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Aliases for RSAD2 Gene

Aliases for RSAD2 Gene

  • Radical S-Adenosyl Methionine Domain Containing 2 2 3 5
  • Virus Inhibitory Protein, Endoplasmic Reticulum-Associated, Interferon-Inducible 3 4
  • Cytomegalovirus-Induced Gene 5 Protein 3 4
  • Viperin 3 4
  • Cig5 3 4
  • Radical S-Adenosyl Methionine Domain-Containing Protein 2 3
  • 2510004L01Rik 3
  • Cig33 3
  • Vig1 3

External Ids for RSAD2 Gene

Previous GeneCards Identifiers for RSAD2 Gene

  • GC02P006969
  • GC02P007005

Summaries for RSAD2 Gene

GeneCards Summary for RSAD2 Gene

RSAD2 (Radical S-Adenosyl Methionine Domain Containing 2) is a Protein Coding gene. Diseases associated with RSAD2 include Yellow Fever and Influenza. Among its related pathways are Interferon gamma signaling and Immune System. GO annotations related to this gene include protein self-association and iron-sulfur cluster binding.

UniProtKB/Swiss-Prot for RSAD2 Gene

  • Interferon-inducible iron-sulfur (4FE-4S) cluster-binding antiviral protein which plays a major role in the cell antiviral state induced by type I and type II interferon. Can inhibit a wide range of DNA and RNA viruses, including human cytomegalovirus (HCMV), hepatitis C virus (HCV), west Nile virus (WNV), dengue virus, sindbis virus, influenza A virus, sendai virus, vesicular stomatitis virus (VSV), and human immunodeficiency virus (HIV-1). Displays antiviral activity against influenza A virus by inhibiting the budding of the virus from the plasma membrane by disturbing the lipid rafts. This is accomplished, at least in part, through binding and inhibition of the enzyme farnesyl diphosphate synthase (FPPS), which is essential for the biosynthesis of isoprenoid-derived lipids. Promotes TLR7 and TLR9-dependent production of IFN-beta production in plasmacytoid dendritic cells (pDCs) by facilitating Lys-63-linked ubiquitination of IRAK1. Plays a role in CD4+ T-cells activation and differentiation. Facilitates T-cell receptor (TCR)-mediated GATA3 activation and optimal T-helper 2 (Th2) cytokine production by modulating NFKB1 and JUNB activities. Can inhibit secretion of soluble proteins.

No data available for Entrez Gene Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for RSAD2 Gene

Genomics for RSAD2 Gene

Regulatory Elements for RSAD2 Gene

Enhancers for RSAD2 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH02F006872 0.9 FANTOM5 Ensembl 28.5 +7.0 6995 0.8 JUND SIN3A RAD21 SPI1 RSAD2 CMPK2 GC02P006897
GH02F006858 0.6 FANTOM5 19.9 -7.3 -7317 0.4 ZNF707 RSAD2 CMPK2 NRIR
GH02F006946 0.7 FANTOM5 19.5 +81.1 81105 0.3 SMARCE1 JUN ARID1B JUND GATA3 IKZF1 FOS FOXJ2 RSAD2 RNF144A RNF144A-AS1 CMPK2 GC02M007018
GH02F007242 0.6 FANTOM5 Ensembl 14.2 +377.1 377095 1.4 IKZF1 HLF SPI1 RSAD2 CMPK2 GC02P007246 GC02M007162
GH02F006857 0.5 FANTOM5 13.3 -8.6 -8630 0.3 NFE2 RSAD2 CMPK2 NRIR
- Elite enhancer/Elite enhancer-gene association Download Table
Download GeneHancer data dump

Enhancers around RSAD2 on UCSC Golden Path with GeneCards custom track

Promoters for RSAD2 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00000675523 -106 2201 HDGF ATF1 ARNT CREB3L1 ZFP64 ZNF48 ELK1 GATA2 KLF13 REST

Genomic Location for RSAD2 Gene

Chromosome:
2
Start:
6,865,806 bp from pter
End:
6,898,239 bp from pter
Size:
32,434 bases
Orientation:
Plus strand

Genomic View for RSAD2 Gene

Genes around RSAD2 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
RSAD2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for RSAD2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RSAD2 Gene

Proteins for RSAD2 Gene

  • Protein details for RSAD2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q8WXG1-RSAD2_HUMAN
    Recommended name:
    Radical S-adenosyl methionine domain-containing protein 2
    Protein Accession:
    Q8WXG1
    Secondary Accessions:
    • Q8WVI4

    Protein attributes for RSAD2 Gene

    Size:
    361 amino acids
    Molecular mass:
    42170 Da
    Cofactor:
    Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
    Quaternary structure:
    • Homodimer. Interacts with IRAK1 and TRAF6 (By similarity). Interacts with FPPS. Interacts with human cytomegalovirus/HHV-5 protein vMIA/UL37; this interaction results in RSAD2/viperin relocalization from the endoplasmic reticulum to the mitochondria. Interacts with HADHB. Interacts (via C-terminus) with VAPA/VAP33 (via C-terminus) and inhibits its interaction with hepatitis virus C (HCV) protein NS5A.
    Miscellaneous:
    • Up-regulated in atherosclerosis. Latent viruses like HCMV may be involved in atherogenesis by initiating local inflammation. This may induce up-regulation of antiviral gene RSAD2, which modulates lipids synthesis, and thus could play a role in abnormal lipid accumulation leading to atherosclerosis.

neXtProt entry for RSAD2 Gene

Post-translational modifications for RSAD2 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for RSAD2 Gene

No data available for DME Specific Peptides for RSAD2 Gene

Domains & Families for RSAD2 Gene

Protein Domains for RSAD2 Gene

Suggested Antigen Peptide Sequences for RSAD2 Gene

Graphical View of Domain Structure for InterPro Entry

Q8WXG1

UniProtKB/Swiss-Prot:

RSAD2_HUMAN :
  • The N-terminal region (1-42) is necessary for its localization to the endoplasmic reticulum membrane and lipid droplet.
  • Belongs to the radical SAM superfamily. RSAD2 family.
Domain:
  • The N-terminal region (1-42) is necessary for its localization to the endoplasmic reticulum membrane and lipid droplet.
Family:
  • Belongs to the radical SAM superfamily. RSAD2 family.
genes like me logo Genes that share domains with RSAD2: view

No data available for Gene Families for RSAD2 Gene

Function for RSAD2 Gene

Molecular function for RSAD2 Gene

UniProtKB/Swiss-Prot Function:
Interferon-inducible iron-sulfur (4FE-4S) cluster-binding antiviral protein which plays a major role in the cell antiviral state induced by type I and type II interferon. Can inhibit a wide range of DNA and RNA viruses, including human cytomegalovirus (HCMV), hepatitis C virus (HCV), west Nile virus (WNV), dengue virus, sindbis virus, influenza A virus, sendai virus, vesicular stomatitis virus (VSV), and human immunodeficiency virus (HIV-1). Displays antiviral activity against influenza A virus by inhibiting the budding of the virus from the plasma membrane by disturbing the lipid rafts. This is accomplished, at least in part, through binding and inhibition of the enzyme farnesyl diphosphate synthase (FPPS), which is essential for the biosynthesis of isoprenoid-derived lipids. Promotes TLR7 and TLR9-dependent production of IFN-beta production in plasmacytoid dendritic cells (pDCs) by facilitating Lys-63-linked ubiquitination of IRAK1. Plays a role in CD4+ T-cells activation and differentiation. Facilitates T-cell receptor (TCR)-mediated GATA3 activation and optimal T-helper 2 (Th2) cytokine production by modulating NFKB1 and JUNB activities. Can inhibit secretion of soluble proteins.
UniProtKB/Swiss-Prot Induction:
By interferon type I, type II and bacterial lipopolysaccharides (LPS). Little or no induction by IFNG/IFN-gamma is observed in monocytic cell lines. Induced by infection with hepatitis C virus, yellow fever virus and Sendai virus, presumably through type I interferon pathway. Induction by infection with human cytomegalovirus (HCMV), stomatitis virus (VSV), chikungunya virus (CHIKV), Japanese encephalitis virus (JEV) occurs independent of the IFN pathway.

Gene Ontology (GO) - Molecular Function for RSAD2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003824 catalytic activity IEA --
GO:0005515 protein binding IPI 21527675
GO:0043621 protein self-association IDA 19074433
GO:0046872 metal ion binding IEA --
GO:0051536 iron-sulfur cluster binding IEA --
genes like me logo Genes that share ontologies with RSAD2: view
genes like me logo Genes that share phenotypes with RSAD2: view

Animal Models for RSAD2 Gene

MGI Knock Outs for RSAD2:

Animal Model Products

miRNA for RSAD2 Gene

miRTarBase miRNAs that target RSAD2

Inhibitory RNA Products

Clone Products

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for RSAD2 Gene

Localization for RSAD2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for RSAD2 Gene

Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus. Endoplasmic reticulum. Lipid droplet. Mitochondrion. Mitochondrion inner membrane. Mitochondrion outer membrane. Note=Infection with human cytomegalovirus (HCMV) causes relocation to the Golgi apparatus and to cytoplasmic vacuoles which also contain HCMV proteins glycoprotein B and pp28. Interaction with human cytomegalovirus/HHV-5 protein vMIA/UL37 results in its relocalization from the endoplasmic reticulum to the mitochondria.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for RSAD2 Gene COMPARTMENTS Subcellular localization image for RSAD2 gene
Compartment Confidence
endoplasmic reticulum 5
mitochondrion 5
golgi apparatus 4
cytosol 2
extracellular 1
nucleus 1

Gene Ontology (GO) - Cellular Components for RSAD2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005739 mitochondrion IEA,IDA 21527675
GO:0005741 mitochondrial outer membrane IEA,IDA 21527675
GO:0005743 mitochondrial inner membrane IEA,IDA 21527675
GO:0005783 endoplasmic reticulum IEA,IDA 11752458
GO:0005789 endoplasmic reticulum membrane TAS --
genes like me logo Genes that share ontologies with RSAD2: view

Pathways & Interactions for RSAD2 Gene

genes like me logo Genes that share pathways with RSAD2: view

Pathways by source for RSAD2 Gene

1 Cell Signaling Technology pathway for RSAD2 Gene
1 KEGG pathway for RSAD2 Gene

Gene Ontology (GO) - Biological Process for RSAD2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0009615 response to virus IDA 21957124
GO:0016032 viral process IEA --
GO:0034157 positive regulation of toll-like receptor 7 signaling pathway IEA,ISS --
GO:0034165 positive regulation of toll-like receptor 9 signaling pathway IEA,ISS --
GO:0035710 CD4-positive, alpha-beta T cell activation IEA,ISS --
genes like me logo Genes that share ontologies with RSAD2: view

No data available for SIGNOR curated interactions for RSAD2 Gene

Drugs & Compounds for RSAD2 Gene

(2) Drugs for RSAD2 Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
S-Adenosylmethionine Approved Nutra 0
heme Pharma Agonist 0

(1) Additional Compounds for RSAD2 Gene - From: Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
genes like me logo Genes that share compounds with RSAD2: view

Transcripts for RSAD2 Gene

Unigene Clusters for RSAD2 Gene

Radical S-adenosyl methionine domain containing 2:
Representative Sequences:

Inhibitory RNA Products

Clone Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for RSAD2 Gene

No ASD Table

Relevant External Links for RSAD2 Gene

GeneLoc Exon Structure for
RSAD2
ECgene alternative splicing isoforms for
RSAD2

Expression for RSAD2 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for RSAD2 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for RSAD2 Gene

This gene is overexpressed in Brain - Substantia nigra (x7.0).

Protein differential expression in normal tissues from HIPED for RSAD2 Gene

This gene is overexpressed in Lavage (69.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, and MOPED for RSAD2 Gene



Protein tissue co-expression partners for RSAD2 Gene

NURSA nuclear receptor signaling pathways regulating expression of RSAD2 Gene:

RSAD2

SOURCE GeneReport for Unigene cluster for RSAD2 Gene:

Hs.17518
genes like me logo Genes that share expression patterns with RSAD2: view

Primer Products

No data available for mRNA Expression by UniProt/SwissProt for RSAD2 Gene

Orthologs for RSAD2 Gene

This gene was present in the common ancestor of chordates.

Orthologs for RSAD2 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia RSAD2 34 35
  • 99.63 (n)
dog
(Canis familiaris)
Mammalia RSAD2 34 35
  • 80.89 (n)
mouse
(Mus musculus)
Mammalia Rsad2 34 16 35
  • 80.82 (n)
cow
(Bos Taurus)
Mammalia RSAD2 34 35
  • 80.7 (n)
rat
(Rattus norvegicus)
Mammalia Rsad2 34
  • 80.43 (n)
platypus
(Ornithorhynchus anatinus)
Mammalia RSAD2 35
  • 76 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia RSAD2 35
  • 72 (a)
OneToOne
chicken
(Gallus gallus)
Aves RSAD2 34 35
  • 76 (n)
lizard
(Anolis carolinensis)
Reptilia RSAD2 35
  • 73 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia rsad2 34
  • 66.67 (n)
zebrafish
(Danio rerio)
Actinopterygii rsad2 34 35
  • 67.76 (n)
Dr.5830 34
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.11692 34
Species where no ortholog for RSAD2 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for RSAD2 Gene

ENSEMBL:
Gene Tree for RSAD2 (if available)
TreeFam:
Gene Tree for RSAD2 (if available)

Paralogs for RSAD2 Gene

No data available for Paralogs for RSAD2 Gene

Variants for RSAD2 Gene

Sequence variations from dbSNP and Humsavar for RSAD2 Gene

SNP ID Clin Chr 02 pos Sequence Context AA Info Type
rs10182815 -- 6,877,772(+) TACAG(A/G)GACTG intron-variant, utr-variant-5-prime
rs10194045 -- 6,884,783(+) TGGAG(A/C)GCCAA intron-variant
rs10197837 -- 6,886,827(+) AAAAG(A/T)GTCTT intron-variant
rs10198816 -- 6,888,006(+) GCTTA(A/G)GTGGA intron-variant
rs1045239 -- 6,897,043(+) AGCCA(A/T)GTCAC utr-variant-3-prime

Structural Variations from Database of Genomic Variants (DGV) for RSAD2 Gene

Variant ID Type Subtype PubMed ID
nsv1123291 CNV deletion 24896259
nsv2596 CNV insertion 18451855
nsv833326 CNV loss 17160897

Variation tolerance for RSAD2 Gene

Residual Variation Intolerance Score: 67.9% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 1.80; 33.97% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for RSAD2 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
RSAD2

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for RSAD2 Gene

Disorders for RSAD2 Gene

MalaCards: The human disease database

(6) MalaCards diseases for RSAD2 Gene - From: DISEASES and GeneCards

Disorder Aliases PubMed IDs
yellow fever
  • jungle yellow fever
influenza
  • flu
encephalitis
west nile virus
  • west nile virus, susceptibility to
stomatitis
- elite association - COSMIC cancer census association via MalaCards
Search RSAD2 in MalaCards View complete list of genes associated with diseases

Relevant External Links for RSAD2

Genetic Association Database (GAD)
RSAD2
Human Genome Epidemiology (HuGE) Navigator
RSAD2
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
RSAD2
genes like me logo Genes that share disorders with RSAD2: view

No data available for UniProtKB/Swiss-Prot and Genatlas for RSAD2 Gene

Publications for RSAD2 Gene

  1. Viperin (cig5), an IFN-inducible antiviral protein directly induced by human cytomegalovirus. (PMID: 11752458) Chin K.-C. … Cresswell P. (Proc. Natl. Acad. Sci. U.S.A. 2001) 2 3 4 22 64
  2. Structural characterization reveals that viperin is a radical S- adenosyl-L-methionine (SAM) enzyme. (PMID: 20026307) Shaveta G. … Song J. (Biochem. Biophys. Res. Commun. 2010) 3 4 22 64
  3. The antiviral protein viperin is a radical SAM enzyme. (PMID: 20176015) Duschene K.S. … Broderick J.B. (FEBS Lett. 2010) 3 4 22 64
  4. The N-terminal amphipathic alpha-helix of viperin mediates localization to the cytosolic face of the endoplasmic reticulum and inhibits protein secretion. (PMID: 19074433) Hinson E.R. … Cresswell P. (J. Biol. Chem. 2009) 3 4 22 64
  5. Toll-like receptor-dependent and -independent viperin gene expression and counter-regulation by PRDI-binding factor-1/BLIMP1. (PMID: 16849320) Severa M. … Fitzgerald K.A. (J. Biol. Chem. 2006) 3 4 22 64

Products for RSAD2 Gene

Sources for RSAD2 Gene

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