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Aliases for ROCK2 Gene

Aliases for ROCK2 Gene

  • Rho-Associated, Coiled-Coil Containing Protein Kinase 2 2 3
  • Rho-Associated, Coiled-Coil-Containing Protein Kinase II 3 4
  • P164 ROCK-2 3 4
  • EC 2.7.11.1 4 64
  • ROCK-II 3 4
  • Rho-Associated, Coiled-Coil-Containing Protein Kinase 2 4
  • Rho-Associated Protein Kinase 2 3
  • Rho Kinase 2 4
  • EC 2.7.11 64
  • KIAA0619 4

External Ids for ROCK2 Gene

Summaries for ROCK2 Gene

Entrez Gene Summary for ROCK2 Gene

  • The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008]

GeneCards Summary for ROCK2 Gene

ROCK2 (Rho-Associated, Coiled-Coil Containing Protein Kinase 2) is a Protein Coding gene. Among its related pathways are Signaling by GPCR and Proteoglycans in cancer. GO annotations related to this gene include protein serine/threonine kinase activity and structural molecule activity. An important paralog of this gene is STK38L.

UniProtKB/Swiss-Prot for ROCK2 Gene

  • Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.

Tocris Summary for ROCK2 Gene

  • Rho-kinases (aka Rho-associated coiled-coil kinases or ROCKs) are serine/threonine kinases that are activated by RhoA GTPases, and are key modulators of processes involving cytoskeletal rearrangement such focal adhesion formation, cell motility and tumor cell invasion. There are two isoforms of ROCK, ROCKI (aka ROKbeta or p160ROCK) and ROCKII (aka ROKalpha), which share a high degree of structural identity in their kinase domains (92%). The two Rho-kinase isoforms have different patterns of tissue expression; ROCKII expression is highest in the brain and in muscle whereas ROCKI has a ubiquitous tissue distribution. Subcellularly, ROCKII is found in the cytosol and localizes to the cleavage furrow during cytokinesis. ROCKI is thought to colocalize with the centrosomes. ROCKs phosphorylate a number of proteins involved in actin filament assembly and contraction (e.g. LIM Kinases, MLCK, ERM proteins). Other downstream targets of the Rho kinases include signal transduction molecules such as IRS-1 and PI 3-K/AKT.

No data available for Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for ROCK2 Gene

Genomics for ROCK2 Gene

Genomic Location for ROCK2 Gene

Start:
11,179,761 bp from pter
End:
11,348,330 bp from pter
Size:
168,570 bases
Orientation:
Minus strand

Genomic View for ROCK2 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for ROCK2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for ROCK2 Gene

Regulatory Elements for ROCK2 Gene

Proteins for ROCK2 Gene

  • Protein details for ROCK2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    O75116-ROCK2_HUMAN
    Recommended name:
    Rho-associated protein kinase 2
    Protein Accession:
    O75116
    Secondary Accessions:
    • Q53QZ0
    • Q53SJ7
    • Q9UQN5

    Protein attributes for ROCK2 Gene

    Size:
    1388 amino acids
    Molecular mass:
    160900 Da
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    • Homodimer. Interacts with IRS1, RHOB and RHOC (By similarity). Interacts with RHOA (activated by GTP), PPP1R12A, CHORDC1, SORL1, EP300 and BRCA2. Interacts with NPM1 and this interaction enhances its activity. Interacts with RAF1 (By similarity).
    SequenceCaution:
    • Sequence=AAX93049.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; Sequence=BAA31594.2; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for ROCK2 Gene

neXtProt entry for ROCK2 Gene

Proteomics data for ROCK2 Gene at MOPED

Post-translational modifications for ROCK2 Gene

  • Cleaved by granzyme B during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing
  • Phosphorylation at Tyr-722 reduces its binding to RHOA and is crucial for focal adhesion dynamics. Dephosphorylation by PTPN11 stimulates its RHOA binding activity.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for ROCK2 Gene

ENSEMBL proteins:
Reactome Protein details:
REFSEQ proteins:

Domains for ROCK2 Gene

Gene Families for ROCK2 Gene

UniProtKB/Swiss-Prot:

ROCK2_HUMAN
Domain:
  • An interaction between Thr-414 and Asp-48 is essential for kinase activity and dimerization.:
    • O75116
  • Contains 1 AGC-kinase C-terminal domain.:
    • O75116
  • Contains 1 PH domain.:
    • O75116
  • Contains 1 protein kinase domain.:
    • O75116
Family:
  • Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.:
    • O75116
Similarity:
  • Contains 1 phorbol-ester/DAG-type zinc finger.:
    • O75116
  • Contains 1 REM (Hr1) repeat.:
    • O75116
genes like me logo Genes that share domains with ROCK2: view

Function for ROCK2 Gene

Molecular function for ROCK2 Gene

GENATLAS Biochemistry: serine/threonine kinase ROCK2/rho kinase,isozyme of ROCK1,one the targets for the small GTPase Rho,putative mediator of the Rho-dependent signaling pathway
UniProtKB/Swiss-Prot CatalyticActivity: ATP + a protein = ADP + a phosphoprotein
UniProtKB/Swiss-Prot EnzymeRegulation: Activated by RHOA binding. Inhibited by Y-27632 (By similarity).
UniProtKB/Swiss-Prot Function: Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.

Enzyme Numbers (IUBMB) for ROCK2 Gene

Gene Ontology (GO) - Molecular Function for ROCK2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000166 nucleotide binding --
GO:0004672 protein kinase activity --
GO:0004674 protein serine/threonine kinase activity IDA 16574662
GO:0004713 protein tyrosine kinase activity --
GO:0005198 structural molecule activity NAS 9933571
genes like me logo Genes that share ontologies with ROCK2: view
genes like me logo Genes that share phenotypes with ROCK2: view

Animal Models for ROCK2 Gene

MGI Knock Outs for ROCK2:

miRNA for ROCK2 Gene

miRTarBase miRNAs that target ROCK2

No data available for Transcription Factor Targeting and HOMER Transcription for ROCK2 Gene

Localization for ROCK2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for ROCK2 Gene

Cytoplasm. Cell membrane; Peripheral membrane protein. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Cytoplasmic, and associated with actin microfilaments and the plasma membrane. {ECO:0000250}.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for ROCK2 Gene COMPARTMENTS Subcellular localization image for ROCK2 gene
Compartment Confidence
cytoskeleton 5
nucleus 5
plasma membrane 5
cytosol 4
extracellular 1
mitochondrion 1

Gene Ontology (GO) - Cellular Components for ROCK2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IEA --
GO:0005813 centrosome IDA 17015463
GO:0005829 cytosol TAS --
GO:0005886 plasma membrane IEA --
GO:0031616 spindle pole centrosome IEA --
genes like me logo Genes that share ontologies with ROCK2: view

Pathways for ROCK2 Gene

genes like me logo Genes that share pathways with ROCK2: view

Gene Ontology (GO) - Biological Process for ROCK2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000910 cytokinesis NAS 9933571
GO:0001843 neural tube closure IEA --
GO:0006468 protein phosphorylation IDA 18559669
GO:0006939 smooth muscle contraction TAS 12778124
GO:0007264 small GTPase mediated signal transduction TAS --
genes like me logo Genes that share ontologies with ROCK2: view

Compounds for ROCK2 Gene

(9) ApexBio Compounds for ROCK2 Gene

Compound Action Cas Number
chroman 1 highly potent and selective ROCK II inhibitor. [1273579-40-0]
Fasudil (HA-1077) HCl Cyclic nucleotide-dependent protein kinase inhibitor and Rho-associated kinase inhibitor [105628-07-7]
GSK429286A Selective Rho-kinase inhibitor [864082-47-3]
H-1152 more specific, stronger and membrane-permeable inhibitor of Rho-kinase [451462-58-1]
Hydroxyfasudil potent Rho-kinase inhibitor [105628-72-6]
Narciclasine modulates the Rho/ROCK/LIM kinase/cofilin pathway [29477-83-6]
RKI-1447 potent small molecule inhibitor of ROCK1 and ROCK2. [1342278-01-6]
SLx-2119 Selective ROCK2 inhibitor [911417-87-3]
Y-27632 dihydrochloride p160ROCK inhibitor [146986-50-7]

(5) Tocris Compounds for ROCK2 Gene

Compound Action Cas Number
Fasudil hydrochloride Inhibitor of cyclic nucleotide dependent- and Rho-kinases [103745-39-7]
GSK 269962 Potent and selective ROCK inhibitor [850664-21-0]
H 1152 dihydrochloride Selective Rho-kinase (ROCK) inhibitor [451462-58-1]
SB 772077B dihydrochloride Potent Rho-kinase inhibitor; vasodilator [607373-46-6]
Y-27632 dihydrochloride Selective p160ROCK inhibitor [146986-50-7]

(846) HMDB Compounds for ROCK2 Gene

Compound Synonyms Cas Number PubMed IDs
Adenosine triphosphate
  • 5'-(tetrahydrogen triphosphate) Adenosine
56-65-5
ADP
  • adenosindiphosphorsaeure
58-64-0
DG(14:0/14:0/0:0)
  • DAG(14:0/14:0)
Not Available
DG(14:0/14:1(9Z)/0:0)
  • DAG(14:0/14:1)
Not Available
DG(14:0/15:0/0:0)
  • Diacylglycerol(29:0)
Not Available

(2) Drugbank Compounds for ROCK2 Gene

genes like me logo Genes that share compounds with ROCK2: view

Transcripts for ROCK2 Gene

Unigene Clusters for ROCK2 Gene

Rho-associated, coiled-coil containing protein kinase 2:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for ROCK2 Gene

No ASD Table

Relevant External Links for ROCK2 Gene

GeneLoc Exon Structure for
ROCK2
ECgene alternative splicing isoforms for
ROCK2

Expression for ROCK2 Gene

mRNA expression in normal human tissues for ROCK2 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for ROCK2 Gene

SOURCE GeneReport for Unigene cluster for ROCK2 Gene Hs.681743

genes like me logo Genes that share expressions with ROCK2: view

Orthologs for ROCK2 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for ROCK2 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia ROCK2 36
  • 99.83 (n)
  • 99.86 (a)
ROCK2 37
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia ROCK2 36
  • 93.52 (n)
  • 97.77 (a)
ROCK2 37
  • 98 (a)
OneToOne
dog
(Canis familiaris)
Mammalia ROCK2 36
  • 92.17 (n)
  • 96.15 (a)
ROCK2 37
  • 97 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Rock2 36
  • 91.04 (n)
  • 96.69 (a)
Rock2 16
Rock2 37
  • 97 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia ROCK2 37
  • 90 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia ROCK2 37
  • 88 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Rock2 36
  • 90.73 (n)
  • 96.76 (a)
chicken
(Gallus gallus)
Aves ROCK2 36
  • 83.16 (n)
  • 91.54 (a)
ROCK2 37
  • 85 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia ROCK2 37
  • 84 (a)
OneToOne
African clawed frog
(Xenopus laevis)
Amphibia rocka-A-prov 36
tropical clawed frog
(Silurana tropicalis)
Amphibia rock2 36
  • 75.39 (n)
  • 83.32 (a)
Str.15799 36
zebrafish
(Danio rerio)
Actinopterygii rock2 36
ROCK2 (3 of 10) 37
  • 78 (a)
OneToMany
ROCK2 (4 of 10) 37
  • 79 (a)
OneToMany
ROCK2 (5 of 10) 37
  • 78 (a)
OneToMany
ROCK2 (6 of 10) 37
  • 81 (a)
OneToMany
ROCK2 (7 of 10) 37
  • 79 (a)
OneToMany
ROCK2 (8 of 10) 37
  • 73 (a)
OneToMany
ROCK2 (9 of 10) 37
  • 80 (a)
OneToMany
rock2a 36
  • 69.69 (n)
  • 76.79 (a)
rock2a 37
  • 76 (a)
OneToMany
rock2b 37
  • 65 (a)
OneToMany
rock2b 37
  • 64 (a)
OneToMany
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP000406 36
  • 48.97 (n)
  • 45.74 (a)
fruit fly
(Drosophila melanogaster)
Insecta rok 36
  • 49.79 (n)
  • 46.14 (a)
rok 37
  • 43 (a)
OneToOne
rok 38
  • 43 (a)
worm
(Caenorhabditis elegans)
Secernentea let-502 36
  • 52.17 (n)
  • 43.6 (a)
let-502 37
  • 36 (a)
OneToOne
let-502 38
  • 33 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes CBK1 37
  • 22 (a)
OneToMany
sea squirt
(Ciona savignyi)
Ascidiacea -- 37
  • 48 (a)
OneToOne
Species with no ortholog for ROCK2:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for ROCK2 Gene

ENSEMBL:
Gene Tree for ROCK2 (if available)
TreeFam:
Gene Tree for ROCK2 (if available)

Paralogs for ROCK2 Gene

genes like me logo Genes that share paralogs with ROCK2: view

Variants for ROCK2 Gene

Sequence variations from dbSNP and Humsavar for ROCK2 Gene

SNP ID Clin Chr 02 pos Sequence Context AA Info Type MAF
rs8996 -- 11,180,751(-) TTCTC(A/G)TTTTT utr-variant-3-prime
rs9349 -- 11,180,614(-) CTTAT(A/T)GGATT utr-variant-3-prime
rs372345 -- 11,199,916(+) CTACT(A/C/G/T)ACTGT intron-variant
rs372350 -- 11,199,921(+) TACTG(A/C/G/T)ACTAT intron-variant
rs373008 -- 11,200,053(+) AAAGT(C/T)GTTTT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for ROCK2 Gene

Variant ID Type Subtype PubMed ID
esv2592438 CNV Insertion 19546169
esv267549 CNV Insertion 20981092
esv272377 CNV Insertion 20981092
nsv526038 CNV Loss 19592680
nsv2607 CNV Loss 18451855
esv2658593 CNV Deletion 23128226
nsv436304 CNV Deletion 17901297
esv25473 CNV Loss 19812545
nsv498912 CNV Loss 21111241
nsv514052 CNV Loss 21397061
esv2543558 CNV Insertion 19546169
esv271091 CNV Insertion 20981092
esv272435 CNV Insertion 20981092
esv1339412 CNV Insertion 17803354
esv2453181 CNV Insertion 19546169
esv2662875 CNV Deletion 23128226

Relevant External Links for ROCK2 Gene

HapMap Linkage Disequilibrium report
ROCK2
Human Gene Mutation Database (HGMD)
ROCK2

Disorders for ROCK2 Gene

Relevant External Links for ROCK2

Genetic Association Database (GAD)
ROCK2
Human Genome Epidemiology (HuGE) Navigator
ROCK2
genes like me logo Genes that share disorders with ROCK2: view

No data available for UniProtKB/Swiss-Prot for ROCK2 Gene

Publications for ROCK2 Gene

  1. Nuclear Rho kinase, ROCK2, targets p300 acetyltransferase. (PMID: 16574662) Tanaka T. … Hamamori Y. (J. Biol. Chem. 2006) 3 4 23
  2. A major haplotype block at the rho-associated kinase 2 locus is associated with a lower risk of hypertension in a recessive manner: the HYPGENE study. (PMID: 18971541) Rankinen T. … Bouchard C. (Hypertens. Res. 2008) 3 23 49
  3. ROCK isoform regulation of myosin phosphatase and contractility in vascular smooth muscle cells. (PMID: 19131646) Wang Y. … Surks H.K. (Circ. Res. 2009) 3 4 23
  4. Localization of the gene coding for ROCK II/Rho kinase on human chromosome 2p24. (PMID: 9933571) Takahashi N. … Kaibuchi K. (Genomics 1999) 2 3 4
  5. ROCK2 allelic variants are not associated with pre-eclampsia susceptibility in the Finnish population. (PMID: 19435756) Peterson H. … Kivinen K. (Mol. Hum. Reprod. 2009) 3 23 49

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