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SAP30 Gene

protein-coding   GIFtS: 56

GC04P174528
Sin3A-associated protein, 30kDa
(Previous names: sin3A-associated protein, 30kDa )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Descriptions
Sin3 corepressor complex subunit SAP30 2, 3
Sin3-associated polypeptide, 30 kDa 3
Sin3-associated polypeptide, 30kDa 2
Sin3A-associated protein, 30kDa 1, 2
histone deacetylase complex subunit SAP30 2
External Ids
HGNC: 105321
Entrez Gene: 88192
UniProtKB: O754463
Ensembl: ENSG000001641057
Search outside databases for aliases for SAP30 gene

Previous GC identifers: GC04P175212 GC04P174830 GC04P174987 GC04P174666

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for SAP30:
Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone
acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this
gene is a component of the histone deacetylase complex, which includes SIN3, SAP18, HDAC1, HDAC2,
RbAp46, RbAp48, and other polypeptides. This complex is active in deacetylating core histone
octamers, but inactive in deacetylating nucleosomal histones. A pseudogene of this gene is located
on chromosome 3. [provided by RefSeq]

UniProtKB/Swiss-Prot: SAP30_HUMAN, O75446
Function: Involved in the functional recruitment of the Sin3-histone deacetylase complex (HDAC) to
a specific subset of N-CoR corepressor complexes. Capable of transcription repression by N-CoR.
Active in deacetylating core histone octamers (when in a complex) but inactive in deacetylating
nucleosomal histones

Gene Wiki entry for SAP30

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the SAP30 gene  

Entrez Gene cytogenetic band: 4q34.1   Ensembl cytogenetic band:  4q34.1   HGNC cytogenetic band: 4q34.1

SAP30 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 4         GeneLoc Exon Structure

GeneLoc location for GC04P174528:     (about GC identifiers)

Start:
174,528,668 bp from pter
End:
174,535,258 bp from pter
Size:
6,591 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000004.10  NT_022792.17  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: SAP30_HUMAN, O75446 (See protein sequence)
Recommended Name: Histone deacetylase complex subunit SAP30  
Size: 220 amino acids; 23306 Da
Subunit: Interacts with SIN3A, SIN3B, HDAC2 and NCOR1 (By similarity). Component of the histone
deacetylase complex that includes at least SIN3A, HDAC1 and HDAC2. Interacts with HDAC1 and HCFC1
Subcellular location: Nucleus
PDB structures from and Proteopedia :
2KDP (3D)    

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_003855.1  

    ENSEMBL proteins: 
    ENSP00000296504 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (SAP30)
    Human Recombinant Proteins from Abnova (SAP30)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    2 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000118 histone deacetylase complex IDA9651585
    GO:0005634 nucleus IEA--
    About this table

    Antibodies for SAP30: 
    Browse Antibodies Central at Invitrogen
    Millipore Mono- and Polyclonal Antibodies for the study of SAP30
    Sigma-Aldrich Antibodies for SAP30
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (SAP30), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (SAP30)
    Novus Biologicals Antibodies for SAP30

    Assays for SAP30: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    ProtoNet protein and cluster: O75446

    UniProtKB/Swiss-Prot: SAP30_HUMAN, O75446
    Similarity: Belongs to the SAP30 family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (SAP30)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (SAP30)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_003864

                  Applied Biosystems Silencer® siRNAs for SAP30

                  Sigma-Aldrich siRNA for SAP30  
                         Sigma-Aldrich shRNA for SAP30  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for SAP30
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_003864
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_003864
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_003864 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_003864

    UniProtKB/Swiss-Prot: SAP30_HUMAN, O75446
    Function: Involved in the functional recruitment of the Sin3-histone deacetylase complex (HDAC) to
    a specific subset of N-CoR corepressor complexes. Capable of transcription repression by N-CoR.
    Active in deacetylating core histone octamers (when in a complex) but inactive in deacetylating
    nucleosomal histones

    2 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003714 transcription corepressor activity IDA9651585
    GO:0005515 protein binding IPI9651585 9874765 12670868
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    2 Invitrogen iPath™ Online BioAtlas - Pathways for SAP30 (Maps provided by GeneGo):
     Formation of Sin3A and NuRD complexes and their role in transcription regulation
     Ligand-Dependent Transcription of Retinoid-Target genes

       GeneDecks  SAP30 for the pathways selected above  
    About GeneDecksing

    5 Millipore Pathways for SAP30
     Development Notch Signaling Pathway
     Selected targets of Oct-3/4
     Transcription Sin3 and NuRD in transcription regulation
     Development NOTCH1-mediated pathway for NF-KB activity modulation
     Transcription Ligand-Dependent Transcription of Retinoid-Target genes

       GeneDecks  SAP30 for the pathways selected above  
    About GeneDecksing

    1 Sigma-Aldrich "Your Favorite Gene" Pathway for  SAP30  (Your Favorite Gene powered by Ingenuity) 
     DNA Methylation and Transcriptional Repression Signaling

       GeneDecks  SAP30 for the pathways selected above  
    About GeneDecksing

     Gene Network CentralTM Interacting Genes and Proteins Network for  SAP30 


    5/44 Interacting proteins for SAP30 (ENSP000002965043) via UniProtKB, MINT, and/or STRING (see all 44 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    HDAC1ENSP000003626493STRING (score=.999)
    RBBP4ENSP000003625923STRING (score=.999)
    SIN3AENSP000003536223STRING (score=.999)
    HDAC2ENSP000003576213STRING (score=.998)
    RBBP7ENSP000003694243STRING (score=.998)
    About this table

    2 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000122 negative regulation of transcription from RNA polymerase II promoter IEA--
    GO:0006355 regulation of transcription, DNA-dependent IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for SAP30

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (SAP30)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (SAP30)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_003864

                  Sigma-Aldrich siRNA for SAP30  
                         Sigma-Aldrich shRNA for SAP30  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_003864  

    REFSEQ mRNAs for SAP30 gene: 

    NM_003864.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_003864  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_003864
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_003864
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_003864 

    Additional cDNA sequence: 

    AF055993.1 BC016757.1 

    3 DOTS entries:

    DT.75165308  DT.65288648  DT.92424431 

    24/46 AceView cDNA sequences (see all 46 ):

    AA128116 AI138822 AW628758 AA564903 AI765148 BF726718 AI332505 AI936899 
    AW005627 AI025531 AI372048 AW236579 AI962241 AA746205 AI245057 CA434135 
    CD676900 BV167899 AI559899 AF055993 NM_003864 AA505291 BE044507 AI826705 

    highest scoring ESTs for SAP30:

    AF055993 AA026940 AA128116 AA128147 AI186178 AI283588 AI307385 AI417431 AI559899 AI634520 

    Unigene Cluster for SAP30:

    Sin3A-associated protein, 30kDa
    Hs.591715  [show with all ESTs]
    Unigene Representative Sequence: BC016757


    GeneLoc Exon Structure

    1 Ensembl transcript including schematic representation:
    ENST00000296504  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    SAP30 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for SAP30

    1 / 2 / 3

    6 probe-sets matching SAP30 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    40992_s_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 AF055993 1.00 1.00 1.00 1

    40993_r_at2, 3 U95-A 1 1.00 1.00 0.28 0.58 AF055993 1.00 1.00 1.00 1

    204900_x_at2, 3 U133-A 1 1.00 1.00 -- -- NM_003864 0.60 1.00 0.82 1

    204899_s_at2, 3 U133-A 1 1.00 1.00 -- -- BF247098 0.40 1.00 0.76 1

    204900_x_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    204899_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TAGAAATGTT

    SOURCE GeneReport for Unigene cluster: Hs.591715

    Expression variation in blood from EXPOLDB for SAP30

    UniProtKB/Swiss-Prot: SAP30_HUMAN, O75446
    Tissue specificity: Expressed in all tissues tested with highest levels in pancreas, ovary, PBL,
    spleen and thymus; lowest levels in brain, placenta, lung and kidney

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for SAP30 gene from 5/7 species (see all 7 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    SAP301   -- Sin3A-associated protein, 30kDa 95.15(n)
    97.73(a)
    607359  XM_843990.1  XP_849083.1 
    chimpanzee
    (Pan troglodytes)
    SAP301   -- Sin3A-associated protein, 30kDa 99.85(n)
    99.55(a)
    471351  XM_526733.2  XP_526733.2 
    cow
    (Bos taurus)
    SAP301   -- Sin3A-associated protein, 30kDa 92.12(n)
    97.73(a)
    781150  XM_001249429.1  XP_001249430.1 
    mouse
    (Mus musculus)
    Sap301, 5 8 (31.00 cM)5
    sin3 associated polypeptide1, 5 88.64(n)1
    95.91(a)1
    604061  NM_021788.11  NP_068560.11 
     AF0751365  AK0109285  (see all 9)
    chicken
    (Gallus gallus)
    SAP301   -- Sin3A-associated protein, 30kDa 80.19(n)
    83.02(a)
    422562  XM_420522.2  XP_420522.2 
    About this table        Species with no ortholog for SAP30

    ENSEMBL Gene Tree for SAP30
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for SAP30 gene
    SAP30L2  

    Pseudogenes
    1 related pseudogene


    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/40 NCBI SNPs in SAP30 are shown (see all 40 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 20)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 4 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs102136651,2
    A,C,F,H174535536(+) TCTTGT/GAATGC 1 -- ng314Minor allele frequency- G:0.10EU EA WA 414
    rs76963101,2
    C,F,H174526927(+) ttttgT/Gttttt 1 -- ng51 tfbs34Minor allele frequency- G:0.07EU EA WA 418
    rs9659311,2
    A,C,F,H174534258(-) ACAGAT/CGTAAG 1 -- int14Minor allele frequency- C:0.09EU NA EA 390
    rs9659321,2
    C,F,H174533996(-) CTTGCC/TTTTTC 1 -- int19Minor allele frequency- T:0.06MN EU EA WA 996
    rs100325071,2
    F,H174529533(+) CAAAGG/AGGACC 1 -- int14Minor allele frequency- A:0.01EU EA WA 416
    --
    rs117214521,2
    C174530298(+) TCGATA/GGCTCA 1 -- int10--------
    --
    rs76551551,2
    C174534501(+) caccaC/Ggccta 1 -- int10--------
    rs18023161,2
    --174528858(+) GGAGCA/GGCCAG 1 -- ut510--------
    rs122051,2
    --174535225(+) CTAAAG/TTAAAT 1 -- ut310--------
    --
    rs725316011,2
    --174527403(+) AAAAA-/AAMCCM 1 -- ng510--------
    About this table

    HapMap Linkage Disequilibrium images for SAP30 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 603378

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/35 PubMed articles for SAP30 gene (see all 35 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 8819 HGNC: 10532 AceView: SAP30 Ensembl:ENSG00000164105 euGenes: HUgn8819
    ECgene: SAP30 H-InvDB: SAP30
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    ATLAS Chromosomes in Cancer entry for SAP30 Genetics and Cytogenetics in Oncology and Haematology
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for SAP30:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
      Invitrogen Clones  Invitrogen Q-PCR Products
      Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for SAP30-related products

     Millipore Custom Antibody & Bulk Services
     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for SAP30 Antibodies for SAP30
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for SAP30 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Recombinant & Natural Proteins  

     Recombinant Proteins (SAP30)
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     Custom Service for Rabbit Pab from Full-length Protein
      
     Search for Antibodies & Assays

     Recombinant Proteins
    (SAP30)
     Antibodies (SAP30)
     Search OriGene for SAP30
     Search Tocris compounds for SAP30




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for SAP30

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