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Aliases for SPAG4 Gene

Aliases for SPAG4 Gene

  • Sperm Associated Antigen 4 2 3
  • Outer Dense Fiber-Associated Protein SPAG4 3 4
  • Sad1 And UNC84 Domain Containing 4 2 3
  • SUN Domain-Containing Protein 4 3 4
  • Cancer/Testis Antigen 127 2 3
  • Acrosomal Protein ACR55 2 3
  • SUN4 3 4
  • Sperm-Associated Antigen 4 Protein 3
  • Sperm Tail Protein 3
  • CT127 3

External Ids for SPAG4 Gene

Previous GeneCards Identifiers for SPAG4 Gene

  • GC20P033962
  • GC20P034872
  • GC20P034919
  • GC20P033667
  • GC20P034203
  • GC20P030981

Summaries for SPAG4 Gene

Entrez Gene Summary for SPAG4 Gene

  • The mammalian sperm flagellum contains two cytoskeletal structures associated with the axoneme: the outer dense fibers surrounding the axoneme in the midpiece and principal piece and the fibrous sheath surrounding the outer dense fibers in the principal piece of the tail. Defects in these structures are associated with abnormal tail morphology, reduced sperm motility, and infertility. In the rat, the protein encoded by this gene associates with an outer dense fiber protein via a leucine zipper motif and localizes to the microtubules of the manchette and axoneme during sperm tail development. [provided by RefSeq, Jul 2008]

GeneCards Summary for SPAG4 Gene

SPAG4 (Sperm Associated Antigen 4) is a Protein Coding gene. GO annotations related to this gene include structural molecule activity. An important paralog of this gene is SUN5.

UniProtKB/Swiss-Prot for SPAG4 Gene

  • May assist the organization and assembly of outer dense fibers (ODFs), a specific structure of the sperm tail

No data available for Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for SPAG4 Gene

Genomics for SPAG4 Gene

Regulatory Elements for SPAG4 Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for SPAG4 Gene

35,615,854 bp from pter
35,621,049 bp from pter
5,196 bases
Plus strand

Genomic View for SPAG4 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for SPAG4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for SPAG4 Gene

Proteins for SPAG4 Gene

  • Protein details for SPAG4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Sperm-associated antigen 4 protein
    Protein Accession:
    Secondary Accessions:
    • O43648

    Protein attributes for SPAG4 Gene

    437 amino acids
    Molecular mass:
    48165 Da
    Quaternary structure:
    • Homodimer. Interacts with ODF1. May associate with microtubules (By similarity).
    • Sequence=AAC32052.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};

neXtProt entry for SPAG4 Gene

Proteomics data for SPAG4 Gene at MOPED

Post-translational modifications for SPAG4 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for SPAG4 Gene

ENSEMBL proteins:
REFSEQ proteins:

Antibody Products

No data available for DME Specific Peptides for SPAG4 Gene

Domains for SPAG4 Gene

Protein Domains for SPAG4 Gene


Suggested Antigen Peptide Sequences for SPAG4 Gene

Graphical View of Domain Structure for InterPro Entry



  • Contains 1 SUN domain.
  • Contains 1 SUN domain.
genes like me logo Genes that share domains with SPAG4: view

No data available for Gene Families for SPAG4 Gene

Function for SPAG4 Gene

Molecular function for SPAG4 Gene

GENATLAS Biochemistry:
spermatid-specific gene 4 interacting specifically with the 27kDa outer dense fiber protein ODF27 in elongation spermatid
UniProtKB/Swiss-Prot Function:
May assist the organization and assembly of outer dense fibers (ODFs), a specific structure of the sperm tail

Gene Ontology (GO) - Molecular Function for SPAG4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005198 structural molecule activity TAS 9691178
genes like me logo Genes that share ontologies with SPAG4: view

Animal Model Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for SPAG4

In Situ Assay Products

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , Phenotypes , Animal Models , miRNA , Transcription Factor Targets and HOMER Transcription for SPAG4 Gene

Localization for SPAG4 Gene

Subcellular locations from UniProtKB/Swiss-Prot for SPAG4 Gene

Membrane; Multi-pass membrane protein. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, flagellum axoneme. Note=In spermatids, it is localized in the transient manchette and in the axoneme of elongating spermatids and epididymal sperm. {ECO:0000250}.

Subcellular locations from

Jensen Localization Image for SPAG4 Gene COMPARTMENTS Subcellular localization image for SPAG4 gene
Compartment Confidence
cytoskeleton 5
plasma membrane 3
cytosol 1
peroxisome 1

Gene Ontology (GO) - Cellular Components for SPAG4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005635 nuclear envelope IBA --
GO:0005737 cytoplasm IEA --
GO:0005856 cytoskeleton IEA --
GO:0016021 integral component of membrane IEA --
GO:0031514 motile cilium IEA --
genes like me logo Genes that share ontologies with SPAG4: view

Pathways for SPAG4 Gene

SuperPathways for SPAG4 Gene

No Data Available

Gene Ontology (GO) - Biological Process for SPAG4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006998 nuclear envelope organization IBA --
GO:0007283 spermatogenesis TAS 9691178
GO:0090286 cytoskeletal anchoring at nuclear membrane IBA --
genes like me logo Genes that share ontologies with SPAG4: view

No data available for Pathways by source for SPAG4 Gene

Drugs for SPAG4 Gene

(3) Novoseek inferred chemical compound relationships for SPAG4 Gene

Compound -log(P) Hits PubMed IDs
pentoxifylline 55.4 2
leucine 50.9 4
tyrosine 19 1
genes like me logo Genes that share compounds with SPAG4: view

Transcripts for SPAG4 Gene

Unigene Clusters for SPAG4 Gene

Sperm associated antigen 4:
Representative Sequences:

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for SPAG4

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for SPAG4 Gene

ExUns: 1 ^ 2a · 2b · 2c · 2d · 2e ^ 3 ^ 4a · 4b ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11a · 11b · 11c ^ 12 ^ 13
SP1: - - - - -
SP2: - - -
SP3: - -
SP4: -

Relevant External Links for SPAG4 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for SPAG4 Gene

mRNA expression in normal human tissues for SPAG4 Gene

mRNA differential expression in normal tissues according to GTEx for SPAG4 Gene

This gene is overexpressed in Testis (8.3) and Pancreas (7.9).

Protein differential expression in normal tissues for SPAG4 Gene

This gene is overexpressed in Cervix (65.4).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, and MOPED for SPAG4 Gene

SOURCE GeneReport for Unigene cluster for SPAG4 Gene Hs.123159

genes like me logo Genes that share expressions with SPAG4: view

Expression partners for SPAG4 Gene

* - Elite partner

Primer Products

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery and mRNA Expression by UniProt/SwissProt for SPAG4 Gene

Orthologs for SPAG4 Gene

This gene was present in the common ancestor of animals.

Orthologs for SPAG4 Gene

Organism Taxonomy Gene Similarity Type Details
(Bos Taurus)
Mammalia SPAG4 35
  • 88.01 (n)
  • 87.15 (a)
SPAG4 36
  • 87 (a)
(Canis familiaris)
Mammalia SPAG4 35
  • 87.72 (n)
  • 87.64 (a)
SPAG4 36
  • 74 (a)
(Mus musculus)
Mammalia Spag4 35
  • 84.56 (n)
  • 82.66 (a)
Spag4 16
Spag4 36
  • 81 (a)
(Pan troglodytes)
Mammalia SPAG4 35
  • 99.85 (n)
  • 100 (a)
SPAG4 36
  • 100 (a)
(Rattus norvegicus)
Mammalia LOC100911109 35
  • 85.35 (n)
  • 83.14 (a)
(Monodelphis domestica)
Mammalia SPAG4 36
  • 59 (a)
(Gallus gallus)
Aves -- 36
  • 37 (a)
-- 36
  • 39 (a)
-- 36
  • 39 (a)
-- 36
  • 32 (a)
-- 36
  • 33 (a)
-- 36
  • 39 (a)
-- 36
  • 31 (a)
-- 36
  • 41 (a)
-- 36
  • 37 (a)
(Anolis carolinensis)
Reptilia -- 36
  • 37 (a)
fruit fly
(Drosophila melanogaster)
Insecta koi 36
  • 16 (a)
(Caenorhabditis elegans)
Secernentea unc-84 36
  • 8 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 25 (a)
Species with no ortholog for SPAG4:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)
  • zebrafish (Danio rerio)

Evolution for SPAG4 Gene

Gene Tree for SPAG4 (if available)
Gene Tree for SPAG4 (if available)

Paralogs for SPAG4 Gene

Paralogs for SPAG4 Gene

(5) SIMAP similar genes for SPAG4 Gene using alignment to 3 proteins:

genes like me logo Genes that share paralogs with SPAG4: view

Variants for SPAG4 Gene

Sequence variations from dbSNP and Humsavar for SPAG4 Gene

SNP ID Clin Chr 20 pos Sequence Context AA Info Type MAF
rs761827 -- 35,619,975(+) TTTTG(C/T)TGTTG intron-variant
rs2425067 -- 35,620,594(+) ATACA(C/T)AGAAG intron-variant
rs3841688 -- 35,617,467(+) CTGAG(-/C)CCCCG intron-variant
rs6058279 -- 35,614,522(+) ttagc(C/T)gggtg upstream-variant-2KB
rs6058280 -- 35,620,599(+) CAGAA(A/G)ATCCT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for SPAG4 Gene

Variant ID Type Subtype PubMed ID
nsv526188 CNV Gain 19592680

Relevant External Links for SPAG4 Gene

HapMap Linkage Disequilibrium report

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for SPAG4 Gene

Disorders for SPAG4 Gene

No disorders were found for SPAG4 Gene.

No data available for MalaCards , OMIM , UniProtKB/Swiss-Prot , University of Copenhagen DISEASES , Novoseek inferred disease relationships , Genatlas and External Links for SPAG4 Gene

Publications for SPAG4 Gene

  1. A novel testis-specific gene, SPAG4, whose product interacts specifically with outer dense fiber protein ODF27, maps to human chromosome 20q11.2. (PMID: 9691178) Tarnasky H. … van der Hoorn F.A. (Cytogenet. Cell Genet. 1998) 2 3 4
  2. Human sperm associated antigen 4 (SPAG4) is a potential cancer marker. (PMID: 14614621) Kennedy C. … O'Bryan M.K. (Cell Tissue Res. 2004) 3 4 23
  3. Spag4, a novel sperm protein, binds outer dense-fiber protein Odf1 and localizes to microtubules of manchette and axoneme. (PMID: 10373309) Shao X. … van der Hoorn F.A. (Dev. Biol. 1999) 2 3 23
  4. The DNA sequence and comparative analysis of human chromosome 20. (PMID: 11780052) Deloukas P. … Rogers J. (Nature 2001) 3 4
  5. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PMID: 12477932) Strausberg R.L. … Marra M.A. (Proc. Natl. Acad. Sci. U.S.A. 2002) 3

Products for SPAG4 Gene

Sources for SPAG4 Gene

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