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Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

SP1 Gene

protein-coding GIFtS: 66
GCID: GC12P053773

Sp1 Transcription Factor

Microbiology & Infectious Diseases Congress

123 related diseases
at

MalaCards

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, ¹⁰fRNAdb, ¹²H-InvDB, ¹³NCBI, ¹⁴NONCODE, and/or ¹⁵RNAdb)
About This Section

Aliases
Sp1 Transcription Factor¹ ²
Specificity Protein 1¹ ²
Transcription Factor Sp1²
TSFP1³

External Ids:	HGNC: 11205¹	Entrez Gene: 6667²	Ensembl: ENSG00000185591⁷	OMIM: 189906⁵	UniProtKB: P08047³

Export aliases for SP1 gene to outside databases

Previous GC identifers: GC12P053801 GC12P053490 GC12P052060 GC12P050814

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for SP1 Gene:

The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many

promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell

growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational

modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly

affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding

different isoforms have been found for this gene. (provided by RefSeq, Oct 2011)

GeneCards Summary for SP1 Gene:

SP1 (Sp1 transcription factor) is a protein-coding gene. Diseases associated with SP1 include cerebrotendinous xanthomatosis, and erythropoietic protoporphyria, and among its related super-pathways are Activation of Gene Expression by SREBP (SREBF) and Prolactin Signaling Pathway. GO annotations related to this gene include sequence-specific DNA binding and sequence-specific DNA binding transcription factor activity. An important paralog of this gene is KLF1.

UniProtKB/Swiss-Prot: SP1_HUMAN, P08047

Function: Transcription factor that can activate or repress transcription in response to physiological and

pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number

of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses.

Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage,

glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the

cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of

SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression. In complex

with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression

Gene Wiki entry for SP1 (Sp1 transcription factor) Gene

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 73), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000012.11 NC_018923.2 NT_029419.12

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the SP1 gene promoter:
STAT1 AP-1 ATF-2 c-Jun
Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: SP1 promoter sequence

Search SABiosciences Chromatin IP Primers for SP1

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat SP1

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 12q13.1 Ensembl cytogenetic band: 12q13.13 HGNC cytogenetic band: 12q13.1

SP1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
SP1 gene location

GeneLoc information about chromosome 12 GeneLoc Exon Structure

GeneLoc location for GC12P053773: view genomic region (about GC identifiers)

Start:	53,773,960 bp from pter	End:	53,810,230 bp from pter
Size:	36,271 bases	Orientation:	plus strand

(According to UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE ¹MOPED, ²PaxDb, and ³MAXQB RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Ontologies according to Gene Ontology Consortium 01 Oct 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
About This Section

UniProtKB/Swiss-Prot: SP1_HUMAN, P08047 (See protein sequence)

Recommended Name: Transcription factor Sp1

Size: 785 amino acids; 80693 Da

Subunit: Interacts with ATF7IP, ATF7IP2, BAHD1, POGZ, HCFC1, AATF and PHC2. Interacts with varicella-zoster virus

IE62 protein. Interacts with HIV-1 Vpr; the interaction is inhibited by SP1 O-glycosylation. Interacts with SV40

VP2/3 proteins. Interacts with SV40 major capsid protein VP1; this interaction leads to a cooperativity between

the 2 proteins in DNA binding. Interacts with HLTF; the interaction may be required for basal transcriptional

activity of HLTF. Interacts (deacetylated form) with EP300; the interaction enhances gene expression. Interacts

with HDAC1 and JUN. Interacts with ELF1; the interaction is inhibited by glycosylation of SP1. Interaction with

NFYA; the interaction is inhibited by glycosylation of SP1. Interacts with SMARCA4/BRG1 (By similarity).

Interacts with ATF7IP and TBP. Interacts with MEIS2 isoform 4 and PBX1 isoform PBX1a. Interacts with EGR1

Subcellular location: Nucleus. Cytoplasm. Note=Nuclear location is governed by glycosylated/phosphorylated states.

Insulin promotes nuclear location, while glucagon favors cytoplasmic location

Miscellaneous: In the hepatoma cell line Hep-G2, SP1 precursor mRNA may undergo homotype trans-splicing leading to

the duplication of exons 2 and 3

Sequence caution: Sequence=AAH43224.1; Type=Erroneous initiation;

5 PDB 3D structures from and Proteopedia for SP1:

1SP1 (3D)

1SP2 (3D)

1VA1 (3D)

1VA2 (3D)

1VA3 (3D)

Secondary accessions: Q86TN8 Q9H3Q5 Q9NR51 Q9NY21 Q9NYE7

Explore the universe of human proteins at neXtProt for SP1: NX_P08047

Explore proteomics data for SP1 at MOPED

Post-translational modifications:

UniProtKB: Phosphorylated on multiple serine and threonine residues. Phosphorylation is coupled to ubiquitination,

sumoylation and proteolytic processing. Phosphorylation on Ser-59 enhances proteolytic cleavage. Phosphorylation

on Ser-7 enhances ubiquitination and protein degradation. Hyperphosphorylation on Ser-101 in response to DNA

damage has no effect on transcriptional activity. MAPK1/MAPK3-mediated phosphorylation on Thr-453 and Thr-739

enhances VEGF transcription but, represses FGF2-triggered PDGFR-alpha transcription. Also implicated in the

repression of RECK by ERBB2. Hyperphosphorylated on Thr-278 and Thr-739 during mitosis by MAPK8 shielding SP1

from degradation by the ubiquitin-dependent pathway. Phosphorylated in the zinc-finger domain by

calmodulin-activated PKCzeta. Phosphorylation on Ser-641 by PKCzeta is critical for TSA-activated LHR gene

expression through release of its repressor, p107. Phosphorylation on Thr-668, Ser-670 and Thr-681 is stimulated

by angiotensin II via the AT1 receptor inducing increased binding to the PDGF-D promoter. This phosphorylation is

increased in injured artey wall. Ser-59 and Thr-681 can both be dephosphorylated by PP2A during cell-cycle

interphase. Dephosphorylation on Ser-59 leads to increased chromatin association during interphase and increases

the transcriptional activity. On insulin stimulation, sequentially glycosylated and phosphorylated on several

C-terminal serine and threonine residues

UniProtKB: Acetylated. Acetylation/deacetylation events affect transcriptional activity. Deacetylation leads to an increase

in the expression the 12(s)-lipooxygenase gene though recruitment of p300 to the promoter

UniProtKB: Ubiquitinated. Ubiquitination occurs on the C-terminal proteolytically-cleaved peptide and is triggered by

phosphorylation

UniProtKB: Sumoylated with SUMO1. Sumoylation modulates proteolytic cleavage of the N-terminal repressor domain. Sumoylation

levels are attenuated during tumorigenesis. Phosphorylation mediates SP1 desumoylation

UniProtKB: Proteolytic cleavage in the N-terminal repressor domain is prevented by sumoylation. The C-terminal cleaved

product is susceptible to degradation

UniProtKB: O-glycosylated; Contains 8 N-acetylglucosamine side chains. Levels are controlled by insulin and the SP1

phosphorylation states. Insulin-mediated O-glycosylation locates SP1 to the nucleus, where it is sequentially

deglycosylated and phosphorylated. O-glycosylation affects transcriptional activity through disrupting the

interaction with a number of transcription factors including ELF1 and NFYA. Also inhibits interaction with the

HIV1 promoter. Inhibited by peroxisomome proliferator receptor gamma (PPARgamma)

View modification sites using PhosphoSitePlus

View neXtProt modification sites for NX_P08047

SP1 Protein expression data from MOPED¹, PaxDb² and MAXQB³ : About this image

REFSEQ proteins (3 alternative transcripts):

NP_001238754.1 NP_003100.1 NP_612482.2

ENSEMBL proteins:

ENSP00000457804 ENSP00000329357 ENSP00000404263 ENSP00000458133

Reactome Protein details: P08047
Human Recombinant Protein Products for SP1:

	Browse Purified and Recombinant Proteins at EMD Millipore
	Browse R&D Systems for human recombinant proteins
	Enzo Life Sciences proteins for SP1
	Browse OriGene full length recombinant human proteins expressed in human HEK293 cells Browse OriGene Protein Over-expression Lysates OriGene Custom MassSpec OriGene Custom Protein Services for SP1
	GenScript Custom Purified and Recombinant Proteins Services for SP1
	Novus Biologicals SP1 Protein
	Browse Sino Biological Recombinant Proteins Browse Sino Biological Cell Lysates
	Browse ProSpec Recombinant Proteins
	Browse Proteins at Cloud-Clone Corp.

Gene Ontology (GO): 5 cellular component terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005634	nucleus	IDA	--
GO:0005654	nucleoplasm	TAS	--
GO:0005730	NOT nucleolus	IDA	--
GO:0005737	cytoplasm	IEA	--
GO:0032993	protein-DNA complex	IEA	--

SP1 for ontologies About GeneDecksing

SP1 Antibody Products:

	EMD Millipore Mono- and Polyclonal Antibodies for the study of SP1
	Browse R&D Systems for Antibodies
	Cell Signaling Technology (CST) Antibodies for SP1
	OriGene Antibodies for SP1 OriGene Custom Antibody Services for SP1
	GenScript Superior Antibodies for SP1
	Novus Biologicals SP1 Antibodies
	Abcam antibodies for SP1
	Browse Antibodies at Cloud-Clone Corp.
	ThermoFisher Antibody for SP1
	LSBio Antibodies in human, mouse, rat for SP1

Assay Products for SP1:

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Assay Services for SP1
	Browse R&D Systems for biochemical assays
	GenScript Custom Assay Services for SP1
	Browse Enzo Life Sciences for kits & assays
	Browse ELISAs at Cloud-Clone Corp. Browse CLIAs at Cloud-Clone Corp.

(According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

HGNC Gene Families:

SP: Specificity protein transcription factors

ZNF: Zinc fingers, C2H2-type

3 InterPro protein domains:

IPR015880 Znf_C2H2-like

IPR013087 Znf_C2H2/integrase_DNA-bd

IPR007087 Znf_C2H2

Graphical View of Domain Structure for InterPro Entry P08047

ProtoNet protein and cluster: P08047

UniProtKB/Swiss-Prot: SP1_HUMAN, P08047

Similarity: Belongs to the Sp1 C2H2-type zinc-finger protein family

Similarity: Contains 3 C2H2-type zinc fingers

SP1 for domains About GeneDecksing

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, Vector BioLabs, and Sirion Biotech, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene.)
About This Section

Molecular Function:

UniProtKB/Swiss-Prot Summary: SP1_HUMAN, P08047