RXFP4 Gene
protein-coding GIFtS: 48
GCID: GC01P155911
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relaxin/insulin-like family peptide receptor 4(Previous names: G protein-coupled receptor 100, relaxin 3 receptor 2, relaxin...) (Previous symbols: GPR100, RLN3R2)
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Aliases for RXFP4 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb) About This Section
|
| Aliases |
|---|
| Relaxin/Insulin-Like Family Peptide Receptor 41 2 | | Relaxin 3 Receptor 21 2 | | Relaxin Family Peptide Receptor 41 2 3 | | Insulin-Like Peptide INSL5 Receptor2 3 | | GPR1001 2 3 | | G-Protein Coupled Receptor 1002 3 | | RLN3R21 2 3 | | G-Protein Coupled Receptor GPCR1422 3 | | GPCR1421 2 | | RLN3 Receptor 22 3 | | RXFPR41 2 | | Relaxin-3 Receptor 22 | | G Protein-Coupled Receptor 1001 2 | | |
Export aliases for RXFP4 gene to outside databasesPrevious GC identifers: GC01P154178 GC01P127272 |
Summaries for RXFP4 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
| Entrez Gene summary for RXFP4: GPR100 is a member of the rhodopsin family of G protein-coupled receptors (GPRs) (Fredriksson et al., 2003 (PubMed14623098)).(supplied by OMIM, Mar 2008) UniProtKB/Swiss-Prot: RL3R2_HUMAN, Q8TDU9Function: High affinity receptor for INSL5. Also acts as receptor for RLN3/relaxin-3, as well as bradykinin andkallidin. Binding of the ligand inhibit cAMP accumulation Gene Wiki entry for RXFP4 (Relaxin/insulin-like family peptide receptor 4)
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Genomic Views for RXFP4 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics) About This Section
| RefSeq DNA sequence:- NC_000001.10 NC_018912.1 NT_004487.19
Regulatory elements: SABiosciences Regulatory transcription factor binding sites in the RXFP4 gene promoter: NF-1/L NF-1 AML1a POU3F1 AP-4 C/EBPalpha CREB deltaCREB c-Myb ATF6 Other transcription factors
Search SABiosciences Chromatin IP Primers for RXFP4
Epigenetics:
|  | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat RXFP4 |
Genomic Location: Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 1q22 Ensembl cytogenetic band: 1q22 HGNC cytogenetic band: 1q22RXFP4 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different)

GeneLoc information about chromosome 1 GeneLoc Exon Structure GeneLoc location for GC01P155911: view genomic region
(about GC identifiers)
Start:
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155,911,480 bp from pter |
End:
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155,912,625 bp from pter |
Size:
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1,146 bases |
Orientation:
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plus strand |
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Proteins for RXFP4 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
| UniProtKB/Swiss-Prot: RL3R2_HUMAN, Q8TDU9 (See
protein sequence)Recommended Name: Relaxin-3 receptor 2 Size: 374 amino acids; 41141 Da
Subcellular location: Cell membrane; Multi-pass membrane protein
Sequence caution: Sequence=BAC05844.1; Type=Erroneous gene model prediction;
Secondary accessions: B0M0L4 Q3MJB1 Q8NGZ8Explore the universe of human proteins at neXtProt for RXFP4: NX_Q8TDU9
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q8TDU9 RXFP4 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_871001.1 ENSEMBL proteins: ENSP00000357301 Reactome Protein details: Q8TDU9 Human Recombinant Protein Products:
Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0005886 | plasma membrane |
TAS | -- | | GO:0016021 | integral to membrane |
IEA | -- |
RXFP4 for ontologies About GeneDecksing
RXFP4 Antibody Products: Assay Products for RXFP4: |
Protein
Domains / Families for RXFP4 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
RXFP4 for domains About GeneDecksing
3 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry Q8TDU9ProtoNet protein and cluster: Q8TDU9 UniProtKB/Swiss-Prot: RL3R2_HUMAN, Q8TDU9Similarity: Belongs to the G-protein coupled receptor 1 family |
Function for RXFP4 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
| Function Summary: UniProtKB/Swiss-Prot: RL3R2_HUMAN, Q8TDU9Function: High affinity receptor for INSL5. Also acts as receptor for RLN3/relaxin-3, as well as bradykinin andkallidin. Binding of the ligand inhibit cAMP accumulation
Clone Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for RXFP4 (see all 2) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for RXFP4 OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector: RXFP4 (NM_181885) | |  | Browse Sino Biological Human cDNA Clones | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for RXFP4 | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RXFP4  |
In Situ Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RXFP4 |
Gene Ontology (GO): 1 molecular function term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0004945 | angiotensin type II receptor activity |
IEA | -- |
RXFP4 for ontologies About GeneDecksing
2 GenomeRNAi human phenotypes for RXFP4: Animal Models: Mouse knock-out Rxfp4tm1Lex for RXFP4 1 MGI phenotypic allele for Rxfp4 (no phenotypes)
RXFP4 for phenotypes About GeneDecksing
|
Pathways & Interactions for RXFP4 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
| Unified GeneCards pathways  About this table  See pathways by source
| Super-pathway | contained gene-specific pathways |
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| 1 | Class A/1 (Rhodopsin-like receptors) | | | 2 | Signaling by GPCR | | | 3 | Relaxin receptors | |
Pathway sources See GeneCards unified pathways Show all pathways
5/8
Reactome Pathways for RXFP4 (see all 8)
RXFP4 for pathways About GeneDecksing
Interactions:
SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for RXFP4
STRING Interaction
Network Preview (showing 5 interactants - click image to see 25)
 5/145 Interacting proteins for RXFP4 (Q8TDU93 ENSP000003573014) via UniProtKB, MINT, STRING, and/or I2D (see all 145)About this table
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Drugs & Compounds for RXFP4 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
RXFP4 for compounds About GeneDecksing
Browse Tocris compounds for RXFP4
2 HMDB Compounds for RXFP4 About this table 2 Novoseek chemical compound relationships for RXFP4 gene About this table
| Compound |
-log (P-Val) |
Hits |
PubMed IDs for Articles with Shared Sentences (# sentences) |
| adenylate |
23.4 |
2 |
14522967 (1), 17293890 (1) |
| calcium |
0 |
3 |
14522967 (1), 14530218 (1) |
Search CenterWatch for drugs/clinical trials and news about RXFP4 / RL3R2 
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Transcripts for RXFP4 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank, RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
) About This Section
| REFSEQ mRNAs for RXFP4 gene: NM_181885.2 Unigene Cluster for RXFP4: Relaxin/insulin-like family peptide receptor 4 Hs.449914 [show with all ESTs]Unigene Representative Sequence: BC1015091 Ensembl transcript including schematic representation, and UCSC links where relevant: ENST00000368318(uc010pgs.2)
Clone Products: |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for RXFP4 (see all 2) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for RXFP4 OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector: RXFP4 (NM_181885) | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for RXFP4 | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RXFP4  |
Additional cDNA sequence: AY170824.1 AY288415.1 AY394502.1 BC101507.1 BC101509.1 EU432129.1 1 DOTS entry: DT.100693885 5 AceView cDNA sequences: NM_181885 AY288415 AY170824 AY394502 BU101743 GeneLoc Exon Structure
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Expression for RXFP4 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| RXFP4 expression in normal human tissues (normalized intensities) See probesets specificity/sensitivity at GeneAnnot About this imageBioGPS CGAP TAG: --
About this image See RXFP4 Protein Expression from SPIRE MOPED and PaxDB SOURCE GeneReport for Unigene cluster: Hs.449914 UniProtKB/Swiss-Prot: RL3R2_HUMAN, Q8TDU9Tissue specificity: Expressed in a broader range of tissues including brain, kidney, testis, thymus, placenta,prostate, salivary gland, thyroid and colon SABiosciences Expression via Pathway-Focused PCR Array including RXFP4: | G Protein Coupled Receptors 384HT in human mouse rat |
Primer Products: |  | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for RXFP4 Browse OriGene validated miRNA SYBR primer pairs
| |  | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat RXFP4 | |  | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat RXFP4 | |  | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat RXFP4 | In Situ Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RXFP4 |
Orthologs for RXFP4 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
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--
ENSEMBL Gene Tree for RXFP4 (if available) TreeFam Gene Tree for RXFP4 (if available)  |
Paralogs for RXFP4 gene(Paralogs according to
1HomoloGene, 2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68) About This Section
| Paralogs for RXFP4 gene
- RXFP32 BDKRB12 AGTR22 AGTR12 GPR252 CXCR72 APLNR2 GPR152
- BDKRB22 GPR1822
5 SIMAP similar genes for RXFP4 using alignment to 1 protein entry: RL3R2_HUMAN:RXFP3 AGTR1 NPBWR1 NPBWR2 AGTR2
RXFP4 for paralogs About GeneDecksing
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Genomic Variants for RXFP4 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical significance | Chr 1 pos | Sequence | # | AA Chg | Type | More | # | Allele freq | Pop | Total sample | More |
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HapMap Linkage Disequilibrium report for RXFP4 (155911480 - 155912625 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions) Database of Genomic Variants (DGV): 1 variation for RXFP4 1 CNV: 6789  | SABiosciences Cancer Mutation PCR Assays |
|  | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing RXFP4 |
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Disorders
/ Diseases for RXFP4 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
RXFP4 for disorders About GeneDecksing
OMIM gene information: 609043
OMIM disorders: --
4 diseases for RXFP4: About MalaCardscryptorchidism hermaphroditism thyroiditis prostatitis Export disorders for RXFP4 gene to outside databases
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Publications for RXFP4 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for RXFP4 gene, integrated from 9 sources (see all 29): (articles sorted by number of sources associating them with RXFP4) | |  | Utopia: connect your pdf to the dynamic world of online information |
- INSL5 is a high affinity specific agonist for GPCR142 (GPR100). (PubMed id 15525639)1, 2, 9 Liu C....Lovenberg T.W. (2005)
- Identification of relaxin-3/INSL7 as a ligand for GPCR142. (PubMed id 14522967)1, 2, 9 Liu C....Lovenberg T.W. (2003)
- Identification and characterisation of GPR100 as a novel human G- protein-coupled bradykinin receptor. (PubMed id 14530218)1, 2, 9 Boels K. and Schaller H.C. (2003)
- Receptors for relaxin family peptides. (PubMed id 15956688)1, 3, 9 Bathgate R.A....Summers R.J. (2005)
- The DNA sequence and biological annotation of human chromosome 1. (PubMed id 16710414)1, 2 Gregory S.G.... Bentley D.R. (2006)
- International Union of Pharmacology LVII: recommendations for the nomenclature of receptors for relaxin family peptides. (PubMed id 16507880)1, 3 Bathgate R.A....Summers R.J. (2006)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- Seven evolutionarily conserved human rhodopsin G protein-coupled receptors lacking close relatives. (PubMed id 14623098)1, 2 Fredriksson R....Schioeth H.B. (2003)
- Identification of G protein-coupled receptor genes from the human genome sequence. (PubMed id 12044878)1, 2 Takeda S.... Mitaku S. (2002)
- Identification of the domains in RXFP4 (GPCR142) responsible for the high affinity binding and agonistic activity of INSL5 at RXFP4 compared to RXFP3 (GPCR135). (PubMed id 18582868)1, 9 Zhu J....Liu C. (2008)
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External Searches for RXFP4 gene
(in PubMed,
OMIM, and NCBI Bookshelf) About This Section
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Genome Databases showing RXFP4 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
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Other Databases showing RXFP4 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing RXFP4 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL, Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot) About This Section
|
| Name | Description |
| PharmGKB entry for RXFP4 | Pharmacogenomics, SNPs, Pathways |
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About This Section
| Patent Information for RXFP4 gene: Search GeneIP for patents involving RXFP4
GeneCards and IP: Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
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Products for RXFP4 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences), In Situ Hybridization Assays from Advanced Cell Diagnostics About This Section
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| | | | Browse OriGene Antibodies | | OriGene shRNA RFP for RXFP4 | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for RXFP4 | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for RXFP4 | | Browse OriGene Protein Over-expression Lysates | | Browse OriGene Fluorogenic Cell Assay Kits | | OriGene siRNA for RXFP4 | | Browse 3'-UTR reporter clones for miRNA target validation | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for RXFP4 | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for RXFP4 | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | Browse OriGene full length recombinant human proteins expressed in human HEK293 cells | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling | | OriGene Custom Antibody Services for RXFP4 | | OriGene Custom Protein Services for RXFP4 | | OriGene Custom Immunoassay Development | | |
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| | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat RXFP4 | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing RXFP4 | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat RXFP4 | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat RXFP4 | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat RXFP4 | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat RXFP4 |
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 | | RXFP4 Proteins, Antibodies, CLIAs, and ELISAs |
| | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RXFP4 |
|  |  |  | | | ThermoFisher Antibodies for RXFP4 |
| | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RXFP4 |
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