Set Analyses:
Advanced Search

Advanced Search

 
Search By
Section (entire)
for


 
or upload a file of gene symbols


Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

RNF216 Gene

protein-coding   GIFtS: 55
GCID: GC07M005661

Ring Finger Protein 216

  See related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

Aliases
Ring Finger Protein 2161 2 3     UBCE7IP12 3
TRIAD32 3 5     CAHH2
ZIN2 3 5     U7I12
Triad Domain-Containing Protein 32 3     E3 Ubiquitin-Protein Ligase RNF2162
Ubiquitin-Conjugating Enzyme 7-Interacting Protein 12 3     EC 6.3.2.-3
Zinc Finger Protein Inhibiting NF-Kappa-B2 3     

External Ids:    HGNC: 216981   Entrez Gene: 544762   Ensembl: ENSG000000112757   OMIM: 6099485   UniProtKB: Q9NWF93   

Export aliases for RNF216 gene to outside databases

Previous GC identifer: GC07M005627


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for RNF216 Gene:
This gene encodes a cytoplasmic protein which specifically colocalizes and interacts with the serine/threonine
protein kinase, receptor-interacting protein (RIP). Zinc finger domains of the encoded protein are required for
its interaction with RIP and for inhibition of TNF- and IL1-induced NF-kappa B activation pathways. The encoded
protein may also function as an E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating
enzymes and transfers it to substrates. Several alternatively spliced transcript variants have been described for
this locus but the full-length natures of only some are known. (provided by RefSeq, Jul 2008)

GeneCards Summary for RNF216 Gene: 
RNF216 (ring finger protein 216) is a protein-coding gene. Diseases associated with RNF216 include obesity, and gastric cancer. GO annotations related to this gene include zinc ion binding and ligase activity.

UniProtKB/Swiss-Prot: RN216_HUMAN, Q9NWF9
Function: Isoform 1 acts as an E3 ubiquitin ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating
enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Promotes degradation
of TRAF3, TLR4 and TLR9. Contributes to the regulation of antiviral responses. Down-regulates activation of
NF-kappa-B, IRF3 activation and IFNB production. Isoform 3/ZIN inhibits TNF and IL-1 mediated activation of
NF-kappa-B. Promotes TNF and RIP mediated apoptosis

Gene Wiki entry for RNF216 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 73), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000007.13  NT_007819.17  NC_018918.2  NT_079592.2  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the RNF216 gene promoter:
         FOXF2   MAZR   NF-YA   CBF-C   CBF-A   CBF-B   CP1A   CP1C   NF-Y   CBF(2)   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 6): RNF216 promoter sequence
   Search SABiosciences Chromatin IP Primers for RNF216

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat RNF216


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 7p22.1   Ensembl cytogenetic band:  7p22.1   HGNC cytogenetic band: 7p22.1

RNF216 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
RNF216 gene location

GeneLoc information about chromosome 7         GeneLoc Exon Structure

GeneLoc location for GC07M005661:  view genomic region     (about GC identifiers)

Start:
5,659,672 bp from pter      End:
5,821,370 bp from pter
Size:
161,699 bases      Orientation:
minus strand

1 alternative location:
Chr7-,CRA_TCAG 5,706,017-5,867,706     

(According to UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MAXQB RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Ontologies according to Gene Ontology Consortium 01 Oct 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
About This Section

UniProtKB/Swiss-Prot: RN216_HUMAN, Q9NWF9 (See protein sequence)
Recommended Name: E3 ubiquitin-protein ligase RNF216  
Size: 866 amino acids; 99406 Da
Subunit: Interacts with UBE2L3 and to some extent with UBE2L6. Interacts with TRAF3, TLR3, TLR4, TLR5 and TLR9.
Isoform 3/ZIN binds RIPK1 and HIV VIF
Subcellular location: Cytoplasm
Sequence caution: Sequence=BAA91422.1; Type=Erroneous initiation; Note=Translation N-terminally extended;
Secondary accessions: Q6Y691 Q75ML7 Q7Z2H7 Q7Z7C1 Q8NHW7 Q9NYT1
Alternative splicing: 3 isoforms:  Q9NWF9-2   Q9NWF9-1   Q9NWF9-3   (4 different alternatively spliced mRNAs code for this protein isoform)

Explore the universe of human proteins at neXtProt for RNF216: NX_Q9NWF9

Explore proteomics data for RNF216 at MOPED 

Post-translational modifications:

  • UniProtKB: Auto-ubiquitinated
  • View modification sites using PhosphoSitePlus
  • View neXtProt modification sites for NX_Q9NWF9

  • RNF216 Protein expression data from MOPED1, PaxDb2 and MAXQB3 :    About this image 

    RNF216 Protein Expression
    REFSEQ proteins (2 alternative transcripts): 
    NP_996994.1  NP_996999.1  

    ENSEMBL proteins: 
     ENSP00000404602   ENSP00000374552   ENSP00000374550   ENSP00000409837   ENSP00000396829  

    Human Recombinant Protein Products for RNF216: 
    Browse Purified and Recombinant Proteins at EMD Millipore
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse OriGene full length recombinant human proteins expressed in human HEK293 cells
    OriGene Protein Over-expression Lysate for RNF216
    OriGene Custom MassSpec 
    OriGene Custom Protein Services for RNF216
    GenScript Custom Purified and Recombinant Proteins Services for RNF216
    Novus Biologicals RNF216 Lysate
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates 
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for RNF216 

    Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IDA--
    GO:0005730NOT nucleolus IDA--
    GO:0005737cytoplasm IDA--

    RNF216 for ontologies           About GeneDecksing



    RNF216 Antibody Products: 
    Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse R&D Systems for Antibodies
    OriGene Antibodies for RNF216
    OriGene Custom Antibody Services for RNF216
    GenScript Custom Superior Antibodies Services for RNF216
    Novus Biologicals RNF216 Antibodies
    Abcam antibodies for RNF216
    Cloud-Clone Corp. Antibodies for RNF216 
    ThermoFisher Antibodies for RNF216
    LSBio Antibodies in human, mouse, rat for RNF216 

    Assay Products for RNF216: 
    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for RNF216
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for RNF216
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for RNF216 
    Cloud-Clone Corp. CLIAs for RNF216


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section
    HGNC Gene Families: 
    RNF: RING-type (C3HC4) zinc fingers

    1 InterPro protein domain:
     IPR002867 Znf_C6HC

    Graphical View of Domain Structure for InterPro Entry Q9NWF9

    ProtoNet protein and cluster: Q9NWF9

    2 Blocks protein domains:
    IPB001841 Zn-finger
    IPB002867 Zn-finger


    UniProtKB/Swiss-Prot: RN216_HUMAN, Q9NWF9
    Domain: The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme (By similarity)
    Similarity: Contains 1 IBR-type zinc finger
    Similarity: Contains 2 RING-type zinc fingers


    RNF216 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, Vector BioLabs, and Sirion Biotech, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene.)
    About This Section

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: RN216_HUMAN, Q9NWF9
    Function: Isoform 1 acts as an E3 ubiquitin ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating
    enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Promotes degradation
    of TRAF3, TLR4 and TLR9. Contributes to the regulation of antiviral responses. Down-regulates activation of
    NF-kappa-B, IRF3 activation and IFNB production. Isoform 3/ZIN inhibits TNF and IL-1 mediated activation of
    NF-kappa-B. Promotes TNF and RIP mediated apoptosis

         Enzyme Number (IUBMB): EC 6.3.2.-1

         Gene Ontology (GO): 3 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515protein binding IPI12600646
    GO:0008270zinc ion binding IEA--
    GO:0016874ligase activity IEA--
         
    RNF216 for ontologies           About GeneDecksing


    Phenotypes:
         1 GenomeRNAi human phenotype for RNF216:
     Decreased POU5F1-GFP protein e 

    Animal Models:
       inGenious Targeting Laboratory - Custom generated mouse model solutions for RNF216 
       inGenious Targeting Laboratory - Custom generated inducible mouse model solutions for RNF216

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for RNF216 
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for RNF216 

    miRNA
    Products:
        
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat RNF216
    8/54 QIAGEN miScript miRNA Assays for microRNAs that regulate RNF216 (see all 54):
    hsa-miR-520e hsa-miR-302d hsa-miR-300 hsa-miR-4328 hsa-miR-199a-3p hsa-miR-301a hsa-miR-519a hsa-miR-766
    SwitchGear 3'UTR luciferase reporter plasmidRNF216 3' UTR sequence
    Inhib. RNA
    Products:
        
    Browse for Gene Knock-down Tools from EMD Millipore
    OriGene RNAi products in human, mouse, rat for RNF216
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat RNF216

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for RNF216
    Sirion Biotech Customized adenovirus for overexpression of RNF216

    Clone
    Products:
         
    Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
    OriGene clones in human, mouse for RNF216 (see all 12)
    OriGene ORF clones in mouse, rat for RNF216
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): RNF216 (NM_207111)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for RNF216
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RNF216
    Sirion Biotech Customized lentivirus for stable overexpression of RNF216 
                         Customized lentivirus expression plasmids for stable overexpression of RNF216 

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for RNF216
    Search LifeMap BioReagents cell lines for RNF216
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RNF216


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene).
    About This Section

    SuperPaths for RNF216 About                                                                                                See pathways by source

    SuperPathContained pathways About
    1Gastric cancer network 1
    Gastric cancer network 1
    2TNF-alpha/NF-kB Signaling Pathway
    TNF-alpha/NF-kB Signaling Pathway


    2 BioSystems Pathways for RNF216
        Gastric cancer network 1
    TNF-alpha/NF-kB Signaling Pathway


    UniProtKB/Swiss-Prot: RN216_HUMAN, Q9NWF9
    Pathway: Protein modification; protein ubiquitination

    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for RNF216

    STRING Interaction Network Preview (showing 5 interactants - click image to see 16)

    5/21 Interacting proteins for RNF216 (Q9NWF91, 2, 3 ENSP000003745524) via UniProtKB, MINT, STRING, and/or I2D (see all 21)

    InteractantInteraction Details
    GeneCardExternal ID(s)
    RNF11Q9Y3C52, 3MINT-60865 I2D: score=2 
    TLR9Q9NR963, ENSP000003538744I2D: score=3 STRING: ENSP00000353874
    KIR2DL4Q997063, ENSP000003396344I2D: score=2 STRING: ENSP00000339634
    TIRAPP587533, ENSP000003764454I2D: score=2 STRING: ENSP00000376445
    UBE2L6O149333, ENSP000002871564I2D: score=2 STRING: ENSP00000287156
    About this table

    Gene Ontology (GO): 5/6 biological process terms (GO ID links to tree view) (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006915apoptotic process IEA--
    GO:0019048modulation by virus of host morphology or physiology IEA--
    GO:0032648regulation of interferon-beta production IDA19893624
    GO:0043161proteasomal ubiquitin-dependent protein catabolic process IDA19893624
    GO:0050691regulation of defense response to virus by host IDA19893624

    RNF216 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for RNF216 (RN216)

    Search CenterWatch for drugs/clinical trials and news about RNF216 / RN216

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Sirion Biotech, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for RNF216 gene (3 alternative transcripts): 
    NM_207111.3  NM_207116.2  NM_019011.4  

    Unigene Clusters for RNF216:

    Ring finger protein 216
    Hs.487458  [show with all ESTs], Hs.689456  [show with all ESTs]
    Unigene Representative Sequences: NM_207111, AK128551
    9 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000425013(uc010ksz.2 uc010kta.2 uc003sox.2 uc003soy.2 uc011jwj.2)
    ENST00000389902 ENST00000389900 ENST00000469375 ENST00000484458(uc003spa.1)
    ENST00000479541 ENST00000411812 ENST00000416985 ENST00000476345(uc003spb.3)

    miRNA
    Products:
         
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat RNF216
    8/54 QIAGEN miScript miRNA Assays for microRNAs that regulate RNF216 (see all 54):
    hsa-miR-520e hsa-miR-302d hsa-miR-300 hsa-miR-4328 hsa-miR-199a-3p hsa-miR-301a hsa-miR-519a hsa-miR-766
    SwitchGear 3'UTR luciferase reporter plasmidRNF216 3' UTR sequence
    Inhib. RNA
    Products:
         
    Browse for Gene Knock-down Tools from EMD Millipore
    OriGene RNAi products in human, mouse, rat for RNF216
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat RNF216
    Clone
    Products:
         
    OriGene clones in human, mouse for RNF216 (see all 12)
    OriGene ORF clones in mouse, rat for RNF216
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): RNF216 (NM_207111)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for RNF216
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RNF216
    Sirion Biotech Customized lentivirus for stable overexpression of RNF216 
                         Customized lentivirus expression plasmids for stable overexpression of RNF216 
    Primer
    Products:
        
    OriGene qSTAR qPCR primer pairs in human, mouse for RNF216
    SABiosciences RT2 qPCR Primer Assay in human, mouse, rat RNF216
      QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat RNF216
      QIAGEN QuantiFast Probe-based Assays in human, mouse, rat RNF216

    Additional mRNA sequence: AK128551.1 

    17 DOTS entries:

    DT.100822416  DT.95119108  DT.101984488  DT.100822412  DT.97839502  DT.101971951  DT.121115404  DT.40129927 
    DT.97811158  DT.100009747  DT.100717603  DT.75102250  DT.91757544  DT.92048653  DT.95226826  DT.121115379 
    DT.40297166 

    24/329 AceView cDNA sequences (see all 329):

    BG254095 AI024605 AA912708 AI018503 CA436370 AA804908 F08909 AA746299 
    BE350347 AA278262 CK429258 BU785403 AW207120 BM817557 AI380539 AI916761 
    AI223829 F11246 AK093275 BQ212689 BU187037 BX097482 AI024759 BF055141 

    GeneLoc Exon Structure

    5/10 Alternative Splicing Database (ASD) splice patterns (SP) for RNF216 (see all 10)    About this scheme

    ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7a · 7b · 7c · 7d ^ 8a · 8b ^ 9 ^ 10 ^ 11a · 11b ^ 12 ^ 13 ^ 14 ^ 15 ^ 16 ^ 17a · 17b ^ 18 ^ 19 ^ 20 ^
    SP1:                                      -                       -           -                                                                                 
    SP2:                                                                                                                    -     -                 -               
    SP3:                                                                                                                                                            
    SP4:        -     -     -                                                                                                                                       
    SP5:                                                                                                                                                            

    ExUns: 21a · 21b
    SP1:            
    SP2:            
    SP3:            
    SP4:            
    SP5:            


    ECgene alternative splicing isoforms for RNF216

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    RNF216 expression in normal human tissues (normalized intensities)      RNF216 embryonic expression: see
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: --
    RNF216 Expression
    About this image


    RNF216 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database 
     5/23 selected tissues (see all 23) fully expand
     
     Testis (Reproductive System)    fully expand to see all 3 entries
             Leydig Cells Testis Interstitium
             testis ; cells in seminiferus ducts   
             sperm   
     
     Uterus (Reproductive System)    fully expand to see all 3 entries
             uterus, post-menopause ; glandular cells   
     
     Lung (Respiratory System)    fully expand to see all 3 entries
             lung ; pneumocytes   
     
     Blood (Hematopoietic System)    fully expand to see all 2 entries
             Hematopoietic Stem Cells Hematopoietic Bone Marrow
             lung ; macrophages   
     
     Thyroid (Endocrine System)    fully expand to see all 2 entries
             thyroid gland ; glandular cells   

    See RNF216 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for RNF216

    SOURCE GeneReport for Unigene clusters: Hs.487458 Hs.689456

    UniProtKB/Swiss-Prot: RN216_HUMAN, Q9NWF9
    Tissue specificity: Ubiquitous, with the highest levels of expression in testis and peripheral blood leukocytes

        SABiosciences Expression via Pathway-Focused PCR Array including RNF216: 
              Ubiquitin Ligases in human mouse rat

    Primer
    Products:
    OriGene qSTAR qPCR primer pairs in human, mouse for RNF216
    SABiosciences RT2 qPCR Primer Assay in human, mouse, rat RNF216
    QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat RNF216
    QIAGEN QuantiFast Probe-based Assays in human, mouse, rat RNF216
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RNF216

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of chordates.

    Orthologs for RNF216 gene from 5/12 species (see all 12)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Rnf2161 , 5 ring finger protein 2161, 5 87.29(n)1
    87.8(a)1
      5 (81.86 cM)5
    1080861  NM_207110.11  NP_996993.11 
     1429908935 
    chicken
    (Gallus gallus)
    Aves RNF2161 ring finger protein 216 68.56(n)
    64.82(a)
      416486  XM_414793.3  XP_414793.3 
    lizard
    (Anolis carolinensis)
    Reptilia RNF2166
    Uncharacterized protein
    68(a)
    1 ↔ 1
    GL343725.1(33941-97010)
    African clawed frog
    (Xenopus laevis)
    Amphibia Xl.37422 Xenopus laevis transcribed sequence with weak similarity more 75.19(n)    BJ616422.1 
    zebrafish
    (Danio rerio)
    Actinopterygii Dr.68752 Transcribed sequence with weak similarity to protein more 75.57(n)    57061962 


    ENSEMBL Gene Tree for RNF216 (if available)
    TreeFam Gene Tree for RNF216 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for RNF216 gene
    5 SIMAP similar genes for RNF216 using alignment to 4 protein entries:     RN216_HUMAN (see all proteins):
    RNF216P1    ZNF738    NSRP1    ZNF83    RARS2

    RNF216 for paralogs           About GeneDecksing


    1 Pseudogenes.org Pseudogene for RNF216
    PGOHUM00000260295


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/4140 SNPs in RNF216 are shown (see all 4140)    About this table     
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 7 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1384681161,2
    --5630306(+) GTAGCA/GGGGCC 2 -- ds50010--------
    rs1440290021,2
    --5630368(+) TCTCAA/GCCCAG 2 -- ds50010--------
    rs1147987211,2
    C,F--5630393(+) TGGAAA/C/TCTTGT 2 -- ds50011WA 118
    rs733362241,2
    C,F--5630405(+) CCCTCC/TTCAGC 2 -- ds50013Minor allele frequency- T:0.06WA CSA 122
    rs8524431,2
    C,F,H--5630473(-) TTCTTT/CTGCAG 2 -- ds500112Minor allele frequency- C:0.02NS EA NA 1616
    rs1179800911,2
    F--5630502(+) GCAGGG/AGATTC 2 -- ds50011Minor allele frequency- A:0.01EA 120
    rs733362271,2
    C,F--5630565(+) CCTAGC/TGGGAT 2 -- ds50013Minor allele frequency- T:0.06WA CSA 122
    rs1908391151,2
    C--5630589(+) CACCTC/GATCTT 2 -- ds50010--------
    rs1468979381,2
    --5630592(+) CTGATC/TTTCCT 2 -- ds50010--------
    rs1830734781,2
    --5630597(+) CTTCCA/TCCCTG 2 -- ds50010--------

    HapMap Linkage Disequilibrium report for RNF216 (5659672 - 5821370 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 10/15 variations for RNF216 (see all 15):    About this table     
    Variant IDTypeSubtypePubMed ID
    esv2665342CNV Deletion23128226
    esv2733915CNV Deletion23290073
    nsv436904CNV Insertion17901297
    nsv830890CNV Loss17160897
    dgv7143n71CNV Loss21882294
    nsv887418CNV Loss21882294
    dgv402n21CNV Loss19592680
    dgv7142n71CNV Loss21882294
    nsv887420CNV Gain21882294
    nsv471315CNV Gain18288195

    SABiosciences Cancer Mutation PCR Assays
    Search QIAGEN SeqTarget long-range PCR primers for resequencing RNF216
    DNA2.0 Custom Variant and Variant Library Synthesis for RNF216

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section
    OMIM gene information: 609948    OMIM disorders: --

    2 diseases for RNF216:    About MalaCards
    obesity    gastric cancer


    RNF216 for disorders           About GeneDecksing

    Genetic Association Database (GAD): RNF216
    Human Genome Epidemiology (HuGE) Navigator: RNF216 (3 documents)

    Export disorders for RNF216 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for RNF216 gene, integrated from 9 sources (see all 39):
    (articles sorted by number of sources associating them with RNF216)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Ring finger protein ZIN interacts with human immunodeficiency virus type 1 Vif. (PubMed id 15367624)1, 2, 9 Feng F.... Li P. (2004)
    2. Variation at the NFATC2 locus increases the risk of t hiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipr il and rosiglitazone Medication (DREAM) study. (PubMed id 20628086)1, 4 Bailey S.D....Anand S. (2010)
    3. The E3 ubiquitin ligase Triad3A negatively regulates the RIG-I/MAVS signaling pathway by targeting TRAF3 for degradation. (PubMed id 19893624)1, 2 Nakhaei P....Hiscott J. (2009)
    4. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
    5. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    6. Triad3A, an E3 ubiquitin-protein ligase regulating Toll-like receptors. (PubMed id 15107846)1, 2 Chuang T.-H. and Ulevitch R.J. (2004)
    7. The DNA sequence of human chromosome 7. (PubMed id 12853948)1, 2 Hillier L.W.... Wilson R.K. (2003)
    8. A novel zinc finger protein interacts with receptor-interacting protein (RIP) and inhibits tumor necrosis factor (TNF)- and IL1- induced NF-kappa B activation. (PubMed id 11854271)1, 2 Chen D.... Shu H.-B. (2002)
    9. Ataxia, dementia, and hypogonadotropism caused by diso rdered ubiquitination. (PubMed id 23656588)1 Margolin D.H....Seminara S.B. (2013)
    10. Charting the landscape of tandem BRCT domain-mediated protein interactions. (PubMed id 22990118)1 Woods N.T....Monteiro A.N. (2012)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 54476 HGNC: 21698 AceView: RBAK Ensembl:ENSG00000011275 euGenes: HUgn54476
    ECgene: RNF216 H-InvDB: RNF216

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for RNF216 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for RNF216 gene:
    Search GeneIP for patents involving RNF216

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0 and Sirion Biotech, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Sirion Biotech, Cell lines from GenScript, and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
    About This Section

     
     EMD Millipore Custom Antibody & Bulk Services
     EMD Millipore Preclinical / Clinical Development Services
     EMD Millipore Immunoassay Services
     EMD Millipore Target Screening & Profiling Services

      
     Browse Antibodies   Browse Cell Culture Products  
     Browse ELISAs   Browse Flow Cytometry Kits  
     Browse Primer Pairs   Browse Kinase Activity Assays/Reagents  
     Browse ELISpot Kits/Development Modules   Browse TFB/Immunoprecipitation Assays  
     Browse Apoptosis Detection Kits/Reagents   Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents  
     Browse DNA Damage/Repair Kits/Reagents   Browse Multiplex/Array Assay Kits/Reagents  
     Browse Cell Selection/Detection Kits/Reagents   Browse Secondary Antibodies/Controls/Staining Reagents  
     Browse Protease Activity Assays and Reagents   Browse Recombinant/Natural Proteins  
     Browse Stem Cell Products   Browse Tocris Biochemicals & Compounds  
     Browse cDNA Clones   Browse Proteome Profiler Antibody Arrays  
     OriGene Antibodies for RNF216   OriGene RNAi products in human, mouse, rat for RNF216  
     Browse OriGene qPCR primer pairs and template standards   OriGene Protein Over-expression Lysate for RNF216  
     OriGene Custom Mass Spec   OriGene clones in human, mouse for RNF216  
     OriGene qSTAR qPCR primer pairs in human, mouse for RNF216   Browse OriGene full length recombinant human proteins expressed in human HEK293 cells  
     OriGene ORF clones in mouse, rat for RNF216   OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling  
     OriGene Custom Antibody Services for RNF216   OriGene Custom Protein Services for RNF216  

     
     
     QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat RNF216
     QIAGEN SeqTarget long-range PCR primers for resequencing RNF216
     QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat RNF216
     QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat RNF216
     QIAGEN QuantiFast Probe-based Assays in human, mouse, rat RNF216
     QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat RNF216
     GenScript Custom Purified and Recombinant Proteins Services for RNF216 GenScript cDNA clones with any tag delivered in your preferred vector for RNF216
     GenScript Custom Assay Services for RNF216 GenScript Custom Superior Antibodies Services for RNF216
     GenScript Custom overexpressing Cell Line Services for RNF216 CloneReady with Over 120,000 Genes
     Gene Synthesis: Any Gene in Any Vector Vector-based siRNA and miRNA, Ready for Transfection
     Gene Mutant Library, Variants up to 10^11 Plasmid Preparation
     Custom Peptide Services
     Search for Antibodies & Assays

     Regulatory tfbs in RNF216 promoter
     Search Chromatin IP Primers for RNF216
     RT2 qPCR Primer Assay in human, mouse, rat RNF216
     Search GNC Networks for RNF216
     SABiosciences PCR Arrays including human, mouse, rat RNF216
     Search Tocris compounds for RNF216 (RN216)
     Browse Sino Biological Proteins and Antibodies
     Browse Sino Biological Cell Lysates
     Browse Sino Biological cDNA Clones
     4000+ Proteins
     Search Sino Biological for antibodies, proteins & pathways
     Protein Production Services
     Transfection Reagents
     Protein A/G/L resins
     Isotyping reagents
     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies
     Novus Tissue Slides
     RNF216 antibodies
     RNF216 lysates
     Antibodies for RNF216
     See all of Abcam's Antibodies, Kits and Proteins for RNF216
     Custom Antibody / Protein Production Service
     Bulk Purchasing
     Advantages of Rabbit Monoclonal antibodies
     Abcam protocols and scientific support
     Browse ProSpec Recombinant Proteins




     Proteins for RNF216
     Antibodies for RNF216
     ELISAs for RNF216
     CLIAs for RNF216
     Search LifeMap BioReagents cell lines for RNF216
     Gene Synthesis
     Protein Engineering
     Variant Library Synthesis
     Codon Optimization
     Protein Production and Purification
     Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RNF216
     SwitchGear 3'UTR luciferase reporter plasmids for RNF216
     SwitchGear Promoter luciferase reporter plasmids for RNF216
     ThermoFisher Antibodies for RNF216
     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RNF216
     inGenious Targeting Laboratory - Custom generated mouse model solutions for RNF216
     inGenious Targeting Laboratory - Custom generated inducible mouse model solutions for RNF216
    Customized:
     lentivirus for stable overexpression of RNF216
     lentivirus expression plasmids for stable overexpression of RNF216
     adenovirus for overexpression of RNF216
     LSBio Antibodies in human, mouse, rat for RNF216
    Customized transgenic rodents for:
     Humanization
     Biomarker expression
     Off-target effect monitoring
     Translational medicine
     Tissue-specific gene expresssion
     Time-controlled gene expresssion
           
    GeneCards Homepage - Last full update: 23 Oct 2013 - Incrementals: 3 Nov 2013 , 7 Nov 2013 , 23 Jan 2014

    View Random Gene

    Category
    (GIFtS: 73)
    transforming growth factor, beta 1
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 6 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 


    Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

    Hot genes      Disease genes      RNF216 gene at Home site.
    hostname: 356977-web1.xennexinc.com index build: 106 solr: 1.4