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Aliases for SARAF Gene

Aliases for SARAF Gene

  • Store-Operated Calcium Entry Associated Regulatory Factor 2 3 5
  • SOCE-Associated Regulatory Factor 2 3 4
  • Transmembrane Protein 66 2 3 4
  • HBV X-Transactivated Gene 3 Protein 3 4
  • HBV XAg-Transactivated Protein 3 3 4
  • TMEM66 3 4
  • XTP3 3 4
  • Store-Operated Calcium Entry-Associated Regulatory Factor 3
  • Testicular Secretory Protein Li 59 3
  • SARAF Short Isoform 3
  • SARAF Long Isoform 3
  • Protein FOAP-7 4
  • HSPC035 3
  • FOAP-7 3

External Ids for SARAF Gene

Previous HGNC Symbols for SARAF Gene

  • TMEM66

Summaries for SARAF Gene

GeneCards Summary for SARAF Gene

SARAF (Store-Operated Calcium Entry Associated Regulatory Factor) is a Protein Coding gene.

UniProtKB/Swiss-Prot for SARAF Gene

  • Negative regulator of store-operated Ca(2+) entry (SOCE) involved in protecting cells from Ca(2+) overfilling. In response to cytosolic Ca(2+) elevation after endoplasmic reticulum Ca(2+) refilling, promotes a slow inactivation of STIM (STIM1 or STIM2)-dependent SOCE activity: possibly act by facilitating the deoligomerization of STIM to efficiently turn off ORAI when the endoplasmic reticulum lumen is filled with the appropriate Ca(2+) levels, and thus preventing the overload of the cell with excessive Ca(2+) ions.

Gene Wiki entry for SARAF Gene

No data available for Entrez Gene Summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for SARAF Gene

Genomics for SARAF Gene

Regulatory Elements for SARAF Gene

Enhancers for SARAF Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH08F030075 1.2 FANTOM5 Ensembl ENCODE 28.5 +6.6 6608 2.4 FEZF1 ZNF316 CBX5 GATA2 NFE2 MAFK ZNF362 MAFG DPF2 ARID1B LEPROTL1 SARAF RNU6-1218P MBOAT4 ENSG00000272256
GH08F030087 1.5 FANTOM5 Ensembl ENCODE 21.7 -9.4 -9391 9.6 HDGF PKNOX1 ARID4B SIN3A YY1 FOS NFYC REST TBX21 MEF2D LEPROTL1 SARAF MBOAT4
GH08F030079 0.5 ENCODE 16.7 +0.9 885 5.0 HDGF PKNOX1 MLX ARID4B SIN3A FEZF1 DMAP1 ZNF2 YY1 SLC30A9 LEPROTL1 WRN SARAF TAGLN2P2 FAM183CP LOC105379354 GTF2E2 ENSG00000272256 RNU6-1218P
GH08F030097 1.2 FANTOM5 ENCODE 15 -16.8 -16804 4.6 PKNOX1 ATF1 CREB3L1 MLX ZNF133 ARID4B DMAP1 YY1 FOS SP5 LEPROTL1 SARAF MBOAT4 GC08M030097 RPS15AP24
GH08F030102 0.8 Ensembl ENCODE 12.2 -20.4 -20397 2.0 ZNF362 HLF CEBPA PRDM1 ZNF644 SARAF UBXN8 GC08M030097 RPS15AP24
- Elite enhancer/Elite enhancer-gene association Download Table
Download GeneHancer data dump

Enhancers around SARAF on UCSC Golden Path with GeneCards custom track

Promoters for SARAF Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00001389095 1708 4201 HDGF PKNOX1 MLX ARID4B SIN3A FEZF1 DMAP1 ZNF2 YY1 SLC30A9

Genomic Location for SARAF Gene

Chromosome:
8
Start:
30,063,012 bp from pter
End:
30,083,208 bp from pter
Size:
20,197 bases
Orientation:
Minus strand

Genomic View for SARAF Gene

Genes around SARAF on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
SARAF Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for SARAF Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for SARAF Gene

Proteins for SARAF Gene

  • Protein details for SARAF Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q96BY9-SARAF_HUMAN
    Recommended name:
    Store-operated calcium entry-associated regulatory factor
    Protein Accession:
    Q96BY9
    Secondary Accessions:
    • B3KQQ4
    • B7Z9J1
    • D3DSU7
    • H9MHJ8
    • H9MHJ9
    • Q53HE8
    • Q9UNZ3
    • Q9Y683

    Protein attributes for SARAF Gene

    Size:
    339 amino acids
    Molecular mass:
    36975 Da
    Quaternary structure:
    • Interacts with STIM1.
    SequenceCaution:
    • Sequence=AAD44487.1; Type=Frameshift; Positions=28; Evidence={ECO:0000305};

    Alternative splice isoforms for SARAF Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for SARAF Gene

Post-translational modifications for SARAF Gene

  • Ubiquitination at Lys 43, Lys 65, Lys 79, Lys 86, Lys 106, Lys 109, Lys 145, and Lys 326
  • Modification sites at PhosphoSitePlus

No data available for DME Specific Peptides for SARAF Gene

Domains & Families for SARAF Gene

Protein Domains for SARAF Gene

InterPro:
ProtoNet:

Graphical View of Domain Structure for InterPro Entry

Q96BY9

UniProtKB/Swiss-Prot:

SARAF_HUMAN :
  • The cytoplasmic C-terminal region mediates interaction with STIM1, while the N-terminal lumenal region mediates regulation of SOCE activity.
  • Belongs to the SARAF family.
Domain:
  • The cytoplasmic C-terminal region mediates interaction with STIM1, while the N-terminal lumenal region mediates regulation of SOCE activity.
Family:
  • Belongs to the SARAF family.
genes like me logo Genes that share domains with SARAF: view

No data available for Gene Families for SARAF Gene

Function for SARAF Gene

Molecular function for SARAF Gene

UniProtKB/Swiss-Prot Function:
Negative regulator of store-operated Ca(2+) entry (SOCE) involved in protecting cells from Ca(2+) overfilling. In response to cytosolic Ca(2+) elevation after endoplasmic reticulum Ca(2+) refilling, promotes a slow inactivation of STIM (STIM1 or STIM2)-dependent SOCE activity: possibly act by facilitating the deoligomerization of STIM to efficiently turn off ORAI when the endoplasmic reticulum lumen is filled with the appropriate Ca(2+) levels, and thus preventing the overload of the cell with excessive Ca(2+) ions.

Gene Ontology (GO) - Molecular Function for SARAF Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005515 protein binding IPI 22464749
genes like me logo Genes that share ontologies with SARAF: view
genes like me logo Genes that share phenotypes with SARAF: view

Animal Model Products

Inhibitory RNA Products

Clone Products

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for SARAF Gene

Localization for SARAF Gene

Subcellular locations from UniProtKB/Swiss-Prot for SARAF Gene

Endoplasmic reticulum membrane; Single-pass type I membrane protein. Note=Translocates to the endoplasmic reticulum-plasma membrane (ER-PM) region in a STIM1-dependent manner following cytosolic Ca(2+) elevation.
Isoform 2: Endoplasmic reticulum membrane; Single-pass type I membrane protein.

Gene Ontology (GO) - Cellular Components for SARAF Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005783 endoplasmic reticulum IEA --
GO:0005789 endoplasmic reticulum membrane IEA --
GO:0016020 membrane IEA --
GO:0016021 integral component of membrane IEA --
GO:0030176 integral component of endoplasmic reticulum membrane IDA 22464749
genes like me logo Genes that share ontologies with SARAF: view

No data available for Subcellular locations from COMPARTMENTS for SARAF Gene

Pathways & Interactions for SARAF Gene

SuperPathways for SARAF Gene

No Data Available

Gene Ontology (GO) - Biological Process for SARAF Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006810 transport IEA --
GO:0006811 ion transport IEA --
GO:0006816 calcium ion transport IEA --
GO:2001256 regulation of store-operated calcium entry IDA 22464749
genes like me logo Genes that share ontologies with SARAF: view

No data available for Pathways by source and SIGNOR curated interactions for SARAF Gene

Transcripts for SARAF Gene

Inhibitory RNA Products

Clone Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for SARAF Gene

ExUns: 1a · 1b · 1c · 1d · 1e · 1f · 1g ^ 2 ^ 3 ^ 4a · 4b · 4c · 4d ^ 5 ^ 6a · 6b · 6c ^ 7a · 7b · 7c ^ 8a · 8b · 8c ^ 9a · 9b · 9c ·
SP1: - - - - -
SP2: - - - - - -
SP3: - - - - - - - - -
SP4: -
SP5: - - -
SP6: -
SP7: - - -
SP8: - - - - -
SP9: - - - - - - - - - - - - -
SP10: -
SP11:

ExUns: 9d · 9e
SP1:
SP2:
SP3:
SP4:
SP5:
SP6:
SP7:
SP8:
SP9:
SP10:
SP11:

Relevant External Links for SARAF Gene

GeneLoc Exon Structure for
SARAF
ECgene alternative splicing isoforms for
SARAF

Expression for SARAF Gene

mRNA expression in normal human tissues for SARAF Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for SARAF Gene

This gene is overexpressed in Bone (48.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for SARAF Gene



NURSA nuclear receptor signaling pathways regulating expression of SARAF Gene:

SARAF

mRNA Expression by UniProt/SwissProt for SARAF Gene:

Q96BY9-SARAF_HUMAN
Tissue specificity: Highly expressed in macrophages.
genes like me logo Genes that share expression patterns with SARAF: view

Primer Products

No data available for mRNA differential expression in normal tissues and Protein tissue co-expression partners for SARAF Gene

Orthologs for SARAF Gene

This gene was present in the common ancestor of animals.

Orthologs for SARAF Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia TMEM66 34 35
  • 99.31 (n)
dog
(Canis familiaris)
Mammalia TMEM66 34 35
  • 87.46 (n)
cow
(Bos Taurus)
Mammalia TMEM66 34 35
  • 83.82 (n)
mouse
(Mus musculus)
Mammalia Tmem66 34 35
  • 82.09 (n)
Saraf 16
rat
(Rattus norvegicus)
Mammalia Tmem66 34
  • 79.4 (n)
platypus
(Ornithorhynchus anatinus)
Mammalia -- 35
  • 52 (a)
OneToMany
-- 35
  • 50 (a)
OneToMany
oppossum
(Monodelphis domestica)
Mammalia TMEM66 35
  • 49 (a)
OneToOne
chicken
(Gallus gallus)
Aves TMEM66 35
  • 39 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia TMEM66 35
  • 50 (a)
OneToOne
African clawed frog
(Xenopus laevis)
Amphibia Xl.2119 34
zebrafish
(Danio rerio)
Actinopterygii tmem66 35
  • 40 (a)
OneToOne
worm
(Caenorhabditis elegans)
Secernentea T06D8.9 34 35
  • 48.85 (n)
sea squirt
(Ciona savignyi)
Ascidiacea -- 35
  • 34 (a)
OneToOne
Species where no ortholog for SARAF was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)

Evolution for SARAF Gene

ENSEMBL:
Gene Tree for SARAF (if available)
TreeFam:
Gene Tree for SARAF (if available)

Paralogs for SARAF Gene

Pseudogenes.org Pseudogenes for SARAF Gene

genes like me logo Genes that share paralogs with SARAF: view

No data available for Paralogs for SARAF Gene

Variants for SARAF Gene

Sequence variations from dbSNP and Humsavar for SARAF Gene

SNP ID Clin Chr 08 pos Sequence Context AA Info Type
rs10046786 -- 30,069,062(+) acagg(A/G)gtcAG intron-variant
rs10090403 -- 30,085,339(+) attct(G/T)ttata upstream-variant-2KB
rs10090988 -- 30,079,888(+) CCCAC(A/G)CCCCA intron-variant, upstream-variant-2KB
rs10099336 -- 30,083,595(+) AATAC(A/G)TTGTC upstream-variant-2KB
rs10106729 -- 30,081,314(+) TCTTT(C/T)ATAGT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for SARAF Gene

Variant ID Type Subtype PubMed ID
esv2656771 CNV deletion 23128226
esv2736824 CNV deletion 23290073
esv2736825 CNV deletion 23290073
esv2736826 CNV deletion 23290073
esv3543288 CNV deletion 23714750
nsv831277 CNV gain 17160897

Variation tolerance for SARAF Gene

Residual Variation Intolerance Score: 63.3% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for SARAF Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
SARAF

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for SARAF Gene

Disorders for SARAF Gene

Relevant External Links for SARAF

Atlas of Genetics and Cytogenetics in Oncology and Haematology:
SARAF

No disorders were found for SARAF Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for SARAF Gene

Publications for SARAF Gene

  1. SARAF inactivates the store operated calcium entry machinery to prevent excess calcium refilling. (PMID: 22464749) Palty R. … Reuveny E. (Cell 2012) 2 3 4 64
  2. Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells. (PMID: 11042152) Zhang Q.-H. … Chen Z. (Genome Res. 2000) 2 3 4 64
  3. The STIM1 CTID domain determines access of SARAF to SOAR to regulate Orai1 channel function. (PMID: 23816623) Jha A. … Muallem S. (J. Cell Biol. 2013) 2 3 64
  4. Signal sequence and keyword trap in silico for selection of full- length human cDNAs encoding secretion or membrane proteins from oligo- capped cDNA libraries. (PMID: 16303743) Otsuki T. … Isogai T. (DNA Res. 2005) 3 4 64
  5. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T. … Sugano S. (Nat. Genet. 2004) 3 4 64

Products for SARAF Gene

Sources for SARAF Gene

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