Free for academic non-profit institutions. Other users need a Commercial license

Aliases for ROCK1 Gene

Aliases for ROCK1 Gene

  • Rho Associated Coiled-Coil Containing Protein Kinase 1 2 3
  • Rho-Associated, Coiled-Coil Containing Protein Kinase 1 3 5
  • Renal Carcinoma Antigen NY-REN-35 3 4
  • P160 ROCK-1 3 4
  • EC 2.7.11.1 4 63
  • P160ROCK 3 4
  • ROCK-I 3 4
  • Rho-Associated, Coiled-Coil-Containing Protein Kinase 1 4
  • Rho-Associated, Coiled-Coil-Containing Protein Kinase I 4
  • EC 2.7.11 63

External Ids for ROCK1 Gene

Previous GeneCards Identifiers for ROCK1 Gene

  • GC18M018388
  • GC18M018263
  • GC18M016785
  • GC18M016787
  • GC18M018529
  • GC18M015376

Summaries for ROCK1 Gene

Entrez Gene Summary for ROCK1 Gene

  • This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18. [provided by RefSeq, Aug 2015]

GeneCards Summary for ROCK1 Gene

ROCK1 (Rho Associated Coiled-Coil Containing Protein Kinase 1) is a Protein Coding gene. Diseases associated with ROCK1 include sry-negative 46,xx testicular disorder of sex development and pediatric osteosarcoma. Among its related pathways are Signaling by GPCR and Signaling by Rho GTPases. GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is CDC42BPA.

UniProtKB/Swiss-Prot for ROCK1 Gene

  • Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization.

Tocris Summary for ROCK1 Gene

  • Rho-kinases (aka Rho-associated coiled-coil kinases or ROCKs) are serine/threonine kinases activated by RhoA GTPases. They are modulators of processes involving cytoskeletal rearrangement such as focal adhesion formation, cell motility and tumor cell invasion.

Gene Wiki entry for ROCK1 Gene

No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for ROCK1 Gene

Genomics for ROCK1 Gene

Regulatory Elements for ROCK1 Gene

Promoters for ROCK1 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around ROCK1 on UCSC Golden Path with GeneCards custom track

Genomic Location for ROCK1 Gene

Chromosome:
18
Start:
20,946,906 bp from pter
End:
21,111,851 bp from pter
Size:
164,946 bases
Orientation:
Minus strand

Genomic View for ROCK1 Gene

Genes around ROCK1 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
ROCK1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for ROCK1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for ROCK1 Gene

Proteins for ROCK1 Gene

  • Protein details for ROCK1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q13464-ROCK1_HUMAN
    Recommended name:
    Rho-associated protein kinase 1
    Protein Accession:
    Q13464
    Secondary Accessions:
    • B0YJ91
    • Q2KHM4
    • Q59GZ4

    Protein attributes for ROCK1 Gene

    Size:
    1354 amino acids
    Molecular mass:
    158175 Da
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    • Homodimer. Interacts with RHOB, RHOC, MYLC2B and PTEN. Interacts with ITGB1BP1 (via N-terminus and PTB domain) (By similarity). Interacts with RHOA (activated by GTP), CHORDC1, DAPK3, GEM, JIP3, RHOE, PPP1R12A, PFN1, LIMK1, LIMK2 and TSG101. Interacts with FHOD1 in a Src-dependent manner.

    Three dimensional structures from OCA and Proteopedia for ROCK1 Gene

neXtProt entry for ROCK1 Gene

Proteomics data for ROCK1 Gene at MOPED

Post-translational modifications for ROCK1 Gene

  • Autophosphorylated on serine and threonine residues.
  • Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for ROCK1 Gene

ENSEMBL proteins:
REFSEQ proteins:

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for ROCK1 (ROCK1)

Domains & Families for ROCK1 Gene

Suggested Antigen Peptide Sequences for ROCK1 Gene

Graphical View of Domain Structure for InterPro Entry

Q13464

UniProtKB/Swiss-Prot:

ROCK1_HUMAN :
  • The C-terminal auto-inhibitory domain interferes with kinase activity. RHOA binding leads to a conformation change and activation of the kinase. Truncated ROCK1 is constitutively activated.
  • Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
  • Contains 1 phorbol-ester/DAG-type zinc finger.
Domain:
  • The C-terminal auto-inhibitory domain interferes with kinase activity. RHOA binding leads to a conformation change and activation of the kinase. Truncated ROCK1 is constitutively activated.
  • Contains 1 AGC-kinase C-terminal domain.
  • Contains 1 PH domain.
  • Contains 1 protein kinase domain.
Family:
  • Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Similarity:
  • Contains 1 phorbol-ester/DAG-type zinc finger.
  • Contains 1 REM (Hr1) repeat.
genes like me logo Genes that share domains with ROCK1: view

Function for ROCK1 Gene

Molecular function for ROCK1 Gene

UniProtKB/Swiss-Prot CatalyticActivity:
ATP + a protein = ADP + a phosphoprotein.
UniProtKB/Swiss-Prot EnzymeRegulation:
Activated by RHOA binding. Inhibited by Y-27632.
UniProtKB/Swiss-Prot Function:
Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization.

Enzyme Numbers (IUBMB) for ROCK1 Gene

Gene Ontology (GO) - Molecular Function for ROCK1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0017048 Rho GTPase binding IEA --
genes like me logo Genes that share ontologies with ROCK1: view
genes like me logo Genes that share phenotypes with ROCK1: view

Animal Models for ROCK1 Gene

MGI Knock Outs for ROCK1:

Animal Model Products

In Situ Assay Products

Flow Cytometry Products

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for ROCK1 Gene

Localization for ROCK1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for ROCK1 Gene

Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Golgi apparatus membrane; Peripheral membrane protein. Cell projection, bleb. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, ruffle. Note=Associated with the mother centriole and an intercentriolar linker. Colocalizes with ITGB1BP1 and ITGB1 at the cell membrane predominantly in lamellipodia and membrane ruffles, but also in retraction fibers. Localizes at the cell membrane in an ITGB1BP1-dependent manner (By similarity). A small proportion is associated with Golgi membranes. {ECO:0000250}.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for ROCK1 Gene COMPARTMENTS Subcellular localization image for ROCK1 gene
Compartment Confidence
cytoskeleton 5
cytosol 5
plasma membrane 5
golgi apparatus 4
nucleus 3
mitochondrion 2
extracellular 1

Gene Ontology (GO) - Cellular Components for ROCK1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001726 ruffle IEA,ISS --
genes like me logo Genes that share ontologies with ROCK1: view

Pathways & Interactions for ROCK1 Gene

genes like me logo Genes that share pathways with ROCK1: view

Pathways by source for ROCK1 Gene

SIGNOR curated interactions for ROCK1 Gene

Gene Ontology (GO) - Biological Process for ROCK1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006915 apoptotic process TAS --
GO:0006921 cellular component disassembly involved in execution phase of apoptosis TAS --
GO:0007159 leukocyte cell-cell adhesion IDA 12082081
GO:0007165 signal transduction IEA,TAS 10436159
GO:0007249 I-kappaB kinase/NF-kappaB signaling IMP 25816133
genes like me logo Genes that share ontologies with ROCK1: view

Drugs & Compounds for ROCK1 Gene

(19) Drugs for ROCK1 Gene - From: HMDB, DGIdb, DrugBank, ApexBio, Tocris, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Adenosine triphosphate Approved Nutra 0
(R)-TRANS-4-(1-AMINOETHYL)-N-(4-PYRIDYL) CYCLOHEXANECARBOXAMIDE Experimental Pharma Target 0
(S)-2-METHYL-1-[(4-METHYL-5-ISOQUINOLINE)SULFONYL]-HOMOPIPERAZINE Experimental Pharma Target 0
5-(1,4-DIAZEPAN-1-SULFONYL)ISOQUINOLINE Experimental Pharma Target 0
HYDROXYFASUDIL Experimental Pharma Target, inhibitor 0

(12) Additional Compounds for ROCK1 Gene - From: Novoseek, HMDB, and Tocris

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
ADP
  • Adenosindiphosphorsaeure
  • Adenosine 5'-pyrophosphate
  • Adenosine diphosphate
  • Adenosine pyrophosphate
  • Adenosine-5'-diphosphate
Full agonist, Agonist 58-64-0
Diglycerides Group A
  • 1,2-Dimyristoyl-rac-glycerol
  • DAG(14:0/14:0)
  • DAG(28:0)
  • DG(14:0/14:0)
  • DG(28:0)
Diglycerides Group B
  • 1-Palmitoleoyl-2-arachidonyl-sn-glycerol
  • DAG(16:1/20:0)
  • DAG(16:1n7/20:0)
  • DAG(16:1w7/20:0)
  • DAG(36:1)
Diglycerides Group C
  • 1-Myristoyl-3-stearoyl-sn-glycerol
  • 1-Tetradecanoyl-3-octadecanoyl-sn-glycerol
  • DAG(14:0/0:0/18:0)
  • DAG(32:0)
  • DG(32:0)
Diglycerides Group D
  • 1,2-Didocosahexaenoyl-rac-glycerol
  • DAG(22:6/22:6)
  • DAG(22:6n3/22:6n3)
  • DAG(22:6w3/22:6w3)
  • DAG(44:12)
genes like me logo Genes that share compounds with ROCK1: view

Drug Products

Transcripts for ROCK1 Gene

Unigene Clusters for ROCK1 Gene

Rho-associated, coiled-coil containing protein kinase 1:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for ROCK1 Gene

No ASD Table

Relevant External Links for ROCK1 Gene

GeneLoc Exon Structure for
ROCK1
ECgene alternative splicing isoforms for
ROCK1

Expression for ROCK1 Gene

mRNA expression in normal human tissues for ROCK1 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for ROCK1 Gene

This gene is overexpressed in Artery - Tibial (x4.0).

Protein differential expression in normal tissues from HIPED for ROCK1 Gene

This gene is overexpressed in Peripheral blood mononuclear cells (15.3) and Lymph node (13.7).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for ROCK1 Gene



SOURCE GeneReport for Unigene cluster for ROCK1 Gene Hs.306307

mRNA Expression by UniProt/SwissProt for ROCK1 Gene

Q13464-ROCK1_HUMAN
Tissue specificity: Detected in blood platelets.
genes like me logo Genes that share expression patterns with ROCK1: view

Protein tissue co-expression partners for ROCK1 Gene

- Elite partner

Primer Products

In Situ Assay Products

Orthologs for ROCK1 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for ROCK1 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia ROCK1 35
  • 99.75 (n)
  • 99.93 (a)
ROCK1 36
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia ROCK1 36
  • 93 (a)
OneToOne
ROCK1 35
  • 93.82 (n)
  • 97.07 (a)
dog
(Canis familiaris)
Mammalia ROCK1 35
  • 94.68 (n)
  • 97.64 (a)
ROCK1 36
  • 96 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Rock1 16
Rock1 35
  • 92.05 (n)
  • 96.6 (a)
Rock1 36
  • 97 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia ROCK1 36
  • 92 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia ROCK1 36
  • 98 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Rock1 35
  • 91.73 (n)
  • 95.86 (a)
chicken
(Gallus gallus)
Aves ROCK1 35
  • 81.69 (n)
  • 87.51 (a)
ROCK1 36
  • 87 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia ROCK1 36
  • 87 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia rock1 35
  • 76.8 (n)
  • 82.77 (a)
zebrafish
(Danio rerio)
Actinopterygii rock1 36
  • 75 (a)
OneToOne
rock1 35
  • 69.04 (n)
  • 75.98 (a)
fruit fly
(Drosophila melanogaster)
Insecta rok 37
  • 43 (a)
worm
(Caenorhabditis elegans)
Secernentea let-502 37
  • 34 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes CBK1 36
  • 22 (a)
OneToMany
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.501 35
Species with no ortholog for ROCK1:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for ROCK1 Gene

ENSEMBL:
Gene Tree for ROCK1 (if available)
TreeFam:
Gene Tree for ROCK1 (if available)

Paralogs for ROCK1 Gene

Paralogs for ROCK1 Gene

Pseudogenes.org Pseudogenes for ROCK1 Gene

genes like me logo Genes that share paralogs with ROCK1: view

Variants for ROCK1 Gene

Sequence variations from dbSNP and Humsavar for ROCK1 Gene

SNP ID Clin Chr 18 pos Sequence Context AA Info Type
rs55811609 - 21,049,183(+) ATTTG(C/T)TGAGA reference, missense
rs45562542 - 20,984,522(+) GCAGG(A/C/G)TTAGA reference, missense
rs35881519 - 20,966,935(+) ATCAG(C/G/T)TTCAT reference, missense
VAR_041058 A lung neuroendocrine carcinoma sample
VAR_041059 -

Structural Variations from Database of Genomic Variants (DGV) for ROCK1 Gene

Variant ID Type Subtype PubMed ID
nsv833594 CNV Gain+Loss 17160897
esv270844 CNV Insertion 20981092
esv273128 CNV Insertion 20981092

Variation tolerance for ROCK1 Gene

Residual Variation Intolerance Score: 8.32% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 1.86; 34.83% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for ROCK1 Gene

HapMap Linkage Disequilibrium report
ROCK1
Human Gene Mutation Database (HGMD)
ROCK1

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for ROCK1 Gene

Disorders for ROCK1 Gene

MalaCards: The human disease database

(4) MalaCards diseases for ROCK1 Gene - From: DISEASES and GeneCards

Disorder Aliases PubMed IDs
sry-negative 46,xx testicular disorder of sex development
  • sry-negative xx male syndrome
pediatric osteosarcoma
  • childhood osteosarcoma
46,xx testicular disorder of sex development
  • 46,xx testicular dsd
tetralogy of fallot
  • tetrology of fallot
- elite association - COSMIC cancer census association via MalaCards
Search ROCK1 in MalaCards View complete list of genes associated with diseases

Relevant External Links for ROCK1

Genetic Association Database (GAD)
ROCK1
Human Genome Epidemiology (HuGE) Navigator
ROCK1
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
ROCK1
genes like me logo Genes that share disorders with ROCK1: view

No data available for UniProtKB/Swiss-Prot and Genatlas for ROCK1 Gene

Publications for ROCK1 Gene

  1. The small GTP-binding protein Rho binds to and activates a 160 kDa Ser/Thr protein kinase homologous to myotonic dystrophy kinase. (PMID: 8617235) Ishizaki T. … Narumiya S. (EMBO J. 1996) 2 3 4 67
  2. ROCK isoform regulation of myosin phosphatase and contractility in vascular smooth muscle cells. (PMID: 19131646) Wang Y. … Surks H.K. (Circ. Res. 2009) 3 23
  3. Rho kinase-1 mediates cardiac fibrosis by regulating fibroblast precursor cell differentiation. (PMID: 19406912) Haudek S.B. … Entman M.L. (Cardiovasc. Res. 2009) 3 23
  4. PDK1 regulates cancer cell motility by antagonising inhibition of ROCK1 by RhoE. (PMID: 18204440) Pinner S. … Sahai E. (Nat. Cell Biol. 2008) 3 23
  5. Phosphorylation of profilin by ROCK1 regulates polyglutamine aggregation. (PMID: 18573880) Shao J. … Diamond M.I. (Mol. Cell. Biol. 2008) 3 23

Products for ROCK1 Gene

Sources for ROCK1 Gene

Back to Top

Content