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Aliases for SPAG16 Gene

Aliases for SPAG16 Gene

  • Sperm Associated Antigen 16 2 3
  • PF20 3 4 6
  • Pf20 Protein Homolog 3 4
  • Sperm-Associated Antigen 16 Protein 3
  • Sperm-Associated WD Repeat Protein 3
  • WD Repeat Domain 29 3
  • WDR29 3

External Ids for SPAG16 Gene

Previous GeneCards Identifiers for SPAG16 Gene

  • GC02P213974
  • GC02P213857
  • GC02P214149
  • GC02P206000

Summaries for SPAG16 Gene

Entrez Gene Summary for SPAG16 Gene

  • Cilia and flagella are comprised of a microtubular backbone, the axoneme, which is organized by the basal body and surrounded by plasma membrane. SPAG16 encodes 2 major proteins that associate with the axoneme of sperm tail and the nucleus of postmeiotic germ cells, respectively (Zhang et al., 2007 [PubMed 17699735]).[supplied by OMIM, Jul 2008]

GeneCards Summary for SPAG16 Gene

SPAG16 (Sperm Associated Antigen 16) is a Protein Coding gene. GO annotations related to this gene include protein kinase binding. An important paralog of this gene is PRPF4.

UniProtKB/Swiss-Prot for SPAG16 Gene

  • Necessary for sperm flagellar function. Plays a role in motile ciliogenesis. May help to recruit STK36 to the cilium or apical surface of the cell to initiate subsequent steps of construction of the central pair apparatus of motile cilia (By similarity).

No data available for Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for SPAG16 Gene

Genomics for SPAG16 Gene

Regulatory Elements for SPAG16 Gene

Genomic Location for SPAG16 Gene

213,284,379 bp from pter
214,410,501 bp from pter
1,126,123 bases
Plus strand

Genomic View for SPAG16 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for SPAG16 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for SPAG16 Gene

Proteins for SPAG16 Gene

  • Protein details for SPAG16 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Sperm-associated antigen 16 protein
    Protein Accession:
    Secondary Accessions:
    • Q498B7
    • Q658W1
    • Q68DB3
    • Q6I9Z6
    • Q8N9C7
    • Q9H601

    Protein attributes for SPAG16 Gene

    631 amino acids
    Molecular mass:
    70818 Da
    Quaternary structure:
    • Interacts with SPAG6 and STK36.

    Alternative splice isoforms for SPAG16 Gene


neXtProt entry for SPAG16 Gene

Proteomics data for SPAG16 Gene at MOPED

Post-translational modifications for SPAG16 Gene

Antibody Products

No data available for DME Specific Peptides for SPAG16 Gene

Domains for SPAG16 Gene

Gene Families for SPAG16 Gene

  • WDR :WD repeat domain containing

Suggested Antigen Peptide Sequences for SPAG16 Gene

Graphical View of Domain Structure for InterPro Entry



  • Q8N0X2
  • Contains 7 WD repeats.
genes like me logo Genes that share domains with SPAG16: view

Function for SPAG16 Gene

Molecular function for SPAG16 Gene

UniProtKB/Swiss-Prot Function:
Necessary for sperm flagellar function. Plays a role in motile ciliogenesis. May help to recruit STK36 to the cilium or apical surface of the cell to initiate subsequent steps of construction of the central pair apparatus of motile cilia (By similarity).

Gene Ontology (GO) - Molecular Function for SPAG16 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005515 protein binding --
GO:0019901 protein kinase binding IEA --
genes like me logo Genes that share ontologies with SPAG16: view
genes like me logo Genes that share phenotypes with SPAG16: view

Animal Models for SPAG16 Gene

MGI Knock Outs for SPAG16:

Animal Model Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for SPAG16

In Situ Assay Products

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , miRNA , Transcription Factor Targeting and HOMER Transcription for SPAG16 Gene

Localization for SPAG16 Gene

Subcellular locations from UniProtKB/Swiss-Prot for SPAG16 Gene

Cytoplasm. Cytoplasm, cytoskeleton, flagellum axoneme. Cytoplasm, cytoskeleton, cilium axoneme. Note=Detected on the sperm flagellum axoneme. Detected in the central apparatus of the axoneme. Colocalizes with SPAG6 on microtubules (By similarity). {ECO:0000250}.

Subcellular locations from

Jensen Localization Image for SPAG16 Gene COMPARTMENTS Subcellular localization image for SPAG16 gene
Compartment Confidence
cytoskeleton 2
mitochondrion 2
nucleus 2
cytosol 1
plasma membrane 1

Gene Ontology (GO) - Cellular Components for SPAG16 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IEA --
GO:0005737 cytoplasm --
GO:0005930 axoneme ISS --
GO:0015630 microtubule cytoskeleton IEA --
GO:0031514 motile cilium IEA --
genes like me logo Genes that share ontologies with SPAG16: view

Pathways for SPAG16 Gene

SuperPathways for SPAG16 Gene

No Data Available

Interacting Proteins for SPAG16 Gene

Gene Ontology (GO) - Biological Process for SPAG16 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0007283 spermatogenesis IEA --
GO:0035082 axoneme assembly IMP 17699735
GO:0042384 cilium assembly ISS --
genes like me logo Genes that share ontologies with SPAG16: view

No data available for Pathways by source for SPAG16 Gene

Transcripts for SPAG16 Gene

Unigene Clusters for SPAG16 Gene

Sperm associated antigen 16:
Representative Sequences:

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for SPAG16

Primer Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for SPAG16 Gene

ExUns: 1a · 1b · 1c ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6 ^ 7 ^ 8
SP1: - -
SP2: -

Relevant External Links for SPAG16 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for SPAG16 Gene

mRNA expression in normal human tissues for SPAG16 Gene

Protein differential expression in normal tissues for SPAG16 Gene

This gene is overexpressed in Bone marrow mesenchymal stem cell (59.7) and Testis (9.3).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, and MOPED for SPAG16 Gene

SOURCE GeneReport for Unigene cluster for SPAG16 Gene Hs.743365

mRNA Expression by UniProt/SwissProt for SPAG16 Gene

Tissue specificity: Isoform 1 is detected in testis. Isoform 4 is detected in testis and brain, and at lower levels in kidney, heart, pancreas, thyroid, ovary, adrenal gland, spinal cord, trachea and liver.
genes like me logo Genes that share expressions with SPAG16: view

Expression partners for SPAG16 Gene

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery and mRNA differential expression in normal tissues for SPAG16 Gene

Orthologs for SPAG16 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for SPAG16 Gene

Organism Taxonomy Gene Similarity Type Details
(Bos Taurus)
Mammalia SPAG16 35
  • 87.95 (n)
  • 85.51 (a)
SPAG16 36
  • 82 (a)
(Canis familiaris)
Mammalia SPAG16 35
  • 88.96 (n)
  • 86.78 (a)
-- 36
  • 81 (a)
-- 36
  • 88 (a)
(Mus musculus)
Mammalia Spag16 35
  • 79.86 (n)
  • 76.44 (a)
Spag16 16
Spag16 36
  • 74 (a)
(Pan troglodytes)
Mammalia SPAG16 35
  • 99.15 (n)
  • 99.05 (a)
SPAG16 36
  • 99 (a)
(Rattus norvegicus)
Mammalia Spag16 35
  • 81.66 (n)
  • 79.94 (a)
(Monodelphis domestica)
Mammalia SPAG16 36
  • 65 (a)
(Ornithorhynchus anatinus)
Mammalia -- 36
  • 74 (a)
-- 36
  • 55 (a)
(Gallus gallus)
Aves SPAG16 35
  • 64.83 (n)
  • 55.26 (a)
SPAG16 36
  • 51 (a)
(Anolis carolinensis)
Reptilia SPAG16 36
  • 55 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia spag16 35
  • 61.02 (n)
  • 57.46 (a)
Str.13914 35
African clawed frog
(Xenopus laevis)
Amphibia Xl.16242 35
(Danio rerio)
Actinopterygii LOC100536264 35
  • 56.69 (n)
  • 49.66 (a)
SPAG16 36
  • 47 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes PRP4 36
  • 20 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 45 (a)
Species with no ortholog for SPAG16:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for SPAG16 Gene

Gene Tree for SPAG16 (if available)
Gene Tree for SPAG16 (if available)

Paralogs for SPAG16 Gene

Paralogs for SPAG16 Gene Pseudogenes for SPAG16 Gene

genes like me logo Genes that share paralogs with SPAG16: view

Variants for SPAG16 Gene

Sequence variations from dbSNP and Humsavar for SPAG16 Gene

SNP ID Clin Chr 02 pos Sequence Context AA Info Type MAF
rs339808 -- 213,425,003(-) GAGTA(A/T)ATCCC intron-variant
rs339809 -- 213,411,043(+) tatca(A/G)tcagt intron-variant
rs339810 -- 213,413,897(+) TATTT(C/T)ACAGT intron-variant
rs339811 -- 213,418,905(+) CATCA(A/G)CTATA intron-variant
rs339812 -- 213,419,689(+) GAAAA(G/T)AATTC intron-variant

Structural Variations from Database of Genomic Variants (DGV) for SPAG16 Gene

Variant ID Type Subtype PubMed ID
nsv834531 CNV Loss 17160897
nsv875820 CNV Loss 21882294
nsv3152 CNV Insertion 18451855
nsv508884 CNV Insertion 20534489
esv1010545 CNV Insertion 20482838
esv1335096 CNV Insertion 17803354
dgv4431n71 CNV Loss 21882294
nsv428406 CNV Gain+Loss 18775914
nsv834532 CNV Gain 17160897
nsv523669 CNV Loss 19592680
nsv520985 CNV Loss 19592680
esv2721432 CNV Deletion 23290073
nsv834533 CNV Gain 17160897
esv2670902 CNV Deletion 23128226
esv2721433 CNV Deletion 23290073
esv2661130 CNV Deletion 23128226
esv2666545 CNV Deletion 23128226
esv2668503 CNV Deletion 23128226
nsv511092 CNV Complex 20534489
esv4596 CNV Deletion 18987735
esv2238037 CNV Deletion 18987734
esv2721434 CNV Deletion 23290073
esv1286091 CNV Deletion 17803354
esv267963 CNV Insertion 20981092
nsv527687 CNV Loss 19592680
esv2674107 CNV Deletion 23128226
nsv214963 CNV Loss 16902084
nsv528970 CNV Loss 19592680
nsv875823 CNV Loss 21882294
nsv875824 CNV Loss 21882294
esv2581361 CNV Deletion 19546169
esv26572 CNV Loss 19812545
nsv213247 CNV Insertion 16902084
esv2721435 CNV Deletion 23290073
esv1440695 CNV Insertion 17803354
esv1770635 CNV Insertion 17803354
dgv4432n71 CNV Loss 21882294
nsv875826 CNV Gain 21882294
nsv875827 CNV Loss 21882294
esv269956 CNV Insertion 20981092
esv2671825 CNV Deletion 23128226
nsv508127 CNV Loss 20534489
esv2421051 CNV Deletion 18987734
esv2721436 CNV Deletion 23290073
esv6741 CNV Loss 19470904
nsv214436 CNV Loss 16902084
esv2742312 CNV Deletion 23290073
esv2721437 CNV Deletion 23290073
esv2202966 CNV Deletion 18987734
esv1486943 CNV Deletion 17803354
esv995716 CNV Deletion 20482838
nsv213260 CNV Loss 16902084
nsv3153 CNV Insertion 18451855
esv2721438 CNV Deletion 23290073
esv2666218 CNV Deletion 23128226
esv2474779 CNV Deletion 19546169
esv2144910 CNV Deletion 18987734
esv268657 CNV Insertion 20981092
nsv821894 CNV Loss 20364138
esv2677096 CNV Deletion 23128226
esv28344 CNV Loss 19812545
nsv215392 CNV Loss 16902084
esv2721440 CNV Deletion 23290073
esv2663252 CNV Deletion 23128226
nsv834534 CNV Loss 17160897
esv2721441 CNV Deletion 23290073
esv2666614 CNV Deletion 23128226
esv2006955 CNV Deletion 18987734
esv2676709 CNV Deletion 23128226
nsv3155 CNV Loss 18451855
nsv875829 CNV Gain 21882294
nsv519662 CNV Gain 19592680
esv2529046 CNV Deletion 19546169
esv267863 CNV Insertion 20981092
esv272296 CNV Insertion 20981092
esv1554289 CNV Insertion 17803354
nsv508128 CNV Loss 20534489

Relevant External Links for SPAG16 Gene

HapMap Linkage Disequilibrium report
Human Gene Mutation Database (HGMD)

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for SPAG16 Gene

Disorders for SPAG16 Gene

Relevant External Links for SPAG16

Genetic Association Database (GAD)
Human Genome Epidemiology (HuGE) Navigator

No disorders were found for SPAG16 Gene.

No data available for MalaCards , OMIM , UniProtKB/Swiss-Prot , University of Copenhagen DISEASES , Novoseek inferred disease relationships and Genatlas for SPAG16 Gene

Publications for SPAG16 Gene

  1. Isolation and expression of the human hPF20 gene orthologous to Chlamydomonas PF20: evaluation as a candidate for axonemal defects of respiratory cilia and sperm flagella. (PMID: 11867345) Pennarun G. … Duriez B. (Am. J. Respir. Cell Mol. Biol. 2002) 2 3 4
  2. A sperm-associated WD repeat protein orthologous to Chlamydomonas PF20 associates with Spag6, the mammalian orthologue of Chlamydomonas PF16. (PMID: 12391165) Zhang Z. … Strauss J.F. III (Mol. Cell. Biol. 2002) 2 3 4
  3. A heterozygous mutation disrupting the SPAG16 gene results in biochemical instability of central apparatus components of the human sperm axoneme. (PMID: 17699735) Zhang Z. … Strauss J.F.III. (Biol. Reprod. 2007) 3 23
  4. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T. … Sugano S. (Nat. Genet. 2004) 3 4
  5. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard D.S. … Malek J. (Genome Res. 2004) 3 4

Products for SPAG16 Gene

Sources for SPAG16 Gene

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