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Aliases for RPA4 Gene

Aliases for RPA4 Gene

  • Replication Protein A4, 30kDa 2 3
  • Replication Factor A Protein 4 3 4
  • Replication Protein A4, 34kDa 2 3
  • RF-A Protein 4 3 4
  • RP-A P30 3 4
  • Replication Protein A Complex 34 Kd Subunit Homolog Rpa4 3
  • Replication Protein A 30 KDa Subunit 3
  • HSU24186 3

External Ids for RPA4 Gene

Previous GeneCards Identifiers for RPA4 Gene

  • GC0XP095945
  • GC0XP096139
  • GC0XP085939

Summaries for RPA4 Gene

Entrez Gene Summary for RPA4 Gene

  • This gene encodes a single-stranded DNA-binding protein that is the 30-kDa subunit of the replication protein A complex. Replication protein A is an essential factor for DNA double-strand break repair and cell cycle checkpoint activation. The encoded protein localizes to DNA repair foci and may be involved in the cellular DNA damage response. This protein may also play a role in inhibiting viral replication.[provided by RefSeq, Apr 2010]

GeneCards Summary for RPA4 Gene

RPA4 (Replication Protein A4, 30kDa) is a Protein Coding gene. Diseases associated with RPA4 include babesiosis and lung cancer. Among its related pathways are Cell Cycle, Mitotic and Cell Cycle, Mitotic. GO annotations related to this gene include single-stranded DNA binding. An important paralog of this gene is RPA2.

UniProtKB/Swiss-Prot for RPA4 Gene

  • As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

Gene Wiki entry for RPA4 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for RPA4 Gene

Genomics for RPA4 Gene

Regulatory Elements for RPA4 Gene

Genomic Location for RPA4 Gene

Start:
96,883,908 bp from pter
End:
96,885,467 bp from pter
Size:
1,560 bases
Orientation:
Plus strand

Genomic View for RPA4 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for RPA4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RPA4 Gene

Proteins for RPA4 Gene

  • Protein details for RPA4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q13156-RFA4_HUMAN
    Recommended name:
    Replication protein A 30 kDa subunit
    Protein Accession:
    Q13156
    Secondary Accessions:
    • Q3SY03

    Protein attributes for RPA4 Gene

    Size:
    261 amino acids
    Molecular mass:
    28868 Da
    Quaternary structure:
    • Component of the aRPA, the alternative replication protein A complex, a trimeric complex similar to the replication protein A complex/RPA but where RPA1 and RPA3 are associated with RPA4 instead of RPA2. Interacts with RPA1 and RPA3.

neXtProt entry for RPA4 Gene

Proteomics data for RPA4 Gene at MOPED

Post-translational modifications for RPA4 Gene

No Post-translational modifications

Other Protein References for RPA4 Gene

ENSEMBL proteins:
Reactome Protein details:
REFSEQ proteins:

No data available for DME Specific Peptides for RPA4 Gene

Domains for RPA4 Gene

Protein Domains for RPA4 Gene

Suggested Antigen Peptide Sequences for RPA4 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q13156

UniProtKB/Swiss-Prot:

RFA4_HUMAN :
  • Q13156
Domain:
  • Contains 1 OB DNA-binding domain.
Family:
  • Belongs to the replication factor A protein 2 family.
genes like me logo Genes that share domains with RPA4: view

No data available for Gene Families for RPA4 Gene

Function for RPA4 Gene

Molecular function for RPA4 Gene

UniProtKB/Swiss-Prot Function: As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

Gene Ontology (GO) - Molecular Function for RPA4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003676 nucleic acid binding --
GO:0003677 DNA binding --
GO:0003697 single-stranded DNA binding TAS 7760808
genes like me logo Genes that share ontologies with RPA4: view

No data available for Enzyme Numbers (IUBMB) , Phenotypes , Animal Models , miRNA , Transcription Factor Targeting and HOMER Transcription for RPA4 Gene

Localization for RPA4 Gene

Subcellular locations from UniProtKB/Swiss-Prot for RPA4 Gene

Nucleus. Note=Localizes to DNA repair foci after DNA damage.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for RPA4 Gene COMPARTMENTS Subcellular localization image for RPA4 gene
Compartment Confidence
nucleus 5
cytosol 2

Gene Ontology (GO) - Cellular Components for RPA4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IDA 19942684
GO:0005654 nucleoplasm TAS --
GO:0005662 DNA replication factor A complex IDA 19116208
genes like me logo Genes that share ontologies with RPA4: view

Pathways for RPA4 Gene

genes like me logo Genes that share pathways with RPA4: view

Gene Ontology (GO) - Biological Process for RPA4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000077 DNA damage checkpoint IDA 19942684
GO:0000082 G1/S transition of mitotic cell cycle TAS --
GO:0000278 mitotic cell cycle TAS --
GO:0006260 DNA replication --
GO:0006270 DNA replication initiation TAS 7760808
genes like me logo Genes that share ontologies with RPA4: view

Compounds for RPA4 Gene

(1) Novoseek inferred chemical compound relationships for RPA4 Gene

Compound -log(P) Hits PubMed IDs
rpa 2 85.3 4
genes like me logo Genes that share compounds with RPA4: view

Transcripts for RPA4 Gene

mRNA/cDNA for RPA4 Gene

(6) Selected AceView cDNA sequences:
(6) Additional mRNA sequences :
(1) REFSEQ mRNAs :
(1) Ensembl transcripts including schematic representations, and UCSC links where relevant :

Unigene Clusters for RPA4 Gene

Replication protein A4, 30kDa:
Representative Sequences:

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for RPA4

Primer Products

  • OriGene qSTAR qPCR primer pairs in human,mouse,rat for RPA4
  • QuantiTect SYBR Green Assays in human,mouse,rat
  • Pre-validated RT² qPCR Primer Assay in human,mouse,rat
  • QuantiFast Probe-based Assays in human,mouse,rat

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for RPA4 Gene

No ASD Table

Relevant External Links for RPA4 Gene

GeneLoc Exon Structure for
RPA4
ECgene alternative splicing isoforms for
RPA4

Expression for RPA4 Gene

mRNA expression in normal human tissues for RPA4 Gene

mRNA differential expression in normal tissues according to GTEx for RPA4 Gene

This gene is overexpressed in Testis (8.9).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, and MOPED for RPA4 Gene

SOURCE GeneReport for Unigene cluster for RPA4 Gene Hs.659349

mRNA Expression by UniProt/SwissProt for RPA4 Gene

Q13156-RFA4_HUMAN
Tissue specificity: Preferentially expressed in placental and colon mucosa. Widely expressed at intermediate or lower levels.
genes like me logo Genes that share expressions with RPA4: view

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery for RPA4 Gene

Orthologs for RPA4 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for RPA4 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia RPA4 35
  • 99.49 (n)
  • 98.85 (a)
dog
(Canis familiaris)
Mammalia RPA4 36
  • 49 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia -- 36
  • 42 (a)
ManyToMany
-- 36
  • 42 (a)
ManyToMany
platypus
(Ornithorhynchus anatinus)
Mammalia -- 36
  • 34 (a)
OneToMany
chicken
(Gallus gallus)
Aves RPA2 36
  • 38 (a)
OneToMany
lizard
(Anolis carolinensis)
Reptilia -- 36
  • 37 (a)
OneToMany
zebrafish
(Danio rerio)
Actinopterygii rpa2 36
  • 35 (a)
OneToMany
fruit fly
(Drosophila melanogaster)
Insecta RPA2 36
  • 21 (a)
OneToMany
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes RFA2 36
  • 19 (a)
OneToMany
sea squirt
(Ciona savignyi)
Ascidiacea CSA.6662 36
  • 25 (a)
OneToMany
Species with no ortholog for RPA4:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • cow (Bos Taurus)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • mouse (Mus musculus)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rat (Rattus norvegicus)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for RPA4 Gene

ENSEMBL:
Gene Tree for RPA4 (if available)
TreeFam:
Gene Tree for RPA4 (if available)

Paralogs for RPA4 Gene

Paralogs for RPA4 Gene

Selected SIMAP similar genes for RPA4 Gene using alignment to 1 proteins:

genes like me logo Genes that share paralogs with RPA4: view

Variants for RPA4 Gene

Sequence variations from dbSNP and Humsavar for RPA4 Gene

SNP ID Clin Chr 0X pos Sequence Context AA Info Type MAF
VAR_019170 -

Structural Variations from Database of Genomic Variants (DGV) for RPA4 Gene

Variant ID Type Subtype PubMed ID
dgv520n21 CNV Gain 19592680

Relevant External Links for RPA4 Gene

HapMap Linkage Disequilibrium report
RPA4
Human Gene Mutation Database (HGMD)
RPA4
Locus Specific Mutation Databases (LSDB)
RPA4

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for RPA4 Gene

Disorders for RPA4 Gene

MalaCards: The human disease database

MalaCards: The human disease database. (2) Diseases for RPA4 Gene including...

Search for RPA4 Gene in MalaCards »

(1) University of Copenhagen DISEASES for RPA4 Gene

Relevant External Links for RPA4

Genetic Association Database (GAD)
RPA4
Human Genome Epidemiology (HuGE) Navigator
RPA4
genes like me logo Genes that share disorders with RPA4: view

No data available for UniProtKB/Swiss-Prot for RPA4 Gene

Publications for RPA4 Gene

  1. Rpa4, a homolog of the 34-kilodalton subunit of the replication protein A complex. (PMID: 7760808) Keshav K.F. … Dutta A. (Mol. Cell. Biol. 1995) 2 3 4 23
  2. An alternative form of replication protein a prevents viral replication in vitro. (PMID: 19116208) Mason A.C. … Wold M.S. (J. Biol. Chem. 2009) 3 4 23
  3. A naturally occurring human RPA subunit homolog does not support DNA replication or cell-cycle progression. (PMID: 19942684) Haring S.J. … Wold M.S. (Nucleic Acids Res. 2010) 3 4 23
  4. An alternative form of replication protein a expressed in normal human tissues supports DNA repair. (PMID: 19996105) Kemp M.G. … Wold M.S. (J. Biol. Chem. 2010) 3 4 23
  5. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard D.S. … Malek J. (Genome Res. 2004) 3 4

Products for RPA4 Gene

Sources for RPA4 Gene

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