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Aliases for RPA3 Gene

Aliases for RPA3 Gene

  • Replication Protein A3, 14kDa 2 3
  • Replication Factor A Protein 3 3 4
  • RF-A Protein 3 3 4
  • RP-A P14 3 4
  • REPA3 3 4
  • Replication Protein A3 (14kD) 2
  • RPA14 4

External Ids for RPA3 Gene

Previous GeneCards Identifiers for RPA3 Gene

  • GC07M007320
  • GC07M007420
  • GC07M007449

Summaries for RPA3 Gene

GeneCards Summary for RPA3 Gene

RPA3 (Replication Protein A3, 14kDa) is a Protein Coding gene. Diseases associated with RPA3 include epidemic typhus and ovary serous adenocarcinoma. Among its related pathways are Cell Cycle, Mitotic and Cellular Senescence. GO annotations related to this gene include single-stranded DNA binding and damaged DNA binding.

UniProtKB/Swiss-Prot for RPA3 Gene

  • As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage (PubMed:9430682). In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response (PubMed:24332808). It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin, in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair (PubMed:7697716). Plays also a role in base excision repair (BER), probably through interaction with UNG (PubMed:9765279). Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA3 has its own single-stranded DNA-binding activity and may be responsible for polarity of the binding of the complex to DNA (PubMed:19010961). As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange (PubMed:19996105).

Gene Wiki entry for RPA3 Gene

No data available for Entrez Gene Summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for RPA3 Gene

Genomics for RPA3 Gene

Regulatory Elements for RPA3 Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for RPA3 Gene

Chromosome:
7
Start:
7,636,518 bp from pter
End:
7,718,607 bp from pter
Size:
82,090 bases
Orientation:
Minus strand

Genomic View for RPA3 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
GeneLoc Logo Gene Density

RefSeq DNA sequence for RPA3 Gene

Proteins for RPA3 Gene

  • Protein details for RPA3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P35244-RFA3_HUMAN
    Recommended name:
    Replication protein A 14 kDa subunit
    Protein Accession:
    P35244
    Secondary Accessions:
    • Q549U6

    Protein attributes for RPA3 Gene

    Size:
    121 amino acids
    Molecular mass:
    13569 Da
    Quaternary structure:
    • Component of the canonical replication protein A complex (RPA), an heterotrimer composed of RPA1, RPA2 and RPA3. Also component of the aRPA, the alternative replication protein A complex, a trimeric complex similar to the replication protein A complex/RPA but where RPA1 and RPA3 are associated with RPA4 instead of RPA2.

    Three dimensional structures from OCA and Proteopedia for RPA3 Gene

neXtProt entry for RPA3 Gene

Proteomics data for RPA3 Gene at MOPED

Post-translational modifications for RPA3 Gene

  • Ubiquitination at Lys 23, Lys 33, Lys 39, and Lys 88
  • Modification sites at PhosphoSitePlus

Other Protein References for RPA3 Gene

No data available for DME Specific Peptides for RPA3 Gene

Domains & Families for RPA3 Gene

Protein Domains for RPA3 Gene

Suggested Antigen Peptide Sequences for RPA3 Gene

Graphical View of Domain Structure for InterPro Entry

genes like me logo Genes that share domains with RPA3: view

No data available for Gene Families and UniProtKB/Swiss-Prot for RPA3 Gene

Function for RPA3 Gene

Molecular function for RPA3 Gene

GENATLAS Biochemistry:
replication protein A,subunit 3,component 14kDa of a trimeric single strand binding protein,involved in replication,homologous recombination and nucleotide excision repair,colocalizing with RAD51 on synapsed axes in meiosis and facilitating RAD51 function,also colocalizing with BLM in meiotic prophase of prophase nuclei of spermatocytes
UniProtKB/Swiss-Prot Function:
As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage (PubMed:9430682). In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response (PubMed:24332808). It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin, in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair (PubMed:7697716). Plays also a role in base excision repair (BER), probably through interaction with UNG (PubMed:9765279). Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA3 has its own single-stranded DNA-binding activity and may be responsible for polarity of the binding of the complex to DNA (PubMed:19010961). As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange (PubMed:19996105).

Gene Ontology (GO) - Molecular Function for RPA3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003677 DNA binding --
GO:0003684 damaged DNA binding IDA 7700386
GO:0003697 single-stranded DNA binding IDA 19010961
GO:0005515 protein binding IPI 2159011
genes like me logo Genes that share ontologies with RPA3: view
genes like me logo Genes that share phenotypes with RPA3: view

miRNA for RPA3 Gene

miRTarBase miRNAs that target RPA3

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for RPA3

In Situ Assay Products

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , Animal Models , Transcription Factor Targets and HOMER Transcription for RPA3 Gene

Localization for RPA3 Gene

Subcellular locations from UniProtKB/Swiss-Prot for RPA3 Gene

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for RPA3 Gene COMPARTMENTS Subcellular localization image for RPA3 gene
Compartment Confidence
nucleus 5
extracellular 1

Gene Ontology (GO) - Cellular Components for RPA3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus --
GO:0005654 nucleoplasm TAS --
GO:0005662 DNA replication factor A complex IDA 11927569
genes like me logo Genes that share ontologies with RPA3: view

Pathways & Interactions for RPA3 Gene

SuperPathways for RPA3 Gene

Superpath Contained pathways
1 Lagging Strand Synthesis
2 Translesion synthesis by Y family DNA polymerases bypasses lesions on DNA template
3 Transcription-Coupled Nucleotide Excision Repair (TC-NER)
4 DNA Double-Strand Break Repair
5 CDK-mediated phosphorylation and removal of Cdc6
.78
genes like me logo Genes that share pathways with RPA3: view

PCR Array Products

  • Pathway & Disease-focused RT² Profiler PCR Arrays

Gene Ontology (GO) - Biological Process for RPA3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000082 G1/S transition of mitotic cell cycle TAS --
GO:0000278 mitotic cell cycle TAS --
GO:0000718 nucleotide-excision repair, DNA damage removal --
GO:0000722 telomere maintenance via recombination TAS --
GO:0000723 telomere maintenance TAS --
genes like me logo Genes that share ontologies with RPA3: view

No data available for SIGNOR curated interactions for RPA3 Gene

Drugs & Compounds for RPA3 Gene

(1) Drugs for RPA3 Gene - From: NovoSeek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Zinc Approved Pharma 1404

(2) Additional Compounds for RPA3 Gene - From: NovoSeek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
NaCl
RPA 2
genes like me logo Genes that share compounds with RPA3: view

Transcripts for RPA3 Gene

Unigene Clusters for RPA3 Gene

Replication protein A3, 14kDa:
Representative Sequences:

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for RPA3

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for RPA3 Gene

ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6 ^ 7a · 7b ^ 8a · 8b ^ 9a · 9b
SP1: - -
SP2: -
SP3:
SP4:

Relevant External Links for RPA3 Gene

GeneLoc Exon Structure for
RPA3
ECgene alternative splicing isoforms for
RPA3

Expression for RPA3 Gene

mRNA expression in normal human tissues for RPA3 Gene

Protein differential expression in normal tissues from HIPED for RPA3 Gene

This gene is overexpressed in NK cells (6.8) and Tlymphocyte (6.5).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for RPA3 Gene



SOURCE GeneReport for Unigene cluster for RPA3 Gene Hs.487540

genes like me logo Genes that share expression patterns with RPA3: view

Protein tissue co-expression partners for RPA3 Gene

- Elite partner

Primer Products

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues and mRNA Expression by UniProt/SwissProt for RPA3 Gene

Orthologs for RPA3 Gene

This gene was present in the common ancestor of chordates.

Orthologs for RPA3 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia RPA3 35
  • 89.44 (n)
  • 86.67 (a)
RPA3 36
  • 86 (a)
OneToOne
dog
(Canis familiaris)
Mammalia RPA3 35
  • 91.46 (n)
  • 89.26 (a)
RPA3 36
  • 88 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Rpa3 35
  • 82.37 (n)
  • 76.86 (a)
Rpa3 16
Rpa3 36
  • 77 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia RPA3 35
  • 99.17 (n)
  • 98.35 (a)
RPA3 36
  • 98 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Rpa3 35
  • 82.92 (n)
  • 79.34 (a)
oppossum
(Monodelphis domestica)
Mammalia RPA3 36
  • 66 (a)
OneToOne
chicken
(Gallus gallus)
Aves RPA3 35
  • 69.42 (n)
  • 66.94 (a)
RPA3 36
  • 67 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia RPA3 36
  • 61 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia rpa3 35
  • 69.54 (n)
  • 62.93 (a)
Str.10202 35
African clawed frog
(Xenopus laevis)
Amphibia Xl.7376 35
zebrafish
(Danio rerio)
Actinopterygii rpa3 35
  • 62.39 (n)
  • 57.26 (a)
rpa3 36
  • 57 (a)
OneToOne
sea squirt
(Ciona savignyi)
Ascidiacea CSA.7086 36
  • 31 (a)
OneToOne
Species with no ortholog for RPA3:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for RPA3 Gene

ENSEMBL:
Gene Tree for RPA3 (if available)
TreeFam:
Gene Tree for RPA3 (if available)

Paralogs for RPA3 Gene

Pseudogenes.org Pseudogenes for RPA3 Gene

genes like me logo Genes that share paralogs with RPA3: view

No data available for Paralogs for RPA3 Gene

Variants for RPA3 Gene

Sequence variations from dbSNP and Humsavar for RPA3 Gene

SNP ID Clin Chr 07 pos Sequence Context AA Info Type MAF
rs12120 -- 7,636,771(-) ATAAA(G/T)CAGTT downstream-variant-500B
rs6463709 -- 7,638,126(+) GTTAA(C/T)AAATG intron-variant
rs6463710 -- 7,638,178(+) TTTAG(C/T)AGTAG intron-variant
rs10265415 -- 7,637,113(+) TTAAT(C/G)TACAA intron-variant
rs10280185 -- 7,636,550(+) GGGTA(C/T)CAAGG downstream-variant-500B

Structural Variations from Database of Genomic Variants (DGV) for RPA3 Gene

Variant ID Type Subtype PubMed ID
esv2752174 CNV Gain 17911159
dgv7158n71 CNV Gain 21882294
dgv7159n71 CNV Gain 21882294
nsv887477 CNV Gain 21882294
nsv8043 CNV Gain 18304495
dgv7160n71 CNV Gain 21882294
dgv7161n71 CNV Loss 21882294
nsv887483 CNV Gain 21882294
nsv887484 CNV Gain 21882294
nsv887486 CNV Loss 21882294
esv2733939 CNV Deletion 23290073
esv1736638 CNV Insertion 17803354
esv1554962 CNV Insertion 17803354
esv2752177 CNV Gain 17911159
nsv887487 CNV Loss 21882294

Variation tolerance for RPA3 Gene

Residual Variation Intolerance Score: 33.23% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.04; 1.02% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for RPA3 Gene

HapMap Linkage Disequilibrium report
RPA3
Human Gene Mutation Database (HGMD)
RPA3

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for RPA3 Gene

Disorders for RPA3 Gene

MalaCards: The human disease database

(3) MalaCards diseases for RPA3 Gene - From: DISEASES

Disorder Aliases PubMed IDs
epidemic typhus
  • sylvatic typhus
ovary serous adenocarcinoma
  • malignant ovarian serous tumor
ovarian cystadenocarcinoma
  • cystadenocarcinoma of the ovary
- elite association
Search RPA3 in MalaCards View complete list of genes associated with diseases

Relevant External Links for RPA3

Genetic Association Database (GAD)
RPA3
Human Genome Epidemiology (HuGE) Navigator
RPA3
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
RPA3
genes like me logo Genes that share disorders with RPA3: view

No data available for UniProtKB/Swiss-Prot and Genatlas for RPA3 Gene

Publications for RPA3 Gene

  1. Replication protein A (RPA1a) is required for meiotic and somatic DNA repair but is dispensable for DNA replication and homologous recombination in rice. (PMID: 19812186) Chang Y. … Wu C. (Plant Physiol. 2009) 23 67
  2. Structure of the full-length human RPA14/32 complex gives insights into the mechanism of DNA binding and complex formation. (PMID: 17976647) Deng X. … Borgstahl G.E. (J. Mol. Biol. 2007) 23 67
  3. A higher plant has three different types of RPA heterotrimeric complex. (PMID: 16428324) Ishibashi T. … Sakaguchi K. (J. Biochem. 2006) 23 67
  4. Phosphorylation of replication protein A by S-phase checkpoint kinases. (PMID: 16412704) Liu J.S. … Melendy T. (DNA Repair (Amst.) 2006) 23 67
  5. Interaction and colocalization of Rad9/Rad1/Hus1 checkpoint complex with replication protein A in human cells. (PMID: 15897895) Wu X. … Zou Y. (Oncogene 2005) 23 67

Products for RPA3 Gene

Sources for RPA3 Gene

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