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Aliases for ROCK2 Gene

Aliases for ROCK2 Gene

  • Rho-Associated, Coiled-Coil Containing Protein Kinase 2 2 3
  • Rho-Associated, Coiled-Coil-Containing Protein Kinase II 3 4
  • P164 ROCK-2 3 4
  • EC 2.7.11.1 4 63
  • ROCK-II 3 4
  • Rho-Associated, Coiled-Coil-Containing Protein Kinase 2 4
  • Rho Kinase 2 4
  • EC 2.7.11 63
  • KIAA0619 4

External Ids for ROCK2 Gene

Previous GeneCards Identifiers for ROCK2 Gene

  • GC02M011106
  • GC02M011371
  • GC02M011343
  • GC02M011272
  • GC02M011319

Summaries for ROCK2 Gene

Entrez Gene Summary for ROCK2 Gene

  • The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008]

GeneCards Summary for ROCK2 Gene

ROCK2 (Rho-Associated, Coiled-Coil Containing Protein Kinase 2) is a Protein Coding gene. Diseases associated with ROCK2 include penile disease. Among its related pathways are Signaling by GPCR and Regulation of actin cytoskeleton. GO annotations related to this gene include poly(A) RNA binding and protein kinase activity. An important paralog of this gene is STK38L.

UniProtKB/Swiss-Prot for ROCK2 Gene

  • Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.

Tocris Summary for ROCK2 Gene

  • Rho-kinases (aka Rho-associated coiled-coil kinases or ROCKs) are serine/threonine kinases activated by RhoA GTPases. They are modulators of processes involving cytoskeletal rearrangement such as focal adhesion formation, cell motility and tumor cell invasion.

No data available for Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for ROCK2 Gene

Genomics for ROCK2 Gene

Regulatory Elements for ROCK2 Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for ROCK2 Gene

Chromosome:
2
Start:
11,179,761 bp from pter
End:
11,348,330 bp from pter
Size:
168,570 bases
Orientation:
Minus strand

Genomic View for ROCK2 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for ROCK2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for ROCK2 Gene

Proteins for ROCK2 Gene

  • Protein details for ROCK2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    O75116-ROCK2_HUMAN
    Recommended name:
    Rho-associated protein kinase 2
    Protein Accession:
    O75116
    Secondary Accessions:
    • Q53QZ0
    • Q53SJ7
    • Q9UQN5

    Protein attributes for ROCK2 Gene

    Size:
    1388 amino acids
    Molecular mass:
    160900 Da
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    • Homodimer. Interacts with IRS1, RHOB and RHOC (By similarity). Interacts with RHOA (activated by GTP), PPP1R12A, CHORDC1, SORL1, EP300 and BRCA2. Interacts with NPM1 and this interaction enhances its activity. Interacts with RAF1 (By similarity).
    SequenceCaution:
    • Sequence=AAX93049.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; Sequence=BAA31594.2; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for ROCK2 Gene

neXtProt entry for ROCK2 Gene

Proteomics data for ROCK2 Gene at MOPED

Post-translational modifications for ROCK2 Gene

  • Cleaved by granzyme B during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing
  • Phosphorylation at Tyr-722 reduces its binding to RHOA and is crucial for focal adhesion dynamics. Dephosphorylation by PTPN11 stimulates its RHOA binding activity.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for ROCK2 Gene

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for ROCK2 (ROCK2)

Domains & Families for ROCK2 Gene

Suggested Antigen Peptide Sequences for ROCK2 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

O75116

UniProtKB/Swiss-Prot:

ROCK2_HUMAN :
  • An interaction between Thr-414 and Asp-48 is essential for kinase activity and dimerization.
  • Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
  • Contains 1 phorbol-ester/DAG-type zinc finger.
Domain:
  • An interaction between Thr-414 and Asp-48 is essential for kinase activity and dimerization.
  • Contains 1 AGC-kinase C-terminal domain.
  • Contains 1 PH domain.
  • Contains 1 protein kinase domain.
Family:
  • Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Similarity:
  • Contains 1 phorbol-ester/DAG-type zinc finger.
  • Contains 1 REM (Hr1) repeat.
genes like me logo Genes that share domains with ROCK2: view

Function for ROCK2 Gene

Molecular function for ROCK2 Gene

GENATLAS Biochemistry:
serine/threonine kinase ROCK2/rho kinase,isozyme of ROCK1,one the targets for the small GTPase Rho,putative mediator of the Rho-dependent signaling pathway
UniProtKB/Swiss-Prot CatalyticActivity:
ATP + a protein = ADP + a phosphoprotein
UniProtKB/Swiss-Prot EnzymeRegulation:
Activated by RHOA binding. Inhibited by Y-27632 (By similarity).
UniProtKB/Swiss-Prot Function:
Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.

Enzyme Numbers (IUBMB) for ROCK2 Gene

Gene Ontology (GO) - Molecular Function for ROCK2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004672 protein kinase activity --
GO:0004674 protein serine/threonine kinase activity IDA 16574662
GO:0005198 structural molecule activity NAS 9933571
GO:0005515 protein binding IPI 17015463
GO:0005524 ATP binding IEA --
genes like me logo Genes that share ontologies with ROCK2: view
genes like me logo Genes that share phenotypes with ROCK2: view

Animal Models for ROCK2 Gene

MGI Knock Outs for ROCK2:

Animal Model Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for ROCK2

In Situ Assay Products

Flow Cytometry Products

No data available for Transcription Factor Targets and HOMER Transcription for ROCK2 Gene

Localization for ROCK2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for ROCK2 Gene

Cytoplasm. Cell membrane; Peripheral membrane protein. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Cytoplasmic, and associated with actin microfilaments and the plasma membrane. {ECO:0000250}.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for ROCK2 Gene COMPARTMENTS Subcellular localization image for ROCK2 gene
Compartment Confidence
cytoskeleton 5
nucleus 5
cytosol 4
plasma membrane 3
extracellular 1

Gene Ontology (GO) - Cellular Components for ROCK2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005622 intracellular --
GO:0005634 nucleus IEA --
GO:0005813 centrosome IDA 17015463
GO:0005829 cytosol TAS --
GO:0005886 plasma membrane IEA --
genes like me logo Genes that share ontologies with ROCK2: view

Pathways & Interactions for ROCK2 Gene

genes like me logo Genes that share pathways with ROCK2: view

PCR Array Products

  • Pathway & Disease-focused RT² Profiler PCR Arrays

SIGNOR curated interactions for ROCK2 Gene

Activates:
Inactivates:

Gene Ontology (GO) - Biological Process for ROCK2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000910 cytokinesis NAS 9933571
GO:0001934 positive regulation of protein phosphorylation IMP 24065547
GO:0006468 protein phosphorylation IDA 18559669
GO:0006939 smooth muscle contraction TAS 12778124
GO:0007165 signal transduction --
genes like me logo Genes that share ontologies with ROCK2: view

Drugs & Compounds for ROCK2 Gene

(15) Drugs for ROCK2 Gene - From: HMDB, DGIdb, ApexBio, Tocris, and DrugBank

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Adenosine triphosphate Approved Nutra 0
(R)-TRANS-4-(1-AMINOETHYL)-N-(4-PYRIDYL) CYCLOHEXANECARBOXAMIDE Experimental Pharma Target 0
5-(1,4-DIAZEPAN-1-SULFONYL)ISOQUINOLINE Experimental Pharma Target 0
Guanosine triphosphate Experimental Pharma 0
Y-27632 dihydrochloride Pharma ROCK1 inhibitor, Selective p160ROCK inhibitor 0

(9) Additional Compounds for ROCK2 Gene - From: HMDB and Tocris

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
ADP
  • Adenosindiphosphorsaeure
  • Adenosine 5'-pyrophosphate
  • Adenosine diphosphate
  • Adenosine pyrophosphate
  • Adenosine-5'-diphosphate
Antagonist, Gating inhibitor 58-64-0
Diglycerides Group A
  • 1,2-Dimyristoyl-rac-glycerol
  • DAG(14:0/14:0)
  • DAG(28:0)
  • DG(14:0/14:0)
  • DG(28:0)
Diglycerides Group B
  • 1-Palmitoleoyl-2-arachidonyl-sn-glycerol
  • DAG(16:1/20:0)
  • DAG(16:1n7/20:0)
  • DAG(16:1w7/20:0)
  • DAG(36:1)
Diglycerides Group C
  • 1-Myristoyl-3-stearoyl-sn-glycerol
  • 1-Tetradecanoyl-3-octadecanoyl-sn-glycerol
  • DAG(14:0/0:0/18:0)
  • DAG(32:0)
  • DG(32:0)
Diglycerides Group D
  • 1,2-Didocosahexaenoyl-rac-glycerol
  • DAG(22:6/22:6)
  • DAG(22:6n3/22:6n3)
  • DAG(22:6w3/22:6w3)
  • DAG(44:12)
genes like me logo Genes that share compounds with ROCK2: view

Drug Products

Transcripts for ROCK2 Gene

Unigene Clusters for ROCK2 Gene

Rho-associated, coiled-coil containing protein kinase 2:
Representative Sequences:

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for ROCK2

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for ROCK2 Gene

No ASD Table

Relevant External Links for ROCK2 Gene

GeneLoc Exon Structure for
ROCK2
ECgene alternative splicing isoforms for
ROCK2

Expression for ROCK2 Gene

mRNA expression in normal human tissues for ROCK2 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for ROCK2 Gene

This gene is overexpressed in Peripheral blood mononuclear cells (18.4), Platelet (8.6), and Heart (7.7).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for ROCK2 Gene



SOURCE GeneReport for Unigene cluster for ROCK2 Gene Hs.681743

genes like me logo Genes that share expression patterns with ROCK2: view

Protein tissue co-expression partners for ROCK2 Gene

- Elite partner

Primer Products

In Situ Assay Products

No data available for mRNA differential expression in normal tissues and mRNA Expression by UniProt/SwissProt for ROCK2 Gene

Orthologs for ROCK2 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for ROCK2 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia ROCK2 35
  • 93.52 (n)
  • 97.77 (a)
ROCK2 36
  • 98 (a)
OneToOne
dog
(Canis familiaris)
Mammalia ROCK2 35
  • 92.17 (n)
  • 96.15 (a)
ROCK2 36
  • 97 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Rock2 35
  • 91.04 (n)
  • 96.69 (a)
Rock2 16
Rock2 36
  • 97 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia ROCK2 35
  • 99.83 (n)
  • 99.86 (a)
ROCK2 36
  • 100 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Rock2 35
  • 90.73 (n)
  • 96.76 (a)
oppossum
(Monodelphis domestica)
Mammalia ROCK2 36
  • 90 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia ROCK2 36
  • 88 (a)
OneToOne
chicken
(Gallus gallus)
Aves ROCK2 35
  • 83.16 (n)
  • 91.54 (a)
ROCK2 36
  • 85 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia ROCK2 36
  • 84 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia rock2 35
  • 75.39 (n)
  • 83.32 (a)
Str.15799 35
African clawed frog
(Xenopus laevis)
Amphibia rocka-A-prov 35
zebrafish
(Danio rerio)
Actinopterygii rock2 35
rock2a 35
  • 69.69 (n)
  • 76.79 (a)
ROCK2 (3 of 10) 36
  • 78 (a)
OneToMany
ROCK2 (4 of 10) 36
  • 79 (a)
OneToMany
ROCK2 (5 of 10) 36
  • 78 (a)
OneToMany
ROCK2 (6 of 10) 36
  • 81 (a)
OneToMany
ROCK2 (7 of 10) 36
  • 79 (a)
OneToMany
ROCK2 (8 of 10) 36
  • 73 (a)
OneToMany
ROCK2 (9 of 10) 36
  • 80 (a)
OneToMany
rock2a 36
  • 76 (a)
OneToMany
rock2b 36
  • 65 (a)
OneToMany
rock2b 36
  • 64 (a)
OneToMany
fruit fly
(Drosophila melanogaster)
Insecta rok 37
  • 43 (a)
rok 35
  • 49.79 (n)
  • 46.14 (a)
rok 36
  • 43 (a)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP000406 35
  • 48.97 (n)
  • 45.74 (a)
worm
(Caenorhabditis elegans)
Secernentea let-502 37
  • 33 (a)
let-502 35
  • 52.17 (n)
  • 43.6 (a)
let-502 36
  • 36 (a)
OneToOne
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes CBK1 36
  • 22 (a)
OneToMany
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 48 (a)
OneToOne
Species with no ortholog for ROCK2:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for ROCK2 Gene

ENSEMBL:
Gene Tree for ROCK2 (if available)
TreeFam:
Gene Tree for ROCK2 (if available)

Paralogs for ROCK2 Gene

genes like me logo Genes that share paralogs with ROCK2: view

Variants for ROCK2 Gene

Sequence variations from dbSNP and Humsavar for ROCK2 Gene

SNP ID Clin Chr 02 pos Sequence Context AA Info Type MAF
rs8996 -- 11,180,751(-) TTCTC(A/G)TTTTT utr-variant-3-prime
rs9349 -- 11,180,614(-) CTTAT(A/T)GGATT utr-variant-3-prime
rs372345 -- 11,199,916(+) CTACT(A/C/G/T)ACTGT intron-variant
rs372350 -- 11,199,921(+) TACTG(A/C/G/T)ACTAT intron-variant
rs373008 -- 11,200,053(+) AAAGT(C/T)GTTTT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for ROCK2 Gene

Variant ID Type Subtype PubMed ID
esv2592438 CNV Insertion 19546169
esv267549 CNV Insertion 20981092
esv272377 CNV Insertion 20981092
nsv526038 CNV Loss 19592680
nsv2607 CNV Loss 18451855
esv2658593 CNV Deletion 23128226
nsv436304 CNV Deletion 17901297
esv25473 CNV Loss 19812545
nsv498912 CNV Loss 21111241
nsv514052 CNV Loss 21397061
esv2543558 CNV Insertion 19546169
esv271091 CNV Insertion 20981092
esv272435 CNV Insertion 20981092
esv1339412 CNV Insertion 17803354
esv2453181 CNV Insertion 19546169
esv2662875 CNV Deletion 23128226

Variation tolerance for ROCK2 Gene

Residual Variation Intolerance Score: 17.93% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 9.61; 89.05% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for ROCK2 Gene

HapMap Linkage Disequilibrium report
ROCK2
Human Gene Mutation Database (HGMD)
ROCK2

PCR Panel Products

  • Copy Number PCR Panels: for ROCK2

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for ROCK2 Gene

Disorders for ROCK2 Gene

MalaCards: The human disease database

(1) MalaCards diseases for ROCK2 Gene - From: DISEASES

Disorder Aliases PubMed IDs
penile disease
  • penile diseases
- elite association
Search ROCK2 in MalaCards View complete list of genes associated with diseases

Relevant External Links for ROCK2

Genetic Association Database (GAD)
ROCK2
Human Genome Epidemiology (HuGE) Navigator
ROCK2
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
ROCK2
genes like me logo Genes that share disorders with ROCK2: view

No data available for UniProtKB/Swiss-Prot and Genatlas for ROCK2 Gene

Publications for ROCK2 Gene

  1. Increased leukocyte rho kinase (ROCK) activity and endothelial dysfunction in cigarette smokers. (PMID: 20139919) Hidaka T. … Higashi Y. (Hypertens. Res. 2010) 23 67
  2. Rho kinase-2 activation in human endothelial cells drives lysophosphatidic acid-mediated expression of cell adhesion molecules via NF-kappaB p65. (PMID: 20164172) Shimada H. … Rajagopalan L.E. (J. Biol. Chem. 2010) 23 67
  3. ROCK isoform regulation of myosin phosphatase and contractility in vascular smooth muscle cells. (PMID: 19131646) Wang Y. … Surks H.K. (Circ. Res. 2009) 23 67
  4. Proteomic identification of proteins translocated to membrane microdomains upon treatment of fibroblasts with the glycosphingolipid, C8-beta-D-lactosylceramide. (PMID: 19634142) Kim S.Y. … Pagano R.E. (Proteomics 2009) 23 67
  5. Inhibition of Rho kinases enhances the degradation of mutant huntingtin. (PMID: 19278999) Bauer P.O. … Nukina N. (J. Biol. Chem. 2009) 23 67

Products for ROCK2 Gene

Sources for ROCK2 Gene