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Aliases for RNF20 Gene

Aliases for RNF20 Gene

  • Ring Finger Protein 20, E3 Ubiquitin Protein Ligase 2 3
  • BRE1-A 3 4
  • BRE1A 3 4
  • HBRE1 3 4
  • BRE1 E3 Ubiquitin Ligase Homolog (S. Cerevisiae) 2
  • BRE1 E3 Ubiquitin Ligase Homolog 3
  • Homolog Of S. Cerevisiae BRE1 3
  • Ring Finger Protein 20 2
  • RING Finger Protein 20 4
  • EC 6.3.2.- 4
  • EC 6.3.2 63
  • BRE1 3

External Ids for RNF20 Gene

Previous GeneCards Identifiers for RNF20 Gene

  • GC09P095096
  • GC09P096039
  • GC09P097753
  • GC09P099675
  • GC09P101375
  • GC09P103335
  • GC09P104296
  • GC09P073896

Summaries for RNF20 Gene

Entrez Gene Summary for RNF20 Gene

  • The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. The protein encoded by this human gene is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. This protein acts as a putative tumor suppressor and positively regulates the p53 tumor suppressor as well as numerous histone H2A and H2B genes. In contrast, this protein also suppresses the expression of several protooncogenes and growth-related genes, including many genes that are induced by epidermal growth factor. This gene selectively suppresses the expression of some genes by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS). [provided by RefSeq, Feb 2012]

GeneCards Summary for RNF20 Gene

RNF20 (Ring Finger Protein 20, E3 Ubiquitin Protein Ligase) is a Protein Coding gene. Among its related pathways are Chromatin Regulation / Acetylation. GO annotations related to this gene include transcription factor activity, sequence-specific DNA binding and ligase activity. An important paralog of this gene is RNF40.

UniProtKB/Swiss-Prot for RNF20 Gene

  • Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of Lys-120 of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 Lys-4 and Lys-79 methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.

Gene Wiki entry for RNF20 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for RNF20 Gene

Genomics for RNF20 Gene

Regulatory Elements for RNF20 Gene

Genomic Location for RNF20 Gene

Chromosome:
9
Start:
101,533,849 bp from pter
End:
101,563,344 bp from pter
Size:
29,496 bases
Orientation:
Plus strand

Genomic View for RNF20 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for RNF20 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RNF20 Gene

Proteins for RNF20 Gene

  • Protein details for RNF20 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q5VTR2-BRE1A_HUMAN
    Recommended name:
    E3 ubiquitin-protein ligase BRE1A
    Protein Accession:
    Q5VTR2
    Secondary Accessions:
    • A7MCT5
    • Q2TB34
    • Q69YL5
    • Q6P527
    • Q8N3J4
    • Q96JD3
    • Q9H9Y7
    • Q9HA51
    • Q9NUR4
    • Q9NWQ3
    • Q9NX83

    Protein attributes for RNF20 Gene

    Size:
    975 amino acids
    Molecular mass:
    113662 Da
    Quaternary structure:
    • Component of the RNF20/40 complex (also known as BRE1 complex) probably composed of 2 copies of RNF20/BRE1A and 2 copies of RNF40/BRE1B. Interacts with UBE2E1/UBCH6. Interacts with p53/TP53 and WAC. Interacts with PAF1; the interaction mediates the association of the PAF1 and RNF20/40 complexes which is a prerequsite for recruitment of UBE2A/B. Interacts with isoform 1 and isoform 2 of PA2G4.
    SequenceCaution:
    • Sequence=AAH63115.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence.; Evidence={ECO:0000305}; Sequence=BAA91326.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=BAA92057.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=BAB14005.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};

neXtProt entry for RNF20 Gene

Proteomics data for RNF20 Gene at MOPED

Post-translational modifications for RNF20 Gene

  • Ubiquitination at Lys 194, Lys 242, and Lys 821
  • Modification sites at PhosphoSitePlus

Other Protein References for RNF20 Gene

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for RNF20 (RNF20)

Domains & Families for RNF20 Gene

Gene Families for RNF20 Gene

Protein Domains for RNF20 Gene

Suggested Antigen Peptide Sequences for RNF20 Gene

Graphical View of Domain Structure for InterPro Entry

Q5VTR2

UniProtKB/Swiss-Prot:

BRE1A_HUMAN :
  • Belongs to the BRE1 family.
  • Contains 1 RING-type zinc finger.
Family:
  • Belongs to the BRE1 family.
Similarity:
  • Contains 1 RING-type zinc finger.
genes like me logo Genes that share domains with RNF20: view

Function for RNF20 Gene

Molecular function for RNF20 Gene

UniProtKB/Swiss-Prot Function:
Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of Lys-120 of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 Lys-4 and Lys-79 methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.

Enzyme Numbers (IUBMB) for RNF20 Gene

Gene Ontology (GO) - Molecular Function for RNF20 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0002039 p53 binding IDA 16337599
GO:0003682 chromatin binding IEA --
GO:0003713 transcription coactivator activity IDA 16337599
GO:0003730 mRNA 3-UTR binding IDA 19575011
GO:0004842 contributes_to ubiquitin-protein transferase activity IDA 16307923
genes like me logo Genes that share ontologies with RNF20: view
genes like me logo Genes that share phenotypes with RNF20: view

Animal Model Products

miRNA for RNF20 Gene

miRTarBase miRNAs that target RNF20

No data available for Animal Models , Transcription Factor Targets and HOMER Transcription for RNF20 Gene

Localization for RNF20 Gene

Subcellular locations from UniProtKB/Swiss-Prot for RNF20 Gene

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for RNF20 Gene COMPARTMENTS Subcellular localization image for RNF20 gene
Compartment Confidence
nucleus 5

Gene Ontology (GO) - Cellular Components for RNF20 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000151 ubiquitin ligase complex IDA 16307923
GO:0005634 nucleus IDA 16307923
GO:0005654 nucleoplasm IDA --
GO:0005730 nucleolus IDA 19037095
GO:0033503 HULC complex IDA 19410543
genes like me logo Genes that share ontologies with RNF20: view

Pathways & Interactions for RNF20 Gene

genes like me logo Genes that share pathways with RNF20: view

Pathways by source for RNF20 Gene

1 Cell Signaling Technology pathway for RNF20 Gene

PCR Array Products

  • Pathway & Disease-focused RT² Profiler PCR Arrays
    • Epigenetic Chromatin Modification Enzymes in human,mouse,rat
    • Ubiquitin Ligases in human,mouse,rat

UniProtKB/Swiss-Prot Q5VTR2-BRE1A_HUMAN

  • Pathway: Protein modification; protein ubiquitination

SIGNOR curated interactions for RNF20 Gene

Is activated by:

Gene Ontology (GO) - Biological Process for RNF20 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000209 protein polyubiquitination IDA 19037095
GO:0006355 regulation of transcription, DNA-templated IMP 18832071
GO:0006511 ubiquitin-dependent protein catabolic process IMP 19037095
GO:0007346 NOT regulation of mitotic cell cycle IMP 19575011
GO:0010390 histone monoubiquitination IDA 16307923
genes like me logo Genes that share ontologies with RNF20: view

Drugs & Compounds for RNF20 Gene

(3) Drugs for RNF20 Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Adenosine monophosphate Approved Nutra 0
Adenosine triphosphate Approved Nutra 0
Diphosphate Experimental Pharma 0

(1) Additional Compounds for RNF20 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
phosphoric acid
  • Acide phosphorique (FRENCH)
  • Acido fosforico [Italian]
  • Acidum phosphoricum
  • Diphosphate tetrasodium
  • Fosforzuuroplossingen [Dutch]
7664-38-2
genes like me logo Genes that share compounds with RNF20: view

Transcripts for RNF20 Gene

Unigene Clusters for RNF20 Gene

Ring finger protein 20, E3 ubiquitin protein ligase:
Representative Sequences:

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for RNF20

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for RNF20 Gene

ExUns: 1 ^ 2a · 2b ^ 3 ^ 4 ^ 5 ^ 6a · 6b ^ 7a · 7b ^ 8a · 8b · 8c · 8d ^ 9a · 9b · 9c · 9d · 9e ^ 10a · 10b ^ 11a · 11b ^ 12a · 12b ^ 13 ^
SP1:
SP2:
SP3: - - - -
SP4: - - - -
SP5: - - - - -
SP6: - -
SP7:
SP8:
SP9: - - -
SP10: - -
SP11:
SP12: -
SP13:
SP14:

ExUns: 14 ^ 15 ^ 16a · 16b ^ 17 ^ 18 ^ 19 ^ 20 ^ 21 ^ 22a · 22b ^ 23a · 23b ^ 24 ^ 25
SP1: - -
SP2:
SP3:
SP4:
SP5:
SP6:
SP7:
SP8:
SP9:
SP10:
SP11:
SP12:
SP13:
SP14:

Relevant External Links for RNF20 Gene

GeneLoc Exon Structure for
RNF20
ECgene alternative splicing isoforms for
RNF20

Expression for RNF20 Gene

mRNA expression in normal human tissues for RNF20 Gene

Protein differential expression in normal tissues from HIPED for RNF20 Gene

This gene is overexpressed in Peripheral blood mononuclear cells (18.6) and Lymph node (13.8).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for RNF20 Gene



SOURCE GeneReport for Unigene cluster for RNF20 Gene Hs.729085

mRNA Expression by UniProt/SwissProt for RNF20 Gene

Q5VTR2-BRE1A_HUMAN
Tissue specificity: Expressed in the normal brain and also in malignant gliomas (at protein level).
genes like me logo Genes that share expression patterns with RNF20: view

Protein tissue co-expression partners for RNF20 Gene

- Elite partner

Primer Products

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery and mRNA differential expression in normal tissues for RNF20 Gene

Orthologs for RNF20 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for RNF20 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia RNF20 35
  • 92.51 (n)
  • 98.05 (a)
RNF20 36
  • 98 (a)
OneToOne
dog
(Canis familiaris)
Mammalia RNF20 35
  • 92.79 (n)
  • 98.46 (a)
RNF20 36
  • 98 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Rnf20 35
  • 90.34 (n)
  • 97.84 (a)
Rnf20 16
Rnf20 36
  • 98 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia RNF20 35
  • 99.69 (n)
  • 100 (a)
RNF20 36
  • 100 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Rnf20 35
  • 89 (n)
  • 97.23 (a)
oppossum
(Monodelphis domestica)
Mammalia RNF20 36
  • 94 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia RNF20 36
  • 94 (a)
OneToOne
chicken
(Gallus gallus)
Aves RNF20 35
  • 79.77 (n)
  • 90.04 (a)
RNF20 36
  • 90 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia -- 36
  • 97 (a)
OneToMany
-- 36
  • 85 (a)
OneToMany
tropical clawed frog
(Silurana tropicalis)
Amphibia rnf20 35
  • 69.32 (n)
  • 76.1 (a)
Str.444 35
zebrafish
(Danio rerio)
Actinopterygii Dr.6244 35
rnf20 35
  • 72.08 (n)
  • 77.31 (a)
rnf20 36
  • 59 (a)
OneToOne
fruit fly
(Drosophila melanogaster)
Insecta CG10542 37
  • 46 (a)
Bre1 36
  • 41 (a)
OneToMany
worm
(Caenorhabditis elegans)
Secernentea R05D3.4 37
  • 26 (a)
rfp-1 36
  • 24 (a)
OneToMany
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes BRE1 36
  • 21 (a)
OneToMany
sea squirt
(Ciona savignyi)
Ascidiacea CSA.10524 36
  • 40 (a)
OneToMany
Species with no ortholog for RNF20:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for RNF20 Gene

ENSEMBL:
Gene Tree for RNF20 (if available)
TreeFam:
Gene Tree for RNF20 (if available)

Paralogs for RNF20 Gene

Paralogs for RNF20 Gene

(1) SIMAP similar genes for RNF20 Gene using alignment to 6 proteins:

genes like me logo Genes that share paralogs with RNF20: view

Variants for RNF20 Gene

Sequence variations from dbSNP and Humsavar for RNF20 Gene

SNP ID Clin Chr 09 pos Sequence Context AA Info Type MAF
rs728965 -- 101,563,399(+) TGATT(C/T)TATAT downstream-variant-500B
rs1885977 -- 101,545,593(-) TAGTA(A/T)ACAAA intron-variant
rs2273976 -- 101,547,163(-) GCTCC(A/G)TACAC synonymous-codon, reference
rs4742818 -- 101,550,165(+) GTTAA(A/C)TCAGT intron-variant
rs4743471 -- 101,550,065(+) TCTTT(C/T)GATCA intron-variant

Structural Variations from Database of Genomic Variants (DGV) for RNF20 Gene

Variant ID Type Subtype PubMed ID
nsv893626 CNV Loss 21882294
nsv893627 CNV Loss 21882294
nsv831672 CNV Gain 17160897

Variation tolerance for RNF20 Gene

Residual Variation Intolerance Score: 7.65% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.40; 42.47% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for RNF20 Gene

HapMap Linkage Disequilibrium report
RNF20
Human Gene Mutation Database (HGMD)
RNF20

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for RNF20 Gene

Disorders for RNF20 Gene

Relevant External Links for RNF20

Atlas of Genetics and Cytogenetics in Oncology and Haematology:
RNF20
genes like me logo Genes that share disorders with RNF20: view

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for RNF20 Gene

Publications for RNF20 Gene

  1. The histone H2B-specific ubiquitin ligase RNF20/hBRE1 acts as a putative tumor suppressor through selective regulation of gene expression. (PMID: 18832071) Shema E. … Oren M. (Genes Dev. 2008) 2 23 67
  2. Human BRE1 is an E3 ubiquitin ligase for Ebp1 tumor suppressor. (PMID: 19037095) Liu Z. … Ye K. (Mol. Biol. Cell 2009) 2 67
  3. The human homolog of yeast BRE1 functions as a transcriptional coactivator through direct activator interactions. (PMID: 16337599) Kim J. … Roeder R.G. (Mol. Cell 2005) 2 67
  4. The Paf1 complex is essential for histone monoubiquitination by the Rad6-Bre1 complex, which signals for histone methylation by COMPASS and Dot1p. (PMID: 12876294) Wood A. … Shilatifard A. (J. Biol. Chem. 2003) 2 67
  5. The BioPlex Network: A Systematic Exploration of the Human Interactome. (PMID: 26186194) Huttlin E.L. … Gygi S.P. (Cell 2015) 67

Products for RNF20 Gene

Sources for RNF20 Gene

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