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Aliases for RPA2 Gene

Aliases for RPA2 Gene

  • Replication Protein A2, 32kDa 2 3
  • Replication Protein A 34 KDa Subunit 3 4
  • Replication Factor A Protein 2 3 4
  • RF-A Protein 2 3 4
  • RP-A P32 3 4
  • RP-A P34 3 4
  • REPA2 3 4
  • RPA32 3 4
  • Replication Protein A 32 KDa Subunit 3
  • Replication Protein A2 (32kD) 2
  • RPA34 4

External Ids for RPA2 Gene

Summaries for RPA2 Gene

GeneCards Summary for RPA2 Gene

RPA2 (Replication Protein A2, 32kDa) is a Protein Coding gene. Diseases associated with RPA2 include xeroderma pigmentosum, group a. Among its related pathways are Cell Cycle, Mitotic and Cell Cycle, Mitotic. GO annotations related to this gene include protein N-terminus binding and protein phosphatase binding. An important paralog of this gene is RPA4.

UniProtKB/Swiss-Prot for RPA2 Gene

  • As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.

Gene Wiki entry for RPA2 Gene

No data available for Entrez Gene Summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for RPA2 Gene

Genomics for RPA2 Gene

Genomic Location for RPA2 Gene

Start:
27,891,524 bp from pter
End:
27,914,797 bp from pter
Size:
23,274 bases
Orientation:
Minus strand

Genomic View for RPA2 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for RPA2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RPA2 Gene

Regulatory Elements for RPA2 Gene

Proteins for RPA2 Gene

  • Protein details for RPA2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P15927-RFA2_HUMAN
    Recommended name:
    Replication protein A 32 kDa subunit
    Protein Accession:
    P15927
    Secondary Accessions:
    • Q52II0
    • Q5TEI9
    • Q5TEJ5

    Protein attributes for RPA2 Gene

    Size:
    270 amino acids
    Molecular mass:
    29247 Da
    Quaternary structure:
    • Component of the replication protein A complex (RPA/RP-A), a heterotrimeric complex composed of RPA1, RPA2 and RPA3. Interacts with PRPF19; the PRP19-CDC5L complex is recruited to the sites of DNA repair where it ubiquitinates the replication protein A complex (RPA). Interacts with SERTAD3. Interacts with TIPIN. Interacts with TIMELESS. Interacts with PPP4R2; the interaction is direct, DNA damage-dependent and mediates the recruitment of the PP4 catalytic subunit PPP4C. Interacts (hyperphosphorylated) with RAD51. Interacts with SMARCAL1; the interaction is direct and mediates the recruitment to the RPA complex of SMARCAL1. Interacts with RAD52 and XPA; those interactions are direct and associate RAD52 and XPA to the RPA complex.

    Three dimensional structures from OCA and Proteopedia for RPA2 Gene

    Alternative splice isoforms for RPA2 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for RPA2 Gene

Proteomics data for RPA2 Gene at MOPED

Post-translational modifications for RPA2 Gene

  • Differentially phosphorylated throughout the cell cycle, becoming phosphorylated at the G1-S transition and dephosphorylated in late mitosis. Mainly phosphorylated at Ser-23 and Ser-29, by cyclin A-CDK2 and cyclin B-CDK1, respectively during DNA replication and mitosis. Dephosphorylation may require the serine/threonine-protein phosphatase 4. Phosphorylation at Ser-23 and Ser-29 is a prerequisite for further phosphorylation. Becomes hyperphosphorylated on additional residues including Ser-4, Ser-8, Thr-21 and Ser-33 in response to DNA damage. Hyperphosphorylation is mediated by ATM, ATR and PRKDC. Primarily recruited to DNA repair nuclear foci as a hypophosphorylated form it undergoes subsequent hyperphosphorylation, catalyzed by ATR. Hyperphosphorylation is required for RAD51 recruitment to chromatin and efficient DNA repair. Phosphorylation at Thr-21 depends upon RFWD3 presence.
  • DNA damage-induced Lys-63-linked polyubiquitination by PRPF19 mediates ATRIP recruitment to the RPA complex at sites of DNA damage and activation of ATR.
  • Modification sites at PhosphoSitePlus
  • Ubiquitination at Lys127

Other Protein References for RPA2 Gene

No data available for DME Specific Peptides for RPA2 Gene

Domains for RPA2 Gene

Protein Domains for RPA2 Gene

UniProtKB/Swiss-Prot:

RFA2_HUMAN
Domain:
  • Contains 1 OB DNA-binding domain.:
    • P15927
Family:
  • Belongs to the replication factor A protein 2 family.:
    • P15927
genes like me logo Genes that share domains with RPA2: view

No data available for Gene Families for RPA2 Gene

Function for RPA2 Gene

Molecular function for RPA2 Gene

GENATLAS Biochemistry: replication protein A,subunit 2,component of a trimeric single strand binding protein,involved in replication,homologous recombination and nucleotide excision repair,colocolizing with RAD51 on synapsed axes in meiosis and facilitating RAD51 function,also colocalizing with BLM in meiotic prophase of prophase nuclei of spermatocytes
UniProtKB/Swiss-Prot Function: As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.
UniProtKB/Swiss-Prot Induction: Translationally up-regulated in response to DNA damage (at protein level).

Gene Ontology (GO) - Molecular Function for RPA2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003676 nucleic acid binding --
GO:0003677 DNA binding --
GO:0003684 damaged DNA binding IDA 7700386
GO:0003697 single-stranded DNA binding ISS --
GO:0005515 protein binding IPI 10449415
genes like me logo Genes that share ontologies with RPA2: view
genes like me logo Genes that share phenotypes with RPA2: view

No data available for Enzyme Numbers (IUBMB) , Animal Models , Transcription Factor Targeting and HOMER Transcription for RPA2 Gene

Localization for RPA2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for RPA2 Gene

Nucleus. Nucleus, PML body. Note=Redistributes to discrete nuclear foci upon DNA damage in an ATR-dependent manner.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for RPA2 Gene COMPARTMENTS Subcellular localization image for RPA2 gene
Compartment Confidence
nucleus 5
cytosol 2

Gene Ontology (GO) - Cellular Components for RPA2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000781 colocalizes_with chromosome, telomeric region IDA 17959650
GO:0000785 chromatin IDA 10336450
GO:0005634 nucleus IDA 10982866
GO:0005654 nucleoplasm TAS --
GO:0005662 DNA replication factor A complex IPI 16135809
genes like me logo Genes that share ontologies with RPA2: view

Pathways for RPA2 Gene

SuperPathways for RPA2 Gene

Superpath Contained pathways
1 Telomere C-strand (Lagging Strand) Synthesis
2 Homologous Recombination Repair
3 CDK-mediated phosphorylation and removal of Cdc6
.78
4 Global Genomic NER (GG-NER)
5 DNA Repair
genes like me logo Genes that share pathways with RPA2: view

Pathways by source for RPA2 Gene

Gene Ontology (GO) - Biological Process for RPA2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000082 G1/S transition of mitotic cell cycle TAS --
GO:0000278 mitotic cell cycle TAS --
GO:0000718 nucleotide-excision repair, DNA damage removal TAS --
GO:0000722 telomere maintenance via recombination TAS --
GO:0000723 telomere maintenance TAS --
genes like me logo Genes that share ontologies with RPA2: view

Compounds for RPA2 Gene

(17) Novoseek inferred chemical compound relationships for RPA2 Gene

Compound -log(P) Hits PubMed IDs
rpa 2 98.3 118
adozelesin 73.6 6
camptothecin 72.1 18
aphidicolin 69.7 7
7-hydroxystaurosporine 65.2 1
genes like me logo Genes that share compounds with RPA2: view

Transcripts for RPA2 Gene

Unigene Clusters for RPA2 Gene

Replication protein A2, 32kDa:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for RPA2 Gene

ExUns: 1a · 1b · 1c ^ 2a · 2b ^ 3 ^ 4 ^ 5 ^ 6 ^ 7a · 7b ^ 8 ^ 9
SP1: - -
SP2:
SP3: -

Relevant External Links for RPA2 Gene

GeneLoc Exon Structure for
RPA2
ECgene alternative splicing isoforms for
RPA2

Expression for RPA2 Gene

mRNA expression in normal human tissues for RPA2 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for RPA2 Gene

SOURCE GeneReport for Unigene cluster for RPA2 Gene Hs.79411

genes like me logo Genes that share expressions with RPA2: view

Orthologs for RPA2 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for RPA2 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia RPA2 36
  • 98.27 (n)
  • 97.77 (a)
RPA2 37
  • 75 (a)
OneToOne
cow
(Bos Taurus)
Mammalia RPA2 36
  • 90.99 (n)
  • 93.33 (a)
RPA2 37
  • 93 (a)
OneToOne
dog
(Canis familiaris)
Mammalia RPA2 36
  • 90.86 (n)
  • 91.48 (a)
RPA2 37
  • 84 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Rpa2 36
  • 85.56 (n)
  • 88.52 (a)
Rpa2 16
Rpa2 37
  • 88 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia -- 37
  • 83 (a)
ManyToMany
-- 37
  • 83 (a)
ManyToMany
platypus
(Ornithorhynchus anatinus)
Mammalia -- 37
  • 67 (a)
OneToMany
rat
(Rattus norvegicus)
Mammalia Rpa2 36
  • 87.16 (n)
  • 88.89 (a)
chicken
(Gallus gallus)
Aves RPA2 36
  • 67.6 (n)
  • 71.49 (a)
RPA2 37
  • 70 (a)
OneToMany
lizard
(Anolis carolinensis)
Reptilia -- 37
  • 63 (a)
OneToMany
African clawed frog
(Xenopus laevis)
Amphibia Xl.5629 36
tropical clawed frog
(Silurana tropicalis)
Amphibia rpa2 36
  • 64.32 (n)
  • 65.56 (a)
Str.6420 36
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.8565 36
zebrafish
(Danio rerio)
Actinopterygii rpa2 36
  • 61.26 (n)
  • 56.98 (a)
rpa2 37
  • 57 (a)
OneToMany
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP008332 36
  • 41.36 (n)
  • 29.46 (a)
fruit fly
(Drosophila melanogaster)
Insecta RPA2 36
  • 43.93 (n)
  • 33.05 (a)
RPA2 37
  • 28 (a)
OneToMany
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_ADL176W 36
  • 42.82 (n)
  • 28.88 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes RFA2 36
  • 42.25 (n)
  • 32.89 (a)
RFA2 37
  • 25 (a)
OneToMany
RFA2 39
K. lactis yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0E07371g 36
  • 44.33 (n)
  • 29.26 (a)
thale cress
(Arabidopsis thaliana)
eudicotyledons RPA2 36
  • 45.47 (n)
  • 35.09 (a)
rice
(Oryza sativa)
Liliopsida Os02g0829100 36
  • 43.85 (n)
  • 32.37 (a)
bread mold
(Neurospora crassa)
Ascomycetes NCU07717 36
  • 46.14 (n)
  • 38.02 (a)
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes ssb2 36
  • 47.51 (n)
  • 37.28 (a)
sea squirt
(Ciona savignyi)
Ascidiacea CSA.6662 37
  • 32 (a)
OneToMany
Species with no ortholog for RPA2:
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for RPA2 Gene

ENSEMBL:
Gene Tree for RPA2 (if available)
TreeFam:
Gene Tree for RPA2 (if available)

Paralogs for RPA2 Gene

Paralogs for RPA2 Gene

Selected SIMAP similar genes for RPA2 Gene using alignment to 3 proteins:

Pseudogenes.org Pseudogenes for RPA2 Gene

genes like me logo Genes that share paralogs with RPA2: view

Variants for RPA2 Gene

Sequence variations from dbSNP and Humsavar for RPA2 Gene

SNP ID Clin Chr 01 pos Sequence Context AA Info Type MAF
rs7356 -- 27,891,589(-) ACTGC(A/G)TTTTG utr-variant-3-prime
rs1000431 -- 27,898,603(+) tggag(C/T)ggcat intron-variant
rs1000432 -- 27,898,608(+) cggca(C/T)gatct intron-variant
rs1884469 -- 27,912,923(-) TCTGC(C/T)GTTGC intron-variant
rs2064708 -- 27,904,813(-) tgagc(C/T)gggtg intron-variant

Structural Variations from Database of Genomic Variants (DGV) for RPA2 Gene

Variant ID Type Subtype PubMed ID
nsv509069 CNV Insertion 20534489
nsv871591 CNV Gain 21882294
nsv870553 CNV Gain 21882294
nsv871458 CNV Gain 21882294

Relevant External Links for RPA2 Gene

HapMap Linkage Disequilibrium report
RPA2
Human Gene Mutation Database (HGMD)
RPA2

Disorders for RPA2 Gene

(1) University of Copenhagen DISEASES for RPA2 Gene

(9) Novoseek inferred disease relationships for RPA2 Gene

Disease -log(P) Hits PubMed IDs
ataxia telangiectasia 77.2 6
xeroderma pigmentosum 42.2 1
promyelocytic leukemia 29.6 1
colon cancer 0 3
breast cancer 0 1

Relevant External Links for RPA2

Genetic Association Database (GAD)
RPA2
Human Genome Epidemiology (HuGE) Navigator
RPA2
genes like me logo Genes that share disorders with RPA2: view

No data available for UniProtKB/Swiss-Prot for RPA2 Gene

Publications for RPA2 Gene

  1. cdc2 family kinases phosphorylate a human cell DNA replication factor, RPA, and activate DNA replication. (PMID: 1318195) Dutta A. … Stillman B. (EMBO J. 1992) 3 4 23
  2. Sites of UV-induced phosphorylation of the p34 subunit of replication protein A from HeLa cells. (PMID: 9295339) Zernik-Kobak M. … Dixon K. (J. Biol. Chem. 1997) 3 4 23
  3. RBT1, a novel transcriptional co-activator, binds the second subunit of replication protein A. (PMID: 10982866) Cho J.M. … Alaoui-Jamali M.A. (Nucleic Acids Res. 2000) 3 4 23
  4. ATR kinase activity regulates the intranuclear translocation of ATR and RPA following ionizing radiation. (PMID: 12814551) Barr S.M. … Cimprich K.A. (Curr. Biol. 2003) 3 4 23
  5. Structure of the RPA trimerization core and its role in the multistep DNA-binding mechanism of RPA. (PMID: 11927569) Bochkareva E. … Bochkarev A. (EMBO J. 2002) 3 4 23

Products for RPA2 Gene

  • Addgene plasmids for RPA2

Sources for RPA2 Gene

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