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Aliases for SNAP25 Gene

Aliases for SNAP25 Gene

  • Synaptosome Associated Protein 25 2 3 5
  • Resistance To Inhibitors Of Cholinesterase 4 Homolog 2 3
  • Synaptosomal-Associated Protein, 25kDa 2 3
  • SNAP-25 3 4
  • SNAP 3 4
  • SUP 3 4
  • Synaptosomal-Associated 25 KDa Protein 4
  • Synaptosome Associated Protein 25kDa 3
  • Synaptosomal-Associated Protein 25 3
  • Super Protein 4
  • DJ1068F16.2 3
  • BA416N4.2 3
  • CMS18 3
  • RIC-4 3
  • RIC4 3
  • SEC9 3

External Ids for SNAP25 Gene

Previous HGNC Symbols for SNAP25 Gene

  • SNAP

Previous GeneCards Identifiers for SNAP25 Gene

  • GC20P010187
  • GC20P010194
  • GC20P010147
  • GC20P010199
  • GC20P010151

Summaries for SNAP25 Gene

Entrez Gene Summary for SNAP25 Gene

  • Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

GeneCards Summary for SNAP25 Gene

SNAP25 (Synaptosome Associated Protein 25) is a Protein Coding gene. Diseases associated with SNAP25 include Myasthenic Syndrome, Congenital, 18 and Presynaptic Congenital Myasthenic Syndromes. Among its related pathways are Metabolism and Transmission across Chemical Synapses. GO annotations related to this gene include calcium-dependent protein binding and SNAP receptor activity. An important paralog of this gene is SNAP23.

UniProtKB/Swiss-Prot for SNAP25 Gene

  • t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.

Gene Wiki entry for SNAP25 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for SNAP25 Gene

Genomics for SNAP25 Gene

Regulatory Elements for SNAP25 Gene

Enhancers for SNAP25 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH20F010215 1.4 FANTOM5 ENCODE 6.8 -0.9 -917 4.0 PKNOX1 MLX ZFP64 ARID4B SIN3A DMAP1 YY1 SLC30A9 ZNF143 DEK SNAP25 ENSG00000232448
GH20F010282 1 ENCODE 6 +64.2 64248 1.0 ATF1 MLX ARID4B DMAP1 ZNF48 YY1 SLC30A9 SP5 NFYC MXD4 SNAP25 GC20P010311 GC20M010220 GC20M010223
GH20F010555 1.3 Ensembl ENCODE 4.5 +339.1 339099 4.6 PKNOX1 ATF1 MLX ARID4B DMAP1 YBX1 SLC30A9 FOS SP5 NFYC JAG1 MKKS SNAP25 GC20P010506 LOC100421490
GH20F010185 0.7 ENCODE 8.2 -32.5 -32518 1.2 ELF3 HMG20B TEAD1 FOXA3 HMG20A HHEX RARA CEBPA SMAD4 NFE2 SNAP25 ENSG00000232448
GH20F010306 1 Ensembl ENCODE 5.4 +88.3 88299 2.3 JUN INSM2 SIN3A FEZF1 ZNF664 PRDM10 FOS TCF7L2 HLF BHLHE40 SNAP25 GC20P010311 GC20M010220 GC20M010223
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around SNAP25 on UCSC Golden Path with GeneCards custom track

Promoters for SNAP25 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00001447166 -328 3000 PKNOX1 MLX ZFP64 ARID4B SIN3A DMAP1 YY1 SLC30A9 ZNF143 DEK

Genomic Location for SNAP25 Gene

Chromosome:
20
Start:
10,218,829 bp from pter
End:
10,307,420 bp from pter
Size:
88,592 bases
Orientation:
Plus strand

Genomic View for SNAP25 Gene

Genes around SNAP25 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
SNAP25 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for SNAP25 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for SNAP25 Gene

Proteins for SNAP25 Gene

  • Protein details for SNAP25 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P60880-SNP25_HUMAN
    Recommended name:
    Synaptosomal-associated protein 25
    Protein Accession:
    P60880
    Secondary Accessions:
    • B2RAU4
    • D3DW16
    • D3DW17
    • P13795
    • P36974
    • P70557
    • P70558
    • Q53EM2
    • Q5U0B5
    • Q8IXK3
    • Q96FM2
    • Q9BR45

    Protein attributes for SNAP25 Gene

    Size:
    206 amino acids
    Molecular mass:
    23315 Da
    Quaternary structure:
    • Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A; this complex binds CPLX1 (PubMed:11832227). Found in a complex containing SYT1, SV2B and syntaxin-1 (By similarity). Found in a ternary complex with STX1A and VAMP8 (By similarity). Isoform 1 and isoform 2 interact with BLOC1S6 (PubMed:19546860). Interacts with CENPF (By similarity). Interacts with EQTN (By similarity). Interacts with HGS (By similarity). Interacts with KCNB1 (via N-terminus); reduces the voltage-dependent potassium channel KCNB1 activity in pancreatic beta cells (By similarity). Interacts with OTOF (By similarity). Interacts with RIMS1 (By similarity). Interacts with SNAPIN (By similarity). Interacts with STXBP6 (By similarity). Interacts with TRIM9 (By similarity). Interacts with ZDHHC13 (via ANK repeats) (By similarity). Interacts with ZDHHC17 (via ANK repeats) (By similarity). Associates with the BLOC-1 complex (PubMed:19546860).
    Miscellaneous:
    • When cloned and expressed in Eschericia coli, where protein palmitoylation does not occur, Cys-85, Cys-88, Cys-90 and Cys-92 in the protein sequence readily form an iron-sulfur cluster instead.

    Three dimensional structures from OCA and Proteopedia for SNAP25 Gene

    Alternative splice isoforms for SNAP25 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for SNAP25 Gene

Post-translational modifications for SNAP25 Gene

  • Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association (By similarity).
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

No data available for DME Specific Peptides for SNAP25 Gene

Domains & Families for SNAP25 Gene

Gene Families for SNAP25 Gene

Protein Domains for SNAP25 Gene

InterPro:
Blocks:
ProtoNet:

Suggested Antigen Peptide Sequences for SNAP25 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

P60880

UniProtKB/Swiss-Prot:

SNP25_HUMAN :
  • Contains 2 t-SNARE coiled-coil homology domains.
  • Belongs to the SNAP-25 family.
Domain:
  • Contains 2 t-SNARE coiled-coil homology domains.
Family:
  • Belongs to the SNAP-25 family.
genes like me logo Genes that share domains with SNAP25: view

Function for SNAP25 Gene

Molecular function for SNAP25 Gene

GENATLAS Biochemistry:
synaptosomal-associated protein 1,25kDa,soluble N ethylmaleimide-sensitive factor-attachment protein receptor,SNARE protein,palmitoylated in vivo for the binding to syntaxin
UniProtKB/Swiss-Prot Function:
t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.

Gene Ontology (GO) - Molecular Function for SNAP25 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005484 SNAP receptor activity IBA --
GO:0005515 protein binding IPI 8692999
GO:0017075 syntaxin-1 binding IPI 12730201
GO:0048306 calcium-dependent protein binding ISS --
genes like me logo Genes that share ontologies with SNAP25: view
genes like me logo Genes that share phenotypes with SNAP25: view

Human Phenotype Ontology for SNAP25 Gene

HPO Id HPO Name Alternative Ids Definition Synonyms

Animal Models for SNAP25 Gene

MGI Knock Outs for SNAP25:

Animal Model Products

Inhibitory RNA Products

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , Transcription Factor Targets and HOMER Transcription for SNAP25 Gene

Localization for SNAP25 Gene

Subcellular locations from UniProtKB/Swiss-Prot for SNAP25 Gene

Cytoplasm, perinuclear region. Cell membrane; Lipid-anchor. Cell junction, synapse, synaptosome. Note=Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region. Colocalizes with KCNB1 at the cell membrane (By similarity). {ECO:0000250, ECO:0000250 UniProtKB:P60881}.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for SNAP25 gene
Compartment Confidence
plasma membrane 5
cytosol 5
golgi apparatus 4
cytoskeleton 3
nucleus 3
extracellular 2
mitochondrion 1
endosome 1

Gene Ontology (GO) - Cellular Components for SNAP25 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm ISS --
GO:0005802 trans-Golgi network IEA --
GO:0005829 cytosol TAS --
GO:0005886 plasma membrane TAS --
GO:0008021 synaptic vesicle IEA --
genes like me logo Genes that share ontologies with SNAP25: view

Pathways & Interactions for SNAP25 Gene

genes like me logo Genes that share pathways with SNAP25: view

SIGNOR curated interactions for SNAP25 Gene

Is activated by:

Gene Ontology (GO) - Biological Process for SNAP25 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001504 neurotransmitter uptake NAS 8760387
GO:0007268 chemical synaptic transmission NAS 8760387
GO:0007269 neurotransmitter secretion TAS --
GO:0007626 locomotory behavior IEA --
GO:0008306 associative learning IEA --
genes like me logo Genes that share ontologies with SNAP25: view

Drugs & Compounds for SNAP25 Gene

(17) Drugs for SNAP25 Gene - From: DrugBank, DGIdb, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Botulinum Toxin Type A Approved, Investigational Pharma inhibitor, Target 0

(12) Additional Compounds for SNAP25 Gene - From: Novoseek and HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
Fe2+
  • Armco iron
  • Carbonyl iron
  • FE
  • Ferrovac e
  • Hematite
15438-31-0
genes like me logo Genes that share compounds with SNAP25: view

Transcripts for SNAP25 Gene

Unigene Clusters for SNAP25 Gene

Synaptosomal-associated protein, 25kDa:
Representative Sequences:

Inhibitory RNA Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for SNAP25 Gene

ExUns: 1a · 1b · 1c · 1d · 1e ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6 ^ 7a · 7b ^ 8 ^ 9a · 9b · 9c ^ 10a · 10b · 10c ^ 11a · 11b ^ 12a · 12b · 12c · 12d
SP1: - - - - - -
SP2: - - - - - - -
SP3: - - - - - - - -
SP4: - - - - - - -
SP5: - - - - -
SP6: - - - -
SP7: - - -
SP8: - - - - - - - - - - - - - - -
SP9: - - - - - - - - -
SP10: -
SP11:

Relevant External Links for SNAP25 Gene

GeneLoc Exon Structure for
SNAP25
ECgene alternative splicing isoforms for
SNAP25

Expression for SNAP25 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for SNAP25 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for SNAP25 Gene

This gene is overexpressed in Brain - Frontal Cortex (BA9) (x8.1), Brain - Cerebellar Hemisphere (x8.0), Brain - Anterior cingulate cortex (BA24) (x6.6), Brain - Cerebellum (x4.4), Brain - Amygdala (x4.3), Brain - Hippocampus (x4.3), and Brain - Cortex (x4.3).

Protein differential expression in normal tissues from HIPED for SNAP25 Gene

This gene is overexpressed in Brain (35.0) and Frontal cortex (27.7).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for SNAP25 Gene



Protein tissue co-expression partners for SNAP25 Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of SNAP25 Gene:

SNAP25

SOURCE GeneReport for Unigene cluster for SNAP25 Gene:

Hs.167317

mRNA Expression by UniProt/SwissProt for SNAP25 Gene:

P60880-SNP25_HUMAN
Tissue specificity: Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
genes like me logo Genes that share expression patterns with SNAP25: view

Orthologs for SNAP25 Gene

This gene was present in the common ancestor of animals.

Orthologs for SNAP25 Gene

Organism Taxonomy Gene Similarity Type Details
oppossum
(Monodelphis domestica)
Mammalia SNAP25 35
  • 100 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia SNAP25 35
  • 100 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia SNAP25 34 35
  • 99.68 (n)
dog
(Canis familiaris)
Mammalia SNAP25 34 35
  • 97.41 (n)
cow
(Bos Taurus)
Mammalia SNAP25 34 35
  • 96.28 (n)
mouse
(Mus musculus)
Mammalia Snap25 34 16 35
  • 95.15 (n)
rat
(Rattus norvegicus)
Mammalia Snap25 34
  • 94.66 (n)
chicken
(Gallus gallus)
Aves SNAP25 34 35
  • 90.78 (n)
lizard
(Anolis carolinensis)
Reptilia SNAP25 35
  • 97 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia snap25 34
  • 87.7 (n)
Str.15491 34
African clawed frog
(Xenopus laevis)
Amphibia snap25-prov 34
zebrafish
(Danio rerio)
Actinopterygii snap25b 35
  • 90 (a)
OneToMany
snap25a 34 35
  • 84.15 (n)
fruit fly
(Drosophila melanogaster)
Insecta Snap24 36 34 35
  • 64.2 (n)
Snap25 36 35
  • 61 (a)
worm
(Caenorhabditis elegans)
Secernentea Y22F5A.3 36
  • 53 (a)
ric-4 35
  • 46 (a)
ManyToMany
aex-4 35
  • 18 (a)
ManyToMany
sea squirt
(Ciona savignyi)
Ascidiacea CSA.6593 35
  • 65 (a)
OneToMany
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.8011 34
Species where no ortholog for SNAP25 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for SNAP25 Gene

ENSEMBL:
Gene Tree for SNAP25 (if available)
TreeFam:
Gene Tree for SNAP25 (if available)

Paralogs for SNAP25 Gene

Paralogs for SNAP25 Gene

(1) SIMAP similar genes for SNAP25 Gene using alignment to 1 proteins:

genes like me logo Genes that share paralogs with SNAP25: view

Variants for SNAP25 Gene

Sequence variations from dbSNP and Humsavar for SNAP25 Gene

SNP ID Clin Chr 20 pos Sequence Context AA Info Type
VAR_073698 Myasthenic syndrome, congenital, 18 (CMS18) [MIM:616330]
rs797044873 Likely pathogenic 10,284,751(+) CTTTG(G/T)TTATG reference, missense
rs10084501 -- 10,282,839(+) AGAAA(A/G)GAGCA intron-variant
rs1013044 -- 10,240,396(-) cctgc(A/G)tgtat intron-variant
rs1022511 -- 10,240,008(-) AGACT(C/T)GTGAT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for SNAP25 Gene

Variant ID Type Subtype PubMed ID
nsv952985 CNV deletion 24416366
nsv833919 CNV gain 17160897
nsv828687 CNV loss 20364138
nsv522874 CNV loss 19592680
nsv3290 CNV insertion 18451855
esv3645206 CNV loss 21293372
esv3645205 CNV loss 21293372
esv3556820 CNV deletion 23714750
esv2674016 CNV deletion 23128226

Variation tolerance for SNAP25 Gene

Residual Variation Intolerance Score: 43.5% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.22; 4.99% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for SNAP25 Gene

Human Gene Mutation Database (HGMD)
SNAP25
SNPedia medical, phenotypic, and genealogical associations of SNPs for
SNAP25

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for SNAP25 Gene

Disorders for SNAP25 Gene

MalaCards: The human disease database

(13) MalaCards diseases for SNAP25 Gene - From: OMIM, ClinVar, GeneTests, Orphanet, Swiss-Prot, DISEASES, Novoseek, and GeneCards

Disorder Aliases PubMed IDs
myasthenic syndrome, congenital, 18
  • cms18
presynaptic congenital myasthenic syndromes
  • congenital myasthenic syndromes, presynaptic
snap25-related congenital myasthenic syndrome
  • cms18
foodborne botulism
  • botulism
tetanus
  • clostridial tetanus
- elite association - COSMIC cancer census association via MalaCards

UniProtKB/Swiss-Prot

SNP25_HUMAN
  • Myasthenic syndrome, congenital, 18 (CMS18) [MIM:616330]: A form of congenital myasthenic syndrome, a group of disorders characterized by failure of neuromuscular transmission, including pre-synaptic, synaptic, and post-synaptic disorders that are not of autoimmune origin. Clinical features are easy fatigability and muscle weakness affecting the axial and limb muscles (with hypotonia in early-onset forms), the ocular muscles (leading to ptosis and ophthalmoplegia), and the facial and bulbar musculature (affecting sucking and swallowing, and leading to dysphonia). The symptoms fluctuate and worsen with physical effort. CMS18 is an autosomal dominant presynaptic disorder clinically characterized by early-onset muscle weakness and easy fatigability associated with delayed psychomotor development and ataxia. {ECO:0000269 PubMed:25381298}. Note=The disease is caused by mutations affecting the gene represented in this entry.

Relevant External Links for SNAP25

Genetic Association Database (GAD)
SNAP25
Human Genome Epidemiology (HuGE) Navigator
SNAP25
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
SNAP25
genes like me logo Genes that share disorders with SNAP25: view

No data available for Genatlas for SNAP25 Gene

Publications for SNAP25 Gene

  1. Evidence that putative ADHD low risk alleles at SNAP25 may increase the risk of schizophrenia. (PMID: 19132710) Carroll L.S. … Williams N.M. (Am. J. Med. Genet. B Neuropsychiatr. Genet. 2009) 3 22 46 64
  2. SNAP-25 genotype influences NAA/Cho in left hippocampus. (PMID: 18726138) Scherk H. … Gruber O. (J Neural Transm 2008) 3 22 46 64
  3. Associations of SNAP-25 polymorphisms with cognitive dysfunctions in Caucasian patients with schizophrenia during a brief trail of treatment with atypical antipsychotics. (PMID: 18347838) Spellmann I. … Riedel M. (Eur Arch Psychiatry Clin Neurosci 2008) 3 22 46 64
  4. No association between tagging SNPs of SNARE complex genes (STX1A, VAMP2 and SNAP25) and schizophrenia in a Japanese population. (PMID: 18512733) Kawashima K. … Iwata N. (Am. J. Med. Genet. B Neuropsychiatr. Genet. 2008) 3 22 46 64
  5. [Association between SNAP-25 gene polymorphism and attention deficit hyperactivity disorder]. (PMID: 19099826) Zhang H.Y. … Chen J.C. (Zhonghua Er Ke Za Zhi 2008) 3 22 46 64

Products for SNAP25 Gene

Sources for SNAP25 Gene

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