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RPA1 Gene

protein-coding   GIFtS: 70
GCID: GC17P001680

Replication Protein A1, 70kDa

(Previous name: replication protein A1 (70kD))
  See RPA1-related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Replication Protein A1, 70kDa1 2     Replication Protein A1 (70kD)1
Replication Factor A Protein 12 3     HSSB2
Single-Stranded DNA-Binding Protein2 3     MST0752
REPA12 3     MSTP0752
RPA702 3     RF-A2
RF-A Protein 12 3     RP-A2
RP-A P702 3     Replication Protein A 70 KDa DNA-Binding Subunit2

External Ids:    HGNC: 102891   Entrez Gene: 61172   Ensembl: ENSG000001323837   OMIM: 1798355   UniProtKB: P276943   

Export aliases for RPA1 gene to outside databases

Previous GC identifers: GC17P002039 GC17P001684 GC17P001939


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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GeneCards Summary for RPA1 Gene:
RPA1 (replication protein A1, 70kDa) is a protein-coding gene. Diseases associated with RPA1 include werner syndrome, and xeroderma pigmentosum. GO annotations related to this gene include chromatin binding and single-stranded DNA binding.

UniProtKB/Swiss-Prot: RFA1_HUMAN, P27694
Function: Plays an essential role in several cellular processes in DNA metabolism including replication,
recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus
prevents complementary DNA from reannealing
Function: Functions as component of the alternative replication protein A complex (aRPA). aRPA binds
single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and
cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha
but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual
incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange

Gene Wiki entry for RPA1 (Replication protein A1) Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000017.10  NC_018928.2  NT_010718.17  
Regulatory elements:
   Regulatory transcription factor binding sites in the RPA1 gene promoter:
         MAZR   CBF-C   CREB   CBF-A   CBF-B   deltaCREB   CP1A   CP1C   NF-Y   CBF(2)   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidRPA1 promoter sequence
   Search Chromatin IP Primers for RPA1

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat RPA1


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 17p13.3   Ensembl cytogenetic band:  17p13.3   HGNC cytogenetic band: 17p13.3

RPA1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
RPA1 gene location

GeneLoc information about chromosome 17         GeneLoc Exon Structure

GeneLoc location for GC17P001680:  view genomic region     (about GC identifiers)

Start:
1,732,996 bp from pter      End:
1,803,376 bp from pter
Size:
70,381 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: RFA1_HUMAN, P27694 (See protein sequence)
Recommended Name: Replication protein A 70 kDa DNA-binding subunit  
Size: 616 amino acids; 68138 Da
Subunit: Heterotrimer composed of RPA1, RPA2 and RPA3 (canonical replication protein A complex). Component of the
alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. The DNA-binding activity may
reside exclusively on the RPA1 subunit. Interacts with RIPK1 and XPA. Interacts with RPA4. Interacts with the
polymerase alpha subunit POLA1/p180; this interaction stabilizes the replicative complex and reduces the
misincorporation rate of DNA polymerase alpha by acting as a fidelity clamp. Interacts with RAD51 and SENP6 to
regulate DNA repair. Interacts with HELB; this interaction promotes HELB recruitment to chromatin following DNA
damage. Interacts with PRIMPOL
Sequence caution: Sequence=BAD92969.1; Type=Erroneous initiation; Note=Translation N-terminally shortened;
Selected PDB 3D structures from and Proteopedia for RPA1 (see all 18):
1EWI (3D)        1FGU (3D)        1JMC (3D)        1L1O (3D)        2B29 (3D)        2B3G (3D)    
Secondary accessions: A8K0Y9 Q59ES9

Explore the universe of human proteins at neXtProt for RPA1: NX_P27694

Explore proteomics data for RPA1 at MOPED

Post-translational modifications: 

  • Sumoylated on lysine residues Lys-449 and Lys-577, with Lys-449 being the major site. Sumoylation promotes
    recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombination. Desumoylated
    by SENP61
  • Ubiquitination2 at Lys22, Lys88, Lys157, Lys163, Lys167, Lys183, Lys196, Lys244, Lys367, Lys410,
                                 Lys431, Lys443, Lys489, Lys502, Lys588, Lys595
  • Modification sites at PhosphoSitePlus

  • See RPA1 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_002936.1  
    ENSEMBL proteins: 
     ENSP00000461733   ENSP00000459788   ENSP00000254719   ENSP00000461466  
    Reactome Protein details: P27694

    RPA1 Human Recombinant Protein Products:

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    Novus Biologicals RPA1 Protein
    Novus Biologicals RPA1 Lysates
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for RPA1

     
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    RPA1 Antibody Products:

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    RPA1 Assay Products:

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    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for RPA1
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    Search eBioscience for ELISAs for RPA1 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    5 InterPro protein domains:
     IPR012340 NA-bd_OB-fold
     IPR004365 NA-bd_OB_tRNA
     IPR013955 Rep_factor-A_C
     IPR004591 Rep_factor_Rpa1
     IPR007199 Rep_factor-A_N

    Graphical View of Domain Structure for InterPro Entry P27694

    ProtoNet protein and cluster: P27694

    3 Blocks protein domains:
    IPB004365 OB-fold nucleic acid binding domain
    IPB007199 Replication factor-A protein 1
    IPB013955 Replication factor-A


    UniProtKB/Swiss-Prot: RFA1_HUMAN, P27694
    Similarity: Belongs to the replication factor A protein 1 family
    Similarity: Contains 1 OB DNA-binding domain


    Find genes that share domains with RPA1           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: RFA1_HUMAN, P27694
    Function: Plays an essential role in several cellular processes in DNA metabolism including replication,
    recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus
    prevents complementary DNA from reannealing
    Function: Functions as component of the alternative replication protein A complex (aRPA). aRPA binds
    single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and
    cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha
    but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual
    incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange

         Genatlas biochemistry entry for RPA1:
    replication protein A,subunit 1,component 70kDa of a trimeric single strand binding protein,involved in
    replication,homologous recombination and nucleotide excision repair,colocalizing with RAD51 on synapsed axes in
    meiosis and facilitating RAD51 function,also colocalizing with BLM in meiotic prophase of prophase nuclei of
    spermatocytes

         Gene Ontology (GO): Selected molecular function terms (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003676nucleic acid binding ----
    GO:0003677DNA binding ----
    GO:0003682chromatin binding IEA--
    GO:0003697single-stranded DNA binding IEA--
    GO:0005515protein binding IPI10373438
         
    Find genes that share ontologies with RPA1           About GenesLikeMe


    Phenotypes:
         8 GenomeRNAi human phenotypes for RPA1:
     Bright nuclei  Decreased viability with cispl  Increased S DNA content, incre  Increased Salmonella enterica  
     Increased cell number in S and  Increased gamma-H2AX phosphory  Increased number of cells in m  S arrest 

         5 MGI mutant phenotypes (inferred from 2 alleles(MGI details for Rpa1):
     cellular  hematopoietic system  immune system  mortality/aging  tumorigenesis 

    Find genes that share phenotypes with RPA1           About GenesLikeMe

    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for RPA1
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for RPA1

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for RPA1
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for RPA1

    miRNA
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    miRTarBase miRNAs that target RPA1:
    hsa-mir-615-3p (MIRT039750), hsa-let-7e-5p (MIRT051422), hsa-mir-1301-3p (MIRT036052), hsa-mir-339-5p (MIRT042750), hsa-mir-31-5p (MIRT049867)

    Block miRNA regulation of human, mouse, rat RPA1 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate RPA1 (see all 15):
    hsa-miR-345 hsa-miR-4264 hsa-miR-361-5p hsa-miR-3616-5p hsa-miR-622 hsa-miR-3647-5p hsa-miR-3074-3p hsa-miR-151-3p
    SwitchGear 3'UTR luciferase reporter plasmidRPA1 3' UTR sequence
    Inhib. RNA
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    Predesigned siRNA for gene silencing in human, mouse, rat RPA1

    Gene Editing
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    Clone
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    OriGene clones in human, mouse for RPA1 (see all 8)
    OriGene ORF clones in mouse, rat for RPA1
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    GenScript: all cDNA clones in your preferred vector: RPA1 (NM_002945)
    Sino Biological Human cDNA Clone for RPA1
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for RPA1
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RPA1

    Cell Line
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    Flow Cytometry
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    eBioscience FlowRNA Probe Sets ( VA1-12221) for RPA1 


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    RFA1_HUMAN, P27694: Nucleus
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus5
    cytoskeleton4
    cytosol3
    mitochondrion1

    Gene Ontology (GO): Selected cellular component terms (see all 11):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000793condensed chromosome ----
    GO:0000794condensed nuclear chromosome ----
    GO:0000800lateral element IEA--
    GO:0001673male germ cell nucleus IEA--
    GO:0005634nucleus IDA--

    Find genes that share ontologies with RPA1           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for RPA1 About   (see all 16)  
    See pathways by source

    SuperPathSelected contained pathways About (see all per SuperPath)
    1Telomere C-strand (Lagging Strand) Synthesis
    Gap-filling DNA repair synthesis and ligation in TC-NER0.94
    Mismatch repair0.00
    Repair synthesis of patch ~27-30 bases long by DNA polymerase0.94
    Lagging Strand Synthesis0.00
    Telomere C-strand (Lagging Strand) Synthesis0.79
    Removal of the Flap Intermediate0.00
    Extension of Telomeres0.79
    Processive synthesis on the C-strand of the telomere0.00
    2Activation of the pre-replicative complex
    Activation of the pre-replicative complex0.66
    Cell cycle Start of DNA replication in early S phase0.53
    DNA Replication0.66
    3Global Genomic NER (GG-NER)
    Global Genomic NER (GG-NER)0.69
    Dual incision reaction in GG-NER0.62
    Nucleotide excision repair0.69
    Formation of incision complex in GG-NER0.62
    4E2F mediated regulation of DNA replication
    G1/S Transition0.81
    G1 to S cell cycle control0.35
    Mitotic G1-G1/S phases0.81
    5DNA strand elongation
    DNA strand elongation0.63
    DNA replication0.63


    Find genes that share SuperPaths with RPA1           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    2 Cell Signaling Technology (CST) Pathways for RPA1
        Cell Cycle / Checkpoint Control
    DNA Damage

    1 GeneGo (Thomson Reuters) Pathway for RPA1
        Cell cycle Start of DNA replication in early S phase

    5 BioSystems Pathways for RPA1
        G1 to S cell cycle control
    Mismatch repair
    Homologous recombination
    DNA Replication
    RB in Cancer


    Selected Reactome Pathways for RPA1 (see all 14)
        Regulation of HSF1-mediated heat shock response
    Repair synthesis for gap-filling by DNA polymerase in TC-NER
    Activation of ATR in response to replication stress
    Removal of the Flap Intermediate
    Processing of DNA double-strand break ends


    5 Kegg Pathways  (Kegg details for RPA1):
        DNA replication
    Nucleotide excision repair
    Mismatch repair
    Homologous recombination
    Fanconi anemia pathway

        Pathway & Disease-focused RT2 Profiler PCR Arrays including RPA1: 
              DNA Damage Signaling Pathway in human mouse rat
              DNA Repair in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for RPA1

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for RPA1 (P276941, 2, 3 ENSP000002547194) via UniProtKB, MINT, STRING, and/or I2D (see all 412)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    HIST1H4AP628053, ENSP000003529804I2D: score=3 STRING: ENSP00000352980
    HIST1H4CP628053, ENSP000003670344I2D: score=3 STRING: ENSP00000367034
    HIST1H4DP628053, ENSP000003432824I2D: score=3 STRING: ENSP00000343282
    HIST1H4EP628053, ENSP000003536244I2D: score=3 STRING: ENSP00000353624
    HIST1H4HP628053, ENSP000002893524I2D: score=3 STRING: ENSP00000289352
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 23):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000082G1/S transition of mitotic cell cycle TAS--
    GO:0000278mitotic cell cycle TAS--
    GO:0000718nucleotide-excision repair, DNA damage removal TAS--
    GO:0000722telomere maintenance via recombination TAS--
    GO:0000723telomere maintenance TAS--

    Find genes that share ontologies with RPA1           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for RPA1 (RFA1)

    Selected Novoseek inferred chemical compound relationships for RPA1 gene (see all 26)    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    rpa 2 89.1 28 17361204 (6), 19586055 (4), 15856006 (2), 8020972 (2) (see all 10)
    dbds 66 6 15047856 (2), 14596605 (1), 10473346 (1), 17583916 (1)
    cpds 54.8 1 11705987 (1)
    hydroxyurea 42.8 10 18003706 (3), 15180989 (2), 15735006 (1), 15653682 (1)
    methylmethanesulfonate 39.1 3 12697761 (1), 9539419 (1)
    oligonucleotide 35.1 9 15102447 (1), 16234232 (1), 9461638 (1), 16481746 (1) (see all 6)
    camptothecin 34.3 10 10064605 (3), 15653682 (2), 15856006 (1), 15929725 (1)
    phosphatidylinositol-3,4,5-trisphosphate 30.9 2 9052858 (1), 9427642 (1)
    danazol 30.3 3 7849064 (2), 9600915 (1)
    bpde 30.1 1 15362978 (1)



    Find genes that share compounds with RPA1           About GenesLikeMe



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for RPA1 gene: 
    NM_002945.3  

    Unigene Cluster for RPA1:

    Replication protein A1, 70kDa
    Hs.461925  [show with all ESTs]
    Unigene Representative Sequence: NM_002945
    7 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000571058 ENST00000570451 ENST00000254719(uc002fto.2) ENST00000571725
    ENST00000573924 ENST00000574049 ENST00000573994
    miRNA
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    hsa-miR-345 hsa-miR-4264 hsa-miR-361-5p hsa-miR-3616-5p hsa-miR-622 hsa-miR-3647-5p hsa-miR-3074-3p hsa-miR-151-3p
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    GenScript: all cDNA clones in your preferred vector: RPA1 (NM_002945)
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    Additional mRNA sequence: 

    AB209732.1 AF167994.1 AK289704.1 BC018126.1 M63488.1 

    16 DOTS entries:

    DT.453758  DT.100820639  DT.436781  DT.120966270  DT.120966324  DT.407661  DT.100820638  DT.100820642 
    DT.95220953  DT.120966329  DT.95146636  DT.100647566  DT.91834116  DT.92446560  DT.95220906  DT.95238864 

    Selected AceView cDNA sequences (see all 387):

    BM541370 BM456944 BM456314 BQ023839 BE292972 AI458104 BM847443 BM848213 
    BG036436 BM845370 BE773977 BQ004759 BM542894 CB121937 BM848384 Z40763 
    BG826869 BE898956 BF740840 BQ218906 AA991598 CR615851 BM825910 CR592316 

    GeneLoc Exon Structure

    3 Alternative Splicing Database (ASD) splice patterns (SP) for RPA1    About this scheme

    ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15a · 15b ^ 16 ^ 17a · 17b
    SP1:                                                                                      -                           
    SP2:                                                                                -     -                           
    SP3:                                                                                                                  


    ECgene alternative splicing isoforms for RPA1

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

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    RPA1 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: GAAAGGATTT
    RPA1 Expression
    About this image


    RPA1 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 7) fully expand
     
     Brain (Nervous System)    fully expand to see all 6 entries
             Cerebral Cortex
     
     Neural Tube (Nervous System)    fully expand to see all 4 entries
             Metencephalon
     
     Ovary (Reproductive System)    fully expand to see all 2 entries
             Ovarian Mesenchymal Stroma Cells Ovary Interstitium
             Oviduct
     
     Trophoblast (Extraembryonic Tissues)
             Trophoblast Cells Trophoblast
     
     Pancreas (Endocrine System)
             Islets of Langerhans
    RPA1 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    RPA1 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.461925
        Pathway & Disease-focused RT2 Profiler PCR Arrays including RPA1: 
              DNA Damage Signaling Pathway in human mouse rat
              DNA Repair in human mouse rat

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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of eukaryotes.

    Orthologs for RPA1 gene from Selected species (see all 23)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Rpa11 , 5 replication protein A11, 5 84.39(n)1
    85.32(a)1
      11 (45.79 cM)5
    682751  NM_001164223.11  NP_001157695.11 
     752981665 
    chicken
    (Gallus gallus)
    Aves RPA11 replication protein A1, 70kDa 74.24(n)
    74.84(a)
      417563  NM_001006221.1  NP_001006221.1 
    lizard
    (Anolis carolinensis)
    Reptilia RPA16
    replication protein A1, 70kDa
    65(a)
    1 ↔ 1
    GL343393.1(14226-47260)
    African clawed frog
    (Xenopus laevis)
    Amphibia LOC3979372 replication protein A (RPA) 76.4(n)    X67240.1 
    zebrafish
    (Danio rerio)
    Actinopterygii wufi14b082 wufi14b08 73.36(n)   327491  BC044372.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta RpA-701 , 3 DNA dependent DNA replication
    single-stranded more3
    Replication Protein A 701
    41(a)3
    51.2(n)1
    45.36(a)1
      3 84F63
    409721  NM_079550.31  NP_524274.11 
    worm
    (Caenorhabditis elegans)
    Secernentea F18A1.53 replication factor A 31(a)   II(7664513-7666854)   --
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes RFA1(YAR007C)4
    RFA11
    Subunit of heterotrimeric Replication Protein A (RPA), more4
    RFA11
    43.97(n)1
    32.56(a)1
      1(158619-156754)4
    8512661, 4  NP_009404.11, 4 
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons AT4G191301 AT4G19130 49.22(n)
    40.85(a)
      827651  NM_118032.2  NP_567576.2 
    rice
    (Oryza sativa)
    Liliopsida Os05g01110001 Os05g0111000 50.15(n)
    41.19(a)
      4337594  NM_001060980.1  NP_001054445.1 


    ENSEMBL Gene Tree for RPA1 (if available)
    TreeFam Gene Tree for RPA1 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section

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      --

    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for RPA1 (see all 1732)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 17 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs681493361,2
    C--1661911(+) CCGTA-/TTTTTTTT
    /TTTTTTTTTT
    TTTTT
    1 -- int11NA 2
    rs1928067831,2
    --1735189(+) TGCCCG/TGCCTT 1 -- int10--------
    rs2009580401,2
    --1735248(+) CCATA-/CCCCCC 1 -- int10--------
    rs350639561,2
    C--1735444(+) GGTCTC/TGAACT 1 -- int10--------
    rs561578021,2
    C--1735469(+) ATCCAA/CCCTCC 1 -- int13Minor allele frequency- C:0.00NA CSA 5
    rs1837427611,2
    --1735577(+) TTCCCC/GACACT 1 -- int10--------
    rs1500110311,2
    --1735598(+) ATCTTC/TGTATC 1 -- int10--------
    rs116554541,2
    C,A,H--1735640(+) AAATCC/TCTTTG 1 -- int14Minor allele frequency- T:0.16NA WA EA 360
    rs1439992471,2
    --1735758(+) TCACTA/GCAACC 1 -- int10--------
    rs116518241,2
    C,F,H--1735853(+) tttttG/Atattt 1 -- int12Minor allele frequency- A:0.50NA 4

    HapMap Linkage Disequilibrium report for RPA1 (1732996 - 1803376 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 1 variation for RPA1:    About this table    
    Variant IDTypeSubtypePubMed ID
    esv33335CNV Loss17666407

    Human Gene Mutation Database (HGMD): RPA1
    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing RPA1
    DNA2.0 Custom Variant and Variant Library Synthesis for RPA1

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 179835    OMIM disorders: --

    2 diseases for RPA1:    
    About MalaCards
    werner syndrome    xeroderma pigmentosum

    1 disease from the University of Copenhagen DISEASES database for RPA1:
    Xeroderma pigmentosum

    Find genes that share disorders with RPA1           About GenesLikeMe

    Selected Novoseek inferred disease relationships for RPA1 gene (see all 19)    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    xeroderma pigmentosum 80.3 19 8972858 (1), 10626358 (1), 10340474 (1), 10828957 (1) (see all 15)
    werner syndrome 71.2 1 15965237 (1)
    nijmegen breakage syndrome 61.5 1 18003706 (1)
    ataxia telangiectasia 49.5 2 15929725 (1)
    genetic disorder 6.08 3 8293499 (1)
    metastasis 1.89 1 17361204 (1)
    lupus erythematosus systemic 0 1 12472178 (1)
    leukemia 0 1 9279361 (1)
    necrosis 0 5 16700705 (1), 19786940 (1), 16113842 (1), 1601521 (1)
    breast cancer 0 1 15102447 (1)

    Genetic Association Database (GAD): RPA1
    Human Genome Epidemiology (HuGE) Navigator: RPA1 (10 documents)

    Export disorders for RPA1 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for RPA1 gene, integrated from 10 sources (see all 409):
    (articles sorted by number of sources associating them with RPA1)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Replication protein A1 reduces transcription of the endothelial nitric oxide synthase gene containing a -786T--&gt;C mutation associated with coronary spastic angina. (PubMed id 11063722)1, 4, 9 Miyamoto Y....Nakao K. (Hum. Mol. Genet. 2000)
    2. Cloning, overexpression, and genomic mapping of the 14-kDa subunit of human replication protein A. (PubMed id 8454588)1, 3, 9 Umbricht C.B. and Kelly T.J. (J. Biol. Chem. 1993)
    3. Polymorphism discovery in 62 DNA repair genes and haplotype associations with risks for lung and head and neck cancers. (PubMed id 17494052)1, 4, 9 Michiels S....Benhamou S. (Carcinogenesis 2007)
    4. Human DNA helicase B (HDHB) binds to replication protein A and facilitates cellular recovery from replication stress. (PubMed id 22194613)1, 2 Guler G.D.... Fanning E. (J. Biol. Chem. 2012)
    5. Comprehensive screen of genetic variation in DNA repair pathway genes and postmenopausal breast cancer risk. (PubMed id 20496165)1, 4 Monsees G.M....Han J. (Breast Cancer Res. Treat. 2011)
    6. Identification of neuroglycan C and interacting partners as potential susceptibility genes for schizophrenia in a Southern Chinese population. (PubMed id 19367581)1, 4 So H.C....Sham P.C. (Am. J. Med. Genet. B Neuropsychiatr. Genet. 2010)
    7. MicroRNA-related genetic variations as predictors for risk of second primary tumor and/or recurrence in patients with early-stage head and neck cancer. (PubMed id 20819778)1, 4 Zhang X....Wu X. (Carcinogenesis 2010)
    8. XPC genetic polymorphisms correlate with the response to imatinib treatment in patients with chronic phase chronic myeloid leukemia. (PubMed id 20575039)1, 4 Guillem V.M....HernA!ndez-Boluda J.C. (Am. J. Hematol. 2010)
    9. Variation within DNA repair pathway genes and risk of multiple sclerosis. (PubMed id 20522537)1, 4 Briggs F.B....Barcellos L.F. (Am. J. Epidemiol. 2010)
    10. Genetic polymorphisms in 85 DNA repair genes and bladder cancer risk. (PubMed id 19237606)1, 4 Michiels S....Benhamou S. (Carcinogenesis 2009)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 6117 HGNC: 10289 AceView: RPA1 Ensembl:ENSG00000132383 euGenes: HUgn6117
    ECgene: RPA1 Kegg: 6117 H-InvDB: RPA1

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

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    NameDescription
    PharmGKB entry for RPA1 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for RPA1 Genetics and Cytogenetics in Oncology and Haematology
    NIEHS-SNPshttp://egp.gs.washington.edu/data/rpa1/

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

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    Patent Information for RPA1 gene:
    Search GeneIP for patents involving RPA1

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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