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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

RPP14 Gene

protein-coding   GIFtS: 49
GCID: GC03P058267

ribonuclease P/MRP 14kDa subunit

(Previous name: ribonuclease P 14kDa subunit )
  Search for RPP14
in our new
 Human Malady Compendium 
Biological research products
for RPP14
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Ribonuclease P/MRP 14kDa Subunit1 2     3-Hydroxyacyl-[Acyl-Carrier-Protein] Dehydratase3
P141 2     EC 3.1.26.53
Ribonuclease P 14kDa Subunit1     EC 4.2.1.-3
Ribonuclease P Protein Subunit P142     HsHTD23

External Ids:    HGNC: 303271   Entrez Gene: 111022   Ensembl: ENSG000001636847   OMIM: 6061125   UniProtKB: P863973   
UniProtKB: O950593   

Export aliases for RPP14 gene to outside databases

Previous GC identifers: GC03M057092 GC03P057660 GC03P058146 GC03P058249 GC03P058224


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: RPP14_HUMAN, O95059
Function: Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends

Gene Wiki entry for RPP14


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000003.11  NC_018914.1  NT_022517.18  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the RPP14 gene promoter:
         E2F-4   E2F-3a   E2F-5   E2F-1   E2F   E2F-2   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidRPP14 promoter sequence
   Search SABiosciences Chromatin IP Primers for RPP14

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat RPP14


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 3p14.3   Ensembl cytogenetic band:  3p14.3   HGNC cytogenetic band: 3p21.2

RPP14 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
RPP14 gene location

GeneLoc information about chromosome 3         GeneLoc Exon Structure

GeneLoc location for GC03P058267:  view genomic region     (about GC identifiers)

Start:
58,291,972 bp from pter      End:
58,305,920 bp from pter
Size:
13,949 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: HTD2_HUMAN, P86397 (See protein sequence)
Recommended Name: Hydroxyacyl-thioester dehydratase type 2, mitochondrial precursor  
Size: 168 amino acids; 18559 Da
Subcellular location: Mitochondrion
Miscellaneous: This protein is produced by a bicistronic gene which also produces the RPP14 protein from an overlapping
reading frame
1 PDB 3D structure from and Proteopedia for RPP14:
3IR3 (3D)    

UniProtKB/Swiss-Prot: RPP14_HUMAN, O95059 (See protein sequence)

Recommended Name: Ribonuclease P protein subunit p14  
Size: 124 amino acids; 13693 Da
Subunit: RNase P consists of a RNA moiety and at least 8 protein subunits; POP1, RPP14, RPP20/POP7, RPP25, RPP29/POP4,
RPP30, RPP38 and RPP40
Subcellular location: Nucleus (Potential)
Miscellaneous: This protein is produced by a bicistronic gene which also produces the HTD2 protein from an overlapping
reading frame
Secondary accessions: Q53X97

Explore the universe of human proteins at neXtProt for RPP14: NX_O95059

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_O95059

  • RPP14 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins (2 alternative transcripts): 
    NP_001092253.1  NP_008973.1  

    ENSEMBL proteins: 
     ENSP00000412894   ENSP00000295959   ENSP00000419909  

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    Browse ProSpec Recombinant Proteins
    Uscn Proteins for RPP14

    Gene Ontology (GO): 1 cellular component term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IEA--


    RPP14 for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    RPP14 for domains           About GeneDecksing

    2 InterPro domains/families:
     IPR002539 MaoC_dom
     IPR002759 RNase_P/MRP_subunit

    Graphical View of Domain Structure for InterPro Entry P86397
    Graphical View of Domain Structure for InterPro Entry O95059

    ProtoNet protein and cluster: P86397

    UniProtKB/Swiss-Prot: HTD2_HUMAN, P86397
    Similarity: Belongs to the HTD2 family
    Similarity: Contains 1 MaoC-like domain

    UniProtKB/Swiss-Prot: RPP14_HUMAN, O95059
    Similarity: Belongs to the eukaryotic/archaeal RNase P protein component 2 family


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: HTD2_HUMAN, P86397
    Function: Mitochondrial 3-hydroxyacyl-thioester dehydratase, which may be involved in fatty acid biosynthesis
    Catalytic activity: (3R)-hydroxyacyl-CoA = trans-2-enoyl-CoA + H(2)O
    Catalytic activity: (3R)-hydroxyacyl-[acyl-carrier-protein] = trans-2-enoyl-[acyl-carrier-protein] + H(2)O

         UniProtKB/Swiss-Prot: RPP14_HUMAN, O95059
    Function: Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends
    Catalytic activity: Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor

    Enzyme Numbers (IUBMB): EC 3.1.26.51 EC 4.2.1.-1

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    Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003723RNA binding TAS10024167
    GO:0004526ribonuclease P activity IEA--


    RPP14 for ontologies           About GeneDecksing



    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways  About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1RNA transport
    RNA transport1.00

    Pathway sources
    See GeneCards unified pathways
    Show all pathways



    1         Kegg Pathway  (Kegg details for RPP14):
        RNA transport


    RPP14 for pathways           About GeneDecksing

    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for RPP14

    STRING Interaction Network Preview (showing 5 interactants - click image to see 13)

    5/23 Interacting proteins for RPP14 (O950592, 3 ENSP000002959594) via UniProtKB, MINT, STRING, and/or I2D (see all 23)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    IGSF21Q96ID52, 3, ENSP000002512964MINT-63243 I2D: score=4 STRING: ENSP00000251296
    NOVA1P515132, 3, ENSP000002674224MINT-8269175 I2D: score=2 STRING: ENSP00000267422
    A2MP010232, 3MINT-8269109 I2D: score=2 
    ATP5BP065762, 3MINT-8269120 I2D: score=2 
    CAPZBP477562, 3MINT-8269131 I2D: score=2 
    About this table

    Gene Ontology (GO): 1 biological process term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0008033tRNA processing IEA--


    RPP14 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for RPP14
    Search CenterWatch for drugs/clinical trials and news about RPP14 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
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    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for RPP14 gene (2 alternative transcripts): 
    NM_001098783.2  NM_007042.4  

    Unigene Cluster for RPP14:

    Ribonuclease P/MRP 14kDa subunit
    Hs.446320  [show with all ESTs]
    Unigene Representative Sequence: NM_001098783
    13 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000461797 ENST00000475412 ENST00000445193(uc010hnh.3 uc010hni.3)
    ENST00000474660 ENST00000477305 ENST00000295959 ENST00000466547 ENST00000481972
    ENST00000498618 ENST00000461393 ENST00000476007 ENST00000463550 ENST00000462046


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    hsa-miR-142-5p hsa-miR-2052 hsa-miR-4291 hsa-miR-596 hsa-miR-328 hsa-miR-19b-2* hsa-miR-3653 hsa-miR-507
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    Inhib. RNA
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    Additional cDNA sequence: 

    AF001175.1 AK056070.1 AK311396.1 AK311969.1 BC002441.1 BC007342.1 BC012017.1 CR407611.1 
    NR_049755.1 NR_049756.1 NR_049757.1 NR_049758.1 

    12 DOTS entries:

    DT.115264  DT.444287  DT.95270042  DT.95185112  DT.120918636  DT.120918643  DT.120918618  DT.95337769 
    DT.120918624  DT.65287248  DT.95196227  DT.95358966 

    24/264 AceView cDNA sequences (see all 264):

    AW769528 BC012017 BM476890 AA476338 AI249128 BE502834 AI093335 BU159844 
    AI469430 CB128667 AL561376 CR607469 AI671724 BQ101186 CR622586 AA580130 
    AI005368 BE671384 AA446296 AI682727 CA308551 AW613678 AI052194 AI693166 

    GeneLoc Exon Structure

    5/10 Alternative Splicing Database (ASD) splice patterns (SP) for RPP14 (see all 10)    About this scheme

    ExUns: 1a · 1b · 1c · 1d ^ 2a · 2b ^ 3 ^ 4a · 4b · 4c ^ 5 ^ 6a · 6b ^ 7a · 7b ^ 8 ^ 9a · 9b · 9c · 9d · 9e · 9f · 9g
    SP1:                          -     -     -     -     -                 -     -                                                               
    SP2:                          -     -     -     -     -     -           -                                                                     
    SP3:                                      -     -     -                 -     -                                                               
    SP4:                          -     -     -     -     -                                                                                       
    SP5:                          -     -     -     -     -     -           -     -                                                               


    ECgene alternative splicing isoforms for RPP14

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    RPP14 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: TATCAGTACA

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See RPP14 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for RPP14

    SOURCE GeneReport for Unigene cluster: Hs.446320

    UniProtKB/Swiss-Prot: HTD2_HUMAN, P86397
    Tissue specificity: Highly expressed in heart and liver. Expressed at lower levels in skeletal muscle, spleen, kidney
    and placenta


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    In Situ
    Assay Products:
     

     
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of chordates.

    Orthologs for RPP14 gene from 5/14 species (see all 14)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Rpp141 , 5 ribonuclease P 14 subunit (human)5
    ribonuclease P 14 subunit1
    82.92(n)1
    85.95(a)1
      14 (4.70 cM)5
    670531  NM_025938.41  NP_080214.11 
     80803135 
    chicken
    (Gallus gallus)
    Aves RPP141 ribonuclease P/MRP 14kDa subunit 62.5(n)
    60.83(a)
      416064  XM_414402.3  XP_414402.2 
    lizard
    (Anolis carolinensis)
    Reptilia RPP146
    --
    66(a)
    1 ↔ 1
    2(187318639-187323381)
    African clawed frog
    (Xenopus laevis)
    Amphibia Xl.82042 Xenopus laevis transcribed sequence with weak similarity more 73.61(n)    BJ047180.1 
    zebrafish
    (Danio rerio)
    Actinopterygii BM186106.12   -- 72.34(n)    BM186106.1 


    ENSEMBL Gene Tree for RPP14 (if available)
    TreeFam Gene Tree for RPP14 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
      --

    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/363 NCBI SNPs in RPP14 are shown (see all 363    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 3 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs600957721,2
    C,F,--58289973(+) GGTCTG/ATGGGG 6 -- us2k17Minor allele frequency- A:0.46WA NA CSA EA 365
    rs677931961,2
    C,F,--58290129(+) GAAAAT/GAATTG 6 -- us2k16Minor allele frequency- G:0.47WA NA CSA EA 363
    rs130948981,2
    C,F,H,--58290143(+) AAGGAG/AATCCC 6 -- us2k1 trp317Minor allele frequency- A:0.06NS EA NA CSA WA 2134
    rs1870116691,2
    --58290150(+) TCCCCA/GCTGAC 6 -- us2k10--------
    rs622593191,2
    C,F,--58290358(+) CTCAAT/CTGGTG 6 -- us2k16Minor allele frequency- C:0.47WA NA CSA EA 363
    rs1910485991,2
    --58290430(+) ACTGTA/GATCTT 6 -- us2k10--------
    rs1833639171,2
    --58290442(+) AGCAAA/GTAACT 6 -- us2k10--------
    rs1872856921,2
    --58290509(+) GATCTC/TGGCTT 6 -- us2k10--------
    rs622593201,2
    C,F,--58290629(+) AGAGAC/TGGGGG 6 -- us2k13Minor allele frequency- T:0.40NA CSA 5
    rs1913752401,2
    --58290645(+) CCATGC/TTGGCC 6 -- us2k10--------

    HapMap Linkage Disequilibrium report for RPP14 (58291972 - 58305920 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for RPP14: --

    SABiosciences Cancer Mutation PCR Assays
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    RPP14 for disorders           About MalaCards

    RPP14 for disorders           About GeneDecksing

    OMIM gene information: 606112    OMIM disorders: --


    Export disorders for RPP14 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for RPP14 gene, integrated from 9 sources (see all 21):
    (articles sorted by number of sources associating them with RPP14)
        Utopia: connect your pdf to the dynamic
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    1. Rpp14 and Rpp29, two protein subunits of human ribonuclease P. (PubMed id 10024167)1, 2, 3, 9 Jarrous N.... Altman S. (1999)
    2. A protein subunit of human RNase P, Rpp14, and its interacting partner, OIP2, have 3'-->5' exoribonuclease activity. (PubMed id 11929972)1, 3, 9 Jiang T. and Altman S. (2002)
    3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    4. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
    5. Functional dissection of lysine deacetylases reveals t hat HDAC1 and p300 regulate AMPK. (PubMed id 22318606)1 Lin Y.Y....Boeke J.D. (2012)
    6. Initial characterization of the human central proteome. (PubMed id 21269460)2 Burkard T.R.... Colinge J. (2011)
    7. A directed protein interaction network for investigat ing intracellular signal transduction. (PubMed id 21900206)1 Vinayagam A....Wanker E.E. (2011)
    8. Mass spectrometric analysis of lysine ubiquitylation r eveals promiscuity at site level. (PubMed id 21139048)1 Danielsen J.M....Nielsen M.L. (2011)
    9. Ubiquitin-mediated proteolysis of HuR by heat shock. (PubMed id 19322201)1 Abdelmohsen K....Gorospe M. (2009)
    10. An ancient genetic link between vertebrate mitochondrial fatty acid synthesis and RNA processing. (PubMed id 17898086)2 Autio K.J.... Hiltunen J.K. (2008)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 11102 HGNC: 30327 AceView: RPP14 Ensembl:ENSG00000163684 euGenes: HUgn11102
    ECgene: RPP14 Kegg: 11102 H-InvDB: RPP14

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for RPP14 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for RPP14 gene:
    Search GeneIP for patents involving RPP14

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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