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Aliases for SPHK1 Gene

Aliases for SPHK1 Gene

  • Sphingosine Kinase 1 2 3
  • SPK 1 3 4
  • SK 1 3 4
  • SPHK 3 4
  • EC 2.7.1.91 4
  • SPK 4

External Ids for SPHK1 Gene

Previous GeneCards Identifiers for SPHK1 Gene

  • GC17U990140
  • GC17P077371
  • GC17P074843
  • GC17P074977
  • GC17P071890
  • GC17P074380
  • GC17P069809

Summaries for SPHK1 Gene

Entrez Gene Summary for SPHK1 Gene

  • The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

GeneCards Summary for SPHK1 Gene

SPHK1 (Sphingosine Kinase 1) is a Protein Coding gene. Diseases associated with SPHK1 include glioblastoma and breast cancer. Among its related pathways are PDGFR-beta signaling pathway and Human Embryonic Stem Cell Pluripotency. GO annotations related to this gene include magnesium ion binding and NAD+ kinase activity. An important paralog of this gene is SPHK2.

UniProtKB/Swiss-Prot for SPHK1 Gene

  • Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.

Tocris Summary for SPHK1 Gene

  • Sphingosine kinase (SphK) mediates the conversion of sphingosine to sphingosine-1-phosphate, a key sphingolipid signaling molecule involved in cell growth, survival, differentiation and motility. SphK constitutes an important checkpoint in the regulation of circulating levels of ceramide, sphingosine and sphingosine-1-phosphate. Two mammalian isoforms have been cloned - SPHK1 and SPHK2

Gene Wiki entry for SPHK1 Gene

No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for SPHK1 Gene

Genomics for SPHK1 Gene

Regulatory Elements for SPHK1 Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for SPHK1 Gene

Start:
76,376,584 bp from pter
End:
76,387,860 bp from pter
Size:
11,277 bases
Orientation:
Plus strand

Genomic View for SPHK1 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for SPHK1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for SPHK1 Gene

Proteins for SPHK1 Gene

  • Protein details for SPHK1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9NYA1-SPHK1_HUMAN
    Recommended name:
    Sphingosine kinase 1
    Protein Accession:
    Q9NYA1
    Secondary Accessions:
    • Q8N632
    • Q96GK1
    • Q9HD92
    • Q9NY70
    • Q9NYL3

    Protein attributes for SPHK1 Gene

    Size:
    384 amino acids
    Molecular mass:
    42518 Da
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    • Interacts with ACY1 (By similarity). Binds to calmodulin. Interacts with SPHKAP. Interacts with CIB1, the interaction occurs in a calcium-dependent manner.
    Miscellaneous:
    • Sphingosine 1-phosphate stimulates TRAF2 E3 ubiquitin ligase activity, and promotes activation of NF-kappa-B in response to TNF signaling

    Three dimensional structures from OCA and Proteopedia for SPHK1 Gene

    Alternative splice isoforms for SPHK1 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for SPHK1 Gene

Proteomics data for SPHK1 Gene at MOPED

Post-translational modifications for SPHK1 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Antibody Products

  • R&D Systems Antibodies for SPHK1 (Sphingosine Kinase 1/SPHK1)
  • Cell Signaling Technology (CST) Antibodies for SPHK1 (SPHK1)

Protein Products

No data available for DME Specific Peptides for SPHK1 Gene

Domains for SPHK1 Gene

Gene Families for SPHK1 Gene

Protein Domains for SPHK1 Gene

Suggested Antigen Peptide Sequences for SPHK1 Gene

Graphical View of Domain Structure for InterPro Entry

Q9NYA1

UniProtKB/Swiss-Prot:

SPHK1_HUMAN :
  • Q9NYA1
Domain:
  • Contains 1 DAGKc domain.
genes like me logo Genes that share domains with SPHK1: view

Function for SPHK1 Gene

Molecular function for SPHK1 Gene

UniProtKB/Swiss-Prot BiophysicochemicalProperties: Kinetic parameters: KM=14 uM for sphingosine {ECO:0000269 PubMed:10947957}; KM=20 uM for dihydrosphingosine {ECO:0000269 PubMed:10947957}; KM=77 uM for ATP {ECO:0000269 PubMed:10947957}; pH dependence: Optimum pH is 7.4. {ECO:0000269 PubMed:10947957};
UniProtKB/Swiss-Prot CatalyticActivity: ATP + sphinganine = ADP + sphinganine 1-phosphate
UniProtKB/Swiss-Prot CatalyticActivity: ATP + sphingosine = ADP + sphingosine 1-phosphate
UniProtKB/Swiss-Prot Function: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.

Enzyme Numbers (IUBMB) for SPHK1 Gene

Gene Ontology (GO) - Molecular Function for SPHK1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000287 magnesium ion binding ISS --
GO:0003677 DNA binding IDA 12393916
GO:0003951 NAD+ kinase activity IEA --
GO:0004143 diacylglycerol kinase activity IEA --
GO:0005515 protein binding IPI 12080051
genes like me logo Genes that share ontologies with SPHK1: view
genes like me logo Genes that share phenotypes with SPHK1: view

Animal Models for SPHK1 Gene

MGI Knock Outs for SPHK1:

Animal Model Products

CRISPR Products

miRNA for SPHK1 Gene

miRTarBase miRNAs that target SPHK1

No data available for Transcription Factor Targeting and HOMER Transcription for SPHK1 Gene

Localization for SPHK1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for SPHK1 Gene

Cytoplasm. Nucleus. Cell membrane. Note=Translocated from the cytoplasm to the plasma membrane in a CIB1-dependent manner.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for SPHK1 Gene COMPARTMENTS Subcellular localization image for SPHK1 gene
Compartment Confidence
cytosol 5
nucleus 5
plasma membrane 4
extracellular 2
mitochondrion 2
cytoskeleton 1
endoplasmic reticulum 1
peroxisome 1
vacuole 1

Gene Ontology (GO) - Cellular Components for SPHK1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IEA --
GO:0005737 cytoplasm IDA --
GO:0005829 cytosol TAS 10863092
GO:0005886 plasma membrane IDA --
GO:0008021 synaptic vesicle IEA --
genes like me logo Genes that share ontologies with SPHK1: view

Pathways for SPHK1 Gene

genes like me logo Genes that share pathways with SPHK1: view

PCR Array Products

Gene Ontology (GO) - Biological Process for SPHK1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001568 blood vessel development IEA --
GO:0001934 positive regulation of protein phosphorylation --
GO:0001956 positive regulation of neurotransmitter secretion IEA --
GO:0003376 sphingosine-1-phosphate signaling pathway IMP 23106337
GO:0006457 protein folding TAS --
genes like me logo Genes that share ontologies with SPHK1: view

Compounds for SPHK1 Gene

(2) Tocris Compounds for SPHK1 Gene

Compound Action Cas Number
N,N-Dimethylsphingosine Sphingosine kinase inhibitor [119567-63-4]
SK1-I Sphingosine kinase 1 (SphK1) inhibitor; antiproliferative [1072443-89-0]

(42) HMDB Compounds for SPHK1 Gene

Compound Synonyms Cas Number PubMed IDs
Adenosine triphosphate
  • 5'-(tetrahydrogen triphosphate) Adenosine
56-65-5
ADP
  • adenosindiphosphorsaeure
58-64-0
Cer(d18:0/12:0)
Not Available
Cer(d18:0/14:0)
Not Available
Cer(d18:0/16:0)
Not Available

(25) Novoseek inferred chemical compound relationships for SPHK1 Gene

Compound -log(P) Hits PubMed IDs
sphingosine 97.7 199
n,n-dimethylsphingosine 93.7 16
ceramide 83.9 41
sphinganine 1-phosphate 80.4 3
ceramides 55.1 1
genes like me logo Genes that share compounds with SPHK1: view

Transcripts for SPHK1 Gene

Unigene Clusters for SPHK1 Gene

Sphingosine kinase 1:
Representative Sequences:

CRISPR Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for SPHK1

Primer Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for SPHK1 Gene

ExUns: 1 ^ 2 ^ 3a · 3b ^ 4 ^ 5a · 5b · 5c · 5d ^ 6a · 6b · 6c ^ 7a · 7b · 7c · 7d ^ 8 ^ 9a · 9b · 9c · 9d ^ 10a · 10b
SP1: - - - - - - - - - - - -
SP2: - - - - -
SP3: - - - -
SP4: - - - -
SP5: - - - - - - - - - -
SP6: - - - - - - -
SP7: - - - - - - -
SP8: - - -
SP9: - - - - - - - -
SP10: - -
SP11:

Relevant External Links for SPHK1 Gene

GeneLoc Exon Structure for
SPHK1
ECgene alternative splicing isoforms for
SPHK1

Expression for SPHK1 Gene

mRNA expression in normal human tissues for SPHK1 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for SPHK1 Gene

SOURCE GeneReport for Unigene cluster for SPHK1 Gene Hs.68061

mRNA Expression by UniProt/SwissProt for SPHK1 Gene

Q9NYA1-SPHK1_HUMAN
Tissue specificity: Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle.
genes like me logo Genes that share expressions with SPHK1: view

In Situ Assay Products

No data available for mRNA differential expression in normal tissues for SPHK1 Gene

Orthologs for SPHK1 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for SPHK1 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia SPHK1 35
  • 99.3 (n)
  • 99.74 (a)
SPHK1 36
  • 99 (a)
OneToOne
cow
(Bos Taurus)
Mammalia SPHK1 35
  • 82.53 (n)
  • 80.57 (a)
SPHK1 36
  • 79 (a)
OneToOne
dog
(Canis familiaris)
Mammalia SPHK1 35
  • 86.01 (n)
  • 88.92 (a)
SPHK1 36
  • 89 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Sphk1 35
  • 80.34 (n)
  • 82.61 (a)
Sphk1 16
Sphk1 36
  • 79 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia SPHK1 36
  • 74 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia -- 36
  • 90 (a)
OneToMany
-- 36
  • 66 (a)
OneToMany
rat
(Rattus norvegicus)
Mammalia Sphk1 35
  • 78.66 (n)
  • 77.29 (a)
chicken
(Gallus gallus)
Aves SPHK1 35
  • 65.35 (n)
  • 58.31 (a)
SPHK1 36
  • 54 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia SPHK1 36
  • 40 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia sphk1 35
  • 59.65 (n)
  • 55.4 (a)
zebrafish
(Danio rerio)
Actinopterygii LOC100331840 35
  • 61.38 (n)
  • 55.39 (a)
SPHK1 36
  • 54 (a)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP006995 35
  • 52.87 (n)
  • 40.48 (a)
fruit fly
(Drosophila melanogaster)
Insecta Sk1 36
  • 22 (a)
ManyToMany
Sk2 35
  • 51.63 (n)
  • 39.78 (a)
Sk2 36
  • 21 (a)
ManyToMany
Sk1 37
  • 42 (a)
worm
(Caenorhabditis elegans)
Secernentea sphk-1 36
  • 23 (a)
OneToMany
C34C6.5 37
  • 28 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes LCB4 36
  • 16 (a)
ManyToMany
LCB5 36
  • 15 (a)
ManyToMany
LCB5 38
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 29 (a)
OneToMany
Species with no ortholog for SPHK1:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for SPHK1 Gene

ENSEMBL:
Gene Tree for SPHK1 (if available)
TreeFam:
Gene Tree for SPHK1 (if available)

Paralogs for SPHK1 Gene

Paralogs for SPHK1 Gene

Selected SIMAP similar genes for SPHK1 Gene using alignment to 5 proteins:

genes like me logo Genes that share paralogs with SPHK1: view

Variants for SPHK1 Gene

Sequence variations from dbSNP and Humsavar for SPHK1 Gene

SNP ID Clin Chr 17 pos Sequence Context AA Info Type MAF
rs191520 -- 76,388,326(-) aactt(C/T)tgggc downstream-variant-500B
rs346797 -- 76,382,820(+) CCAGT(A/C)CCCTC upstream-variant-2KB
rs346798 -- 76,382,161(+) CCCCC(A/C/G)ACCCG upstream-variant-2KB
rs346799 -- 76,388,042(-) AGAGG(C/T)CCCCC downstream-variant-500B
rs346800 -- 76,387,991(-) CAGAA(C/T)GACTA downstream-variant-500B

Structural Variations from Database of Genomic Variants (DGV) for SPHK1 Gene

Variant ID Type Subtype PubMed ID
nsv833547 CNV Loss 17160897
nsv2145 CNV Loss 18451855
nsv833549 CNV Gain 17160897
esv23649 CNV Loss 19812545

Relevant External Links for SPHK1 Gene

HapMap Linkage Disequilibrium report
SPHK1

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for SPHK1 Gene

Disorders for SPHK1 Gene

MalaCards: The human disease database

MalaCards: The human disease database. (2) Diseases for SPHK1 Gene including...

Search for SPHK1 Gene in MalaCards »

(1) University of Copenhagen DISEASES for SPHK1 Gene

(17) Novoseek inferred disease relationships for SPHK1 Gene

Disease -log(P) Hits PubMed IDs
glioblastoma 44.4 12
glioblastoma multiforme 35.6 4
renal fibrosis 27 2
cancer 25.9 23
tumors 22.4 16

Relevant External Links for SPHK1

Human Genome Epidemiology (HuGE) Navigator
SPHK1
genes like me logo Genes that share disorders with SPHK1: view

No data available for UniProtKB/Swiss-Prot for SPHK1 Gene

Publications for SPHK1 Gene

  1. Identification of functional nuclear export sequences in human sphingosine kinase 1. (PMID: 14575709) Inagaki Y. … Igarashi Y. (Biochem. Biophys. Res. Commun. 2003) 3 4 23
  2. Translocation of sphingosine kinase 1 to the plasma membrane is mediated by calcium- and integrin-binding protein 1. (PMID: 19854831) Jarman K.E. … Pitson S.M. (J. Biol. Chem. 2010) 3 4 23
  3. Sphingosine kinase-1 is central to androgen-regulated prostate cancer growth and survival. (PMID: 19956567) Dayon A. … Cuvillier O. (PLoS ONE 2009) 3 23
  4. Isoflurane via TGF-beta1 release increases caveolae formation and organizes sphingosine kinase signaling in renal proximal tubules. (PMID: 20053797) Song J.H. … Lee H.T. (Am. J. Physiol. Renal Physiol. 2010) 3 23
  5. Molecular cloning and functional characterization of murine sphingosine kinase. (PMID: 9726979) Kohama T. … Spiegel S. (J. Biol. Chem. 1998) 2 3

Products for SPHK1 Gene

  • Addgene plasmids for SPHK1

Sources for SPHK1 Gene

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