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Aliases for SPHK2 Gene

Aliases for SPHK2 Gene

  • Sphingosine Kinase 2 2 3 5
  • SPK 2 3 4
  • SK 2 3 4
  • Sphingosine Kinase Type 2 3
  • EC 2.7.1.91 4
  • SPK-2 3
  • SK-2 3

External Ids for SPHK2 Gene

Previous GeneCards Identifiers for SPHK2 Gene

  • GC19P049767
  • GC19P049490
  • GC19P053798
  • GC19P053814
  • GC19P049122
  • GC19P045501

Summaries for SPHK2 Gene

Entrez Gene Summary for SPHK2 Gene

  • This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]

GeneCards Summary for SPHK2 Gene

SPHK2 (Sphingosine Kinase 2) is a Protein Coding gene. Among its related pathways are Metabolism and sphingosine and sphingosine-1-phosphate metabolism. GO annotations related to this gene include NAD+ kinase activity and diacylglycerol kinase activity. An important paralog of this gene is SPHK1.

UniProtKB/Swiss-Prot for SPHK2 Gene

  • Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides.

Tocris Summary for SPHK2 Gene

  • Sphingosine kinase (SphK) mediates the conversion of sphingosine to sphingosine-1-phosphate, a key sphingolipid signaling molecule involved in cell growth, survival, differentiation and motility. Two mammalian isoforms have been cloned - SPHK1 and SPHK2.

Gene Wiki entry for SPHK2 Gene

No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for SPHK2 Gene

Genomics for SPHK2 Gene

Regulatory Elements for SPHK2 Gene

Enhancers for SPHK2 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH19F048588 1.7 FANTOM5 Ensembl ENCODE 2.6 -30.1 -30084 1.2 HDGF PKNOX1 ARNT ARID4B SIN3A YBX1 ZNF2 CBX5 ZNF416 ZNF143 GRWD1 SNORD35A SULT2B1 KDELR1 GLTSCR2-AS1 ALDH16A1 GLTSCR1 SNORD35B EHD2 SNORD23
GH19F048283 0.9 ENCODE 4.6 -334.9 -334909 2.0 HDGF ATF1 CBX3 TBL1XR1 CREB3L1 SIN3A ARID4B NFXL1 ZNF2 ZSCAN9 CCDC114 SYNGR4 TMEM143 LMTK3 IZUMO1 GRIN2D SPHK2 GC19P048288 LOC105372429
GH19F048988 1.8 FANTOM5 Ensembl ENCODE 1.3 +372.8 372763 7.2 MLX CREB3L1 ZFP64 DMAP1 FEZF1 YY1 SLC30A9 ZNF143 ZNF263 SP3 GRWD1 TEAD2 NUP62 AKT1S1 KDELR1 ENSG00000268157 SNORD35A LOC101059948 PLEKHA4 PRR12
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around SPHK2 on UCSC Golden Path with GeneCards custom track

Genomic Location for SPHK2 Gene

Chromosome:
19
Start:
48,619,291 bp from pter
End:
48,630,717 bp from pter
Size:
11,427 bases
Orientation:
Plus strand

Genomic View for SPHK2 Gene

Genes around SPHK2 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
SPHK2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for SPHK2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for SPHK2 Gene

Proteins for SPHK2 Gene

  • Protein details for SPHK2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9NRA0-SPHK2_HUMAN
    Recommended name:
    Sphingosine kinase 2
    Protein Accession:
    Q9NRA0
    Secondary Accessions:
    • A0T4C8
    • B4DU87
    • Q9BRN1
    • Q9H0Q2
    • Q9NWU7

    Protein attributes for SPHK2 Gene

    Size:
    654 amino acids
    Molecular mass:
    69217 Da
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    No Data Available

    Alternative splice isoforms for SPHK2 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for SPHK2 Gene

Post-translational modifications for SPHK2 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

No data available for DME Specific Peptides for SPHK2 Gene

Domains & Families for SPHK2 Gene

Gene Families for SPHK2 Gene

Protein Domains for SPHK2 Gene

Suggested Antigen Peptide Sequences for SPHK2 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q9NRA0

UniProtKB/Swiss-Prot:

SPHK2_HUMAN :
  • Contains 1 DAGKc domain.
Domain:
  • Contains 1 DAGKc domain.
genes like me logo Genes that share domains with SPHK2: view

Function for SPHK2 Gene

Molecular function for SPHK2 Gene

UniProtKB/Swiss-Prot BiophysicochemicalProperties:
Kinetic parameters: KM=3.4 uM for sphingosine {ECO:0000269 PubMed:10751414}; pH dependence: Optimum pH is 7.5. {ECO:0000269 PubMed:10751414};
UniProtKB/Swiss-Prot CatalyticActivity:
ATP + a sphingoid base = ADP + a sphingoid base 1-phosphate.
UniProtKB/Swiss-Prot EnzymeRegulation:
Inhibited by sulfatide.
UniProtKB/Swiss-Prot Function:
Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides.

Enzyme Numbers (IUBMB) for SPHK2 Gene

Gene Ontology (GO) - Molecular Function for SPHK2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005515 protein binding IPI 11777919
GO:0005524 ATP binding IEA --
GO:0008481 sphinganine kinase activity TAS --
GO:0016301 kinase activity IEA --
GO:0016740 transferase activity IEA --
genes like me logo Genes that share ontologies with SPHK2: view
genes like me logo Genes that share phenotypes with SPHK2: view

Animal Models for SPHK2 Gene

MGI Knock Outs for SPHK2:

Animal Model Products

miRNA for SPHK2 Gene

miRTarBase miRNAs that target SPHK2

Flow Cytometry Products

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for SPHK2 Gene

Localization for SPHK2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for SPHK2 Gene

Isoform 1: Cytoplasm. Membrane.
Isoform 2: Lysosome membrane.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for SPHK2 gene
Compartment Confidence
cytosol 5
lysosome 5
nucleus 3
plasma membrane 1
extracellular 1
mitochondrion 1
endoplasmic reticulum 1

Gene Ontology (GO) - Cellular Components for SPHK2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm IEA --
GO:0005764 lysosome IEA --
GO:0005765 lysosomal membrane IEA,IDA 17897319
GO:0005829 cytosol TAS --
GO:0016020 membrane IEA,IDA 10751414
genes like me logo Genes that share ontologies with SPHK2: view

Pathways & Interactions for SPHK2 Gene

genes like me logo Genes that share pathways with SPHK2: view

SIGNOR curated interactions for SPHK2 Gene

Is activated by:

Gene Ontology (GO) - Biological Process for SPHK2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001568 blood vessel development IEA --
GO:0003376 sphingosine-1-phosphate signaling pathway IEA --
GO:0006669 sphinganine-1-phosphate biosynthetic process NAS 10751414
GO:0006670 sphingosine metabolic process IEA --
GO:0007420 brain development IEA --
genes like me logo Genes that share ontologies with SPHK2: view

Drugs & Compounds for SPHK2 Gene

(13) Drugs for SPHK2 Gene - From: ApexBio, DGIdb, HMDB, Tocris, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Adenosine triphosphate Approved Nutra 0
Sphingosine Experimental Pharma 0
Phenoxodiol Investigational Pharma inhibitor 0
ABC294640 Pharma Sphingosine kinase 2 inhibitor,selective and competitive 0
SKI II Pharma Sphingosine kinase(SK) inhibitor, Selective non-lipid inhibitor of sphingosine kinase 0

(4) Additional Compounds for SPHK2 Gene - From: HMDB and Tocris

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
ADP
  • Adenosindiphosphorsaeure
  • Adenosine 5'-pyrophosphate
  • Adenosine diphosphate
  • Adenosine pyrophosphate
  • Adenosine-5'-diphosphate
Full agonist, Agonist 58-64-0
sphinganine 1-phosphate
  • 2-Amino-3-hydroxyoctadecyl dihydrogen phosphate
  • Dihydrosphingosine 1-phosphate
  • Dihydrosphingosine-1-phosphate
  • Sphinganine 1-phosphate
19794-97-9
MP A08
219832-49-2
PF 543 hydrochloride
1706522-79-3

(4) Tocris Compounds for SPHK2 Gene

Compound Action Cas Number
MP A08 Selective ATP competitive SphK1 and 2 inhibitor; cell permeable 219832-49-2
N,N-Dimethylsphingosine Sphingosine kinase inhibitor 119567-63-4
PF 543 hydrochloride Potent and selective SphK1 inhibitor 1706522-79-3
SKI II Selective non-lipid inhibitor of sphingosine kinase 312636-16-1

(4) ApexBio Compounds for SPHK2 Gene

Compound Action Cas Number
ABC294640 Sphingosine kinase 2 inhibitor,selective and competitive 915385-81-8
K145 SphK2 inhibitor 1309444-75-4
K145 hydrochloride 1449240-68-9
SKI II Sphingosine kinase(SK) inhibitor 312636-16-1
genes like me logo Genes that share compounds with SPHK2: view

Transcripts for SPHK2 Gene

mRNA/cDNA for SPHK2 Gene

Unigene Clusters for SPHK2 Gene

Sphingosine kinase 2:
Representative Sequences:

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for SPHK2 Gene

ExUns: 1 ^ 2a · 2b · 2c · 2d · 2e ^ 3a · 3b ^ 4 ^ 5a · 5b · 5c ^ 6 ^ 7a · 7b · 7c ^ 8 ^ 9 ^ 10a · 10b · 10c ^ 11 ^ 12 ^ 13a · 13b · 13c ^
SP1: - - - - - - - - - - - -
SP2: - - - - - - - - - -
SP3: -
SP4: - - - - - - - - - - - - - - -
SP5: - - - - - - - - - - - - - - - -
SP6: - - - - - - - - - - - - -
SP7: - - - - - - - - - - - - - -
SP8:
SP9:
SP10: - - - - - - - - - - - - - - - -
SP11: - - - - - - - -
SP12: - - - -
SP13:
SP14: - - - - - - -
SP15: - - - - - - - -
SP16: - - - - - - - -
SP17: - - - - - -
SP18: - - -
SP19: - -
SP20: - - -

ExUns: 14 ^ 15a · 15b · 15c · 15d ^ 16a · 16b ^ 17a · 17b ^ 18
SP1: -
SP2: -
SP3: -
SP4:
SP5: -
SP6: -
SP7:
SP8: -
SP9: - - -
SP10:
SP11:
SP12:
SP13: - - -
SP14:
SP15:
SP16:
SP17:
SP18:
SP19:
SP20:

Relevant External Links for SPHK2 Gene

GeneLoc Exon Structure for
SPHK2
ECgene alternative splicing isoforms for
SPHK2

Expression for SPHK2 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for SPHK2 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for SPHK2 Gene

This gene is overexpressed in Frontal cortex (27.9), Spinal cord (18.0), and Testis (15.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for SPHK2 Gene



Protein tissue co-expression partners for SPHK2 Gene

NURSA nuclear receptor signaling pathways regulating expression of SPHK2 Gene:

SPHK2

SOURCE GeneReport for Unigene cluster for SPHK2 Gene:

Hs.528006
genes like me logo Genes that share expression patterns with SPHK2: view

No data available for mRNA differential expression in normal tissues and mRNA Expression by UniProt/SwissProt for SPHK2 Gene

Orthologs for SPHK2 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for SPHK2 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia SPHK2 34 35
  • 97.49 (n)
dog
(Canis familiaris)
Mammalia SPHK2 34 35
  • 87.77 (n)
platypus
(Ornithorhynchus anatinus)
Mammalia SPHK2 35
  • 86 (a)
OneToOne
cow
(Bos Taurus)
Mammalia SPHK2 34 35
  • 85.58 (n)
mouse
(Mus musculus)
Mammalia Sphk2 34 16 35
  • 80.92 (n)
rat
(Rattus norvegicus)
Mammalia Sphk2 34
  • 80.67 (n)
lizard
(Anolis carolinensis)
Reptilia SPHK2 35
  • 55 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia sphk2 34
  • 57.41 (n)
zebrafish
(Danio rerio)
Actinopterygii sphk2 34 35
  • 58.51 (n)
Dr.12967 34
fruit fly
(Drosophila melanogaster)
Insecta Sk1 36 34 35
  • 49.18 (n)
Sk2 35
  • 29 (a)
ManyToMany
worm
(Caenorhabditis elegans)
Secernentea sphk-1 34 35
  • 40.16 (n)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes LCB4 35
  • 19 (a)
ManyToMany
LCB5 35
  • 18 (a)
ManyToMany
sea squirt
(Ciona savignyi)
Ascidiacea -- 35
  • 32 (a)
OneToMany
Species where no ortholog for SPHK2 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • oppossum (Monodelphis domestica)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for SPHK2 Gene

ENSEMBL:
Gene Tree for SPHK2 (if available)
TreeFam:
Gene Tree for SPHK2 (if available)

Paralogs for SPHK2 Gene

Paralogs for SPHK2 Gene

(2) SIMAP similar genes for SPHK2 Gene using alignment to 7 proteins:

genes like me logo Genes that share paralogs with SPHK2: view

Variants for SPHK2 Gene

Sequence variations from dbSNP and Humsavar for SPHK2 Gene

SNP ID Clin Chr 19 pos Sequence Context AA Info Type
rs10422714 -- 48,622,276(+) aataa(A/T)aaaaa intron-variant
rs10423867 -- 48,622,026(+) cgaga(C/T)gggcg intron-variant
rs111252148 -- 48,622,994(+) AATCC(C/T)AGCAC intron-variant, upstream-variant-2KB
rs111343257 -- 48,621,708(+) ATTTC(A/G)TGGGG intron-variant
rs111579309 -- 48,627,085(+) GCCGA(A/G)ATTGC intron-variant

Structural Variations from Database of Genomic Variants (DGV) for SPHK2 Gene

Variant ID Type Subtype PubMed ID
dgv3617n100 CNV gain 25217958
esv1003432 CNV gain 20482838
nsv9739 CNV gain+loss 18304495

Variation tolerance for SPHK2 Gene

Residual Variation Intolerance Score: 37.2% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 4.38; 63.50% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for SPHK2 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
SPHK2

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for SPHK2 Gene

Disorders for SPHK2 Gene

Relevant External Links for SPHK2

Human Genome Epidemiology (HuGE) Navigator
SPHK2
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
SPHK2

No disorders were found for SPHK2 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for SPHK2 Gene

Publications for SPHK2 Gene

  1. Molecular cloning and functional characterization of a novel mammalian sphingosine kinase type 2 isoform. (PMID: 10751414) Liu H. … Spiegel S. (J. Biol. Chem. 2000) 2 3 4 22 64
  2. A lipid binding domain in sphingosine kinase 2. (PMID: 19168031) Don A.S. … Rosen H. (Biochem. Biophys. Res. Commun. 2009) 3 4 22 64
  3. Cleavage of sphingosine kinase 2 by caspase-1 provokes its release from apoptotic cells. (PMID: 20197547) Weigert A. … BrA1ne B. (Blood 2010) 3 22 64
  4. Sphingosine kinase isoforms regulate oxaliplatin sensitivity of human colon cancer cells through ceramide accumulation and Akt activation. (PMID: 19240026) Nemoto S. … Banno Y. (J. Biol. Chem. 2009) 3 22 64
  5. Expression of sphingosine kinase 2 in synovial fibroblasts of rheumatoid arthritis contributing to apoptosis by a sphingosine analogue, FTY720. (PMID: 19490468) Kamada K. … Nose M. (Pathol. Int. 2009) 3 22 64

Products for SPHK2 Gene

Sources for SPHK2 Gene

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