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SPHK2 Gene

protein-coding   GIFtS: 66
GCID: GC19P049122

Sphingosine Kinase 2

  See related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Sphingosine Kinase 21 2     SPK-22
SK 22 3     Sphingosine Kinase Type 22
SPK 22 3     EC 2.7.1.913
SK-22     

External Ids:    HGNC: 188591   Entrez Gene: 568482   Ensembl: ENSG000000631767   OMIM: 6070925   UniProtKB: Q9NRA03   

Export aliases for SPHK2 gene to outside databases

Previous GC identifers: GC19P049767 GC19P049490 GC19P053798 GC19P053814 GC19P045501


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for SPHK2 Gene:
This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into
sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration,
proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and
tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance.
Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. (provided
by RefSeq, Aug 2011)

GeneCards Summary for SPHK2 Gene:
SPHK2 (sphingosine kinase 2) is a protein-coding gene. Diseases associated with SPHK2 include lymphopenia, and keloids. GO annotations related to this gene include NAD+ kinase activity and Ras GTPase binding. An important paralog of this gene is CERKL.

UniProtKB/Swiss-Prot: SPHK2_HUMAN, Q9NRA0
Function: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator
with both intra- and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine
and L-threo-dihydrosphingosine. Binds phosphoinositides

summary for SPHK2 Gene:
Sphingosine kinase (SphK) mediates the conversion of sphingosine to sphingosine-1-phosphate, a key
sphingolipid signaling molecule involved in cell growth, survival, differentiation and motility. SphK
constitutes an important checkpoint in the regulation of circulating levels of ceramide, sphingosine and
sphingosine-1-phosphate. Two mammalian isoforms have been cloned - SPHK1 and SPHK2.

Gene Wiki entry for SPHK2 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence:
NC_000019.10  NT_011109.17  NC_018930.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the SPHK2 gene promoter:
         Elk-1   USF1   p53   CREB   deltaCREB   USF-1   ZIC2/Zic2   NF-Y   CBF(2)   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 2): SPHK2 promoter sequence
   Search Chromatin IP Primers for SPHK2

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat SPHK2


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 19q13.2   Ensembl cytogenetic band:  19q13.33   HGNC cytogenetic band: 19q13.33

SPHK2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
SPHK2 gene location

GeneLoc information about chromosome 19         GeneLoc Exon Structure

GeneLoc location for GC19P049122:  view genomic region     (about GC identifiers)

Start:
49,122,548 bp from pter      End:
49,133,974 bp from pter
Size:
11,427 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: SPHK2_HUMAN, Q9NRA0 (See protein sequence)
Recommended Name: Sphingosine kinase 2  
Size: 654 amino acids; 69217 Da
Cofactor: Magnesium (By similarity)
Secondary accessions: A0T4C8 B4DU87 Q9BRN1 Q9H0Q2 Q9NWU7
Alternative splicing: 5 isoforms:  Q9NRA0-1   Q9NRA0-2   Q9NRA0-3   Q9NRA0-4   Q9NRA0-5   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for SPHK2: NX_Q9NRA0

Explore proteomics data for SPHK2 at MOPED

Post-translational modifications: 

  • Modification sites at neXtProt
  • Modification sites at PhosphoSitePlus

  • See SPHK2 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (5 alternative transcripts): 
    NP_001191087.1  NP_001191088.1  NP_001191089.1  NP_001230805.1  NP_064511.2  

    ENSEMBL proteins: 
     ENSP00000245222   ENSP00000471180   ENSP00000470092   ENSP00000469158   ENSP00000410044  
     ENSP00000471111   ENSP00000469448   ENSP00000470679   ENSP00000471205   ENSP00000472983  
     ENSP00000413369   ENSP00000341091  
    Reactome Protein details: Q9NRA0

    SPHK2 Human Recombinant Protein Products:

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    Browse OriGene Protein Over-expression Lysates
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    Novus Biologicals SPHK2 Proteins
    Novus Biologicals SPHK2 Lysate
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
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    Cloud-Clone Corp. Proteins for SPHK2

    SPHK2 Antibody Products:

    Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
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    Abcam antibodies for SPHK2
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    Cloud-Clone Corp. CLIAs for SPHK2


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    IUPHAR Guide to PHARMACOLOGY protein family classification: sphingosine kinase 2
    Sphingosine kinase family

    2 InterPro protein domains:
     IPR001206 Diacylglycerol_kinase_cat_dom
     IPR016064 ATP-NAD_kinase_PpnK-typ

    Graphical View of Domain Structure for InterPro Entry Q9NRA0

    ProtoNet protein and cluster: Q9NRA0

    UniProtKB/Swiss-Prot: SPHK2_HUMAN, Q9NRA0
    Similarity: Contains 1 DAGKc domain


    SPHK2 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: SPHK2_HUMAN, Q9NRA0
    Function: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator
    with both intra- and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine
    and L-threo-dihydrosphingosine. Binds phosphoinositides
    Catalytic activity: ATP + sphinganine = ADP + sphinganine 1-phosphate
    Catalytic activity: ATP + sphingosine = ADP + sphingosine 1-phosphate
    Enzyme regulation: Inhibited by sulfatide
    Biophysicochemical properties: Kinetic parameters: KM=3.4 uM for sphingosine; pH dependence: Optimum pH is 7.5;

         Enzyme Number (IUBMB): EC 2.7.1.911

         Gene Ontology (GO): Selected molecular function terms (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003951NAD+ kinase activity IEA--
    GO:0004143diacylglycerol kinase activity IEA--
    GO:0005515protein binding IPI11777919
    GO:0005524ATP binding IEA--
    GO:0008481sphinganine kinase activity NAS10751414
         
    SPHK2 for ontologies           About GeneDecksing


    Phenotypes:
         5 GenomeRNAi human phenotypes for SPHK2:
     Decreased 12E8 tau but not tot  Decreased homologous recombina  Increased S DNA content  Increased cell death HMECs cel 
     Increased telomerase activity 

         11 MGI mutant phenotypes (inferred from 6 alleles(MGI details for Sphk2):
     cardiovascular system  cellular  embryogenesis  hematopoietic system  homeostasis/metabolism 
     immune system  mortality/aging  muscle  nervous system  no phenotypic analysis 
     normal 

    SPHK2 for phenotypes           About GeneDecksing

    Animal Models:
         MGI mouse knock-outs for SPHK2: Sphk2tm1Npa Sphk2tm1.1Cgh Sphk2tm1.1Stlp

       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for SPHK2
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for SPHK2

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for SPHK2
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for SPHK2

    miRNA
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    miRTarBase miRNAs that target SPHK2:
    hsa-mir-149-5p (MIRT045673)

    Block miRNA regulation of human, mouse, rat SPHK2 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate SPHK2 (see all 34):
    hsa-miR-25 hsa-miR-301a hsa-miR-650 hsa-miR-92b hsa-miR-130b hsa-miR-3612 hsa-miR-301b hsa-miR-4324
    SwitchGear 3'UTR luciferase reporter plasmidSPHK2 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for SPHK2
    Predesigned siRNA for gene silencing in human, mouse, rat SPHK2

    Gene Editing
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    DNA2.0 Custom Protein Engineering Service for SPHK2

    Clone
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    OriGene clones in human, mouse for SPHK2 (see all 9)
    OriGene ORF clones in mouse, rat for SPHK2
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    GenScript: all cDNA clones in your preferred vector (see all 4): SPHK2 (NM_001204158)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for SPHK2
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat SPHK2

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    Browse ESI BIO Cell Lines and PureStem Progenitors for SPHK2 
    In Situ Assay
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for SPHK2


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    SPHK2_HUMAN, Q9NRA0: Isoform 1: Cytoplasm. Membrane
    SPHK2_HUMAN, Q9NRA0: Isoform 2: Lysosome membrane
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    cytosol5
    lysosome5
    vacuole5
    nucleus3
    endoplasmic reticulum1
    extracellular1
    plasma membrane1

    Gene Ontology (GO): 3 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005765lysosomal membrane IDA17897319
    GO:0005829cytosol TAS--
    GO:0016020membrane IDA10751414

    SPHK2 for ontologies           About GeneDecksing


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for SPHK2 About   (see all 12)  
    See pathways by source

    SuperPathContained pathways About
    1Sphingolipid metabolism
    Sphingolipid metabolism0.61
    Sphingolipid de novo biosynthesis0.46
    Sphingolipid metabolism0.61
    Sphingolipid Metabolism0.42
    2Metabolism
    Metabolism0.38
    Metabolism of lipids and lipoproteins0.37
    Metabolic pathways0.38
    3Immune response Fc epsilon RI pathway
    VEGF signaling pathway0.39
    VEGF - VEGF R2 Signaling Pathways0.35
    4S1P3 pathway
    Sphingosine 1-phosphate (S1P) pathway0.40
    5IL12-mediated signaling events
    IL12-mediated signaling events0.33

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    1 R&D Systems Pathway for SPHK2
        VEGF - VEGF R2 Signaling Pathways

    1 Downloadable PowerPoint Slide of GeneGlobe Pathway Central Maps for SPHK2
        TNF-Induced Apoptosis Implicating Sphingolipids

    Selected BioSystems Pathways for SPHK2 (see all 6)
        Signal Transduction of S1P Receptor
    Sphingolipid Metabolism
    sphingosine and sphingosine-1-phosphate metabolism
    Sphingosine 1-phosphate (S1P) pathway
    IL12-mediated signaling events

    1 Reactome Pathway for SPHK2
        Sphingolipid de novo biosynthesis


    Selected Kegg Pathways  (Kegg details for SPHK2) (see all 6):
        Sphingolipid metabolism
    Metabolic pathways
    Calcium signaling pathway
    VEGF signaling pathway
    Fc gamma R-mediated phagocytosis


    SPHK2 for pathways           About GeneDecksing

        Pathway & Disease-focused RT2 Profiler PCR Arrays including SPHK2: 
              Hypertension in human mouse rat
              VEGF Signaling in human mouse rat
              Apoptosis in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for SPHK2

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for SPHK2 (Q9NRA03 ENSP000002452224) via UniProtKB, MINT, STRING, and/or I2D (see all 116)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    HIST1H3AP684313, ENSP000003502754I2D: score=1 STRING: ENSP00000350275
    HIST1H3BP684313I2D: score=1 
    HIST1H3CP684313I2D: score=1 
    HIST1H3DP684313I2D: score=1 
    HIST1H3EP684313I2D: score=1 
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 12):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001568blood vessel development IEA--
    GO:0006665sphingolipid metabolic process TAS--
    GO:0006669sphinganine-1-phosphate biosynthetic process NAS10751414
    GO:0006670sphingosine metabolic process IEA--
    GO:0007205protein kinase C-activating G-protein coupled receptor signaling pathway IEA--

    SPHK2 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Compounds for SPHK2 available from Tocris Bioscience    About this table
    CompoundAction CAS #
    N,N-Dimethylsphingosine Sphingosine kinase inhibitor[119567-63-4]
    SK1-ISphingosine kinase 1 (SphK1) inhibitor; antiproliferative[1072443-89-0]

    7 HMDB Compounds for SPHK2    About this table
    CompoundSynonyms CAS #PubMed Ids
    ADPadenosindiphosphorsaeure (see all 8)58-64-0--
    Adenosine triphosphate5'-(tetrahydrogen triphosphate) Adenosine (see all 24)56-65-5--
    MagnesiumMagnesium (see all 2)7439-95-4--
    Sphinganine2-amino-D-erythro-1,3-Octadecanediol (see all 13)764-22-7--
    Sphinganine 1-phosphate2-amino-3-hydroxyoctadecyl dihydrogen phosphate (see all 5)19794-97-9--
    Sphingosine(-)-D-erythro-Sphingosine (see all 7)123-78-4--
    Sphingosine 1-phosphate(2S,3R,4E)-1-(dihydrogen phosphate)2-amino-4-Octadecene-1,3-diol (see all 4)26993-30-6--

    2 Novoseek inferred chemical compound relationships for SPHK2 gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    sphingosine 94.2 58 13129923 (2), 15951439 (2), 19618460 (2), 17311928 (2) (see all 39)
    ceramide 72 7 16118219 (3), 19765607 (1), 19842132 (1), 19240026 (1)



    SPHK2 for compounds           About GeneDecksing



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
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    REFSEQ mRNAs for SPHK2 gene (5 alternative transcripts): 
    NM_001204158.2  NM_001204159.2  NM_001204160.2  NM_001243876.1  NM_020126.4  

    Unigene Cluster for SPHK2:

    Sphingosine kinase 2
    Hs.528006  [show with all ESTs]
    Unigene Representative Sequence: EF107108
    14 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000245222(uc002pjr.3 uc010xzt.2 uc002pjt.3 uc002pjs.3 uc002pjv.3)
    ENST00000601712 ENST00000600537 ENST00000598088 ENST00000426514 ENST00000597434
    ENST00000601704 ENST00000593308 ENST00000599033 ENST00000599748 ENST00000599029
    ENST00000598574 ENST00000443164(uc002pju.3 uc002pjw.3) ENST00000340932(uc010xzu.1)

    miRNA
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    Selected qRT-PCR Assays for microRNAs that regulate SPHK2 (see all 34):
    hsa-miR-25 hsa-miR-301a hsa-miR-650 hsa-miR-92b hsa-miR-130b hsa-miR-3612 hsa-miR-301b hsa-miR-4324
    SwitchGear 3'UTR luciferase reporter plasmidSPHK2 3' UTR sequence
    Inhib. RNA
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    Clone
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    GenScript: all cDNA clones in your preferred vector (see all 4): SPHK2 (NM_001204158)
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    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat SPHK2
    Primer
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    OriGene qPCR primer pairs and template standards for SPHK2
    OriGene qSTAR qPCR primer pairs in human, mouse for SPHK2
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat SPHK2
      QuantiTect SYBR Green Assays in human, mouse, rat SPHK2
      QuantiFast Probe-based Assays in human, mouse, rat SPHK2

    Additional mRNA sequence: 

    AF245447.1 AK000599.1 AK122735.1 AK297787.1 AK300541.1 AL136701.1 BC006161.1 BC010671.1 
    D28468.1 EF107108.1 

    Selected DOTS entries (see all 28):

    DT.91790903  DT.115995  DT.100785328  DT.97822422  DT.95162273  DT.100770536  DT.91777742  DT.100730344 
    DT.121411886  DT.99961873  DT.100766101  DT.121412109  DT.92429390  DT.100780605  DT.121412172  DT.95162274 
    DT.40253235  DT.121411863  DT.121411861  DT.121412180  DT.100766487  DT.100785329  DT.121411865  DT.121412052 

    11 AceView cDNA sequences:

    NM_020126 AK000599 CR614720 AK122735 BC010671 AL136701 BC006161 AB100373 
    AF245447 AK074601 CO246179 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for SPHK2 (see all 20)    About this scheme

    ExUns: 1 ^ 2a · 2b · 2c · 2d · 2e ^ 3a · 3b ^ 4 ^ 5a · 5b · 5c ^ 6 ^ 7a · 7b · 7c ^ 8 ^ 9 ^ 10a · 10b · 10c ^ 11 ^ 12 ^ 13a · 13b · 13c ^
    SP1:                                      -     -     -                 -     -     -     -     -     -                 -     -                 -               
    SP2:                                      -     -     -                 -     -     -     -     -     -                                         -               
    SP3:                                                                                                                                            -               
    SP4:        -     -     -     -     -     -     -     -                 -     -     -     -     -     -                                         -               
    SP5:                                      -     -     -                 -     -     -     -     -     -     -     -     -     -     -     -     -               

    ExUns: 14 ^ 15a · 15b · 15c · 15d ^ 16a · 16b ^ 17a · 17b ^ 18
    SP1:                          -                                 
    SP2:                          -                                 
    SP3:                          -                                 
    SP4:                                                            
    SP5:                          -                                 


    ECgene alternative splicing isoforms for SPHK2

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    SPHK2 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: CGCGTCCTCC
    SPHK2 Expression
    About this image


    SPHK2 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 5) fully expand
     
     Primitive Streak (Early Embryo)
             Extraembryonic Mural Cells Extraembryonic Mesoderm
     
     Endothelium (Cardiovascular System)
             Angioblasts Embryonic Blood Islands
     
     Extraembryonic Mesoderm (Extraembryonic Tissues)
             Extraembryonic Mural Cells Extraembryonic Mesoderm
     
     Epiblast (Early Embryo)
             Extraembryonic Mural Cells Extraembryonic Mesoderm
     
     Lateral Plate Mesoderm (Gastrulation Derivatives)
             Angioblasts Embryonic Blood Islands
    SPHK2 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    SPHK2 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.528006
        Pathway & Disease-focused RT2 Profiler PCR Arrays including SPHK2: 
              Hypertension in human mouse rat
              VEGF Signaling in human mouse rat
              Apoptosis in human mouse rat

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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of animals and fungi.

    Orthologs for SPHK2 gene from Selected species (see all 13)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Sphk21 , 5 sphingosine kinase 21, 5 80.92(n)1
    84.03(a)1
      7 (29.45 cM)5
    566321  NM_020011.51  NP_064395.21 
     457094675 
    lizard
    (Anolis carolinensis)
    Reptilia SPHK26
    sphingosine kinase 2
    55(a)
    1 ↔ 1
    GL343279.1(921436-941183)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia sphk21 sphingosine kinase 2 57.41(n)
    57.35(a)
      100494664  XM_002938279.2  XP_002938325.1 
    zebrafish
    (Danio rerio)
    Actinopterygii Dr.129672 Transcribed sequence with weak similarity to protein more 75.96(n)    57097328 
    fruit fly
    (Drosophila melanogaster)
    Insecta Sk11 , 3 phosphorylation diacylglycerol kinase3
    Sphingosine kinase 11
    37(a)3
    49.18(n)1
    41.63(a)1
      10B133
    320891  NM_132489.21  NP_572717.11 
    worm
    (Caenorhabditis elegans)
    Secernentea sphk-11 sphk-1 40.16(n)
    32.08(a)
      183197  NM_001026847.4  NP_001022018.1 
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes LCB46
    LCB56
    Sphingoid long-chain base kinase, responsible for ...
    Minor sphingoid long-chain base kinase, paralog of...
    19(a)
    18(a)
    many ↔ many
    many ↔ many
    XV(652010-653884) YOR171C
    XII(665844-667907) YLR260W


    ENSEMBL Gene Tree for SPHK2 (if available)
    TreeFam Gene Tree for SPHK2 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for SPHK2 gene
    CERKL2  SPHK12  CERK2  AGK2  
    2 SIMAP similar genes for SPHK2 using alignment to 7 protein entries:     SPHK2_HUMAN (see all proteins):
    SPHK-2    SPHK1

    SPHK2 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for SPHK2 (see all 364)    About this table    
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 19 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1113432571,2
    C,F--49124965(+) ATTTCG/ATGGGG 4 -- int12Minor allele frequency- A:0.05CSA WA 120
    rs784717231,2
    C,F--49125065(+) ACTTTT/ACAAGG 4 -- int11Minor allele frequency- A:0.50WA 2
    rs1128181011,2
    F--49126236(+) GTGGCC/TCACGC 5 -- int1 us2k11Minor allele frequency- T:0.50CSA 2
    rs1112521481,2
    C,F--49126251(+) AATCCC/TAGCAC 5 -- int1 us2k11Minor allele frequency- T:0.50CSA 2
    rs2013931571,2
    C--49296855(+) GAAAGG/TGACAG 3 -- us2k11Minor allele frequency- T:0.00EU 1321
    rs1469796051,2
    F--49296858(+) AGGGAC/AAGAGG 3 -- us2k11Minor allele frequency- A:0.00NA 4552
    rs1132016911,2
    C--49296953(+) TAACTG/TGAGGG 3 -- us2k10--------
    rs2005232761,2
    C--49296954(+) AACTGG/AAGGGA 3 -- us2k11Minor allele frequency- A:0.00EU 1309
    rs2007468851,2
    C--49296956(+) CTGGAG/CGGACG 3 -- us2k11Minor allele frequency- C:0.00EU 1305
    rs2020102021,2
    --49296957(+) TGGAGA/GGACGG 3 -- us2k10--------

    HapMap Linkage Disequilibrium report for SPHK2 (49122548 - 49133974 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 5 variations for SPHK2:    About this table    
    Variant IDTypeSubtypePubMed ID
    dgv3967n71CNV Loss21882294
    nsv912225CNV Loss21882294
    dgv3966n71CNV Loss21882294
    esv1003432CNV Gain20482838
    nsv9739CNV Gain+Loss18304495

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing SPHK2
    DNA2.0 Custom Variant and Variant Library Synthesis for SPHK2

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 607092    OMIM disorders: --

    Selected diseases for SPHK2 (see all 23):    
    About MalaCards
    lymphopenia    keloids    breast cancer    hodgkin's lymphoma
    retinal degeneration    non-hodgkin lymphoma    retinitis    hypoxia
    tuberculosis    multiple sclerosis    hepatitis b    hypertension
    bipolar disorder    inflammatory bowel disease    rheumatoid arthritis    arthritis
    malaria    lung cancer    endotheliitis    hepatitis

    1 disease from the University of Copenhagen DISEASES database for SPHK2:
    Lymphopenia

    SPHK2 for disorders           About GeneDecksing

    1 Novoseek inferred disease relationship for SPHK2 gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    breast cancer 19.1 4 15951439 (1), 17311928 (1), 20110355 (1)

    Human Genome Epidemiology (HuGE) Navigator: SPHK2 (1 document)

    Export disorders for SPHK2 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for SPHK2 gene, integrated from 10 sources (see all 75):
    (articles sorted by number of sources associating them with SPHK2)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Molecular cloning and functional characterization of a novel mammalian sphingosine kinase type 2 isoform. (PubMed id 10751414)1, 2, 3, 9 Liu H.... Spiegel S. (J. Biol. Chem. 2000)
    2. A lipid binding domain in sphingosine kinase 2. (PubMed id 19168031)1, 2, 9 Don A.S. and Rosen H. (Biochem. Biophys. Res. Commun. 2009)
    3. Integral and associated lysosomal membrane proteins. (PubMed id 17897319)1, 2 Schroeder B.... Hasilik A. (Traffic 2007)
    4. Recycling of sphingosine is regulated by the concerted actions of sphingosine-1-phosphate phosphohydrolase 1 and sphingosine kinase 2. (PubMed id 17895250)1, 3 Le Stunff H....Spiegel S. (J. Biol. Chem. 2007)
    5. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (Nat. Genet. 2004)
    6. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    7. Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs. (PubMed id 11230166)1, 2 Wiemann S.... Poustka A. (Genome Res. 2001)
    8. Role of sphingosine kinase 2 in cell migration toward epidermal growth factor. (PubMed id 15951439)1, 9 Hait N.C....Spiegel S. (J. Biol. Chem. 2005)
    9. Expression of sphingosine kinase 2 in synovial fibroblasts of rheumatoid arthritis contributing to apoptosis by a sphingosine analogue, FTY720. (PubMed id 19490468)1, 9 Kamada K....Nose M. (Pathol. Int. 2009)
    10. Protein kinase D-mediated phosphorylation and nuclear export of sphingosine kinase 2. (PubMed id 17635916)1, 9 Ding G....Nakamura S. (J. Biol. Chem. 2007)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 56848 HGNC: 18859 AceView: SPHK2 Ensembl:ENSG00000063176 euGenes: HUgn56848
    ECgene: SPHK2 Kegg: 56848 H-InvDB: SPHK2

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for SPHK2 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for SPHK2 gene:
    Search GeneIP for patents involving SPHK2

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, and ESI BIO, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
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