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RNF214 Gene

protein-coding   GIFtS: 47
GCID: GC11P117104

Ring Finger Protein 214

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disease database 

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Ring Finger Protein 2141 2

External Ids:    HGNC: 253351   Entrez Gene: 2571602   Ensembl: ENSG000001672577   UniProtKB: Q8ND243   

Export aliases for RNF214 gene to outside databases

Previous GC identifers: GC11P116609 GC11P113036


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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GeneCards Summary for RNF214 Gene:
RNF214 (ring finger protein 214) is a protein-coding gene. An important paralog of this gene is TTC3.


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000011.9  NT_033899.9  NC_018922.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the RNF214 gene promoter:
         AML1a   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidRNF214 promoter sequence
   Search Chromatin IP Primers for RNF214

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat RNF214


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 11q23.3   Ensembl cytogenetic band:  11q23.3   HGNC cytogenetic band: 11q23.3

RNF214 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
RNF214 gene location

GeneLoc information about chromosome 11         GeneLoc Exon Structure

GeneLoc location for GC11P117104:  view genomic region     (about GC identifiers)

Start:
117,103,341 bp from pter      End:
117,157,161 bp from pter
Size:
53,821 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., eBioscience, and/or antibodies-online,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., eBioscience, and/or antibodies-online, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, Cloud-Clone Corp, antibodies-online, and/or others.)
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UniProtKB/Swiss-Prot: RN214_HUMAN, Q8ND24 (See protein sequence)
Recommended Name: RING finger protein 214  
Size: 703 amino acids; 77667 Da
Secondary accessions: B2RUW0

Explore the universe of human proteins at neXtProt for RNF214: NX_Q8ND24

Explore proteomics data for RNF214 at MOPED

Post-translational modifications: 

  • Ubiquitination2 at Lys259
  • Modification sites at PhosphoSitePlus

  • See RNF214 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (3 alternative transcripts): 
    NP_001070707.1  NP_001265178.1  NP_997226.2  

    ENSEMBL proteins: 
     ENSP00000434186   ENSP00000435361   ENSP00000431643   ENSP00000432903   ENSP00000300650  

    RNF214 Human Recombinant Protein Products:

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    Cloud-Clone Corp. Proteins for RNF214

     
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    antibodies-online proteins for RNF214 (2 products) 

     
    antibodies-online peptides for RNF214

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    Cloud-Clone Corp. ELISAs for RNF214
    Cloud-Clone Corp. CLIAs for RNF214
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    antibodies-online kits for RNF214 (4 products) 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    HGNC Gene Families:
    RNF: RING-type (C3HC4) zinc fingers

    2 InterPro protein domains:
     IPR001841 Znf_RING
     IPR013083 Znf_RING/FYVE/PHD

    Graphical View of Domain Structure for InterPro Entry Q8ND24

    ProtoNet protein and cluster: Q8ND24

    1 Blocks protein domain: IPB001841 Zn-finger

    UniProtKB/Swiss-Prot: RN214_HUMAN, Q8ND24
    Similarity: Contains 1 RING-type zinc finger


    Find genes that share domains with RNF214           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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         Gene Ontology (GO): 2 molecular function terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515protein binding ----
    GO:0008270zinc ion binding IEA--
         
    Find genes that share ontologies with RNF214           About GenesLikeMe


    Phenotypes:
         2 GenomeRNAi human phenotypes for RNF214:
     Decreased viability with pacli  Increased TP53 protein express 

    Animal Models:
       genOway: Develop your customized and physiologically relevant rodent model for RNF214

    miRNA
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    miRTarBase miRNAs that target RNF214:
    hsa-mir-335-5p (MIRT016867)

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    Selected qRT-PCR Assays for microRNAs that regulate RNF214 (see all 16):
    hsa-miR-323-3p hsa-miR-362-3p hsa-miR-374b hsa-miR-29a hsa-miR-29c hsa-miR-374a hsa-miR-410 hsa-miR-1284
    SwitchGear 3'UTR luciferase reporter plasmidRNF214 3' UTR sequence
    Inhib. RNA
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    OriGene RNAi products in human, mouse, rat for RNF214
    Predesigned siRNA for gene silencing in human, mouse, rat RNF214

    Gene Editing
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    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    cytosol2
    nucleus2
    golgi apparatus1

    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for RNF214
    Interactions:

        Search GeneGlobe Interaction Network for RNF214

    STRING Interaction Network Preview (showing 1 interactants - click image to see more details)

    2 Interacting proteins for RNF214 (Q8ND243 ENSP000003006504) via UniProtKB, MINT, STRING, and/or I2D
    InteractantInteraction Details
    GeneCardExternal ID(s)
    DDA1Q9BW613I2D: score=1 
    UBCENSP000003448184STRING: ENSP00000344818
    About this table

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for RNF214 (RN214)



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for RNF214 gene (3 alternative transcripts): 
    NM_001077239.1  NM_001278249.1  NM_207343.3  

    Unigene Cluster for RNF214:

    Ring finger protein 214
    Hs.591934  [show with all ESTs]
    Unigene Representative Sequence: NM_001077239
    8 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000534428 ENST00000529869 ENST00000531287 ENST00000531452(uc001pqt.3 uc001pqu.3 uc010rxf.2)
    ENST00000530849 ENST00000524917 ENST00000534709 ENST00000300650
    miRNA
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    Selected qRT-PCR Assays for microRNAs that regulate RNF214 (see all 16):
    hsa-miR-323-3p hsa-miR-362-3p hsa-miR-374b hsa-miR-29a hsa-miR-29c hsa-miR-374a hsa-miR-410 hsa-miR-1284
    SwitchGear 3'UTR luciferase reporter plasmidRNF214 3' UTR sequence
    Inhib. RNA
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    Predesigned siRNA for gene silencing in human, mouse, rat RNF214
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    GenScript: all cDNA clones in your preferred vector (see all 2): RNF214 (NM_001077239)
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    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RNF214
    Primer
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    OriGene qSTAR qPCR primer pairs in human, mouse for RNF214
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat RNF214
      QuantiTect SYBR Green Assays in human, mouse, rat RNF214
      QuantiFast Probe-based Assays in human, mouse, rat RNF214

    Additional mRNA sequence: 

    AK298923.1 AK300160.1 AL834448.1 BC031347.1 BC064581.1 BC146891.1 BC146901.1 

    7 DOTS entries:

    DT.95289355  DT.442123  DT.120701571  DT.100774642  DT.97779002  DT.97834575  DT.91643276 

    Selected AceView cDNA sequences (see all 94):

    AI472598 BU618691 CR624116 BC031347 BM055358 H00530 BE328485 BQ684591 
    AL834448 NM_207343 AV652165 CD742563 CK904824 AA227597 BF437757 CB152535 
    AI951346 BX390413 BX330939 BQ006398 BM853594 BM996401 AA629249 AI151014 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for RNF214 (see all 8)    About this scheme

    ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5a · 5b · 5c ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11a · 11b ^ 12 ^ 13a · 13b · 13c · 13d ^ 14 ^ 15 ^ 16 ^ 17a · 17b
    SP1:        -                                                                                                                                       
    SP2:                                                                                                                                                
    SP3:                                                                                                                                                
    SP4:                                                                                                                                                
    SP5:                                                                                                                          -                     


    ECgene alternative splicing isoforms for RNF214

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    RNF214 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: TATGCAGATT
    RNF214 Expression
    About this image

    RNF214 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    RNF214 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.591934
        Custom PCR Arrays for RNF214
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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RNF214

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of chordates.

    Orthologs for RNF214 gene from Selected species (see all 10)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Rnf2141 , 5 ring finger protein 2141, 5 89.82(n)1
    92.81(a)1
      9 (25.26 cM)5
    2353151  NM_178709.41  NP_848824.11 
     458634255 
    chicken
    (Gallus gallus)
    Aves RNF2141 ring finger protein 214 76.2(n)
    84.22(a)
      419767  XM_004948090.1  XP_004948147.1 
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia rnf2141 ring finger protein 214 56.19(n)
    46.71(a)
      100379742  XM_004916074.1  XP_004916131.1 
    zebrafish
    (Danio rerio)
    Actinopterygii si:dkey-111e8.11 si:dkey-111e8.1 46.78(n)
    31.02(a)
      323719  XM_001921490.4  XP_001921525.3 


    ENSEMBL Gene Tree for RNF214 (if available)
    TreeFam Gene Tree for RNF214 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for RNF214 gene
    TTC32  DZIP32  

    Find genes that share paralogs with RNF214           About GenesLikeMe



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for RNF214 (see all 1155)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 11 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1883375011,2
    --117104851(+) GTATTC/TTCTCC 2 -- int10--------
    rs1410346791,2
    --117104857(+) TCTCCC/TGGAGC 2 -- int10--------
    rs1175479811,2
    C,F--117104900(+) CTGTTG/ATGTGG 2 -- int11Minor allele frequency- A:0.02NA 120
    rs1441592401,2
    C,F--117104952(+) AAACTT/CGTAGC 2 -- int11Minor allele frequency- C:0.00EU 593
    rs1473530111,2
    C--117105004(+) CTGAGG/TTTGCT 4 V F mis10--------
    rs2017196001,2
    C--117105015(+) GGTGTC/TGTGGC 4 V syn10--------
    rs1915458901,2
    C--117105029(+) TGCCCC/GCAGTC 4 P R mis10--------
    rs1395032381,2
    C,F--117105034(+) CCAGTC/TCTCCG 4 P S mis11Minor allele frequency- T:0.00EU 1317
    rs1841630251,2
    --117105059(+) ATGTGC/TTTCCA 4 A V mis10--------
    rs1451197491,2
    C--117105229(+) GATCTA/GTCTTT 2 -- int10--------

    HapMap Linkage Disequilibrium report for RNF214 (117103341 - 117157161 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 3 variations for RNF214:    About this table    
    Variant IDTypeSubtypePubMed ID
    nsv513334CNV Insertion21212237
    nsv509443CNV Insertion20534489
    nsv516483CNV Gain19592680

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing RNF214
    DNA2.0 Custom Variant and Variant Library Synthesis for RNF214

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    Find genes that share disorders with RNF214           About GenesLikeMe

    Genetic Association Database (GAD): RNF214

    Export disorders for RNF214 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for RNF214 gene, integrated from 10 sources (see all 16):
    (articles sorted by number of sources associating them with RNF214)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Genetic determinants of cardiovascular events among women with migraine: a genome-wide association study. (PubMed id 21779381)1, 4 SchA1rks M....Kurth T. (PLoS ONE 2011)
    2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    3. A census of human soluble protein complexes. (PubMed id 22939629)1 Havugimana P.C....Emili A. (Cell 2012)
    4. Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass. (PubMed id 23000965)1 Povlsen L.K....Choudhary C. (Nat. Cell Biol. 2012)
    5. Initial characterization of the human central proteome. (PubMed id 21269460)2 Burkard T.R.... Colinge J. (BMC Syst. Biol. 2011)
    6. Activity-based chemical proteomics accelerates inhibitor development for deubiquitylating enzymes. (PubMed id 22118674)1 Altun M....Kessler B.M. (amp 2011)
    7. A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles. (PubMed id 21890473)1 Wagner S.A....Choudhary C. (amp 2011)
    8. Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. (PubMed id 20068231)2 Olsen J.V....Mann M. (Sci. Signal. 2010)
    9. Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. (PubMed id 19413330)2 Gauci S....Mohammed S. (Anal. Chem. 2009)
    10. Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. (PubMed id 19690332)2 Mayya V.... Han D.K. (Sci. Signal. 2009)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 257160 HGNC: 25335 AceView: DKFZp547C195 Ensembl:ENSG00000167257 euGenes: HUgn257160
    ECgene: RNF214 H-InvDB: RNF214

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for RNF214 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for RNF214 gene:
    Search GeneIP for patents involving RNF214

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    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, eBioscience, antibodies-online, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
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