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SAR1A Gene

protein-coding   GIFtS: 59

GC10M071580
SAR1 homolog A (S. cerevisiae)
(Previous names: SAR1a gene homolog (S. cerevisiae) 1, SAR1a gene homolog 1 (S. cerevisiae) )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: SARA1)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
OTTHUMP00000019743 2
OTTHUMP00000019744 2
OTTHUMP00000019745 2
OTTHUMP00000019748 2
OTTHUMP00000060603 2
SAR1 1, 2, 3
SARA 3
SARA1 2, 3, 5
Sara 1, 2
masra2 2
Descriptions
COPII-associated small GTPase 3
SAR1 gene homolog A 2
SAR1 homolog A (S. cerevisiae) 2
SAR1a gene homolog (S. cerevisiae) 1 1
SAR1a gene homolog 1 2
SAR1a gene homolog 1 (S. cerevisiae) 1
External Ids
HGNC: 105341
Entrez Gene: 566812
UniProtKB: Q9NR313
Ensembl: ENSG000000793327
Search outside databases for aliases for SAR1A gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: SAR1A_HUMAN, Q9NR31
Function: Involved in transport from the endoplasmic reticulum to the Golgi apparatus (By
similarity). Required to maintain SEC16A localization at discrete locations on the ER membrane
perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane
forms an organized scaffold defining endoplasmic reticulum exit sites (ERES)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the SAR1A gene  

Entrez Gene cytogenetic band: 10q22.1   Ensembl cytogenetic band:  10q22.1   HGNC cytogenetic band: 10q22.1

SAR1A Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 10         GeneLoc Exon Structure

GeneLoc location for GC10M071580:     (about GC identifiers)

Start:
71,579,966 bp from pter
End:
71,600,285 bp from pter
Size:
20,320 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000010.9  NT_008583.16  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: SAR1A_HUMAN, Q9NR31 (See protein sequence)
Recommended Name: GTP-binding protein SAR1a  
Size: 198 amino acids; 22367 Da
Subcellular location: Endoplasmic reticulum (By similarity). Golgi apparatus (By similarity)
PDB structures from and Proteopedia :
2GAO (3D)    

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins (2 alternative transcripts): 
    NP_001136120.1  NP_064535.1  


    ENSEMBL proteins: 
    ENSP00000362339 ENSP00000378471 ENSP00000362333 ENSP00000362335 ENSP00000362338 ENSP00000362336 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (SAR1)
    Human Recombinant Proteins from Abnova (SAR1A)
                  OriGene Purified Recombinant Human Protein: SAR1A 

    4 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005622 intracellular IEA--
    GO:0005737 cytoplasm NAS--
    GO:0005794 Golgi apparatus IEA--
    GO:0016529 sarcoplasmic reticulum IEA--
    About this table

    Antibodies for SAR1A: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for SAR1A
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (SAR1), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (SAR1A)
    Novus Biologicals Antibodies for SAR1A

    Assays for SAR1A: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    3 InterPro domains/families:
     IPR006687 SAR1_GTP_bd
     IPR006689 ARF/SAR
     IPR005225 Small_GTP_bd


       GeneDecks  SAR1A for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9NR31

    ProtoNet protein and cluster: Q9NR31

    2 Blocks protein families:
    IPB001806 Transforming protein P21 RAS signature
    IPB006687 GTP-binding protein SAR1


    UniProtKB/Swiss-Prot: SAR1A_HUMAN, Q9NR31
    Similarity: Belongs to the small GTPase superfamily. SAR1 family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (SAR1A)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (SAR1A)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_020150

                  Applied Biosystems Silencer® siRNAs for SAR1A

                  Sigma-Aldrich siRNA for SAR1A  
                         Sigma-Aldrich shRNA for SAR1A  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for SAR1A
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001142648
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001142648
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_001142648 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_020150

    UniProtKB/Swiss-Prot: SAR1A_HUMAN, Q9NR31
    Function: Involved in transport from the endoplasmic reticulum to the Golgi apparatus (By
    similarity). Required to maintain SEC16A localization at discrete locations on the ER membrane
    perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane
    forms an organized scaffold defining endoplasmic reticulum exit sites (ERES)

    3 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000166 nucleotide binding IEA--
    GO:0003924 GTPase activity NAS--
    GO:0005525 GTP binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Millipore Pathway for SAR1A
     Clathrin-dependent protein traffic

       GeneDecks  SAR1A for the pathways selected above  
    About GeneDecksing


    5/10 Interacting proteins for SAR1A (ENSP000003623363) via UniProtKB, MINT, and/or STRING (see all 10 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    GABRG2ENSP000003490003STRING (score=.899)
    GAD1ENSP000003509283STRING (score=.899)
    MGAMENSP000003164313STRING (score=.899)
    TGFB1ENSP000002219303STRING (score=.899)
    TGFB2ENSP000003558963STRING (score=.899)
    About this table

    3 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006886 intracellular protein transport IEA--
    GO:0006888 ER to Golgi vesicle-mediated transport NAS--
    GO:0016192 vesicle-mediated transport IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for SAR1A

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (SAR1A)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (SAR1A)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_020150

                  Sigma-Aldrich siRNA for SAR1A  
                         Sigma-Aldrich shRNA for SAR1A  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_001142648  NM_020150  

    REFSEQ mRNAs for SAR1A gene (2 alternative transcripts): 

    NM_001142648.1   NM_020150.4   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_001142648  NM_020150  

                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001142648
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001142648
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_001142648 

    Additional cDNA sequence: 

    AF086166.1 AF087897.1 AF217959.1 AF261717.1 AF274026.1 AF318066.1 AK025857.1 AK074257.1 
    AK298591.1 AK312469.1 AL136724.1 AY008268.1 BC003658.2 CR533492.1 

    12 DOTS entries:

    DT.95259145  DT.97862695  DT.87016923  DT.121261144  DT.92433004  DT.100042097  DT.121261116  DT.100792928 
    DT.91733074  DT.95100927  DT.95112223  DT.101958209 

    24/356 AceView cDNA sequences (see all 356 ):

    BQ941426 BM974100 AI200872 AA290688 BQ954254 AV712082 CA392755 AI086814 
    CD676038 AI470172 AI201809 CA425843 W60913 BE855644 BQ086264 AA938282 
    BM974996 AA938505 BX281349 BM556837 AF086166 BM453963 BQ008447 AA769518 

    highest scoring ESTs for SAR1A:

    AA046557 AA287759 AA334525 AA354348 AF087897 AF217959 AF261717 AF274026 AI143121 AW406969 

    Unigene Cluster for SAR1A:

    SAR1 homolog A (S. cerevisiae)
    Hs.499960  [show with all ESTs]
    Unigene Representative Sequence: NM_001142648


    GeneLoc Exon Structure

    3 Alternative Splicing Database (ASD) splice patterns (SP) for SAR1A

    ExUns: 1a · 1b ^ 2 ^ 3a · 3b ^ 4 ^ 5 ^ 6a · 6b ^ 7
    SP1:                          -                                 
    SP2:                          -                                 
    SP3:                                                            

    About this scheme

    ECgene alternative splicing isoforms for SAR1A

    6 Ensembl transcripts including schematic representations:
    ENST00000373242  ENST00000395026  ENST00000373236  ENST00000373238  ENST00000373241  ENST00000373239  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    SAR1A expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for SAR1A

    1 / 2 / 3

    9 probe-sets matching SAR1A gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    41450_at2, 3 U95-A 1 1.00 1.00 0.83 1.06 AI553878 0.60 1.00 0.82 1

    45748_at2, 3 U95-B 1 1.00 1.00 0.61 1.09 AI143121 0.80 1.00 0.91 1

    41451_s_at2, 3 U95-A 6 1.00 0.77 0.87 0.85 W28498 0.60 1.00 0.82 1

    210790_s_at2, 3 U133-A 1 1.00 1.00 -- -- BC003658 0.80 1.00 0.91 1

    201542_at2, 3 U133-A 1 1.00 1.00 -- -- AY008268 0.80 1.00 0.91 1

    201543_s_at2, 3 U133-A 6 1.00 0.54 -- -- NM_020150 0.60 1.00 0.82 1

    210790_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    201542_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    201543_s_at2 U133Plus2 6 1.00 0.54 -- -- -- -- -- -- --
    GeneDecks  SAR1A for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: AATTATGACT

    SOURCE GeneReport for Unigene cluster: Hs.499960

    Expression variation in blood from EXPOLDB for SAR1A

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for SAR1A gene from 5/18 species (see all 18 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    SAR1A1   -- SAR1 homolog A (S. cerevisiae) 94.78(n)
    98.99(a)
    479237  XM_536379.2  XP_536379.1 
    cow
    (Bos taurus)
    SAR1A1   -- SAR1 homolog A (S. cerevisiae) 95.29(n)
    98.99(a)
    517171  NM_001034521.1  NP_001029693.1 
    rat
    (Rattus norvegicus)
    Sar1a1   -- SAR1 homolog A (S. cerevisiae) 93.27(n)
    99.49(a)
    361842  NM_001007739.1  NP_001007740.1 
    mouse
    (Mus musculus)
    Sar1a1, 5 105
    SAR1 gene homolog A (S. cerevisiae)1, 5 93.1(n)1
    99.49(a)1
    202241  NM_009120.21  NP_033146.11 
     AK0035385  AK0054955  (see all 18)
    chicken
    (Gallus gallus)
    SAR1A1   -- SAR1 homolog A (S. cerevisiae) 85.19(n)
    97.47(a)
    423711  XM_421589.2  XP_421589.2 
    About this table        Species with no ortholog for SAR1A

    ENSEMBL Gene Tree for SAR1A
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for SAR1A gene
    SAR1B2  ARL92  ARL102  

    Pseudogenes
    3 related pseudogenes


    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/149 NCBI SNPs in SAR1A are shown (see all 149 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 62)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 10 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    --
    rs105861,2
    A,C,F,H71580038(+) CTTTTC/GCTGAA 2 -- ut31 ese37Minor allele frequency- G:0.04NA EU EA WA 562
    --
    rs79196471,2
    A,C,F,H71580894(+) GATGCT/CGGTGC 2 -- ut31 ese311Minor allele frequency- C:0.03NA EU EA WA 982
    --
    rs10467471,2
    A,C,F,H71580636(-) TCATTC/TTTATT 2 -- ut3116Minor allele frequency- T:0.17MN NA EU EA WA 1584
    --
    rs23946431,2
    A,C,F,H71581020(-) ttttGG/ATATGA 2 -- ut317Minor allele frequency- A:0.20EU EA WA NA 524
    rs158011,2
    A,C,F,H71580669(-) GGTGAG/AGGAGG 2 -- ut31 ese39Minor allele frequency- A:0.33MN NA EU EA WA 930
    rs38126921,2
    C,F,H71600208(+) CGGCTG/AGCTCG 2 -- ut51 ese35Minor allele frequency- A:0.07EA EU WA 1688
    rs125730971,2
    C,F,H71579909(+) CGGCAT/CTGTTC 1 -- ng519Minor allele frequency- C:0.47NA EU EA WA 792
    rs109991691,2
    C,F,H71601018(+) CTTGCC/TCTGGG 1 -- ng31 tfbs34Minor allele frequency- T:0.39EU EA WA 410
    rs38126931,2
    F,H71600249(+) CACCCG/TGGACC 2 -- ut51 ese34Minor allele frequency- T:0.01EU EA WA 368
    rs76531,2
    F,H71580322(-) TTTTTT/CCCCCT 2 -- ut31 ese3 trp35Minor allele frequency- C:0.02MN EU EA WA 602
    About this table

    HapMap Linkage Disequilibrium images for SAR1A (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 607691

    Human Genome Epidemiology Navigator: SAR1A (2 documents)

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/18 PubMed articles for SAR1A gene (see all 18 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 56681 HGNC: 10534 AceView: SARA1 Ensembl:ENSG00000079332 euGenes: HUgn56681
    ECgene: SAR1A H-InvDB: SAR1A
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for SAR1A:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
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     Predesigned and custom siRNAs for SAR1A Antibodies for SAR1A
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for SAR1A Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Custom Service for Rabbit Pab from Full-length Protein
      
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     Recombinant Proteins
    (SAR1)
     Antibodies (SAR1)
     Tagged/untagged cDNA clones
     Validated SYBR primer pairs
     Purified proteins from HEK293T cells
     Western blot validated antibodies
     shRNA in GFP-retroviral vector
     Search Tocris compounds for SAR1A




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for SAR1A

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