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SAMD4B Gene

protein-coding GIFtS: 46

GC19P044525

sterile alpha motif domain containing 4B
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
About This Section

Aliases
FLJ10211 ^¹, ²
MGC99832 ^¹, ²
SMGB ^¹, ²

Descriptions
smaug homolog B ^²
sterile alpha motif domain containing 4B ^²

External Ids
HGNC: 25492¹
Entrez Gene: 55095²
UniProtKB: Q5PRF9³
Ensembl: ENSG00000179134⁷

Search outside databases for aliases for SAMD4B gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:

UCSC Golden Path with GeneCards custom track

Transcription factor binding sites upstream to the SAMD4B gene

Entrez Gene cytogenetic band: 19q13.2 Ensembl cytogenetic band: 19q13.2 HGNC cytogenetic band: 19q13.2

SAMD4B Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 19 GeneLoc Exon Structure

GeneLoc location for GC19P044525: (about GC identifiers)

Start:	44,524,948 bp from pter
End:	44,567,382 bp from pter
Size:	42,435 bases
Orientation:	plus strand

RefSeq DNA sequence:

NC_000019.8 NT_011109.15

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: SAM4B_HUMAN, Q5PRF9 (See protein sequence)

Recommended Name: Sterile alpha motif domain-containing protein 4B

Size: 694 amino acids; 75483 Da

Secondary accessions: Q6P194

Post-translational modifications:

View phosphorylation sites using PhosphoSite²

REFSEQ proteins: NP_060498.2

ENSEMBL proteins:

ENSP00000375919 ENSP00000317224

Human Recombinant Proteins

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	Browse Abcam for Recombinant Proteins
	Browse Abnova for Human Recombinant Proteins

Browse Origene for full length recombinant human proteins expressed in human HEK293 cells

Antibodies for SAMD4B:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Sigma-Aldrich Antibodies for SAMD4B
	Browse R&D Systems for Antibodies
	Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
	Browse Abnova for Monoclonal and Polyclonal Antibodies
	Search Novus for antibodies for SAMD4B

Assays for SAMD4B:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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Graphical View of Domain Structure for InterPro Entry Q5PRF9

ProtoNet protein and cluster: Q5PRF9

1 Blocks protein family: IPB001660 Sterile alpha motif SAM

UniProtKB/Swiss-Prot: SAM4B_HUMAN, Q5PRF9

Similarity: Contains 1 SAM (sterile alpha motif) domain

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (SAMD4B)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): XM_290820

Applied Biosystems Silencer® siRNAs for SAMD4B

Sigma-Aldrich siRNA for SAMD4B

Sigma-Aldrich shRNA for SAMD4B

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Browse Clone Ranger at Invitrogen for clones
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clone in CMV expression vector: NM_018028
Myc/DDK tagged cDNA clone in CMV expression vector: NM_018028
untagged cDNA clones in CMV expression vector (see all 2): NM_018028

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_018028

1 Gene Ontology (GO) molecular function term (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005515	protein binding	IPI	17353931

About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

4 Interacting proteins for SAMD4B (Q5PRF9^{1, 2}) via UniProtKB, MINT, and/or STRING
Interactant Interaction Details
GeneCard External ID(s)
YWHAZ P63104¹^,² EBI-1047489, EBI-347088 MINT-3307403 EBI-1047489, EBI-347088 MINT-3307403
YWHAB P31946¹ EBI-1047489, EBI-359815
YWHAG P61981¹ EBI-1047489, EBI-359832
YWHAQ P27348¹ EBI-1047489, EBI-359854
YWHAZ P63104¹^,² EBI-1047489, EBI-347088 MINT-3307403 EBI-1047489, EBI-347088 MINT-3307403
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(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
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Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for SAMD4B

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (SAMD4B)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): XM_290820

Sigma-Aldrich siRNA for SAMD4B

Sigma-Aldrich shRNA for SAMD4B

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_018028

REFSEQ mRNAs for SAMD4B gene:

NM_018028.2

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_018028

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_018028
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_018028
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_018028

Additional cDNA sequence:

AK091041.1 AK091238.1 AK128884.1 BC039652.1 BC054518.1 BC065211.1 BC080641.1 EF601121.1

16 DOTS entries:

DT.95178706 DT.87046989 DT.95115442 DT.92453695 DT.205925 DT.91752851 DT.100669599 DT.40200797

DT.95267756 DT.100660671 DT.91904696 DT.92068152 DT.101974455 DT.92046537 DT.95178599 DT.95367398

24/205 AceView cDNA sequences (see all 205 ):

BM142126 BC054518 AI393557 AK091041 AW075864 AI184884 AI400728 BU185633

CK300188 BQ582213 AW029159 BC039652 BC065211 BC080641 BQ022323 CN483213

BQ423073 BQ888157 AI830476 AI953664 AW075625 AK091238 AA149530 BI963084

highest scoring ESTs for SAMD4B:

AA024814 AA024920 AA149818 AA248049 AA355931 AA527686 AA939245 AI143527 AI160336 AI263762

Unigene Cluster for SAMD4B:

Sterile alpha motif domain containing 4B

Hs.612332 [show with all ESTs]

Unigene Representative Sequence: BC054518

GeneLoc Exon Structure

4 Alternative Splicing Database (ASD) splice patterns (SP) for SAMD4B

ExUns:	1	^	2	^	3	^	4	^	5	^	6	^	7	^	8	^	9	^	10	^	11a	·	11b	^	12	^	13	^	14a	·	14b
SP1:																							-
SP2:																							-				-
SP3:
SP4:																							-

About this scheme

ECgene alternative splicing isoforms for SAMD4B

2 Ensembl transcripts including schematic representations:

ENST00000392067 ENST00000314471

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

SAMD4B expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for SAMD4B

¹ / ² / ³

8 probe-sets matching SAMD4B gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
90083_at²^{, 3}	U95-E	1	1.00	1.00	1.00	1.00	AA149818	0.80	1.00	0.91	1
47902_at²^{, 3}	U95-B	1	0.62	1.00	-0.54	0.20	W74622	0.60	1.00	0.82	1
226714_at²^{, 3}	U133-B	1	1.00	1.00	--	--	AA149818	0.80	1.00	0.91	1
220457_at²^{, 3}	U133-A	1	1.00	1.00	--	--	NM_018028	0.60	1.00	0.82	1
229871_at²^{, 3}	U133-B	1	0.82	1.00	--	--	W74622	0.60	1.00	0.82	1
226714_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--
220457_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--
229871_at²	U133Plus2	1	0.82	1.00	--	--	--	--	--	--	--

GeneDecks SAMD4B for binary patterns associated with the probe-sets selected above

About GeneDecksing

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Data from

(Publications) and GNF BioGPS
About these images
About these images

CGAP SAGE TAG: ACCTCACTTA

SOURCE GeneReport for Unigene cluster: Hs.612332

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for SAMD4B gene from 5/6 species (see all 6 )

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
dog (Canis familiaris)	SAMD4B¹	--	sterile alpha motif domain containing 4B	94.72(n) 98.41(a)	484511 XM_848215.1 XP_853308.1
chimpanzee (Pan troglodytes)	SAMD4B¹	--	sterile alpha motif domain containing 4B	98.46(n) 98.85(a)	456020 XM_001136382.1 XP_001136382.1
rat (Rattus norvegicus)	Samd4b¹	--	sterile alpha motif domain containing 4B	92.87(n) 97.82(a)	308473 XM_218384.3 XP_218384.1
mouse (Mus musculus)	Samd4b^{1, 5}	7⁵	sterile alpha motif domain containing 4B^{1, 5}	92.92(n)¹ 97.67(a)¹	233033¹ NM_175021.2¹ NP_778186.1¹ AK133691⁵ AK185673⁵ (see all 7)
zebrafish (Danio rerio)	57088700²	--	--	81.59(n)	57088700

About this table Species with no ortholog for SAMD4B

ENSEMBL Gene Tree for SAMD4B

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

Paralogs for SAMD4B gene

SAMD4A²

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

10/201 NCBI SNPs in SAMD4B are shown (see all 201 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 57)

AB	SNP ID	Valid	Chr 19 pos	Sequence	Recs	AA Chg	Type	More	Recs	Allele freq	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--				--
	rs36270^1,2	C,F,H,O	44566785(+)	`GACCCC/TCAAGC`	1	--	ut3¹		13		EA NA EU WA	932
	rs30460^1,2	C,F,H	44524353(-)	`CCGATG/TGGAAT`	1	--	ng5¹		4		MN EA WA	476
--	rs10412658^1,2	C	44567391(+)	`AAAAAA/CCAAAA`	1	--	ng3¹		0	--	--	--	--
	rs562638^1,2	A,C,F,H,O	44541629(+)	`TTGTGA/GTATGA`	1	--	int¹		6		EU EA WA NA	598
	rs11666780^1,2	A,C,F,H	44545367(+)	`GGCAAT/CGGCAA`	1	--	int¹		7		EU EA WA NA	690
	rs30454^1,2	A,C,F,H	44536016(-)	`CTACAC/TAGTAA`	1	--	int¹		7		MN EU EA WA NA	786
	rs30455^1,2	C,F,H,O	44535463(-)	`TCCCTC/TGGGAG`	1	--	int¹		5		NA EU EA WA	432
	rs917319^1,2	A,C,F,H	44548458(+)	`ggcaaG/Atcatt`	1	--	int¹		3		EA WA	290
	rs1368442^1,2	A,C,F,H	44527009(-)	`GGAATC/ATGAGG`	1	--	int¹		8		EU EA WA	840
--	rs30457^1,2	A,C,F,H	44532389(-)	`TGCAAG/CAAAgc`	1	--	int¹		3		EU EA	300

About this table

HapMap Linkage Disequilibrium images for SAMD4B (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
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9 PubMed articles for SAMD4B gene:

A probability-based approach for high-throughput protein phosphorylation analysis and site localization. (PubMed id 16964243)^{3, 4} Beausoleil S.A.... Gygi S.P. (2006)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{3, 4} Gerhard D.S....Malek J. (2004)
Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis. (PubMed id 18220336)⁴ Cantin G.T....Yates J.R. III (2008)
A quantitative atlas of mitotic phosphorylation. (PubMed id 18669648)⁴ Dephoure N.... Gygi S.P. (2008)
Large-scale mapping of human protein-protein interactions by mass spectrometry. (PubMed id 17353931)³ Ewing R.M....Figeys D. (2007)
Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)³ Ota T....Sugano S. (2004)
Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization. (PubMed id 15324660)³ Jin J....Pawson T. (2004)
Large-scale characterization of HeLa cell nuclear phosphoproteins. (PubMed id 15302935)³ Beausoleil S.A....Gygi S.P. (2004)
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)³ Strausberg R.L....Marra M.A. (2002)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
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Entrez Gene: 55095	HGNC: 25492	AceView: FLJ10211	Ensembl:ENSG00000179134	euGenes: HUgn55095
ECgene: SAMD4B	H-InvDB: SAMD4B

(According to HUGE)
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(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
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Licensable Technologies for SAMD4B
(Available from WIS Yeda, Salk, Tufts)

About This Section

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
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Products for SAMD4B:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for SAMD4B-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for SAMD4B		Antibodies for SAMD4B
	Explore super-pooled esiRNAs		Browse proteins at Sigma-Aldrich
	Lentivirus-delivered shRNAs for SAMD4B		Browse small molecules at Sigma-Aldrich
	"Your Favorite Gene" Pathways		feedback

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	Multiplex/Array Assay Kits & Reagents		ELISpot Kits & Development Modules
	Recombinant & Natural Proteins

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SAMD4B Gene

protein-coding GIFtS: 46

Aliases &
Descriptions
for SAMD4B

Summaries
for SAMD4B

Genomic Location
for SAMD4B

Proteins
for SAMD4B

Protein Domains/
Families
for SAMD4B

Gene Function
for SAMD4B

Pathways & Interactions
for SAMD4B

Drugs & Compounds
for SAMD4B

Transcripts
for SAMD4B

Expression
for SAMD4B

Orthologs
for SAMD4B

Paralogs
for SAMD4B

SNPs/Variants
for SAMD4B

Disorders & Mutations
for SAMD4B

Medical News
for SAMD4B

Publications
for SAMD4B

Search for SAMD4B

Genome Databases showing SAMD4B

Other Databases showing SAMD4B

Specialized Databases showing SAMD4B

Licensable Technologies
for SAMD4B

Services
for SAMD4B

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

SAMD4B Gene

protein-coding GIFtS: 46

Aliases & Descriptionsfor SAMD4B

Summariesfor SAMD4B

Genomic Locationfor SAMD4B

Proteinsfor SAMD4B

Protein Domains/Familiesfor SAMD4B

Gene Functionfor SAMD4B

Pathways & Interactionsfor SAMD4B

Drugs & Compoundsfor SAMD4B

Transcriptsfor SAMD4B

Expression for SAMD4B

Orthologsfor SAMD4B

Paralogsfor SAMD4B

SNPs/Variantsfor SAMD4B

Disorders & Mutationsfor SAMD4B

Medical Newsfor SAMD4B

Publicationsfor SAMD4B

Search for SAMD4B

Genome Databases showing SAMD4B

Other Databases showing SAMD4B

Specialized Databases showing SAMD4B

Licensable Technologiesfor SAMD4B

Servicesfor SAMD4B

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for SAMD4B

Summaries
for SAMD4B

Genomic Location
for SAMD4B

Proteins
for SAMD4B

Protein Domains/
Families
for SAMD4B

Gene Function
for SAMD4B

Pathways & Interactions
for SAMD4B

Drugs & Compounds
for SAMD4B

Transcripts
for SAMD4B

Expression
for SAMD4B

Orthologs
for SAMD4B

Paralogs
for SAMD4B

SNPs/Variants
for SAMD4B

Disorders & Mutations
for SAMD4B

Medical News
for SAMD4B

Publications
for SAMD4B

Licensable Technologies
for SAMD4B

Services
for SAMD4B