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SARM1 Gene

protein-coding   GIFtS: 50

GC17P023723
sterile alpha and TIR motif containing 1
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
FLJ36296 2
KIAA0524 1, 2, 3
SAMD2 1, 2
SARM 1, 2, 3
Descriptions
Sterile alpha and Armadillo repeat protein 3
Tir-1 homolog 3
sterile alpha and HEAT/Armadillo motif protein, ortholog of
Drosophila 2
sterile alpha and TIR motif containing 1 2
External Ids
HGNC: 170741
Entrez Gene: 230982
UniProtKB: Q6SZW13
Ensembl: ENSG000000041397
Search outside databases for aliases for SARM1 gene

Previous GC identifers: GC17P026550 GC17P026844

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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UniProtKB/Swiss-Prot: SARM1_HUMAN, Q6SZW1
Function: Involved in innate immnune response. Acts as a negative regulator of
TICAM1/TRIF-dependent Toll-like receptor signaling by inhibiting induction of TLR3- and
TLR4-dependent genes. Specifically blocks TICAM1/TRIF-dependent transcription-factor activation
and gene induction, without affecting the MYD88-dependent pathway or non-TLR signaling. Negative
regulator of NF-kappa-B and IRF activation

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the SARM1 gene  

Entrez Gene cytogenetic band: 17q11   Ensembl cytogenetic band:  17q11.2   HGNC cytogenetic band: 17q11

SARM1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 17         GeneLoc Exon Structure

GeneLoc location for GC17P023723:     (about GC identifiers)

Start:
23,723,114 bp from pter
End:
23,752,192 bp from pter
Size:
29,079 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000017.9  NT_010799.14  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: SARM1_HUMAN, Q6SZW1 (See protein sequence)
Recommended Name: Sterile alpha and TIR motif-containing protein 1  
Size: 724 amino acids; 79388 Da
Subunit: Interacts with TICAM1/TRIF and thereby interferes with TICAM1/TRIF function
Subcellular location: Cytoplasm (By similarity)
Caution: Was initially reported to contain ARM repeats (PubMed:11386760). Such repeats are however
not predicted by any detection method
Secondary accessions: O60277 Q7LGG3 Q9NXY5
Alternative splicing: 2 isoforms:  Q6SZW1-1   Q6SZW1-2   

REFSEQ proteins: NP_055892.2  

ENSEMBL proteins: 
ENSP00000003834 ENSP00000368351 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (SARM)
Browse Abnova for Human Recombinant Proteins
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

4 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005737 cytoplasm IEA--
GO:0005874 microtubule IEA--
GO:0031224 intrinsic to membrane IEA--
GO:0031315 extrinsic to mitochondrial outer membrane IEA--
About this table

Antibodies for SARM1: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for SARM1
Browse R&D Systems for Antibodies
Antibodies from Abcam (SARM), each with their AbpromiseSM
Browse Abnova for Monoclonal and Polyclonal Antibodies
Search Novus for antibodies for SARM1

Assays for SARM1: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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4 InterPro domains/families:
 IPR011989 ARM-like
 IPR001660 SAM
 IPR011510 SAM_2
 IPR000157 Toll-Interleukin_rcpt


   GeneDecks  SARM1 for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry Q6SZW1

ProtoNet protein and cluster: Q6SZW1

2 Blocks protein families:
IPB001660 Sterile alpha motif SAM
IPB011510 Sterile alpha motif homology 2


UniProtKB/Swiss-Prot: SARM1_HUMAN, Q6SZW1
Similarity: Contains 2 SAM (sterile alpha motif) domains
Similarity: Contains 1 TIR domain

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (SARM1)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (SARM1)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_015077

              Applied Biosystems Silencer® siRNAs for SARM1

              Sigma-Aldrich siRNA and siRNA Panels for SARM1  
                     Sigma-Aldrich shRNA for SARM1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Browse Clone Ranger at Invitrogen for clones
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_015077
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_015077
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_015077 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_015077

UniProtKB/Swiss-Prot: SARM1_HUMAN, Q6SZW1
Function: Involved in innate immnune response. Acts as a negative regulator of
TICAM1/TRIF-dependent Toll-like receptor signaling by inhibiting induction of TLR3- and
TLR4-dependent genes. Specifically blocks TICAM1/TRIF-dependent transcription-factor activation
and gene induction, without affecting the MYD88-dependent pathway or non-TLR signaling. Negative
regulator of NF-kappa-B and IRF activation

2 MGI mutant phenotypes (inferred from 1 allele(MGI details for Sarm1):

cellularimmune system

2 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0004888 transmembrane receptor activity IEA--
GO:0005515 protein binding IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section



5/14 Interacting proteins for SARM1 (ENSP000003683513) via UniProtKB, MINT, and/or STRING (see all 14 )
InteractantInteraction Details
GeneCardExternal ID(s)
ARENSP000003638223STRING (score=.953)
TICAM1ENSP000002482443STRING (score=.93)
IRF3ENSP000003101273STRING (score=.905)
TLR3ENSP000002967953STRING (score=.904)
TLR4ENSP000003630893STRING (score=.858)
About this table

4 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0007165 signal transduction IEA--
GO:0009749 response to glucose stimulus IEA--
GO:0042981 regulation of apoptosis IEA--
GO:0045087 innate immune response IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for SARM1

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (SARM1)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (SARM1)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_015077

              Sigma-Aldrich siRNA and siRNA Panels for SARM1  
                     Sigma-Aldrich shRNA for SARM1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_015077  

REFSEQ mRNAs for SARM1 gene: 

NM_015077.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_015077  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_015077
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_015077
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_015077 

Additional cDNA sequence: 

AB011096.1 AF086363.1 AJ290445.1 AK026397.1 AK093615.1 AK302038.1 AK314609.1 AY444166.1 
BC040429.1 BC122860.1 CR616327.1 CR626592.1 

8 DOTS entries:

DT.95373041  DT.99968346  DT.40240655  DT.100782897  DT.100025807  DT.120988319  DT.99941606  DT.120988299 

24/102 AceView cDNA sequences (see all 102 ):

CR626592 BM791687 BQ068917 BM795235 BX090713 AA281198 AW770507 CR623769 
BU178839 AA729916 BX106603 CR616327 AA281090 AA568670 CD511988 NM_015077 
BG911443 AJ290445 BX483880 AL534755 BF339050 R70607 BU151384 AY444166 

highest scoring ESTs for SARM1:

AB011096 AA281090 AA350494 AI934275 AW953254 BG820141 BM672700 BM791687 BQ368533 BQ575644 

Unigene Cluster for SARM1:

Sterile alpha and TIR motif containing 1
Hs.719354  [show with all ESTs]
Unigene Representative Sequence: NM_015077


GeneLoc Exon Structure

5/8 Alternative Splicing Database (ASD) splice patterns (SP) for SARM1 (see all 8 )

ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5a · 5b · 5c ^ 6a · 6b ^ 7 ^ 8 ^ 9a · 9b · 9c ^ 10a · 10b · 10c · 10d ^ 11a · 11b · 11c ^ 12a · 12b · 12c
SP1:                                                        -     -     -     -           -                                   -                     
SP2:                          -     -                       -     -     -     -                                                                     
SP3:                                                        -           -     -           -                                                         
SP4:                                                        -     -     -                 -                                   -                     
SP5:                                                              -     -     -                                               -                     

About this scheme

ECgene alternative splicing isoforms for SARM1

2 Ensembl transcripts including schematic representations:
ENST00000003834  ENST00000379061  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

SARM1 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for SARM1

1 / 2 / 3

5 probe-sets matching SARM1 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

41707_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 AB011096 1.00 0.90 0.95 1

213257_at2, 3 U133-A 1 1.00 1.00 -- -- AJ290445 0.80 0.88 0.84 1

213259_s_at2, 3 U133-A 1 1.00 1.00 -- -- AI937297 0.60 0.75 0.68 1

213257_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

213259_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
About this table
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: --

SOURCE GeneReport for Unigene cluster: Hs.719354

Expression variation in blood from EXPOLDB for SARM1

UniProtKB/Swiss-Prot: SARM1_HUMAN, Q6SZW1
Tissue specificity: Predominantly expressed in kidney and liver. Expressed at lower level in
placenta

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for SARM1 gene from 5/8 species (see all 8 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
SARM11   -- sterile alpha and TIR motif containing 1 92.22(n)
95.72(a)
491165  XM_548285.2  XP_548285.2 
rat
(Rattus norvegicus)
Sarm11   -- sterile alpha and TIR motif containing 1 88.13(n)
93.49(a)
287545  XM_213404.4  XP_213404.2 
mouse
(Mus musculus)
Sarm11, 5 11 (45.00 cM)5
sterile alpha and HEAT/Armadillo motif containing 11, 5 88.18(n)1
94.04(a)1
2378681  NM_172795.21  NP_766383.21 
 AC0023245  AK0441135  (see all 17)
chicken
(Gallus gallus)
SARM11   -- sterile alpha and TIR motif containing 1 72.37(n)
72.84(a)
417568  XM_415814.2  XP_415814.2 
zebrafish
(Danio rerio)
sarm11   -- sterile alpha and TIR motif containing 1 64.56(n)
63.85(a)
403143  XM_001344371.1  XP_001344407.1 
About this table        Species with no ortholog for SARM1

ENSEMBL Gene Tree for SARM1
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

  --
(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/113 NCBI SNPs in SARM1 are shown (see all 113 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 77)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 17 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
--
rs116581941,2
C23732677(+) TGCTTC/TCGCTG 1 R/W mis10--------
rs99142391,2
A,C,F,H23734040(+) TCCCTA/GCAATA 1 -- int13Minor allele frequency- G:0.15EU EA 296
rs20279931,2
A,C,F,H23731073(-) gaaatA/Ccaaaa 1 -- int16Minor allele frequency- C:0.38EA EU WA 600
rs22697411,2
A,C,F,H23733572(-) agactC/Taaaag 1 -- int14Minor allele frequency- T:0.01EU EA WA 376
rs65050791,2
A,C,F,H23742304(+) gcttgA/Tcatca 1 -- int14Minor allele frequency- T:0.33EU EA WA 416
--
rs72166571,2
A,C,F,H23736516(+) GCCTCG/ACCGTG 1 -- int14Minor allele frequency- A:0.15EU EA WA 412
rs22697421,2
A,C,F,H23731722(-) GCCAGC/GCACTG 1 -- int14Minor allele frequency- G:0.17EU EA WA 418
rs98942601,2
A,F,H23743752(+) gactcC/Tcgagg 1 -- int14Minor allele frequency- T:0.17EU EA WA 420
rs72253721,2
A,F,H23734658(+) GGCAGG/ATCATT 1 -- int14Minor allele frequency- A:0.01EU EA WA 418
--
rs9676451,2
A,C,F23738097(-) gaacaA/Gcctgg 1 -- int11Minor allele frequency- G:0.49MN 184
About this table

HapMap Linkage Disequilibrium images for SARM1 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 607732

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/12 PubMed articles for SARM1 gene (see all 12 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 23098 HGNC: 17074 AceView: SARM1 Ensembl:ENSG00000004139 euGenes: HUgn23098
ECgene: SARM1 H-InvDB: SARM1
(According to HUGE)
About This Section

HUGE: KIAA0524
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
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  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for SARM1:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



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 Predesigned and custom siRNAs for SARM1 Antibodies for SARM1
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for SARM1 Browse small molecules at Sigma-Aldrich
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