V3 here soon-try beta now


Gene Search (GeneCards Home)	\|	GeneCards Guide	\|	User Feedback	\|	Terms of Use	\|	Notice about third-party sites

SAMD8 Gene

protein-coding GIFtS: 44

GC10P076541

sterile alpha motif domain containing 8
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
About This Section

Aliases
FLJ25082 ^¹, ²
OTTHUMP00000019872 ^²
OTTHUMP00000060201 ^²
SMSR ^⁵
SMSr ^², ³

Descriptions
Sterile alpha motif domain-containing 8 ^³
sphingomyelin synthase related ^²
sterile alpha motif domain containing 8 ^²

External Ids
HGNC: 26320¹
Entrez Gene: 142891²
UniProtKB: Q96LT4³
Ensembl: ENSG00000156671⁷

Search outside databases for aliases for SAMD8 gene

Previous GC identifer: GC10P076216

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:

UCSC Golden Path with GeneCards custom track

Transcription factor binding sites upstream to the SAMD8 gene

Entrez Gene cytogenetic band: 10q22.2 Ensembl cytogenetic band: 10q22.2 HGNC cytogenetic band: 10q22.3

SAMD8 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 10 GeneLoc Exon Structure

GeneLoc location for GC10P076541: (about GC identifiers)

Start:	76,541,313 bp from pter
End:	76,606,613 bp from pter
Size:	65,301 bases
Orientation:	plus strand

RefSeq DNA sequence:

NC_000010.9 NT_008583.16

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: SAMD8_HUMAN, Q96LT4 (See protein sequence)

Recommended Name: Sphingomyelin synthase-related protein 1

Size: 415 amino acids; 48321 Da

Subcellular location: Membrane; Multi-pass membrane protein (Potential)

Sequence caution: Sequence=BAB71586.1; Type=Miscellaneous discrepancy; Note=Intron retention;

REFSEQ proteins: NP_653261.1

ENSEMBL proteins:

ENSP00000361775 ENSP00000361772

Human Recombinant Proteins

	Browse Drug Discovery Central at Invitrogen for human recombinant proteins
	Browse Purified and Recombinant Proteins at Millipore
	Browse Human Recombinant Proteins at Sigma-Aldrich
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Browse Abcam for Recombinant Proteins
	Human Recombinant Proteins from Abnova (SAMD8)

Browse Origene for full length recombinant human proteins expressed in human HEK293 cells

2 Gene Ontology (GO) cellular component terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0016020	membrane	IEA	--
GO:0016021	integral to membrane	NAS	14685263

About this table

Antibodies for SAMD8:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Browse Antibodies at Sigma-Aldrich
	Browse R&D Systems for Antibodies
	Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
	Browse Abnova for Monoclonal and Polyclonal Antibodies
	Search Novus for antibodies for SAMD8

Assays for SAMD8:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

Graphical View of Domain Structure for InterPro Entry Q96LT4

ProtoNet protein and cluster: Q96LT4

1 Blocks protein family: IPB001660 Sterile alpha motif SAM

UniProtKB/Swiss-Prot: SAMD8_HUMAN, Q96LT4

Similarity: Belongs to the sphingomyelin synthase family

Similarity: Contains 1 SAM (sterile alpha motif) domain

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (SAMD8)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_144660

Applied Biosystems Silencer® siRNAs for SAMD8

Sigma-Aldrich siRNA and siRNA Panels for SAMD8

Sigma-Aldrich shRNA for SAMD8

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Invitrogen Clones for SAMD8
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clone in CMV expression vector: NM_144660
Myc/DDK tagged cDNA clone in CMV expression vector: NM_144660
untagged cDNA clone in CMV expression vector: NM_144660

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_144660

1 Gene Ontology (GO) molecular function term (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0003674	molecular_function	ND	--

About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

3 Gene Ontology (GO) biological process terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0006629	lipid metabolic process	IEA	--
GO:0006665	sphingolipid metabolic process	IEA	--
GO:0006686	sphingomyelin biosynthetic process	NAS	14685263

About this table

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for SAMD8

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (SAMD8)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_144660

Sigma-Aldrich siRNA and siRNA Panels for SAMD8

Sigma-Aldrich shRNA for SAMD8

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_144660

REFSEQ mRNAs for SAMD8 gene:

NM_144660.1

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_144660

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_144660
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_144660
                                 untagged cDNA clone in CMV expression vector: NM_144660

Additional cDNA sequence:

AK057811.1 BC080593.1

3 DOTS entries:

DT.91657390 DT.75159217 DT.95250857

21 AceView cDNA sequences:

NM_144660 AK057811 AI341118 AW991245 BQ227867 BX280496 BE551749 BP355712

AL600808 BE890410 BX496935 BG778855 BI461349 BG571120 BX510136 BG332990

BC080593 BX384859 AW991585 BX462930 BU181071

highest scoring ESTs for SAMD8:

AA559273 AI374462 AK057811 AW991245 AW991585 BG571120 BU181071 AI341118 AW856914 BC080593

Unigene Cluster for SAMD8:

Sterile alpha motif domain containing 8

Hs.663616 [show with all ESTs]

Unigene Representative Sequence: BC080593

GeneLoc Exon Structure

2 Alternative Splicing Database (ASD) splice patterns (SP) for SAMD8

ExUns:	1a	·	1b	·	1c	^	2	^	3	^	4	^	5	^	6a	·	6b
SP1:					-
SP2:

About this scheme

ECgene alternative splicing isoforms for SAMD8

2 Ensembl transcripts including schematic representations:

ENST00000372690 ENST00000372687

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

SAMD8 expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for SAMD8

¹ / ² / ³

4 probe-sets matching SAMD8 gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
242062_at²^{, 3}	U133-B	1	0.46	1.00	--	--	BE890410	0.60	1.00	0.82	1
215597_x_at*²	U133-A	26	1.00	0.44	--	--	--	--	--	--	--
242062_at²	U133Plus2	1	0.46	1.00	--	--	--	--	--	--	--
215597_x_at*²	U133Plus2	26	1.00	0.44	--	--	--	--	--	--	--

About this table

Data from

(Publications) and GNF BioGPS
About these images
About these images

CGAP SAGE TAG: TTCTCTTTTT

SOURCE GeneReport for Unigene cluster: Hs.663616

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for SAMD8 gene from 3 species

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
mouse (Mus musculus)	Samd8⁵	14	sterile alpha motif domain containing 8	--	BQ176258
chicken (Gallus gallus)	SAMD8¹	--	sterile alpha motif domain containing 8	77.81(n) 86.87(a)	428946 XM_426501.2 XP_426501.2
sea squirt (Ciona intestinalis)	Cin.3111²	--	Ciona intestinalis transcribed sequence with weak more	74.63(n)	AV672012.1

About this table Species with no ortholog for SAMD8

ENSEMBL Gene Tree for SAMD8

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

Paralogs for SAMD8 gene

SGMS2² SGMS1²

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

10/308 NCBI SNPs in SAMD8 are shown (see all 308 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 80)

AB	SNP ID	Valid	Chr 10 pos	Sequence	Recs	AA Chg	Type	More	Recs	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--			--
	rs7893833^1,2	F,H	76606697(+)	`AGACTT/GAATGT`	1	--	ng3¹		4	EU EA WA	418
	rs2938852^1,2	A,C,F,H,O	76583884(-)	`ATCCAC/ATCCAT`	1	--	int¹		7	EU EA WA NA	558
	rs10824276^1,2	A,C,F,H	76546611(+)	`ACTTCG/ATTGCC`	1	--	int¹		4	EU EA WA	380
	rs1995571^1,2	A,C,F,H	76598074(-)	`atcttA/Ccggac`	1	--	int¹		4	EU EA WA	420
	rs2657291^1,2	A,C,F,H	76596709(-)	`ATGGAG/ACCTAG`	1	--	int¹		12	EU EA WA	1256
	rs2395198^1,2	A,C,F,H	76593126(+)	`GGACTA/GTTTTG`	1	--	int¹		4	EU EA WA	416
	rs7923492^1,2	A,C,F,H	76588704(+)	`ccaatT/Attgtt`	1	--	int¹		4	EU EA WA	412
	rs11001311^1,2	A,C,F,H	76594126(+)	`TATAGC/GATGGG`	1	--	int¹		8	EU EA WA NA	626
	rs7088974^1,2	A,C,F,H	76561102(+)	`TACATC/TTTGAT`	1	--	int¹		4	EU EA WA	416
	rs10824275^1,2	A,C,F,H	76544893(+)	`TTTATA/GTTTGT`	1	--	int¹		4	EU NA	260

About this table

HapMap Linkage Disequilibrium images for SAMD8 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 611575

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
About This Section

5 PubMed articles for SAMD8 gene:

Identification of a family of animal sphingomyelin synthases. (PubMed id 14685263)^{3, 4} Huitema K.... Holthuis J.C. (2004)
Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)^{3, 4} Ota T....Sugano S. (2004)
High-throughput mapping of a dynamic signaling network in mammalian cells. (PubMed id 15761153)³ Barrios-Rodiles M....Wrana J.L. (2005)
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)³ Strausberg R.L....Marra M.A. (2002)
Hepatitis C virus core protein interacts with 14-3-3 protein and activates the kinase Raf-1. (PubMed id )⁴ Aoki H.... Hino O. (2000)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 142891	HGNC: 26320	AceView: SAMD8	Ensembl:ENSG00000156671	euGenes: HUgn142891
ECgene: SAMD8	H-InvDB: SAMD8

(According to HUGE)
About This Section

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Licensable Technologies for SAMD8
(Available from WIS Yeda, Salk, Tufts)

About This Section

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section

Products for SAMD8:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for SAMD8-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for SAMD8		Browse antibodies at Sigma-Aldrich
	Explore super-pooled esiRNAs		Browse proteins at Sigma-Aldrich
	Lentivirus-delivered shRNAs for SAMD8		Browse small molecules at Sigma-Aldrich
	"Your Favorite Gene" Pathways		feedback

	Antibodies		Primer Pairs
	Cell Culture Products		ELISAs
	Flow Cytometry Kits		Protease Activity Assays & Reagents
	Cell Selection/Detection Kits & Reagents		ELISA/Assay Development Kits & Reagents
	Multiplex/Array Assay Kits & Reagents		ELISpot Kits & Development Modules
	Recombinant & Natural Proteins

	Recombinant Proteins (SAMD8)
	Search for Antibodies
	Search for Chimera RNAi
	Custom Service for Mouse Mab
	Custom Service for Rabbit Pab from Full-length Protein

Search for Antibodies & Assays

	Search for Recombinant Proteins
	Search for Antibodies

Search OriGene for SAMD8

Search Tocris compounds for SAMD8

Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies

Search Novus for antibodies for SAMD8

GeneCards Homepage - Last full update: 1 Jul 2009 Incremental update: 13 Oct 2009

Random Gene From GIFtS :

about GIFtS

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Developed at the Crown Human Genome Center & Weizmann Institute of Science

The GeneCards Human Gene Database: Copyright © 1996-2009, Weizmann Institute of Science. All Rights Reserved.
SAMD8 Gene at Home site.
Version 2.41.1
server5.xennexinc.com

SAMD8 Gene

protein-coding GIFtS: 44

Aliases &
Descriptions
for SAMD8

Summaries
for SAMD8

Genomic Location
for SAMD8

Proteins
for SAMD8

Protein Domains/
Families
for SAMD8

Gene Function
for SAMD8

Pathways & Interactions
for SAMD8

Drugs & Compounds
for SAMD8

Transcripts
for SAMD8

Expression
for SAMD8

Orthologs
for SAMD8

Paralogs
for SAMD8

SNPs/Variants
for SAMD8

Disorders & Mutations
for SAMD8

Medical News
for SAMD8

Publications
for SAMD8

Search for SAMD8

Genome Databases showing SAMD8

Other Databases showing SAMD8

Specialized Databases showing SAMD8

Licensable Technologies
for SAMD8

Services
for SAMD8

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

SAMD8 Gene

protein-coding GIFtS: 44

Aliases & Descriptionsfor SAMD8

Summariesfor SAMD8

Genomic Locationfor SAMD8

Proteinsfor SAMD8

Protein Domains/Familiesfor SAMD8

Gene Functionfor SAMD8

Pathways & Interactionsfor SAMD8

Drugs & Compoundsfor SAMD8

Transcriptsfor SAMD8

Expression for SAMD8

Orthologsfor SAMD8

Paralogsfor SAMD8

SNPs/Variantsfor SAMD8

Disorders & Mutationsfor SAMD8

Medical Newsfor SAMD8

Publicationsfor SAMD8

Search for SAMD8

Genome Databases showing SAMD8

Other Databases showing SAMD8

Specialized Databases showing SAMD8

Licensable Technologiesfor SAMD8

Servicesfor SAMD8

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for SAMD8

Summaries
for SAMD8

Genomic Location
for SAMD8

Proteins
for SAMD8

Protein Domains/
Families
for SAMD8

Gene Function
for SAMD8

Pathways & Interactions
for SAMD8

Drugs & Compounds
for SAMD8

Transcripts
for SAMD8

Expression
for SAMD8

Orthologs
for SAMD8

Paralogs
for SAMD8

SNPs/Variants
for SAMD8

Disorders & Mutations
for SAMD8

Medical News
for SAMD8

Publications
for SAMD8

Licensable Technologies
for SAMD8

Services
for SAMD8