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SIAH3 Gene

protein-coding   GIFtS: 40
GCID: GC13M046353

Siah E3 Ubiquitin Protein Ligase Family Member 3

(Previous name: seven in absentia homolog 3 (Drosophila))
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(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Siah E3 Ubiquitin Protein Ligase Family Member 31 2
Seven In Absentia Homolog 3 (Drosophila)1
Seven In Absentia Homolog 32
siah-32
Siah-33

External Ids:    HGNC: 305531   Entrez Gene: 2835142   Ensembl: ENSG000002154757   OMIM: 6156095   UniProtKB: Q8IW033   

Export aliases for SIAH3 gene to outside databases

Previous GC identifers: GC13M045252 GC13M027151


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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GeneCards Summary for SIAH3 Gene:
SIAH3 (siah E3 ubiquitin protein ligase family member 3) is a protein-coding gene. An important paralog of this gene is SIAH2.


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000013.10  NC_018924.2  NT_024524.15  
Regulatory elements:
   Regulatory transcription factor binding sites in the SIAH3 gene promoter:
         E2F-3a   E2F-4   E2F-5   GATA-3   POU3F2 (N-Oct-5a)   E2F-2   POU3F2   GATA-2   E2F-1   E2F   
         Other transcription factors

Browse SwitchGear Promoter luciferase reporter plasmids
   Search Chromatin IP Primers for SIAH3

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat SIAH3


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 13q14.13   Ensembl cytogenetic band:  13q14.13   HGNC cytogenetic band: 13q14.12

SIAH3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
SIAH3 gene location

GeneLoc information about chromosome 13         GeneLoc Exon Structure

GeneLoc location for GC13M046353:  view genomic region     (about GC identifiers)

Start:
46,354,405 bp from pter      End:
46,425,871 bp from pter
Size:
71,467 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., eBioscience, and/or antibodies-online,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., eBioscience, and/or antibodies-online, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, Cloud-Clone Corp, antibodies-online, and/or others.)
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UniProtKB/Swiss-Prot: SIAH3_HUMAN, Q8IW03 (See protein sequence)
Recommended Name: Seven in absentia homolog 3  
Size: 269 amino acids; 30660 Da
Sequence caution: Sequence=AAH41372.2; Type=Erroneous initiation; Sequence=BAC04809.1; Type=Miscellaneous
discrepancy; Note=Chimeric cDNA. A chimeric cDNA originating from chromosomes 13 and 19;
Secondary accessions: B7ZBP0 Q8N8M6

Explore the universe of human proteins at neXtProt for SIAH3: NX_Q8IW03

Explore proteomics data for SIAH3 at MOPED


See SIAH3 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

REFSEQ proteins: NP_942146.2  
ENSEMBL proteins: 
 ENSP00000383256  

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(According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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3 InterPro protein domains:
 IPR008974 TRAF-like
 IPR018121 7-in-absentia-prot_TRAF-dom
 IPR004162 SINA_like

Graphical View of Domain Structure for InterPro Entry Q8IW03

ProtoNet protein and cluster: Q8IW03

UniProtKB/Swiss-Prot: SIAH3_HUMAN, Q8IW03
Similarity: Belongs to the SINA (Seven in absentia) family
Similarity: Contains 1 SIAH-type zinc finger


Find genes that share domains with SIAH3           About GenesLikeMe


(According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, genOway,
transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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     Gene Ontology (GO): 2 molecular function terms:    About this table

GO IDQualified GO termEvidencePubMed IDs
GO:0005515protein binding ----
GO:0046872metal ion binding IEA--
     
Find genes that share ontologies with SIAH3           About GenesLikeMe


Animal Models:
   genOway: Develop your customized and physiologically relevant rodent model for SIAH3

miRNA
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Selected qRT-PCR Assays for microRNAs that regulate SIAH3 (see all 26):
hsa-miR-146b-3p hsa-miR-548k hsa-miR-29a hsa-miR-29c hsa-miR-767-5p hsa-miR-1265 hsa-miR-3613-3p hsa-miR-24
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(According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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Subcellular locations from COMPARTMENTS: 

CompartmentConfidence
mitochondrion2
nucleus2
cytosol1
extracellular1

Gene Ontology (GO): 1 cellular component term:    About this table

GO IDQualified GO termEvidencePubMed IDs
GO:0005634nucleus IEA--

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(SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for SIAH3
Interactions:

    Search GeneGlobe Interaction Network for SIAH3

STRING Interaction Network Preview (showing 5 interactants - click image to see more details)

5 Interacting proteins for SIAH3 (ENSP000003832564) via UniProtKB, MINT, STRING, and/or I2D
InteractantInteraction Details
GeneCardExternal ID(s)
SPRY1ENSP000003437854STRING: ENSP00000343785
NCOR1ENSP000002687124STRING: ENSP00000268712
NCOR2ENSP000003485514STRING: ENSP00000348551
--ENSP000003488164STRING: ENSP00000348816
--ENSP000003231724STRING: ENSP00000323172
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Gene Ontology (GO): 2 biological process terms:    About this table

GO IDQualified GO termEvidencePubMed IDs
GO:0006511ubiquitin-dependent protein catabolic process IEA--
GO:0007275multicellular organismal development IEA--

Find genes that share ontologies with SIAH3           About GenesLikeMe



(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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Browse Small Molecules at EMD Millipore
   Browse drugs & compounds from Enzo Life Sciences
  Browse compounds at ApexBio 

Browse Tocris compounds for SIAH3



(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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REFSEQ mRNAs for SIAH3 gene: 
NM_198849.2  

Unigene Cluster for SIAH3:

Siah E3 ubiquitin protein ligase family member 3
Hs.368483  [show with all ESTs]
Unigene Representative Sequence: BC041372
1 Ensembl transcript including schematic representation, and UCSC links where relevant:
ENST00000400405(uc001vap.3)
miRNA
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Selected qRT-PCR Assays for microRNAs that regulate SIAH3 (see all 26):
hsa-miR-146b-3p hsa-miR-548k hsa-miR-29a hsa-miR-29c hsa-miR-767-5p hsa-miR-1265 hsa-miR-3613-3p hsa-miR-24
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Predesigned siRNA for gene silencing in human, mouse, rat SIAH3
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Primer
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  QuantiTect SYBR Green Assays in human, mouse, rat SIAH3
  QuantiFast Probe-based Assays in human, mouse, rat SIAH3

Additional mRNA sequence: 

AK125411.1 BC041372.2 

1 DOTS entry:

DT.95328392 

10 AceView cDNA sequences:

BC041372 AK125411 AW293773 AA442387 BU740990 AK096522 BI551601 NM_198849 
R40294 BM703200 

GeneLoc Exon Structure


(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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SIAH3 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS <intensity>2/3
CGAP TAG: GTGACACAAG
SIAH3 Expression
About this image

SIAH3 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

SIAH3 Protein Expression

SOURCE GeneReport for Unigene cluster: Hs.368483
    Custom PCR Arrays for SIAH3
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In Situ
Assay Products:
 

 
Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for SIAH3

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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This gene was present in the common ancestor of animals.

Orthologs for SIAH3 gene from Selected species (see all 14)    About this table
Organism Taxonomic
classification
Gene Description Human
Similarity
Orthology
Type
Details
mouse
(Mus musculus)
Mammalia Siah31 , 5 seven in absentia homolog 3 (Drosophila)1, 5 85.41(n)1
90.57(a)1
  14 (40.08 cM)5
3809181  NM_001128093.11  NP_001121565.11 
 754559825 
chicken
(Gallus gallus)
Aves SIAH31 siah E3 ubiquitin protein ligase family member 3 78.1(n)
80.17(a)
  418849  XM_417044.4  XP_417044.1 
lizard
(Anolis carolinensis)
Reptilia SIAH36
siah E3 ubiquitin protein ligase family member 3
49(a)
1 ↔ 1
3(18270308-18270966)
tropical clawed frog
(Xenopus tropicalis)
Amphibia siah31 siah E3 ubiquitin protein ligase family member 3 68.35(n)
72.57(a)
  100497552  XM_002940965.2  XP_002941011.1 
zebrafish
(Danio rerio)
Actinopterygii siah2l6
seven in absentia homolog 2 (Drosophila)-like
34(a)
1 → many
21(37619561-37678113) ENSDARG00000044381
fruit fly
(Drosophila melanogaster)
Insecta sina6
seven in absentia
35(a)
1 → many
3L(16846851-16851812)
worm
(Caenorhabditis elegans)
Secernentea siah-16
Protein SIAH-1 (siah-1) mRNA, complete cds
22(a)
1 → many
IV(3744045-3747655) WBGene00021369


ENSEMBL Gene Tree for SIAH3 (if available)
TreeFam Gene Tree for SIAH3 (if available)

(Paralogs according to 1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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Paralogs for SIAH3 gene
SIAH22  SIAH12  
2 SIMAP similar genes for SIAH3 using alignment to 1 protein entry:     SIAH3_HUMAN:
SIAH1    SIAH2

Find genes that share paralogs with SIAH3           About GenesLikeMe


1 Pseudogenes.org Pseudogene for SIAH3
PGOHUM00000243486


(SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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Selected SNPs for SIAH3 (see all 1810)    About this table                                 

Genomic DataTranscription Related DataAllele Frequencies
SNP IDValidClinical
significance
Chr 13 posSequence#AA
Chg
TypeMore#Allele
freq
PopTotal
sample
More
----------
rs1150700041,2
C,F--46353930(+) ATCTAT/ATCCTA 1 -- ds50011Minor allele frequency- A:0.03WA 118
rs1822256391,2
--46354096(+) CCACAC/TCTCCT 1 -- ds50010--------
rs1875396441,2
--46354139(+) GATACC/TGGTAA 1 -- ds50010--------
rs740722601,2
C,F--46354166(+) AAGTGC/TGCTGT 1 -- ds50012Minor allele frequency- T:0.50WA CSA 4
rs1925756361,2
--46354181(+) CTAGGC/TCTTCT 1 -- ds50010--------
rs73254111,2
C,F,H--46354229(+) ACTCTA/TGCACC 1 -- ds500121Minor allele frequency- N:0.00NS EA NA WA CSA 948
rs1833753291,2
--46354240(+) TTGGTA/GTGAAT 1 -- ds50010--------
rs1887800441,2
--46354263(+) TCCCAA/GCGTTC 1 -- ds50010--------
rs1436106141,2
--46354364(+) CAGCCA/CACCCA 1 -- ds50010--------
rs596579331,2
C--46354405(+) AGTGC-/TTTTTT 1 -- ut312Minor allele frequency- T:0.00NA CSA 4

HapMap Linkage Disequilibrium report for SIAH3 (46354405 - 46425871 bp)

Structural Variations
     Database of Genomic Variants (DGV) 5 variations for SIAH3:    About this table    
Variant IDTypeSubtypePubMed ID
dgv250e201CNV Deletion23290073
esv2747353CNV Deletion23290073
nsv1024CNV Loss18451855
nsv832597CNV Loss17160897
nsv527222CNV Gain19592680

Site Specific Mutation Identification with PCR Assays
SeqTarget long-range PCR primers for resequencing SIAH3
DNA2.0 Custom Variant and Variant Library Synthesis for SIAH3

(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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OMIM gene information: 615609    OMIM disorders: --


Find genes that share disorders with SIAH3           About GenesLikeMe

Genetic Association Database (GAD): SIAH3
Human Genome Epidemiology (HuGE) Navigator: SIAH3 (1 document)

Export disorders for SIAH3 gene to outside databases

(in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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PubMed articles for SIAH3 gene integrated from 10 sources:
(articles sorted by number of sources associating them with SIAH3)
    Utopia: connect your pdf to the dynamic
world of online information

  1. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PubMed id 20379614)1, 4 Rose J.E....Uhl G.R. (Mol. Med. 2010)
  2. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (Nat. Genet. 2004)
  3. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1, 3 Strausberg R.L....Marra M.A. (Proc. Natl. Acad. Sci. U.S.A. 2002)
  4. Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. (PubMed id 16344560)1 Kimura K.... Sugano S. (Genome Res. 2006)
  5. The DNA sequence and analysis of human chromosome 13. (PubMed id 15057823)2 Dunham A.... Ross M.T. (Nature 2004)
  6. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)2 Gerhard D.S.... Malek J. (Genome Res. 2004)

(in PubMed, OMIM, and NCBI Bookshelf)
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 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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Entrez Gene: 283514 HGNC: 30553 AceView: LOC283514 Ensembl:ENSG00000215475 euGenes: HUgn283514
ECgene: SIAH3 H-InvDB: SIAH3

(According to HUGE)
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  --

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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NameDescription
PharmGKB entry for SIAH3 Pharmacogenomics, SNPs, Pathways

(Patent information from GeneIP,
Licensable technologies from WIS Yeda, Salk, Tufts,
IP news from LifeMap Sciences, Inc.)
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Patent Information for SIAH3 gene:
Search GeneIP for patents involving SIAH3

GeneCards and IP:
Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, eBioscience, antibodies-online, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
Advanced Cell Diagnostics, Animal models from genOway)
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 Block miRNA regulation of human, mouse, rat SIAH3 using miScript Target Protectors SeqTarget long-range PCR primers for resequencing SIAH3
 DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat SIAH3 Predesigned siRNA for gene silencing in human, mouse, rat SIAH3
 QuantiFast Probe-based Assays in human, mouse, rat SIAH3 QuantiTect SYBR Green Assays in human, mouse, rat SIAH3
 Custom PCR Arrays for SIAH3 Search Chromatin IP Primers for SIAH3
 Pre-validated RT2 qPCR Primer Assay in human, mouse, rat SIAH3  Search GeneGlobe Interaction Network for SIAH3
 Regulatory tfbs in SIAH3 promoter
 GenScript Custom Purified and Recombinant Proteins Services for SIAH3 GenScript cDNA clones with any tag delivered in your preferred vector for SIAH3
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 Vector-based siRNA and miRNA, Ready for Transfection Gene Mutant Library, Variants up to 10^11
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 Browse Sino Biological Proteins
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 Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

 Novus Biologicals
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 See all of Abcam's Antibodies, Kits and Proteins for SIAH3
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 Browse ProSpec Recombinant Proteins
 Proteins for SIAH3
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 Browse ESI BIO Cell Lines and PureStem Progenitors for SIAH3
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 Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for SIAH3
 Browse SwitchGear 3'UTR luciferase reporter plasmids for SIAH3
 Browse SwitchGear Promoter luciferase reporter plasmids for SIAH3
 ThermoFisher Antibodies for SIAH3
 Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat SIAH3
 Browse compounds at ApexBio
 Search Addgene for plasmids for SIAH3
  Search eBioscience for proteins for SIAH3
  Search eBioscience for elisas for SIAH3
  eBioscience FlowRNA Probe Sets
 genOway: Develop your customized and physiologically relevant rodent model for SIAH3
 antibodies-online antibodies for SIAH3 (13 products)
 antibodies-online kits for SIAH3
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 antibodies-online proteins for SIAH3
       
GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014 , 21 Aug 2014 , 8 Sep 2014 , 7 Oct 2014

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