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Aliases for SPAG6 Gene

Aliases for SPAG6 Gene

  • Sperm Associated Antigen 6 2 3
  • Axoneme Central Apparatus Protein 2 3
  • Sperm Flagellar Protein 3 4
  • Protein PF16 Homolog 3 4
  • Repro-SA-1 3 4
  • Pf16 3 4
  • CT141 3

External Ids for SPAG6 Gene

Previous GeneCards Identifiers for SPAG6 Gene

  • GC10P022369
  • GC10P022784
  • GC10P022638
  • GC10P022674
  • GC10P022634

Summaries for SPAG6 Gene

Entrez Gene Summary for SPAG6 Gene

  • The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm antibodies from an infertile man. This protein localizes to the tail of permeabilized human sperm and contains eight contiguous armadillo repeats, a motif known to mediate protein-protein interactions. Studies in mice suggest that this protein is involved in sperm flagellar motility and maintenance of the structural integrity of mature sperm. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

GeneCards Summary for SPAG6 Gene

SPAG6 (Sperm Associated Antigen 6) is a Protein Coding gene. Diseases associated with SPAG6 include hydrocephalus. GO annotations related to this gene include binding. An important paralog of this gene is KPNA1.

UniProtKB/Swiss-Prot for SPAG6 Gene

  • Important for structural integrity of the central apparatus in the sperm tail and for flagellar motility.

Gene Wiki entry for SPAG6 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for SPAG6 Gene

Genomics for SPAG6 Gene

Regulatory Elements for SPAG6 Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for SPAG6 Gene

22,345,416 bp from pter
22,454,224 bp from pter
108,809 bases
Plus strand

Genomic View for SPAG6 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for SPAG6 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for SPAG6 Gene

Proteins for SPAG6 Gene

  • Protein details for SPAG6 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Sperm-associated antigen 6
    Protein Accession:
    Secondary Accessions:
    • A8K1I8
    • B4DXZ4
    • Q5VUX5
    • Q5VUX6
    • Q5VUX7
    • Q6FI74
    • Q8NHQ6

    Protein attributes for SPAG6 Gene

    509 amino acids
    Molecular mass:
    55476 Da
    Quaternary structure:
    • Interacts with SPAG16 and SPAG17.

    Alternative splice isoforms for SPAG6 Gene


neXtProt entry for SPAG6 Gene

Proteomics data for SPAG6 Gene at MOPED

Post-translational modifications for SPAG6 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Antibody Products

No data available for DME Specific Peptides for SPAG6 Gene

Domains & Families for SPAG6 Gene

Gene Families for SPAG6 Gene

Protein Domains for SPAG6 Gene

Graphical View of Domain Structure for InterPro Entry



  • Contains 8 ARM repeats.
  • Contains 8 ARM repeats.
genes like me logo Genes that share domains with SPAG6: view

Function for SPAG6 Gene

Molecular function for SPAG6 Gene

GENATLAS Biochemistry:
sperm associated antigen 6,highly expressed in testis located to the tails of permeabilized human sperm but mediating protein-protein interacting
UniProtKB/Swiss-Prot Function:
Important for structural integrity of the central apparatus in the sperm tail and for flagellar motility.

Gene Ontology (GO) - Molecular Function for SPAG6 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005488 binding --
GO:0005515 protein binding --
genes like me logo Genes that share ontologies with SPAG6: view
genes like me logo Genes that share phenotypes with SPAG6: view

Animal Model Products

No data available for Enzyme Numbers (IUBMB) , Animal Models , miRNA , Transcription Factor Targets and HOMER Transcription for SPAG6 Gene

Localization for SPAG6 Gene

Subcellular locations from UniProtKB/Swiss-Prot for SPAG6 Gene

Cytoplasm, cytoskeleton. Cell projection, cilium, flagellum. Note=Associated with microtubules. Detected on the sperm flagellum.

Subcellular locations from

Jensen Localization Image for SPAG6 Gene COMPARTMENTS Subcellular localization image for SPAG6 gene
Compartment Confidence
nucleus 5
cytoskeleton 4

Gene Ontology (GO) - Cellular Components for SPAG6 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IDA 21630459
GO:0005874 microtubule IEA --
GO:0005930 axoneme TAS 10493827
GO:0015630 microtubule cytoskeleton IDA --
GO:0031514 motile cilium IEA --
genes like me logo Genes that share ontologies with SPAG6: view

Pathways & Interactions for SPAG6 Gene

SuperPathways for SPAG6 Gene

No Data Available

Gene Ontology (GO) - Biological Process for SPAG6 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0007286 spermatid development TAS 10493827
GO:0030030 cell projection organization IEA --
genes like me logo Genes that share ontologies with SPAG6: view

No data available for Pathways by source and SIGNOR curated interactions for SPAG6 Gene

Drugs & Compounds for SPAG6 Gene

No Compound Related Data Available

Transcripts for SPAG6 Gene

Unigene Clusters for SPAG6 Gene

Sperm associated antigen 6:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for SPAG6 Gene

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8a · 8b ^ 9 ^ 10a · 10b ^ 11
SP1: -
SP2: - -

Relevant External Links for SPAG6 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for SPAG6 Gene

mRNA expression in normal human tissues for SPAG6 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for SPAG6 Gene

This gene is overexpressed in Testis (x23.0), Pituitary (x6.1), and Brain - Hypothalamus (x4.3).

Protein differential expression in normal tissues from HIPED for SPAG6 Gene

This gene is overexpressed in Testis (26.0), Neutrophil (14.4), Ovary (11.5), Heart (10.7), and Plasma (6.5).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, and MOPED for SPAG6 Gene

SOURCE GeneReport for Unigene cluster for SPAG6 Gene Hs.655170

mRNA Expression by UniProt/SwissProt for SPAG6 Gene

Tissue specificity: Highly expressed in testis.
genes like me logo Genes that share expression patterns with SPAG6: view

Protein tissue co-expression partners for SPAG6 Gene

- Elite partner

Primer Products

In Situ Assay Products

Orthologs for SPAG6 Gene

This gene was present in the common ancestor of animals.

Orthologs for SPAG6 Gene

Organism Taxonomy Gene Similarity Type Details
(Bos Taurus)
Mammalia SPAG6 35
  • 91.94 (n)
  • 96.27 (a)
SPAG6 36
  • 96 (a)
(Canis familiaris)
Mammalia SPAG6 35
  • 92.93 (n)
  • 96.27 (a)
SPAG6 36
  • 93 (a)
(Mus musculus)
Mammalia Spag6 35
  • 87.57 (n)
  • 96.25 (a)
Spag6 16
BC061194 36
  • 92 (a)
(Pan troglodytes)
Mammalia SPAG6 35
  • 99.87 (n)
  • 100 (a)
SPAG6 36
  • 100 (a)
(Rattus norvegicus)
Mammalia Spag6 35
  • 87.42 (n)
  • 95.85 (a)
(Monodelphis domestica)
Mammalia SPAG6 36
  • 95 (a)
(Ornithorhynchus anatinus)
Mammalia SPAG6 36
  • 91 (a)
(Gallus gallus)
Aves SPAG6 35
  • 82.25 (n)
  • 85.8 (a)
SPAG6 36
  • 82 (a)
(Anolis carolinensis)
Reptilia SPAG6 36
  • 89 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia spag6 35
  • 77.65 (n)
  • 89.74 (a)
Str.9758 35
African clawed frog
(Xenopus laevis)
Amphibia Xl.16464 35
(Danio rerio)
Actinopterygii Dr.17566 35
spag6 35
  • 72.46 (n)
  • 83.99 (a)
spag6 36
  • 84 (a)
fruit fly
(Drosophila melanogaster)
Insecta alphaKap4 36
  • 17 (a)
(Caenorhabditis elegans)
Secernentea F53B2.5 36
  • 12 (a)
ima-1 36
  • 13 (a)
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.14201 35
sea squirt
(Ciona savignyi)
Ascidiacea CSA.8260 36
  • 82 (a)
Species with no ortholog for SPAG6:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for SPAG6 Gene

Gene Tree for SPAG6 (if available)
Gene Tree for SPAG6 (if available)

Paralogs for SPAG6 Gene

Paralogs for SPAG6 Gene

genes like me logo Genes that share paralogs with SPAG6: view

Variants for SPAG6 Gene

Sequence variations from dbSNP and Humsavar for SPAG6 Gene

SNP ID Clin Chr 10 pos Sequence Context AA Info Type MAF
rs1057263 -- 22,417,457(+) CCTAA(C/T)GTCAC utr-variant-3-prime
rs1170547 -- 22,378,250(+) gtaga(A/G)acggg intron-variant
rs1170548 -- 22,382,187(+) AAGAT(G/T)ACGCT intron-variant
rs1170549 -- 22,369,352(-) AGTGA(C/T)GGGGA intron-variant
rs1170550 -- 22,360,822(-) ATGCA(G/T)GGAAA reference, missense, intron-variant

Structural Variations from Database of Genomic Variants (DGV) for SPAG6 Gene

Variant ID Type Subtype PubMed ID
dgv136n67 CNV Gain 20364138
dgv137n67 CNV Gain 20364138
dgv624n71 CNV Loss 21882294
nsv6032 CNV Insertion 18451855

Variation tolerance for SPAG6 Gene

Residual Variation Intolerance Score: 27.69% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 6.44; 77.28% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for SPAG6 Gene

HapMap Linkage Disequilibrium report

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for SPAG6 Gene

Disorders for SPAG6 Gene

MalaCards: The human disease database

(1) MalaCards diseases for SPAG6 Gene - From: DISEASES and GeneCards

Disorder Aliases PubMed IDs
  • hydrocephalus, nonsyndromic, autosomal recessive
- elite association
Search SPAG6 in MalaCards View complete list of genes associated with diseases

Relevant External Links for SPAG6

Genetic Association Database (GAD)
Human Genome Epidemiology (HuGE) Navigator
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
genes like me logo Genes that share disorders with SPAG6: view

No data available for UniProtKB/Swiss-Prot and Genatlas for SPAG6 Gene

Publications for SPAG6 Gene

  1. cDNA cloning and characterization of a human sperm antigen (SPAG6) with homology to the product of the Chlamydomonas PF16 locus. (PMID: 10493827) Neilson L.I. … Strauss J.F. III (Genomics 1999) 2 23 67
  2. Inhibition of tyrosine phosphorylation of sperm flagellar proteins, outer dense fiber protein-2 and tektin-2, is associated with impaired motility during capacitation of hamster spermatozoa. (PMID: 19953638) Mariappa D. … Seshagiri P.B. (Mol. Reprod. Dev. 2010) 23 67
  3. Gene expression of Spag6 in chick central nervous system. (PMID: 20394600) Hamada T. … Asahara T. (Anat Histol Embryol 2010) 23 67
  4. A heterozygous mutation disrupting the SPAG16 gene results in biochemical instability of central apparatus components of the human sperm axoneme. (PMID: 17699735) Zhang Z. … Strauss J.F.III. (Biol. Reprod. 2007) 23 67
  5. Biochemical and immunochemical characterization of boar sperm flagellar protein with role in hyperactivation/capacitation process. (PMID: 16604153) Mollova M. … Kyurkchiev S. (Reprod Biol 2006) 23 67

Products for SPAG6 Gene

Sources for SPAG6 Gene

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