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Aliases for ROCK1 Gene

Aliases for ROCK1 Gene

  • Rho-Associated, Coiled-Coil Containing Protein Kinase 1 2 3
  • Rho-Associated, Coiled-Coil-Containing Protein Kinase 1 3 4
  • Rho-Associated, Coiled-Coil-Containing Protein Kinase I 3 4
  • Renal Carcinoma Antigen NY-REN-35 3 4
  • P160 ROCK-1 3 4
  • EC 2.7.11.1 4 63
  • P160ROCK 3 4
  • ROCK-I 3 4
  • Rho-Associated Protein Kinase 1 3
  • Rho Kinase 3
  • P160-ROCK 3
  • EC 2.7.11 63

External Ids for ROCK1 Gene

Summaries for ROCK1 Gene

Entrez Gene Summary for ROCK1 Gene

  • This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq, Jul 2008]

GeneCards Summary for ROCK1 Gene

ROCK1 (Rho-Associated, Coiled-Coil Containing Protein Kinase 1) is a Protein Coding gene. Diseases associated with ROCK1 include sry-negative 46,xx testicular disorder of sex development and 46,xx testicular disorder of sex development. Among its related pathways are RhoGDI Pathway and Signaling by GPCR. GO annotations related to this gene include protein serine/threonine kinase activity and protein kinase activity. An important paralog of this gene is STK38L.

UniProtKB/Swiss-Prot for ROCK1 Gene

  • Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization.

Tocris Summary for ROCK1 Gene

  • Rho-kinases (aka Rho-associated coiled-coil kinases or ROCKs) are serine/threonine kinases that are activated by RhoA GTPases, and are key modulators of processes involving cytoskeletal rearrangement such focal adhesion formation, cell motility and tumor cell invasion. There are two isoforms of ROCK, ROCKI (aka ROKbeta or p160ROCK) and ROCKII (aka ROKalpha), which share a high degree of structural identity in their kinase domains (92%). The two Rho-kinase isoforms have different patterns of tissue expression; ROCKII expression is highest in the brain and in muscle whereas ROCKI has a ubiquitous tissue distribution. Subcellularly, ROCKII is found in the cytosol and localizes to the cleavage furrow during cytokinesis. ROCKI is thought to colocalize with the centrosomes. ROCKs phosphorylate a number of proteins involved in actin filament assembly and contraction (e.g. LIM Kinases, MLCK, ERM proteins). Other downstream targets of the Rho kinases include signal transduction molecules such as IRS-1 and PI 3-K/AKT.

Gene Wiki entry for ROCK1 Gene

No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for ROCK1 Gene

Genomics for ROCK1 Gene

Genomic Location for ROCK1 Gene

Start:
20,946,906 bp from pter
End:
21,111,851 bp from pter
Size:
164,946 bases
Orientation:
Minus strand

Genomic View for ROCK1 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for ROCK1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for ROCK1 Gene

Regulatory Elements for ROCK1 Gene

Proteins for ROCK1 Gene

  • Protein details for ROCK1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q13464-ROCK1_HUMAN
    Recommended name:
    Rho-associated protein kinase 1
    Protein Accession:
    Q13464
    Secondary Accessions:
    • B0YJ91
    • Q2KHM4
    • Q59GZ4

    Protein attributes for ROCK1 Gene

    Size:
    1354 amino acids
    Molecular mass:
    158175 Da
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    • Homodimer. Interacts with RHOB, RHOC, MYLC2B and PTEN. Interacts with ITGB1BP1 (via N-terminus and PTB domain) (By similarity). Interacts with RHOA (activated by GTP), CHORDC1, DAPK3, GEM, JIP3, RHOE, PPP1R12A, PFN1, LIMK1, LIMK2 and TSG101. Interacts with FHOD1 in a Src-dependent manner.

    Three dimensional structures from OCA and Proteopedia for ROCK1 Gene

neXtProt entry for ROCK1 Gene

Proteomics data for ROCK1 Gene at MOPED

Post-translational modifications for ROCK1 Gene

  • Autophosphorylated on serine and threonine residues.
  • Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for ROCK1 Gene

ENSEMBL proteins:
Reactome Protein details:
REFSEQ proteins:

Domains for ROCK1 Gene

Gene Families for ROCK1 Gene

Suggested Antigen Peptide Sequences for ROCK1 Gene

Graphical View of Domain Structure for InterPro Entry

Q13464

UniProtKB/Swiss-Prot:

ROCK1_HUMAN
Domain:
  • The C-terminal auto-inhibitory domain interferes with kinase activity. RHOA binding leads to a conformation change and activation of the kinase. Truncated ROCK1 is constitutively activated:
    • Q13464
  • Contains 1 AGC-kinase C-terminal domain.:
    • Q13464
  • Contains 1 PH domain.:
    • Q13464
  • Contains 1 protein kinase domain.:
    • Q13464
Family:
  • Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.:
    • Q13464
Similarity:
  • Contains 1 phorbol-ester/DAG-type zinc finger.:
    • Q13464
  • Contains 1 REM (Hr1) repeat.:
    • Q13464
genes like me logo Genes that share domains with ROCK1: view

Function for ROCK1 Gene

Molecular function for ROCK1 Gene

UniProtKB/Swiss-Prot CatalyticActivity: ATP + a protein = ADP + a phosphoprotein
UniProtKB/Swiss-Prot EnzymeRegulation: Activated by RHOA binding. Inhibited by Y-27632
UniProtKB/Swiss-Prot Function: Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization.

Enzyme Numbers (IUBMB) for ROCK1 Gene

Gene Ontology (GO) - Molecular Function for ROCK1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004672 protein kinase activity TAS 10436159
GO:0004674 protein serine/threonine kinase activity IDA 10652353
GO:0004713 protein tyrosine kinase activity --
GO:0005515 protein binding IPI 10652353
GO:0005524 ATP binding IEA --
genes like me logo Genes that share ontologies with ROCK1: view
genes like me logo Genes that share phenotypes with ROCK1: view

Animal Models for ROCK1 Gene

MGI Knock Outs for ROCK1:

miRNA for ROCK1 Gene

No data available for Transcription Factor Targeting and HOMER Transcription for ROCK1 Gene

Localization for ROCK1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for ROCK1 Gene

Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Golgi apparatus membrane; Peripheral membrane protein. Cell projection, bleb. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, ruffle. Note=Associated with the mother centriole and an intercentriolar linker. Colocalizes with ITGB1BP1 and ITGB1 at the cell membrane predominantly in lamellipodia and membrane ruffles, but also in retraction fibers. Localizes at the cell membrane in an ITGB1BP1-dependent manner (By similarity). A small proportion is associated with Golgi membranes. {ECO:0000250}.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for ROCK1 Gene COMPARTMENTS Subcellular localization image for ROCK1 gene
Compartment Confidence
golgi apparatus 5
cytosol 4
cytoskeleton 2
extracellular 2
nucleus 2
endosome 1
mitochondrion 1
plasma membrane 1

Gene Ontology (GO) - Cellular Components for ROCK1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000139 Golgi membrane IEA --
GO:0001726 ruffle ISS --
GO:0005794 Golgi apparatus --
GO:0005814 centriole IEA --
GO:0005829 cytosol TAS --
genes like me logo Genes that share ontologies with ROCK1: view

Pathways for ROCK1 Gene

genes like me logo Genes that share pathways with ROCK1: view

Gene Ontology (GO) - Biological Process for ROCK1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003383 apical constriction IEA --
GO:0006468 protein phosphorylation IEA --
GO:0006915 apoptotic process TAS --
GO:0006921 cellular component disassembly involved in execution phase of apoptosis TAS --
GO:0006939 smooth muscle contraction TAS 12778124
genes like me logo Genes that share ontologies with ROCK1: view

Compounds for ROCK1 Gene

(9) ApexBio Compounds for ROCK1 Gene

Compound Action Cas Number
chroman 1 highly potent and selective ROCK II inhibitor. [1273579-40-0]
Fasudil (HA-1077) HCl Cyclic nucleotide-dependent protein kinase inhibitor and Rho-associated kinase inhibitor [105628-07-7]
GSK429286A Selective Rho-kinase inhibitor [864082-47-3]
H-1152 more specific, stronger and membrane-permeable inhibitor of Rho-kinase [451462-58-1]
Hydroxyfasudil potent Rho-kinase inhibitor [105628-72-6]
Narciclasine modulates the Rho/ROCK/LIM kinase/cofilin pathway [29477-83-6]
RKI-1447 potent small molecule inhibitor of ROCK1 and ROCK2. [1342278-01-6]
SLx-2119 Selective ROCK2 inhibitor [911417-87-3]
Y-27632 dihydrochloride p160ROCK inhibitor [146986-50-7]

(5) Tocris Compounds for ROCK1 Gene

Compound Action Cas Number
Fasudil hydrochloride Inhibitor of cyclic nucleotide dependent- and Rho-kinases [103745-39-7]
GSK 269962 Potent and selective ROCK inhibitor [850664-21-0]
H 1152 dihydrochloride Selective Rho-kinase (ROCK) inhibitor [451462-58-1]
SB 772077B dihydrochloride Potent Rho-kinase inhibitor; vasodilator [607373-46-6]
Y-27632 dihydrochloride Selective p160ROCK inhibitor [146986-50-7]

(846) HMDB Compounds for ROCK1 Gene

Compound Synonyms Cas Number PubMed IDs
Adenosine triphosphate
  • 5'-(tetrahydrogen triphosphate) Adenosine
56-65-5
ADP
  • adenosindiphosphorsaeure
58-64-0
DG(14:0/14:0/0:0)
  • DAG(14:0/14:0)
Not Available
DG(14:0/14:1(9Z)/0:0)
  • DAG(14:0/14:1)
Not Available
DG(14:0/15:0/0:0)
  • Diacylglycerol(29:0)
Not Available

(7) Novoseek inferred chemical compound relationships for ROCK1 Gene

Compound -log(P) Hits PubMed IDs
eipa 50 1
threonine 49 4
serine 41.4 3
fmlp 27.4 1
gtp 22.4 1
genes like me logo Genes that share compounds with ROCK1: view

Transcripts for ROCK1 Gene

Unigene Clusters for ROCK1 Gene

Rho-associated, coiled-coil containing protein kinase 1:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for ROCK1 Gene

No ASD Table

Relevant External Links for ROCK1 Gene

GeneLoc Exon Structure for
ROCK1
ECgene alternative splicing isoforms for
ROCK1

Expression for ROCK1 Gene

mRNA expression in normal human tissues for ROCK1 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for ROCK1 Gene

This gene is overexpressed in Artery - Tibial (4.0).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for ROCK1 Gene

SOURCE GeneReport for Unigene cluster for ROCK1 Gene Hs.306307

mRNA Expression by UniProt/SwissProt for ROCK1 Gene

Q13464-ROCK1_HUMAN
Tissue specificity: Detected in blood platelets.
genes like me logo Genes that share expressions with ROCK1: view

Orthologs for ROCK1 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for ROCK1 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia ROCK1 35
  • 99.75 (n)
  • 99.93 (a)
ROCK1 36
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia ROCK1 35
  • 93.82 (n)
  • 97.07 (a)
ROCK1 36
  • 93 (a)
OneToOne
dog
(Canis familiaris)
Mammalia ROCK1 35
  • 94.68 (n)
  • 97.64 (a)
ROCK1 36
  • 96 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Rock1 35
  • 92.05 (n)
  • 96.6 (a)
Rock1 16
Rock1 36
  • 97 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia ROCK1 36
  • 92 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia ROCK1 36
  • 98 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Rock1 35
  • 91.73 (n)
  • 95.86 (a)
chicken
(Gallus gallus)
Aves ROCK1 35
  • 81.69 (n)
  • 87.51 (a)
ROCK1 36
  • 87 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia ROCK1 36
  • 87 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia rock1 35
  • 76.8 (n)
  • 82.77 (a)
zebrafish
(Danio rerio)
Actinopterygii rock1 35
  • 69.04 (n)
  • 75.98 (a)
rock1 36
  • 75 (a)
OneToOne
fruit fly
(Drosophila melanogaster)
Insecta rok 37
  • 43 (a)
worm
(Caenorhabditis elegans)
Secernentea let-502 37
  • 34 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes CBK1 36
  • 22 (a)
OneToMany
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.501 35
Species with no ortholog for ROCK1:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for ROCK1 Gene

ENSEMBL:
Gene Tree for ROCK1 (if available)
TreeFam:
Gene Tree for ROCK1 (if available)

Paralogs for ROCK1 Gene

Pseudogenes.org Pseudogenes for ROCK1 Gene

genes like me logo Genes that share paralogs with ROCK1: view

Variants for ROCK1 Gene

Sequence variations from dbSNP and Humsavar for ROCK1 Gene

SNP ID Clin Chr 18 pos Sequence Context AA Info Type MAF
rs12372 -- 20,949,876(-) GATGG(A/G)TATCT utr-variant-3-prime
rs12734 -- 20,951,374(+) CACTC(A/G)GTCAC utr-variant-3-prime
rs14462 -- 20,950,043(+) TGATA(C/G)ACATT utr-variant-3-prime
rs167029 -- 21,017,255(-) gagat(C/T)gcgct intron-variant
rs167030 -- 21,018,341(-) TGTTG(A/G)CCAGG intron-variant

Structural Variations from Database of Genomic Variants (DGV) for ROCK1 Gene

Variant ID Type Subtype PubMed ID
nsv833594 CNV Gain+Loss 17160897
esv270844 CNV Insertion 20981092
esv273128 CNV Insertion 20981092

Relevant External Links for ROCK1 Gene

HapMap Linkage Disequilibrium report
ROCK1
Human Gene Mutation Database (HGMD)
ROCK1

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for ROCK1 Gene

Disorders for ROCK1 Gene

(5) Novoseek inferred disease relationships for ROCK1 Gene

Disease -log(P) Hits PubMed IDs
metastasis 21.1 4
ovarian cancer 0 6
tumors 0 1
cancer 0 3
heart failure 0 3

Relevant External Links for ROCK1

Genetic Association Database (GAD)
ROCK1
Human Genome Epidemiology (HuGE) Navigator
ROCK1
genes like me logo Genes that share disorders with ROCK1: view

No data available for UniProtKB/Swiss-Prot for ROCK1 Gene

Publications for ROCK1 Gene

  1. Different molecular mechanisms for Rho family GTPase-dependent, Ca2+- independent contraction of smooth muscle. (PMID: 9722579) Van Eyk J.E. … Mak A.S. (J. Biol. Chem. 1998) 3 4 23
  2. Phosphorylation of profilin by ROCK1 regulates polyglutamine aggregation. (PMID: 18573880) Shao J. … Diamond M.I. (Mol. Cell. Biol. 2008) 3 4 23
  3. The small GTP-binding protein Rho binds to and activates a 160 kDa Ser/Thr protein kinase homologous to myotonic dystrophy kinase. (PMID: 8617235) Ishizaki T. … Narumiya S. (EMBO J. 1996) 2 3 4
  4. ROCK isoform regulation of myosin phosphatase and contractility in vascular smooth muscle cells. (PMID: 19131646) Wang Y. … Surks H.K. (Circ. Res. 2009) 3 4 23
  5. Signaling from Rho to the actin cytoskeleton through protein kinases ROCK and LIM-kinase. (PMID: 10436159) Maekawa M. … Narumiya S. (Science 1999) 3 4

Products for ROCK1 Gene

Sources for ROCK1 Gene

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