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Aliases for RPN2 Gene

Aliases for RPN2 Gene

  • Ribophorin II 2 3 4
  • Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase 63 KDa Subunit 3 4
  • Oligosaccharyltransferase Complex Subunit (Non-Catalytic) 2 3
  • Ribophorin-2 3 4
  • RIBIIR 3 4
  • RPN-II 3 4
  • Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase Subunit 2 3
  • EC 2.4.1.119 63
  • EC 2.4.99.18 4
  • RPNII 3
  • SWP1 3

External Ids for RPN2 Gene

Previous GeneCards Identifiers for RPN2 Gene

  • GC20P035535
  • GC20P036445
  • GC20P036492
  • GC20P035240
  • GC20P035806
  • GC20P032542

Summaries for RPN2 Gene

Entrez Gene Summary for RPN2 Gene

  • This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein is similar in sequence to the yeast oligosaccharyl transferase subunit SWP1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]

GeneCards Summary for RPN2 Gene

RPN2 (Ribophorin II) is a Protein Coding gene. Among its related pathways are Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein and Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein. GO annotations related to this gene include ribosome binding and dolichyl-diphosphooligosaccharide-protein glycotransferase activity.

UniProtKB/Swiss-Prot for RPN2 Gene

  • Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains

Gene Wiki entry for RPN2 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for RPN2 Gene

Genomics for RPN2 Gene

Regulatory Elements for RPN2 Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for RPN2 Gene

Start:
37,178,410 bp from pter
End:
37,241,623 bp from pter
Size:
63,214 bases
Orientation:
Plus strand

Genomic View for RPN2 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for RPN2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RPN2 Gene

Proteins for RPN2 Gene

  • Protein details for RPN2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P04844-RPN2_HUMAN
    Recommended name:
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2
    Protein Accession:
    P04844
    Secondary Accessions:
    • Q5JYR6
    • Q6IBA5
    • Q96E21
    • Q9BUQ3
    • Q9UBE1

    Protein attributes for RPN2 Gene

    Size:
    631 amino acids
    Molecular mass:
    69284 Da
    Quaternary structure:
    • Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes. Also identified as part of a complex which includes CANX, DERL1, DERL2, DDOST/OST48, RPN1, RPN2, SELK, VIMP, STT3A AND VCP. This contains known members of the OST complex and may be a form of this complex.

    Alternative splice isoforms for RPN2 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for RPN2 Gene

Proteomics data for RPN2 Gene at MOPED

Post-translational modifications for RPN2 Gene

  • Ubiquitination at Lys154 and Lys627
  • Glycosylation at Asn106
  • Modification sites at PhosphoSitePlus

Other Protein References for RPN2 Gene

Antibody Products

Domains for RPN2 Gene

Protein Domains for RPN2 Gene

InterPro:
ProtoNet:

Graphical View of Domain Structure for InterPro Entry

P04844

UniProtKB/Swiss-Prot:

RPN2_HUMAN :
  • P04844
Family:
  • Belongs to the SWP1 family.
genes like me logo Genes that share domains with RPN2: view

No data available for Gene Families for RPN2 Gene

Function for RPN2 Gene

Molecular function for RPN2 Gene

GENATLAS Biochemistry: ribophorin II,69.5kDa,component with RPN1 and DDOST of N-oligosacccharyl transferase
UniProtKB/Swiss-Prot CatalyticActivity: Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine
UniProtKB/Swiss-Prot Function: Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains

Enzyme Numbers (IUBMB) for RPN2 Gene

Gene Ontology (GO) - Molecular Function for RPN2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004579 contributes_to dolichyl-diphosphooligosaccharide-protein glycotransferase activity NAS 15835887
GO:0043022 ribosome binding IEA --
genes like me logo Genes that share ontologies with RPN2: view

Phenotypes for RPN2 Gene

MGI mutant phenotypes for RPN2:
inferred from 1 alleles
GenomeRNAi human phenotypes for RPN2:
genes like me logo Genes that share phenotypes with RPN2: view

No data available for Animal Models , Transcription Factor Targeting and HOMER Transcription for RPN2 Gene

Localization for RPN2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for RPN2 Gene

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for RPN2 Gene COMPARTMENTS Subcellular localization image for RPN2 gene
Compartment Confidence
endoplasmic reticulum 5
nucleus 4
extracellular 2
vacuole 2
golgi apparatus 1
lysosome 1
peroxisome 1
plasma membrane 1

Gene Ontology (GO) - Cellular Components for RPN2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000421 autophagic vacuole membrane IEA --
GO:0005634 nucleus IDA --
GO:0005783 endoplasmic reticulum IDA --
GO:0005789 endoplasmic reticulum membrane TAS --
GO:0005791 rough endoplasmic reticulum IEA --
genes like me logo Genes that share ontologies with RPN2: view

Pathways for RPN2 Gene

genes like me logo Genes that share pathways with RPN2: view

UniProtKB/Swiss-Prot P04844-RPN2_HUMAN

  • Pathway: Protein modification; protein glycosylation

Gene Ontology (GO) - Biological Process for RPN2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006412 translation TAS --
GO:0006464 cellular protein modification process TAS 10660554
GO:0006487 protein N-linked glycosylation --
GO:0006614 SRP-dependent cotranslational protein targeting to membrane TAS --
GO:0007568 aging IEA --
genes like me logo Genes that share ontologies with RPN2: view

Compounds for RPN2 Gene

(2) HMDB Compounds for RPN2 Gene

Compound Synonyms Cas Number PubMed IDs
Dolichyl diphosphate
  • Dolichyl diphosphate
37247-98-6
L-Asparagine
  • (-)-Asparagine
70-47-3
genes like me logo Genes that share compounds with RPN2: view

Transcripts for RPN2 Gene

Unigene Clusters for RPN2 Gene

Ribophorin II:
Representative Sequences:

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for RPN2

Primer Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for RPN2 Gene

ExUns: 1 ^ 2a · 2b · 2c ^ 3 ^ 4 ^ 5a · 5b ^ 6 ^ 7 ^ 8 ^ 9 ^ 10a · 10b ^ 11a · 11b · 11c ^ 12 ^ 13 ^ 14a · 14b ^ 15 ^ 16a · 16b ^ 17a · 17b ·
SP1: - - - - - - -
SP2: - - - - - - - -
SP3: - - - -
SP4: -
SP5: - - - -
SP6: - -
SP7: - - -
SP8: - -
SP9:
SP10:
SP11:
SP12:

ExUns: 17c ^ 18 ^ 19a · 19b ^ 20 ^ 21
SP1:
SP2:
SP3:
SP4:
SP5:
SP6:
SP7:
SP8: -
SP9:
SP10:
SP11:
SP12:

Relevant External Links for RPN2 Gene

GeneLoc Exon Structure for
RPN2
ECgene alternative splicing isoforms for
RPN2

Expression for RPN2 Gene

mRNA expression in normal human tissues for RPN2 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for RPN2 Gene

SOURCE GeneReport for Unigene cluster for RPN2 Gene Hs.370895

mRNA Expression by UniProt/SwissProt for RPN2 Gene

P04844-RPN2_HUMAN
Tissue specificity: Expressed in all tissues tested
genes like me logo Genes that share expressions with RPN2: view

In Situ Assay Products

No data available for mRNA differential expression in normal tissues for RPN2 Gene

Orthologs for RPN2 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for RPN2 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia RPN2 35
  • 99.26 (n)
  • 99.68 (a)
RPN2 36
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia RPN2 35
  • 91.34 (n)
  • 95.4 (a)
RPN2 36
  • 95 (a)
OneToOne
dog
(Canis familiaris)
Mammalia RPN2 35
  • 91.8 (n)
  • 95.71 (a)
RPN2 36
  • 97 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Rpn2 35
  • 88.38 (n)
  • 92.39 (a)
Rpn2 16
Rpn2 36
  • 92 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia RPN2 36
  • 83 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Rpn2 35
  • 89.43 (n)
  • 93.03 (a)
chicken
(Gallus gallus)
Aves RPN2 35
  • 75.83 (n)
  • 78.22 (a)
RPN2 36
  • 78 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia RPN2 36
  • 80 (a)
OneToOne
African clawed frog
(Xenopus laevis)
Amphibia MGC53764 35
tropical clawed frog
(Silurana tropicalis)
Amphibia rpn2 35
  • 69.27 (n)
  • 73.41 (a)
Str.9570 35
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.1730 35
zebrafish
(Danio rerio)
Actinopterygii Dr.4217 35
rpn2 35
  • 66.83 (n)
  • 67.64 (a)
rpn2 36
  • 59 (a)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP007638 35
  • 44.55 (n)
  • 32.68 (a)
fruit fly
(Drosophila melanogaster)
Insecta CG6370 36
  • 30 (a)
OneToOne
OstDelta 35
  • 45.96 (n)
  • 35.88 (a)
CG6370 37
  • 33 (a)
worm
(Caenorhabditis elegans)
Secernentea ostd-1 35
  • 48.85 (n)
  • 39.27 (a)
ostd-1 36
  • 38 (a)
OneToOne
M01A10.3 37
  • 36 (a)
thale cress
(Arabidopsis thaliana)
eudicotyledons HAP6 35
  • 43.43 (n)
  • 29.17 (a)
rice
(Oryza sativa)
Liliopsida Os01g0911200 35
  • 43.51 (n)
  • 29.87 (a)
sea squirt
(Ciona savignyi)
Ascidiacea CSA.10212 36
  • 33 (a)
OneToOne
Species with no ortholog for RPN2:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for RPN2 Gene

ENSEMBL:
Gene Tree for RPN2 (if available)
TreeFam:
Gene Tree for RPN2 (if available)

Paralogs for RPN2 Gene

No data available for Paralogs for RPN2 Gene

Variants for RPN2 Gene

Sequence variations from dbSNP and Humsavar for RPN2 Gene

SNP ID Clin Chr 20 pos Sequence Context AA Info Type MAF
rs4608 -- 37,236,651(+) CCATC(C/T)TGGGC reference, synonymous-codon
rs761013 -- 37,232,704(-) AGCAT(C/T)GGATC intron-variant
rs765630 -- 37,222,593(+) TGCCA(A/G)AGCTA intron-variant
rs879818 -- 37,226,621(-) CCCAC(A/G)AATAC intron-variant
rs882562 -- 37,231,397(+) AAATA(C/T)CATCA intron-variant

Structural Variations from Database of Genomic Variants (DGV) for RPN2 Gene

Variant ID Type Subtype PubMed ID
esv2516589 CNV Deletion 19546169
esv2082246 CNV Deletion 18987734
esv8416 CNV Loss 19470904
esv2722385 CNV Deletion 23290073
esv4013 CNV Deletion 18987735
esv2663037 CNV Deletion 23128226
esv1174249 CNV Deletion 17803354
nsv519722 CNV Loss 19592680

Relevant External Links for RPN2 Gene

HapMap Linkage Disequilibrium report
RPN2
Human Gene Mutation Database (HGMD)
RPN2

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for RPN2 Gene

Disorders for RPN2 Gene

(1) Novoseek inferred disease relationships for RPN2 Gene

Disease -log(P) Hits PubMed IDs
tumors 0 1

Relevant External Links for RPN2

Genetic Association Database (GAD)
RPN2
Human Genome Epidemiology (HuGE) Navigator
RPN2
genes like me logo Genes that share disorders with RPN2: view

No data available for UniProtKB/Swiss-Prot for RPN2 Gene

Publications for RPN2 Gene

  1. Mapping of the ribophorin II (RPN II) gene to human chromosome 20q12-q13.1 by in-situ hybridization. (PMID: 2066112) LAPffler C. … Hansmann I. (Hum. Genet. 1991) 3 23
  2. Dinucleotide repeat polymorphism at the human ribophorin II locus (RPN2) on chromosome 20q. (PMID: 1301181) Stoffel M. … Bell G.I. (Hum. Mol. Genet. 1992) 3 23
  3. Interleukin-2 induces N-glycosylation in T-cells: characterization of human lymphocyte oligosaccharyltransferase. (PMID: 9642163) Kumar V. … Ozols J. (Biochem. Biophys. Res. Commun. 1998) 3 23
  4. Retention of subunits of the oligosaccharyltransferase complex in the endoplasmic reticulum. (PMID: 10660554) Fu J. … Kreibich G. (J. Biol. Chem. 2000) 3 23
  5. Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides. (PMID: 12665801) Gevaert K. … Vandekerckhove J. (Nat. Biotechnol. 2003) 3 4

Products for RPN2 Gene

  • Addgene plasmids for RPN2

Sources for RPN2 Gene

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