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Aliases for RNF216 Gene

Aliases for RNF216 Gene

  • Ring Finger Protein 216 2 3
  • TRIAD3 3 4 6
  • ZIN 3 4 6
  • Ubiquitin-Conjugating Enzyme 7-Interacting Protein 1 3 4
  • Zinc Finger Protein Inhibiting NF-Kappa-B 3 4
  • Triad Domain-Containing Protein 3 3 4
  • UBCE7IP1 3 4
  • CAHH 3 6
  • E3 Ubiquitin-Protein Ligase RNF216 3
  • RING Finger Protein 216 4
  • EC 6.3.2.- 4
  • U7I1 3

External Ids for RNF216 Gene

Previous GeneCards Identifiers for RNF216 Gene

  • GC07M005627

Summaries for RNF216 Gene

Entrez Gene Summary for RNF216 Gene

  • This gene encodes a cytoplasmic protein which specifically colocalizes and interacts with the serine/threonine protein kinase, receptor-interacting protein (RIP). Zinc finger domains of the encoded protein are required for its interaction with RIP and for inhibition of TNF- and IL1-induced NF-kappa B activation pathways. The encoded protein may also function as an E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes and transfers it to substrates. Several alternatively spliced transcript variants have been described for this locus but the full-length natures of only some are known. [provided by RefSeq, Jul 2008]

GeneCards Summary for RNF216 Gene

RNF216 (Ring Finger Protein 216) is a Protein Coding gene. Diseases associated with RNF216 include cerebellar ataxia and hypogonadotropic hypogonadism and hypogonadotropism. Among its related pathways are TNF-alpha/NF-kB Signaling Pathway and TRAF Pathway. GO annotations related to this gene include ligase activity.

UniProtKB/Swiss-Prot for RNF216 Gene

  • Isoform 1 acts as an E3 ubiquitin ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Promotes degradation of TRAF3, TLR4 and TLR9. Contributes to the regulation of antiviral responses. Down-regulates activation of NF-kappa-B, IRF3 activation and IFNB production. Isoform 3/ZIN inhibits TNF and IL-1 mediated activation of NF-kappa-B. Promotes TNF and RIP mediated apoptosis.

Gene Wiki entry for RNF216 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for RNF216 Gene

Genomics for RNF216 Gene

Regulatory Elements for RNF216 Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for RNF216 Gene

Start:
5,620,041 bp from pter
End:
5,781,739 bp from pter
Size:
161,699 bases
Orientation:
Minus strand

Genomic View for RNF216 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for RNF216 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RNF216 Gene

Proteins for RNF216 Gene

  • Protein details for RNF216 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9NWF9-RN216_HUMAN
    Recommended name:
    E3 ubiquitin-protein ligase RNF216
    Protein Accession:
    Q9NWF9
    Secondary Accessions:
    • Q6Y691
    • Q75ML7
    • Q7Z2H7
    • Q7Z7C1
    • Q8NHW7
    • Q9NYT1

    Protein attributes for RNF216 Gene

    Size:
    866 amino acids
    Molecular mass:
    99406 Da
    Quaternary structure:
    • Interacts with UBE2L3 and to some extent with UBE2L6. Interacts with TRAF3, TLR3, TLR4, TLR5 and TLR9. Isoform 3/ZIN binds RIPK1 and HIV VIF.
    SequenceCaution:
    • Sequence=BAA91422.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};

    Alternative splice isoforms for RNF216 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for RNF216 Gene

Proteomics data for RNF216 Gene at MOPED

Post-translational modifications for RNF216 Gene

  • Auto-ubiquitinated
  • Ubiquitination at Lys408, Lys411, Lys419, Lys448, Lys482, Lys611, Lys641, Lys676, Lys715, Lys723, and Lys830
  • Modification sites at PhosphoSitePlus

Other Protein References for RNF216 Gene

No data available for DME Specific Peptides for RNF216 Gene

Domains for RNF216 Gene

Gene Families for RNF216 Gene

HGNC:
  • RNF :RING-type (C3HC4) zinc fingers

Protein Domains for RNF216 Gene

Suggested Antigen Peptide Sequences for RNF216 Gene

Graphical View of Domain Structure for InterPro Entry

Q9NWF9

UniProtKB/Swiss-Prot:

RN216_HUMAN :
  • Q9NWF9
Domain:
  • The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
Similarity:
  • Contains 1 IBR-type zinc finger.
  • Contains 2 RING-type zinc fingers.
genes like me logo Genes that share domains with RNF216: view

Function for RNF216 Gene

Molecular function for RNF216 Gene

UniProtKB/Swiss-Prot Function: Isoform 1 acts as an E3 ubiquitin ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Promotes degradation of TRAF3, TLR4 and TLR9. Contributes to the regulation of antiviral responses. Down-regulates activation of NF-kappa-B, IRF3 activation and IFNB production. Isoform 3/ZIN inhibits TNF and IL-1 mediated activation of NF-kappa-B. Promotes TNF and RIP mediated apoptosis.

Enzyme Numbers (IUBMB) for RNF216 Gene

Gene Ontology (GO) - Molecular Function for RNF216 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005515 protein binding IPI 12600646
GO:0008270 zinc ion binding IEA --
GO:0016874 ligase activity IEA --
genes like me logo Genes that share ontologies with RNF216: view

Phenotypes for RNF216 Gene

GenomeRNAi human phenotypes for RNF216:
genes like me logo Genes that share phenotypes with RNF216: view

Animal Model Products

CRISPR Products

miRNA for RNF216 Gene

miRTarBase miRNAs that target RNF216

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for RNF216

In Situ Assay Products

Flow Cytometry Products

No data available for Animal Models , Transcription Factor Targeting and HOMER Transcription for RNF216 Gene

Localization for RNF216 Gene

Subcellular locations from UniProtKB/Swiss-Prot for RNF216 Gene

Cytoplasm.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for RNF216 Gene COMPARTMENTS Subcellular localization image for RNF216 gene
Compartment Confidence
cytosol 4
nucleus 4
mitochondrion 1

Gene Ontology (GO) - Cellular Components for RNF216 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IDA --
GO:0005654 nucleoplasm IDA --
GO:0005737 cytoplasm IDA --
genes like me logo Genes that share ontologies with RNF216: view

Pathways for RNF216 Gene

genes like me logo Genes that share pathways with RNF216: view

Pathways by source for RNF216 Gene

1 Qiagen pathway for RNF216 Gene
2 BioSystems pathways for RNF216 Gene

PCR Array Products

  • Pathway & Disease-focused RT² Profiler PCR Arrays

UniProtKB/Swiss-Prot Q9NWF9-RN216_HUMAN

  • Pathway: Protein modification; protein ubiquitination

Gene Ontology (GO) - Biological Process for RNF216 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006915 apoptotic process IEA --
GO:0016032 viral process IEA --
GO:0032648 regulation of interferon-beta production IDA 19893624
GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process IDA 19893624
GO:0050691 regulation of defense response to virus by host IDA 19893624
genes like me logo Genes that share ontologies with RNF216: view

Transcripts for RNF216 Gene

Unigene Clusters for RNF216 Gene

Ring finger protein 216:
Representative Sequences:

CRISPR Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for RNF216

Primer Products

  • OriGene qSTAR qPCR primer pairs in human,mouse,rat for RNF216

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for RNF216 Gene

ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7a · 7b · 7c · 7d ^ 8a · 8b ^ 9 ^ 10 ^ 11a · 11b ^ 12 ^ 13 ^ 14 ^ 15 ^ 16 ^ 17a · 17b ^ 18 ^ 19 ^ 20 ^
SP1: - - -
SP2: - - -
SP3:
SP4: - - -
SP5:
SP6: -
SP7: - -
SP8: -
SP9:
SP10:

ExUns: 21a · 21b
SP1:
SP2:
SP3:
SP4:
SP5:
SP6:
SP7:
SP8:
SP9:
SP10:

Relevant External Links for RNF216 Gene

GeneLoc Exon Structure for
RNF216
ECgene alternative splicing isoforms for
RNF216

Expression for RNF216 Gene

mRNA expression in normal human tissues for RNF216 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for RNF216 Gene

SOURCE GeneReport for Unigene cluster for RNF216 Gene Hs.487458

mRNA Expression by UniProt/SwissProt for RNF216 Gene

Q9NWF9-RN216_HUMAN
Tissue specificity: Ubiquitous, with the highest levels of expression in testis and peripheral blood leukocytes
genes like me logo Genes that share expressions with RNF216: view

In Situ Assay Products

No data available for mRNA differential expression in normal tissues for RNF216 Gene

Orthologs for RNF216 Gene

This gene was present in the common ancestor of chordates.

Orthologs for RNF216 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia RNF216 35
  • 99.64 (n)
  • 99.78 (a)
RNF216 36
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia RNF216 35
  • 87.5 (n)
  • 88.66 (a)
RNF216 36
  • 90 (a)
OneToOne
dog
(Canis familiaris)
Mammalia RNF216 35
  • 91.4 (n)
  • 93.82 (a)
RNF216 36
  • 93 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Rnf216 35
  • 87.36 (n)
  • 87.8 (a)
Rnf216 16
Rnf216 36
  • 88 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia -- 36
  • 86 (a)
OneToMany
-- 36
  • 56 (a)
OneToMany
platypus
(Ornithorhynchus anatinus)
Mammalia RNF216 36
  • 75 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Rnf216 35
  • 86.56 (n)
  • 87.47 (a)
chicken
(Gallus gallus)
Aves -- 36
  • 62 (a)
OneToMany
-- 36
  • 86 (a)
OneToMany
RNF216 35
  • 68.53 (n)
  • 65.24 (a)
lizard
(Anolis carolinensis)
Reptilia RNF216 36
  • 68 (a)
OneToOne
African clawed frog
(Xenopus laevis)
Amphibia Xl.3742 35
tropical clawed frog
(Silurana tropicalis)
Amphibia rnf216 35
  • 63.75 (n)
  • 62.56 (a)
Str.14705 35
zebrafish
(Danio rerio)
Actinopterygii Dr.6875 35
rnf216 35
  • 61.07 (n)
  • 57.36 (a)
rnf216 36
  • 47 (a)
OneToOne
Species with no ortholog for RNF216:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for RNF216 Gene

ENSEMBL:
Gene Tree for RNF216 (if available)
TreeFam:
Gene Tree for RNF216 (if available)

Paralogs for RNF216 Gene

Selected SIMAP similar genes for RNF216 Gene using alignment to 4 proteins:

Pseudogenes.org Pseudogenes for RNF216 Gene

genes like me logo Genes that share paralogs with RNF216: view

No data available for Paralogs for RNF216 Gene

Variants for RNF216 Gene

Sequence variations from dbSNP and Humsavar for RNF216 Gene

SNP ID Clin Chr 07 pos Sequence Context AA Info Type MAF
rs9401 -- 5,620,154(+) ACCAG(A/T)AGGTA utr-variant-3-prime
rs710939 -- 5,651,059(+) ATATA(C/T)GTATA intron-variant
rs710940 -- 5,634,204(+) GACCA(A/C/G/T)GGGGG intron-variant
rs710941 -- 5,633,798(+) GGCCT(C/T)TCCAG intron-variant
rs752909 -- 5,726,328(+) TCACA(C/T)GACAA intron-variant

Structural Variations from Database of Genomic Variants (DGV) for RNF216 Gene

Variant ID Type Subtype PubMed ID
nsv436904 CNV Insertion 17901297
dgv7142n71 CNV Loss 21882294
nsv830890 CNV Loss 17160897
dgv7143n71 CNV Loss 21882294
nsv887417 CNV Gain 21882294
nsv887418 CNV Loss 21882294
nsv887419 CNV Gain 21882294
nsv830891 CNV Gain 17160897
esv2665342 CNV Deletion 23128226
dgv798n27 CNV Gain 19166990
nsv464296 CNV Gain 19166990
nsv471315 CNV Gain 18288195
esv2733915 CNV Deletion 23290073
nsv887420 CNV Gain 21882294
dgv402n21 CNV Loss 19592680

Relevant External Links for RNF216 Gene

HapMap Linkage Disequilibrium report
RNF216
Human Gene Mutation Database (HGMD)
RNF216

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for RNF216 Gene

Disorders for RNF216 Gene

MalaCards: The human disease database

MalaCards: The human disease database. (2) Diseases for RNF216 Gene including...

Search for RNF216 Gene in MalaCards »

(1) OMIM Diseases for RNF216 Gene (609948)

UniProtKB/Swiss-Prot

RN216_HUMAN
  • Gordon Holmes syndrome (GDHS) [MIM:212840]: A disease characterized by cerebellar symptoms and signs of sex steroid deficiency. Clinical features include cerebellar and brain stem atrophy, cerebellar ataxia, hypothalamic LHRH deficiency, hypogonadotrophic hypogonadism, lack of secondary sexual characteristics, and infertility. {ECO:0000269 PubMed:23656588}. Note=The disease is caused by mutations affecting the gene represented in this entry.

Relevant External Links for RNF216

Genetic Association Database (GAD)
RNF216
Human Genome Epidemiology (HuGE) Navigator
RNF216
genes like me logo Genes that share disorders with RNF216: view

Publications for RNF216 Gene

  1. Ring finger protein ZIN interacts with human immunodeficiency virus type 1 Vif. (PMID: 15367624) Feng F. … Li P. (J. Virol. 2004) 3 4 23
  2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard D.S. … Malek J. (Genome Res. 2004) 3 4
  3. The DNA sequence of human chromosome 7. (PMID: 12853948) Hillier L.W. … Wilson R.K. (Nature 2003) 3 4
  4. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T. … Sugano S. (Nat. Genet. 2004) 3 4
  5. Triad3A, an E3 ubiquitin-protein ligase regulating Toll-like receptors. (PMID: 15107846) Chuang T.-H. … Ulevitch R.J. (Nat. Immunol. 2004) 3 4

Products for RNF216 Gene

  • antibodies-online peptides for RNF216
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  • antibodies-online kits for RNF216

Sources for RNF216 Gene

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