SPC24 Gene
protein-coding GIFtS : 48
GCID: GC19 M011257
SPC24, NDC80 kinetochore complex component, homolog (S.... (Previous name: spindle pole body component 24 homolog (S. cerevisiae)... ) (Previous symbol: SPBC24 )
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Aliasesfor SPC24 gene
(According to
1 HGNC ,
2 Entrez Gene ,
3 UniProtKB/Swiss-Prot ,
4 UniProtKB/TrEMBL , 5 OMIM , 6 GeneLoc ,
7 Ensembl ,
8 DME ,
9 miRBase ,
and/or 10 fRNAdb )About This Section Aliases SPC24, NDC80 Kinetochore Complex Component, Homolog (S. Cerevisiae) 1 2 Spindle Pole Body Component 24 Homolog (S. Cerevisiae)1 SPBC241 2 3 Kinetochore Protein Spc242 HSpc241 Spindle Pole Body Component 24 Homolog2 FLJ908061
Export aliases for SPC24 gene to outside databases Previous GC identifers: GC19M011119 GC19M010834
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Summariesfor SPC24 gene (According to Entrez Gene ,
Tocris Bioscience ,
Wikipedia's
Gene Wiki ,
PharmGKB ,
UniProtKB/Swiss-Prot ,
and/or
UniProtKB/TrEMBL )
About This Section UniProtKB/Swiss-Prot: SPC24_HUMAN, Q8NBT2 Function : Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosomesegregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore Gene Wiki entry for SPC24
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Genomic Viewsfor SPC24 gene
(According to
GeneLoc and/or
HGNC , and/or
Entrez Gene (NCBI build 37) ,
and/or miRBase ,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69) ,
Regulatory elements and Epigenetics data according to
QIAGEN ,
SABiosciences , and/or
SwitchGear Genomics )About This Section RefSeq DNA sequence: NC_000019.9 NC_018930.1 NT_011295.11 Regulatory elements: SABiosciences Regulatory transcription factor binding sites in the SPC24 gene promoter: ER-alpha GR p300 Pax-6 GR-beta MIF-1 XBP-1 Arnt SEF-1 (1) GR-alpha Other transcription factors Search SABiosciences Chromatin IP Primers for SPC24 Epigenetics: QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human , mouse , rat SPC24
Genomic Location: Genomic View : UCSC Golden Path with GeneCards custom track Entrez Gene cytogenetic band: 19p13.2 Ensembl cytogenetic band: 19p13.2 HGNC cytogenetic band: 19p13.2 SPC24 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different) GeneLoc information about chromosome 19 GeneLoc Exon Structure
GeneLoc location for GC19M011257: view genomic region
(about GC identifiers )
Start:
11,242,196 bp from pter
End:
11,266,484 bp from pter
Size:
24,289 bases
Orientation:
minus strand
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Proteinsfor SPC24 gene
(According to
1 UniProtKB ,
HORDE ,
neXtProt ,
Ensembl ,
and/or Reactome ,
Modification sites according to 2 PhosphoSitePlus ,
Specific Peptides from DME ,
Protein expression images according to data from SPIRE MOPED and PaxDb ,
RefSeq according to NCBI ,
PDB rendering according to OCA and/or Proteopedia ,
Recombinant Proteins
from
EMD Millipore ,
R&D Systems ,
GenScript ,
Enzo Life Sciences ,
OriGene ,
Novus Biologicals ,
Sino Biological ,
ProSpec , and/or
Uscn ,
Biochemical Assays by
EMD Millipore ,
R&D Systems ,
OriGene ,
GenScript ,
Cell Signaling Technology ,
Enzo Life Sciences , and/or
Uscn ,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene ,
Antibodies by
EMD Millipore ,
R&D Systems ,
GenScript ,
Cell Signaling Technology ,
OriGene ,
Novus Biologicals ,
Thermo Fisher Scientific ,
Abcam , and/or
Uscn )
About This Section UniProtKB/Swiss-Prot: SPC24_HUMAN, Q8NBT2 (See
protein sequence )Recommended Name: Kinetochore protein Spc24 Size : 197 amino acids; 22478 Da
Subunit : Component of the NDC80 complex, which consists of NDC80/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex isformed by two subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24-SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C-terminal regions of both subunits of the NDC80/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end
Subcellular location : Nucleus. Chromosome, centromere, kinetochore. Note=Localizes to kinetochores from late prophaseto anaphase. Localizes specifically to the outer plate of the kinetochore
2 PDB 3D structures from and Proteopedia for SPC24 :2VE7 (3D)
  3IZ0 (3D)
 
Secondary accessions : C9JGC4Explore the universe of human proteins at neXtProt for SPC24: NX_Q8NBT2 Post-translational modifications:
View modification sites using PhosphoSitePlus 2 View neXtProt modification sites for NX_Q8NBT2 SPC24 Protein expression data from MOPED and PaxDb : About this image
REFSEQ proteins: NP_872319.1 ENSEMBL proteins: ENSP00000468818 ENSP00000465075 ENSP00000466688 ENSP00000465335 ENSP00000397131 ENSP00000468366 ENSP00000465167 ENSP00000412944 Reactome Protein details: Q8NBT2 Human Recombinant Protein Products: Gene Ontology (GO): 4 cellular component terms (GO ID links to tree view) : About this table
SPC24 for ontologies About GeneDecksing SPC24 Antibody Products: Assay Products for SPC24:
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Protein
Domains / Familiesfor SPC24 gene (According to InterPro , ProtoNet ,
UniProtKB , and/or BLOCKS ,
Sets of similar genes according to GeneDecks )
About This Section
SPC24 for domains About GeneDecksing 1 InterPro domain/family :
Graphical View of Domain Structure for InterPro Entry Q8NBT2 ProtoNet protein and cluster: Q8NBT2
UniProtKB/Swiss-Prot: SPC24_HUMAN, Q8NBT2 Similarity : Belongs to the SPC24 family
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Functionfor SPC24 gene
(According to 1 UniProtKB ,
Genatlas ,
LifeMap Discovery™ ,
IUBMB , and/or
2 DME ,
Human phenotypes from GenomeRNAi ,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences ,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene ,
RNAi from
EMD Millipore ,
siRNAs from
OriGene ,
QIAGEN ,
microRNA from QIAGEN ,
Gene Editing from DNA2.0 ,
Clones from EMD Millipore ,
OriGene ,
SwitchGear Genomics ,
GenScript ,
Sino Biological ,
DNA2.0 ,
and Vector BioLabs ,
Cell Lines from GenScript ,
LifeMap BioReagents ,
In Situ Hybridization Assays from Advanced Cell Diagnostics ,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene .)
About This Section Function Summary: UniProtKB/Swiss-Prot: SPC24_HUMAN, Q8NBT2 Function : Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosomesegregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore
Clone Products: Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human , mouse , rat for SPC24 (see all 2 ) OriGene untagged cDNA clones in CMV expression vector in human , mouse , rat for SPC24OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling GenScript: all cDNA clones in your preferred vector: SPC24 (NM_182513 ) Browse Sino Biological Human cDNA Clones DNA2.0 Custom Codon Optimized Gene
Synthesis Service for SPC24 Vector BioLabs ready-to-use adenovirus/AAV for human , mouse , rat SPC24
Gene Ontology (GO): 1 molecular function term (GO ID links to tree view) : About this table
SPC24 for ontologies About GeneDecksing 1 GenomeRNAi human phenotype for SPC24 :
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Pathways & Interactionsfor SPC24 gene
(Pathways according to
EMD Millipore ,
R&D Systems ,
Cell Signaling Technology ,
KEGG ,
PharmGKB ,
BioSystems ,
Reactome ,
Tocris Bioscience ,
GeneGo (Thomson Reuters) ,
QIAGEN ,
and/or UniProtKB ,
Sets of similar genes according to GeneDecks ,
Interaction Networks according to
SABiosciences ,
and/or STRING ,
Interactions according to 1 UniProtKB ,
2 MINT ,
3 I2D , and/or
4 STRING ,
with links to IntAct and
Ensembl ,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene) .
About This Section Unified GeneCards pathways   About this table See pathways by source Super-pathway contained gene-specific pathways 1 M Phase 2 Resolution of Sister Chromatid Cohesion 3 Cell Cycle 4 PLK1 signaling events
Pathway sources See GeneCards unified pathways Show all pathways 1 BioSystems Pathway for SPC24 5/10
Reactome Pathways for SPC24 (see all 10 )
SPC24 for pathways About GeneDecksing Interactions: SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for SPC24 STRING Interaction
Network Preview (showing 5 interactants - click image to see 25)5/71 Interacting proteins for SPC24 (Q8NBT2 1 , 3 ENSP00000397131 4 ) via UniProtKB, MINT, STRING , and/or I2D (see all 71 )About this table Gene Ontology (GO): 4 biological process terms (GO ID links to tree view) : About this table
SPC24 for ontologies About GeneDecksing
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Drugs & Compoundsfor SPC24 gene (Chemical Compounds according to UniProtKB , Enzo Life Sciences ,
EMD Millipore , Tocris Bioscience
HMDB ,
BitterDB , and/or
Novoseek , and Drugs according to
DrugBank ,
Enzo Life Sciences , and/or
PharmGKB , with drugs/clinical trials/news
search links to CenterWatch )
About This Section Browse Tocris compounds for SPC24 Search CenterWatch for drugs/clinical trials and news about SPC24
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Transcriptsfor SPC24 gene (Secondary structures according to
fRNAdb ,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank , RefSeq according to Entrez Gene ,
DOTS (version 10), and/or
AceView ,
transcript ids from Ensembl
with links to UCSC ,
exon structure from GeneLoc ,
alternative splicing isoforms according to ASD and/or
ECgene ,
RNAi Products from
EMD Millipore ,
siRNAs from
OriGene ,
QIAGEN ,
shRNA from
OriGene ,
microRNA from QIAGEN ,
Tagged/untagged cDNA clones from
OriGene ,
SwitchGear Genomics ,
GenScript ,
DNA2.0 ,
Vector BioLabs ,
Primers from
OriGene ,
SABiosciences , and/or
QIAGEN
)About This Section REFSEQ mRNAs for SPC24 gene: NM_182513.2 Unigene Cluster for SPC24:
SPC24, NDC80 kinetochore complex component, homolog (S. cerevisiae) Hs.381225 [show with all ESTs ] Unigene Representative Sequence: AK075287 8 Ensembl transcripts including schematic representations, and UCSC links where relevant : ENST00000585567 ENST00000592540 ENST00000591396 ENST00000592967 ENST00000423327 (uc002mql.2 )ENST00000586708 ENST00000585486 ENST00000429831 Clone Products: OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human , mouse , rat for SPC24 (see all 2 ) OriGene untagged cDNA clones in CMV expression vector in human , mouse , rat for SPC24OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling GenScript: all cDNA clones in your preferred vector: SPC24 (NM_182513 ) DNA2.0 Custom Codon Optimized Gene
Synthesis Service for SPC24 Vector BioLabs ready-to-use adenovirus/AAV for human , mouse , rat SPC24
Additional cDNA sequence: AK075287.1 AK303157.1 AK303266.1 AY456387.1 BC105037.1 BC105039.1
4 DOTS entries : DT.100024080 DT.40208734
DT.70100755 DT.97833674 24/50 AceView cDNA sequences (see all 50 ):
BQ063818 BQ061822 AW087430 AI564835 BQ063909 BP871131 AY456387 BQ642409 AW246001 BI029235 BG167669 BU146762 BQ650106 BU953663 AK075287 BQ929089 BG167737 W84863 BQ643295 BG201978 BX100406 BU616832 NM_182513 BQ216591 GeneLoc Exon Structure
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Expression for SPC24 gene
(RNA expression data according to
H-InvDB ,
NONCODE ,
miRBase , and
RNAdb ,
Expression images according to data from
BioGPS ,
Illumina Human BodyMap , and
CGAP
SAGE ,
Sets of similar genes according to GeneDecks ,
in vivo and in vitro expression data from LifeMap Discovery™ ,
plus additional links to
Genevestigator , and/or
SOURCE , and/or
BioGPS , and/or
UniProtKB ,
PCR Arrays from
SABiosciences ,
Primers from
OriGene ,
SABiosciences , and/or
QIAGEN ,
In Situ Hybridization Assays from Advanced Cell Diagnostics )
About This Section SPC24 expression in normal human tissues (normalized intensities) See probesets specificity/sensitivity at GeneAnnot About this image BioGPS CGAP TAG: GAGAAGGAAG
About this image SPC24 expression in embryonic tissues and stem cells Expression by the Database of Embryonic development, Stem cell research, and
Regenerative medicine About this table See SPC24 Protein Expression from SPIRE MOPED and PaxDB Genevestigator expression for SPC24 SOURCE GeneReport for Unigene cluster: Hs.381225 SABiosciences Custom PCR Arrays for SPC24 Primer Products: OriGene genome-wide validated SYBR primer pairs in human , mouse , rat for SPC24Browse OriGene validated miRNA SYBR primer pairs SABiosciences RT2 qPCR Primer Assay in human , mouse , rat SPC24 QIAGEN QuantiTect SYBR Green Assays in human , mouse , rat SPC24 QIAGEN QuantiFast Probe-based Assays in human , mouse , rat SPC24
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Orthologsfor SPC24 gene
(Orthologs according to
1,2 HomoloGene (2 older version, for species not in 1 newer version),
3 euGenes ,
4 SGD
,
5 MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase ,
and/or
6 Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam )
About This Section
This gene was present in the common ancestor of chordates.
Orthologs for SPC24 gene from 2/7 species (see all 7 ) About this table
ENSEMBL Gene Tree for SPC24 (if available)TreeFam Gene Tree for SPC24 (if available)
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Paralogsfor SPC24 gene (Paralogs according to
1 HomoloGene , 2 Ensembl , and 3 SIMAP , Pseudogenes according to 4 Pseudogene.org Build 68)About This Section --
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Genomic Variantsfor SPC24 gene (SNPs/Variants according to the
1 NCBI SNP Database ,
2 Ensembl ,
3 PupaSUITE ,
UniProtKB , and
DNA2.0 ,
Linkage Disequilibrium by HapMap ,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants , Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB) , Blood group antigen gene mutations by BGMUT ,
Resequencing Primers from QIAGEN ,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences )
About This Section
Genomic Data Transcription Related Data Allele Frequencies SNP ID Valid Clinical significance Chr 19 pos Sequence # AA Chg Type More # Allele freq Pop Total sample More
HapMap Linkage Disequilibrium report for SPC24 (11242196 - 11266484 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions) Database of Genomic Variants (DGV) variations for SPC24: --
SABiosciences Cancer Mutation PCR Assays
QIAGEN SeqTarget long-range PCR primers in human , mouse , rat for resequencing SPC24
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Disorders
/ Diseasesfor SPC24 gene
(in which this Gene is Involved, According to MalaCards ,
OMIM, UniProtKB ,
the University of Copenhagen DISEASES
database ,
Genatlas , GeneTests ,
GAD ,
HuGE Navigator ,
and/or TGDB .)
About This Section
SPC24 for disorders About MalaCards
SPC24 for disorders About GeneDecksing
OMIM gene information: 609394
OMIM disorders : --Export disorders for SPC24 gene to outside databases
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Publicationsfor SPC24 gene (in
PubMed .
Associations of this gene to articles via
1 Entrez Gene ,
2 UniProtKB/Swiss-Prot ,
3 HGNC ,
4 GAD ,
5 PharmGKB ,
6 HMDB ,
7 DrugBank ,
8 UniProtKB/TrEMBL ,
9 Novoseek , and/or
10 fRNAdb )
About This Section PubMed articles for SPC24 gene, integrated from 9 sources (see all 23 ): (articles sorted by number of sources associating them with SPC24) Utopia : connect your pdf to the dynamic world of online information
Polo-like kinase 1 creates the tension-sensing 3F3/2 phosphoepitope and modulates the association of spindle-checkpoint proteins at kinetochores. (PubMed id 15964272) 1 , 2 , 9 Ahonen L.J.... Gorbsky G.J. (2005) Architecture of the human Ndc80-Hec1 complex, a critical constituent of the outer kinetochore. (PubMed id 15961401) 1 , 2 Ciferri C.... Musacchio A. (2005) Identification of two novel components of the human NDC80 kinetochore complex. (PubMed id 14699129) 1 , 2 Bharadwaj R.... Yu H. (2004) The vertebrate Ndc80 complex contains Spc24 and Spc25 homologs, which are required to establish and maintain kinetochore-microtubule attachment. (PubMed id 14738735) 1 , 2 McCleland M.L.... Stukenberg P.T. (2004) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334) 1 , 2 Gerhard D.S....Malek J. (2004) Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039) 1 , 2 Ota T.... Sugano S. (2004) A census of human soluble protein complexes. (PubMed id 22939629) 1 Havugimana P.C....Emili A. (2012) Aurora B controls kinetochore-microtubule attachments by inhibiting Ska complex-KMN network interaction. (PubMed id 22371557) 1 Chan Y.W....Santamaria A. (2012) Methods for quantification of in vivo changes in prote in ubiquitination following proteasome and deubiquitinase inhibition. (PubMed id 22505724) 1 Udeshi N.D....Carr S.A. (2012) A high-throughput approach for measuring temporal chan ges in the interactome. (PubMed id 22863883) 1 Kristensen A.R....Foster L.J. (2012)
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External Searches for SPC24 gene
(in PubMed ,
OMIM , and NCBI Bookshelf ) About This Section
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Genome Databases showing SPC24 gene
(According to
Entrez Gene ,
HGNC ,
AceView ,
euGenes ,
Ensembl ,
miRBase ,
ECgene ,
Kegg ,
and/or
H-InvDB )
About This Section
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Other Databases showing SPC24 gene
(According to HUGE )
About This Section --
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Specialized Databases showing SPC24 gene (According to PharmGKB ,
ATLAS , HORDE , IMGT , LEIDEN , UniProtKB/Swiss-Prot , and/or UniProtKB/TrEMBL ,Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot )About This Section
Name Description
PharmGKB entry for SPC24 Pharmacogenomics, SNPs, Pathways
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About This Section Patent Information for SPC24 gene: Search GeneIP for patents involving SPC24 GeneCards and IP: Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
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Productsfor SPC24 gene (Antibodies, recombinant proteins, and assays from EMD Millipore , R&D Systems , OriGene , QIAGEN , GenScript , Cell Signaling Technology , SABiosciences , Novus Biologicals , Sino Biological , Enzo Life Sciences , Abcam , ProSpec , Uscn , Thermo Fisher Scientific , Gene Editing from DNA2.0 , Clones from EMD Millipore , OriGene , GenScript , Sino Biological , DNA2.0 , SwitchGear Genomics , Vector BioLabs , Cell lines from GenScript and LifeMap BioReagents , PCR Arrays from SABiosciences , Drugs and/or compounds from EMD Millipore , Tocris Bioscience , and/or
Enzo Life Sciences ), In Situ Hybridization Assays from Advanced Cell Diagnostics About This Section
Browse OriGene Antibodies OriGene shRNA RFP for SPC24 OriGene 29mer shRNA kits in GFP-retroviral vector in human , mouse , rat for SPC24 OriGene genome-wide validated SYBR primer pairs in human , mouse , rat for SPC24 OriGene Protein Over-expression Lysate for SPC24 Browse OriGene Fluorogenic Cell Assay Kits OriGene siRNA for SPC24 Browse 3'-UTR reporter clones for miRNA target validation OriGene untagged cDNA clones in CMV expression vector in human , mouse , rat for SPC24 OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human , mouse , rat for SPC24 Browse OriGene GFP tagged cDNA clones in CMV expression vector Browse OriGene MicroRNA Expression Plasmids Browse OriGene basic RS shRNAs Browse OriGene validated miRNA SYBR primer pairs OriGene Purified Protein for SPC24 OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling OriGene Custom Antibody Services for SPC24 OriGene Custom Protein Services for SPC24 OriGene Custom Immunoassay Development
QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat SPC24 QIAGEN SeqTarget long-range PCR primers in human , mouse , rat for resequencing SPC24 QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human , mouse , rat SPC24 QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human , mouse , rat SPC24 QIAGEN QuantiFast Probe-based Assays in human , mouse , rat SPC24 QIAGEN QuantiTect SYBR Green Assays in human , mouse , rat SPC24
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SPC24 Proteins, Antibodies, CLIAs, and ELISAs
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Vector BioLabs ready-to-use adenovirus/AAV for human , mouse , rat SPC24
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Aliases for SPC24
Databases for SPC24
Disorders / Diseases for SPC24
Domains / Families for SPC24
Drugs / Compounds for SPC24
Expression for SPC24
Function for SPC24
Genomic Views for SPC24
Intellectual Property for SPC24
Orthologs for SPC24
Paralogs for SPC24
Pathways / Interactions for SPC24
Products for SPC24
Proteins for SPC24
Publications for SPC24
Search Box for SPC24
Summaries for SPC24
Transcripts for SPC24
Variants for SPC24
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