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Aliases for RPA1 Gene

Aliases for RPA1 Gene

  • Replication Protein A1 2 3 5
  • Single-Stranded DNA-Binding Protein 3 4
  • Replication Factor A Protein 1 3 4
  • Replication Protein A1, 70kDa 2 3
  • RF-A Protein 1 3 4
  • RP-A P70 3 4
  • REPA1 3 4
  • RPA70 3 4
  • Replication Protein A1 (70kD) 2
  • MSTP075 3
  • MST075 3
  • HSSB 3
  • RF-A 3
  • RP-A 3

External Ids for RPA1 Gene

Previous GeneCards Identifiers for RPA1 Gene

  • GC17P002039
  • GC17P001684
  • GC17P001939
  • GC17P001680

Summaries for RPA1 Gene

GeneCards Summary for RPA1 Gene

RPA1 (Replication Protein A1) is a Protein Coding gene. Diseases associated with RPA1 include werner syndrome and xeroderma pigmentosum, group a. Among its related pathways are Gene Expression and Transport to the Golgi and subsequent modification. GO annotations related to this gene include nucleic acid binding and single-stranded DNA binding.

UniProtKB/Swiss-Prot for RPA1 Gene

  • As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage (PubMed:9430682). In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response (PubMed:24332808). It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage (PubMed:17765923). Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair (PubMed:7697716). Plays also a role in base excision repair (BER) probably through interaction with UNG (PubMed:9765279). Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance (PubMed:17959650). As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange (PubMed:19996105).

Gene Wiki entry for RPA1 Gene

No data available for Entrez Gene Summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for RPA1 Gene

Genomics for RPA1 Gene

Regulatory Elements for RPA1 Gene

Promoters for RPA1 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around RPA1 on UCSC Golden Path with GeneCards custom track

Genomic Location for RPA1 Gene

Chromosome:
17
Start:
1,829,702 bp from pter
End:
1,900,082 bp from pter
Size:
70,381 bases
Orientation:
Plus strand

Genomic View for RPA1 Gene

Genes around RPA1 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
RPA1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for RPA1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RPA1 Gene

Proteins for RPA1 Gene

  • Protein details for RPA1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P27694-RFA1_HUMAN
    Recommended name:
    Replication protein A 70 kDa DNA-binding subunit
    Protein Accession:
    P27694
    Secondary Accessions:
    • A8K0Y9
    • Q59ES9

    Protein attributes for RPA1 Gene

    Size:
    616 amino acids
    Molecular mass:
    68138 Da
    Quaternary structure:
    • Component of the canonical replication protein A complex (RPA), an heterotrimer composed of RPA1, RPA2 and RPA3. Also component of the aRPA, the alternative replication protein A complex, a trimeric complex similar to the replication protein A complex/RPA but where RPA1 and RPA3 are associated with RPA4 instead of RPA2. The DNA-binding activity may reside exclusively on the RPA1 subunit. Interacts with PRPF19; the PRP19-CDC5L complex is recruited to the sites of DNA repair where it ubiquitinates the replication protein A complex (RPA). Interacts with RIPK1. Interacts with the polymerase alpha subunit POLA1/p180; this interaction stabilizes the replicative complex and reduces the misincorporation rate of DNA polymerase alpha by acting as a fidelity clamp. Interacts with RAD51 and SENP6 to regulate DNA repair. Interacts with HELB; this interaction promotes HELB recruitment to chromatin following DNA damage. Interacts with PRIMPOL. Interacts with XPA; the interaction is direct and associates XPA with the RPA complex.
    SequenceCaution:
    • Sequence=BAD92969.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for RPA1 Gene

neXtProt entry for RPA1 Gene

Proteomics data for RPA1 Gene at MOPED

Post-translational modifications for RPA1 Gene

  • DNA damage-induced Lys-63-linked polyubiquitination by PRPF19 mediates ATRIP recruitment to the RPA complex at sites of DNA damage and activation of ATR.
  • Sumoylated on lysine residues Lys-449 and Lys-577, with Lys-449 being the major site. Sumoylation promotes recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombination. Desumoylated by SENP6.
  • Ubiquitination at Lys 22, Lys 88, Lys 157, Lys 163, Lys 167, Lys 183, Lys 196, Lys 244, Lys 367, Lys 410, Lys 431, Lys 443, Lys 489, Lys 502, Lys 588, and Lys 595
  • Modification sites at PhosphoSitePlus

Other Protein References for RPA1 Gene

No data available for DME Specific Peptides for RPA1 Gene

Domains & Families for RPA1 Gene

Suggested Antigen Peptide Sequences for RPA1 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

P27694

UniProtKB/Swiss-Prot:

RFA1_HUMAN :
  • Contains 1 OB DNA-binding domain.
  • Belongs to the replication factor A protein 1 family.
Domain:
  • Contains 1 OB DNA-binding domain.
Family:
  • Belongs to the replication factor A protein 1 family.
genes like me logo Genes that share domains with RPA1: view

No data available for Gene Families for RPA1 Gene

Function for RPA1 Gene

Molecular function for RPA1 Gene

GENATLAS Biochemistry:
replication protein A,subunit 1,component 70kDa of a trimeric single strand binding protein,involved in replication,homologous recombination and nucleotide excision repair,colocalizing with RAD51 on synapsed axes in meiosis and facilitating RAD51 function,also colocalizing with BLM in meiotic prophase of prophase nuclei of spermatocytes
UniProtKB/Swiss-Prot Function:
As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage (PubMed:9430682). In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response (PubMed:24332808). It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage (PubMed:17765923). Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair (PubMed:7697716). Plays also a role in base excision repair (BER) probably through interaction with UNG (PubMed:9765279). Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance (PubMed:17959650). As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange (PubMed:19996105).

Gene Ontology (GO) - Molecular Function for RPA1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003697 single-stranded DNA binding IEA,IDA 8990123
genes like me logo Genes that share ontologies with RPA1: view
genes like me logo Genes that share phenotypes with RPA1: view

Animal Models for RPA1 Gene

MGI Knock Outs for RPA1:
  • Rpa1 tm1b(KOMP)Wtsi

Animal Model Products

Inhibitory RNA Products

In Situ Assay Products

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for RPA1 Gene

Localization for RPA1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for RPA1 Gene

Nucleus. Nucleus, PML body. Note=Enriched in PML bodies in cells displaying alternative lengthening of their telomeres. {ECO:0000269 PubMed:17959650}.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for RPA1 Gene COMPARTMENTS Subcellular localization image for RPA1 gene
Compartment Confidence
nucleus 5
cytosol 2
mitochondrion 1

Gene Ontology (GO) - Cellular Components for RPA1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0016605 colocalizes_with PML body IDA 17959650
genes like me logo Genes that share ontologies with RPA1: view

Pathways & Interactions for RPA1 Gene

SuperPathways for RPA1 Gene

Superpath Contained pathways
1 Telomere C-strand (Lagging Strand) Synthesis
2 Translesion synthesis by Y family DNA polymerases bypasses lesions on DNA template
3 DNA Double-Strand Break Repair
4 Transcription-Coupled Nucleotide Excision Repair (TC-NER)
5 CDK-mediated phosphorylation and removal of Cdc6
.78
genes like me logo Genes that share pathways with RPA1: view

Gene Ontology (GO) - Biological Process for RPA1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000082 G1/S transition of mitotic cell cycle TAS --
GO:0000278 mitotic cell cycle TAS --
GO:0000724 double-strand break repair via homologous recombination IMP,TAS 17765923
GO:0006260 DNA replication IEA,TAS --
GO:0006261 DNA-dependent DNA replication TAS 8756712
genes like me logo Genes that share ontologies with RPA1: view

No data available for SIGNOR curated interactions for RPA1 Gene

Drugs & Compounds for RPA1 Gene

(22) Drugs for RPA1 Gene - From: Novoseek and ApexBio

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Capecitabine Approved, Investigational Pharma RNA processing inhibitor and thymidylate synthase inhibitor, F-pyrimidines 1220
Nelarabine Approved, Investigational Pharma Prodrug of ara-G for T-LBL/T-ALL 0
Oxaliplatin Approved, Investigational Pharma Antitumor agent, Platinum 1322
Uridine Experimental Pharma 0
Carmofur Withdrawn Pharma Cytostatic derivative of fluorouracilm,antineoplatic agent 0

(14) Additional Compounds for RPA1 Gene - From: Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs

(10) ApexBio Compounds for RPA1 Gene

Compound Action Cas Number
Adenine sulfate Sulfate salt form of adenine 321-30-2
Blasticidin S HCl 3513-03-9
Capecitabine 154361-50-9
Carmofur Cytostatic derivative of fluorouracilm,antineoplatic agent 61422-45-5
Gemcitabine HCl Inhibits DNA synthesis,deoxycytidine analog 122111-03-9
Mercaptopurine (6-MP) Purine synthesis inhibitor 50-44-2
Nelarabine Prodrug of ara-G for T-LBL/T-ALL 121032-29-9
Oxaliplatin Antitumor agent 61825-94-3
Rifaximin (Xifaxan) RNA synthesis inhibitor, PXR activator 80621-81-4
Uridine 58-96-8
genes like me logo Genes that share compounds with RPA1: view

Transcripts for RPA1 Gene

Unigene Clusters for RPA1 Gene

Replication protein A1, 70kDa:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for RPA1 Gene

ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15a · 15b ^ 16 ^ 17a · 17b
SP1: -
SP2: - -
SP3:

Relevant External Links for RPA1 Gene

GeneLoc Exon Structure for
RPA1
ECgene alternative splicing isoforms for
RPA1

Expression for RPA1 Gene

mRNA expression in normal human tissues for RPA1 Gene

Protein differential expression in normal tissues from HIPED for RPA1 Gene

This gene is overexpressed in Peripheral blood mononuclear cells (14.6).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for RPA1 Gene



SOURCE GeneReport for Unigene cluster for RPA1 Gene Hs.461925

genes like me logo Genes that share expression patterns with RPA1: view

Protein tissue co-expression partners for RPA1 Gene

- Elite partner

Primer Products

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues and mRNA Expression by UniProt/SwissProt for RPA1 Gene

Orthologs for RPA1 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for RPA1 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia RPA1 35
  • 87.45 (n)
  • 90.1 (a)
RPA1 36
  • 90 (a)
OneToOne
dog
(Canis familiaris)
Mammalia RPA1 35
  • 88.62 (n)
  • 89.27 (a)
RPA1 36
  • 89 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Rpa1 35
  • 84.39 (n)
  • 85.32 (a)
Rpa1 16
Rpa1 36
  • 82 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia RPA1 35
  • 99.24 (n)
  • 99.51 (a)
RPA1 36
  • 100 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Rpa1 35
  • 84.89 (n)
  • 87.5 (a)
oppossum
(Monodelphis domestica)
Mammalia RPA1 36
  • 83 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia RPA1 36
  • 82 (a)
OneToOne
chicken
(Gallus gallus)
Aves RPA1 35
  • 74.24 (n)
  • 74.84 (a)
RPA1 36
  • 74 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia RPA1 36
  • 65 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia rpa1 35
  • 71 (n)
  • 75.67 (a)
Str.10339 35
African clawed frog
(Xenopus laevis)
Amphibia LOC397937 35
zebrafish
(Danio rerio)
Actinopterygii rpa1 35
  • 64.55 (n)
  • 65.22 (a)
wufi14b08 35
rpa1 36
  • 65 (a)
OneToOne
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.4321 35
fruit fly
(Drosophila melanogaster)
Insecta RpA-70 37
  • 41 (a)
RpA-70 35
  • 51.2 (n)
  • 45.36 (a)
RpA-70 36
  • 44 (a)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP001421 35
  • 51.58 (n)
  • 45.61 (a)
worm
(Caenorhabditis elegans)
Secernentea F18A1.5 37
  • 31 (a)
rpa-1 36
  • 26 (a)
OneToOne
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_AFL008W 35
  • 48.93 (n)
  • 39.86 (a)
K. lactis yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0A08844g 35
  • 48.2 (n)
  • 38.5 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes RFA1 35
  • 43.97 (n)
  • 32.56 (a)
RFA1 36
  • 31 (a)
OneToOne
RFA1 38
thale cress
(Arabidopsis thaliana)
eudicotyledons AT4G19130 35
  • 49.22 (n)
  • 40.85 (a)
rice
(Oryza sativa)
Liliopsida Os05g0111000 35
  • 50.15 (n)
  • 41.19 (a)
bread mold
(Neurospora crassa)
Ascomycetes NCU03606 35
  • 49.26 (n)
  • 39.29 (a)
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes ssb1 35
  • 48.89 (n)
  • 39.69 (a)
Species with no ortholog for RPA1:
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for RPA1 Gene

ENSEMBL:
Gene Tree for RPA1 (if available)
TreeFam:
Gene Tree for RPA1 (if available)

Paralogs for RPA1 Gene

No data available for Paralogs for RPA1 Gene

Variants for RPA1 Gene

Sequence variations from dbSNP and Humsavar for RPA1 Gene

SNP ID Clin Chr 17 pos Sequence Context AA Info Type
rs5030755 - 1,879,658(+) TGGAC(A/G)CATCC reference, missense
rs9082 -- 1,897,969(+) TCATG(C/T)ATTTT utr-variant-3-prime
rs10100 -- 1,897,942(-) AGGTA(A/G)AAAAA utr-variant-3-prime
rs12727 -- 1,897,771(+) TCAGG(C/G)TTTAA utr-variant-3-prime
rs17734 -- 1,897,850(-) GGGAC(A/G)GCAAG utr-variant-3-prime

Structural Variations from Database of Genomic Variants (DGV) for RPA1 Gene

Variant ID Type Subtype PubMed ID
esv33335 CNV Loss 17666407

Variation tolerance for RPA1 Gene

Residual Variation Intolerance Score: 17.7% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 3.16; 51.64% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for RPA1 Gene

HapMap Linkage Disequilibrium report
RPA1
Human Gene Mutation Database (HGMD)
RPA1

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for RPA1 Gene

Disorders for RPA1 Gene

MalaCards: The human disease database

(5) MalaCards diseases for RPA1 Gene - From: DISEASES, Novoseek, and GeneCards

Disorder Aliases PubMed IDs
werner syndrome
  • adult premature ageing syndrome
xeroderma pigmentosum, group a
  • xeroderma pigmentosum, type 1
xeroderma pigmentosum, variant type
  • de sanctis-cacchione syndrome
atrophic rhinitis
  • dry rhinitis
middle cerebral artery infarction
  • infarction middle cerebral artery
- elite association - COSMIC cancer census association via MalaCards
Search RPA1 in MalaCards View complete list of genes associated with diseases

Relevant External Links for RPA1

Genetic Association Database (GAD)
RPA1
Human Genome Epidemiology (HuGE) Navigator
RPA1
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
RPA1
genes like me logo Genes that share disorders with RPA1: view

No data available for UniProtKB/Swiss-Prot and Genatlas for RPA1 Gene

Publications for RPA1 Gene

  1. RPA mediates recombination repair during replication stress and is displaced from DNA by checkpoint signalling in human cells. (PMID: 17765923) Sleeth K.M. … Helleday T. (J. Mol. Biol. 2007) 3 4 23 67
  2. Cloning, overexpression, and genomic mapping of the 14-kDa subunit of human replication protein A. (PMID: 8454588) Umbricht C.B. … Kelly T.J. (J. Biol. Chem. 1993) 2 3 23
  3. Expression of mutant RPA in human cancer cells causes telomere shortening. (PMID: 20139621) Kobayashi Y. … Ueno M. (Biosci. Biotechnol. Biochem. 2010) 3 23
  4. Replication protein A stimulates the Werner syndrome protein branch migration activity. (PMID: 19812417) Sowd G. … Opresko P.L. (J. Biol. Chem. 2009) 3 23
  5. Human RPA phosphorylation by ATR stimulates DNA synthesis and prevents ssDNA accumulation during DNA-replication stress. (PMID: 19843584) Vassin V.M. … Borowiec J.A. (J. Cell. Sci. 2009) 3 23

Products for RPA1 Gene

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