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Aliases for SPAG5 Gene

Aliases for SPAG5 Gene

  • Sperm Associated Antigen 5 2 3
  • Astrin 2 3 4 6
  • MAP126 3 4 6
  • Mitotic Spindle Coiled-Coil Related Protein 2 3
  • Mitotic Spindle Associated Protein P126 2 3
  • Mitotic Spindle-Associated Protein P126 3 4
  • DEEPEST 3 4
  • Sperm-Associated Antigen 5 3
  • HMAP126 3

External Ids for SPAG5 Gene

Previous GeneCards Identifiers for SPAG5 Gene

  • GC17M026749
  • GC17M028804
  • GC17M027050
  • GC17M023928
  • GC17M026904
  • GC17M023113

Summaries for SPAG5 Gene

Entrez Gene Summary for SPAG5 Gene

  • This gene encodes a protein associated with the mitotic spindle apparatus. The encoded protein may be involved in the functional and dynamic regulation of mitotic spindles. [provided by RefSeq, Jul 2008]

GeneCards Summary for SPAG5 Gene

SPAG5 (Sperm Associated Antigen 5) is a Protein Coding gene. Diseases associated with SPAG5 include patellar tendinitis and tendinitis. An important paralog of this gene is ENSG00000258472.

UniProtKB/Swiss-Prot for SPAG5 Gene

  • Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase. Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture. In complex with SKAP, promotes stable microtubule-kinetochore attachments. May contribute to the regulation of separase activity. May regulate AURKA localization to mitotic spindle, but not to centrosomes and CCNB1 localization to both mitotic spindle and centrosomes. In non-mitotic cells, upon stress induction, inhibits mammalian target of rapamycin complex 1 (mTORC1) association and recruits the mTORC1 component RPTOR to stress granules (SGs), thereby preventing mTORC1 hyperactivation-induced apoptosis. May enhance GSK3B-mediated phosphorylation of other substrates, such as MAPT/TAU.

Gene Wiki entry for SPAG5 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for SPAG5 Gene

Genomics for SPAG5 Gene

Regulatory Elements for SPAG5 Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for SPAG5 Gene

Start:
28,577,565 bp from pter
End:
28,599,279 bp from pter
Size:
21,715 bases
Orientation:
Minus strand

Genomic View for SPAG5 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for SPAG5 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for SPAG5 Gene

Proteins for SPAG5 Gene

  • Protein details for SPAG5 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q96R06-SPAG5_HUMAN
    Recommended name:
    Sperm-associated antigen 5
    Protein Accession:
    Q96R06
    Secondary Accessions:
    • O95213
    • Q9BWE8
    • Q9NT17
    • Q9UFE6

    Protein attributes for SPAG5 Gene

    Size:
    1193 amino acids
    Molecular mass:
    134422 Da
    Quaternary structure:
    • Homodimer, with a globular head domain and a long stalk. Homooligomer; the globular head domains associate, resulting in aster-like structures. Binds to microtubules in the mitotic spindle. Interacts with DCLRE1B/Apollo. Part of an astrin (SPAG5)-kinastrin (SKAP) complex containing KNSTRN, SPAG5, PLK1, DYNLL1 and SGOL2. Interacts with KNSTRN. Interacts with RPTOR; this interaction competes with RPTOR binding to MTOR, resulting in decreased mTORC1 formation. Interacts with G3BP1. The complex formed with G3BP1 AND RPTOR is increased by oxidative stress.
    SequenceCaution:
    • Sequence=AAD02813.1; Type=Frameshift; Positions=1093; Evidence={ECO:0000305}; Sequence=AAL06396.2; Type=Frameshift; Positions=1135; Evidence={ECO:0000305};

neXtProt entry for SPAG5 Gene

Proteomics data for SPAG5 Gene at MOPED

Post-translational modifications for SPAG5 Gene

Antibody Products

No data available for DME Specific Peptides for SPAG5 Gene

Domains for SPAG5 Gene

Protein Domains for SPAG5 Gene

InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for SPAG5 Gene

Graphical View of Domain Structure for InterPro Entry

genes like me logo Genes that share domains with SPAG5: view

No data available for Gene Families and UniProtKB/Swiss-Prot for SPAG5 Gene

Function for SPAG5 Gene

Molecular function for SPAG5 Gene

UniProtKB/Swiss-Prot Function:
Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase. Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture. In complex with SKAP, promotes stable microtubule-kinetochore attachments. May contribute to the regulation of separase activity. May regulate AURKA localization to mitotic spindle, but not to centrosomes and CCNB1 localization to both mitotic spindle and centrosomes. In non-mitotic cells, upon stress induction, inhibits mammalian target of rapamycin complex 1 (mTORC1) association and recruits the mTORC1 component RPTOR to stress granules (SGs), thereby preventing mTORC1 hyperactivation-induced apoptosis. May enhance GSK3B-mediated phosphorylation of other substrates, such as MAPT/TAU.
UniProtKB/Swiss-Prot Induction:
Expression is cell cycle-regulated, with an increase from prophase to cytokinesis and return to basal levels at the next G1 phase.

Gene Ontology (GO) - Molecular Function for SPAG5 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005515 protein binding IPI 19197158
genes like me logo Genes that share ontologies with SPAG5: view

Phenotypes for SPAG5 Gene

MGI mutant phenotypes for SPAG5:
inferred from 1 alleles
GenomeRNAi human phenotypes for SPAG5:
genes like me logo Genes that share phenotypes with SPAG5: view

Animal Models for SPAG5 Gene

MGI Knock Outs for SPAG5:

Animal Model Products

miRNA for SPAG5 Gene

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for SPAG5

In Situ Assay Products

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , Transcription Factor Targeting and HOMER Transcription for SPAG5 Gene

Localization for SPAG5 Gene

Subcellular locations from UniProtKB/Swiss-Prot for SPAG5 Gene

Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Chromosome, centromere, kinetochore. Midbody. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic granule. Note=In a punctate pattern in interphase cells. During mitosis, detected at spindle poles during prophase, throughout the spindle in metaphase and anaphase, and at midzone microtubules in anaphase and telophase. Efficient targeting to the mitotic spindle may depend upon phosphorylation by GSK3B. Detected on kinetochores of chromosomes that have congressed. The astrin (SPAG5)-kinastrin (SKAP) complex localizes to the microtubule plus ends (By similarity). In non-mitotic non-stressed cells, shows a microtubuli pattern. In arsenite-stressed cells, accumulates in stress granules. {ECO:0000250}.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for SPAG5 Gene COMPARTMENTS Subcellular localization image for SPAG5 gene
Compartment Confidence
cytoskeleton 5
nucleus 4
cytosol 3
mitochondrion 2
endoplasmic reticulum 1
peroxisome 1

Gene Ontology (GO) - Cellular Components for SPAG5 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000776 kinetochore IDA 21402792
GO:0000777 condensed chromosome kinetochore IEA --
GO:0000922 spindle pole IEA --
GO:0005634 nucleus IDA --
GO:0005654 nucleoplasm IDA --
genes like me logo Genes that share ontologies with SPAG5: view

Pathways for SPAG5 Gene

SuperPathways for SPAG5 Gene

No Data Available

Gene Ontology (GO) - Biological Process for SPAG5 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000070 mitotic sister chromatid segregation IMP 21402792
GO:0007051 spindle organization IMP 21402792
GO:0007059 chromosome segregation IMP 21402792
GO:0051301 cell division IEA --
GO:0051988 regulation of attachment of spindle microtubules to kinetochore IMP 21402792
genes like me logo Genes that share ontologies with SPAG5: view

No data available for Pathways by source for SPAG5 Gene

Transcripts for SPAG5 Gene

Unigene Clusters for SPAG5 Gene

Sperm associated antigen 5:
Representative Sequences:

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for SPAG5

Primer Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for SPAG5 Gene

ExUns: 1a · 1b · 1c ^ 2a · 2b ^ 3a · 3b ^ 4a · 4b ^ 5a · 5b ^ 6 ^ 7a · 7b ^ 8 ^ 9 ^ 10 ^ 11a · 11b ^ 12 ^ 13 ^ 14 ^ 15 ^ 16a · 16b ^ 17a ·
SP1: - -
SP2:
SP3: - - - -
SP4: -
SP5: - - - - - - - -
SP6:
SP7: -
SP8:
SP9: - - -

ExUns: 17b · 17c ^ 18 ^ 19 ^ 20a · 20b · 20c ^ 21 ^ 22 ^ 23 ^ 24 ^ 25
SP1:
SP2:
SP3:
SP4:
SP5:
SP6:
SP7:
SP8:
SP9:

Relevant External Links for SPAG5 Gene

GeneLoc Exon Structure for
SPAG5
ECgene alternative splicing isoforms for
SPAG5

Expression for SPAG5 Gene

mRNA expression in normal human tissues for SPAG5 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for SPAG5 Gene

This gene is overexpressed in Testis (6.5) and Kidney - Cortex (5.7).

Protein differential expression in normal tissues for SPAG5 Gene

This gene is overexpressed in Cervix (34.4), Plasma (14.6), Testis (11.4), and Bone (6.5).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for SPAG5 Gene

SOURCE GeneReport for Unigene cluster for SPAG5 Gene Hs.514033

mRNA Expression by UniProt/SwissProt for SPAG5 Gene

Q96R06-SPAG5_HUMAN
Tissue specificity: Highly expressed in testis. Detected at low levels in placenta, liver, pancreas, thymus and colon.
genes like me logo Genes that share expressions with SPAG5: view

Expression partners for SPAG5 Gene

In Situ Assay Products

Orthologs for SPAG5 Gene

This gene was present in the common ancestor of chordates.

Orthologs for SPAG5 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia SPAG5 35
  • 85.13 (n)
  • 78.4 (a)
SPAG5 36
  • 78 (a)
OneToOne
dog
(Canis familiaris)
Mammalia SPAG5 35
  • 86.21 (n)
  • 79.78 (a)
SPAG5 36
  • 79 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Spag5 35
  • 78.02 (n)
  • 68.11 (a)
Spag5 16
Spag5 36
  • 67 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Spag5 35
  • 78.08 (n)
  • 67.59 (a)
oppossum
(Monodelphis domestica)
Mammalia -- 36
  • 51 (a)
OneToMany
platypus
(Ornithorhynchus anatinus)
Mammalia -- 36
  • 53 (a)
ManyToMany
-- 36
  • 54 (a)
ManyToMany
chimpanzee
(Pan troglodytes)
Mammalia SPAG5 36
  • 98 (a)
OneToOne
chicken
(Gallus gallus)
Aves SPAG5 35
  • 48.6 (n)
  • 37.08 (a)
-- 36
  • 26 (a)
OneToMany
zebrafish
(Danio rerio)
Actinopterygii SPAG5 36
  • 14 (a)
OneToMany
Species with no ortholog for SPAG5:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • lizard (Anolis carolinensis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for SPAG5 Gene

ENSEMBL:
Gene Tree for SPAG5 (if available)
TreeFam:
Gene Tree for SPAG5 (if available)

Paralogs for SPAG5 Gene

Paralogs for SPAG5 Gene

genes like me logo Genes that share paralogs with SPAG5: view

Variants for SPAG5 Gene

Sequence variations from dbSNP and Humsavar for SPAG5 Gene

SNP ID Clin Chr 17 pos Sequence Context AA Info Type MAF
rs496622 -- 28,595,996(-) catct(A/G)gggtc intron-variant
rs723267 -- 28,580,648(+) ttcca(A/G)gacat intron-variant
rs1008254 -- 28,582,945(-) ACCTT(C/T)TTTGA intron-variant
rs2302206 -- 28,585,678(-) CTGTG(G/T)CCTTG intron-variant
rs3132814 -- 28,581,855(-) CCTGT(G/T)TGGCA intron-variant

Relevant External Links for SPAG5 Gene

HapMap Linkage Disequilibrium report
SPAG5

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Structural Variations from Database of Genomic Variants (DGV) for SPAG5 Gene

Disorders for SPAG5 Gene

MalaCards: The human disease database

MalaCards: The human disease database.

Search for SPAG5 Gene in MalaCards »

(2) Diseases for SPAG5 Gene including...

(1) University of Copenhagen DISEASES for SPAG5 Gene

Relevant External Links for SPAG5

Genetic Association Database (GAD)
SPAG5
Human Genome Epidemiology (HuGE) Navigator
SPAG5
genes like me logo Genes that share disorders with SPAG5: view

No data available for OMIM , UniProtKB/Swiss-Prot , Novoseek inferred disease relationships and Genatlas for SPAG5 Gene

Publications for SPAG5 Gene

  1. Analysis of mitotic microtubule-associated proteins using mass spectrometry identifies astrin, a spindle-associated protein. (PMID: 11724960) Mack G.J. … Compton D.A. (Proc. Natl. Acad. Sci. U.S.A. 2001) 3 4 23
  2. The mitotic-spindle-associated protein astrin is essential for progression through mitosis. (PMID: 12356910) Gruber J. … Hatzfeld M. (J. Cell Sci. 2002) 3 4 23
  3. Astrin is required for the maintenance of sister chromatid cohesion and centrosome integrity. (PMID: 17664331) Thein K.H. … Gruneberg U. (J. Cell Biol. 2007) 3 4 23
  4. Glycogen synthase kinase 3beta interacts with and phosphorylates the spindle-associated protein astrin. (PMID: 18055457) Cheng T.S. … Hong Y.R. (J. Biol. Chem. 2008) 3 4 23
  5. Astrin regulates Aurora-A localization. (PMID: 18361916) Du J. … Hannon G.J. (Biochem. Biophys. Res. Commun. 2008) 3 4 23

Products for SPAG5 Gene

  • Addgene plasmids for SPAG5

Sources for SPAG5 Gene

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