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RSPO2 Gene

protein-coding   GIFtS: 50

GC08M108981
R-spondin 2 homolog (Xenopus laevis)
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
CRISTIN2 2
MGC35555 1, 2
MGC43342 2
hRspo2 3
Descriptions
R-spondin 2 homolog (Xenopus laevis) 2
R-spondin family, member 2 2
Roof plate-specific spondin-2 3
External Ids
HGNC: 285831
Entrez Gene: 3404192
UniProtKB: Q6UXX93
Ensembl: ENSG000001476557
Search outside databases for aliases for RSPO2 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for RSPO2:
R-spondins (RSPOs), such as RSPO2, are secreted proteins that regulate beta-catenin (CTNNB1; MIM
116806) signaling (Kim et al., 2006 [PubMed 16357527]).[supplied by OMIM]

UniProtKB/Swiss-Prot: RSPO2_HUMAN, Q6UXX9
Function: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene
activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway, possibly via a direct
interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor
signaling pathway that may not use frizzled/LRP receptors. Probably also acts as a ligand for
frizzled and LRP receptors (By similarity)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the RSPO2 gene  

Entrez Gene cytogenetic band: 8q23.1   Ensembl cytogenetic band:  8q23.1   HGNC cytogenetic band: 8q23.1

RSPO2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 8         GeneLoc Exon Structure

GeneLoc location for GC08M108981:     (about GC identifiers)

Start:
108,980,721 bp from pter
End:
109,165,052 bp from pter
Size:
184,332 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000008.9  NT_008046.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: RSPO2_HUMAN, Q6UXX9 (See protein sequence)
Recommended Name: R-spondin-2 precursor  
Size: 243 amino acids; 28315 Da
Subunit: Interacts with WNT1. Binds heparin (By similarity)
Subcellular location: Secreted (By similarity)
Miscellaneous: Upon injection into mice, it induces rapid onset of crypt cell proliferation
involving beta-catenin stabilization. It also displays efficacy in a model of chemotherapy-induced
intestinal mucositis
Secondary accessions: Q4G0U4 Q8N6X6
Alternative splicing: 3 isoforms:  Q6UXX9-1   Q6UXX9-2   Q6UXX9-3   

REFSEQ proteins: NP_848660.3  

ENSEMBL proteins: 
ENSP00000276659 ENSP00000367698 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
R&D Systems Recombinant & Natural Proteins for RSPO2 (R-Spondin 2)
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (RSPO2)
Human Recombinant Proteins from Abnova (RSPO2)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

1 Gene Ontology (GO) cellular component term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005576 extracellular region IEA--
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Antibodies for RSPO2: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Browse Antibodies at Sigma-Aldrich
R&D Systems Antibodies for RSPO2 (R-Spondin 2)
Antibodies from Abcam (RSPO2), each with their AbpromiseSM
Browse Abnova for Monoclonal and Polyclonal Antibodies
Search Novus for antibodies for RSPO2

Assays for RSPO2: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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2 InterPro domains/families:
 IPR000884 Thrombospondin_1_rpt
 IPR006212 Furin_repeat


   GeneDecks  RSPO2 for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry Q6UXX9

ProtoNet protein and cluster: Q6UXX9

1 Blocks protein family: IPB006212 Furin-like repeat

UniProtKB/Swiss-Prot: RSPO2_HUMAN, Q6UXX9
Domain: The FU repeat is required for activation and stabilization of beta-catenin (By similarity)
Similarity: Belongs to the R-spondin family
Similarity: Contains 1 FU (furin-like) repeat
Similarity: Contains 1 TSP type-1 domain

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (RSPO2)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_178565

              Applied Biosystems Silencer® siRNAs for RSPO2

              Sigma-Aldrich siRNA for RSPO2  
                     Sigma-Aldrich shRNA Panels and shRNA for RSPO2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for RSPO2
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_178565
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_178565
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_178565 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_178565

UniProtKB/Swiss-Prot: RSPO2_HUMAN, Q6UXX9
Function: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene
activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway, possibly via a direct
interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor
signaling pathway that may not use frizzled/LRP receptors. Probably also acts as a ligand for
frizzled and LRP receptors (By similarity)

13 MGI mutant phenotypes (inferred from 4 alleles(MGI details for Rspo2):

craniofacialdigestive/alimentaryembryogenesisendocrine/exocrine gland
homeostasis/metabolismlethality-prenatal/perinatallimbs/digits/tailrenal/urinary systemreproductive system
respiratory systemskeletonskin/coat/nails

1 Gene Ontology (GO) molecular function term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0008201 heparin binding IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section



3 Interacting proteins for RSPO2 (ENSP000002766593) via UniProtKB, MINT, and/or STRING
InteractantInteraction Details
GeneCardExternal ID(s)
FURINENSP000002681713STRING (score=.748)
WNT3AENSP000003557153STRING (score=.748)
LRP6ENSP000002613493STRING (score=.702)
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2 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0016055 Wnt receptor signaling pathway IEA--
GO:0050896 response to stimulus IEA--
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(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for RSPO2

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (RSPO2)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_178565

              Sigma-Aldrich siRNA for RSPO2  
                     Sigma-Aldrich shRNA Panels and shRNA for RSPO2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_178565  

REFSEQ mRNAs for RSPO2 gene: 

NM_178565.4   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_178565  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_178565
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_178565
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_178565 

Additional cDNA sequence: 

AK123023.1 AK123027.1 AY358166.1 BC027938.1 BC036554.1 

4 DOTS entries:

DT.40207933  DT.103816  DT.100674754  DT.120639542 

24/31 AceView cDNA sequences (see all 31 ):

BQ185494 D38717 AI417538 BU101597 AI656718 AI379477 AW160873 BX478040 
AA974429 NM_178565 AA723927 AK123027 AK123023 AW161744 AY358166 R52393 
BI912396 BI524005 BQ187856 AI016251 R52392 BC027938 BI496605 CB854676 

highest scoring ESTs for RSPO2:

AI656718 AA723927 AA974429 AI417538 AI797252 AK123023 AW160873 AW161137 AW183304 AY358166 

Unigene Cluster for RSPO2:

R-spondin 2 homolog (Xenopus laevis)
Hs.444834  [show with all ESTs]
Unigene Representative Sequence: NM_178565


GeneLoc Exon Structure

4 Alternative Splicing Database (ASD) splice patterns (SP) for RSPO2

ExUns: 1a · 1b · 1c ^ 2 ^ 3 ^ 4 ^ 5 ^ 6a · 6b ^ 7 ^ 8
SP1:              -           -     -                                 
SP2:                          -     -     -                           
SP3:                                -                                 
SP4:                                                                  

About this scheme

ECgene alternative splicing isoforms for RSPO2

2 Ensembl transcripts including schematic representations:
ENST00000276659  ENST00000378439  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

RSPO2 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for RSPO2

1 / 2 / 3

4 probe-sets matching RSPO2 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
82226_at2, 3 U95-D 1 1.00 1.00 0.92 0.96 AI417538 0.80 1.00 0.91 1

71702_at2, 3 U95-D 1 1.00 1.00 0.55 0.86 AI656718 1.00 1.00 1.00 1
64530_at2, 3 U95-C 1 1.00 1.00 0.96 1.19 AA723927 0.80 1.00 0.91 1

1554012_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  RSPO2 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
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Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: TCTATTTACA

SOURCE GeneReport for Unigene cluster: Hs.444834
(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for RSPO2 gene from 5/7 species (see all 7 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
RSPO21   -- R-spondin 2 homolog (Xenopus laevis) 95.06(n)
97.94(a)
482004  XM_539125.2  XP_539125.2 
chimpanzee
(Pan troglodytes)
RSPO21   -- R-spondin 2 homolog (Xenopus laevis) 99.86(n)
100(a)
464335  XM_001134914.1  XP_001134914.1 
cow
(Bos taurus)
RSPO21   -- R-spondin 2 homolog (Xenopus laevis) 94.79(n)
97.53(a)
536121  XM_616242.3  XP_616242.2 
rat
(Rattus norvegicus)
Rspo21   -- R-spondin 2 homolog (Xenopus laevis) 92.87(n)
95.88(a)
500863  XM_576261.2  XP_576261.1 
mouse
(Mus musculus)
Rspo21, 5 15 (17.11 cM)5
R-spondin 2 homolog (Xenopus laevis)1, 5 92.46(n)1
96.3(a)1
2394051  NM_172815.21  NP_766403.11 
 AA6732455  AA6734875  (see all 16)
About this table        Species with no ortholog for RSPO2

ENSEMBL Gene Tree for RSPO2
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for RSPO2 gene
RSPO32  RSPO12  RSPO42  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/993 NCBI SNPs in RSPO2 are shown (see all 993 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 309)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 8 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs5550081,2
C,F,H,O108981342(-) GCCTGT/GGCATA 1 -- ut3112Minor allele frequency- G:0.29MN NA EU EA WA 1164
rs3748101,2
A,C,F,H109165205(+) TTCCAG/AAGTGC 1 -- ng314Minor allele frequency- A:0.35EU EA WA 418
rs6015581,2
C,F,H109039543(+) GACACA/GGTATT 1 P/L mis1 ese312Minor allele frequency- G:0.48NA EU EA WA 1012
rs78267391,2
A,H109165927(+) TATGAT/CAGCAT 1 -- ng314Minor allele frequency- C:0.00EU EA WA 378
rs78261451,2
A,H109165522(+) TTTTGT/CTCATC 1 -- ng31 tfbs34Minor allele frequency- C:0.00EU EA WA 418
rs4781851,2
H109039486(+) TACCTC/TCTGGA 1 G/E mis14Minor allele frequency- T:0.00EU EA WA 420
--
rs78431161,2
A109165906(+) TGCCCA/GCAGTT 1 -- ng310--------
rs25148331,2
A,C,F,H,O109105371(+) AAAAAC/GCCAGG 1 -- int18Minor allele frequency- N:0.00EU EA WA NA 634
rs14345201,2
A,C,F,H,O109068799(+) AGCCTG/AGCAGG 1 -- int111Minor allele frequency- A:0.42EU EA WA NA 978
rs9892911,2
A,C,F,H,O109089576(-) AGTGCG/ATAAAC 1 -- int18Minor allele frequency- A:0.46EU EA WA 838
About this table

HapMap Linkage Disequilibrium images for RSPO2 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 610575

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

8 PubMed articles for RSPO2 gene:
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 340419 HGNC: 28583 AceView: MGC35555 Ensembl:ENSG00000147655 euGenes: HUgn340419
ECgene: RSPO2 H-InvDB: RSPO2
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
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  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for RSPO2:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for RSPO2-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for RSPO2 Browse antibodies at Sigma-Aldrich
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for RSPO2 Browse small molecules at Sigma-Aldrich
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