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Aliases for SPAG8 Gene

Aliases for SPAG8 Gene

  • Sperm Associated Antigen 8 2 3 5
  • Sperm Membrane Protein BS-84 3 4
  • Sperm Membrane Protein 1 3 4
  • HSD-1 3 4
  • Testicular Tissue Protein Li 177 3
  • CILD28 3
  • HSMP-1 3
  • BS-84 3
  • CT142 3
  • SPAG3 3
  • SMP-1 4
  • SMP1 3

External Ids for SPAG8 Gene

Previous GeneCards Identifiers for SPAG8 Gene

  • GC09M036120
  • GC09M035977

Summaries for SPAG8 Gene

Entrez Gene Summary for SPAG8 Gene

  • The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein encoded by this gene is recognized by sperm agglutinating antibodies from an infertile woman. This protein is localized in germ cells of the testis at all stages of spermatogenesis and is localized to the acrosomal region of mature spermatozoa. This protein interacts with ACT (activator of CREM in testis) and may play a role in CREM (cAMP response element modulator)-ACT-mediated gene transcription during spermatogenesis. This protein may also play a role in spermatogenesis by regulating microtubule formation and cell division. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined. [provided by RefSeq, Jul 2012]

GeneCards Summary for SPAG8 Gene

SPAG8 (Sperm Associated Antigen 8) is a Protein Coding gene. GO annotations related to this gene include microtubule binding.

UniProtKB/Swiss-Prot for SPAG8 Gene

  • May play a role in fertility and microtubule formation through interaction with RANBP9.

Gene Wiki entry for SPAG8 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for SPAG8 Gene

Genomics for SPAG8 Gene

Regulatory Elements for SPAG8 Gene

Promoters for SPAG8 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around SPAG8 on UCSC Golden Path with GeneCards custom track

Genomic Location for SPAG8 Gene

Chromosome:
9
Start:
35,807,785 bp from pter
End:
35,812,272 bp from pter
Size:
4,488 bases
Orientation:
Minus strand

Genomic View for SPAG8 Gene

Genes around SPAG8 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
SPAG8 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for SPAG8 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for SPAG8 Gene

Proteins for SPAG8 Gene

  • Protein details for SPAG8 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q99932-SPAG8_HUMAN
    Recommended name:
    Sperm-associated antigen 8
    Protein Accession:
    Q99932
    Secondary Accessions:
    • B4DY89
    • E9PDV6
    • Q12937
    • Q5TCV8
    • Q8WWB4

    Protein attributes for SPAG8 Gene

    Size:
    426 amino acids
    Molecular mass:
    44819 Da
    Quaternary structure:
    • Interacts with RANBP9.
    SequenceCaution:
    • Sequence=AAB46833.1; Type=Erroneous termination; Positions=427; Note=Translated as stop.; Evidence={ECO:0000305};

    Alternative splice isoforms for SPAG8 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for SPAG8 Gene

Proteomics data for SPAG8 Gene at MOPED

Post-translational modifications for SPAG8 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Antibody Products

  • Santa Cruz Biotechnology (SCBT) Antibodies for SPAG8

No data available for DME Specific Peptides for SPAG8 Gene

Domains & Families for SPAG8 Gene

Protein Domains for SPAG8 Gene

InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for SPAG8 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

genes like me logo Genes that share domains with SPAG8: view

No data available for Gene Families and UniProtKB/Swiss-Prot for SPAG8 Gene

Function for SPAG8 Gene

Molecular function for SPAG8 Gene

UniProtKB/Swiss-Prot Function:
May play a role in fertility and microtubule formation through interaction with RANBP9.

Gene Ontology (GO) - Molecular Function for SPAG8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003674 molecular_function ND --
genes like me logo Genes that share ontologies with SPAG8: view

Phenotypes for SPAG8 Gene

GenomeRNAi human phenotypes for SPAG8:
genes like me logo Genes that share phenotypes with SPAG8: view

Animal Model Products

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , miRNA , Transcription Factor Targets and HOMER Transcription for SPAG8 Gene

Localization for SPAG8 Gene

Subcellular locations from UniProtKB/Swiss-Prot for SPAG8 Gene

Cytoplasmic vesicle, secretory vesicle, acrosome. Note=Acrosomal region of the head of mature sperm cells.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for SPAG8 Gene COMPARTMENTS Subcellular localization image for SPAG8 gene
Compartment Confidence
nucleus 4
cytosol 2

Gene Ontology (GO) - Cellular Components for SPAG8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IEA,IBA --
genes like me logo Genes that share ontologies with SPAG8: view

Pathways & Interactions for SPAG8 Gene

SuperPathways for SPAG8 Gene

No Data Available

Gene Ontology (GO) - Biological Process for SPAG8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0032092 positive regulation of protein binding IEA --
genes like me logo Genes that share ontologies with SPAG8: view

No data available for Pathways by source and SIGNOR curated interactions for SPAG8 Gene

Drugs & Compounds for SPAG8 Gene

No Compound Related Data Available

Transcripts for SPAG8 Gene

Unigene Clusters for SPAG8 Gene

Sperm associated antigen 8:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for SPAG8 Gene

ExUns: 1a · 1b · 1c · 1d ^ 2a · 2b · 2c ^ 3 ^ 4 ^ 5a · 5b · 5c ^ 6a · 6b ^ 7a · 7b · 7c
SP1: - - -
SP2: - -
SP3: -
SP4: -
SP5: - -
SP6:

Relevant External Links for SPAG8 Gene

GeneLoc Exon Structure for
SPAG8
ECgene alternative splicing isoforms for
SPAG8

Expression for SPAG8 Gene

mRNA expression in normal human tissues for SPAG8 Gene

mRNA differential expression in normal tissues according to GTEx for SPAG8 Gene

This gene is overexpressed in Testis (x5.5).

SOURCE GeneReport for Unigene cluster for SPAG8 Gene Hs.256747

mRNA Expression by UniProt/SwissProt for SPAG8 Gene

Q99932-SPAG8_HUMAN
Tissue specificity: Expressed in testis (germ cells), but not in liver, kidney, prostate and small intestine.
genes like me logo Genes that share expression patterns with SPAG8: view

Primer Products

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , Protein differential expression in normal tissues , Protein expression and Protein tissue co-expression partners for SPAG8 Gene

Orthologs for SPAG8 Gene

This gene was present in the common ancestor of chordates.

Orthologs for SPAG8 Gene

Organism Taxonomy Gene Similarity Type Details
dog
(Canis familiaris)
Mammalia SPAG8 35
  • 81.36 (n)
  • 72.99 (a)
SPAG8 36
  • 62 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Spag8 35
  • 69.48 (n)
  • 60.42 (a)
Spag8 16
Spag8 36
  • 58 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia SPAG8 35
  • 98.27 (n)
  • 97.51 (a)
SPAG8 36
  • 98 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Spag8 35
  • 73.6 (n)
  • 60.51 (a)
cow
(Bos Taurus)
Mammalia SPAG8 36
  • 56 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia SPAG8 36
  • 35 (a)
OneToOne
chicken
(Gallus gallus)
Aves SPAG8 36
  • 40 (a)
OneToOne
sea squirt
(Ciona savignyi)
Ascidiacea CSA.10155 36
  • 28 (a)
OneToOne
Species with no ortholog for SPAG8:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • lizard (Anolis carolinensis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • oppossum (Monodelphis domestica)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)
  • zebrafish (Danio rerio)

Evolution for SPAG8 Gene

ENSEMBL:
Gene Tree for SPAG8 (if available)
TreeFam:
Gene Tree for SPAG8 (if available)

Paralogs for SPAG8 Gene

No data available for Paralogs for SPAG8 Gene

Variants for SPAG8 Gene

Sequence variations from dbSNP and Humsavar for SPAG8 Gene

SNP ID Clin Chr 09 pos Sequence Context AA Info Type
rs13299596 - 35,811,841(+) AGCTG(A/C/T)TTTGG reference, missense
rs17851728 - 35,811,369(+) AGTTA(A/G)GGACC reference, missense
rs3830980 -- 35,812,115(+) CGCGA(-/CCGCGA)TCCCT cds-indel
rs11791045 -- 35,811,553(+) GCCAC(A/G)GCCAG reference, missense
rs28690455 -- 35,811,570(+) AGCCA(G/T)AGCCA reference, missense

Structural Variations from Database of Genomic Variants (DGV) for SPAG8 Gene

Variant ID Type Subtype PubMed ID
dgv8168n71 CNV Gain 21882294

Variation tolerance for SPAG8 Gene

Residual Variation Intolerance Score: 71.4% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 11.50; 92.92% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for SPAG8 Gene

HapMap Linkage Disequilibrium report
SPAG8

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for SPAG8 Gene

Disorders for SPAG8 Gene

Relevant External Links for SPAG8

Atlas of Genetics and Cytogenetics in Oncology and Haematology:
SPAG8

No disorders were found for SPAG8 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for SPAG8 Gene

Publications for SPAG8 Gene

  1. Assignment of chromosomal locus and evidence for alternatively spliced mRNAs of a human sperm membrane protein (hSMP-1). (PMID: 10500252) Wang H. … Koide S.S. (Biochim. Biophys. Acta 1999) 2 3 4 23 67
  2. Expression and characterization of a novel human sperm membrane protein. (PMID: 8788182) Liu Q.-Y. … Catterall J.F. (Biol. Reprod. 1996) 2 3 23
  3. Regulation of the G2/M phase of the cell cycle by sperm associated antigen 8 (SPAG8) protein. (PMID: 19548270) Li R. … Wang L.F. (Cell Biochem. Funct. 2009) 3 23
  4. Inhibition of mouse acrosome reaction and sperm-zona pellucida binding by anti-human sperm membrane protein 1 antibody. (PMID: 17187156) Cheng G.Y. … Xu C. (Asian J. Androl. 2007) 3 23
  5. Human sperm membrane protein (hSMP-1): a developmental testis-specific component during germ cell differentiation. (PMID: 11111873) Zhang X.D. … Koide S.S. (Arch. Androl. 2000) 3 23

Products for SPAG8 Gene

Sources for SPAG8 Gene

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