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Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

SPI1 Gene

protein-coding GIFtS: 66
GCID: GC11M048075

spleen focus forming virus (SFFV) proviral integration oncogene...

Explore 27 diseases affiliated with
SPI1 via

MalaCards

our new
Human Malady Compendium

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1¹ ²		31 KDa-Transforming Protein² ³
SFPI1¹ ² ⁵		31 KDa Transforming Protein²
OF¹ ²		Hematopoietic Transcription Factor PU.1²
PU.1¹ ²		SPI-1 Proto-Oncogene²
SPI-1¹ ²		Transcription Factor PU.1²
SPI-A¹ ²

External Ids:	HGNC: 11241¹	Entrez Gene: 6688²	Ensembl: ENSG00000066336⁷	OMIM: 165170⁵	UniProtKB: P17947³

Export aliases for SPI1 gene to outside databases

Previous GC identifers: GC11M049316 GC11M048254 GC11M047408 GC11M047340 GC11M047332 GC11M047075 GC11M047430

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for SPI1:

This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell

development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of

target genes, and regulates their expression in coordination with other transcription factors and cofactors. The

protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different

isoforms have been found for this gene. (provided by RefSeq, Jul 2008)

UniProtKB/Swiss-Prot: SPI1_HUMAN, P17947

Function: Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer.

This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of

macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity)

Gene Wiki entry for SPI1

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000011.9 NC_018922.1 NT_009237.18

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the SPI1 gene promoter:
AML1a
Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: SPI1 promoter sequence

Search SABiosciences Chromatin IP Primers for SPI1

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat SPI1

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 11p11.2 Ensembl cytogenetic band: 11p11.2 HGNC cytogenetic band: 11p12-p11.22

SPI1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
SPI1 gene location

GeneLoc information about chromosome 11 GeneLoc Exon Structure

GeneLoc location for GC11M048075: view genomic region (about GC identifiers)

Start:	47,376,409 bp from pter	End:	47,400,127 bp from pter
Size:	23,719 bases	Orientation:	minus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: SPI1_HUMAN, P17947 (See protein sequence)

Recommended Name: Transcription factor PU.1

Size: 270 amino acids; 31083 Da

Subunit: Binds DNA as a monomer. Interacts with CEBPD and NONO (By similarity). Interacts with RUNX1 and SPIB.

Interacts with GFI1; the interaction represses SPI1 transcriptional activity

Subcellular location: Nucleus

Sequence caution: Sequence=CAA36281.1; Type=Erroneous initiation; Note=Translation N-terminally extended;

Alternative splicing: 2 isoforms: P17947-1 P17947-2

Explore the universe of human proteins at neXtProt for SPI1: NX_P17947

Post-translational modifications:

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_P17947

SPI1 Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins (2 alternative transcripts):

NP_001074016.1 NP_003111.2

ENSEMBL proteins:

ENSP00000367799 ENSP00000227163 ENSP00000443865 ENSP00000438846

Human Recombinant Protein Products for SPI1:

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	GenScript Custom Purified and Recombinant Proteins Services for SPI1
	Novus Biologicals SPI1 Proteins Novus Biologicals SPI1 Lysates
	Browse Sino Biological Recombinant Proteins
	Browse ProSpec Recombinant Proteins
	Uscn Proteins for SPI1

Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0000790	nuclear chromatin	IDA	15304486
GO:0005634	nucleus	--	--

SPI1 for ontologies About GeneDecksing

SPI1 Antibody Products:

	EMD Millipore Mono- and Polyclonal Antibodies for the study of SPI1
	R&D Systems Antibodies for SPI1 (PU.1/Spi-1)
	Cell Signaling Technology (CST) Antibodies for SPI1 (PU.1)
	OriGene Antibodies (see all 3): SPI1 OriGene Custom Antibody Services for SPI1
	GenScript Superior Antibodies for SPI1
	Novus Biologicals SPI1 Antibodies
	Abcam antibodies for SPI1
	Uscn Antibodies for SPI1
	ThermoFisher Antibody for SPI1

Assay Products for SPI1:

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Immunoassay Development Browse OriGene Fluorogenic Cell Assay Kits
	Browse R&D Systems for biochemical assays
	GenScript Custom Assay Services for SPI1
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for SPI1

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

SPI1 for domains About GeneDecksing

2 InterPro domains/families:

IPR011991 WHTH_trsnscrt_rep_DNA-bd_dom

IPR000418 Ets_dom

Graphical View of Domain Structure for InterPro Entry P17947

ProtoNet protein and cluster: P17947

UniProtKB/Swiss-Prot: SPI1_HUMAN, P17947

Similarity: Belongs to the ETS family

Similarity: Contains 1 ETS DNA-binding domain

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, Sirion Biotech, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, Sirion Biotech, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Molecular Function:

UniProtKB/Swiss-Prot Summary: SPI1_HUMAN, P17947

Function: Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer.

This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of

macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity)

Induction: Highly expressed in both FV-P and FV-A-induced erythro-leukemia cell lines that have undergone

rearrangements of the SPI1 gene due to the insertion of SFFV

Genatlas biochemistry entry for SPI1:

spleen focus forming virus (SFFV) proviral integration oncogene spi1,Pu-1 homolog,expressed in various hemapoietic cell

lines,required for the development of both myeloid and lymphoid lineages

Gene Ontology (GO): 5/11 molecular function terms (GO ID links to tree view) (see all 11): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0000980	RNA polymerase II distal enhancer sequence-specific DNA binding	ISS	--
GO:0001047	core promoter binding	IDA	--
GO:0001085	RNA polymerase II transcription factor binding	ISS	--
GO:0001205	RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription	IEA	--
GO:0003677	DNA binding	--	--

SPI1 for ontologies About GeneDecksing

Phenotypes:
3 GenomeRNAi human phenotypes for SPI1:

Decreased NANOG protein expres

Decreased OCT4 protein express

Decreased POU5F1-GFP protein e

14 MGI mutant phenotypes (inferred from 16 alleles) (MGI details for Sfpi1):

cellular	craniofacial	growth/size	hematopoietic system	homeostasis/metabolism
immune system	liver/biliary system	mortality/aging	nervous system	no phenotypic analysis
normal	skeleton	tumorigenesis	vision/eye

SPI1 for phenotypes           About GeneDecksing

Animal Models:
     Mouse knock-outs for SPI1: Sfpi1^tm2.1Dgt Sfpi1^tm1Rpd Sfpi1^tm1.3Nutt Sfpi1^tm1.1Rpd Sfpi1^tm1.3Dgt Sfpi1^tm1Ram
                                               Sfpi1^tm1.1Dgt Sfpi1^tm1Sing

inGenious Targeting Laboratory - Customizable classic, inducible, and humanized mouse model solutions for SPI1

miRNA Products:		miRTarBase miRNAs that target SPI1: hsa-mir-569 (MIRT004827), hsa-mir-424 (MIRT005928), hsa-mir-34a (MIRT005724), hsa-mir-155 (MIRT004282)

		OriGene 3'-UTR Clone (see all 2): SPI1 Browse MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat SPI1
		6 QIAGEN miScript miRNA Assays for microRNAs that regulate SPI1:
		hsa-miR-1538 hsa-miR-155 hsa-miR-1910 hsa-miR-31* hsa-miR-1289 hsa-miR-3657
		SwitchGear 3'UTR luciferase reporter plasmid: SPI1 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for SPI1 (see all 4) OriGene shRNA RFP: SPI1 OriGene siRNA: SPI1
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat SPI1
		Sirion Biotech Custom design and validation of potent shRNA sequences against SPI1

Gene Editing Products:			DNA2.0 Custom Protein Engineering Service for SPI1
			Sirion Biotech Customized adenovirus for overexpression of SPI1 Sirion Biotech Customized adenovirus for potent knockdown of SPI1

Clone Products:		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for SPI1 (see all 3) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for SPI1 (see all 3) OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector (see all 2): SPI1 (NM_003120)
		Browse Sino Biological Human cDNA Clones
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for SPI1
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat SPI1

Cell Line Products:		GenScript Custom overexpressing Cell Line Services for SPI1
		Search LifeMap BioReagents cell lines for SPI1
		Sirion Biotech Customized stable knockdown cell line services for SPI1 Sirion Biotech Customized inducible knockdown cell line services for SPI1 Sirion Biotech Customized stable overexpressing cell line services for SPI1 Sirion Biotech Customized inducible overexpressing cell line services for SPI1

In Situ Assay
Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for SPI1

(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
About This Section

Unified GeneCards pathways - 5/15 super-pathways (see all 15) About this table

See pathways by source

Super-pathway

contained gene-specific pathways

Toll Comparative Pathway

Immune response MIF in innate immunity response

0.46

Immune response_MIF in innate immunity response

0.46

Lymphocyte Signaling

Lymphocyte Signaling

1.00

Type II interferon signaling (IFNG)

Type II interferon signaling (IFNG)

1.00

IL-3 Signaling Pathway

IL-3 Signaling Pathway

1.00

IL-4 signaling Pathway

IL-4 signaling Pathway

1.00

Pathway sources
See GeneCards unified pathways
Show all pathways

1 EMD Millipore Pathway for SPI1

Immune response MIF in innate immunity response

1 R&D Systems Pathway for SPI1

IL-4 Signaling and its Primary Biological Effects in Different Immune Cell Types

1 Cell Signaling Technology (CST) Pathway for SPI1

Lymphocyte Signaling

1 GeneGo (Thomson Reuters) Pathway for SPI1

Immune response MIF in innate immunity response

5/9 BioSystems Pathways for SPI1 (see all 9)

	Type II interferon signaling (IFNG)
	IL-4 signaling Pathway
	RANKL/RANK Signaling Pathway
	IL-3 Signaling Pathway
	Regulation of retinoblastoma protein

3 Kegg Pathways (Kegg details for SPI1):

	Osteoclast differentiation
	Pathways in cancer
	Acute myeloid leukemia

SPI1 for pathways About GeneDecksing

Interactions:

SABiosciences Gene Network Central^TM Interacting Genes and Proteins Network for SPI1

STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

5/86 Interacting proteins for SPI1 (P17947^{1, 3} ENSP00000227163⁴) via UniProtKB, MINT, STRING, and/or I2D (see all 86)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
HOXA10	P31260¹^,³, ENSP00000283921⁴	EBI-2293548,EBI-2293516 I2D: score=3 STRING: ENSP00000283921
JUN	P05412³, ENSP00000360266⁴	I2D: score=5 STRING: ENSP00000360266
IRF8	Q02556³, ENSP00000268638⁴	I2D: score=4 STRING: ENSP00000268638
NONO	Q15233³, ENSP00000276079⁴	I2D: score=4 STRING: ENSP00000276079
ATF1	P18846³, ENSP00000262053⁴	I2D: score=3 STRING: ENSP00000262053

About this table

Gene Ontology (GO): 5/23 biological process terms (GO ID links to tree view) (see all 23): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0000122	negative regulation of transcription from RNA polymerase II promoter	TAS	10867017
GO:0001944	vasculature development	IEA	--
GO:0002320	lymphoid progenitor cell differentiation	IEA	--
GO:0002573	myeloid leukocyte differentiation	--	--
GO:0006355	regulation of transcription, DNA-dependent	--	--

SPI1 for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section

SPI1 for compounds About GeneDecksing

Browse Small Molecules at EMD Millipore

Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for SPI1
6 Novoseek chemical compound relationships for SPI1 gene About this table

Compound	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
5-aza-2'deoxycytidine	21.6	2	16304050 (1)
tyrosine	20.7	6	18794139 (1), 7478542 (1), 17361223 (1), 11865067 (1)
leucine	10.7	1	16931285 (1)
serine	5.61	4	11432724 (2), 11823535 (1)
proline	0	2	11823535 (1)
glutamate	0	2	11823535 (1)

Search CenterWatch for drugs/clinical trials and news about SPI1

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from EMD Millipore,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, Sirion Biotech, microRNA from QIAGEN,
Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
About This Section

REFSEQ mRNAs for SPI1 gene (2 alternative transcripts):

NM_001080547.1 NM_003120.2

Unigene Cluster for SPI1:

Spleen focus forming virus (SFFV) proviral integration oncogene spi1

Hs.502511 [show with all ESTs]

Unigene Representative Sequence: NM_001080547

4 Ensembl transcripts including schematic representations, and UCSC links where relevant:

ENST00000378538(uc001nfb.1 uc001nfc.1) ENST00000227163 ENST00000533030

ENST00000533968(uc009ylp.1)

miRNA Products:		OriGene 3'-UTR Clone (see all 2): SPI1 Browse OriGene MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat SPI1
		6 QIAGEN miScript miRNA Assays for microRNAs that regulate SPI1:
		hsa-miR-1538 hsa-miR-155 hsa-miR-1910 hsa-miR-31* hsa-miR-1289 hsa-miR-3657
		SwitchGear 3'UTR luciferase reporter plasmid: SPI1 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for SPI1 (see all 4) OriGene shRNA RFP: SPI1 OriGene siRNA: SPI1
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat SPI1
		Sirion Biotech Custom design and validation of potent shRNA sequences against SPI1

Clone Products:		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for SPI1 (see all 3) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for SPI1 (see all 3) OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector (see all 2): SPI1 (NM_003120)
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for SPI1
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat SPI1

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for SPI1 Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat SPI1
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat SPI1
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat SPI1

Additional cDNA sequence:

AK310065.1 X52056.1

7 DOTS entries:

DT.212447 DT.100814731 DT.86855596 DT.91688064 DT.91688065 DT.92034201 DT.92059421

24/114 AceView cDNA sequences (see all 114):

CR613003 BQ231837 BM701185 CR625357 BI909431 CR615071 BI909430 BM726188

CR599683 BM715710 BI760638 CD369819 NM_003120 AL547842 CA308306 BM994691

BM919205 CD370153 BI906692 BI518561 AI759981 CD743247 BI908495 BP432550

GeneLoc Exon Structure

3 Alternative Splicing Database (ASD) splice patterns (SP) for SPI1 About this scheme

ExUns:	1	^	2	^	3a	·	3b	^	4	^	5a	·	5b	^	6	^	7a	·	7b
SP1:
SP2:
SP3:					-		-

ECgene alternative splicing isoforms for SPI1

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

SPI1 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: AAGGACAGCA

About this image

SPI1 expression in embryonic tissues and stem cells
Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine About this table

10 LifeMap In Vivo Development Anatomical Compartments/Cells

Tissue

Anatomical Compartment

Cell

Category (developmental path)

Blood

Peripheral Blood

Common Lymphoid Progenitor Cells

Selective marker (cell-identifying gene)

Blood

Peripheral Blood

Common Myeloid Progenitor Cells

Blood

Peripheral Blood

B-cell Progenitor Cells

Blood

Blood

Blood

Blood

Kidney

Kidney

Kidney

Kidney

Expression:

Positive

Negative

Selective marker

Experimental details:

Curated

Microarrays

In-situ hybridization

See SPI1 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for SPI1

SOURCE GeneReport for Unigene cluster: Hs.502511

SABiosciences Expression via Pathway-Focused PCR Arrays including SPI1:

Leukemia in human mouse rat

JAK / STAT Signaling Pathway in human mouse rat

Allergy & Asthma in human mouse rat

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for SPI1 Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat SPI1
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat SPI1
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat SPI1

In Situ
Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for SPI1

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the common ancestor of chordates.

Orthologs for SPI1 gene from 3/12 species (see all 12) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
chicken (Gallus gallus)	Aves	SPI1¹	spleen focus forming virus (SFFV) proviral integration oncogene spi1 less	77.73(n) 83.2(a)		395879 NM_205023.1 NP_990354.1
lizard (Anolis carolinensis)	Reptilia	SPI1⁶	--	71(a)	1 ↔ 1	1(44015193-44030760)
zebrafish (Danio rerio)	Actinopterygii	spi1²	spi1	75.2(n)		30117 BC056282.1

ENSEMBL Gene Tree for SPI1 (if available)
TreeFam Gene Tree for SPI1 (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
About This Section

Paralogs for SPI1 gene

SPIB² SPIC²

2 SIMAP similar genes for SPI1 using alignment to 3 protein entries: SPI1_HUMAN (see all proteins):

SPIC SPIB

SPI1 for paralogs About GeneDecksing

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section

SNP ID	Valid	Clinical significance	Chr 11 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs149436187^1,2		--	47376342(+)	`AGAGAC/TGTCGC`	2	--	ds500¹		0	--	--	--	--
rs3740686^1,2	C,F,A,H	--	47376363(+)	`GGCCAG/AAAGAG`	2	--	ds500¹		12		EA NA WA CSA	1673
rs116330587^1,2		--	47376421(+)	`TGAGAA/GTAACT`	2	--	ut3¹		1		WA	118
rs182746140^1,2		--	47376446(+)	`GGAGGA/CGGTTA`	2	--	ut3¹		0	--	--	--	--
rs1057233^1,2	C,F,A,H	--	47376448(-)	`ATTAAT/CCTCCT`	2	--	ut3¹		13		EA NS NA WA CSA	2063
rs10747^1,2		--	47376544(+)	`TGCCCC/GGGTGG`	2	--	ut3¹ ese³		1		MN	184
rs142654617^1,2	C,F	--	47376862(+)	`TTCACC/TTTCTT`	4	K	syn¹		1		NA	4488
rs150932236^1,2	C	--	47376874(+)	`ACCTCG/TCCCGT`	4	G	syn¹		1		NA	4526
rs150085664^1,2	C,F	--	47376927(+)	`CATCTT/GCTTGC`	4	/K /Q	mis¹		2		NA EU	5855
rs113063744^1,2	C,F	--	47376967(+)	`GCCAGC/GGCCTC`	4	A	syn¹		2		CSA NA	4547

HapMap Linkage Disequilibrium report for SPI1 (47376409 - 47400127 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 3 variations for SPI1
2 CNVs: 85757 75634
1 Indel: 85758
Human Gene Mutation Database (HGMD): SPI1

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(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

SPI1 for disorders           About GeneDecksing

OMIM gene information: 165170    OMIM disorders: --

20/27 diseases for SPI1 (see all 27):    About MalaCards

pulmonary alveolar proteinosis non-hodgkin lymphoma typhoid fever stem cell leukemia

primary effusion lymphoma b-cell lymphomas myeloid leukemia plasmacytoma

acute promyelocytic leukemia hodgkin's lymphoma gastroenteritis microphthalmia

chronic lymphocytic leukemia leukemia acute myeloid leukemia chronic myeloid leukemia

lymphocytic leukemia multiple myeloma t-cell leukemia mediastinitis

1 disease from the University of Copenhagen DISEASES database for SPI1:

Leukemia

10/14 Novoseek disease relationships for SPI1 gene (see all 14) About this table

Disease	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
erythroleukemia	72.5	12	8502483 (3), 2338340 (2), 1620109 (1), 19955566 (1) (see all 9)
leukemogenesis	40.6	4	17361223 (1), 15910240 (1)
microphthalmia	40.2	3	15304486 (1), 16109714 (1), 19801646 (1)
leukemia	38.7	6	16909135 (1), 16735999 (1), 11841448 (1), 16158816 (1) (see all 6)
hodgkin disease	28.3	9	15339679 (4), 11696441 (1), 16648862 (1), 15796964 (1)
myeloid leukemia	27	4	9826539 (1), 12393465 (1)
lymphoma b-cell	20.4	6	15339679 (4), 12507907 (1), 15202521 (1)
plasmacytoma	18.3	2	7670114 (1)
lymphoma	18.2	1	19828640 (1)
leukemia t-cell	14.5	1	9376596 (1)

Human Genome Epidemiology (HuGE) Navigator: SPI1 (4 documents)
Tumor Gene Database (TGDB): SPI1

Export disorders for SPI1 gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
About This Section

PubMed articles for SPI1 gene, integrated from 9 sources (see all 259):
(articles sorted by number of sources associating them with SPI1)

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world of online information

The human homologue of the putative proto-oncogene Spi-1: characterization and expression in tumors. (PubMed id 1693183)^{1, 2, 3, 9} Ray D.... Moreau-Gachelin F. (1990)
Functional and physical interactions between AML1 proteins and an ETS protein, MEF: implications for the pathogenesis of t(8;21)-positive leukemias. (PubMed id 10207087)^{1, 2} Mao S.... Nimer S.D. (1999)
SPI-B activates transcription via a unique proline, serine, and threonine domain and exhibits DNA binding affinity differences from PU.1. (PubMed id 10196196)^{1, 2} Rao S.... Simon M.C. (1999)
The myeloid master regulator transcription factor PU.1 is inactivated by AML1-ETO in t(8;21) myeloid leukemia. (PubMed id 12393465)^{1, 9} Vangala R.K....Behre G. (2003)
Roles of PU.1 in monocyte- and mast cell-specific gen e regulation: PU.1 transactivates CIITA pIV in cooperation with IFN-gamma. (PubMed id 19502584)^{1, 9} Ito T....Okumura K. (2009)
Carrier-independent nuclear import of the transcription factor PU.1 via RanGTP-stimulated binding to Nup153. (PubMed id 15632149)^{1, 9} Zhong H....Yaseen N.R. (2005)
Negative cross-talk between hematopoietic regulators: GATA proteins repress PU.1. (PubMed id 10411939)^{1, 9} Zhang P....Sun Z. (1999)
PU.1 regulates the tissue-specific expression of dendritic cell-specific intercellular adhesion molecule (ICAM)-3-grabbing nonintegrin. (PubMed id 16051608)^{1, 9} Dominguez-Soto A....Corbi A.L. (2005)
Localization of the human oncogene SPI1 on chromosome 11, region p11.22. (PubMed id 2338340)^{1, 9} Nguyen V.C....Moreau-Gachelin F. (1990)
Human T-cell leukemia virus type I Tax transactivates the promoter of human prointerleukin-1beta gene through association with two transcription factors, nuclear factor-interleukin-6 and Spi-1. (PubMed id 9376596)^{1, 9} Tsukada J....Eto S. (1997)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

		AND	OR
Aliases
Disorders
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section

Entrez Gene: 6688	HGNC: 11241	AceView: SPI1	Ensembl:ENSG00000066336	euGenes: HUgn6688
ECgene: SPI1	Kegg: 6688	H-InvDB: SPI1

(According to HUGE)
About This Section

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Name	Description
PharmGKB entry for SPI1	Pharmacogenomics, SNPs, Pathways
ATLAS Chromosomes in Cancer entry for SPI1	Genetics and Cytogenetics in Oncology and Haematology

Intellectual Property for SPI1 gene
(Patent information from GeneIP, Licensable technologies from WIS Yeda, Salk, Tufts, IP news from LifeMap Sciences, Inc.)

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Patent Information for SPI1 gene:
Search GeneIP for patents involving SPI1

GeneCards and IP:
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