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SIAH1 Gene

protein-coding   GIFtS: 62
GCID: GC16M048391

Siah E3 Ubiquitin Protein Ligase 1

(Previous name: seven in absentia homolog 1 (Drosophila))
Microbiology & Infectious Diseases Congress
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(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Siah E3 Ubiquitin Protein Ligase 11 2     EC 6.3.2.-3
Seven In Absentia Homolog 12 3     HUMSIAH3
Seven In Absentia Homolog 1 (Drosophila)1     Siah-13
SIAH1A2     Siah-1a3
E3 Ubiquitin-Protein Ligase SIAH12     EC 6.3.28
siah-1a2     

External Ids:    HGNC: 108571   Entrez Gene: 64772   Ensembl: ENSG000001964707   OMIM: 6022125   UniProtKB: Q8IUQ43   

Export aliases for SIAH1 gene to outside databases

Previous GC identifers: GC16M038809 GC16M048443 GC16M048131 GC16M048172 GC16M046951 GC16M034285


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for SIAH1 Gene:
This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3
ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity
of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the
regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in
several additional transcript variants, some encoding different isoforms and others that have not been fully
characterized. (provided by RefSeq, Jul 2008)

GeneCards Summary for SIAH1 Gene:
SIAH1 (siah E3 ubiquitin protein ligase 1) is a protein-coding gene. Diseases associated with SIAH1 include balanitis, and hypoxia. GO annotations related to this gene include ubiquitin-protein ligase activity and protein C-terminus binding. An important paralog of this gene is SIAH3.

UniProtKB/Swiss-Prot: SIAH1_HUMAN, Q8IUQ4
Function: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of
target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a
thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity
either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3
complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in
transcription regulation (ELL2, MYB, POU2AF1, PML and RBBP8), a cell surface receptor (DCC), the cell-surface
receptor-type tyrosine kinase FLT3, the cytoplasmic signal transduction molecules (KLF10/TIEG1 and NUMB), an
antiapoptotic protein (BAG1), a microtubule motor protein (KIF22), a protein involved in synaptic vesicle
function in neurons (SYP), a structural protein (CTNNB1) and SNCAIP. Confers constitutive instability to HIPK2
through proteasomal degradation. It is thereby involved in many cellular processes such as apoptosis, tumor
suppression, cell cycle, axon guidance, transcription regulation, spermatogenesis and TNF-alpha signaling. Has
some overlapping function with SIAH2. Induces apoptosis in cooperation with PEG3. Upon nitric oxid (NO)
generation that follows apoptotic stimulation, interacts with S-nitrosylated GAPDH, mediating the translocation
of GAPDH to the nucleus. GAPDH acts as a stabilizer of SIAH1, facilitating the degradation of nuclear proteins

Gene Wiki entry for SIAH1 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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Regulatory elements:
   Regulatory transcription factor binding sites in the SIAH1 gene promoter:
         MEF-2A   Sp1   MEF-2   aMEF-2   E47   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 3): SIAH1 promoter sequence
   Search Chromatin IP Primers for SIAH1

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat SIAH1


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 16q12.1   Ensembl cytogenetic band:  16q12.1   HGNC cytogenetic band: 16q12.1

SIAH1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
SIAH1 gene location

GeneLoc information about chromosome 16         GeneLoc Exon Structure

GeneLoc location for GC16M048391:  view genomic region     (about GC identifiers)

Start:
48,390,275 bp from pter      End:
48,482,313 bp from pter
Size:
92,039 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
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UniProtKB/Swiss-Prot: SIAH1_HUMAN, Q8IUQ4 (See protein sequence)
Recommended Name: E3 ubiquitin-protein ligase SIAH1  
Size: 282 amino acids; 31123 Da
Subunit: Homodimer. Interacts with group 1 glutamate receptors GRM1 and GRM5. Interacts with DAB1, which may
inhibit its activity. Interacts with UBE2E2. Interacts with PEG3. Interacts with GAPDH; leading to stabilize
SIAH1 (By similarity). Component of some large E3 complex composed of UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and
TBL1X. Interacts with UBE2I. Interacts with alpha-tubulin. Interacts with PEG10, which may inhibit its activity.
Interacts with KHDRBS3. Interacts with SNCAIP and HIPK2
6 PDB 3D structures from and Proteopedia for SIAH1:
2A25 (3D)        4C9Z (3D)        4CA1 (3D)        4I7B (3D)        4I7C (3D)        4I7D (3D)    
Secondary accessions: A0FKF3 O43269 Q49A58 Q92880
Alternative splicing: 3 isoforms:  Q8IUQ4-1   Q8IUQ4-2   Q8IUQ4-3   

Explore the universe of human proteins at neXtProt for SIAH1: NX_Q8IUQ4

Explore proteomics data for SIAH1 at MOPED

Post-translational modifications: 

  • Phosphorylated on Ser-19 by ATM and ATR. This phosphorylation disrupts SIAH1 interaction with HIPK2, and
    subsequent proteasomal degradation of HIPK21
  • Modification sites at neXtProt
  • Modification sites at PhosphoSitePlus
  • Selected DME Specific Peptides for SIAH1 (Q8IUQ4) (see all 15)
     PRSIHEG  YRLELNG  IRNLAME  SDCLVFD 


    See SIAH1 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (2 alternative transcripts): 
    NP_001006611.1  NP_003022.3  

    ENSEMBL proteins: 
     ENSP00000456421   ENSP00000369343   ENSP00000349156   ENSP00000378214   ENSP00000457415  
    Reactome Protein details: Q8IUQ4

    SIAH1 Human Recombinant Protein Products:

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    Browse recombinant and purified proteins available from Enzo Life Sciences
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    OriGene Protein Over-expression Lysate for SIAH1
    OriGene Custom MassSpec
    OriGene Custom Protein Services for SIAH1
    GenScript Custom Purified and Recombinant Proteins Services for SIAH1
    Novus Biologicals SIAH1 Proteins
    Novus Biologicals SIAH1 Lysates
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    ProSpec Recombinant Protein for SIAH1
    Cloud-Clone Corp. Proteins for SIAH1

    SIAH1 Antibody Products:

    Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
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    OriGene Antibodies for SIAH1
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    Novus Biologicals SIAH1 Antibodies
    Abcam antibodies for SIAH1
    Cloud-Clone Corp. Antibodies for SIAH1
    ThermoFisher Antibodies for SIAH1
    LSBio Antibodies in human, mouse, rat for SIAH1

    SIAH1 Assay Products:

    Browse Kits and Assays available from EMD Millipore
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    GenScript Custom Assay Services for SIAH1
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for SIAH1
    Cloud-Clone Corp. CLIAs for SIAH1


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    Selected InterPro protein domains (see all 7):
     IPR008974 TRAF-like
     IPR018121 7-in-absentia-prot_TRAF-dom
     IPR001841 Znf_RING
     IPR013083 Znf_RING/FYVE/PHD
     IPR004162 SINA_like

    Graphical View of Domain Structure for InterPro Entry Q8IUQ4

    ProtoNet protein and cluster: Q8IUQ4

    1 Blocks protein domain: IPB001841 Zn-finger

    UniProtKB/Swiss-Prot: SIAH1_HUMAN, Q8IUQ4
    Domain: The RING-type zinc finger domain is essential for ubiquitin ligase activity
    Domain: The SBD domain (substrate-binding domain) mediates the homodimerization and the interaction with substrate
    proteins. It is related to the TRAF family (By similarity)
    Similarity: Belongs to the SINA (Seven in absentia) family
    Similarity: Contains 1 RING-type zinc finger
    Similarity: Contains 1 SIAH-type zinc finger


    SIAH1 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: SIAH1_HUMAN, Q8IUQ4
    Function: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of
    target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a
    thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity
    either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3
    complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in
    transcription regulation (ELL2, MYB, POU2AF1, PML and RBBP8), a cell surface receptor (DCC), the cell-surface
    receptor-type tyrosine kinase FLT3, the cytoplasmic signal transduction molecules (KLF10/TIEG1 and NUMB), an
    antiapoptotic protein (BAG1), a microtubule motor protein (KIF22), a protein involved in synaptic vesicle
    function in neurons (SYP), a structural protein (CTNNB1) and SNCAIP. Confers constitutive instability to HIPK2
    through proteasomal degradation. It is thereby involved in many cellular processes such as apoptosis, tumor
    suppression, cell cycle, axon guidance, transcription regulation, spermatogenesis and TNF-alpha signaling. Has
    some overlapping function with SIAH2. Induces apoptosis in cooperation with PEG3. Upon nitric oxid (NO)
    generation that follows apoptotic stimulation, interacts with S-nitrosylated GAPDH, mediating the translocation
    of GAPDH to the nucleus. GAPDH acts as a stabilizer of SIAH1, facilitating the degradation of nuclear proteins
    Enzyme regulation: Inhibited by interaction with SNCAIP (isoform 2, but not isoform 1). May be inhibited by
    interaction with PEG10
    Induction: May be induced by p53/TP53, suggesting that it may be required to modulate p53/TP53 response. The
    relevance of such activity in vivo is however unclear and may not exist

         Genatlas biochemistry entry for SIAH1:
    Drosophila seven in absentia (sina) homolog 1,putatively involved in apoptosis and tumor suppression,also
    mediating through RING finger ubiquitin-conjugating enzyme (E2)-dependent ubiquitination

         Enzyme Numbers (IUBMB): EC 6.3.2.-1 EC 6.3.22

         Gene Ontology (GO): 4 molecular function terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004842ubiquitin-protein ligase activity ISS--
    GO:0005515protein binding IPI11483518
    GO:0008022protein C-terminus binding IPI11389840
    GO:0008270zinc ion binding ISS--
         
    SIAH1 for ontologies           About GeneDecksing


    Phenotypes:
         6 MGI mutant phenotypes (inferred from 1 allele(MGI details for Siah1a):
     cellular  endocrine/exocrine gland  growth/size/body  liver/biliary system  mortality/aging 
     reproductive system 

    SIAH1 for phenotypes           About GeneDecksing

    Animal Models:
         MGI mouse knock-out Siah1atm1Ddlb for SIAH1

       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for SIAH1
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for SIAH1

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for SIAH1
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for SIAH1

    miRNA
    Products:
        
    miRTarBase miRNAs that target SIAH1:
    hsa-mir-505-5p (MIRT037958), hsa-mir-424-5p (MIRT005887)

    Block miRNA regulation of human, mouse, rat SIAH1 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate SIAH1 (see all 42):
    hsa-miR-194* hsa-miR-140-5p hsa-miR-193a-3p hsa-miR-300 hsa-miR-15a hsa-miR-200a hsa-miR-374c hsa-miR-141
    SwitchGear 3'UTR luciferase reporter plasmidSIAH1 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for SIAH1
    Predesigned siRNA for gene silencing in human, mouse, rat SIAH1

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for SIAH1

    Clone
    Products:
         
    OriGene clones in human, mouse for SIAH1 (see all 13)
    OriGene ORF clones in mouse, rat for SIAH1
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): SIAH1 (NM_001006610)
    Sino Biological Human cDNA Clone for SIAH1
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for SIAH1
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat SIAH1

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for SIAH1
    Browse ESI BIO Cell Lines and PureStem Progenitors for SIAH1 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for SIAH1


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    SIAH1_HUMAN, Q8IUQ4: Cytoplasm. Nucleus. Note=Predominantly cytoplasmic. Partially nuclear
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus5
    cytosol4
    endosome2
    mitochondrion2
    plasma membrane2

    Gene Ontology (GO): Selected cellular component terms (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus ISS--
    GO:0005737cytoplasm TAS9334332
    GO:0005769early endosome IEA--
    GO:0005829cytosol TAS--
    GO:0005886plasma membrane IEA--

    SIAH1 for ontologies           About GeneDecksing


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for SIAH1 About   (see all 6)                                                                                              See pathways by source

    SuperPathContained pathways About
    1Parkin-Ubiquitin Proteasomal System pathway
    Proteolysis Role of Parkin in the Ubiquitin Proteasomal Pathway0.89
    Parkin-Ubiquitin Proteasomal System pathway0.89
    2L1CAM interactions
    Axon guidance0.63
    Developmental Biology0.63
    3p53 signaling pathway
    p53 signaling pathway0.41
    4Wnt signaling pathway (KEGG)
    Wnt signaling pathway0.40
    5Class I MHC mediated antigen processing and presentation
    Ubiquitin mediated proteolysis0.35

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    1 GeneGo (Thomson Reuters) Pathway for SIAH1
        Proteolysis Role of Parkin in the Ubiquitin-Proteasomal Pathway

    1 BioSystems Pathway for SIAH1
        Parkin-Ubiquitin Proteasomal System pathway


    1 Reactome Pathway for SIAH1
        Netrin-1 signaling


    3 Kegg Pathways  (Kegg details for SIAH1):
        p53 signaling pathway
    Ubiquitin mediated proteolysis
    Wnt signaling pathway

    UniProtKB/Swiss-Prot: SIAH1_HUMAN, Q8IUQ4
    Pathway: Protein modification; protein ubiquitination


    SIAH1 for pathways           About GeneDecksing

        Pathway & Disease-focused RT2 Profiler PCR Arrays including SIAH1: 

              p53 Signaling Pathway in human mouse rat
              Ubiquitin Ligases in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for SIAH1

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for SIAH1 (Q8IUQ41, 2, 3 ENSP000003491564) via UniProtKB, MINT, STRING, and/or I2D (see all 173)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    CACYBPQ9HB712, 3, ENSP000003566524MINT-18767 MINT-18768 I2D: score=4 STRING: ENSP00000356652
    ATN1P542592, 3, ENSP000003490764MINT-2863180 I2D: score=3 STRING: ENSP00000349076
    HIPK3Q9H4222, 3MINT-61746 I2D: score=3 
    POU2AF1Q166331, 3EBI-747107,EBI-943588 I2D: score=3 
    ATXN7O152651, 3EBI-747107,EBI-708350 I2D: score=1 
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 16):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006508proteolysis TAS9334332
    GO:0006511ubiquitin-dependent protein catabolic process IDA--
    GO:0006915apoptotic process TAS9403064
    GO:0007049cell cycle IEA--
    GO:0007275multicellular organismal development ----

    SIAH1 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
    About This Section

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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for SIAH1

    4 HMDB Compounds for SIAH1    About this table
    CompoundSynonyms CAS #PubMed Ids
    Adenosine monophosphate5'-AMP (see all 28)61-19-8--
    Adenosine triphosphate5'-(tetrahydrogen triphosphate) Adenosine (see all 24)56-65-5--
    Phosphoric acidacide phosphorique (FRENCH) (see all 20)7664-38-2--
    Pyrophosphate(4-)Diphosphoric acid ion (see all 10)14000-31-8--

    1 Novoseek inferred chemical compound relationship for SIAH1 gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    nitric oxide 0 6 16752048 (1), 15951807 (1)



    SIAH1 for compounds           About GeneDecksing



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
    About This Section

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    REFSEQ mRNAs for SIAH1 gene (2 alternative transcripts): 
    NM_001006610.1  NM_003031.3  

    8 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000565620 ENST00000568007(uc002efl.3) ENST00000380006(uc021ths.1)
    ENST00000356721(uc002efn.1) ENST00000394725(uc002efo.1) ENST00000563745
    ENST00000573005 ENST00000567973
    Congresses - knowledge worth sharing:
    European Congress of Clinical Microbiology and Infectious Diseases (ECCMID) 10 - 13 May 2014

    miRNA
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    Block miRNA regulation of human, mouse, rat SIAH1 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate SIAH1 (see all 42):
    hsa-miR-194* hsa-miR-140-5p hsa-miR-193a-3p hsa-miR-300 hsa-miR-15a hsa-miR-200a hsa-miR-374c hsa-miR-141
    SwitchGear 3'UTR luciferase reporter plasmidSIAH1 3' UTR sequence
    Inhib. RNA
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    OriGene RNAi products in human, mouse, rat for SIAH1
    Predesigned siRNA for gene silencing in human, mouse, rat SIAH1
    Clone
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    OriGene clones in human, mouse for SIAH1 (see all 13)
    OriGene ORF clones in mouse, rat for SIAH1
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): SIAH1 (NM_001006610)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for SIAH1
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat SIAH1
    Primer
    Products:
        
    OriGene qPCR primer pairs and template standards for SIAH1
    OriGene qSTAR qPCR primer pairs in human, mouse for SIAH1
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat SIAH1
      QuantiTect SYBR Green Assays in human, mouse, rat SIAH1
      QuantiFast Probe-based Assays in human, mouse, rat SIAH1

    Selected AceView cDNA sequences (see all 228):

    AW117503 BU521256 BF002338 D59180 AI368145 CR593197 AL046386 AW614409 
    BQ181813 CR607323 AW167318 CR614925 AA701951 AW593893 AA935716 BQ027303 
    BM670017 CR598465 BM699360 AI810587 AU136155 BM783469 AA885525 AW768860 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    SIAH1 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: CACAGTATTT
    SIAH1 Expression
    About this image

    SIAH1 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    SIAH1 Protein Expression

    UniProtKB/Swiss-Prot: SIAH1_HUMAN, Q8IUQ4
    Tissue specificity: Widely expressed at a low level. Down-regulated in advanced hepatocellular carcinomas

        Pathway & Disease-focused RT2 Profiler PCR Arrays including SIAH1: 
              p53 Signaling Pathway in human mouse rat
              Ubiquitin Ligases in human mouse rat

    Primer
    Products:
    OriGene qPCR primer pairs and template standards for SIAH1
    OriGene qSTAR qPCR primer pairs in human, mouse for SIAH1
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat SIAH1
    QuantiTect SYBR Green Assays in human, mouse, rat SIAH1
    QuantiFast Probe-based Assays in human, mouse, rat SIAH1
    In Situ
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for SIAH1

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of eukaryotes.

    Orthologs for SIAH1 gene from Selected species (see all 16)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Siah1a1 , 5 seven in absentia 1A1, 5 92.67(n)1
    99.65(a)1
      8 (42.10 cM)5
    204371  NM_009172.21  NP_033198.11 
     867239405 
    chicken
    (Gallus gallus)
    Aves SIAH11 seven in absentia homolog 1 (Drosophila) 90.31(n)
    99.65(a)
      374076  XM_414105.3  XP_414105.2 
    lizard
    (Anolis carolinensis)
    Reptilia SIAH16
    siah E3 ubiquitin protein ligase 1
    95(a)
    1 ↔ 1
    LGc(2571329-2572501)
    African clawed frog
    (Xenopus laevis)
    Amphibia Xl.20902 Xenopus laevis transcribed sequence with strong similarity more 84.33(n)    BU912024.1 
    zebrafish
    (Danio rerio)
    Actinopterygii idibd26352 idibd2635 77.31(n)   80955  BC045870.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta sina1 , 3 R7 cell fate commitment3
    seven in absentia1
    83(a)
    (best of 2)3
    64.56(n)1
    79.18(a)1
      3 73D33
    398841  NM_057377.41  NP_476725.11 
    worm
    (Caenorhabditis elegans)
    Secernentea Y37E11AR.23
    siah-11
    siah-11 67(a)3
    58.5(n)1
    63.4(a)1
      IV(3744137-3747213)3
    1771381  NM_068008.31  NP_500409.11 
    rice
    (Oryza sativa)
    Liliopsida Os07g06598001 Os07g0659800 46.27(n)
    36.47(a)
      4344172  NM_001067063.1  NP_001060528.1 


    ENSEMBL Gene Tree for SIAH1 (if available)
    TreeFam Gene Tree for SIAH1 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for SIAH1 gene
    SIAH32  SIAH22  
    2 SIMAP similar genes for SIAH1 using alignment to 3 protein entries:     SIAH1_HUMAN (see all proteins):
    SIAH2    SIAH3

    SIAH1 for paralogs           About GeneDecksing


    1 Pseudogenes.org Pseudogene for SIAH1
    PGOHUM00000241698


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for SIAH1 (see all 477)    About this table    
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 16 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs667615531,2
    C--48401707(+) CAAAA-/AGCTGA 2 -- us2k1 int11Minor allele frequency- A:0.50CSA 2
    rs1126613091,2
    C,F--48404579(+) AGGCGC/TGGTGG 1 -- int11Minor allele frequency- T:0.50CSA 2
    rs768887571,2
    C,F--48404690(+) CCAGCG/CTGGGC 1 -- int12Minor allele frequency- C:0.50WA CSA 4
    rs1999684071,2
    C--48415502(+) ATTACA/CAAGCC 1 -- int10--------
    rs1120331761,2
    C--48418901(+) GGCGAA/GCCGGC 1 -- int10--------
    rs1913249321,2
    --48418957(+) CGGGCA/GGCCCG 1 -- int10--------
    rs1178231281,2
    C,F--49409177(+) GAAACT/CGGGGC 2 -- ds50011Minor allele frequency- C:0.03NA 120
    rs721507481,2
    C--49409291(+) AGTGA-/ATTT  
            
    ATTAT
    2 -- ds50010--------
    rs740179311,2
    C,F--49409382(+) TGGGCA/GAGAGG 2 -- ds50013Minor allele frequency- G:0.07WA CSA 122
    rs348521501,2
    C--49409442(+) TGTGAT/-TTAGA 2 -- ds50011Minor allele frequency- -:0.00CSA 2

    HapMap Linkage Disequilibrium report for SIAH1 (48390275 - 48482313 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 3 variations for SIAH1:    About this table    
    Variant IDTypeSubtypePubMed ID
    esv26254CNV Loss19812545
    nsv833219CNV Gain17160897
    dgv888e1CNV Complex17122850

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing SIAH1
    DNA2.0 Custom Variant and Variant Library Synthesis for SIAH1

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 602212    OMIM disorders: --

    14 diseases for SIAH1:    About MalaCards
    balanitis    hypoxia    parkinson's disease    herpes simplex
    ataxia    ischemia    nasopharyngitis    hepatitis b
    hepatocellular carcinoma    hepatitis    malaria    breast cancer
    neuronitis    leukemia


    SIAH1 for disorders           About GeneDecksing


    Congresses - knowledge worth sharing:
    Alzheimer's & Parkinson's Diseases Congress (ADPD) 18 - 22 March 2015

    2 Novoseek inferred disease relationships for SIAH1 gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    parkinson disease 45.8 2 16752048 (2)
    tumors 0 3 20144232 (3)

    Genetic Association Database (GAD): SIAH1
    Human Genome Epidemiology (HuGE) Navigator: SIAH1 (1 document)

    Export disorders for SIAH1 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for SIAH1 gene, integrated from 10 sources (see all 116):
    (articles sorted by number of sources associating them with SIAH1)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Characterization of human homologs of the Drosophila seven in absentia (sina) gene. (PubMed id 9403064)1, 2, 3 Hu G.... Fearon E.R. (Genomics 1997)
    2. Mammalian homologs of seven in absentia regulate DCC via the ubiquitin-proteasome pathway. (PubMed id 9334332)1, 2, 3 Hu G.... Fearon E.R. (Genes Dev. 1997)
    3. Mutation analysis of the seven in absentia homolog 1 (SIAH1) gene in Parkinson's disease. (PubMed id 16752048)1, 4, 9 Franck T....Riess O. (J Neural Transm 2006)
    4. SIAH1 targets the alternative splicing factor T-STAR for degradation by the proteasome. (PubMed id 15163637)1, 2, 9 Venables J.P.... Elliott D.J. (Hum. Mol. Genet. 2004)
    5. Structural analysis of Siah1-Siah-interacting protein interactions and insights into the assembly of an E3 ligase multiprotein complex. (PubMed id 16085652)1, 2, 9 Santelli E.... Matsuzawa S. (J. Biol. Chem. 2005)
    6. Structural analysis of Siah1 and its interactions with Siah- interacting protein (SIP). (PubMed id 12421809)1, 2, 9 Matsuzawa S.... Ely K.R. (J. Biol. Chem. 2003)
    7. Modulation of transforming growth factor beta (TGFbeta)/Smad transcriptional responses through targeted degradation of TGFbeta- inducible early gene-1 by human seven in absentia homologue. (PubMed id 12072443)1, 2, 9 Johnsen S.A....Spelsberg T.C. (J. Biol. Chem. 2002)
    8. Siah-1 mediates a novel beta-catenin degradation pathway linking p53 to the adenomatous polyposis coli protein. (PubMed id 11389840)1, 2, 9 Liu J.... Matsunami N. (Mol. Cell 2001)
    9. Siah-1 facilitates ubiquitination and degradation of synphilin-1. (PubMed id 14506261)1, 2, 9 Nagano Y.... Matsumoto M. (J. Biol. Chem. 2003)
    10. The ubiquitin ligase Siah1 controls ELL2 stability and formation of super elongation complexes to modulate gene transcription. (PubMed id 22483617)1, 2 Liu M.... Zhou Q. (Mol. Cell 2012)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 6477 HGNC: 10857 AceView: SIAH1 Ensembl:ENSG00000196470 euGenes: HUgn6477
    ECgene: SIAH1 Kegg: 6477 H-InvDB: SIAH1

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for SIAH1 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for SIAH1 Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for SIAH1 gene:
    Search GeneIP for patents involving SIAH1

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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