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Aliases for RPA2 Gene

Aliases for RPA2 Gene

  • Replication Protein A2 2 3 5
  • Replication Protein A 34 KDa Subunit 3 4
  • Replication Factor A Protein 2 3 4
  • Replication Protein A2, 32kDa 2 3
  • RF-A Protein 2 3 4
  • RP-A P32 3 4
  • RP-A P34 3 4
  • REPA2 3 4
  • RPA32 3 4
  • Replication Protein A2 (32kD) 2
  • RPA34 4

External Ids for RPA2 Gene

Previous GeneCards Identifiers for RPA2 Gene

  • GC01P027225
  • GC01M027451
  • GC01M027822
  • GC01M028090
  • GC01M028218
  • GC01M026473

Summaries for RPA2 Gene

GeneCards Summary for RPA2 Gene

RPA2 (Replication Protein A2) is a Protein Coding gene. Diseases associated with RPA2 include xeroderma pigmentosum, group a and ataxia-telangiectasia. Among its related pathways are Gene Expression and Cellular response to heat stress. GO annotations related to this gene include nucleic acid binding and ubiquitin protein ligase binding. An important paralog of this gene is RPA4.

UniProtKB/Swiss-Prot for RPA2 Gene

  • As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.

Gene Wiki entry for RPA2 Gene

No data available for Entrez Gene Summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for RPA2 Gene

Genomics for RPA2 Gene

Regulatory Elements for RPA2 Gene

Promoters for RPA2 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around RPA2 on UCSC Golden Path with GeneCards custom track

Genomic Location for RPA2 Gene

Chromosome:
1
Start:
27,891,524 bp from pter
End:
27,914,797 bp from pter
Size:
23,274 bases
Orientation:
Minus strand

Genomic View for RPA2 Gene

Genes around RPA2 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
RPA2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for RPA2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RPA2 Gene

Proteins for RPA2 Gene

  • Protein details for RPA2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P15927-RFA2_HUMAN
    Recommended name:
    Replication protein A 32 kDa subunit
    Protein Accession:
    P15927
    Secondary Accessions:
    • Q52II0
    • Q5TEI9
    • Q5TEJ5

    Protein attributes for RPA2 Gene

    Size:
    270 amino acids
    Molecular mass:
    29247 Da
    Quaternary structure:
    • Component of the replication protein A complex (RPA/RP-A), a heterotrimeric complex composed of RPA1, RPA2 and RPA3 (PubMed:2406247, PubMed:19116208, PubMed:10449415). Interacts with PRPF19; the PRP19-CDC5L complex is recruited to the sites of DNA repair where it ubiquitinates the replication protein A complex (RPA) (PubMed:24332808). Interacts with SERTAD3 (PubMed:10982866). Interacts with TIPIN (PubMed:17141802, PubMed:17296725). Interacts with TIMELESS (PubMed:17141802). Interacts with PPP4R2; the interaction is direct, DNA damage-dependent and mediates the recruitment of the PP4 catalytic subunit PPP4C (PubMed:20154705). Interacts (hyperphosphorylated) with RAD51 (PubMed:20154705). Interacts with SMARCAL1; the interaction is direct and mediates the recruitment to the RPA complex of SMARCAL1 (PubMed:19793861, PubMed:19793862, PubMed:19793863). Interacts with RAD52 and XPA; those interactions are direct and associate RAD52 and XPA to the RPA complex (PubMed:7700386, PubMed:8702565, PubMed:17765923, PubMed:11081631). Interacts with FBXO18 (PubMed:23319600).

    Three dimensional structures from OCA and Proteopedia for RPA2 Gene

    Alternative splice isoforms for RPA2 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for RPA2 Gene

Proteomics data for RPA2 Gene at MOPED

Post-translational modifications for RPA2 Gene

  • Differentially phosphorylated throughout the cell cycle, becoming phosphorylated at the G1-S transition and dephosphorylated in late mitosis. Mainly phosphorylated at Ser-23 and Ser-29, by cyclin A-CDK2 and cyclin B-CDK1, respectively during DNA replication and mitosis. Dephosphorylation may require the serine/threonine-protein phosphatase 4. Phosphorylation at Ser-23 and Ser-29 is a prerequisite for further phosphorylation. Becomes hyperphosphorylated on additional residues including Ser-4, Ser-8, Thr-21 and Ser-33 in response to DNA damage. Hyperphosphorylation is mediated by ATM, ATR and PRKDC. Primarily recruited to DNA repair nuclear foci as a hypophosphorylated form it undergoes subsequent hyperphosphorylation, catalyzed by ATR. Hyperphosphorylation is required for RAD51 recruitment to chromatin and efficient DNA repair. Phosphorylation at Thr-21 depends upon RFWD3 presence.
  • DNA damage-induced Lys-63-linked polyubiquitination by PRPF19 mediates ATRIP recruitment to the RPA complex at sites of DNA damage and activation of ATR.
  • Ubiquitination at Lys 127
  • Modification sites at PhosphoSitePlus

Other Protein References for RPA2 Gene

No data available for DME Specific Peptides for RPA2 Gene

Domains & Families for RPA2 Gene

Protein Domains for RPA2 Gene

Suggested Antigen Peptide Sequences for RPA2 Gene

Graphical View of Domain Structure for InterPro Entry

P15927

UniProtKB/Swiss-Prot:

RFA2_HUMAN :
  • Contains 1 OB DNA-binding domain.
  • Belongs to the replication factor A protein 2 family.
Domain:
  • Contains 1 OB DNA-binding domain.
Family:
  • Belongs to the replication factor A protein 2 family.
genes like me logo Genes that share domains with RPA2: view

No data available for Gene Families for RPA2 Gene

Function for RPA2 Gene

Molecular function for RPA2 Gene

GENATLAS Biochemistry:
replication protein A,subunit 2,component of a trimeric single strand binding protein,involved in replication,homologous recombination and nucleotide excision repair,colocolizing with RAD51 on synapsed axes in meiosis and facilitating RAD51 function,also colocalizing with BLM in meiotic prophase of prophase nuclei of spermatocytes
UniProtKB/Swiss-Prot Function:
As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.
UniProtKB/Swiss-Prot Induction:
Translationally up-regulated in response to DNA damage (at protein level).

Gene Ontology (GO) - Molecular Function for RPA2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003697 single-stranded DNA binding ISS --
GO:0031625 ubiquitin protein ligase binding IPI 21504906
genes like me logo Genes that share ontologies with RPA2: view
genes like me logo Genes that share phenotypes with RPA2: view

Animal Model Products

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for RPA2 Gene

Localization for RPA2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for RPA2 Gene

Nucleus. Nucleus, PML body. Note=Redistributes to discrete nuclear foci upon DNA damage in an ATR-dependent manner. {ECO:0000269 PubMed:12814551}.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for RPA2 Gene COMPARTMENTS Subcellular localization image for RPA2 gene
Compartment Confidence
nucleus 5
cytosol 2

Gene Ontology (GO) - Cellular Components for RPA2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000784 nuclear chromosome, telomeric region IDA 19135898
GO:0016605 colocalizes_with PML body IDA 17959650
genes like me logo Genes that share ontologies with RPA2: view

Pathways & Interactions for RPA2 Gene

SuperPathways for RPA2 Gene

Superpath Contained pathways
1 Telomere C-strand (Lagging Strand) Synthesis
2 Translesion synthesis by Y family DNA polymerases bypasses lesions on DNA template
3 DNA Double-Strand Break Repair
4 Transcription-Coupled Nucleotide Excision Repair (TC-NER)
5 CDK-mediated phosphorylation and removal of Cdc6
.78
genes like me logo Genes that share pathways with RPA2: view

SIGNOR curated interactions for RPA2 Gene

Activates:
Is activated by:
Other effect:

Gene Ontology (GO) - Biological Process for RPA2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000082 G1/S transition of mitotic cell cycle TAS --
GO:0000278 mitotic cell cycle TAS --
GO:0000724 double-strand break repair via homologous recombination IMP,TAS 17765923
GO:0006281 DNA repair TAS --
GO:0006283 transcription-coupled nucleotide-excision repair TAS --
genes like me logo Genes that share ontologies with RPA2: view

Drugs & Compounds for RPA2 Gene

(11) Drugs for RPA2 Gene - From: Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials

(6) Additional Compounds for RPA2 Gene - From: Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
genes like me logo Genes that share compounds with RPA2: view

Transcripts for RPA2 Gene

Unigene Clusters for RPA2 Gene

Replication protein A2, 32kDa:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for RPA2 Gene

ExUns: 1a · 1b · 1c ^ 2a · 2b ^ 3 ^ 4 ^ 5 ^ 6 ^ 7a · 7b ^ 8 ^ 9
SP1: - -
SP2:
SP3: -

Relevant External Links for RPA2 Gene

GeneLoc Exon Structure for
RPA2
ECgene alternative splicing isoforms for
RPA2

Expression for RPA2 Gene

mRNA expression in normal human tissues for RPA2 Gene

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for RPA2 Gene



SOURCE GeneReport for Unigene cluster for RPA2 Gene Hs.79411

genes like me logo Genes that share expression patterns with RPA2: view

Protein tissue co-expression partners for RPA2 Gene

- Elite partner

Primer Products

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues , Protein differential expression in normal tissues and mRNA Expression by UniProt/SwissProt for RPA2 Gene

Orthologs for RPA2 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for RPA2 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia RPA2 36
  • 75 (a)
OneToOne
RPA2 35
  • 98.27 (n)
  • 97.77 (a)
cow
(Bos Taurus)
Mammalia RPA2 36
  • 93 (a)
OneToOne
RPA2 35
  • 90.99 (n)
  • 93.33 (a)
dog
(Canis familiaris)
Mammalia RPA2 35
  • 90.86 (n)
  • 91.48 (a)
RPA2 36
  • 84 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Rpa2 16
Rpa2 36
  • 88 (a)
OneToOne
Rpa2 35
  • 85.56 (n)
  • 88.52 (a)
oppossum
(Monodelphis domestica)
Mammalia -- 36
  • 83 (a)
ManyToMany
-- 36
  • 83 (a)
ManyToMany
platypus
(Ornithorhynchus anatinus)
Mammalia -- 36
  • 67 (a)
OneToMany
rat
(Rattus norvegicus)
Mammalia Rpa2 35
  • 87.16 (n)
  • 88.89 (a)
chicken
(Gallus gallus)
Aves RPA2 35
  • 67.6 (n)
  • 71.49 (a)
RPA2 36
  • 70 (a)
OneToMany
lizard
(Anolis carolinensis)
Reptilia -- 36
  • 63 (a)
OneToMany
African clawed frog
(Xenopus laevis)
Amphibia Xl.5629 35
tropical clawed frog
(Silurana tropicalis)
Amphibia rpa2 35
  • 64.32 (n)
  • 65.56 (a)
Str.6420 35
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.8565 35
zebrafish
(Danio rerio)
Actinopterygii rpa2 36
  • 57 (a)
OneToMany
rpa2 35
  • 61.26 (n)
  • 56.98 (a)
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP008332 35
  • 41.36 (n)
  • 29.46 (a)
fruit fly
(Drosophila melanogaster)
Insecta RPA2 35
  • 43.93 (n)
  • 33.05 (a)
RPA2 36
  • 28 (a)
OneToMany
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_ADL176W 35
  • 42.82 (n)
  • 28.88 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes RFA2 35
  • 42.25 (n)
  • 32.89 (a)
RFA2 36
  • 25 (a)
OneToMany
RFA2 38
K. lactis yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0E07371g 35
  • 44.33 (n)
  • 29.26 (a)
thale cress
(Arabidopsis thaliana)
eudicotyledons RPA2 35
  • 45.47 (n)
  • 35.09 (a)
rice
(Oryza sativa)
Liliopsida Os02g0829100 35
  • 43.85 (n)
  • 32.37 (a)
bread mold
(Neurospora crassa)
Ascomycetes NCU07717 35
  • 46.14 (n)
  • 38.02 (a)
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes ssb2 35
  • 47.51 (n)
  • 37.28 (a)
sea squirt
(Ciona savignyi)
Ascidiacea CSA.6662 36
  • 32 (a)
OneToMany
Species with no ortholog for RPA2:
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for RPA2 Gene

ENSEMBL:
Gene Tree for RPA2 (if available)
TreeFam:
Gene Tree for RPA2 (if available)

Paralogs for RPA2 Gene

Paralogs for RPA2 Gene

(1) SIMAP similar genes for RPA2 Gene using alignment to 3 proteins:

Pseudogenes.org Pseudogenes for RPA2 Gene

genes like me logo Genes that share paralogs with RPA2: view

Variants for RPA2 Gene

Sequence variations from dbSNP and Humsavar for RPA2 Gene

SNP ID Clin Chr 01 pos Sequence Context AA Info Type
rs1000431 -- 27,898,603(+) tggag(C/T)ggcat intron-variant
rs1000432 -- 27,898,608(+) cggca(C/T)gatct intron-variant
rs1884469 -- 27,912,923(-) TCTGC(C/T)GTTGC intron-variant
rs2064708 -- 27,904,813(-) tgagc(C/T)gggtg intron-variant
rs2064709 -- 27,904,649(-) aaaaT(A/G)CGGTT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for RPA2 Gene

Variant ID Type Subtype PubMed ID
nsv509069 CNV Insertion 20534489
nsv871591 CNV Gain 21882294
nsv870553 CNV Gain 21882294
nsv871458 CNV Gain 21882294

Variation tolerance for RPA2 Gene

Residual Variation Intolerance Score: 42.1% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.72; 46.57% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for RPA2 Gene

HapMap Linkage Disequilibrium report
RPA2
Human Gene Mutation Database (HGMD)
RPA2

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for RPA2 Gene

Disorders for RPA2 Gene

MalaCards: The human disease database

(2) MalaCards diseases for RPA2 Gene - From: DISEASES, Novoseek, and GeneCards

Disorder Aliases PubMed IDs
xeroderma pigmentosum, group a
  • xeroderma pigmentosum, type 1
ataxia-telangiectasia
  • ataxia telangiectasia
- elite association - COSMIC cancer census association via MalaCards
Search RPA2 in MalaCards View complete list of genes associated with diseases

Relevant External Links for RPA2

Genetic Association Database (GAD)
RPA2
Human Genome Epidemiology (HuGE) Navigator
RPA2
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
RPA2
genes like me logo Genes that share disorders with RPA2: view

No data available for UniProtKB/Swiss-Prot and Genatlas for RPA2 Gene

Publications for RPA2 Gene

  1. A PP4 phosphatase complex dephosphorylates RPA2 to facilitate DNA repair via homologous recombination. (PMID: 20154705) Lee D.H. … Chowdhury D. (Nat. Struct. Mol. Biol. 2010) 3 4 23 67
  2. The role of RPA2 phosphorylation in homologous recombination in response to replication arrest. (PMID: 20130019) Shi W. … Zhang J. (Carcinogenesis 2010) 3 23
  3. Physical interaction between replication protein A (RPA) and MRN: involvement of RPA2 phosphorylation and the N-terminus of RPA1. (PMID: 19586055) Oakley G.G. … Patrick S.M. (Biochemistry 2009) 3 23
  4. Ionizing radiation-dependent and independent phosphorylation of the 32-kDa subunit of replication protein A during mitosis. (PMID: 19671522) Stephan H. … Nasheuer H.P. (Nucleic Acids Res. 2009) 3 23
  5. Protein phosphatase 2A-dependent dephosphorylation of replication protein A is required for the repair of DNA breaks induced by replication stress. (PMID: 19704001) Feng J. … Wang X.F. (Mol. Cell. Biol. 2009) 3 23

Products for RPA2 Gene

Sources for RPA2 Gene

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