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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

SCAMP2 Gene

protein-coding   GIFtS: 56
GCID: GC15M075136

secretory carrier membrane protein 2

  Search for SCAMP2
in our new
 Human Malady Compendium 
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(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Secretory Carrier Membrane Protein 21 2 3
Secretory Carrier-Associated Membrane Protein 22

External Ids:    HGNC: 105641   Entrez Gene: 100662   Ensembl: ENSG000001404977   OMIM: 6069125   UniProtKB: O151273   

Export aliases for SCAMP2 gene to outside databases

Previous GC identifers: GC15P070892 GC15P068180 GC15M072711 GC15M072853 GC15M072924 GC15M051934


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for SCAMP2:
This gene product belongs to the SCAMP family of proteins which are secretory carrier membrane proteins. They function
as carriers to the cell surface in post-golgi recycling pathways. Different family members are highly related products
of distinct genes, and are usually expressed together. These findings suggest that the SCAMPs may function at the same
site during vesicular transport rather than in separate pathways. (provided by RefSeq, Jul 2008)

UniProtKB/Swiss-Prot: SCAM2_HUMAN, O15127
Function: Functions in post-Golgi recycling pathways. Acts as a recycling carrier to the cell surface

Gene Wiki entry for SCAMP2


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000015.9  NC_018926.1  NT_010194.17  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the SCAMP2 gene promoter:
         STAT5B   STAT1   STAT4   STAT1beta   STAT5A   STAT1alpha   CHOP-10   STAT2   STAT3   RSRFC4   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidSCAMP2 promoter sequence
   Search SABiosciences Chromatin IP Primers for SCAMP2

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat SCAMP2


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 15q23-q25   Ensembl cytogenetic band:  15q24.1   HGNC cytogenetic band: 15q23-q25

SCAMP2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
SCAMP2 gene location

GeneLoc information about chromosome 15         GeneLoc Exon Structure

GeneLoc location for GC15M075136:  view genomic region     (about GC identifiers)

Start:
75,136,071 bp from pter      End:
75,165,706 bp from pter
Size:
29,636 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: SCAM2_HUMAN, O15127 (See protein sequence)
Recommended Name: Secretory carrier-associated membrane protein 2  
Size: 329 amino acids; 36649 Da
Subunit: Interacts with SLC6A4 and SLC9A7
Subcellular location: Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Recycling endosome
membrane; Multi-pass membrane protein
Secondary accessions: B2RDF0 Q9BQE8

Explore the universe of human proteins at neXtProt for SCAMP2: NX_O15127

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_O15127

  • SCAMP2 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_005688.2  
    ENSEMBL proteins: 
     ENSP00000268099   ENSP00000455377   ENSP00000455963   ENSP00000454741   ENSP00000454464  
     ENSP00000457890   ENSP00000457564  

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    Uscn Proteins for SCAMP2

    Gene Ontology (GO): 5/7 cellular component terms (GO ID links to tree view) (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IDA--
    GO:0005730NOT nucleolus IDA--
    GO:0005794Golgi apparatus IDA--
    GO:0016021integral to membrane IDA15840657
    GO:0032588trans-Golgi network membrane IDA15840657


    SCAMP2 for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    SCAMP2 for domains           About GeneDecksing

    1 InterPro domain/family:
     IPR007273 SCAMP

    Graphical View of Domain Structure for InterPro Entry O15127

    ProtoNet protein and cluster: O15127

    1 Blocks protein family: IPB007273 SCAMP

    UniProtKB/Swiss-Prot: SCAM2_HUMAN, O15127
    Similarity: Belongs to the SCAMP family


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: SCAM2_HUMAN, O15127
    Function: Functions in post-Golgi recycling pathways. Acts as a recycling carrier to the cell surface

         Genatlas biochemistry entry for SCAMP2:
    secretory carrier membrane protein 2,widely distributed as component of post-Golgi membranes,involved in cell surface
    recycling

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    Gene Ontology (GO): 1 molecular function term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515protein binding IPI16870614


    SCAMP2 for ontologies           About GeneDecksing


    1 GenomeRNAi human phenotype for SCAMP2:
     Increased circadian rhythm amp 


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways  About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1Arf6 trafficking events
    Arf6 trafficking events1.00
    2Monoamine Transport
    Monoamine Transport1.00


    2 BioSystems Pathways for SCAMP2 
        Monoamine Transport
    Arf6 trafficking events


    Interactions:

        SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for SCAMP2

    STRING Interaction Network Preview (showing 5 interactants - click image to see 7)

    5/10 Interacting proteins for SCAMP2 (O151272, 3 ENSP000002680994) via UniProtKB, MINT, STRING, and/or I2D (see all 10)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    YWHAZP631042, 3, ENSP000003095034MINT-3320159 I2D: score=1 STRING: ENSP00000309503
    SLC9A7Q96T833, ENSP000003303204I2D: score=1 STRING: ENSP00000330320
    SNAP23O001613, ENSP000002496474I2D: score=2 STRING: ENSP00000249647
    ARIH2O953763I2D: score=4 
    ST3GAL3Q112033I2D: score=3 
    About this table

    Gene Ontology (GO): 2 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006892post-Golgi vesicle-mediated transport TAS9378760
    GO:0015031protein transport IDA15840657


    SCAMP2 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
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    Browse Tocris compounds for SCAMP2
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    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
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    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for SCAMP2 gene: 
    NM_005697.3  

    Unigene Cluster for SCAMP2:

    Secretory carrier membrane protein 2
    Hs.458917  [show with all ESTs]
    Unigene Representative Sequence: AK096794
    12 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000268099(uc002azb.1) ENST00000566480(uc002aza.1) ENST00000563663
    ENST00000563829 ENST00000569251 ENST00000569904 ENST00000567638 ENST00000565345
    ENST00000562363 ENST00000564529 ENST00000566557 ENST00000569124

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    hsa-miR-106a hsa-miR-339-5p hsa-miR-519a hsa-miR-124 hsa-miR-93 hsa-miR-506 hsa-miR-526b* hsa-miR-17
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    Additional cDNA sequence: 

    AF005038.2 AK096376.1 AK096794.1 AK291884.1 AK315516.1 BC001376.2 BC004385.1 CR456800.1 

    16 DOTS entries:

    DT.80101357  DT.100846303  DT.100846298  DT.100804573  DT.92446044  DT.100846299  DT.100846300  DT.95360564 
    DT.100712409  DT.92052870  DT.92446049  DT.121078419  DT.121078423  DT.121625057  DT.95374355  DT.91887875 

    24/424 AceView cDNA sequences (see all 424):

    BI821658 AU076895 AA479955 BI752383 BU150304 AW571867 CD672397 BU732626 
    BE218423 AI143247 BP429360 AI364067 AA298947 BM794467 AA311509 NM_005697 
    BE904711 BC001376 CR600693 BU621542 AI375955 AI282443 CA487703 AI674218 

    GeneLoc Exon Structure

    5/6 Alternative Splicing Database (ASD) splice patterns (SP) for SCAMP2 (see all 6)    About this scheme

    ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3 ^ 4a · 4b · 4c ^ 5a · 5b ^ 6 ^ 7 ^ 8a · 8b · 8c ^ 9 ^ 10
    SP1:                          -                 -     -           -                             -               
    SP2:                          -                 -     -           -                 -                           
    SP3:                                                              -                                             
    SP4:                          -                 -     -                                                         
    SP5:                          -                                                                                 


    ECgene alternative splicing isoforms for SCAMP2

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    SCAMP2 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: ATGGTGGTGG

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See SCAMP2 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for SCAMP2

    SOURCE GeneReport for Unigene cluster: Hs.458917

    UniProtKB/Swiss-Prot: SCAM2_HUMAN, O15127
    Tissue specificity: Widely expressed

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    In Situ
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of eukaryotes.

    Orthologs for SCAMP2 gene from 8/25 species (see all 25)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    chicken
    (Gallus gallus)
    Aves SCAMP21 secretory carrier membrane protein 2 77.27(n)
    80.19(a)
      769853  XM_001233171.2  XP_001233172.1 
    lizard
    (Anolis carolinensis)
    Reptilia SCAMP26
    --
    72(a)
    1 ↔ 1
    GL343492.1(585525-598215)
    African clawed frog
    (Xenopus laevis)
    Amphibia Xl.242702 Xenopus laevis transcribed sequence with moderate similarity more 73.47(n)    CF289275.1 
    zebrafish
    (Danio rerio)
    Actinopterygii ceacam12 carcinoembryonic antigen-related cell adhesion molecule more 75.09(n)   394169  BC055274.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta Scamp3 synaptic vesicle exocytosis 39(a)   13C3   --
    worm
    (Caenorhabditis elegans)
    Secernentea scm-16
    SCAMP (synaptic vesicle protein) homolog family me...
    33(a)
    1 → many
    I(1862614-1872308)
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons AT1G320501 secretory carrier-associated membrane protein 4 42.8(n)
    29.66(a)
      840097  NM_102939.3  NP_174485.1 
    rice
    (Oryza sativa)
    Liliopsida Os04g05958001 hypothetical protein 45.31(n)
    33.85(a)
      4336849  NM_001060269.1  NP_001053734.1 


    ENSEMBL Gene Tree for SCAMP2 (if available)
    TreeFam Gene Tree for SCAMP2 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for SCAMP2 gene
    SCAMP32  SCAMP52  SCAMP42  
    4 SIMAP similar genes for SCAMP2 using alignment to 8 protein entries:     SCAM2_HUMAN (see all proteins):
    SCAMP5    SCAMP4    SCAMP1    SCAMP3

    SCAMP2 for paralogs           About GeneDecksing


    2 Pseudogenes.org Pseudogenes for SCAMP2
    PGOHUM00000262424 PGOHUM00000262460


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/467 NCBI SNPs in SCAMP2 are shown (see all 467    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 15 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1925384431,2
    --75136766(+) CCATCC/GCCTGC 2 -- ds5001 us2k10--------
    rs1464914791,2
    --75136778(+) GGAGGA/GTGCAG 2 -- us2k1 ds50010--------
    rs1410770411,2
    --75136797(+) AGGGCA/TATTAA 2 -- ds5001 us2k10--------
    rs8731921,2
    C,F,A,--75137004(-) CTTAGA/GCACAG 2 -- ds5001 us2k16Minor allele frequency- G:0.03MN NA EA 666
    rs10443551,2
    C,--75137064(-) TTCTGC/TCAGGG 2 -- us2k1 ds50015Minor allele frequency- T:0.13MN NA EA 337
    rs1431786621,2
    --75137069(+) GCAGAA/CAGCTC 2 -- us2k1 ds50010--------
    rs747354451,2
    --75137123(+) GGGGTA/GTCTGT 2 -- ds5001 us2k10--------
    rs1409354611,2
    --75137141(+) CAGGAC/TGAAGA 2 -- us2k1 ds50010--------
    rs1497109891,2
    --75137217(+) CTTTG-/TTTTTT 2 -- us2k1 ut310--------
    rs110171,2
    C,F,H,--75137252(-) ACAGGG/AAATTT 2 -- us2k1 ut31 ese33Minor allele frequency- A:0.01NS NA 400

    HapMap Linkage Disequilibrium report for SCAMP2 (75136071 - 75165706 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 1 variation for SCAMP2
         1 Inversion: 37170

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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    SCAMP2 for disorders           About MalaCards

    SCAMP2 for disorders           About GeneDecksing

    OMIM gene information: 606912    OMIM disorders: --

    1 disease from the University of Copenhagen DISEASES database for SCAMP2:
    Bartter disease
    Human Genome Epidemiology (HuGE) Navigator: SCAMP2 (1 document)

    Export disorders for SCAMP2 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for SCAMP2 gene, integrated from 9 sources (see all 23):
    (articles sorted by number of sources associating them with SCAMP2)
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    1. Three mammalian SCAMPs (secretory carrier membrane proteins) are highly related products of distinct genes having similar subcellular distributions. (PubMed id 9378760)1, 2, 3 Singleton D.R.... Castle J.D. (1997)
    2. Subcellular redistribution of the serotonin transporter by secretory carrier membrane protein 2. (PubMed id 16870614)1, 2, 9 Muller H.K....Haase J. (2006)
    3. Secretory carrier membrane proteins interact and regulate trafficking of the organellar (Na+,K+)/H+ exchanger NHE7. (PubMed id 15840657)1, 2 Lin P.J.... Numata M. (2005)
    4. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    5. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
    6. Secretory Carrier Membrane Protein 2 Regulates Cell-surface Targeting of Brain-enriched Na+/H+ Exchanger NHE5. (PubMed id 19276089)1, 9 Diering G.H....Numata M. (2009)
    7. Secretory carrier membrane protein SCAMP2 and phosphatidylinositol 4,5-bisphosphate interactions in the regulation of dense core vesicle exocytosis. (PubMed id 17713930)1, 9 Liao H....Castle D. (2007)
    8. Proteome-wide identification of ubiquitylation sites b y conjugation of engineered lysine-less ubiquitin. (PubMed id 22053931)1 Oshikawa K....Nakayama K.I. (2012)
    9. Modulation of the dopamine transporter by interaction with Secretory Carrier Membrane Protein 2. (PubMed id 21295544)1 Fjorback A.W....Wiborg O. (2011)
    10. Initial characterization of the human central proteome. (PubMed id 21269460)2 Burkard T.R.... Colinge J. (2011)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 10066 HGNC: 10564 AceView: SCAMP2 Ensembl:ENSG00000140497 euGenes: HUgn10066
    ECgene: SCAMP2 H-InvDB: SCAMP2

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for SCAMP2 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for SCAMP2 gene:
    Search GeneIP for patents involving SCAMP2

    GeneCards and IP:
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