SMAD5 Gene

Aliases
for SMAD5 gene

Previous GC identifers: GC05P135545 GC05P130657

Summaries
for SMAD5 gene

Genomic Views
for SMAD5 gene

RefSeq DNA sequence:

NC_000005.9  NC_018916.1  NT_034772.6  

SwitchGear Promoter luciferase reporter plasmid: SMAD5 promoter sequence

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat SMAD5

SMAD5 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)


GeneLoc information about chromosome 5         GeneLoc Exon Structure

GeneLoc location for GC05P135496:  view genomic region     (about GC identifiers)

Proteins
for SMAD5 gene

Explore the universe of human proteins at neXtProt for SMAD5: NX_Q99717

Post-translational modifications:

	Browse Purified and Recombinant Proteins at EMD Millipore
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	Browse recombinant and purified proteins available from Enzo Life Sciences
	Browse OriGene full length recombinant human proteins expressed in human HEK293 cells OriGene Protein Over-expression Lysate (see all 4): SMAD5 OriGene Custom Protein Services for SMAD5
	GenScript Custom Purified and Recombinant Proteins Services for SMAD5
	Novus Biologicals SMAD5 Proteins Novus Biologicals SMAD5 Lysates
	Browse Sino Biological Recombinant Proteins
	Browse ProSpec Recombinant Proteins
	Uscn Proteins for SMAD5

Gene Ontology (GO): 5/7 cellular component terms (GO ID links to tree view) (see all 7):    About this table

	Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	R&D Systems Antibodies for SMAD5
	Cell Signaling Technology (CST) Antibodies for SMAD5
	OriGene Antibodies (see all 3): SMAD5 OriGene Custom Antibody Services for SMAD5
	GenScript Custom Superior Antibodies Services for SMAD5
	Novus Biologicals SMAD5 Antibodies
	Abcam antibodies for SMAD5
	Uscn Antibodies for SMAD5
	ThermoFisher Antibody for SMAD5

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Immunoassay Development Browse OriGene Fluorogenic Cell Assay Kits
	R&D Systems ELISAs for SMAD5
	GenScript Custom Assay Services for SMAD5
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for SMAD5

Protein Domains /
Families
for SMAD5 gene

ProtoNet protein and cluster: Q99717

Function
for SMAD5 gene

     Gene Ontology (GO): 5/7 molecular function terms (GO ID links to tree view) (see all 7):    About this table

(inferred from 4 alleles) (MGI details for Smad5)

inGenious Targeting Laboratory - Customizable classic, inducible, and humanized mouse model solutions for SMAD5

miRNA Products:		miRTarBase miRNAs that target SMAD5: hsa-mir-155 (MIRT000973)
		OriGene 3'-UTR Clone (see all 3): SMAD5 Browse MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat SMAD5
		8/99 QIAGEN miScript miRNA Assays for microRNAs that regulate SMAD5 (see all 99):
		hsa-miR-323-3p hsa-miR-607 hsa-miR-106a hsa-miR-15a hsa-miR-128 hsa-miR-374a hsa-miR-519a hsa-miR-93
		Browse SwitchGear 3'UTR luciferase reporter plasmids

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for SMAD5 (see all 7) OriGene shRNA RFP: SMAD5 OriGene siRNA: SMAD5
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat SMAD5
		Sirion Biotech Custom design and validation of potent shRNA sequences against SMAD5

Gene Editing Products:			DNA2.0 Custom Protein Engineering Service for SMAD5
			Sirion Biotech Customized adenovirus for overexpression of SMAD5  Sirion Biotech Customized adenovirus for potent knockdown of SMAD5

Clone Products:		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for SMAD5 (see all 7) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for SMAD5 (see all 4) OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector (see all 3): SMAD5 (NM_005903)
		Sino Biological Human cDNA Clone for SMAD5
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		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat SMAD5

Cell Line Products:		GenScript Custom overexpressing Cell Line Services for SMAD5
		Search LifeMap BioReagents cell lines for SMAD5
		Sirion Biotech Customized stable knockdown cell line services for SMAD5  Sirion Biotech Customized inducible knockdown cell line services for SMAD5 Sirion Biotech Customized stable overexpressing cell line services for SMAD5 Sirion Biotech Customized inducible overexpressing cell line services for SMAD5

In Situ Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for SMAD5

Pathways & Interactions
for SMAD5 gene

	Super-pathway	contained gene-specific pathways
1	Ubiquitin-dependent degradation of the Smad complex terminates BMP2 signalling	Ubiquitin-dependent degradation of the Smad complex terminates BMP2 signalling 1.00 ALK2 signaling events 0.31 Ubiquitin-dependent degradation controls basal levels of R-Smad1/5/8 0.38
2	Signaling by BMP	Signaling by BMP 1.00 BMP receptor signaling 0.31
3	TGF-beta/Smad Signaling	TGF-beta/Smad Signaling 1.00
4	Id Signaling Pathway	Id Signaling Pathway 1.00
5	TGF Beta Signaling Pathway	TGF Beta Signaling Pathway 1.00

2 R&D Systems Pathways for SMAD5

	Th17 Differentiation
	TGF-beta Signaling Pathways

1 Cell Signaling Technology (CST) Pathway for SMAD5

TGF-beta/Smad Signaling

5 BioSystems Pathways for SMAD5

	TGF Beta Signaling Pathway
	Id Signaling Pathway
	ALK2 signaling events
	ALK1 signaling events
	BMP receptor signaling

4        Reactome Pathways for SMAD5

	Ubiquitin-dependent degradation controls basal levels of R-Smad1/5/8
	Ubiquitin-dependent degradation of the Smad complex terminates BMP2 signalling
	Signal Transduction
	Signaling by BMP

1         Kegg Pathway  (Kegg details for SMAD5)

TGF-beta signaling pathway

Gene Ontology (GO): 5/21 biological process terms (GO ID links to tree view) (see all 21):    About this table

Drugs & Compounds
for SMAD5 gene

Transcripts
for SMAD5 gene

Expression
for SMAD5 gene

SMAD5 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: CTTCAATTCT

About this image

SMAD5 expression in embryonic tissues and stem cells
Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table

6 LifeMap In Vivo Development Anatomical Compartments/Cells
	Tissue Anatomical Compartment Cell			Category (developmental path)
	Spinal CordPreganglionic ColumnPreganglionic Motor Neurons			Motor Neurons
	Extraembryonic MesodermExtraembryonic MesodermExtraembryonic Mural Cells			Extraembryonic Tissues
	Lateral Plate MesodermSplanchnic MesodermMural Cells			Pericytes, Smooth Muscle
	BrainCerebral Cortex			Brain
	KidneyMetanephros			Kidney
	LiverLiver Bud			Liver
Expression: Positive    Negative     Selective marker Experimental details: Curated     Microarrays     In-situ hybridization

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for SMAD5 Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT2 qPCR Primer Assay in human, mouse, rat SMAD5
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat SMAD5
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat SMAD5

In Situ Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for SMAD5

Orthologs
for SMAD5 gene

Paralogs
for SMAD5 gene

Paralogs for SMAD5 gene

SMAD2²  SMAD3²  SMAD1²  SMAD6²  SMAD9²  SMAD4²  SMAD7²  

9 SIMAP similar genes for SMAD5 using alignment to 7 protein entries:     SMAD5_HUMAN (see all proteins):

DKFZp781O1323    MADH5    SMAD1    SMAD9    DKFZp586M0622    DKFZp686J10186

SMAD3    SMAD4    SMAD2

SMAD5 for paralogs           About GeneDecksing

Genomic Variants
for SMAD5 gene

10/819 NCBI SNPs in SMAD5 are shown (see all 819)     About this table

Genomic Data					Transcription Related Data				Allele Frequencies
SNP ID	Valid	Clinical significance	Chr 5 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
-1st2nd3rd	-1st2nd3rd	-1st2nd3rd	-1st2nd3rd	--	--	--	-1st2nd3rd	--	--	-1st2nd3rd	-1st2nd3rd	-1st2nd3rd
rs1138355081,2		--	130656021(+)	TTAGGG/ATGTCA	3	--	us2k1		1		CSA	2
rs748917341,2		--	130656439(+)	GGGCAC/GTCCCA	3	--	us2k1		0	--	--	--	--
rs745179041,2		--	130656613(+)	AGATTT/CGTATT	3	--	us2k1		2		CSA WA	120
rs791708831,2		--	130657563(+)	CGAAGC/TTGTCC	3	--	int1		2		CSA WA	120
rs1113267941,2	C	--	130658512(+)	GTGAAG/TTAGTT	3	--	int1		1		WA	2
rs757730801,2		--	130659567(+)	ATTATG/CATTTG	3	--	int1		1		WA	118
rs43806521,2	H	--	130659607(+)	GAACGG/TAGGCA	3	--	int1		4		NS EA	404
rs1136990031,2		--	130660689(+)	ACTAAG/ATGGGA	3	--	int1		1		CSA	2
rs779421701,2	F	--	130660910(+)	TTCAGT/CGACGC	3	--	int1		1		NA	120
rs2010500851,2	C	--	130661685(+)	GCTGGG/TGCTTT	3	--	int1		0	--	--	--	--

HapMap Linkage Disequilibrium report for SMAD5 (135468534 - 135524435 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 2 variations for SMAD5
     2 CNVs: 59235 3577

SABiosciences Cancer Mutation PCR Assays

QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing SMAD5

DNA2.0 Custom Variant and Variant Library Synthesis for SMAD5

Disorders / Diseases
for SMAD5 gene

17 diseases for SMAD5

4 Novoseek disease relationships for SMAD5 gene    About this table

Publications
for SMAD5 gene

PubMed articles for SMAD5 gene, integrated from 9 sources (see all 137): (articles sorted by number of sources associating them with SMAD5)

Utopia: connect your pdf to the dynamic world of online information

1. Mad-related genes in the human. (PubMed id 8673135)^{1, 2, 3} Riggins G.J....Vogelstein B. (1996)
2. Smad5 and DPC4 are key molecules in mediating BMP-2-induced osteoblastic differentiation of the pluripotent mesenchymal precursor cell line C2C12. (PubMed id 9442019)^{1, 2, 9} Nishimura R.... Yoneda T. (1998)
3. Smad5, a tumor suppressor candidate at 5q31.1, is hemizygously lost and not mutated in the retained allele in human leukemia cell line HL60. (PubMed id 9264367)^{1, 2, 9} Zavadil J.... Michalova K. (1997)
4. Suv39h histone methyltransferases interact with Smads and cooperate in BMP-induced repression. (PubMed id 15107829)^{1, 2, 9} Frontelo P....Lechleider R.J. (2004)
5. Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PubMed id 17081983)^{1, 2} Olsen J.V....Mann M. (2006)
6. The integral inner nuclear membrane protein MAN1 physically interacts with the R-Smad proteins to repress signaling by the transforming growth factor-{beta} superfamily of cytokines. (PubMed id 15647271)^{1, 2} Pan D.... Luo K. (2005)
7. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{1, 2} Gerhard D.S....Malek J. (2004)
8. TGF-beta signaling by Smad proteins. (PubMed id 10708949)^{1, 2} Miyazono K. (2000)
9. The Smad pathway. (PubMed id 10708948)^{1, 2} Wrana J.L. and Attisano L. (2000)
10. Remarkable versatility of Smad proteins in the nucleus of transforming growth factor-beta activated cells. (PubMed id 10647776)^{1, 2} Verschueren K. and Huylebroeck D. (1999)

External Searches for SMAD5 gene

Genome Databases showing SMAD5 gene

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Specialized Databases showing SMAD5 gene

Products
for SMAD5 gene