Set Analyses:
Advanced Search

Advanced Search

 
Search By
Section (entire)
for


 



SLC7A8 Gene

protein-coding   GIFtS: 59
GCID: GC14M023594

Solute Carrier Family 7 (Amino Acid Transporter Light Chain,...

  See related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

TryGeneCards Plus

Aliases
Solute Carrier Family 7 (Amino Acid Transporter Light Chain, L System),
Member 81 2
     Large Neutral Amino Acids Transporter Small Subunit 22
LAT22 3 5     Solute Carrier Family 7 (Amino Acid Transporter, L-Type), Member 82
L-Type Amino Acid Transporter 22 3     Solute Carrier Family 7 (Cationic Amino Acid Transporter, Y+ System),
Member 82
LPI-PC12     hLAT23
Integral Membrane Protein E16H2     Solute Carrier Family 7 Member 83

External Ids:    HGNC: 110661   Entrez Gene: 234282   Ensembl: ENSG000000920687   OMIM: 6042355   UniProtKB: Q9UHI53   

Export aliases for SLC7A8 gene to outside databases

Previous GC identifers: GC14M020966 GC14M017381 GC14M021584 GC14M022664 GC14M003711


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

TryGeneCards Plus

GeneCards Summary for SLC7A8 Gene:
SLC7A8 (solute carrier family 7 (amino acid transporter light chain, L system), member 8) is a protein-coding gene. Diseases associated with SLC7A8 include lysinuric protein intolerance, and cystinuria. GO annotations related to this gene include neutral amino acid transmembrane transporter activity and peptide antigen binding. An important paralog of this gene is SLC7A7.

UniProtKB/Swiss-Prot: LAT2_HUMAN, Q9UHI5
Function: Sodium-independent, high-affinity transport of small and large neutral amino acids such as alanine,
serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with
SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower
affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in
basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when
administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and
toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective
transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of
neutral amino acids from the epithelial cells to the bloodstream in the kidney

Gene Wiki entry for SLC7A8 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
About This Section

TryGeneCards Plus
RefSeq DNA sequence:
NC_000014.9  NC_018925.2  NT_026437.13  
Regulatory elements:
   Regulatory transcription factor binding sites in the SLC7A8 gene promoter:
         MZF-1   PPAR-gamma1   FAC1   AML1a   Ik-2   HSF2   Sox9   PPAR-gamma2   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 2): SLC7A8 promoter sequence
   Search Chromatin IP Primers for SLC7A8

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat SLC7A8


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 14q11.2   Ensembl cytogenetic band:  14q11.2   HGNC cytogenetic band: 14q11.2

SLC7A8 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
SLC7A8 gene location

GeneLoc information about chromosome 14         GeneLoc Exon Structure

GeneLoc location for GC14M023594:  view genomic region     (about GC identifiers)

Start:
23,594,504 bp from pter      End:
23,652,883 bp from pter
Size:
58,380 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
About This Section

TryGeneCards Plus

UniProtKB/Swiss-Prot: LAT2_HUMAN, Q9UHI5 (See protein sequence)
Recommended Name: Large neutral amino acids transporter small subunit 2  
Size: 535 amino acids; 58382 Da
Subunit: Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc
Miscellaneous: L-leucine transport activity inhibited by small zwitterionic amino acids (i.e. glycine, alanine,
serine, threonine asparginine, glutamine, methionine, leucine, isoleucine, valine, phenylalanine, tyrosine,
tryptophan, histidine and cysteine) and by glutamine and asparginine. Methionine uptake was inhibited by the
L-system substrates L-leucine, 2-amino-bicyclo-(2,2,1)-heptane-2-carboxylate (BCH), L-cysteine and by the
MeHg-L-cysteine complex and structurally related S-ethyl-L-cysteine. MeHg-L-cysteine uptake is inhibited by
L-methionine, L-leucine, BCH and S-ethyl-L-cysteine. L-leucine uptake was inhibited by L-CNSO
Sequence caution: Sequence=CAD62616.1; Type=Erroneous initiation; Note=Translation N-terminally shortened;
Secondary accessions: B2R8Q4 B4DKT4 B4DTV6 D3DS46 F2Z2J4 Q86U05 Q9UKQ6 Q9UKQ7 Q9UKQ8 Q9Y445
Alternative splicing: 4 isoforms:  Q9UHI5-1   Q9UHI5-2   Q9UHI5-3   Q9UHI5-4   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for SLC7A8: NX_Q9UHI5

Explore proteomics data for SLC7A8 at MOPED

Post-translational modifications: 

  • Modification sites at neXtProt
  • Modification sites at PhosphoSitePlus

  • See SLC7A8 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (4 alternative transcripts): 
    NP_001253965.1  NP_001253966.1  NP_036376.2  NP_877392.1  

    ENSEMBL proteins: 
     ENSP00000343949   ENSP00000320378   ENSP00000435114   ENSP00000391577   ENSP00000434345  
     ENSP00000435865   ENSP00000434352   ENSP00000436665   ENSP00000416398  
    Reactome Protein details: Q9UHI5

    SLC7A8 Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse OriGene full length recombinant human proteins expressed in human HEK293 cells
    OriGene Protein Over-expression Lysate for SLC7A8
    OriGene Custom MassSpec
    OriGene Custom Protein Services for SLC7A8
    GenScript Custom Purified and Recombinant Proteins Services for SLC7A8
    Novus Biologicals SLC7A8 Proteins
    Novus Biologicals SLC7A8 Lysates
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Browse Proteins at Cloud-Clone Corp.

    SLC7A8 Antibody Products:

    Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse R&D Systems for Antibodies
    OriGene Antibodies for SLC7A8
    OriGene Custom Antibody Services for SLC7A8
    Novus Biologicals SLC7A8 Antibodies
    Abcam antibodies for SLC7A8
    Browse Antibodies at Cloud-Clone Corp.
    Search ThermoFisher Antibodies for SLC7A8
    LSBio Antibodies in human, mouse, rat for SLC7A8

    SLC7A8 Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for SLC7A8
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for SLC7A8
    Browse Enzo Life Sciences for kits & assays
    Browse ELISAs at Cloud-Clone Corp.
    Browse CLIAs at Cloud-Clone Corp.


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    TryGeneCards Plus
    HGNC Gene Families:
    SLC: Solute carriers

    IUPHAR Guide to PHARMACOLOGY protein family classification: L-type amino acid transporter 2
    SLC7 family

    2 InterPro protein domains:
     IPR004760 L_AA_transporter
     IPR002293 AA/rel_permease1

    Graphical View of Domain Structure for InterPro Entry Q9UHI5

    ProtoNet protein and cluster: Q9UHI5

    UniProtKB/Swiss-Prot: LAT2_HUMAN, Q9UHI5
    Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter
    (LAT) (TC 2.A.3.8) family


    SLC7A8 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: LAT2_HUMAN, Q9UHI5
    Function: Sodium-independent, high-affinity transport of small and large neutral amino acids such as alanine,
    serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with
    SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower
    affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in
    basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when
    administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and
    toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective
    transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of
    neutral amino acids from the epithelial cells to the bloodstream in the kidney
    Biophysicochemical properties: Kinetic parameters: KM=221 uM for L-leucine; KM=64 uM for MeHg-L-cysteine; KM=161
    uM for methionine; KM=978 uM for L-alanine; KM=89.35 uM for L-phenylalanine; KM=57.3 uM for L-tryptophan; KM=48.8
    uM for L-tyrosine;
    Induction: Activity in polarized intestinal cells is regulated by the association between SLC3A2/4F2 (in the
    SLC3A2/4F2-LAT2 heterodimer) and ICAM1

         Genatlas biochemistry entry for SLC7A8:
    solute carrier family 7 member 8,cation aminoacid transport,highly expressed in skeletal
    muscle,intestine,kidney,placenta and in retinal pigmented epithelium,heterodimerizing with the cell surface
    glycoprotein 4F2hc/CD98 at the basolateral membrane to form a complex mediating y+L-type transporter exchanging
    cationic aminoacids and/or neutral aminoacids together with sodium,glycoprotein associated aminoacid transporter
    family,not mutated in lysinuric protein intolerance

         Gene Ontology (GO): Selected molecular function terms (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515protein binding IPI12716892
    GO:0015101organic cation transmembrane transporter activity IDA12117417
    GO:0015171amino acid transmembrane transporter activity ISS--
    GO:0015175neutral amino acid transmembrane transporter activity TAS10391915
    GO:0015179L-amino acid transmembrane transporter activity IEA--
         
    SLC7A8 for ontologies           About GeneDecksing


    Phenotypes:
         2 MGI mutant phenotypes (inferred from 1 allele(MGI details for Slc7a8):
     behavior/neurological  nervous system 

    SLC7A8 for phenotypes           About GeneDecksing

    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for SLC7A8
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for SLC7A8

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for SLC7A8
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for SLC7A8

    miRNA
    Products:
        
    miRTarBase miRNAs that target SLC7A8:
    hsa-mir-155-5p (MIRT005092)

    Block miRNA regulation of human, mouse, rat SLC7A8 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate SLC7A8 (see all 41):
    hsa-miR-548j hsa-miR-486-3p hsa-miR-766 hsa-miR-124 hsa-miR-9 hsa-miR-133a hsa-miR-3613-3p hsa-miR-548d-5p
    SwitchGear 3'UTR luciferase reporter plasmidSLC7A8 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for SLC7A8
    Predesigned siRNA for gene silencing in human, mouse, rat SLC7A8

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for SLC7A8

    Clone
    Products:
         
    OriGene clones in human, mouse for SLC7A8 (see all 12)
    OriGene ORF clones in mouse, rat for SLC7A8
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): SLC7A8 (NM_182728)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for SLC7A8
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat SLC7A8

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for SLC7A8
    Browse ESI BIO Cell Lines and PureStem Progenitors for SLC7A8 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for SLC7A8


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Subcellular locations from UniProtKB/Swiss-Prot
    LAT2_HUMAN, Q9UHI5: Cytoplasm. Basolateral cell membrane; Multi-pass membrane protein. Note=Localized to the
    cytoplasm when expressed alone but when coexpressed with SLC3A2/4F2hc, is localized to the plasma membrane.
    Colocalized with SLC3A2/4F2hc at the basolateral membrane of kidney cortex proximal tubules and small intestine
    epithelia of the villi
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    plasma membrane5
    endoplasmic reticulum1

    Gene Ontology (GO): Selected cellular component terms (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737cytoplasm IEA--
    GO:0005886plasma membrane TAS--
    GO:0005887integral component of plasma membrane TAS10391915
    GO:0016020membrane ----
    GO:0016021integral component of membrane ----

    SLC7A8 for ontologies           About GeneDecksing


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
    About This Section

    TryGeneCards Plus

    SuperPaths for SLC7A8 About    
    See pathways by source

    SuperPathContained pathways About
    1Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds
    SLC-mediated transmembrane transport0.47
    Transport of inorganic cations/anions and amino acids/oligopeptides0.38
    Transmembrane transport of small molecules0.47
    Amino acid transport across the plasma membrane0.32
    2Hemostasis
    Hemostasis0.43
    3Protein digestion and absorption
    Protein digestion and absorption
    4Cell surface interactions at the vascular wall
    Cell surface interactions at the vascular wall
    5Basigin interactions
    Basigin interactions

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    2 Reactome Pathways for SLC7A8
        Amino acid transport across the plasma membrane
    Basigin interactions


    1 Kegg Pathway  (Kegg details for SLC7A8):
        Protein digestion and absorption


    SLC7A8 for pathways           About GeneDecksing

        Pathway & Disease-focused RT2 Profiler PCR Array including SLC7A8: 
              Drug Transporters in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for SLC7A8

    STRING Interaction Network Preview (showing 4 interactants - click image to see more details)

    4 Interacting proteins for SLC7A8 (Q9UHI53 ENSP000003203784) via UniProtKB, MINT, STRING, and/or I2D
    InteractantInteraction Details
    GeneCardExternal ID(s)
    BAATQ140323, ENSP000002594074I2D: score=1 STRING: ENSP00000259407
    SLC3A1Q078373, ENSP000002606494I2D: score=1 STRING: ENSP00000260649
    SLC3A2P081953, ENSP000003671244I2D: score=2 STRING: ENSP00000367124
    UBCENSP000003448184STRING: ENSP00000344818
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 14):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003333amino acid transmembrane transport ----
    GO:0006520cellular amino acid metabolic process TAS10391915
    GO:0006810transport TAS10391915
    GO:0006811ion transport TAS--
    GO:0006865amino acid transport TAS--

    SLC7A8 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
    About This Section

    TryGeneCards Plus
    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for SLC7A8 (LAT2)

    6 HMDB Compounds for SLC7A8    About this table
    CompoundSynonyms CAS #PubMed Ids
    L-Alanine(2S)-2-Aminopropanoate (see all 35)56-41-7--
    L-Cysteine(+)-2-Amino-3-mercaptopropionic acid (see all 37)52-90-4--
    L-Glutamine(2S)-2,5-diamino-5-oxopentanoic acid (see all 31)56-85-9--
    L-Leucine(2S)-2-Amino-4-methylpentanoate (see all 16)61-90-5--
    L-Phenylalanine(-)-beta-Phenylalanine (see all 28)63-91-2--
    SodiumSodium (see all 2)7440-23-5--

    5 DrugBank Compounds for SLC7A8    About this table
    CompoundSynonyms CAS #TypeActionsPubMed Ids
    L-Glutamine(S)-2,5-diamino-5-oxopentanoic acid (see all 4)56-85-9transportersubstrate16785209 17273864 16308696 17504263 16249471
    L-Phenylalanine(S)-alpha-Amino-beta-phenylpropionic acid (see all 4)63-91-2target--12930836 10574970 12101005 9349567 12498902
    L-Alanine(2S)-2-Aminopropanoic acid (see all 15)56-41-7target--17504263
    Levodopa3,4-dihydroxyphenylalanine (see all 4)59-92-7transporter--15200428
    S-(Methylmercury)-L-Cysteine-- --transporter--12117417

    9 Novoseek inferred chemical compound relationships for SLC7A8 gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    2-aminobicyclo(2,2,1)heptane-2-carboxylic acid 93.4 1 10391915 (1)
    leucine 65.1 4 19890975 (2), 15863273 (1)
    l-amino acid 63.9 5 10673541 (1), 10391915 (1), 11230513 (1)
    melphalan 56.7 8 17558306 (4), 19075510 (1), 19447222 (1)
    cystine 52.1 4 12660317 (3)
    phenylalanine 34.9 3 17558306 (1), 10574970 (1)
    alanine 26.5 2 12660317 (1), 10574970 (1)
    cysteine 16.5 3 12660317 (2)
    threonine 15.4 1 12660317 (1)



    SLC7A8 for compounds           About GeneDecksing



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
    About This Section

    TryGeneCards Plus

    REFSEQ mRNAs for SLC7A8 gene (4 alternative transcripts): 
    NM_001267036.1  NM_001267037.1  NM_012244.3  NM_182728.2  

    Unigene Cluster for SLC7A8:

    Solute carrier family 7 (amino acid transporter light chain, L system), member 8
    Hs.596643  [show with all ESTs]
    Unigene Representative Sequence: NM_012244
    13 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000339733(uc001wiy.3) ENST00000316902(uc001wix.3 uc010tnk.2 uc010tnl.2 uc001wiz.3 uc010akj.3)
    ENST00000397310(uc001wiw.3) ENST00000469263 ENST00000453702 ENST00000529705
    ENST00000528860 ENST00000528806 ENST00000528186 ENST00000532568 ENST00000524758
    ENST00000525062 ENST00000422941
    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat SLC7A8 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate SLC7A8 (see all 41):
    hsa-miR-548j hsa-miR-486-3p hsa-miR-766 hsa-miR-124 hsa-miR-9 hsa-miR-133a hsa-miR-3613-3p hsa-miR-548d-5p
    SwitchGear 3'UTR luciferase reporter plasmidSLC7A8 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for SLC7A8
    Predesigned siRNA for gene silencing in human, mouse, rat SLC7A8
    Clone
    Products:
         
    OriGene clones in human, mouse for SLC7A8 (see all 12)
    OriGene ORF clones in mouse, rat for SLC7A8
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): SLC7A8 (NM_182728)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for SLC7A8
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat SLC7A8
    Primer
    Products:
        
    OriGene qPCR primer pairs and template standards for SLC7A8
    OriGene qSTAR qPCR primer pairs in human, mouse for SLC7A8
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat SLC7A8
      QuantiTect SYBR Green Assays in human, mouse, rat SLC7A8
      QuantiFast Probe-based Assays in human, mouse, rat SLC7A8

    Additional mRNA sequence: 

    AB037669.1 AF087908.1 AF087911.1 AF135828.1 AF135829.1 AF135830.1 AF171669.1 AK223610.1 
    AK296702.1 AK300384.1 AK307609.1 AK313465.1 AL365342.1 AL365343.2 AL365344.1 AL365345.1 
    AL365346.1 AL365347.1 AL365414.1 BC036825.1 BC052250.1 BX248288.1 NR_049767.1 Y18483.1 

    23 DOTS entries:

    DT.311443  DT.100697969  DT.92441563  DT.100791464  DT.92441552  DT.101982645  DT.100791459  DT.100791465 
    DT.95184464  DT.75200329  DT.101982647  DT.100791463  DT.120748368  DT.120748434  DT.95204226  DT.95257171 
    DT.100791466  DT.120748335  DT.120748412  DT.91748546  DT.91906748  DT.92441555  DT.120748487 

    Selected AceView cDNA sequences (see all 264):

    BM129717 CA417408 NM_012244 NM_182728 AI492024 BM129428 Z38621 BU194551 
    AA812880 BM906729 CD671134 R81562 AI249760 BU735576 BU846479 CK819644 
    BG403071 AI383143 AW273801 BM893825 CK819645 CB178024 CB068651 BF058899 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for SLC7A8 (see all 9)    About this scheme

    ExUns: 1a · 1b · 1c ^ 2a · 2b · 2c ^ 3a · 3b · 3c ^ 4 ^ 5a · 5b ^ 6 ^ 7 ^ 8 ^ 9 ^ 10a · 10b ^ 11 ^ 12a · 12b · 12c ^ 13a · 13b ^ 14a · 14b
    SP1:                                                              -     -     -                       -                 -           -     -                     
    SP2:                                                                          -                 -     -     -           -           -                           
    SP3:                                                              -     -     -                 -     -     -     -     -     -     -     -     -               
    SP4:                                                                          -                                                                                 
    SP5:                                                                                                                                                            


    ECgene alternative splicing isoforms for SLC7A8

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    TryGeneCards Plus

    SLC7A8 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: CTGTCTTGGG
    SLC7A8 Expression
    About this image


    SLC7A8 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 7) fully expand
     
     Endothelium (Cardiovascular System)    fully expand to see all 2 entries
             Endothelial Cells Blood Brain Barrier
     
     Brain (Nervous System)    fully expand to see all 2 entries
             Endothelial Cells Blood Brain Barrier
     
     Kidney (Urinary System)
             Metanephros
     
     NULL (Uncategorized)
             Mesenchymal Progenitor Cells - Passage 2
     
     Neural Ectoderm (Nervous System)
             Neural ectoderm cells
    SLC7A8 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    SLC7A8 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.596643

    UniProtKB/Swiss-Prot: LAT2_HUMAN, Q9UHI5
    Tissue specificity: Strongest expression is observed in kidney and moderate expression in placenta and brain,
    followed by liver, prostate, testis, ovary, lymph node, thymus, spleen, skeletal muscle and heart. Also expressed
    in fetal liver as well as in the retinal pigment epithelial cell line ARPE-19 and the intestinal epithelial cell
    line Caco-2

        Pathway & Disease-focused RT2 Profiler PCR Array including SLC7A8: 
              Drug Transporters in human mouse rat

    Primer
    Products:
    OriGene qPCR primer pairs and template standards for SLC7A8
    OriGene qSTAR qPCR primer pairs in human, mouse for SLC7A8
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat SLC7A8
    QuantiTect SYBR Green Assays in human, mouse, rat SLC7A8
    QuantiFast Probe-based Assays in human, mouse, rat SLC7A8
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for SLC7A8

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    TryGeneCards Plus

    This gene was present in the common ancestor of animals.

    Orthologs for SLC7A8 gene from Selected species (see all 16)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Slc7a81 , 5 solute carrier family 7 (cationic amino acid transporter, more1, 5 89.92(n)1
    93.38(a)1
      14 (27.91 cM)5
    509341  NM_016972.21  NP_058668.11 
     547222165 
    lizard
    (Anolis carolinensis)
    Reptilia SLC7A86
    solute carrier family 7 (amino acid transporter li...
    83(a)
    1 ↔ 1
    GL343776.1(37418-55327)
    African clawed frog
    (Xenopus laevis)
    Amphibia BC044971.12   -- 80.52(n)    BC044971.1 
    zebrafish
    (Danio rerio)
    Actinopterygii slc7a8a1 solute carrier family 7 (cationic amino acid transporter, more 75.59(n)
    84.77(a)
      100007704  NM_001271897.1  NP_001258826.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta mnd3
    gb1
    amino acid transporter3
    genderblind1
    50(a)
    (best of 3)3
    57.1(n)1
    46.62(a)1
      432651  NM_143279.21  NP_651536.11 
    worm
    (Caenorhabditis elegans)
    Secernentea F27C8.13 integral membrane protein E16 like 47(a)
    (best of 8)
      IV(9599614-9601874)   --


    ENSEMBL Gene Tree for SLC7A8 (if available)
    TreeFam Gene Tree for SLC7A8 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section

    TryGeneCards Plus
    Paralogs for SLC7A8 gene
    SLC7A72  SLC7A62  SLC7A112  SLC7A92  SLC7A52  SLC7A132  SLC7A102  
    14 SIMAP similar genes for SLC7A8 using alignment to 6 protein entries:     LAT2_HUMAN (see all proteins):
    SLC7A10    SLC7A5    SLC7A6    lat1    SLC7A5P1    DKFZp686K14185
    SLC7A5P2    SLC7A9    SLC7A7    xCT    hxCT    SLC7A11
    SLC7A1    SLC7A13

    SLC7A8 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
    About This Section

    TryGeneCards Plus

    Selected SNPs for SLC7A8 (see all 1299)    About this table    
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 14 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1422966171,2
    C--3771835(+) AAGATC/TGCACC 1 -- us2k10--------
    rs736004361,2
    C--3771975(+) TCTCTG/TTAGGT 1 -- us2k14Minor allele frequency- T:0.14WA CSA NA 241
    rs171274931,2
    C,F,H--3772076(+) ATATGT/CTGACA 1 -- us2k112Minor allele frequency- C:0.06NA NS EA WA CSA 923
    rs1497328411,2
    --3772198(+) GCTCTC/TGGCTC 1 -- us2k10--------
    rs726843541,2
    C--3772231(+) TTCAAG/ATGATT 1 -- us2k11Minor allele frequency- A:0.50NA 2
    rs104381261,2
    C--23594011(+) tttatC/Tttttg 5 -- int14Minor allele frequency- T:0.12NA WA EA 360
    rs1441505931,2
    C--23594050(+) GGCTGA/GTCTCA 5 -- int10--------
    rs1153935441,2
    C,F--23594110(+) GATTAC/TGGGAG 5 -- int11Minor allele frequency- T:0.03WA 118
    rs1115473421,2
    C,F--23594111(+) ATTACG/AGGAGT 5 -- int11Minor allele frequency- A:0.50NA 2
    rs1838024721,2
    --23594124(+) GCCGCC/TGTGCC 5 -- int10--------

    HapMap Linkage Disequilibrium report for SLC7A8 (23594504 - 23652883 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 2 variations for SLC7A8:    About this table    
    Variant IDTypeSubtypePubMed ID
    nsv1215CNV Insertion18451855
    nsv901492CNV Loss21882294

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing SLC7A8
    DNA2.0 Custom Variant and Variant Library Synthesis for SLC7A8

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    TryGeneCards Plus
    OMIM gene information: 604235    OMIM disorders: --

    14 diseases for SLC7A8:    
    About MalaCards
    lysinuric protein intolerance    cystinuria    leiomyoma    oral cancer
    endotheliitis    retinitis    cerebritis    sarcoma
    hypertension    thyroiditis    breast cancer    malaria
    prostatitis    neuronitis


    SLC7A8 for disorders           About GeneDecksing

    Genetic Association Database (GAD): SLC7A8
    Human Genome Epidemiology (HuGE) Navigator: SLC7A8 (7 documents)

    Export disorders for SLC7A8 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    TryGeneCards Plus

    PubMed articles for SLC7A8 gene, integrated from 10 sources (see all 62):
    (articles sorted by number of sources associating them with SLC7A8)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Identification of a membrane protein, LAT-2, that co-expresses with 4F2 heavy chain, an L-type amino acid transport activity with broad specificity for small and large zwitterionic amino acids. (PubMed id 10391915)1, 2, 3, 9 Pineda M.... Palacin M. (J. Biol. Chem. 1999)
    2. LAT2, a new basolateral 4F2hc/CD98-associated amino acid transporter of kidney and intestine. (PubMed id 10574970)1, 2, 7, 9 Rossier G....Kuehn L.C. (J. Biol. Chem. 1999)
    3. Transport of a neurotoxicant by molecular mimicry: the methylmercury- L-cysteine complex is a substrate for human L-type large neutral amino acid transporter (LAT) 1 and LAT2. (PubMed id 12117417)1, 2, 7 Simmons-Willis T.A.... Ballatori N. (Biochem. J. 2002)
    4. SLC7A7, encoding a putative permease-related protein, is mutated in patients with lysinuric protein intolerance. (PubMed id 10080183)1, 2, 3 Borsani G....Sebastio G. (Nat. Genet. 1999)
    5. Genetic polymorphisms in the amino acid transporters LAT1 and LAT2 in relation to the pharmacokinetics and side effects of melphalan. (PubMed id 17558306)1, 4, 9 KA1hne A....BrockmAPller J. (Pharmacogenet. Genomics 2007)
    6. Reabsorption of neutral amino acids mediated by amino acid transporter LAT2 and TAT1 in the basolateral membrane of proximal tubule. (PubMed id 15918515)1, 2, 9 Park S.Y.... Kim D.K. (Arch. Pharm. Res. 2005)
    7. Identification of stereoselective transporters for S-nitroso-L- cysteine: role of LAT1 and LAT2 in biological activity of S- nitrosothiols. (PubMed id 15769744)1, 2, 9 Li S. and Whorton A.R. (J. Biol. Chem. 2005)
    8. Association of 4F2hc with light chains LAT1, LAT2 or y+LAT2 requires different domains. (PubMed id 11311135)1, 2, 9 Broeer A.... Broeer S. (Biochem. J. 2001)
    9. CD98 and intracellular adhesion molecule I regulate the activity of amino acid transporter LAT-2 in polarized intestinal epithelia. (PubMed id 12716892)1, 2, 9 Liu X.... Merlin D. (J. Biol. Chem. 2003)
    10. Assessment of a polymorphism of SDK1 with hypertension in Japanese Individuals. (PubMed id 19851296)1, 4 Oguri M....Yamada Y. (Am. J. Hypertens. 2010)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

    TryGeneCards Plus
     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section

    TryGeneCards Plus
    Entrez Gene: 23428 HGNC: 11066 AceView: SLC7A8 Ensembl:ENSG00000092068 euGenes: HUgn23428
    ECgene: SLC7A8 Kegg: 23428 H-InvDB: SLC7A8

    (According to HUGE)
    About This Section

    TryGeneCards Plus
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

    TryGeneCards Plus
    NameDescription
    PharmGKB entry for SLC7A8 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

    TryGeneCards Plus
    Patent Information for SLC7A8 gene:
    Search GeneIP for patents involving SLC7A8

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, and ESI BIO, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
    About This Section

    TryGeneCards Plus

     
     EMD Millipore genomic analysis products

      
     Browse Antibodies   Browse Cell Culture Products  
     Browse ELISAs   Browse Flow Cytometry Kits  
     Browse Primer Pairs   Browse Enzyme Activity Assays/Reagents  
     Browse ELISpot/FluoroSpot Kits/Development Modules   Browse TFB/Immunoprecipitation Assays  
     Browse Apoptosis Detection Kits/Reagents   Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents  
     Browse DNA Damage/Repair Kits/Reagents   Browse Luminex Assays  
     Browse Cell Selection/Detection Kits/Reagents   Browse Secondary Antibodies/Controls/Staining Reagents  
     Browse Recombinant/Natural Proteins   Browse Stem Cell Products  
     Browse cDNA Clones   Browse Proteome Profiler Antibody Arrays  
     OriGene Antibodies for SLC7A8   OriGene RNAi products in human, mouse, rat for SLC7A8  
     OriGene qPCR primer pairs and template standards for SLC7A8   OriGene Protein Over-expression Lysate for SLC7A8  
     OriGene Custom Mass Spec   OriGene clones in human, mouse for SLC7A8  
     OriGene qSTAR qPCR primer pairs in human, mouse for SLC7A8   Browse OriGene full length recombinant human proteins expressed in human HEK293 cells  
     OriGene ORF clones in mouse, rat for SLC7A8   OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling  
     OriGene Custom Antibody Services for SLC7A8   OriGene Custom Protein Services for SLC7A8  

     
     
     Block miRNA regulation of human, mouse, rat SLC7A8 using miScript Target Protectors SeqTarget long-range PCR primers for resequencing SLC7A8
     DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat SLC7A8 Predesigned siRNA for gene silencing in human, mouse, rat SLC7A8
     QuantiFast Probe-based Assays in human, mouse, rat SLC7A8 QuantiTect SYBR Green Assays in human, mouse, rat SLC7A8
     PCR Arrays including human, mouse, rat SLC7A8 Search Chromatin IP Primers for SLC7A8
     Pre-validated RT2 qPCR Primer Assay in human, mouse, rat SLC7A8  GeneGlobe Interaction Network for SLC7A8
     Regulatory tfbs in SLC7A8 promoter
     GenScript Custom Purified and Recombinant Proteins Services for SLC7A8 GenScript cDNA clones with any tag delivered in your preferred vector for SLC7A8
     GenScript Custom Assay Services for SLC7A8 GenScript Custom overexpressing Cell Line Services for SLC7A8
     CloneReady with Over 120,000 Genes  Gene Synthesis: Any Gene in Any Vector
     Vector-based siRNA and miRNA, Ready for Transfection Gene Mutant Library, Variants up to 10^11
     Plasmid Preparation Custom Peptide Services
     Search for Antibodies & Assays

     Search Tocris compounds for SLC7A8 (LAT2)
     Browse Sino Biological Proteins
     Browse Sino Biological Cell Lysates
     Browse Sino Biological cDNA Clones
     4000+ Proteins
     Search Sino Biological for antibodies, proteins & pathways
     Protein Production Services
     Transfection Reagents
     Protein A/G/L resins
     Isotyping reagents
     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

     Novus Tissue Slides
     SLC7A8 antibodies
     SLC7A8 proteins
     SLC7A8 lysates
     Antibodies for SLC7A8
     See all of Abcam's Antibodies, Kits and Proteins for SLC7A8
     Custom Antibody / Protein Production Service
     Bulk Purchasing
     Advantages of Rabbit Monoclonal antibodies
     Abcam protocols and scientific support
     Browse ProSpec Recombinant Proteins
     Browse Proteins at Cloud-Clone Corp.
     Browse Antibodies at Cloud-Clone Corp.
     Browse ELISAs at Cloud-Clone Corp.
     Browse CLIAs at Cloud-Clone Corp.



     Browse ESI BIO Cell Lines and PureStem Progenitors for SLC7A8
     Gene Synthesis
     Protein Engineering
     Variant Library Synthesis
     Codon Optimization
     Protein Production and Purification
     Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for SLC7A8
     SwitchGear 3'UTR luciferase reporter plasmids for SLC7A8
     SwitchGear Promoter luciferase reporter plasmids for SLC7A8
     Search ThermoFisher Antibodies for SLC7A8
     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat SLC7A8
     inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for SLC7A8
     inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for SLC7A8
     LSBio Antibodies in human, mouse, rat for SLC7A8
    Customized transgenic rodents for:
     Humanization
     Biomarker expression
     Off-target effect monitoring
     Translational medicine
     Tissue-specific gene expresssion
     Time-controlled gene expresssion
     Browse compounds at ApexBio
           
    GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014

    View Random Gene

    Category
    (GIFtS: )
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 


    Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

    Hot genes      Disease genes      SLC7A8 gene at Home site.
    Version: 3.12.142 28 July 2014
    hostname: 356980-web2.xennexinc.com index build: 126 solr: 1.4