Set Analyses:
Advanced Search

Advanced Search

 
Search By
Section (entire)
for


 



SIRT5 Gene

protein-coding   GIFtS: 64
GCID: GC06P013574

Sirtuin 5

(Previous names: sirtuin (silent mating type information regulation 2, S.cerevisiae,...)
  Try our

disease database 

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

TryGeneCards Plus

Aliases
Sirtuin 51 2     NAD-Dependent Lysine Demalonylase And Desuccinylase Sirtuin-5,
Mitochondrial2
Regulatory Protein SIR2 Homolog 52 3     NAD-Dependent Protein Deacylase Sirtuin-5, Mitochondrial2
SIR2L52 3     Silent Mating Type Information Regulation 2, S.Cerevisiae, Homolog 52
SIR2-Like Protein 52 3     Sir2-Like 52
Sirtuin (Silent Mating Type Information Regulation 2 Homolog) 5 (S.
Cerevisiae)1
     Sirtuin Type 52
Sirtuin (Silent Mating Type Information Regulation 2, S.Cerevisiae,
Homolog) 51
     EC 3.5.1.-3
NAD-Dependent Deacetylase Sirtuin-52     

External Ids:    HGNC: 149331   Entrez Gene: 234082   Ensembl: ENSG000001245237   OMIM: 6044835   UniProtKB: Q9NXA83   

Export aliases for SIRT5 gene to outside databases

Previous GC identifers: GC06P013632 GC06P013682 GC06P013519


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

TryGeneCards Plus

Entrez Gene summary for SIRT5 Gene:
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the
sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human
sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene
silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as
intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is
included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript
variants. (provided by RefSeq, Jul 2010)

GeneCards Summary for SIRT5 Gene:
SIRT5 (sirtuin 5) is a protein-coding gene. GO annotations related to this gene include protein-malonyllysine demalonylase activity and NAD+ ADP-ribosyltransferase activity. An important paralog of this gene is SIRT1.

UniProtKB/Swiss-Prot: SIR5_HUMAN, Q9NXA8
Function: NAD-dependent lysine demalonylase and desuccinylase that specifically removes malonyl and succinyl
groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during
prolonged fasting: acts by mediating desuccinylation of CPS1, thereby increasing CPS1 activity in response to
elevated NAD levels during fasting. Activates SOD1 by mediating its desuccinylation, leading to reduced reactive
oxygen species. Has weak NAD-dependent protein deacetylase activity; however this activity may not be
physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro

summary for SIRT5 Gene:
Silent information regulator (Sir2)-like family deacetylases (also known as sirtuins) are a group of enzymes
closely related to histone deacetylases. These enzymes can be found in the cytoplasm, mitochondria or
nucleus and are ubiquitously expressed. Sir2-like family deacetylases catalyze the removal of acetyl groups
from lysine residues in histones and non-histone proteins, which is coupled to NAD+ hydrolysis. In general,
sirtuins do not act autonomously but as components of large multiprotein complexes, such as pRb-E2F and
mSin3A, that mediate important transcription regulatory pathways. Sirtuins have a role in regulation of
transcription and apoptosis leading to substantial interest in inhibitors of these enzymes as possible
antineoplastic agents. In addition, Sir2-like family deacteylases are involved in the normal ageing process
through their role in resistance to cellular stress.

Gene Wiki entry for SIRT5 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
About This Section

TryGeneCards Plus
RefSeq DNA sequence at NCBI GenBank:
NC_000006.11  NT_007592.16  NC_018917.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the SIRT5 gene promoter:
         POU2F1   HOXA3   Pbx1a   POU2F1a   AP-4   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidSIRT5 promoter sequence
   Search Chromatin IP Primers for SIRT5

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat SIRT5


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 6p23   Ensembl cytogenetic band:  6p23   HGNC cytogenetic band: 6p23

SIRT5 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
SIRT5 gene location

GeneLoc information about chromosome 6         GeneLoc Exon Structure

GeneLoc location for GC06P013574:  view genomic region     (about GC identifiers)

Start:
13,574,761 bp from pter      End:
13,615,390 bp from pter
Size:
40,630 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
About This Section

TryGeneCards Plus

UniProtKB/Swiss-Prot: SIR5_HUMAN, Q9NXA8 (See protein sequence)
Recommended Name: NAD-dependent protein deacylase sirtuin-5, mitochondrial precursor  
Size: 310 amino acids; 33881 Da
Cofactor: Binds 1 zinc ion per subunit
Subunit: Interacts with CPS1 (By similarity). Monomer. Homodimer. Forms homodimers upon suramin binding
Miscellaneous: The mechanism of demalonylation and desuccinylation involves the presence of a 1',2'-cyclic
intermediate, suggesting that sirtuins use the ADP-ribose-peptidylamidate mechanism to remove acyl groups from
substrate lysine residues (PubMed:22767592)
Selected PDB 3D structures from and Proteopedia for SIRT5 (see all 8):
2B4Y (3D)        2NYR (3D)        3RIG (3D)        3RIY (3D)        4F4U (3D)        4F56 (3D)    
Secondary accessions: B4DFM4 B4DYJ5 F5H5Z9 Q5T294 Q5T295 Q9Y6E6
Alternative splicing: 4 isoforms:  Q9NXA8-1   Q9NXA8-2   Q9NXA8-3   Q9NXA8-4   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for SIRT5: NX_Q9NXA8

Explore proteomics data for SIRT5 at MOPED

Post-translational modifications: 

  • Modification sites at neXtProt
  • Modification sites at PhosphoSitePlus

  • See SIRT5 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (4 alternative transcripts): 
    NP_001180196.1  NP_001229756.1  NP_036373.1  NP_112534.1  

    ENSEMBL proteins: 
     ENSP00000368552   ENSP00000352830   ENSP00000476211   ENSP00000368564   ENSP00000476228  
     ENSP00000380509  

    SIRT5 Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
    Browse R&D Systems for human recombinant proteins
    Enzo Life Sciences proteins for SIRT5
    OriGene Purified Protein for SIRT5
    OriGene Protein Over-expression Lysate for SIRT5
    OriGene MassSpec for SIRT5
    OriGene Custom Protein Services for SIRT5
    GenScript Custom Purified and Recombinant Proteins Services for SIRT5
    Novus Biologicals SIRT5 Proteins
    Novus Biologicals SIRT5 Lysate
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for SIRT5

     
    Search eBioscience for Proteins for SIRT5 

    SIRT5 Antibody Products:

    EMD Millipore Mono- and Polyclonal Antibodies for the study of SIRT5
    R&D Systems Antibodies for SIRT5 (Sirtuin 5/SIRT5)
    Cell Signaling Technology (CST) Antibodies for SIRT5 
    OriGene Antibodies for SIRT5
    OriGene Custom Antibody Services for SIRT5
    Novus Biologicals SIRT5 Antibodies
    Abcam antibodies for SIRT5
    Cloud-Clone Corp. Antibodies for SIRT5
    ThermoFisher Antibodies for SIRT5
    LSBio Antibodies in human, mouse, rat for SIRT5

    SIRT5 Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for SIRT5
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for SIRT5
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for SIRT5
    Cloud-Clone Corp. CLIAs for SIRT5
    Search eBioscience for ELISAs for SIRT5 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    TryGeneCards Plus
    4 InterPro protein domains:
     IPR027546 Sirtuin_class_III
     IPR026591 Sirtuin_cat_small_dom
     IPR003000 Sirtuin
     IPR026590 Ssirtuin_cat_dom

    Graphical View of Domain Structure for InterPro Entry Q9NXA8

    ProtoNet protein and cluster: Q9NXA8

    UniProtKB/Swiss-Prot: SIR5_HUMAN, Q9NXA8
    Domain: In contrast to class I sirtuins, class III sirtuins have only weak deacetylase activity. Difference in
    substrate specificity is probably due to a larger hydrophobic pocket with 2 residues (Tyr-102 and Arg-105) that
    bind to malonylated and succinylated substrates and define the specificity (PubMed:22076378)
    Similarity: Belongs to the sirtuin family. Class III subfamily
    Similarity: Contains 1 deacetylase sirtuin-type domain


    Find genes that share domains with SIRT5           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: SIR5_HUMAN, Q9NXA8
    Function: NAD-dependent lysine demalonylase and desuccinylase that specifically removes malonyl and succinyl
    groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during
    prolonged fasting: acts by mediating desuccinylation of CPS1, thereby increasing CPS1 activity in response to
    elevated NAD levels during fasting. Activates SOD1 by mediating its desuccinylation, leading to reduced reactive
    oxygen species. Has weak NAD-dependent protein deacetylase activity; however this activity may not be
    physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro
    Catalytic activity: NAD(+) + a malonylprotein = nicotinamide + O-malonyl-ADP-ribose + a protein
    Catalytic activity: NAD(+) + a succinylprotein = nicotinamide + O-succinyl-ADP-ribose + a protein
    Enzyme regulation: Inhibited by suramin. NAD-dependent lysine desuccinylase activity is inhibited by physiological
    nicotinamide concentrations, while deacetylase activity is not. In contrast, resveratrol activates deacetylase
    activity, while inhibiting desuccinylase activity
    Biophysicochemical properties: Kinetic parameters: KM=6.1 uM for a synthetic histone H3K9 malonyllysine peptide;
    KM=5.8 uM for a synthetic histone H3K9 succinyllysine peptide; KM=8.7 uM for a synthetic GLUD1 peptide
    malonylated at 'Lys- 503'; KM=14 uM for a synthetic GLUD1 peptide succinylated at 'Lys- 503'; KM=150 uM for a
    synthetic ACSS1 peptide malonylated at 'Lys- 628'; KM=450 uM for a synthetic ACSS1 peptide succinylated at 'Lys-
    628';

         Enzyme Number (IUBMB): EC 3.5.1.-1

         Gene Ontology (GO): Selected molecular function terms (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003950NOT NAD+ ADP-ribosyltransferase activity TAS17456799
    GO:0005515protein binding ----
    GO:0008270zinc ion binding IDA--
    GO:0036054protein-malonyllysine demalonylase activity IDA--
    GO:0036055protein-succinyllysine desuccinylase activity IDA--
         
    Find genes that share ontologies with SIRT5           About GenesLikeMe


    Phenotypes:
         2 GenomeRNAi human phenotypes for SIRT5:
     Increased cell death HMECs cel  Increased cell death in breast 

         2 MGI mutant phenotypes (inferred from 2 alleles(MGI details for Sirt5):
     homeostasis/metabolism  normal 

    Find genes that share phenotypes with SIRT5           About GenesLikeMe

    Animal Models:
         MGI mouse knock-outs for SIRT5: Sirt5tm1Fwa Sirt5tm1.1Ics

       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for SIRT5
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for SIRT5

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for SIRT5
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for SIRT5

    miRNA
    Products:
        
    miRTarBase miRNAs that target SIRT5:
    hsa-mir-335-5p (MIRT018521)

    Block miRNA regulation of human, mouse, rat SIRT5 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate SIRT5 (see all 17):
    hsa-miR-34b* hsa-miR-3607-3p hsa-miR-3133 hsa-miR-192 hsa-miR-186 hsa-miR-215 hsa-miR-548c-3p hsa-miR-576-5p
    SwitchGear 3'UTR luciferase reporter plasmidSIRT5 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for SIRT5
    Predesigned siRNA for gene silencing in human, mouse, rat SIRT5

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for SIRT5

    Clone
    Products:
         
    OriGene clones in human, mouse for SIRT5 (see all 16)
    OriGene ORF clones in mouse, rat for SIRT5
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 3): SIRT5 (NM_012241)
    Sino Biological Human cDNA Clone for SIRT5
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for SIRT5
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat SIRT5
    Addgene plasmids for SIRT5 

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for SIRT5
    Browse ESI BIO Cell Lines and PureStem Progenitors for SIRT5 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for SIRT5


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Subcellular locations from UniProtKB/Swiss-Prot
    SIR5_HUMAN, Q9NXA8: Mitochondrion matrix. Mitochondrion intermembrane space. Cytoplasm, cytosol. Nucleus.
    Note=Mainly mitochondrial. Also present extramitochondrially: a fraction is present in the cytosol and very small
    amounts are also detected in the nucleus
    SIR5_HUMAN, Q9NXA8: Isoform 1: Cytoplasm. Mitochondrion
    SIR5_HUMAN, Q9NXA8: Isoform 2: Mitochondrion
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    mitochondrion5
    cytosol2
    nucleus2

    Gene Ontology (GO): 5 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus TAS--
    GO:0005739mitochondrion IDA--
    GO:0005758mitochondrial intermembrane space IDA18680753
    GO:0005759mitochondrial matrix IDA18680753
    GO:0005829cytosol IDA--

    Find genes that share ontologies with SIRT5           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
    About This Section

    TryGeneCards Plus

    SuperPaths for SIRT5 About   (see all 7)  
    See pathways by source

    SuperPathContained pathways About
    1p53 Signaling
    p53 Signaling0.38
    p53 Pathway0.38
    2Nicotinate and nicotinamide metabolism
    NAD metabolism0.43
    3Non-homologous end-joining
    DNA damage NHEJ mechanisms of DSBs repair0.37
    44-1BB Pathway
    SUMO Pathway0.30
    5Signaling events mediated by HDAC Class I
    Signaling events mediated by HDAC Class I


    Find genes that share SuperPaths with SIRT5           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    2 Downloadable PowerPoint Slides of GeneGlobe Pathway Central Maps for SIRT5
        p53 Signaling
    SUMO Pathway

    2 Cell Signaling Technology (CST) Pathways for SIRT5
        Chromatin Regulation / Acetylation
    Glucose / Energy Metabolism

    2 GeneGo (Thomson Reuters) Pathways for SIRT5
        NAD metabolism
    DNA damage NHEJ mechanisms of DSBs repair

    1 BioSystems Pathway for SIRT5
        Signaling events mediated by HDAC Class I

    1 Sino Biological Pathway for SIRT5
        p53 Pathway


        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for SIRT5
    Interactions:

        GeneGlobe Interaction Network for SIRT5

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for SIRT5 (Q9NXA83 ENSP000003685524) via UniProtKB, MINT, STRING, and/or I2D (see all 182)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    SIRT3Q9NTG73, ENSP000003721914I2D: score=1 STRING: ENSP00000372191
    RELAQ042063, ENSP000003842734I2D: score=1 STRING: ENSP00000384273
    CPS1P313273, ENSP000004026084I2D: score=1 STRING: ENSP00000402608
    CHEK2ENSP000003720234STRING: ENSP00000372023
    BRD4ENSP000002633774STRING: ENSP00000263377
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006342chromatin silencing TAS10381378
    GO:0006471NOT protein ADP-ribosylation TAS17456799
    GO:0006476NOT protein deacetylation IDA--
    GO:0036047peptidyl-lysine demalonylation IDA--
    GO:0036048protein desuccinylation IDA--

    Find genes that share ontologies with SIRT5           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
    About This Section

    TryGeneCards Plus
    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
    6 ApexBio Compounds for SIRT5     About this table
    CompoundAction CAS #
    EX 527 (SEN0014196)novel, potential, and specific small-molecule inhibitor of SIRT1[49843-98-3]
    Sirtinolspecific SIRT1 and SIRT2 inhibitor[410536-97-9]
    sirtuin modulatorsirtuin modulator[1093403-33-8]
    SRT1720activating the sirtuin subtype SIRT1 in vitro. [1001645-58-4]
    Tenovin-3Tenovin-3 [1011301-27-1]
    Tenovin-6a potent SIRT1 and SIRT2 inhibitor as well as p53 activator. [1011557-82-6]

    Compounds for SIRT5 available from Tocris Bioscience    About this table
    CompoundAction CAS #
    SirtinolSelective sirtuin family deacetylase inhibitor[410536-97-9]
    EX 527Selective SIRT1 inhibitor[49843-98-3]
    AK 7Selective SIRT2 inhibitor; brain penetrant[420831-40-9]
    SplitomicinSir2p inhibitor[5690-03-9]
    AGK 2Selective SIRT2 inhibitor[304896-28-4]

    1 HMDB Compound for SIRT5    About this table
    CompoundSynonyms CAS #PubMed Ids
    NAD3-Carbamoyl-1-D-ribofuranosylpyridinium hydroxide 5'-ester with adenosine 5'-pyrophosphate (see all 28)53-84-9--

    5 DrugBank Compounds for SIRT5    About this table
    CompoundSynonyms CAS #TypeActionsPubMed Ids
    2'-O-Acetyl Adenosine-5-Diphosphoribose-- --target--17139284 17016423 10592235
    Adenosine-5-Diphosphoribose-- --target--17139284 17016423 10592235
    Nicotinamide-- 98-92-0target--17139284 17016423 10592235
    Nicotinamide-Adenine-Dinucleotide-- 53-84-9target--17139284 17016423 10592235
    SuraminBayer 205 (see all 6)145-63-1targetinhibitor17355872



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
    About This Section

    TryGeneCards Plus

    REFSEQ mRNAs for SIRT5 gene (4 alternative transcripts): 
    NM_001193267.2  NM_001242827.1  NM_012241.4  NM_031244.3  

    Unigene Clusters for SIRT5:

    Sirtuin 5
    Hs.567431  [show with all ESTs], Hs.594133
    Unigene Representative Sequences: NM_012241, AK094269
    6 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000379250(uc003nax.3 uc003nay.3) ENST00000359782(uc011dit.2)
    ENST00000606427 ENST00000379262(uc003naw.3) ENST00000606117 ENST00000397350

    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat SIRT5 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate SIRT5 (see all 17):
    hsa-miR-34b* hsa-miR-3607-3p hsa-miR-3133 hsa-miR-192 hsa-miR-186 hsa-miR-215 hsa-miR-548c-3p hsa-miR-576-5p
    SwitchGear 3'UTR luciferase reporter plasmidSIRT5 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for SIRT5
    Predesigned siRNA for gene silencing in human, mouse, rat SIRT5
    Clone
    Products:
         
    OriGene clones in human, mouse for SIRT5 (see all 16)
    OriGene ORF clones in mouse, rat for SIRT5
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 3): SIRT5 (NM_012241)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for SIRT5
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat SIRT5
    Addgene plasmids for SIRT5 
    Primer
    Products:
        
    OriGene qPCR primer pairs and template standards for SIRT5
    OriGene qSTAR qPCR primer pairs in human, mouse for SIRT5
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat SIRT5
      QuantiTect SYBR Green Assays in human, mouse, rat SIRT5
      QuantiFast Probe-based Assays in human, mouse, rat SIRT5

    Additional mRNA sequence: 

    AK094269.1 BC064478.1 

    16 DOTS entries:

    DT.212593  DT.409466  DT.99974806  DT.95143890  DT.101955149  DT.121393611  DT.95262507  DT.121393774 
    DT.99995211  DT.100791969  DT.100767818  DT.121393691  DT.95113447  DT.95212233  DT.97766929  DT.91744595 

    Selected AceView cDNA sequences (see all 127):

    BF434883 AA732428 BE350936 CR619396 BE217845 CD368433 AL704940 AA811503 
    CF140176 CR623350 AA765055 BC000126 NM_012241 CA412315 BX353902 CR600455 
    AI419476 CR595911 BQ006251 W68575 BI061941 AI269430 AA721145 CA406695 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for SIRT5 (see all 8)    About this scheme

    ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8a · 8b ^ 9 ^ 10 ^ 11a · 11b ^ 12 ^ 13 ^ 14a · 14b · 14c ^ 15a · 15b
    SP1:                    -                                               -                       -     -     -     -               
    SP2:              -     -                 -                             -                       -     -     -     -               
    SP3:                                                                    -                       -     -                           
    SP4:                                                                                                                              
    SP5:              -     -                                                                                                         


    ECgene alternative splicing isoforms for SIRT5

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    TryGeneCards Plus

    SIRT5 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: AAAATGAAAC
    SIRT5 Expression
    About this image


    SIRT5 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 3) fully expand
     
     Trophoblast (Extraembryonic Tissues)
             Trophoblast Cells Trophoblast
     
     Testis (Reproductive System)
             Leydig Cells Testis Interstitium
     
     Liver (Hepatobiliary System)
             Hepatocytes Liver Lobule
    SIRT5 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    SIRT5 Protein Expression

    SOURCE GeneReport for Unigene clusters: Hs.567431 Hs.594133

    UniProtKB/Swiss-Prot: SIR5_HUMAN, Q9NXA8
    Tissue specificity: Widely expressed

        Custom PCR Arrays for SIRT5
    Primer
    Products:
    OriGene qPCR primer pairs and template standards for SIRT5
    OriGene qSTAR qPCR primer pairs in human, mouse for SIRT5
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat SIRT5
    QuantiTect SYBR Green Assays in human, mouse, rat SIRT5
    QuantiFast Probe-based Assays in human, mouse, rat SIRT5
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for SIRT5

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    TryGeneCards Plus

    This gene was present in the common ancestor of animals and fungi.

    Orthologs for SIRT5 gene from Selected species (see all 17)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Sirt51 , 5 sirtuin 5 (silent mating type information regulation more5
    sirtuin 51
    84.09(n)1
    86.13(a)1
      13 (21.60 cM)5
    683461  NM_178848.31  NP_849179.11 
     433707105 
    chicken
    (Gallus gallus)
    Aves SIRT51 sirtuin 5 73.66(n)
    77.7(a)
      420834  NM_001276364.1  NP_001263293.1 
    lizard
    (Anolis carolinensis)
    Reptilia SIRT56
    sirtuin 5
    76(a)
    1 ↔ 1
    4(57071843-57082950)
    African clawed frog
    (Xenopus laevis)
    Amphibia Xl.154942 Xenopus laevis transcribed sequence with weak similarity more 75.03(n)    CA973198.1 
    zebrafish
    (Danio rerio)
    Actinopterygii CK353135.12   -- 74.94(n)    CK353135.1 
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes HST16
    SIR26
    NAD(+)-dependent histone deacetylase; essential su...
    Conserved NAD+ dependent histone deacetylase of th...
    13(a)
    11(a)
    many ↔ many
    many ↔ many
    XV(200368-201879) YOL068C
    IV(376757-378445) YDL042C


    ENSEMBL Gene Tree for SIRT5 (if available)
    TreeFam Gene Tree for SIRT5 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section

    TryGeneCards Plus
    Paralogs for SIRT5 gene
    SIRT12  SIRT42  
    1 SIMAP similar gene for SIRT5 using alignment to 2 protein entries:     SIR5_HUMAN (see all proteins):
    DKFZp686L2375

    Find genes that share paralogs with SIRT5           About GenesLikeMe


    3 Pseudogenes.org Pseudogenes for SIRT5
    PGOHUM00000244750 PGOHUM00000260404 PGOHUM00000249355


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
    About This Section

    TryGeneCards Plus

    Selected SNPs for SIRT5 (see all 993)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 6 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs773418131,2
    F--13572782(+) CTACAT/CATCGG 3 -- us2k11Minor allele frequency- C:0.03WA 118
    rs770680381,2
    F--13572842(+) TCCAAC/TTGAAA 3 -- us2k10--------
    rs1833239701,2
    --13573085(+) GAGAAG/TGAGTA 4 -- us2k10--------
    rs1890740311,2
    --13573115(+) CCCTTC/TTTCCA 4 -- us2k10--------
    rs38070531,2
    C,F,H--13573138(+) GCTGGG/CGAATT 4 -- us2k112Minor allele frequency- C:0.16EA NS NA WA 2270
    rs1918265561,2
    --13573299(+) ATAATA/GATGTA 4 -- us2k10--------
    rs1840179561,2
    --13573439(+) GTTACC/TTATAG 4 -- us2k10--------
    rs38405441,2
    C--13573459(+) CTAGG-/AAAAAA 4 -- us2k11Minor allele frequency- A:0.50CSA 2
    rs2005801511,2
    --13573467(+) AAAAA-/TCAAAC 4 -- us2k10--------
    rs1869721071,2
    --13573505(+) CATTTC/TCAGCT 4 -- us2k10--------

    HapMap Linkage Disequilibrium report for SIRT5 (13574761 - 13615390 bp)

    Structural Variations
          Database of Genomic Variants (DGV) variations for SIRT5: --
    Human Gene Mutation Database (HGMD): SIRT5
    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing SIRT5
    DNA2.0 Custom Variant and Variant Library Synthesis for SIRT5

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    TryGeneCards Plus
    OMIM gene information: 604483    OMIM disorders: --


    Find genes that share disorders with SIRT5           About GenesLikeMe

    Genetic Association Database (GAD): SIRT5
    Human Genome Epidemiology (HuGE) Navigator: SIRT5 (4 documents)

    Export disorders for SIRT5 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    TryGeneCards Plus

    PubMed articles for SIRT5 gene, integrated from 10 sources (see all 43):
    (articles sorted by number of sources associating them with SIRT5)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Characterization of five human cDNAs with homology to the yeast SIR2 gene: Sir2-like proteins (sirtuins) metabolize NAD and may have protein ADP-ribosyltransferase activity. (PubMed id 10381378)1, 2, 3 Frye R.A. (Biochem. Biophys. Res. Commun. 1999)
    2. Substrates and regulation mechanisms for the human mitochondrial sirtuins Sirt3 and Sirt5. (PubMed id 18680753)1, 2, 9 Schlicker C....Steegborn C. (J. Mol. Biol. 2008)
    3. SIRT5 desuccinylates and activates SOD1 to eliminate ROS. (PubMed id 24140062)1, 2 Lin Z.F.... Chen X. (Biochem. Biophys. Res. Commun. 2013)
    4. The bicyclic intermediate structure provides insights into the desuccinylation mechanism of human sirtuin 5 (SIRT5). (PubMed id 22767592)1, 2 Zhou Y....Hao Q. (J. Biol. Chem. 2012)
    5. Sirt5 deacylation activities show differential sensitivities to nicotinamide inhibition. (PubMed id 23028781)1, 2 Fischer F....Steegborn C. (PLoS ONE 2012)
    6. The first identification of lysine malonylation substrates and its regulatory enzyme. (PubMed id 21908771)1, 2 Peng C.... Zhao Y. (Mol. Cell. Proteomics 2011)
    7. Distinct regulation of mitochondrial localization and stability of two human Sirt5 isoforms. (PubMed id 21143562)1, 2 Matsushita N.... Yanagi S. (Genes Cells 2011)
    8. Sirt5 is a NAD-dependent protein lysine demalonylase and desuccinylase. (PubMed id 22076378)1, 2 Du J.... Lin H. (Science 2011)
    9. Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression. (PubMed id 20877624)1, 4 Hendrickson S.L....O'Brien S.J. (PLoS ONE 2010)
    10. Investigation of genetic susceptibility factors for human longevity - a targeted nonsynonymous SNP study. (PubMed id 20800603)1, 4 Flachsbart F....Nebel A. (Mutat. Res. 2010)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

    TryGeneCards Plus
     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section

    TryGeneCards Plus
    Entrez Gene: 23408 HGNC: 14933 AceView: SIRT5 Ensembl:ENSG00000124523 euGenes: HUgn23408
    ECgene: SIRT5 H-InvDB: SIRT5

    (According to HUGE)
    About This Section

    TryGeneCards Plus
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

    TryGeneCards Plus
    NameDescription
    PharmGKB entry for SIRT5 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

    TryGeneCards Plus
    Patent Information for SIRT5 gene:
    Search GeneIP for patents involving SIRT5

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
    About This Section

    TryGeneCards Plus

     Browse Kits and Assays available from EMD Millipore
     Browse Purified and Recombinant Proteins at EMD Millipore
     Browse Small Molecules at EMD Millipore
     EMD Millipore Mono- and Polyclonal Antibodies for the study of SIRT5
     
     EMD Millipore genomic analysis products

      
     Antibodies for SIRT5 (Sirtuin 5/SIRT5)   Browse Cell Culture Products  
     Browse ELISAs   Browse Flow Cytometry Kits  
     Browse Primer Pairs   Browse Enzyme Activity Assays/Reagents  
     Browse ELISpot/FluoroSpot Kits/Development Modules   Browse TFB/Immunoprecipitation Assays  
     Browse Apoptosis Detection Kits/Reagents   Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents  
     Browse DNA Damage/Repair Kits/Reagents   Browse Luminex Assays  
     Browse Cell Selection/Detection Kits/Reagents   Browse Secondary Antibodies/Controls/Staining Reagents  
     Browse Recombinant/Natural Proteins   Browse Stem Cell Products  
     Browse cDNA Clones   Browse Proteome Profiler Antibody Arrays  
     OriGene Antibodies for SIRT5   OriGene RNAi products in human, mouse, rat for SIRT5  
     OriGene qPCR primer pairs and template standards for SIRT5   OriGene Protein Over-expression Lysate for SIRT5  
     OriGene MassSpec something-or-other for SIRT5   OriGene clones in human, mouse for SIRT5  
     OriGene qSTAR qPCR primer pairs in human, mouse for SIRT5   OriGene Purified Protein for SIRT5  
     OriGene ORF clones in mouse, rat for SIRT5   OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling  
     OriGene Custom Antibody Services for SIRT5   OriGene Custom Protein Services for SIRT5  

     
     
     Block miRNA regulation of human, mouse, rat SIRT5 using miScript Target Protectors SeqTarget long-range PCR primers for resequencing SIRT5
     DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat SIRT5 Predesigned siRNA for gene silencing in human, mouse, rat SIRT5
     QuantiFast Probe-based Assays in human, mouse, rat SIRT5 QuantiTect SYBR Green Assays in human, mouse, rat SIRT5
     Custom PCR Arrays for SIRT5 Search Chromatin IP Primers for SIRT5
     Pre-validated RT2 qPCR Primer Assay in human, mouse, rat SIRT5  GeneGlobe Interaction Network for SIRT5
     Regulatory tfbs in SIRT5 promoter
     GenScript Custom Purified and Recombinant Proteins Services for SIRT5 GenScript cDNA clones with any tag delivered in your preferred vector for SIRT5
     GenScript Custom Assay Services for SIRT5 GenScript Custom overexpressing Cell Line Services for SIRT5
     CloneReady with Over 120,000 Genes  Gene Synthesis: Any Gene in Any Vector
     Vector-based siRNA and miRNA, Ready for Transfection Gene Mutant Library, Variants up to 10^11
     Plasmid Preparation Custom Peptide Services
     Antibodies & Assays for SIRT5 

     Tocris compounds for SIRT5
     Browse Sino Biological Proteins
     Browse Sino Biological Cell Lysates
     cDNA Clones for SIRT5
     4000+ Proteins
     Search Sino Biological for antibodies, proteins & pathways
     Protein Production Services
     Transfection Reagents
     Protein A/G/L resins
     Isotyping reagents
     Proteins for SIRT5
     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

     Novus Tissue Slides
     SIRT5 antibodies
     SIRT5 proteins
     SIRT5 lysates
     Antibodies for SIRT5
     See all of Abcam's Antibodies, Kits and Proteins for SIRT5
     Custom Antibody / Protein Production Service
     Bulk Purchasing
     Advantages of Rabbit Monoclonal antibodies
     Abcam protocols and scientific support
     Browse ProSpec Recombinant Proteins
     Proteins for SIRT5
     Antibodies for SIRT5
     ELISAs for SIRT5
     CLIAs for SIRT5



     Browse ESI BIO Cell Lines and PureStem Progenitors for SIRT5
     Gene Synthesis
     Protein Engineering
     Variant Library Synthesis
     Codon Optimization
     Protein Production and Purification
     Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for SIRT5
     SwitchGear 3'UTR luciferase reporter plasmids for SIRT5
     SwitchGear Promoter luciferase reporter plasmids for SIRT5
     ThermoFisher Antibodies for SIRT5
     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat SIRT5
     inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for SIRT5
     inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for SIRT5
     LSBio Antibodies in human, mouse, rat for SIRT5
    Customized transgenic rodents for:
     Humanization
     Biomarker expression
     Off-target effect monitoring
     Translational medicine
     Tissue-specific gene expresssion
     Time-controlled gene expresssion
     ApexBio compounds for SIRT5
     Addgene plasmids for SIRT5
      Search eBioscience for proteins for SIRT5
      Search eBioscience for elisas for SIRT5
      eBioscience FlowRNA Probe Sets
           
    GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014 , 21 Aug 2014 , 8 Sep 2014

    View Random Gene

    Category
    (GIFtS: )
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 


    Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

    Hot genes      Disease genes      SIRT5 gene at Home site.
    Version: 3.12.224 13 Oct 2014
    hostname: 356977-web1.xennexinc.com index build: 128 solr: 1.4