  • 31 KDa Transforming Protein 2 3
  • 31 KDa-Transforming Protein 3 4
  • SFPI1 3 6
  • Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 3
  • Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene 2
  • Transcription Factor PU.1 3
  • SPI-1 3
  • SPI-A 3
  • PU.1 3
  • OF 3

External Ids for SPI1 Gene

Summaries for SPI1 Gene

Entrez Gene Summary for SPI1 Gene

  • This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

GeneCards Summary for SPI1 Gene

SPI1 (Spi-1 Proto-Oncogene) is a Protein Coding gene. Diseases associated with SPI1 include primary effusion lymphoma. Among its related pathways are Pathways in cancer and Epstein-Barr virus infection. GO annotations related to this gene include sequence-specific DNA binding transcription factor activity and RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity. An important paralog of this gene is SPIC.

UniProtKB/Swiss-Prot for SPI1 Gene

  • Binds to the PU-box, a purine-rich DNA sequence (5-GAGGAA-3) that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity).

Gene Wiki entry for SPI1 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for SPI1 Gene

Genomics for SPI1 Gene

Genomic Location for SPI1 Gene

Start:
47,354,858 bp from pter
End:
47,378,576 bp from pter
Size:
23,719 bases
Orientation:
Minus strand

Genomic View for SPI1 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for SPI1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for SPI1 Gene

Regulatory Elements for SPI1 Gene

Transcription factor binding sites by QIAGEN in the SPI1 gene promoter:

Proteins for SPI1 Gene

  • Protein details for SPI1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P17947-SPI1_HUMAN
    Recommended name:
    Transcription factor PU.1
    Protein Accession:
    P17947
    Secondary Accessions:

    Protein attributes for SPI1 Gene

    Size:
    270 amino acids
    Molecular mass:
    31083 Da
    Quaternary structure:
    • Binds DNA as a monomer. Interacts with CEBPD and NONO (By similarity). Interacts with RUNX1 and SPIB. Interacts with GFI1; the interaction represses SPI1 transcriptional activity.
    SequenceCaution:
    • Sequence=CAA36281.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};

    Alternative splice isoforms for SPI1 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for SPI1 Gene

Proteomics data for SPI1 Gene at MOPED

Post-translational modifications for SPI1 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for SPI1 Gene

No data available for DME Specific Peptides for SPI1 Gene

Domains for SPI1 Gene

Protein Domains for SPI1 Gene

InterPro:
ProtoNet:

UniProtKB/Swiss-Prot:

SPI1_HUMAN
Domain:
  • Contains 1 ETS DNA-binding domain.:
    • P17947
Family:
  • Belongs to the ETS family.:
    • P17947
genes like me logo Genes that share domains with SPI1: view

No data available for Gene Families for SPI1 Gene

Function for SPI1 Gene

Molecular function for SPI1 Gene

GENATLAS Biochemistry: spleen focus forming virus (SFFV) proviral integration oncogene spi1,Pu-1 homolog,expressed in various hemapoietic cell lines,required for the development of both myeloid and lymphoid lineages
UniProtKB/Swiss-Prot Function: Binds to the PU-box, a purine-rich DNA sequence (5-GAGGAA-3) that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity).
UniProtKB/Swiss-Prot Induction: Highly expressed in both FV-P and FV-A-induced erythro-leukemia cell lines that have undergone rearrangements of the SPI1 gene due to the insertion of SFFV. Negatively regulated by microRNA-155 (miR-155).

Gene Ontology (GO) - Molecular Function for SPI1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000978 RNA polymerase II core promoter proximal region sequence-specific DNA binding IEA --
GO:0000979 RNA polymerase II core promoter sequence-specific DNA binding IEA --
GO:0000980 RNA polymerase II distal enhancer sequence-specific DNA binding ISS --
GO:0000981 sequence-specific DNA binding RNA polymerase II transcription factor activity IBA --
GO:0001047 core promoter binding IDA 20139074
genes like me logo Genes that share ontologies with SPI1: view
genes like me logo Genes that share phenotypes with SPI1: view

Animal Models for SPI1 Gene

MGI Knock Outs for SPI1:
Targeting motifs for SPI1 Gene
HOMER Transcription Factor Regulatory Elements motif SPI1
  • Consensus sequence: AGAGGAAGTG Submotif: canonical Cell Type: ThioMac GeoId: GSE21512

No data available for Enzyme Numbers (IUBMB) and Transcription Factor Targeting for SPI1 Gene

Localization for SPI1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for SPI1 Gene

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for SPI1 Gene COMPARTMENTS Subcellular localization image for SPI1 gene
Compartment Confidence
nucleus 5

Gene Ontology (GO) - Cellular Components for SPI1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000790 nuclear chromatin IDA 15304486
GO:0005634 nucleus --
genes like me logo Genes that share ontologies with SPI1: view

Pathways for SPI1 Gene

genes like me logo Genes that share pathways with SPI1: view

Gene Ontology (GO) - Biological Process for SPI1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000122 negative regulation of transcription from RNA polymerase II promoter IEA --
GO:0001944 vasculature development --
GO:0002320 lymphoid progenitor cell differentiation IEA --
GO:0002573 myeloid leukocyte differentiation --
GO:0006355 regulation of transcription, DNA-templated --
genes like me logo Genes that share ontologies with SPI1: view

Compounds for SPI1 Gene

(6) Novoseek inferred chemical compound relationships for SPI1 Gene

Compound -log(P) Hits PubMed IDs
5-aza-2deoxycytidine 21.6 1
tyrosine 20.7 4
leucine 10.7 1
serine 5.61 3
proline 0 1
genes like me logo Genes that share compounds with SPI1: view

Transcripts for SPI1 Gene

Unigene Clusters for SPI1 Gene

Spleen focus forming virus (SFFV) proviral integration oncogene spi1:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for SPI1 Gene

ExUns: 1 ^ 2 ^ 3a · 3b ^ 4 ^ 5a · 5b ^ 6 ^ 7a · 7b
SP1:
SP2:
SP3: - -

Relevant External Links for SPI1 Gene

GeneLoc Exon Structure for
SPI1
ECgene alternative splicing isoforms for
SPI1

Expression for SPI1 Gene

mRNA expression in normal human tissues for SPI1 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for SPI1 Gene

This gene is overexpressed in Whole Blood (39.7).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, and MOPED for SPI1 Gene

SOURCE GeneReport for Unigene cluster for SPI1 Gene Hs.502511

genes like me logo Genes that share expressions with SPI1: view

Orthologs for SPI1 Gene

This gene was present in the common ancestor of chordates.

Orthologs for SPI1 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia SPI1 37
  • 71 (a)
OneToOne
cow
(Bos Taurus)
Mammalia SPI1 36
  • 92.96 (n)
  • 95.93 (a)
SPI1 37
  • 95 (a)
OneToOne
dog
(Canis familiaris)
Mammalia SPI1 36
  • 91.73 (n)
  • 96.67 (a)
SPI1 37
  • 73 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Sfpi1 16
Spi1 36
  • 88.02 (n)
  • 88.52 (a)
Spi1 37
  • 88 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia SPI1 37
  • 82 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia SPI1 37
  • 77 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Spi1 36
  • 88.27 (n)
  • 89.26 (a)
chicken
(Gallus gallus)
Aves SPI1 36
  • 77.73 (n)
  • 83.2 (a)
SPI1 37
  • 84 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia SPI1 37
  • 74 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia spi1 36
  • 68.93 (n)
  • 73.6 (a)
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.10886 36
zebrafish
(Danio rerio)
Actinopterygii spi1 36
spi1b 36
  • 65.49 (n)
  • 64.16 (a)
spi1b 37
  • 49 (a)
OneToOne
sea squirt
(Ciona savignyi)
Ascidiacea -- 37
  • 20 (a)
OneToMany
Species with no ortholog for SPI1:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for SPI1 Gene

ENSEMBL:
Gene Tree for SPI1 (if available)
TreeFam:
Gene Tree for SPI1 (if available)

Paralogs for SPI1 Gene

Paralogs for SPI1 Gene

Selected SIMAP similar genes for SPI1 Gene using alignment to 3 proteins:

genes like me logo Genes that share paralogs with SPI1: view

Variants for SPI1 Gene

Sequence variations from dbSNP and Humsavar for SPI1 Gene

SNP ID Clin Chr 11 pos Sequence Context AA Info Type MAF
rs10747 -- 47,354,993(+) TGCCC(C/G)GGTGG utr-variant-3-prime
rs896816 -- 47,372,787(-) GGCAT(A/G)TAATC intron-variant
rs896817 -- 47,372,754(-) AGGAC(A/G)AGGCC intron-variant
rs935914 -- 47,366,048(+) TTACC(C/T)ATCAC intron-variant
rs1057221 -- 47,355,099(-) CTCGC(C/G)TCACC utr-variant-3-prime

Structural Variations from Database of Genomic Variants (DGV) for SPI1 Gene

Variant ID Type Subtype PubMed ID
nsv897323 CNV Loss 21882294
nsv832141 CNV Loss 17160897
nsv509404 CNV Insertion 20534489
nsv825871 CNV Gain 20364138
esv992404 CNV Insertion 20482838
nsv513315 CNV Insertion 21212237
esv275512 CNV Loss 21479260

Relevant External Links for SPI1 Gene

HapMap Linkage Disequilibrium report
SPI1
Human Gene Mutation Database (HGMD)
SPI1

Disorders for SPI1 Gene

(1) University of Copenhagen DISEASES for SPI1 Gene

(14) Novoseek inferred disease relationships for SPI1 Gene

Disease -log(P) Hits PubMed IDs
erythroleukemia 72.5 12
leukemogenesis 40.6 2
microphthalmia 40.2 3
leukemia 38.7 6
hodgkin disease 28.3 7

Relevant External Links for SPI1

Genetic Association Database (GAD)
SPI1
Human Genome Epidemiology (HuGE) Navigator
SPI1
Tumor Gene Database (TGDB):
SPI1
genes like me logo Genes that share disorders with SPI1: view

No data available for UniProtKB/Swiss-Prot for SPI1 Gene

Publications for SPI1 Gene

  1. The human homologue of the putative proto-oncogene Spi-1: characterization and expression in tumors. (PMID: 1693183) Ray D. … Moreau-Gachelin F. (Oncogene 1990) 2 3 4 23
  2. Differential phosphorylations of Spi-B and Spi-1 transcription factors. (PMID: 8632909) Mao C. … Moreau-Gachelin F. (Oncogene 1996) 3 23
  3. The activation domain of transcription factor PU.1 binds the retinoblastoma (RB) protein and the transcription factor TFIID in vitro: RB shows sequence similarity to TFIID and TFIIB. (PMID: 8434021) Hagemeier C. … Kouzarides T. (Proc. Natl. Acad. Sci. U.S.A. 1993) 3 23
  4. The macrophage and B cell-specific transcription factor PU.1 is related to the ets oncogene. (PMID: 2180582) Klemsz M.J. … Maki R.A. (Cell 1990) 3 23
  5. Localization of the human oncogene SPI1 on chromosome 11, region p11.22. (PMID: 2338340) Nguyen V.C. … Moreau-Gachelin F. (Hum. Genet. 1990) 3 23

Products for SPI1 Gene

Sources for SPI1 Gene

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