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RPA2 Gene

protein-coding   GIFtS: 72
GCID: GC01M028218

Replication Protein A2, 32kDa

(Previous name: replication protein A2 (32kD))
  See RPA2-related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Replication Protein A2, 32kDa1 2     RP-A P322 3
Replication Factor A Protein 22 3     RP-A P342 3
Replication Protein A 34 KDa Subunit2 3     Replication Protein A2 (32kD)1
REPA22 3     Replication Protein A 32 KDa Subunit2
RPA322 3     RPA343
RF-A Protein 22 3     

External Ids:    HGNC: 102901   Entrez Gene: 61182   Ensembl: ENSG000001177487   OMIM: 1798365   UniProtKB: P159273   

Export aliases for RPA2 gene to outside databases

Previous GC identifers: GC01P027225 GC01M027451 GC01M027822 GC01M027902 GC01M028090 GC01M026473


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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GeneCards Summary for RPA2 Gene:
RPA2 (replication protein A2, 32kDa) is a protein-coding gene. Diseases associated with RPA2 include xeroderma pigmentosum, group a, and familial melanoma. GO annotations related to this gene include protein phosphatase binding and protein N-terminus binding. An important paralog of this gene is RPA4.

UniProtKB/Swiss-Prot: RFA2_HUMAN, P15927
Function: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by
single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA
double-strand break repair factor RAD51 to chromatin in response to DNA damage
Function: Functions as component of the alternative replication protein A complex (aRPA). aRPA binds
single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and
cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha
but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual
incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange

Gene Wiki entry for RPA2 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000001.10  NT_032977.10  NC_018912.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the RPA2 gene promoter:
         HEN1   PPAR-alpha   CREB   deltaCREB   AP-4   MRF-2   C/EBPalpha   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidRPA2 promoter sequence
   Search Chromatin IP Primers for RPA2

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat RPA2


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 1p35   Ensembl cytogenetic band:  1p35.3   HGNC cytogenetic band: 1p35

RPA2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
RPA2 gene location

GeneLoc information about chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01M028218:  view genomic region     (about GC identifiers)

Start:
28,218,035 bp from pter      End:
28,241,257 bp from pter
Size:
23,223 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., eBioscience, and/or antibodies-online,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., eBioscience, and/or antibodies-online, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, Cloud-Clone Corp, antibodies-online, and/or others.)
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UniProtKB/Swiss-Prot: RFA2_HUMAN, P15927 (See protein sequence)
Recommended Name: Replication protein A 32 kDa subunit  
Size: 270 amino acids; 29247 Da
Subunit: Heterotrimer of 70, 32 and 14 kDa chains (canonical replication protein A complex). Component of the
alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. The DNA-binding activity may
reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN. Directly interacts with
PPP4R2, but not with SMEK2; this interaction is DNA damage-dependent and leads RPA2 dephosphorylation by PPP4C
recruitment. Interacts with RAD51, preferentially when hyperphosphorylated. Directly interacts with RFWD3
Selected PDB 3D structures from and Proteopedia for RPA2 (see all 8):
1DPU (3D)        1L1O (3D)        1QUQ (3D)        1Z1D (3D)        2PI2 (3D)        2PQA (3D)    
Secondary accessions: Q52II0 Q5TEI9 Q5TEJ5
Alternative splicing: 3 isoforms:  P15927-1   P15927-2   P15927-3   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for RPA2: NX_P15927

Explore proteomics data for RPA2 at MOPED

Post-translational modifications: 

  • Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). In response to DNA damage,
    recruited to DNA-repair nuclear foci, as a hypophosphorylated form. The necessary dephosphorylation step is
    catalyzed by PP4. Subsequent hyperphosphorylation, catalyzed by ATR, is required for RAD51 recruitment to
    chromatin and efficient DNA repair. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA,
    and by CDK1. Phosphorylation at Thr-21 depends upon RFWD3 presence1
  • Ubiquitination2 at Lys127
  • Modification sites at PhosphoSitePlus

  • See RPA2 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (2 alternative transcripts): 
    NP_001273005.1  NP_002937.1  

    ENSEMBL proteins: 
     ENSP00000363021   ENSP00000413541   ENSP00000363017   ENSP00000363015   ENSP00000387649  
    Reactome Protein details: P15927

    RPA2 Human Recombinant Protein Products:

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    antibodies-online proteins for RPA2 (10 products) 

     
    antibodies-online peptides for RPA2

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    antibodies-online antibodies for RPA2 (131 products) 

    RPA2 Assay Products:

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    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    5 InterPro protein domains:
     IPR011991 WHTH_DNA-bd_dom
     IPR012340 NA-bd_OB-fold
     IPR014646 RPA32
     IPR004365 NA-bd_OB_tRNA
     IPR014892 RPA_C

    Graphical View of Domain Structure for InterPro Entry P15927

    ProtoNet protein and cluster: P15927

    1 Blocks protein domain: IPB004365 OB-fold nucleic acid binding domain


    Find genes that share domains with RPA2           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: RFA2_HUMAN, P15927
    Function: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by
    single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA
    double-strand break repair factor RAD51 to chromatin in response to DNA damage
    Function: Functions as component of the alternative replication protein A complex (aRPA). aRPA binds
    single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and
    cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha
    but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual
    incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange

         Genatlas biochemistry entry for RPA2:
    replication protein A,subunit 2,component of a trimeric single strand binding protein,involved in
    replication,homologous recombination and nucleotide excision repair,colocolizing with RAD51 on synapsed axes in
    meiosis and facilitating RAD51 function,also colocalizing with BLM in meiotic prophase of prophase nuclei of
    spermatocytes

         Gene Ontology (GO): Selected molecular function terms (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003676nucleic acid binding ----
    GO:0003677DNA binding ----
    GO:0003697single-stranded DNA binding IEA--
    GO:0005515protein binding IPI10449415
    GO:0019903protein phosphatase binding IPI--
         
    Find genes that share ontologies with RPA2           About GenesLikeMe


    Phenotypes:
         7 GenomeRNAi human phenotypes for RPA2:
     Bright nuclei  Decreased viability  Decreased viability of wild-ty  Increased S DNA content, incre 
     Increased gamma-H2AX phosphory  S arrest  Synthetic lethal with Ras 

    Animal Models:
       genOway: Develop your customized and physiologically relevant rodent model for RPA2

    miRNA
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    miRTarBase miRNAs that target RPA2:
    hsa-mir-19b-3p (MIRT031089), hsa-mir-130b-3p (MIRT020141), hsa-mir-93-5p (MIRT027975), hsa-mir-1226-3p (MIRT036453), hsa-mir-20a-5p (MIRT050504), hsa-mir-92a-3p (MIRT049074)

    Block miRNA regulation of human, mouse, rat RPA2 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate RPA2 (see all 22):
    hsa-miR-106a hsa-let-7a-2* hsa-miR-301a hsa-miR-519a hsa-miR-93 hsa-let-7g* hsa-miR-526b* hsa-miR-17
    SwitchGear 3'UTR luciferase reporter plasmidRPA2 3' UTR sequence
    Inhib. RNA
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    Addgene plasmids for RPA2 

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    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    RFA2_HUMAN, P15927: Nucleus. Nucleus, PML body. Note=Also present in PML nuclear bodies. Redistributes to
    discrete nuclear foci upon DNA damage
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus5
    cytosol2

    Gene Ontology (GO): 5 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IDA--
    GO:0005654nucleoplasm TAS--
    GO:0005662DNA replication factor A complex IPI16135809
    GO:0005730NOT nucleolus IDA--
    GO:0016605PML body IDA12814551

    Find genes that share ontologies with RPA2           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for RPA2 About   (see all 18)  
    See pathways by source

    SuperPathSelected contained pathways About (see all per SuperPath)
    1Telomere C-strand (Lagging Strand) Synthesis
    Gap-filling DNA repair synthesis and ligation in TC-NER0.94
    Removal of the Flap Intermediate from the C-strand0.00
    Repair synthesis of patch ~27-30 bases long by DNA polymerase0.94
    Lagging Strand Synthesis0.00
    Telomere C-strand (Lagging Strand) Synthesis0.79
    Removal of the Flap Intermediate0.00
    Extension of Telomeres0.79
    Processive synthesis on the C-strand of the telomere0.00
    2Global Genomic NER (GG-NER)
    Global Genomic NER (GG-NER)0.69
    Formation of incision complex in GG-NER0.62
    Nucleotide excision repair0.69
    Nucleotide Excision Repair Pathway0.48
    Dual incision reaction in GG-NER0.62
    3Activation of the pre-replicative complex
    Activation of the pre-replicative complex0.66
    Cell cycle Start of DNA replication in early S phase0.53
    DNA Replication0.66
    4E2F mediated regulation of DNA replication
    G1/S Transition0.81
    G1 to S cell cycle control0.35
    Mitotic G1-G1/S phases0.81
    5DNA strand elongation
    DNA strand elongation0.63
    DNA replication0.63


    Find genes that share SuperPaths with RPA2           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    3 Downloadable PowerPoint Slides of GeneGlobe Pathway Central Maps for RPA2
        Mismatch Repair in Eukaryotes
    DNA Repair Mechanisms
    Nucleotide Excision Repair Pathway

    2 Cell Signaling Technology (CST) Pathways for RPA2
        Cell Cycle / Checkpoint Control
    DNA Damage

    1 GeneGo (Thomson Reuters) Pathway for RPA2
        Cell cycle Start of DNA replication in early S phase

    4 BioSystems Pathways for RPA2
        DNA damage response
    G1 to S cell cycle control
    DNA Replication
    RB in Cancer


    Selected Reactome Pathways for RPA2 (see all 14)
        Regulation of HSF1-mediated heat shock response
    Repair synthesis for gap-filling by DNA polymerase in TC-NER
    Activation of ATR in response to replication stress
    Removal of the Flap Intermediate
    Processing of DNA double-strand break ends


    5 Kegg Pathways  (Kegg details for RPA2):
        DNA replication
    Nucleotide excision repair
    Mismatch repair
    Homologous recombination
    Fanconi anemia pathway

        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for RPA2
    Interactions:

        GeneGlobe Interaction Network for RPA2

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for RPA2 (P159271, 2, 3 ENSP000003630214) via UniProtKB, MINT, STRING, and/or I2D (see all 186)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    RPA1P276941, 3, ENSP000002547194EBI-621404,EBI-621389 I2D: score=5 STRING: ENSP00000254719
    RPA3P352441, 3, ENSP000002231294EBI-621404,EBI-621428 I2D: score=5 STRING: ENSP00000223129
    CHEK1O147572, 3, ENSP000002789164MINT-8046309 I2D: score=1 STRING: ENSP00000278916
    GAPDHP044061, 3EBI-621404,EBI-354056 I2D: score=4 
    WRNQ141912, 3, ENSP000002981394MINT-8048594 MINT-8048571 I2D: score=1 STRING: ENSP00000298139
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 17):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000082G1/S transition of mitotic cell cycle TAS--
    GO:0000278mitotic cell cycle TAS--
    GO:0000718nucleotide-excision repair, DNA damage removal TAS--
    GO:0000722telomere maintenance via recombination TAS--
    GO:0000723telomere maintenance TAS--

    Find genes that share ontologies with RPA2           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for RPA2 (RFA2)

    Selected Novoseek inferred chemical compound relationships for RPA2 gene (see all 17)    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    rpa 2 98.3 167 11731442 (9), 20130019 (8), 16483312 (8), 19671522 (7) (see all 39)
    adozelesin 73.6 9 12533671 (4), 14643438 (2)
    camptothecin 72.1 30 16483312 (7), 10064605 (4), 15856006 (3), 15060176 (2) (see all 6)
    aphidicolin 69.7 9 14643438 (3), 15661754 (2), 12533671 (1), 15938632 (1)
    7-hydroxystaurosporine 65.2 1 10064605 (1)
    hydroxyurea 54.9 23 14643438 (3), 20130019 (3), 19704001 (2), 16483312 (2) (see all 5)
    etoposide 31.2 2 10064605 (1), 19787780 (1)
    threonine 19 1 19704001 (1)
    wortmannin 15.9 1 11731442 (1)
    caffeine 15.3 2 15060176 (1), 11731442 (1)



    Find genes that share compounds with RPA2           About GenesLikeMe



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for RPA2 gene (2 alternative transcripts): 
    NM_001286076.1  NM_002946.4  

    Unigene Cluster for RPA2:

    Replication protein A2, 32kDa
    Hs.79411  [show with all ESTs]
    Unigene Representative Sequence: NM_002946
    5 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000373912(uc001bpe.1 uc010ofp.1) ENST00000419958 ENST00000373909
    ENST00000313433 ENST00000444045
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    hsa-miR-106a hsa-let-7a-2* hsa-miR-301a hsa-miR-519a hsa-miR-93 hsa-let-7g* hsa-miR-526b* hsa-miR-17
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    Additional mRNA sequence: 

    AK298759.1 AK300691.1 AK312956.1 BC001630.1 BC012157.1 BC021257.2 CR450348.1 J05249.1 

    14 DOTS entries:

    DT.418275  DT.91764464  DT.121459182  DT.91764463  DT.121459150  DT.121459217  DT.95201704  DT.97859731 
    DT.95201728  DT.121459157  DT.121459229  DT.91764450  DT.95201706  DT.95201730 

    Selected AceView cDNA sequences (see all 382):

    CR594823 BX442975 BI020968 BQ668543 AI990805 AA182454 CK825043 BP369294 
    CR590863 BM839986 BQ064852 BQ059808 BE297131 BC001630 BQ673815 BQ641985 
    BQ083162 BM698831 BM669612 BI261314 BI519710 AA226830 R95969 AI870836 

    GeneLoc Exon Structure

    3 Alternative Splicing Database (ASD) splice patterns (SP) for RPA2    About this scheme

    ExUns: 1a · 1b · 1c ^ 2a · 2b ^ 3 ^ 4 ^ 5 ^ 6 ^ 7a · 7b ^ 8 ^ 9
    SP1:              -     -                                                         
    SP2:                                                                              
    SP3:                    -                                                         


    ECgene alternative splicing isoforms for RPA2

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

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    RPA2 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: AAGTTCTGCG
    RPA2 Expression
    About this image


    RPA2 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 7) fully expand
     
     Brain (Nervous System)    fully expand to see all 4 entries
             Cerebral Cortex
     
     Ovary (Reproductive System)    fully expand to see all 2 entries
             Ovarian Mesenchymal Stroma Cells Ovary Interstitium
             Oviduct
     
     Testis (Reproductive System)
             Leydig Cells Testis Interstitium
     
     Trophoblast (Extraembryonic Tissues)
             Trophoblast Cells Trophoblast
     
     Pancreas (Endocrine System)
             Islets of Langerhans
    RPA2 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    RPA2 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.79411
        Custom PCR Arrays for RPA2
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of eukaryotes.

    Orthologs for RPA2 gene from Selected species (see all 23)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Rpa21 , 5 replication protein A21, 5 85.56(n)1
    88.52(a)1
      4 (65.88 cM)5
    198911  NM_011284.31  NP_035414.31 
     1327683325 
    chicken
    (Gallus gallus)
    Aves RPA21 replication protein A2, 32kDa 67.6(n)
    71.49(a)
      419566  NM_001030892.1  NP_001026063.1 
    lizard
    (Anolis carolinensis)
    Reptilia --
    Uncharacterized protein
    63(a)
    1 → many
    GL343743.1(52034-61000)
    African clawed frog
    (Xenopus laevis)
    Amphibia Xl.56292 Xenopus laevis transcribed sequence with weak similarity more 77.92(n)    CA972904.1 
    zebrafish
    (Danio rerio)
    Actinopterygii rpa22 replication protein A2 75.54(n)   65226  AY007304.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta RPA21 Replication protein A2 43.93(n)
    33.05(a)
      35364  NM_136233.3  NP_610077.1 
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes RFA2(YNL312W)4
    RFA21
    Subunit of heterotrimeric Replication Protein A (RPA), more4
    RFA21
    42.25(n)1
    32.89(a)1
      14(48287-49216)4
    8554041, 4  NP_014087.11, 4 
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons RPA21 RPA2 45.47(n)
    35.09(a)
      816985  NM_128010.2  NP_565571.1 
    rice
    (Oryza sativa)
    Liliopsida Os02g08291001 Os02g0829100 43.85(n)
    32.37(a)
      4331236  NM_001055139.1  NP_001048604.1 


    ENSEMBL Gene Tree for RPA2 (if available)
    TreeFam Gene Tree for RPA2 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for RPA2 gene
    RPA42  
    1 SIMAP similar gene for RPA2 using alignment to 3 protein entries:     RFA2_HUMAN (see all proteins):
    RPA4

    Find genes that share paralogs with RPA2           About GenesLikeMe


    3 Pseudogenes.org Pseudogenes for RPA2
    PGOHUM00000238566 PGOHUM00000242874 PGOHUM00000248155


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for RPA2 (see all 645)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 1 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1809349341,2
    --28217607(+) TTCAGA/GAGTTG 1 -- int10--------
    rs171628681,2
    C,F,H--28217614(+) GTTGTC/GAAAAA 1 -- int121Minor allele frequency- N:0.00NA NS EA 1490
    rs1843085441,2
    --28217741(+) TTACAC/TGATCA 1 -- int10--------
    rs118006031,2
    C,F,H--28217914(+) TGACAT/CAGCCT 1 -- int111Minor allele frequency- C:0.01NS EA NA WA 702
    rs1887268171,2
    --28218050(+) TTACTA/GCAATA 1 -- ut310--------
    rs289049101,2
    C,F--28218088(-) ACAAAA/GTAAAT 1 -- ut315Minor allele frequency- G:0.01NS 190
    rs73561,2
    C,F,A,H--28218100(-) ACTGCA/GTTTTG 1 -- ut31 ese337Minor allele frequency- G:0.48MN EA NA NS WA CSA 4521
    rs1486477061,2
    --28218101(+) AAAATA/GCAGTA 1 -- ut310--------
    rs1179530421,2
    --28218246(+) CAAAGT/GGGGAA 1 -- ut311Minor allele frequency- G:0.01EA 120
    rs289049091,2
    C,F,H--28218312(-) AGCAGG/AACCAG 1 -- ut31 ese37Minor allele frequency- A:0.00NS NA 416

    HapMap Linkage Disequilibrium report for RPA2 (28218035 - 28241257 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 4 variations for RPA2:    About this table    
    Variant IDTypeSubtypePubMed ID
    nsv509069CNV Insertion20534489
    nsv871458CNV Gain21882294
    nsv871591CNV Gain21882294
    nsv870553CNV Gain21882294

    Human Gene Mutation Database (HGMD): RPA2
    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing RPA2
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 179836    OMIM disorders: --

    4 diseases for RPA2:    
    About MalaCards
    xeroderma pigmentosum, group a    familial melanoma    ataxia telangiectasia    xeroderma pigmentosum

    1 disease from the University of Copenhagen DISEASES database for RPA2:
    Ataxia telangiectasia

    Find genes that share disorders with RPA2           About GenesLikeMe

    9 Novoseek inferred disease relationships for RPA2 gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    ataxia telangiectasia 77.2 8 16483312 (2), 14872059 (1), 19339345 (1), 9270001 (1) (see all 5)
    xeroderma pigmentosum 42.2 1 10340474 (1)
    promyelocytic leukemia 29.6 1 12814551 (1)
    colon cancer 0 3 17361204 (3)
    breast cancer 0 1 11895905 (1)
    metastasis 0 2 17361204 (1)
    tumors 0 8 11895905 (2), 17970065 (1), 17361204 (1)
    carcinoma 0 3 11895905 (1), 17361204 (1)
    cancer 0 2 15856006 (1), 11895905 (1)

    Genetic Association Database (GAD): RPA2
    Human Genome Epidemiology (HuGE) Navigator: RPA2 (6 documents)

    Export disorders for RPA2 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for RPA2 gene, integrated from 10 sources (see all 204):
    (articles sorted by number of sources associating them with RPA2)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. A PP4 phosphatase complex dephosphorylates RPA2 to facilitate DNA repair via homologous recombination. (PubMed id 20154705)1, 2, 9 Lee D.H.... Chowdhury D. (Nat. Struct. Mol. Biol. 2010)
    2. ATR kinase activity regulates the intranuclear translocation of ATR and RPA following ionizing radiation. (PubMed id 12814551)1, 2, 9 Barr S.M.... Cimprich K.A. (Curr. Biol. 2003)
    3. RBT1, a novel transcriptional co-activator, binds the second subunit of replication protein A. (PubMed id 10982866)1, 2, 9 Cho J.M....Alaoui-Jamali M.A. (Nucleic Acids Res. 2000)
    4. Structure of the RPA trimerization core and its role in the multistep DNA-binding mechanism of RPA. (PubMed id 11927569)1, 2, 9 Bochkareva E.... Bochkarev A. (EMBO J. 2002)
    5. E3 ligase RFWD3 participates in replication checkpoint control. (PubMed id 21504906)1, 2 Gong Z. and Chen J. (J. Biol. Chem. 2011)
    6. Comprehensive screen of genetic variation in DNA repair pathway genes and postmenopausal breast cancer risk. (PubMed id 20496165)1, 4 Monsees G.M....Han J. (Breast Cancer Res. Treat. 2011)
    7. RING finger and WD repeat domain 3 (RFWD3) associates with replication protein A (RPA) and facilitates RPA-mediated DNA damage response. (PubMed id 21558276)1, 2 Liu S.... Wang Y. (J. Biol. Chem. 2011)
    8. Human variation in alcohol response is influenced by variation in neuronal signaling genes. (PubMed id 20201926)1, 4 Joslyn G....White R.L. (Alcohol. Clin. Exp. Res. 2010)
    9. Variation within DNA repair pathway genes and risk of multiple sclerosis. (PubMed id 20522537)1, 4 Briggs F.B....Barcellos L.F. (Am. J. Epidemiol. 2010)
    10. Genetic polymorphisms in 85 DNA repair genes and bladder cancer risk. (PubMed id 19237606)1, 4 Michiels S....Benhamou S. (Carcinogenesis 2009)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 6118 HGNC: 10290 AceView: RPA2 Ensembl:ENSG00000117748 euGenes: HUgn6118
    ECgene: RPA2 Kegg: 6118 H-InvDB: RPA2

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for RPA2 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for RPA2 Genetics and Cytogenetics in Oncology and Haematology
    NIEHS-SNPshttp://egp.gs.washington.edu/data/rpa2/

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for RPA2 gene:
    Search GeneIP for patents involving RPA2

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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