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Aliases for SPAM1 Gene

Aliases for SPAM1 Gene

  • Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) 2 3
  • Hyaluronoglucosaminidase PH-20 3 4
  • Sperm Surface Protein PH-20 3 4
  • Hyal-PH20 3 4
  • HYAL3 3 4
  • PH20 3 4
  • Epididymis Secretory Sperm Binding Protein Li 96n 3
  • Sperm Adhesion Molecule 1 4
  • Hyaluronidase PH-20 3
  • EC 3.2.1.35 4
  • HEL-S-96n 3
  • SPAG15 3
  • HYAL1 3
  • HYAL5 3
  • PH-20 3
  • HYA1 3

External Ids for SPAM1 Gene

Summaries for SPAM1 Gene

Entrez Gene Summary for SPAM1 Gene

  • Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

GeneCards Summary for SPAM1 Gene

SPAM1 (Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding)) is a Protein Coding gene. Among its related pathways are Disease and Lysosome. GO annotations related to this gene include hyalurononglucosaminidase activity. An important paralog of this gene is HYAL3.

UniProtKB/Swiss-Prot for SPAM1 Gene

  • Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

Gene Wiki entry for SPAM1 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for SPAM1 Gene

Genomics for SPAM1 Gene

Genomic Location for SPAM1 Gene

Start:
123,925,232 bp from pter
End:
123,971,414 bp from pter
Size:
46,183 bases
Orientation:
Plus strand

Genomic View for SPAM1 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for SPAM1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for SPAM1 Gene

Regulatory Elements for SPAM1 Gene

Proteins for SPAM1 Gene

  • Protein details for SPAM1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P38567-HYALP_HUMAN
    Recommended name:
    Hyaluronidase PH-20
    Protein Accession:
    P38567
    Secondary Accessions:
    • Q8TC30

    Protein attributes for SPAM1 Gene

    Size:
    509 amino acids
    Molecular mass:
    57848 Da
    Quaternary structure:
    No Data Available

    Alternative splice isoforms for SPAM1 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for SPAM1 Gene

Proteomics data for SPAM1 Gene at MOPED

Post-translational modifications for SPAM1 Gene

Other Protein References for SPAM1 Gene

No data available for DME Specific Peptides for SPAM1 Gene

Domains for SPAM1 Gene

Graphical View of Domain Structure for InterPro Entry

P38567

UniProtKB/Swiss-Prot:

HYALP_HUMAN
Family:
  • Belongs to the glycosyl hydrolase 56 family.:
    • P38567
genes like me logo Genes that share domains with SPAM1: view

No data available for Gene Families for SPAM1 Gene

Function for SPAM1 Gene

Molecular function for SPAM1 Gene

GENATLAS Biochemistry: sperm adhesion molecule,possessing hyaluronidase activity,involved in sperm-egg interaction,homologous to guinea pig PH-20,paralog to HYAL1
UniProtKB/Swiss-Prot CatalyticActivity: Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate
UniProtKB/Swiss-Prot Function: Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

Enzyme Numbers (IUBMB) for SPAM1 Gene

Gene Ontology (GO) - Molecular Function for SPAM1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004415 hyalurononglucosaminidase activity IEA --
genes like me logo Genes that share ontologies with SPAM1: view

Phenotypes for SPAM1 Gene

MGI mutant phenotypes for SPAM1:
inferred from 1 alleles
genes like me logo Genes that share phenotypes with SPAM1: view

Animal Models for SPAM1 Gene

MGI Knock Outs for SPAM1:

miRNA for SPAM1 Gene

miRTarBase miRNAs that target SPAM1

No data available for Transcription Factor Targeting and HOMER Transcription for SPAM1 Gene

Localization for SPAM1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for SPAM1 Gene

Cell membrane; Lipid-anchor, GPI-anchor.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for SPAM1 Gene COMPARTMENTS Subcellular localization image for SPAM1 gene
Compartment Confidence
plasma membrane 5
extracellular 2
cytosol 1
endoplasmic reticulum 1
golgi apparatus 1
lysosome 1
mitochondrion 1
nucleus 1
peroxisome 1

Gene Ontology (GO) - Cellular Components for SPAM1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005886 plasma membrane TAS --
GO:0031225 anchored component of membrane IEA --
genes like me logo Genes that share ontologies with SPAM1: view

Pathways for SPAM1 Gene

genes like me logo Genes that share pathways with SPAM1: view

Interacting Proteins for SPAM1 Gene

STRING Interaction Network Preview (showing 2 interactants - click image to see details)
http://string-db.org/version_10/api/image/networkList?limit=0&targetmode=proteins&caller_identity=gene_cards&network_flavor=evidence&identifiers=9606.ENSP00000345849%0d%0a9606.ENSP00000264914%0d%0a9606.ENSP00000247933%0d%0a
Selected Interacting proteins: ENSP00000345849 for SPAM1 Gene via STRING

Gene Ontology (GO) - Biological Process for SPAM1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005975 carbohydrate metabolic process IEA --
GO:0007155 cell adhesion IEA --
GO:0007338 single fertilization TAS --
GO:0007339 binding of sperm to zona pellucida TAS 8195297
GO:0007342 fusion of sperm to egg plasma membrane IEA --
genes like me logo Genes that share ontologies with SPAM1: view

Compounds for SPAM1 Gene

(3) HMDB Compounds for SPAM1 Gene

Compound Synonyms Cas Number PubMed IDs
N-Acetylgalactosamine
  • 2-Acetamido-2-desoxy-b-D-talofuranose
1811-31-0
N-Acetylgalactosamine 4-sulphate
  • N-Acetyl-D-galactosamine 4-sulfate
45233-43-0
Water
  • Dihydrogen oxide
7732-18-5

(7) Novoseek inferred chemical compound relationships for SPAM1 Gene

Compound -log(P) Hits PubMed IDs
hyaluronic acid 76.4 39
glycosaminoglycan 61.7 1
biotin 23.7 1
lipid 0 1
calcium 0 2
genes like me logo Genes that share compounds with SPAM1: view

Transcripts for SPAM1 Gene

Unigene Clusters for SPAM1 Gene

Sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding):
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for SPAM1 Gene

No ASD Table

Relevant External Links for SPAM1 Gene

GeneLoc Exon Structure for
SPAM1
ECgene alternative splicing isoforms for
SPAM1

Expression for SPAM1 Gene

mRNA expression in normal human tissues for SPAM1 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for SPAM1 Gene

This gene is overexpressed in Testis (49.9).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, and MOPED for SPAM1 Gene

SOURCE GeneReport for Unigene cluster for SPAM1 Gene Hs.121494

mRNA Expression by UniProt/SwissProt for SPAM1 Gene

P38567-HYALP_HUMAN
Tissue specificity: Testis.
genes like me logo Genes that share expressions with SPAM1: view

Orthologs for SPAM1 Gene

This gene was present in the common ancestor of animals.

Orthologs for SPAM1 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia SPAM1 35
  • 99.54 (n)
  • 99.02 (a)
SPAM1 36
  • 97 (a)
OneToOne
cow
(Bos Taurus)
Mammalia -- 36
  • 56 (a)
OneToMany
-- 36
  • 63 (a)
OneToMany
LOC509761 35
  • 78.18 (n)
  • 66.52 (a)
dog
(Canis familiaris)
Mammalia SPAM1 35
  • 76.21 (n)
  • 63.68 (a)
SPAM1 36
  • 55 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Hyal5 35
  • 72.88 (n)
  • 63.6 (a)
Hyal5 36
  • 58 (a)
OneToMany
Spam1 16
Spam1 36
  • 54 (a)
OneToMany
oppossum
(Monodelphis domestica)
Mammalia SPAM1 36
  • 54 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia SPAM1 36
  • 54 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Hyal5 35
  • 71.81 (n)
  • 62.1 (a)
chicken
(Gallus gallus)
Aves SPAM1 35
  • 64.42 (n)
  • 54.48 (a)
SPAM1 36
  • 47 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia -- 36
  • 41 (a)
ManyToMany
African clawed frog
(Xenopus laevis)
Amphibia LOC398285 35
tropical clawed frog
(Silurana tropicalis)
Amphibia LOC100486024 35
  • 58.11 (n)
  • 47.81 (a)
zebrafish
(Danio rerio)
Actinopterygii spam1 35
  • 49.87 (n)
  • 43.26 (a)
spam1 36
  • 40 (a)
OneToOne
worm
(Caenorhabditis elegans)
Secernentea chhy-1 36
  • 27 (a)
OneToMany
T22C8.2 37
  • 33 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 36 (a)
OneToMany
Species with no ortholog for SPAM1:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for SPAM1 Gene

ENSEMBL:
Gene Tree for SPAM1 (if available)
TreeFam:
Gene Tree for SPAM1 (if available)

Paralogs for SPAM1 Gene

Paralogs for SPAM1 Gene

Selected SIMAP similar genes for SPAM1 Gene using alignment to 3 proteins:

genes like me logo Genes that share paralogs with SPAM1: view

Variants for SPAM1 Gene

Sequence variations from dbSNP and Humsavar for SPAM1 Gene

SNP ID Clin Chr 07 pos Sequence Context AA Info Type MAF
rs960356 -- 123,968,398(+) attgc(C/T)tttgg intron-variant
rs989926 -- 123,941,175(-) tttac(A/G)GAATC intron-variant
rs1014439 -- 123,942,274(-) ACTTA(C/T)AGTTT intron-variant
rs1859402 -- 123,945,883(-) tgaat(A/G)agaat intron-variant
rs2057808 -- 123,947,497(-) tctta(G/T)ctcac intron-variant

Relevant External Links for SPAM1 Gene

HapMap Linkage Disequilibrium report
SPAM1

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Structural Variations from Database of Genomic Variants (DGV) for SPAM1 Gene

Disorders for SPAM1 Gene

(7) Novoseek inferred disease relationships for SPAM1 Gene

Disease -log(P) Hits PubMed IDs
bladder tumors 40 2
prostate cancer 23.1 6
bladder cancer 22.8 3
tumors 16 22
metastasis 11.1 3

Relevant External Links for SPAM1

Genetic Association Database (GAD)
SPAM1
Human Genome Epidemiology (HuGE) Navigator
SPAM1
genes like me logo Genes that share disorders with SPAM1: view

No data available for UniProtKB/Swiss-Prot for SPAM1 Gene

Publications for SPAM1 Gene

  1. The human sperm protein PH-20 has hyaluronidase activity. (PMID: 8282124) Gmachl M. … Kreil G. (FEBS Lett. 1993) 2 3 4 23
  2. Expression analysis, genomic structure, and mapping to 7q31 of the human sperm adhesion molecule gene SPAM1. (PMID: 8575780) Jones M.H. … Affara N.A. (Genomics 1995) 2 3 4 23
  3. Molecular cloning of the human and monkey sperm surface protein PH- 20. (PMID: 8234258) Lin Y. … Primakoff P. (Proc. Natl. Acad. Sci. U.S.A. 1993) 3 4 23
  4. A hyaluronidase activity of the sperm plasma membrane protein PH-20 enables sperm to penetrate the cumulus cell layer surrounding the egg. (PMID: 8195297) Lin Y. … Primakoff P. (J. Cell Biol. 1994) 3 23
  5. cDNA cloning reveals the molecular structure of a sperm surface protein, PH-20, involved in sperm-egg adhesion and the wide distribution of its gene among mammals. (PMID: 2269661) Lathrop W.F. … Primakoff P. (J. Cell Biol. 1990) 3 23

Products for SPAM1 Gene

Sources for SPAM1 Gene

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