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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

RXFP4 Gene

protein-coding   GIFtS: 48
GCID: GC01P155911

relaxin/insulin-like family peptide receptor 4

(Previous names: G protein-coupled receptor 100, relaxin 3 receptor 2, relaxin...)
(Previous symbols: GPR100, RLN3R2)
 Explore 4 diseases affiliated with
RXFP4 via our new
 Human Malady Compendium 
Biological research products
for RXFP4
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Relaxin/Insulin-Like Family Peptide Receptor 41 2     Relaxin 3 Receptor 21 2
Relaxin Family Peptide Receptor 41 2 3     Insulin-Like Peptide INSL5 Receptor2 3
GPR1001 2 3     G-Protein Coupled Receptor 1002 3
RLN3R21 2 3     G-Protein Coupled Receptor GPCR1422 3
GPCR1421 2     RLN3 Receptor 22 3
RXFPR41 2     Relaxin-3 Receptor 22
G Protein-Coupled Receptor 1001 2     

External Ids:    HGNC: 146661   Entrez Gene: 3394032   Ensembl: ENSG000001730807   OMIM: 6090435   UniProtKB: Q8TDU93   

Export aliases for RXFP4 gene to outside databases

Previous GC identifers: GC01P154178 GC01P127272


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for RXFP4:
GPR100 is a member of the rhodopsin family of G protein-coupled receptors (GPRs) (Fredriksson et al., 2003 (PubMed
14623098)).(supplied by OMIM, Mar 2008)

UniProtKB/Swiss-Prot: RL3R2_HUMAN, Q8TDU9
Function: High affinity receptor for INSL5. Also acts as receptor for RLN3/relaxin-3, as well as bradykinin and
kallidin. Binding of the ligand inhibit cAMP accumulation

Gene Wiki entry for RXFP4 (Relaxin/insulin-like family peptide receptor 4)


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000001.10  NC_018912.1  NT_004487.19  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the RXFP4 gene promoter:
         NF-1/L   NF-1   AML1a   POU3F1   AP-4   C/EBPalpha   CREB   deltaCREB   c-Myb   ATF6   
         Other transcription factors

Browse SwitchGear Promoter luciferase reporter plasmids
   Search SABiosciences Chromatin IP Primers for RXFP4

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat RXFP4


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 1q22   Ensembl cytogenetic band:  1q22   HGNC cytogenetic band: 1q22

RXFP4 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
RXFP4 gene location

GeneLoc information about chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01P155911:  view genomic region     (about GC identifiers)

Start:
155,911,480 bp from pter      End:
155,912,625 bp from pter
Size:
1,146 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: RL3R2_HUMAN, Q8TDU9 (See protein sequence)
Recommended Name: Relaxin-3 receptor 2  
Size: 374 amino acids; 41141 Da
Subcellular location: Cell membrane; Multi-pass membrane protein
Sequence caution: Sequence=BAC05844.1; Type=Erroneous gene model prediction;
Secondary accessions: B0M0L4 Q3MJB1 Q8NGZ8

Explore the universe of human proteins at neXtProt for RXFP4: NX_Q8TDU9

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q8TDU9

  • RXFP4 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_871001.1  
    ENSEMBL proteins: 
     ENSP00000357301  
    Reactome Protein details: Q8TDU9
    Human Recombinant Protein Products: 
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    Uscn Proteins for RXFP4

    Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005886plasma membrane TAS--
    GO:0016021integral to membrane IEA--


    RXFP4 for ontologies           About GeneDecksing



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    Uscn ELISAs and CLIAs for RXFP4


    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    RXFP4 for domains           About GeneDecksing

    3 InterPro domains/families:
     IPR017452 GPCR_Rhodpsn_7TM
     IPR000276 GPCR_Rhodpsn
     IPR000248 ATII_rcpt

    Graphical View of Domain Structure for InterPro Entry Q8TDU9

    ProtoNet protein and cluster: Q8TDU9

    UniProtKB/Swiss-Prot: RL3R2_HUMAN, Q8TDU9
    Similarity: Belongs to the G-protein coupled receptor 1 family


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: RL3R2_HUMAN, Q8TDU9
    Function: High affinity receptor for INSL5. Also acts as receptor for RLN3/relaxin-3, as well as bradykinin and
    kallidin. Binding of the ligand inhibit cAMP accumulation

    miRNA
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    Browse MicroRNA Expression Plasmids
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat RXFP4
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    Inhib. RNA
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    OriGene shRNA RFP: RXFP4
    OriGene siRNA: RXFP4
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    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RXFP4 

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    In Situ Assay
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RXFP4

    Gene Ontology (GO): 1 molecular function term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004945angiotensin type II receptor activity IEA--


    RXFP4 for ontologies           About GeneDecksing


    2 GenomeRNAi human phenotypes for RXFP4:
     High actin ratio cells  Increased circadian period len 

    Animal Models:
         Mouse knock-out Rxfp4tm1Lex for RXFP4
         1 MGI phenotypic allele for Rxfp4 (no phenotypes)

    RXFP4 for phenotypes           About GeneDecksing


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways  About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1Class A/1 (Rhodopsin-like receptors)
    Class A/1 (Rhodopsin-like receptors)1.00
    Peptide ligand-binding receptors0.61
    GPCR ligand binding0.75
    G alpha (i) signalling events0.44
    2Signaling by GPCR
    Signaling by GPCR1.00
    Signal Transduction0.56
    GPCR downstream signaling0.89
    3Relaxin receptors
    Relaxin receptors1.00

    Pathway sources
    See GeneCards unified pathways
    Show all pathways


    5/8        Reactome Pathways for RXFP4 (see all 8)
        GPCR downstream signaling
    Peptide ligand-binding receptors
    Signal Transduction
    Signaling by GPCR
    G alpha (i) signalling events



    RXFP4 for pathways           About GeneDecksing

    Interactions:

        SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for RXFP4

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    5/145 Interacting proteins for RXFP4 (Q8TDU93 ENSP000003573014) via UniProtKB, MINT, STRING, and/or I2D (see all 145)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    KNG1P010423, ENSP000002650234I2D: score=1 STRING: ENSP00000265023
    RLN3Q8WXF33I2D: score=2 
    ADCY2ENSP000003429524STRING: ENSP00000342952
    ADCY5ENSP000004193614STRING: ENSP00000419361
    ADCY9ENSP000002940164STRING: ENSP00000294016
    About this table

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section

    RXFP4 for compounds           About GeneDecksing

    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for RXFP4

    2 HMDB Compounds for RXFP4    About this table
    CompoundSynonyms CAS #PubMed Ids
    BradykininCallidin I (see all 5)58-82-2--
    Cyclic AMPCyclic AMP (see all 19)60-92-4--
    2 Novoseek chemical compound relationships for RXFP4 gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    adenylate 23.4 2 14522967 (1), 17293890 (1)
    calcium 0 3 14522967 (1), 14530218 (1)

    Search CenterWatch for drugs/clinical trials and news about RXFP4 / RL3R2 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for RXFP4 gene: 
    NM_181885.2  

    Unigene Cluster for RXFP4:

    Relaxin/insulin-like family peptide receptor 4
    Hs.449914  [show with all ESTs]
    Unigene Representative Sequence: BC101509
    1 Ensembl transcript including schematic representation, and UCSC links where relevant:
    ENST00000368318(uc010pgs.2)

    miRNA
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    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat RXFP4
    Search QIAGEN for miScript miRNA Assays for microRNAs that regulate RXFP4
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    Inhib. RNA
    Products:
         
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    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for RXFP4 (see all 4)
    OriGene shRNA RFP: RXFP4
    OriGene siRNA: RXFP4
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    Clone
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    Primer
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      QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat RXFP4
      QIAGEN QuantiFast Probe-based Assays in human, mouse, rat RXFP4

    Additional cDNA sequence: 

    AY170824.1 AY288415.1 AY394502.1 BC101507.1 BC101509.1 EU432129.1 

    1 DOTS entry:

    DT.100693885 

    5 AceView cDNA sequences:

    NM_181885 AY288415 AY170824 AY394502 BU101743 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    RXFP4 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: --

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See RXFP4 Protein Expression from SPIRE MOPED and PaxDB
    SOURCE GeneReport for Unigene cluster: Hs.449914

    UniProtKB/Swiss-Prot: RL3R2_HUMAN, Q8TDU9
    Tissue specificity: Expressed in a broader range of tissues including brain, kidney, testis, thymus, placenta,
    prostate, salivary gland, thyroid and colon

        SABiosciences Expression via Pathway-Focused PCR Array including RXFP4: 
              G Protein Coupled Receptors 384HT in human mouse rat

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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RXFP4

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section
      --

    ENSEMBL Gene Tree for RXFP4 (if available)
    TreeFam Gene Tree for RXFP4 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for RXFP4 gene
    RXFP32  BDKRB12  AGTR22  AGTR12  GPR252  CXCR72  APLNR2  GPR152  
    BDKRB22  GPR1822  
    5 SIMAP similar genes for RXFP4 using alignment to 1 protein entry:     RL3R2_HUMAN:
    RXFP3    AGTR1    NPBWR1    NPBWR2    AGTR2

    RXFP4 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/86 NCBI SNPs in RXFP4 are shown (see all 86    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 1 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1995369361,2
    --155909560(+) AAAAG-/AAAAAA 1 -- us2k10--------
    rs66596491,2
    C--155909569(+) AAAAAA/TTTTTT 1 -- us2k1 trp30--------
    rs113006751,2
    C,F--155909569(+) AAAAAT/-TTTTT 1 -- us2k13Minor allele frequency- -:0.50NA CSA 6
    rs1873633821,2
    --155909843(+) TACAGA/GCATGA 1 -- us2k10--------
    rs1494007711,2
    --155909904(+) ATTCTA/GGGTGG 1 -- us2k10--------
    rs610272341,2
    C,--155910069(+) CAACAT/CGTTGG 1 -- us2k12Minor allele frequency- C:0.04WA 120
    rs1162179581,2
    --155910411(+) GATGAG/TGTGTG 1 -- us2k11Minor allele frequency- T:0.01NA 120
    rs1917825641,2
    --155910473(+) AGTAGA/GGGGAC 1 -- us2k10--------
    rs1474986331,2
    --155910479(+) GGGACC/TGCTGA 1 -- us2k10--------
    rs1115850291,2
    --155910523(+) AAAGGC/TGGCGA 1 -- us2k12Minor allele frequency- T:0.04CSA WA 119

    HapMap Linkage Disequilibrium report for RXFP4 (155911480 - 155912625 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 1 variation for RXFP4
         1 CNV: 6789

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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    RXFP4 for disorders           About GeneDecksing

    OMIM gene information: 609043    OMIM disorders: --

    4 diseases for RXFP4:    About MalaCards
    cryptorchidism    hermaphroditism    thyroiditis    prostatitis


    Export disorders for RXFP4 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for RXFP4 gene, integrated from 9 sources (see all 29):
    (articles sorted by number of sources associating them with RXFP4)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. INSL5 is a high affinity specific agonist for GPCR142 (GPR100). (PubMed id 15525639)1, 2, 9 Liu C....Lovenberg T.W. (2005)
    2. Identification of relaxin-3/INSL7 as a ligand for GPCR142. (PubMed id 14522967)1, 2, 9 Liu C....Lovenberg T.W. (2003)
    3. Identification and characterisation of GPR100 as a novel human G- protein-coupled bradykinin receptor. (PubMed id 14530218)1, 2, 9 Boels K. and Schaller H.C. (2003)
    4. Receptors for relaxin family peptides. (PubMed id 15956688)1, 3, 9 Bathgate R.A....Summers R.J. (2005)
    5. The DNA sequence and biological annotation of human chromosome 1. (PubMed id 16710414)1, 2 Gregory S.G.... Bentley D.R. (2006)
    6. International Union of Pharmacology LVII: recommendations for the nomenclature of receptors for relaxin family peptides. (PubMed id 16507880)1, 3 Bathgate R.A....Summers R.J. (2006)
    7. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    8. Seven evolutionarily conserved human rhodopsin G protein-coupled receptors lacking close relatives. (PubMed id 14623098)1, 2 Fredriksson R....Schioeth H.B. (2003)
    9. Identification of G protein-coupled receptor genes from the human genome sequence. (PubMed id 12044878)1, 2 Takeda S.... Mitaku S. (2002)
    10. Identification of the domains in RXFP4 (GPCR142) responsible for the high affinity binding and agonistic activity of INSL5 at RXFP4 compared to RXFP3 (GPCR135). (PubMed id 18582868)1, 9 Zhu J....Liu C. (2008)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 339403 HGNC: 14666 AceView: GPR100 Ensembl:ENSG00000173080 euGenes: HUgn339403
    ECgene: RXFP4 H-InvDB: RXFP4

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for RXFP4 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for RXFP4 gene:
    Search GeneIP for patents involving RXFP4

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     Browse OriGene Antibodies   OriGene shRNA RFP for RXFP4  
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     OriGene Custom Antibody Services for RXFP4   OriGene Custom Protein Services for RXFP4  
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     QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat RXFP4
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     Regulatory tfbs in RXFP4 promoter
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     RXFP4 Proteins, Antibodies, CLIAs, and ELISAs
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     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RXFP4
           
    GeneCards Homepage - Last full update: 18 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 27 Apr 2013

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