Home
GeneCards Guide
Suite
- MalaCards
- GeneALaCart
- GeneDecks
- GeneIP
- GeneLoc
Terms and Conditions
- Terms of Use
- 3rd Party Notice
About Us
User Feedback
Mirror sites

Set Analyses:

Generates a file of GeneCards annotations for your list of genes

Advanced Search

Search By

Section (entire)

for

All GeneCards Within gene subset (to enable gene prioritization):
or upload a file of gene symbols

Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

SPAG9 Gene

protein-coding GIFtS: 56
GCID: GC17M049039

sperm associated antigen 9

Explore 17 diseases affiliated with
SPAG9 via

MalaCards

our new
Human Malady Compendium

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
Sperm Associated Antigen 9¹ ²		FLJ26141¹
CT89¹ ² ³		FLJ34602¹
JLP¹ ² ³		MGC117291¹
PHET¹ ² ³		MGC14967¹
KIAA0516¹ ³ ⁵		MGC74461¹
SYD1¹ ³ ⁵		JIP4²
HLC4¹ ²		C-Jun NH2-Terminal Kinase-Associated Leucine Zipper Protein²
PIG6¹ ²		C-Jun-Amino-Terminal Kinase-Interacting Protein 4²
HSS¹ ³		JNK Interacting Protein²
Cancer/Testis Antigen 89² ³		JNK/SAPK-Associated Protein²
Human Lung Cancer Oncogene 6 Protein² ³		Lung Cancer Oncogene 4²
Mitogen-Activated Protein Kinase 8-Interacting Protein 4² ³		Max-Binding Protein²
Protein Highly Expressed In Testis² ³		Proliferation-Inducing Gene 6²
Sperm Surface Protein² ³		MAPK8IP4³
Sunday Driver 1² ³		JNK-Associated Leucine-Zipper Protein³
HLC-6² ³		JNK-Interacting Protein 4³
JIP-4² ³		Proliferation-Inducing Protein 6³
FLJ13450¹		Sperm-Associated Antigen 9³
FLJ14006¹		Sperm-Specific Protein³

External Ids:	HGNC: 14524¹	Entrez Gene: 9043²	Ensembl: ENSG00000008294⁷	OMIM: 605430⁵	UniProtKB: O60271³

Export aliases for SPAG9 gene to outside databases

Previous GC identifers: GC17M048755 GC17M051523 GC17M049383 GC17M049515 GC17M046397 GC17M044409

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for SPAG9:

This gene encodes a member of the cancer testis antigen gene family. The encoded protein functions as a scaffold

protein that structurally organizes mitogen-activated protein kinases and mediates c-Jun-terminal kinase signaling.

This protein also binds to kinesin-1 and may be involved in microtubule-based membrane transport. This protein may

play a role in tumor growth and development. Alternate splicing results in multiple transcript variants. (provided by

RefSeq, Oct 2011)

UniProtKB/Swiss-Prot: JIP4_HUMAN, O60271

Function: The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by

aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Isoform 5 may play a

role in spermatozoa-egg-interaction

Gene Wiki entry for SPAG9

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000017.10 NC_018928.1 NT_010783.15

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the SPAG9 gene promoter:
ATF-2 Lmo2 Olf-1 XBP-1 NF-kappaB c-Jun ZIC2/Zic2 NF-kappaB1
Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 3): SPAG9 promoter sequence

Search SABiosciences Chromatin IP Primers for SPAG9

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat SPAG9

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 17q21.33 Ensembl cytogenetic band: 17q21.33 HGNC chromosome: 17

SPAG9 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
SPAG9 gene location

GeneLoc information about chromosome 17 GeneLoc Exon Structure

GeneLoc location for GC17M049039: view genomic region (about GC identifiers)

Start:	49,039,535 bp from pter	End:	49,198,226 bp from pter
Size:	158,692 bases	Orientation:	minus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: JIP4_HUMAN, O60271 (See protein sequence)

Recommended Name: C-Jun-amino-terminal kinase-interacting protein 4

Size: 1321 amino acids; 146205 Da

Subunit: Homooligomer. Interacts with MAX, MAPK8, MAPK9, MAPK10, MAPK14, MAP3K3, MYC, KNS2 and MAP2K4. Interaction with

KNS2 is important in the formation of ternary complex with MAPK8. Interacts with NFKB1

Subcellular location: Cytoplasm. Cytoplasm, perinuclear region. Note=Perinuclear distribution in response to stress

signals such as UV radiation

Subcellular location: Isoform 5: Cytoplasmic vesicle, secretory vesicle, acrosome. Note=Associated with the plasma

membrane of the acrosomal compartment and also localizes in the acrosome matrix

Sequence caution: Sequence=AAH07524.1; Type=Miscellaneous discrepancy; Note=Probable cloning artifact;

Sequence=AAH59946.1; Type=Miscellaneous discrepancy; Note=Probable cloning artifact; Sequence=AAI06049.1;

Type=Miscellaneous discrepancy; Note=Contaminating sequence. Sequence of unknown origin in the C-terminal part;

Sequence=AAO66462.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=BAA25442.3;

Type=Erroneous initiation; Note=Translation N-terminally shortened; Sequence=BAB14812.1; Type=Miscellaneous

discrepancy; Note=Unlikely isoform. Aberrant splicing; Sequence=CAA62987.1; Type=Erroneous initiation;

Note=Translation N-terminally extended;

1 PDB 3D structure from and Proteopedia for SPAG9:

2W83 (3D)

Secondary accessions: A6H8U5 A8MSX0 B4DHH2 O60905 Q3KQU8 Q3MKM7 Q86WC7 Q86WC8 Q8IZX7 Q96II0 Q9H811

Alternative splicing: 6 isoforms: O60271-1 O60271-2 O60271-3 O60271-4 O60271-5 O60271-9 (No experimental confirmation available. Ref.5 (BAG58134) sequence is in conflict in position: 9:F->S)

Explore the universe of human proteins at neXtProt for SPAG9: NX_O60271

Post-translational modifications:

Phosphorylated by MAPK8 and MAPK14 (By similarity)¹

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_O60271

SPAG9 Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins (4 alternative transcripts):

NP_001123999.1 NP_001124000.1 NP_001238900.1 NP_003962.3

ENSEMBL proteins:

ENSP00000262013 ENSP00000423165 ENSP00000426900 ENSP00000349636 ENSP00000423346

ENSP00000430205 ENSP00000429343 ENSP00000426061

Reactome Protein details: O60271
Human Recombinant Protein Products:

	Browse Purified and Recombinant Proteins at EMD Millipore
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Browse OriGene full length recombinant human proteins expressed in human HEK293 cells OriGene Protein Over-expression Lysate: SPAG9 OriGene Custom Protein Services for SPAG9
	GenScript Custom Purified and Recombinant Proteins Services for SPAG9
	Novus Biologicals SPAG9 Protein Novus Biologicals SPAG9 Lysate
	Browse Sino Biological Recombinant Proteins
	Browse ProSpec Recombinant Proteins
	Uscn Proteins for SPAG9

Gene Ontology (GO): 5 cellular component terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0001669	acrosomal vesicle	IEA	--
GO:0005737	cytoplasm	IDA	--
GO:0005829	cytosol	TAS	--
GO:0016021	integral to membrane	TAS	9480848
GO:0048471	perinuclear region of cytoplasm	IEA	--

SPAG9 for ontologies About GeneDecksing

SPAG9 Antibody Products:

	Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Browse R&D Systems for Antibodies
	Cell Signaling Technology (CST) Antibodies for SPAG9 (JIP4)
	Browse OriGene Antibodies OriGene Custom Antibody Services for SPAG9
	GenScript Custom Superior Antibodies Services for SPAG9
	Novus Biologicals SPAG9 Antibodies
	Search for Antibodies for SPAG9 at Abcam
	Uscn Antibodies for SPAG9
	ThermoFisher Antibodies for SPAG9

Assay Products for SPAG9:

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Immunoassay Development Browse OriGene Fluorogenic Cell Assay Kits
	Browse R&D Systems for biochemical assays
	GenScript Custom Assay Services for SPAG9
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for SPAG9

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

SPAG9 for domains About GeneDecksing

3 InterPro domains/families:

IPR019143 JNK/Rab-associated_protein-1_N

IPR017986 WD40_repeat_dom

IPR015943 WD40/YVTN_repeat-like_dom

Graphical View of Domain Structure for InterPro Entry O60271

ProtoNet protein and cluster: O60271

UniProtKB/Swiss-Prot: JIP4_HUMAN, O60271

Similarity: Belongs to the JIP scaffold family

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Function Summary:

UniProtKB/Swiss-Prot: JIP4_HUMAN, O60271

Function: The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by

aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Isoform 5 may play a

role in spermatozoa-egg-interaction

Induction: Isoform 3 is increased in systemic sclerosis fibroblasts

miRNA Products:		OriGene 3'-UTR Clone (see all 2): SPAG9 Browse MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat SPAG9
		8/101 QIAGEN miScript miRNA Assays for microRNAs that regulate SPAG9 (see all 101):
		hsa-miR-548j hsa-miR-607 hsa-miR-376b hsa-miR-200a hsa-miR-3653 hsa-miR-30d hsa-miR-371-5p hsa-miR-342-3p

		SwitchGear 3'UTR luciferase reporter plasmid: SPAG9 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for SPAG9 (see all 7) OriGene shRNA RFP: SPAG9 OriGene siRNA: SPAG9
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat SPAG9

Gene Editing
Products:

DNA2.0 Custom Protein Engineering Service for SPAG9

Clone Products:		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for SPAG9 (see all 8) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for SPAG9 (see all 4) OriGene custom cloning services � gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector (see all 2): SPAG9 (NM_003971)
		Browse Sino Biological Human cDNA Clones
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for SPAG9
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat SPAG9

Cell Line Products:		GenScript Custom overexpressing Cell Line Services for SPAG9
		Search LifeMap BioReagents cell lines for SPAG9

In Situ Assay
Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for SPAG9

Gene Ontology (GO): 5 molecular function terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005515	protein binding	--	--
GO:0008432	JUN kinase binding	IEA	--
GO:0019894	kinesin binding	IEA	--
GO:0043548	phosphatidylinositol 3-kinase binding	--	--
GO:0048273	mitogen-activated protein kinase p38 binding	IEA	--

SPAG9 for ontologies About GeneDecksing

1 GenomeRNAi human phenotype for SPAG9:

Decreased viability of wild-ty

Animal Models:
Mouse knock-out Spag9^tm1Ysok for SPAG9
3 MGI mutant phenotypes (inferred from 2 alleles) (MGI details for Spag9):

integument

pigmentation

reproductive system

SPAG9 for phenotypes About GeneDecksing

(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
About This Section

Unified GeneCards pathways About this table

See pathways by source

Super-pathway

contained gene-specific pathways

Activation of p38 alpha/beta MAPK

	Activation of p38 alpha/beta MAPK	1.00			CDO in myogenesis	0.55
	Nuclear translocation of p38 MAPK	0.94			Myogenesis	0.55

MAP Kinase Signaling

MAP Kinase Signaling

1.00

Arf6 trafficking events

Arf6 trafficking events

1.00

Axon guidance

Developmental Biology

0.69

Akt Signaling

p38 Signaling

0.60

Pathway sources
See GeneCards unified pathways
Show all pathways

1 Downloadable PowerPoint Slide of QIAGEN Pathway Central Maps for SPAG9

p38 Signaling

1 Cell Signaling Technology (CST) Pathway for SPAG9

MAP Kinase Signaling

1 BioSystems Pathway for SPAG9

Arf6 trafficking events

5 Reactome Pathways for SPAG9

	Activation of p38 alpha/beta MAPK
	Developmental Biology
	Myogenesis
	Nuclear translocation of p38 MAPK
	CDO in myogenesis

SPAG9 for pathways About GeneDecksing

Interactions:

SABiosciences Gene Network Central^TM Interacting Genes and Proteins Network for SPAG9

STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

5/48 Interacting proteins for SPAG9 (O60271^{2, 3} ENSP00000262013⁴) via UniProtKB, MINT, STRING, and/or I2D (see all 48)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
ARF6	P62330²^,³, ENSP00000298316⁴	MINT-7922449 MINT-7922407 MINT-7922481 MINT-7922423 MINT-7922462 MINT-7922498 MINT-7915283 MINT-7922515 I2D: score=3 STRING: ENSP00000298316
NFKB1	P19838²^,³, ENSP00000226574⁴	MINT-48540 MINT-47889 MINT-48560 I2D: score=4 STRING: ENSP00000226574
ARF1	P84077²^,³, ENSP00000272102⁴	MINT-7922576 MINT-7922541 MINT-7922558 I2D: score=1 STRING: ENSP00000272102
PNRC2	Q9NPJ4²^,³, ENSP00000334840⁴	MINT-8267030 I2D: score=2 STRING: ENSP00000334840
LOC100996626	Q9NPJ4²^,³	MINT-8267030 I2D: score=2

About this table

Gene Ontology (GO): 5/12 biological process terms (GO ID links to tree view) (see all 12): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0000187	activation of MAPK activity	--	--
GO:0007257	activation of JUN kinase activity	IEA	--
GO:0007283	spermatogenesis	TAS	9480848
GO:0030335	positive regulation of cell migration	IMP	18826971
GO:0042147	retrograde transport, endosome to Golgi	IDA	19056739

SPAG9 for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section

SPAG9 for compounds About GeneDecksing

Browse Small Molecules at EMD Millipore

Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for SPAG9
6 Novoseek chemical compound relationships for SPAG9 gene About this table

Compound	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
sp(1-7)	88.6	5	9780359 (1), 7525387 (1), 12530914 (1), 7578682 (1) (see all 5)
leucine	32.2	11	15693750 (2), 18826971 (2), 16077255 (1), 12391307 (1)
phosphotyrosine	25.8	3	12390886 (2), 9047024 (1)
biotin	20.6	2	1426351 (1), 9047024 (1)
calcium	13.7	4	12390886 (2), 12566158 (1), 15579586 (1)
tyrosine	0	3	16219692 (1), 15223846 (1)

Search CenterWatch for drugs/clinical trials and news about SPAG9 / JIP4

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from EMD Millipore,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
About This Section

REFSEQ mRNAs for SPAG9 gene (5 alternative transcripts):

NM_001130527.2 NM_001130528.2 NM_001251971.1 NM_003971.5 NM_172345.1

Unigene Cluster for SPAG9:

Sperm associated antigen 9

Hs.463439 [show with all ESTs]

Unigene Representative Sequence: NM_001130528

18/21 Ensembl transcripts including schematic representations, and UCSC links where relevant (see all 21):

ENST00000262013(uc002ita.3 uc002itc.3 uc002itd.3 uc002itb.3)

ENST00000509724 ENST00000510283 ENST00000505279 ENST00000357122 ENST00000506500

ENST00000513746 ENST00000511312 ENST00000513906 ENST00000514613(uc002ite.3 uc002itf.3)

ENST00000505173 ENST00000514205 ENST00000506483 ENST00000513827 ENST00000515685

ENST00000513547 ENST00000511795 ENST00000576492

miRNA Products:		OriGene 3'-UTR Clone (see all 2): SPAG9 Browse OriGene MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat SPAG9
		8/101 QIAGEN miScript miRNA Assays for microRNAs that regulate SPAG9 (see all 101):
		hsa-miR-548j hsa-miR-607 hsa-miR-376b hsa-miR-200a hsa-miR-3653 hsa-miR-30d hsa-miR-371-5p hsa-miR-342-3p

		SwitchGear 3'UTR luciferase reporter plasmid: SPAG9 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for SPAG9 (see all 7) OriGene shRNA RFP: SPAG9 OriGene siRNA: SPAG9
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat SPAG9

Clone Products:		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for SPAG9 (see all 8) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for SPAG9 (see all 4) OriGene custom cloning services � gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector (see all 2): SPAG9 (NM_003971)
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for SPAG9
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat SPAG9

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for SPAG9 Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat SPAG9
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat SPAG9
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat SPAG9

Additional cDNA sequence:

AB011088.3 AF327452.1 AF542172.1 AK023512.1 AK091921.1 AK129652.1 AK295098.1 AK299553.1

AK302789.1 AK307793.1 AK309214.1 AY219897.1 AY219898.1 AY236487.1 AY850123.1 BC007524.1

BC059946.1 BC146755.1 BC153878.1 X91879.1

23 DOTS entries:

DT.100796546 DT.95378814 DT.212964 DT.445501 DT.101956305 DT.75107274 DT.97843947 DT.97812510

DT.97844752 DT.120918771 DT.91763028 DT.406687 DT.100796538 DT.120918714 DT.120918762 DT.120918761

DT.120918691 DT.120918693 DT.120918726 DT.120918765 DT.97843946 DT.120918737 DT.91826864

24/380 AceView cDNA sequences (see all 380):

AA227958 BM479860 BM832541 AK091921 AK023512 CB141432 AI758541 AB011088

CA429748 AU143428 AI816917 AX747254 AK024068 BQ774865 BU629444 BM853161

BU631965 AI276428 AA719651 AI110819 BQ425325 AA586494 BQ431712 H88922

GeneLoc Exon Structure

5/14 Alternative Splicing Database (ASD) splice patterns (SP) for SPAG9 (see all 14) About this scheme

ExUns:	1a	·	1b	^	2	^	3	^	4a	·	4b	·	4c	^	5	^	6a	·	6b	^	7a	·	7b	^	8	^	9a	·	9b	^	10	^	11	^	12	^	13	^	14	^	15a	·	15b	·	15c	^	16a	·	16b	^	17a	·
SP1:									-		-		-						-																														-		-
SP2:									-		-		-				-		-																														-		-
SP3:									-		-		-				-		-																														-		-
SP4:									-		-		-						-																														-		-
SP5:																																																	-		-

ExUns:	17b	^	18	^	19a	·	19b	^	20a	·	20b	·	20c	^	21	^	22a	·	22b	·	22c	·	22d	^	23	^	24	^	25	^	26a	·	26b	^	27	^	28a	·	28b	^	29	^	30a	·	30b	^	31	^	32a	·	32b	·
SP1:									-				-		-						-		-																-		-		-
SP2:									-				-		-						-		-																-		-		-
SP3:									-				-		-
SP4:									-				-		-
SP5:									-				-		-						-		-																-				-

ExUns:	32c	·	32d
SP1:
SP2:
SP3:
SP4:
SP5:

ECgene alternative splicing isoforms for SPAG9

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

SPAG9 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: GTGGCAGACA

About this image
See SPAG9 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for SPAG9

SOURCE GeneReport for Unigene cluster: Hs.463439

UniProtKB/Swiss-Prot: JIP4_HUMAN, O60271

Tissue specificity: Isoform 5 is expressed only in testis on the round spermatids of stage I, II and II. Isoform 5 is

absent in spermatogonia and spermatocyte. Isoform 3 is expressed in testis. Isoform 4 is expressed in testis and in

acute myeloid leukemia (AML) patients

SABiosciences Custom PCR Arrays for SPAG9

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for SPAG9 Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat SPAG9
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat SPAG9
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat SPAG9

In Situ
Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for SPAG9

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the common ancestor of animals.

Orthologs for SPAG9 gene from 6/20 species (see all 20) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
chicken (Gallus gallus)	Aves	SPAG9¹	sperm associated antigen 9	82.1(n) 89(a)		422095 XM_420098.3 XP_420098.3
lizard (Anolis carolinensis)	Reptilia	SPAG9⁶	--	84(a)	1 ↔ 1	2(117641057-117739729)
tropical clawed frog (Xenopus tropicalis)	Amphibia	Str.1719²	Transcribed sequence with moderate similarity to protein more	75.33(n)		AL852414.2
zebrafish (Danio rerio)	Actinopterygii	LOC100333761¹	c-Jun-amino-terminal kinase-interacting protein 4-like	65.77(n) 71(a)		100333761 XM_002663870.3 XP_002663916.2
fruit fly (Drosophila melanogaster)	Insecta	syd¹	sunday driver	52.18(n) 49.96(a)		43905 NM_079913.2 NP_524652.1
worm (Caenorhabditis elegans)	Secernentea	unc-16¹	Protein UNC-16	48.53(n) 40.61(a)		176349 NM_171221.3 NP_741263.3

ENSEMBL Gene Tree for SPAG9 (if available)
TreeFam Gene Tree for SPAG9 (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
About This Section

Paralogs for SPAG9 gene

ECT2² ARHGEF10² ARHGEF17² ARHGEF10L² MAPK8IP3²

2 SIMAP similar genes for SPAG9 using alignment to 5 protein entries: JIP4_HUMAN (see all proteins):

HLC4 MAPK8IP3

SPAG9 for paralogs About GeneDecksing

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section

SNP ID	Valid	Clinical significance	Chr 17 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs1006702^1,2	C,	--	44409254(+)	`TGGACG/TTGCAA`	4	--	ds500¹		0	--	--	--	--
rs1004501^1,2	C,F,O,	--	44409311(+)	`ACACAC/TTAGAC`	4	--	ds500¹		11		NA WA CSA	392
rs9899707^1,2	C,F,H,	--	44409869(+)	`TTCTTT/CAGGAC`	4	--	ut3¹		11		NS EA NA WA	1314
rs34084087^1,2		--	44410123(+)	`CACATA/GGGTTA`	4	--	ut3¹		0	--	--	--	--
rs7222309^1,2	C,F,A,H,	--	44411395(+)	`GGCCTT/CTATCC`	4	--	ut3¹		11		NS EA NA WA	1318
rs117729382^1,2	C,F,	--	44412267(+)	`CTTTAT/CGGTAT`	4	--	ut3¹		1		EA	120
rs8125^1,2	C,F,O,H,	--	44413134(-)	`NNNNGG/ATTTAC`	4	--	ut3¹		18		MN NS EA NA	2226
rs111489635^1,2	F	--	44413218(+)	`TACCTG/ATAACA`	4	--	ut3¹		1		CSA	4
rs76972510^1,2	C,F,	--	44413321(+)	`GTGGTG/AAAACT`	4	--	ut3¹		2		WA NA	238
rs78313028^1,2	F,	--	44413670(+)	`AATAGT/CAGAAG`	4	--	int¹		1		WA	118

HapMap Linkage Disequilibrium report for SPAG9 (49039535 - 49198226 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 1 variation for SPAG9
1 CNV: 5018

SABiosciences Cancer Mutation PCR Assays

QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing SPAG9

DNA2.0 Custom Variant and Variant Library Synthesis for SPAG9

(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

SPAG9 for disorders           About GeneDecksing

OMIM gene information: 605430    OMIM disorders: --

17 diseases for SPAG9:    About MalaCards

lung cancer myeloid leukemia chronic myeloid leukemia acute myeloid leukemia

epithelial ovarian cancer dengue disease renal cell carcinoma ovarian cancer

infertility epididymitis leukemia alzheimer's disease

thyroid cancer nasopharyngitis thyroiditis carcinoma

neuronitis

1 disease from the University of Copenhagen DISEASES database for SPAG9:

Dengue disease

2 Novoseek disease relationships for SPAG9 gene About this table

Disease	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
infertility	56.2	5	1550425 (1), 2383616 (1), 10336018 (1), 18980760 (1) (see all 5)
autoimmunity	4.58	1	1514025 (1)

Export disorders for SPAG9 gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
About This Section

PubMed articles for SPAG9 gene, integrated from 9 sources (see all 122):
(articles sorted by number of sources associating them with SPAG9)

Utopia: connect your pdf to the dynamic
world of online information

Cloning of a novel human testis mRNA specifically expressed in testicular haploid germ cells, having unique palindromic sequences and encoding a leucine zipper dimerization motif. (PubMed id 9480848)^{1, 2, 3, 9} Shankar S.... Suri A. (1998)
Characterization of a novel human sperm-associated antigen 9 (SPAG9) having structural homology with c-Jun N-terminal kinase-interacting protein. (PubMed id 15693750)^{1, 2, 9} Jagadish N.... Suri A. (2005)
A novel protein highly expressed in testis is overexpressed in systemic sclerosis fibroblasts and targeted by autoantibodies. (PubMed id 14662895)^{1, 2, 9} Yasuoka H.... Kuwana M. (2003)
JLP: a scaffolding protein that tethers JNK/p38MAPK signaling modules and transcription factors. (PubMed id 12391307)^{1, 2, 9} Lee C.M.... Reddy E.P. (2002)
Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PubMed id 17081983)^{1, 2} Olsen J.V....Mann M. (2006)
Humoral detection of leukaemia-associated antigens in presentation acute myeloid leukaemia. (PubMed id 16112646)^{1, 2} Guinn B.-A.... Mufti G.J. (2005)
Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)^{1, 2} Ota T.... Sugano S. (2004)
Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry. (PubMed id 15144186)^{1, 2} Brill L.M....Peters E.C. (2004)
A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway. (PubMed id 14743216)^{1, 2} Bouwmeester T....Superti-Furga G. (2004)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{1, 2} Gerhard D.S....Malek J. (2004)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

		AND	OR
Aliases
Disorders
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section

Entrez Gene: 9043	HGNC: 14524	AceView: SPAG9	Ensembl:ENSG00000008294	euGenes: HUgn9043
ECgene: SPAG9	H-InvDB: SPAG9

(According to HUGE)
About This Section

HUGE: KIAA0516

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Name	Description
PharmGKB entry for SPAG9	Pharmacogenomics, SNPs, Pathways

Intellectual Property for SPAG9 gene
(Patent information from GeneIP, Licensable technologies from WIS Yeda, Salk, Tufts, IP news from LifeMap Sciences, Inc.)

About This Section

Patent Information for SPAG9 gene:
Search GeneIP for patents involving SPAG9

GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search

(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section


	EMD Millipore Custom Antibody & Bulk Services
	EMD Millipore Preclinical / Clinical Development Services
	EMD Millipore Immunoassay Services
	EMD Millipore Target Screening & Profiling Services

	Browse Antibodies		Browse Cell Culture Products
	Browse ELISAs		Browse Flow Cytometry Kits
	Browse Primer Pairs		Browse Kinase Activity Assays/Reagents
	Browse ELISpot Kits/Development Modules		Browse TFB/Immunoprecipitation Assays
	Browse Apoptosis Detection Kits/Reagents		Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents
	Browse DNA Damage/Repair Kits/Reagents		Browse Multiplex/Array Assay Kits/Reagents
	Browse Cell Selection/Detection Kits/Reagents		Browse Secondary Antibodies/Controls/Staining Reagents
	Browse Phosphatase Activity Assays/Reagents		Browse Recombinant/Natural Proteins

	Browse OriGene Antibodies		OriGene shRNA RFP for SPAG9
	OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for SPAG9		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for SPAG9
	OriGene Protein Over-expression Lysate for SPAG9		Browse OriGene Fluorogenic Cell Assay Kits
	OriGene siRNA for SPAG9		OriGene 3'-UTR Clone for SPAG9
	OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for SPAG9		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for SPAG9
	Browse OriGene GFP tagged cDNA clones in CMV expression vector		Browse OriGene MicroRNA Expression Plasmids
	Browse OriGene basic RS shRNAs		Browse OriGene validated miRNA SYBR primer pairs
	Browse OriGene full length recombinant human proteins expressed in human HEK293 cells		OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
	OriGene Custom Antibody Services for SPAG9		OriGene Custom Protein Services for SPAG9
	OriGene Custom Immunoassay Development

	QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat SPAG9
	QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing SPAG9
	QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat SPAG9
	QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat SPAG9
	QIAGEN QuantiFast Probe-based Assays in human, mouse, rat SPAG9
	QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat SPAG9

	GenScript Custom Purified and Recombinant Proteins Services for SPAG9		GenScript cDNA clones with any tag delivered in your preferred vector for SPAG9
	GenScript Custom Assay Services for SPAG9		GenScript Custom Superior Antibodies Services for SPAG9
	GenScript Custom overexpressing Cell Line Services for SPAG9		CloneReady with Over 120,000 Genes
	Gene Synthesis: Any Gene in Any Vector		Vector-based siRNA and miRNA, Ready for Transfection
	Gene Mutant Library, Variants up to 10^11		Plasmid Preparation
	Custom Peptide Services

Antibodies & Assays for SPAG9 (JIP4)

	Regulatory tfbs in SPAG9 promoter
	Search Chromatin IP Primers for SPAG9
	RT² qPCR Primer Assay in human, mouse, rat SPAG9
	GNC Network for SPAG9
	SABiosciences PCR Arrays including human, mouse, rat SPAG9

Search Tocris compounds for SPAG9

	Browse Sino Biological Proteins and Antibodies
	Browse Sino Biological cDNA Clones
	Antibodies/Proteins Production Services
	Rabbit Monoclonal Antibody Platform
	Bulk Purchasing

Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

	Novus Tissue Slides
	SPAG9 antibodies
	SPAG9 proteins
	SPAG9 lysates

	Search for Antibodies for SPAG9 at Abcam
	See all of Abcam's Antibodies, Kits and Proteins for SPAG9
	Custom Antibody / Protein Production Service
	Bulk Purchasing
	Advantages of Rabbit Monoclonal antibodies
	Abcam protocols and scientific support

Browse ProSpec Recombinant Proteins

SPAG9 Proteins, Antibodies, CLIAs, and ELISAs

Search LifeMap BioReagents cell lines for SPAG9

	Gene Synthesis
	Protein Engineering
	Variant Library Synthesis
	Codon Optimization
	Protein Production and Purification

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for SPAG9

	SwitchGear 3'UTR luciferase reporter plasmids for SPAG9
	SwitchGear Promoter luciferase reporter plasmids for SPAG9

ThermoFisher Antibodies for SPAG9

Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat SPAG9

GeneCards Homepage - Last full update: 19 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 26 Apr 2013

View Random Gene

Category			VWF (GIFTS: 73) von Willebrand factor
GIFtS Group

The GeneCards human gene database gene index: 1 3 5 6 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

The GeneCards Human Gene Database

Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

Hot genes Disease genes SPAG9 gene at Home site.

hostname: 356980-web2.xennexinc.com index build: 100 solr: 1.4

Advanced Search

Export list of displayed genes

SPAG9 Gene

sperm associated antigen 9

Aliases
for SPAG9 gene

Summaries
for SPAG9 gene

Genomic Views
for SPAG9 gene

Proteins
for SPAG9 gene

Protein Domains /
Families
for SPAG9 gene

Function
for SPAG9 gene

Pathways & Interactions
for SPAG9 gene

Drugs & Compounds
for SPAG9 gene

Transcripts
for SPAG9 gene

Expression
for SPAG9 gene

Orthologs
for SPAG9 gene

Paralogs
for SPAG9 gene

Genomic Variants
for SPAG9 gene

Disorders / Diseases
for SPAG9 gene

Publications
for SPAG9 gene

External Searches for SPAG9 gene

Genome Databases showing SPAG9 gene

Other Databases showing SPAG9 gene

Specialized Databases showing SPAG9 gene

Intellectual Property
for SPAG9 gene

Products
for SPAG9 gene

View Random Gene

SPAG9 (Gene Symbol)
Sperm Associated Antigen 9
CT89
JLP
PHET
KIAA0516
SYD1
HLC4
PIG6
HSS
Cancer/Testis Antigen 89
Human Lung Cancer Oncogene 6 Protein
Mitogen-Activated Protein Kinase 8-Interacting Protein 4
Protein Highly Expressed In Testis
Sperm Surface Protein
Sunday Driver 1
HLC-6
JIP-4
FLJ13450
FLJ14006
FLJ26141
FLJ34602
MGC117291
MGC14967
MGC74461
JIP4
C-Jun NH2-Terminal Kinase-Associated Leucine Zipper Protein
C-Jun-Amino-Terminal Kinase-Interacting Protein 4
JNK Interacting Protein
JNK/SAPK-Associated Protein
Lung Cancer Oncogene 4
Max-Binding Protein
Proliferation-Inducing Gene 6
MAPK8IP4
JNK-Associated Leucine-Zipper Protein
JNK-Interacting Protein 4
Proliferation-Inducing Protein 6
Sperm-Associated Antigen 9
Sperm-Specific Protein

Advanced Search

Export list of displayed genes

SPAG9 Gene

sperm associated antigen 9

Aliasesfor SPAG9 gene

Summariesfor SPAG9 gene

Genomic Viewsfor SPAG9 gene

Proteinsfor SPAG9 gene

Protein Domains /Familiesfor SPAG9 gene

Functionfor SPAG9 gene

Pathways & Interactionsfor SPAG9 gene

Drugs & Compoundsfor SPAG9 gene

Transcriptsfor SPAG9 gene

Expression for SPAG9 gene

Orthologsfor SPAG9 gene

Paralogsfor SPAG9 gene

Genomic Variantsfor SPAG9 gene

Disorders / Diseasesfor SPAG9 gene

Publicationsfor SPAG9 gene

External Searches for SPAG9 gene

Genome Databases showing SPAG9 gene

Other Databases showing SPAG9 gene

Specialized Databases showing SPAG9 gene

Intellectual Propertyfor SPAG9 gene

Productsfor SPAG9 gene

View Random Gene

Aliases
for SPAG9 gene

Summaries
for SPAG9 gene

Genomic Views
for SPAG9 gene

Proteins
for SPAG9 gene

Protein Domains /
Families
for SPAG9 gene

Function
for SPAG9 gene

Pathways & Interactions
for SPAG9 gene

Drugs & Compounds
for SPAG9 gene

Transcripts
for SPAG9 gene

Expression
for SPAG9 gene

Orthologs
for SPAG9 gene

Paralogs
for SPAG9 gene

Genomic Variants
for SPAG9 gene

Disorders / Diseases
for SPAG9 gene

Publications
for SPAG9 gene

Intellectual Property
for SPAG9 gene

Products
for SPAG9 gene