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Aliases for RPA3 Gene

Aliases for RPA3 Gene

  • Replication Protein A3 2 3 5
  • Replication Factor A Protein 3 3 4
  • Replication Protein A3, 14kDa 2 3
  • RF-A Protein 3 3 4
  • RP-A P14 3 4
  • REPA3 3 4
  • Replication Protein A 14 KDa Subunit 3
  • Replication Protein A3 (14kD) 2
  • RPA14 4

External Ids for RPA3 Gene

Previous GeneCards Identifiers for RPA3 Gene

  • GC07M007320
  • GC07M007420
  • GC07M007449

Summaries for RPA3 Gene

GeneCards Summary for RPA3 Gene

RPA3 (Replication Protein A3) is a Protein Coding gene. Diseases associated with RPA3 include Epidemic Typhus and Ovary Serous Adenocarcinoma. Among its related pathways are Mismatch repair and DNA Double-Strand Break Repair. GO annotations related to this gene include single-stranded DNA binding and damaged DNA binding.

UniProtKB/Swiss-Prot for RPA3 Gene

  • As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage (PubMed:9430682). In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response (PubMed:24332808). It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin, in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair (PubMed:7697716). Plays also a role in base excision repair (BER), probably through interaction with UNG (PubMed:9765279). Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA3 has its own single-stranded DNA-binding activity and may be responsible for polarity of the binding of the complex to DNA (PubMed:19010961). As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange (PubMed:19996105).

Gene Wiki entry for RPA3 Gene

Additional gene information for RPA3 Gene

No data available for Entrez Gene Summary , CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for RPA3 Gene

Genomics for RPA3 Gene

Regulatory Elements for RPA3 Gene

Enhancers for RPA3 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH07H007639 1.4 ENCODE dbSUPER 25.8 +77.6 77582 3 HDGF PKNOX1 FOXA2 ARNT ZFP64 ARID4B SIN3A DMAP1 ZNF2 ZBTB7B RPA3 COL28A1 ENSG00000272732 ENSG00000283549
GH07H007562 2 FANTOM5 Ensembl ENCODE dbSUPER 13.8 +151.7 151712 8 HDGF PKNOX1 FOXA2 SIN3A FEZF1 YBX1 ZNF2 YY1 ZNF143 ZNF207 ZNF12 C7orf26 ZNF316 ENSG00000272894 LOC100421597 COL28A1 RPA3 MIOS CCZ1B ENSG00000198580
GH07H007438 1 Ensembl ENCODE 14.6 +279.4 279360 1 SMARCC1 FOXA2 NFIB NFIA JUN CEBPB EP300 RFX5 FOXA1 JUND RPA3 ENSG00000272894 COL28A1 GC07M007325
GH07H007424 1.1 Ensembl ENCODE 12.6 +294.0 294013 1 CTCF TFAP4 PKNOX1 ATF1 RB1 ARNT MAX RAD21 RFX5 YY1 RPA3 GC07M007325 COL28A1
GH07H007718 1.2 Ensembl ENCODE 10.7 -1.3 -1298 2 TBP PKNOX1 BATF RFX5 ZNF366 RCOR1 ATF7 FOS RUNX3 CREM RPA3 UMAD1 COL28A1 ENSG00000272894 LOC100533631
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around RPA3 on UCSC Golden Path with GeneCards custom track

Genomic Location for RPA3 Gene

Chromosome:
7
Start:
7,636,518 bp from pter
End:
7,718,607 bp from pter
Size:
82,090 bases
Orientation:
Minus strand

Genomic View for RPA3 Gene

Genes around RPA3 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
RPA3 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for RPA3 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RPA3 Gene

Proteins for RPA3 Gene

  • Protein details for RPA3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P35244-RFA3_HUMAN
    Recommended name:
    Replication protein A 14 kDa subunit
    Protein Accession:
    P35244
    Secondary Accessions:
    • Q549U6

    Protein attributes for RPA3 Gene

    Size:
    121 amino acids
    Molecular mass:
    13569 Da
    Quaternary structure:
    • Component of the canonical replication protein A complex (RPA), a heterotrimer composed of RPA1, RPA2 and RPA3. Also component of the aRPA, the alternative replication protein A complex, a trimeric complex similar to the replication protein A complex/RPA but where RPA1 and RPA3 are associated with RPA4 instead of RPA2.

    Three dimensional structures from OCA and Proteopedia for RPA3 Gene

neXtProt entry for RPA3 Gene

Post-translational modifications for RPA3 Gene

  • Ubiquitinated by RFWD3 at stalled replication forks in response to DNA damage: ubiquitination by RFWD3 does not lead to degradation by the proteasome and promotes removal of the RPA complex from stalled replication forks, promoting homologous recombination (PubMed:26474068).
  • Ubiquitination at isoforms=23, isoforms=33, Lys39, and Lys88
  • Modification sites at PhosphoSitePlus

Other Protein References for RPA3 Gene

No data available for DME Specific Peptides for RPA3 Gene

Domains & Families for RPA3 Gene

Gene Families for RPA3 Gene

HGNC:
Human Protein Atlas (HPA):
  • Predicted intracellular proteins

Protein Domains for RPA3 Gene

Suggested Antigen Peptide Sequences for RPA3 Gene

Graphical View of Domain Structure for InterPro Entry

genes like me logo Genes that share domains with RPA3: view

No data available for UniProtKB/Swiss-Prot for RPA3 Gene

Function for RPA3 Gene

Molecular function for RPA3 Gene

GENATLAS Biochemistry:
replication protein A,subunit 3,component 14kDa of a trimeric single strand binding protein,involved in replication,homologous recombination and nucleotide excision repair,colocalizing with RAD51 on synapsed axes in meiosis and facilitating RAD51 function,also colocalizing with BLM in meiotic prophase of prophase nuclei of spermatocytes
UniProtKB/Swiss-Prot Function:
As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage (PubMed:9430682). In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response (PubMed:24332808). It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin, in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair (PubMed:7697716). Plays also a role in base excision repair (BER), probably through interaction with UNG (PubMed:9765279). Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA3 has its own single-stranded DNA-binding activity and may be responsible for polarity of the binding of the complex to DNA (PubMed:19010961). As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange (PubMed:19996105).

Phenotypes From GWAS Catalog for RPA3 Gene

Gene Ontology (GO) - Molecular Function for RPA3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003677 DNA binding IEA --
GO:0003684 damaged DNA binding IDA 7700386
GO:0003697 single-stranded DNA binding IDA 19010961
GO:0005515 protein binding IPI 2159011
genes like me logo Genes that share ontologies with RPA3: view
genes like me logo Genes that share phenotypes with RPA3: view

Animal Model Products

CRISPR Products

miRNA for RPA3 Gene

miRTarBase miRNAs that target RPA3

Inhibitory RNA Products

Clone Products

  • Applied Biological Materials Clones for RPA3
  • Vectors and viruses for ORF, Lenti, Retro, Adenovirus, AAV, and more

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for RPA3 Gene

Localization for RPA3 Gene

Subcellular locations from UniProtKB/Swiss-Prot for RPA3 Gene

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for RPA3 gene
Compartment Confidence
nucleus 5
cytoskeleton 2
cytosol 2
plasma membrane 1
mitochondrion 1

Gene Ontology (GO) - Cellular Components for RPA3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IEA --
GO:0005654 nucleoplasm TAS --
GO:0005662 DNA replication factor A complex IDA 11927569
genes like me logo Genes that share ontologies with RPA3: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for RPA3 Gene

Pathways & Interactions for RPA3 Gene

SuperPathway Contained pathways
1 Telomere C-strand (Lagging Strand) Synthesis
2 Translesion synthesis by Y family DNA polymerases bypasses lesions on DNA template
3 DNA Double-Strand Break Repair
4 Transcription-Coupled Nucleotide Excision Repair (TC-NER)
5 Mismatch repair
genes like me logo Genes that share pathways with RPA3: view

Gene Ontology (GO) - Biological Process for RPA3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000082 G1/S transition of mitotic cell cycle TAS --
GO:0000723 telomere maintenance TAS 24747047
GO:0000724 double-strand break repair via homologous recombination IMP 17765923
GO:0006260 DNA replication IEA,TAS --
GO:0006281 DNA repair IEA --
genes like me logo Genes that share ontologies with RPA3: view

No data available for SIGNOR curated interactions for RPA3 Gene

Drugs & Compounds for RPA3 Gene

(1) Drugs for RPA3 Gene - From: Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials

(2) Additional Compounds for RPA3 Gene - From: Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
genes like me logo Genes that share compounds with RPA3: view

Transcripts for RPA3 Gene

Unigene Clusters for RPA3 Gene

Replication protein A3, 14kDa:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

Clone Products

  • Applied Biological Materials Clones for RPA3
  • Vectors and viruses for ORF, Lenti, Retro, Adenovirus, AAV, and more

Alternative Splicing Database (ASD) splice patterns (SP) for RPA3 Gene

ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6 ^ 7a · 7b ^ 8a · 8b ^ 9a · 9b
SP1: - -
SP2: -
SP3:
SP4:

Relevant External Links for RPA3 Gene

GeneLoc Exon Structure for
RPA3
ECgene alternative splicing isoforms for
RPA3

Expression for RPA3 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for RPA3 Gene

Protein differential expression in normal tissues from HIPED for RPA3 Gene

This gene is overexpressed in NK cells (6.8) and Tlymphocyte (6.5).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for RPA3 Gene



Protein tissue co-expression partners for RPA3 Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of RPA3 Gene:

RPA3

SOURCE GeneReport for Unigene cluster for RPA3 Gene:

Hs.487540

Evidence on tissue expression from TISSUES for RPA3 Gene

  • Kidney(4.3)
  • Liver(4.2)
  • Lung(4.2)
  • Nervous system(3.9)
genes like me logo Genes that share expression patterns with RPA3: view

Primer Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for RPA3 Gene

Orthologs for RPA3 Gene

This gene was present in the common ancestor of chordates.

Orthologs for RPA3 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia RPA3 33 34
  • 99.17 (n)
dog
(Canis familiaris)
Mammalia RPA3 33 34
  • 91.46 (n)
cow
(Bos Taurus)
Mammalia RPA3 33 34
  • 89.44 (n)
rat
(Rattus norvegicus)
Mammalia Rpa3 33
  • 82.92 (n)
mouse
(Mus musculus)
Mammalia Rpa3 33 16 34
  • 82.37 (n)
oppossum
(Monodelphis domestica)
Mammalia RPA3 34
  • 66 (a)
OneToOne
chicken
(Gallus gallus)
Aves RPA3 33 34
  • 69.42 (n)
lizard
(Anolis carolinensis)
Reptilia RPA3 34
  • 61 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia rpa3 33
  • 69.54 (n)
Str.10202 33
African clawed frog
(Xenopus laevis)
Amphibia Xl.7376 33
zebrafish
(Danio rerio)
Actinopterygii rpa3 33 34
  • 62.39 (n)
sea squirt
(Ciona savignyi)
Ascidiacea CSA.7086 34
  • 31 (a)
OneToOne
Species where no ortholog for RPA3 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for RPA3 Gene

ENSEMBL:
Gene Tree for RPA3 (if available)
TreeFam:
Gene Tree for RPA3 (if available)

Paralogs for RPA3 Gene

Pseudogenes.org Pseudogenes for RPA3 Gene

genes like me logo Genes that share paralogs with RPA3: view

No data available for Paralogs for RPA3 Gene

Variants for RPA3 Gene

Sequence variations from dbSNP and Humsavar for RPA3 Gene

SNP ID Clin Chr 07 pos Sequence Context AA Info Type
rs1000060916 -- 7,669,131(+) ACTTG(G/T)GGGAA intron-variant
rs1000078765 -- 7,668,895(+) ACTCT(A/G)TTGCA intron-variant
rs1000091289 -- 7,650,205(+) ATTTA(C/T)GTTCT intron-variant
rs1000098337 -- 7,700,898(+) AAAAA(A/C)AAAAT intron-variant
rs1000162636 -- 7,689,409(+) CATTG(G/T)TGAAC intron-variant

Structural Variations from Database of Genomic Variants (DGV) for RPA3 Gene

Variant ID Type Subtype PubMed ID
dgv6249n100 CNV gain 25217958
esv1554962 CNV insertion 17803354
esv1736638 CNV insertion 17803354
esv2733939 CNV deletion 23290073
esv2752174 CNV gain 17911159
esv2752177 CNV gain 17911159
esv2761144 CNV gain+loss 21179565
esv3891035 CNV gain 25118596
esv3891036 CNV gain 25118596
nsv1034199 CNV loss 25217958
nsv1073637 CNV deletion 25765185
nsv606042 CNV gain 21841781
nsv606044 CNV gain 21841781
nsv606045 CNV loss 21841781
nsv8043 CNV gain 18304495

Variation tolerance for RPA3 Gene

Residual Variation Intolerance Score: 33.2% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.04; 1.02% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for RPA3 Gene

Human Gene Mutation Database (HGMD)
RPA3
SNPedia medical, phenotypic, and genealogical associations of SNPs for
RPA3

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for RPA3 Gene

Disorders for RPA3 Gene

MalaCards: The human disease database

(4) MalaCards diseases for RPA3 Gene - From: HGMD and DISEASES

Disorder Aliases PubMed IDs
epidemic typhus
  • sylvatic typhus
ovary serous adenocarcinoma
  • malignant ovarian serous tumor
retinitis pigmentosa-12, autosomal recessive
  • retinitis pigmentosa 12
ovarian cystadenocarcinoma
  • cystadenocarcinoma of ovary
- elite association - COSMIC cancer census association via MalaCards
Search RPA3 in MalaCards View complete list of genes associated with diseases

Relevant External Links for RPA3

Genetic Association Database (GAD)
RPA3
Human Genome Epidemiology (HuGE) Navigator
RPA3
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
RPA3
genes like me logo Genes that share disorders with RPA3: view

No data available for UniProtKB/Swiss-Prot and Genatlas for RPA3 Gene

Publications for RPA3 Gene

  1. Cloning, overexpression, and genomic mapping of the 14-kDa subunit of human replication protein A. (PMID: 8454588) Umbricht CB … Kelly TJ (The Journal of biological chemistry 1993) 2 3 4 60
  2. RFWD3-Dependent Ubiquitination of RPA Regulates Repair at Stalled Replication Forks. (PMID: 26474068) Elia AE … Elledge SJ (Molecular cell 2015) 3 4 60
  3. PRP19 transforms into a sensor of RPA-ssDNA after DNA damage and drives ATR activation via a ubiquitin-mediated circuitry. (PMID: 24332808) Maréchal A … Zou L (Molecular cell 2014) 3 4 60
  4. Comprehensive screen of genetic variation in DNA repair pathway genes and postmenopausal breast cancer risk. (PMID: 20496165) Monsees GM … Han J (Breast cancer research and treatment 2011) 3 45 60
  5. Human variation in alcohol response is influenced by variation in neuronal signaling genes. (PMID: 20201926) Joslyn G … White RL (Alcoholism, clinical and experimental research 2010) 3 45 60

Products for RPA3 Gene

Sources for RPA3 Gene

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